Oriental Hornet (Vespa orientalis) Larval Extracts Induce Antiproliferative, Antioxidant, Anti-Inflammatory, and Anti-Migratory Effects on MCF7 Cells

The use of insects as a feasible and useful natural product resource is a novel and promising option in alternative medicine. Several components from insects and their larvae have been found to inhibit molecular pathways in different stages of cancer. This study aimed to analyze the effect of aqueous and alcoholic extracts of Vespa orientalis larvae on breast cancer MCF7 cells and investigate the underlying mechanisms. Our results showed that individual treatment with 5% aqueous or alcoholic larval extract inhibited MCF7 proliferation but had no cytotoxic effect on normal Vero cells. The anticancer effect was mediated through (1) induction of apoptosis, as indicated by increased expression of apoptotic genes (Bax, caspase3, and p53) and decreased expression of the anti-apoptotic gene Bcl2; (2) suppression of intracellular reactive oxygen species; (3) elevation of antioxidant enzymes (CAT, SOD, and GPx) and upregulation of the antioxidant regulator Nrf2 and its downstream target HO-1; (4) inhibition of migration as revealed by in vitro wound healing assay and downregulation of the migration-related gene MMP9 and upregulation of the anti-migratory gene TIMP1; and (5) downregulation of inflammation-related genes (NFκB and IL8). The aqueous extract exhibited the best anticancer effect with higher antioxidant activities but lower anti-inflammatory properties than the alcoholic extract. HPLC analysis revealed the presence of several flavonoids and phenolic compounds with highest concentrations for resveratrol and naringenin in aqueous extract and rosmarinic acid in alcoholic extract. This is the first report to explain the intracellular pathway by which flavonoids and phenolic compounds-rich extracts of Vespa orientalis larvae could induce MCF7 cell viability loss through the initiation of apoptosis, activation of antioxidants, and inhibition of migration and inflammation. Therefore, these extracts could be used as adjuvants for anticancer drugs and as antioxidant and anti-inflammatory agents.


Introduction
Despite remarkable advances in chemotherapy, the numerous side effects and nonselective targeting of most of the currently used anticancer drugs restrict their therapeutic potential [1,2]. Thus, discovering novel anticancer agents with superior inhibitory effect and selective targeting to cancer cells is becoming an urgent need [3][4][5]. In recent years, targeted therapy has been searching for natural and safe medicines to avoid the side effects of anticancer drugs [6,7]. Extracts prepared from natural products and medicinal plants have notable anti-inflammatory, antioxidant and anticancer potential [3][4][5][6][7]. Similarly, many bioactive ingredients extracted from insects have demonstrated anticancer effects [8][9][10][11]. Insects and their larvae could be considered as a good source for many bioactive compounds; however, their role has not been fully investigated [12]. Previous studies have reported that some insect larvae, such as housefly larvae, possess anticancer, antioxidant, and antimicrobial properties [11,[13][14][15][16]. Additionally, aqueous extracts and hydrolysates of some insects such as house cricket, grasshopper, and silk moth contain some beneficial bioactive peptides that have anti-inflammatory and antioxidant activities [17,18]. In addition to these beneficial medicinal effects, insects are part of the common diet of at least two billion people in the world, as they contain high amounts of proteins and micronutