Bioefficacy of Epaltes divaricata (L.) n-Hexane Extracts and Their Major Metabolites against the Lepidopteran Pests Spodoptera litura (fab.) and Dengue Mosquito Aedes aegypti (Linn.)

The present research investigated the chemical characterization and insecticidal activity of n-Hexane extracts of Epaltes divaricata (NH-EDx) along with their chief derivatives n-Hexadecanoic acid (n-HDa) and n-Octadecanoic acid (n-ODa) against the dengue vector Aedes aegypti and lepidopteran pest Spodoptera litura. Chemical screening of NH-EDx through GC–MS analysis delivered nine major derivatives, and the maximum peak area percentage was observed in n-Hexadecanoic acid (14.63%) followed by n-Octadecadienoic acid (6.73%). The larvicidal activity of NH-EDx (1000 ppm), n-HDa (5 ppm), and n-ODa (5 ppm) against the A. aegypti and S. litura larvae showed significant mortality rate in a dose-dependent way across all the instars. The larvicidal activity was profound in the A. aegypti as compared to the S. litura across all the larval instars. The sublethal dosages of NH-EDx (500 ppm), n-HDa (2.5 ppm), and n-ODa (2.5 ppm) also showed alterations in the larval/pupal durations and adult longevity in both the insect pests. The enzyme activity revealed that the α- and β-carboxylesterase levels were decreased significantly in both the insect pests, whereas the levels of GST and CYP450 uplifted in a dose-dependent manner of NH-EDx, n-HDa, and n-ODa. Correspondingly, midgut tissues such as the epithelial layer (EL), gut lumen (GL), peritrophic matrix (Pm), and brush border membrane (BBM) were significantly altered in their morphology across both A. aegypti and S. litura against the NH-EDx and their bioactive metabolites. NH-EDx and their bioactive metabolites n-HDa and n-ODa showed significant larvicidal, growth retardant, enzyme inhibition, and midgut toxicity effects against two crucial agriculturally and medically challenging insect pest of ecological importance.

medicinal plant used in Ayurveda medicine for healing diverse medical problems [35,36]. Previous research on ethyl acetate extracts of E. divaricata displayed profound inhibition against Staphylococcus aureus multiple drug resistant strains (MRSA) [37]. However, the chemical characterization of these extracts in screening their phytochemicals and their insecticidal activity remains scanty.
Thus, the present research aimed to investigate the following objectives: (a) chemical characterization and larvicidal activity of n-Hexane extracts of E. divaricata (NH-EDx), along with their chief derivatives n-Hexadecanoic acid (n-HDa) and n-Octadecanoic acid (n-ODa), against A. aegypti and S. litura; (b) detection of developmental changes in the larval and pupal duration in both insect pests; (c) detection of enzyme inhibition activity of NH-EDx, n-HDa, and n-ODa in both lepidopteran pest and dengue vector and (d) evaluation of gut-histological activity in both of the pests after treatment with the extracts and major derivatives of E. divaricata.

Insect Culture
A. aegypti rearing was maintained unexposed to any chemicals at the Insect Toxicology Laboratory (ITL) at St. Peter's Institute of Higher Education and Research, Chennai, India, and the complete rearing procedure followed the adapted methodology of Thanigaivel et al. [19]. The cultures were conserved, and all the experiments were carried out in our laboratory at 27 ± 2 • C and 75-85% RH under a 14:10 L/D photoperiod without exposure to any chemical insecticides. Brewer's yeast, dog biscuits (Choo Stix Biskies), and algae collected from pools in a ratio of 3:2 were fed as a diet to the larvae. Pupae that emerged from the larvae were shifted to a plastic cup (round, 250 mL capacity) containing tap water and located in breeding cages (60 × 60 × 60 cm dimensions) for adult emergence. Wet raisins (dried grapes) and 10% sucrose solution soaked in cotton were fed to the adults. The adult females were destitute of sucrose from 6h and then provided a mouse placed in a breeding cage overnight for blood feeding. Adult mosquitoes were maintained under sterile conditions as similar to the larvae. The first-generation larvae were used for conducting the experiment.
Correspondingly, the S. litura culture was maintained since 2019 without exposure to any pesticides or chemicals under in vitro conditions. The eggs were sterilized in surface using 10% formaldehyde for 10 min and washed with double distilled water for 5 min, then finally air-dried and allowed to hatch. The adults that emerged were shifted to a hygienic plastic container having castor leaves for oviposition and were fed with 8% honey solution on cotton wool located in a small glass cap. Generally, eggs laid in batches on paper were kept for hatching in sterilized plastic boxes. The environment was set as 25-27 • C and 60-70% relative humidity in an ecologically controlled chamber. The day/night period was adjusted to 13:11 h. Second-generation larval instars were used for the bioassay.

Plant Harvesting and Crude Extract Preparation
Fresh leaves of the plant material Epaltes divaricata (L.) Cass. were harvested during the early morning hours (6.00 AM) from the Southern Ghats region, Tirunelveli, Tamil Nadu, India. Harvesting was authenticated by a botanist from Plant Biology and Plant Biotechnology, Presidency College, Chennai, India (Voucher Specimen No.00629), and leaves were deposited in the Department of Botany, Captain Srinivasa Murti Drug Research Institute for Ayurveda, Chennai, India for reference. The external morphology of E. divaricata is displayed in Figure 1.
The collected plant material was shade-dried under laboratory conditions (27 ± 2 °C). The dried leaves were powdered finely using a blender, and the dried leaf powder was extracted by a a Soxhlet apparatus using n-hexane solvent as adapted from Ponsankar et al. [11]; Further, 500 g air-dried plant powder was dissolved with 700 mL of n-hexane (60-80 °C) and then extracted with 750 mL distilled n-hexane in a Soxhlet apparatus for 16 h. The extract was filtered and concentrated in a rotary flash evaporator at 60 °C; the concentrated n-hexane extract was poured into the same volume of methanol while stirring and then filtered. The solvent was further removed by vacuum evaporation in a rotary evaporator, and the deposits were stored at 4 °C in an airtight glass bottle for further experiments. The yield of n-hexane extract was 2.03 g.

Characterization of Plant Volatile Through GC-MS
Two microliters of NH-EDx were dissolved in methanol (HPLC-grade) and projected to GC and MS JEOL GC mate prepared with secondary electron multiplier (JEOL GCMATE II GC-MS (Agilent Technologies 6890N Network GC system)). The HP5 column was linked with silica 55 m × 0.25 mm I.D. Analysis conditions were i) HPLC-grade-20 min at 100 °C; ii) column temperature-3 min at 235 °C; and iii) injector temperature 240 °C. Helium was the carrier gas, and the split ratio was 5:4. Further experimental procedure was adapted from the previous methodology [37]. The molecular structure, molecular weight, and formula of the individual compounds of test materials were ascertained by interpretation of the mass spectrum from GC-MS using the database of the National Institute of Standards and Technology (NIST).

Chemicals
GC-MS analysis results of NH-EDx delivered peak areas in n-Hexadecanoic acid (n-HDa) followed by n-Octadecadienoic acid (n-ODa). Thus, to detect the efficacy of these selective metabolites of NH-EDx against insect pests, the plant chemicals were commercially purchased from Sigma-Aldrich (≥99% (capillary GC)).

A. aegypti Larvicidal Assay
The second, third, and fourth instar larvae were placed into 200 mL sterile disposable plastic cups each covering 20 mL of different dosages of NH-EDx (200, 400, 600, 800, and The collected plant material was shade-dried under laboratory conditions (27 ± 2 • C). The dried leaves were powdered finely using a blender, and the dried leaf powder was extracted by a a Soxhlet apparatus using n-hexane solvent as adapted from Ponsankar et al. [11]; Further, 500 g air-dried plant powder was dissolved with 700 mL of n-hexane (60-80 • C) and then extracted with 750 mL distilled n-hexane in a Soxhlet apparatus for 16 h. The extract was filtered and concentrated in a rotary flash evaporator at 60 • C; the concentrated n-hexane extract was poured into the same volume of methanol while stirring and then filtered. The solvent was further removed by vacuum evaporation in a rotary evaporator, and the deposits were stored at 4 • C in an airtight glass bottle for further experiments. The yield of n-hexane extract was 2.03 g.

Characterization of Plant Volatile through GC-MS
Two microliters of NH-EDx were dissolved in methanol (HPLC-grade) and projected to GC and MS JEOL GC mate prepared with secondary electron multiplier (JEOL GCMATE II GC-MS (Agilent Technologies 6890N Network GC system)). The HP5 column was linked with silica 55 m × 0.25 mm I.D. Analysis conditions were (i) HPLC-grade-20 min at 100 • C; (ii) column temperature-3 min at 235 • C; and (iii) injector temperature 240 • C. Helium was the carrier gas, and the split ratio was 5:4. Further experimental procedure was adapted from the previous methodology [37]. The molecular structure, molecular weight, and formula of the individual compounds of test materials were ascertained by interpretation of the mass spectrum from GC-MS using the database of the National Institute of Standards and Technology (NIST).

Chemicals
GC-MS analysis results of NH-EDx delivered peak areas in n-Hexadecanoic acid (n-HDa) followed by n-Octadecadienoic acid (n-ODa). Thus, to detect the efficacy of these selective metabolites of NH-EDx against insect pests, the plant chemicals were commercially purchased from Sigma-Aldrich (≥99% (capillary GC)).

Larvicidal Bioassay
The second, third, and fourth instar larvae were placed into 200 mL sterile disposable plastic cups each covering 20 mL of different dosages of NH-EDx (200, 400, 600, 800, and 1000 ppm) and the bioactive derivatives n-HDa and n-ODa (1, 2, 3, 4, and 5 ppm) and incubated at 27 • C. A solution containing n-Hexane (0.1%) was employed as the negative control. During the treatment period, larval food was added to each test cup, particularly if high mortality was noted in the control. Larvae were considered dead when they were unable to reach the surface of the solution when the cups were disturbed. The larvae were considered moribund if, at the end of 24 h, they showed no sign of swimming movements even after gentle touching with a glass rod. The dead and moribund larvae were recorded after 24 h of treatment. Larvicidal assay was adapted from the World Health Organization [38].

S. litura Larvicidal Assay
Larvicidal assays of NH-EDx and their derivatives n-HDa and n-ODa were executed against the II, III, and IV instars of S. litura larvae with discriminating dosages utilized in the A. aegypti larvicidal assay (Section 2.5.1). The treatment dosages were gushed in sterile castor leaves and allowed to air dry. Hexane (0.1%) was used alone as a control. Second to fourth instars larvae were starved for 4 h were exposed to the treated leaves and kept under fixed conditions (28 ± 2 • C and 75% relative humidity). The rate of mortality was observed every 24 h, compared with the control, and recorded. The calculated mortality data percentage was examined using Probit analysis to determine the lethal concentrations (LC 50 and LC 90 ). The treatments were replicated five times, and each replicated set contained one control. Analyses of the follow-up assay were carried out according to the Probit method. Percentage mortality (1) was calculated by using Abbott's formula [39] (2).
Percentage of mortality = Number of dead larvae Number of larvae introduced × 100 (1) Corrected percentage of mortality = 1 − n in T after treatment n in C after treatment × 100 (2)

Larval and Pupal Duration Assay
The effect of sublethal dosages (LC 50 -dosage responsible to cause 50% insect mortality in a single exposure) of plant extracts (NH-EDx) along with their major phytochemicals (n-HDa and n-ODa) against the larval and pupal stages of both A. aegypti and S. litura, with discriminating sublethal dosages (NH-EDx-100, 200, 300, 400, and 500 ppm; n-HDa and n-ODa-0.5, 1.0, 1.5, and 2.0 ppm) were prepared. For analyzing A. aegypti larval and pupal durations, the sublethal dosages were subjected in an enamel tray of 30 × 25 × 5 cm dimensions. Fifty eggs were released into enamel tray in treated water and allowed to hatch. For analyzing S. litura larval and pupal duration, the sublethal dosages were dipped in sterile castor leaves, the eggs were released, and their duration to become larvae and pupae in days were calculated and compared with the control, which was treated with hexane alone. The total larval duration (days) was calculated from hatching to pupation. Pupae were placed in a small container closed with a transparent mesh. The pupal duration (days) was calculated from the pupal molt to adult emergence. The enzyme activity of αand β-carboxylesterase in the A. aegypti and S. litura was determined using the adapted methodology of Agra-Neto et al. [40], The enzyme activity was investigated by the expression of one unit of active enzyme, which was determined as the amount of enzyme essential to generate 1 µmol of αor β-naphthol expressed per minute.

GST and CYP450 Activity
Glutathione-S transferase activity (GST) was expressed as µmol/mg protein/min substrate conjugated, and cytochrome P450 activity (CYP450) was expressed as l mol 7-OH/mg larvae/min. The complete protocol was followed based on the GST assay kit (Sigma-Aldrich (Catalog 0410, Bangalore)) that was used to evaluate the conjugation of the thiol group of glutathione to the 1-chloro-2, 4-dinotrobenzene (CDNB) substrate. CYP450 activity was determined based on the measurement of ethoxycoumarin-O-de-ethylase level in the body walls as adapted from Thanigaivel et al. [41].

Gut-Histological Assay
The NH-EDx-, n-HDa-, n-ODa-, treated and control-fourth instars of A. aegypti and S. litura were fixed in Bouin's solution overnight, flowingly dehydrated, and mounted in wax blocks using paraffin. Larval blocks of tissue were segmented using the microtome instrument (Leica, Germany), and the active sections were mounted on sterile microscopic glass slides, stained with eosin and hematoxylin, and observed for histopathological changes and photographed using light a microscope (Optika vision lite 2.0 ML) preconnected with a laptop. Midgut cells of A. aegypti and S. litura were photographed, and the changes in the midgut cells of treated larvae were observed and matched with control [6].

Data Analysis
The larvicidal experimental data were exposed to analysis of variance (ANOVA and square root transformed percentages), and the obtained statistical results were considered as a five replicate means. Statistical significance within each larvicidal group was analyzed using Tukey's multiple range test (significance at p < 0.05), and the lethal concentrations of larvae in 24 h were calculated by Probit analysis with a dependability interval of 95% using the Minitab ® 17 program. For determining enzyme inhibition level, Microcal Software (Sigma plot 11) was used to plot the graphs.

Chemical Characterization of NH-EDx
Chemical screening of NH-EDx through GC-MS analysis showed nine major derivatives, and the maximum peak area percentage was observed in n-Hexadecanoic acid (14.63%) followed by n-Octadecadienoic acid (6.73%). Correspondingly, the NH-EDx was also composed of Hexacosane (6.13%), n-Hexatriacontane (5.13%), Ethyl Hexadecanoate (1.32%), Tetratriacontane (1.3%), n-Eicosane (1.13%), Neophytadiene (0.83%), and finally Hexadecane (0.75%) ( Table 1). All of these derivatives might play a significant role in causing mortality rate in both of the pests. Since these metabolites are the major active derivatives of the NH-EDx, they all synergistically act together, which results in displaying profound activity against both the pests. Despite this, the major peak area derivatives n-HDa followed by n-ODa might signify a profound level of contribution against the insect pests. Thus, these two compounds were purchased commercially, and their bioefficacy against both the pests (A. aegypti and S. litura) was examined parallel to that of the NH-EDx. strate conjugated, and cytochrome P450 activity (CYP OH/mg larvae/min. The complete protocol was follow (Sigma-Aldrich (Catalog 0410, Bangalore) that was used t thiol group of glutathione to the 1-chloro-2, 4-dinotroben activity was determined based on the measurement o level in the body walls as adapted from Thanigaivel et al

Gut-Histological Assay
The NH-EDx-, n-HDa-, n-ODa-, treated and control S. litura were fixed in Bouin's solution overnight, flowin wax blocks using paraffin. Larval blocks of tissue were instrument (Leica, Germany), and the active sections wer glass slides, stained with eosin and hematoxylin, and changes and photographed using light a microscope (Op nected with a laptop. Midgut cells of A. aegypti and S. lit changes in the midgut cells of treated larvae were observ

Data Analysis
The larvicidal experimental data were exposed to an square root transformed percentages), and the obtained s as a five replicate means. Statistical significance within ea using Tukey's multiple range test (significance at p < 0.0 of larvae in 24 h were calculated by Probit analysis with using the Minitab ® 17 program. For determining enzym ware (Sigma plot 11) was used to plot the graphs.

Chemical Characterization of NH-EDx
Chemical screening of NH-EDx through GC-MS an atives, and the maximum peak area percentage was o (14.63%) followed by n-Octadecadienoic acid (6.73%). Co also composed of Hexacosane (6.13%), n-Hexatriacontan (1.32%), Tetratriacontane (1.3%), n-Eicosane (1.13%), Ne Hexadecane (0.75%) ( Table 1). All of these derivatives mig ing mortality rate in both of the pests. Since these metabo tives of the NH-EDx, they all synergistically act together found activity against both the pests. Despite this, the ma followed by n-ODa might signify a profound level of con Thus, these two compounds were purchased commercia both the pests (A. aegypti and S. litura) was examined par activity was determined based on the measurement of level in the body walls as adapted from Thanigaivel et al.

Gut-Histological Assay
The NH-EDx-, n-HDa-, n-ODa-, treated and control-S. litura were fixed in Bouin's solution overnight, flowing wax blocks using paraffin. Larval blocks of tissue were se instrument (Leica, Germany), and the active sections were glass slides, stained with eosin and hematoxylin, and changes and photographed using light a microscope (Opt nected with a laptop. Midgut cells of A. aegypti and S. litu changes in the midgut cells of treated larvae were observe

Data Analysis
The larvicidal experimental data were exposed to ana square root transformed percentages), and the obtained sta as a five replicate means. Statistical significance within each using Tukey's multiple range test (significance at p < 0.05 of larvae in 24 h were calculated by Probit analysis with a using the Minitab ® 17 program. For determining enzyme ware (Sigma plot 11) was used to plot the graphs.

Chemical Characterization of NH-EDx
Chemical screening of NH-EDx through GC-MS ana atives, and the maximum peak area percentage was obs (14.63%) followed by n-Octadecadienoic acid (6.73%). Corr also composed of Hexacosane (6.13%), n-Hexatriacontane (1.32%), Tetratriacontane (1.3%), n-Eicosane (1.13%), Neop Hexadecane (0.75%) ( Table 1). All of these derivatives migh ing mortality rate in both of the pests. Since these metabol tives of the NH-EDx, they all synergistically act together, w found activity against both the pests. Despite this, the maj followed by n-ODa might signify a profound level of contr Thus, these two compounds were purchased commerciall both the pests (A. aegypti and S. litura) was examined para

Larvicidal Activity
The larvicidal activity of NH-EDx against A. aegypti larv tality rate in a dose-dependent way across all the instars. The

Larvicidal Activity
The larvicidal activity of NH-EDx against A. aegypti tality rate in a dose-dependent way across all the instars.

Larvicidal Activity
The larvicidal activity of NH-EDx against A. aegypti larv tality rate in a dose-dependent way across all the instars. The

Larvicidal Activity
The larvicidal activity of NH-EDx against A. aegypti l tality rate in a dose-dependent way across all the instars. T
The larvicidal activity of NH-EDx against the lepidopteran pest S. litura showed a prominent mortality rate at the maximum lethal dosage of 1000 ppm across the II instars   Overall, the larval mortality rate was found to be higher in the A. aegypti than in the S. litura across all the dosages; the maximum significance was observed in the 1000 ppm dosage (F 4,20 = 15.33, p ≤ 0.001). Also, the mortality rate of phytocompounds seems to be profound against the A. aegypti larvae as compared to the NH-EDx. In contrast, the larval mortality was significant in the crude extract (NH-EDx) as compared to the phytocompounds (n-HDa and n-ODa) against S. litura larvae. The LC 50 (lethal concentration to kill 50% of larvae) was observed at 500 ppm in the NH-EDx and 2.5 ppm in the n-HDa and n-ODa across both pests.

Developmental Changes
Parallel to larval mortality, the sublethal dosages of NH-EDx (500 ppm), n-HDa (2.5 ppm), and n-ODa (2.5 ppm) also significantly decreased the growth rates of larvae and pupae of both A. aegypti and S. litura. The larval and pupal duration of S. litura after treatment with NH-EDx (500 ppm) increased significantly as compared to other treatment concentrations and control (larvae: F 4,20 = 11.33, p ≤ 0.001; pupae: F 4,20 = 17.90, p ≤ 0.001) ( Figure 4A,D). Correspondingly, the n-HDa sublethal dosage also showed significant upsurge in the larval and pupal duration of S. litura at the maximum dosage of 2.5 ppm in both larvae (F 4,20 = 9.13, p ≤ 0.001) and pupae (F 4,20 = 10.63, p ≤ 0.001) ( Figure 4B,E). Similar results were observed in the n-ODa treatment, as the larval and pupal duration of S. litura extended prominently when the treatment dosage was increased. However, there was no significant difference in the larval and pupal growth rates between the control and the 0.5 ppm dosage (F 4,20 = 8.13, p ≤ 0.001) ( Figure 4C,F).

Developmental Changes
Parallel to larval mortality, the sublethal dosages of NH-EDx (500 ppm), n-HDa (2.5 ppm), and n-ODa (2.5 ppm) also significantly decreased the growth rates of larvae and pupae of both A. aegypti and S. litura. The larval and pupal duration of S. litura after treatment with NH-EDx (500 ppm) increased significantly as compared to other treatment concentrations and control (larvae: F4,20 = 11.33, P ≤ 0.001; pupae: F4,20 = 17.90, P ≤ 0.001) ( Figure  4A,D). Correspondingly, the n-HDa sublethal dosage also showed significant upsurge in the larval and pupal duration of S. litura at the maximum dosage of 2.5 ppm in both larvae (F4,20 = 9.13, P ≤ 0.001) and pupae (F4,20 = 10.63, P ≤ 0.001) ( Figure 4B,E). Similar results were observed in the n-ODa treatment, as the larval and pupal duration of S. litura extended prominently when the treatment dosage was increased. However, there was no significant difference in the larval and pupal growth rates between the control and the 0.5 ppm dosage (F4,20 = 8.13, P ≤ 0.001) (Figure 4 C,F).
The larval and pupal duration of A. aegypti significantly increased when the treatment dosage of NH-EDx, n-HDa and n-ODa was increased. The duration of both larvae and pupae was profound in the maximum sublethal dosage of 500 ppm of NH-EDx   The larval and pupal duration of A. aegypti significantly increased when the treatment dosage of NH-EDx, n-HDa and n-ODa was increased. The duration of both larvae and pupae was profound in the maximum sublethal dosage of 500 ppm of NH-EDx (larvae: F 4,20 = 28.94, p ≤ 0.001; pupae-F 4,20 = 14.88, p ≤ 0.001), and it was significant compared with other treatment dosages and the control ( Figure 5A,D). Similar trends were observed in the n-HDa treatment, as the maximum sublethal dosage (2.5 ppm and 2.0 ppm) increased the larval and pupal days of A. aegypti steadily as compared to other treatment dosages (Figure 5 B,E). However, there was no significant difference in the duration of larvae and pupae in A. aegypti from 0.5 to 1.5 ppm dosages of n-ODa (larvae-F 4,20 = 8.54, p ≤ 0.001; pupae-F 4,20 = 7.32, p ≤ 0.001) ( Figure 5C,F). Correspondingly, the effect on adult longevity (male and female) was also found to be profound in S. litura larvae treated with 500 ppm NH-EDx (F4,20 = 18.32, P ≤ 0.001) (Figure 6A), 2.5 ppm n-HDa (F4,20 = 13.45, P ≤ 0.001) ( Figure 6B) and 2.5 ppm n-ODa (F4,20 = 15.77, P ≤ 0.001) ( Figure 6C). Likewise, the longevity of adults in A. aegypti displayed a significant decline in a dose-dependent manner. Both male and female longevity significantly decreased in 500 ppm NH-EDx (F4,20 = 14.88, P ≤ 0.001) ( Figure 6D Correspondingly, the effect on adult longevity (male and female) was also found to be profound in S. litura larvae treated with 500 ppm NH-EDx (F 4,20 = 18.32, p ≤ 0.001) ( Figure 6A), 2.5 ppm n-HDa (F 4,20 = 13.45, p ≤ 0.001) ( Figure 6B) and 2.5 ppm n-ODa (F 4,20 = 15.77, p ≤ 0.001) ( Figure 6C). Likewise, the longevity of adults in A. aegypti displayed a significant decline in a dose-dependent manner. Both male and female longevity significantly decreased in 500 ppm NH-EDx (F 4,20 = 14.88, p ≤ 0.001) ( Figure 6D

α-Carboxylesterase
The sublethal dosage of NH-EDx significantly controlled the level of α-carboxylesterase activity in S. litura fourth instar larvae in a concentration-dependent manner. The level of α-carboxylesterase reduced at the maximum dosage of 500 ppm (F4,20 = 13.55, P ≤ 0.001), which result was significant against other treatment dosages and control ( Figure  7A). Likewise, the maximum dosages of n-HDa and n-ODa (3.0 ppm) also exhibited strong inhibition in the level of α-carboxylesterase as compared to other treatment dosages and control (F4,20 = 19.77, P ≤ 0.001; and F4,20 = 23.11, P ≤ 0.001, respectively) (Figure 7 B&C). The maximum sublethal dosage of NH-EDx (500 ppm) also reduced the level of α-carboxylesterase in A. aegypti fourth instar larvae significantly as compared to other treatment concentrations. However, there was no significant difference between control and 300 ppm NH-EDx (F4,20 = 18.22, P ≤ 0.001) ( Figure 7D). The prominent sublethal dosage of n-HDa

α-Carboxylesterase
The sublethal dosage of NH-EDx significantly controlled the level of α-carboxylesterase activity in S. litura fourth instar larvae in a concentration-dependent manner. The level of α-carboxylesterase reduced at the maximum dosage of 500 ppm (F 4,20 = 13.55, p ≤ 0.001), which result was significant against other treatment dosages and control ( Figure 7A). Likewise, the maximum dosages of n-HDa and n-ODa (3.0 ppm) also exhibited strong inhibition in the level of α-carboxylesterase as compared to other treatment dosages and control (F 4,20 = 19.77, p ≤ 0.001; and F 4,20 = 23.11, p ≤ 0.001, respectively) (Figure 7 B&C). The maximum sublethal dosage of NH-EDx (500 ppm) also reduced the level of α-carboxylesterase in A. aegypti fourth instar larvae significantly as compared to other treatment concentrations. However, there was no significant difference between control and 300 ppm NH-EDx (F 4,20 = 18.22, p ≤ 0.001) ( Figure 7D). The prominent sublethal dosage of n-HDa (3.0 ppm) significantly decreased the enzyme rate as compared to other treatment concentrations and control (F 4,20 = 19.44, p ≤ 0.001) ( Figure 7E). Similar trends were observed in n-ODa treatment (3.0 ppm), which reduced the α-carboxylesterase in the fourth instars of A. aegypti larvae. However, there was no significant difference between the control and the 1.0 ppm dosage (F 4,20 = 23.22, p ≤ 0.001) ( Figure 7F). A. aegypti larvae. However, there was no significant difference between the control and the 1.0 ppm dosage (F4,20 = 23.22, P ≤ 0.001) ( Figure 7F).

β-Carboxylesterase
The level of β-carboxylesterase in the fourth instars of S. litura was significantly reduced by treatment with sublethal dosages of NH-EDx, n-HDa, and n-ODa, with maximum reductions at 500 ppm (F 4,20 = 17.47, p ≤ 0.001) ( Figure 8A

Gut-Histological Activity
The midgut cells were severely infected in the fourth instar larvae of A. aegypti after treatment with sublethal dosages of NH-EDx (500 ppm) ( Figure 11B), n-HDa ( Figure 11C), and n-ODa ( Figure 11D) as compared to the control ( Figure 11A). The control midgut cells appeared normal, with a clearly visible epithelial layer (EL), well distinguished gut lumen (GL), and normal peritrophic matrix (Pm). Midgut cells treated with NH-EDx and their major phytochemicals showed severe morphological alterations in the GL, EL, and Pm. The damage rate was particularly severe for NH-EDx as compared to its metabolites n-HDa and n-ODa. Correspondingly, the fourth instar larval midgut cells of S. litura were significantly affected after treatment with sublethal dosages of NH-EDx ( Figure 11F), n-HDa ( Figure 11G), and n-ODa ( Figure 11H) as compared to the control ( Figure 11E). Moreover, the brush border membrane (BBM), EL, and GL displayed the most significant cellular damage in NH-EDx, followed by n-HDa and n-ODa.

Gut-Histological Activity
The midgut cells were severely infected in the fourth instar larvae of A. aegypti after treatment with sublethal dosages of NH-EDx (500 ppm) ( Figure 11B), n-HDa ( Figure 11C), and n-ODa ( Figure 11D) as compared to the control ( Figure 11A). The control midgut cells appeared normal, with a clearly visible epithelial layer (EL), well distinguished gut lumen (GL), and normal peritrophic matrix (Pm). Midgut cells treated with NH-EDx and their major phytochemicals showed severe morphological alterations in the GL, EL, and Pm. The damage rate was particularly severe for NH-EDx as compared to its metabolites n-HDa and n-ODa. Correspondingly, the fourth instar larval midgut cells of S. litura were significantly affected after treatment with sublethal dosages of NH-EDx ( Figure 11F), n-HDa ( Figure 11G), and n-ODa ( Figure 11H) as compared to the control ( Figure 11E). Moreover, the brush border membrane (BBM), EL, and GL displayed the most significant cellular damage in NH-EDx, followed by n-HDa and n-ODa.

Discussion
It is marked that chemical-based insecticides/pesticides are having less impact against both mosquitoes and agriculture pests due to the upsurge in resistance of insect pests of different classes against commercial chemicals [42,43]. Synthetic insecticide resistance has been linked to the increased metabolism level in the insects, including the uplift of detoxifying enzymes or enzyme structural changes that increase the capacity of the metabolic rate [43]. The major factors that are responsible for insecticidal resistance include biological factors: (i) life cycle of the insect, (ii) population growth rate, (iii) genetics of the insect, and behavioral factors: (iv) movement and more importantly (v) exposure

Discussion
It is marked that chemical-based insecticides/pesticides are having less impact against both mosquitoes and agriculture pests due to the upsurge in resistance of insect pests of different classes against commercial chemicals [42,43]. Synthetic insecticide resistance has been linked to the increased metabolism level in the insects, including the uplift of detoxifying enzymes or enzyme structural changes that increase the capacity of the metabolic rate [43]. The major factors that are responsible for insecticidal resistance include biological factors: (i) life cycle of the insect, (ii) population growth rate, (iii) genetics of the insect, and behavioral factors: (iv) movement and more importantly (v) exposure rate to chemicals [44]. In general, commercial chemicals contain artificial blends of two or three compounds that are quickly becoming resisted by pests due to their known mode of action [44]. To overcome this problem, natural-based insecticides chiefly derived from plant sources are widely evaluated for their insecticidal efficacy against different species of both mosquito vectors and agriculture pests [45][46][47][48][49].
Plant extracts and their major derivatives play a pivotal role in managing insect pests of both agricultural and medical importance due to their significant blends of natural chemicals including phenolic compounds, alkaloids, tannins, flavonoids, steroids, terpenes, coumarins, terpenoids, and lignins [50]. Also, the active ingredients contained in them are natural substances, not synthetic substances, with smooth degradation pathways in nature after application that result in less pollution to the environment. As botanical insecticides have many insecticidal ingredients with special modes of action, it is difficult for pests to develop pesticide resistance. Also, botanical insecticides generally have features of strong selectivity; low toxicity to humans, livestock, and natural enemies; and relatively low development and use costs [50]. Deadly diseases such as dengue spread by mosquito, affect >700 million people across the nation. The major vector responsible for spreading this disease is A. aegypti, which is widespread across all tropical and subtropical nations [23]. Correspondingly, S. litura is a crucial pest of agriculture, infesting more than 70% of plant crops globally and feeds on >290 plant species [4,51,52]. Both these pests are widespread across developing countries and threaten crops and human lives.
E. divaricata belongs to the family Compositae and is widely used in traditional medicine across South Asian countries. Moreover, E. divaricata contains a blend of natural compounds, including tannins, flavonoids, and steroids, with unique modes of action against bacterial and viral infections [36]. However, their insecticidal property remains uncharted. GC-MS analysis of NH-EDx showed nine major phytochemicals, with the highest peak area observed in n-HDa followed by n-ODa. Larvicidal screening of NH-EDx showed a prominent mortality rate in both the pests S. litura and A. aegypti at the maximum dosage of 1000 ppm. Moreover, the peak area compounds of NH-EDx also showed profound mortality rate against both insect pests. Previously, hexadecanoic acid ester derivatives showed a 70-98% decline in the oviposition rate in the groundnut pest Caryedon serratus at the maximum dosage of 1.0 mg L −1 [53]. Moreover, previous research proclaims that monoterpenes, alkanes, sesquiterpenes, fatty acids, and phenyl propanoids could demonstrate significant insecticidal and repellent activity against serious pests [54]. The bioactive metabolites of NH-EDx also showed high larvicidal activity against both pests. Previously, Citrullus colocynthis L. and its chief metabolite cucurbitacin E showed significant larvicidal activity (93.8%) against S. litura [4]. In parallel, bioactive andrographolide derived from Andrographis paniculata L. showed a prominent mortality rate against the dengue mosquito vector A. aegypti [55]. Comparably to the above results, the maximum lethal dosages of NH-EDx (1000 ppm), n-HDa (5 ppm), and n-ODa (5 ppm) delivered significant mortality rates against A. aegypti and S. litura.
Sublethal concentrations of NH-EDx (500 ppm), n-HDa (2.5 ppm), and n-ODa (2.5 ppm) showed substantial impacts in the growth and development of both A. aegypti and S. litura. The larval and pupal duration were significantly higher in the crude NH-EDx as compared to their bioactive metabolites across both the insect pests. Previously, Shaalan et al. [56] illustrated that the larval duration is blocked or altered when the larva is exposed to higher range of toxic chemicals. The above statement was well matched with our present results, as a significant extension of duration in larval and pupal stages was observed as compared to the control in both insect pests. In contrast, the adult longevity of both males and females of both the insect pests showed a significant reduction in a dose dependent manner, with the highest reductions in the maximum dosages of NH-EDx (500 ppm), n-HDa (2.5 ppm), and n-ODa (2.5 ppm). Adult longevity is considered as a restricted aspect for viral transmission in arthropods specifically, as the insect releases toxins that generally need a period of time to reach the midgut lining to begin replicate and spreads through the salivary glands to transmit virus [57].
The sublethal dosages of NH-EDx and their major metabolites showed significant alterations in the levels of midgut enzymes including αand β-carboxylesterase and of detoxifying enzymes GST and CYP450 in both A. aegypti and S. litura. Previously, Koodalingam et al. [58] reported that the bioactive compounds derived from plant extracts contact to different insect species has displayed drift in the levels of carboxylesterase enzyme. The above statement was well supported with our present research, as the peak area compounds n-HDa and n-ODa displayed steady blockage in the esterase activity of both the pests. As dosage increased, αand β-carboxylesterase levels decreased, and in contrast the detoxifying enzymes GST and CYP450 increased significantly. It is well known that GST and CYP450 are recognized as crucial biomarkers to detect resistance status in any insect pest [37,59]. The present results clearly display that NH-EDx decreased the enzyme ratio significantly as compared to the individual bioactive compounds n-HDa and n-ODa, and the level was slightly higher in A. aegypti as compared to S. litura.
Examination of midgut toxicity of NH-EDx and their chief derivatives displayed that the sublethal dosages caused severe damage to gut tissues such as the peritrophic matrix and also exhibited major changes in the alignment of the epithelial layer and brush border membrane as compared to the control. Previously, Ray et al. [60] clearly stated that the primary target of any plant metabolite is the midgut epithelium cells. It is well evident that the bioactive extracts of NH-EDx showed higher toxicity in the midgut profile of both the insect pests. Amala et al. [13] proclaimed that the peritrophic membrane is chiefly responsible for growth stimulus in the pests. The major metabolites derived from the hexane extracts of Epaltes pygmaea blocked the peritrophic membrane in the dengue vector and in turn decreased the development and growth rate of the insect pests.

Conclusions
The present investigation clearly displayed that NH-EDx and their bioactive metabolites n-HDa and n-ODa showed significant larvicidal, growth retardant, enzyme inhibition, and midgut toxicity effects against the crucial agricultural and medically challenging insect pests of ecological importance. Future perspectives will be focused on detecting the resistance and susceptibility of these bioactive compounds in the targeted pests as well as detecting the nontarget impacts of n-HDa and n-ODa against beneficial organisms sharing the same ecological niche of the targeted pest. Hopefully, the effective formulation of phytochemicals as commercial botanical insecticides will replace the current chemical pesticides, which are a global burden to our society, and which create chemical resistance in different insect pests.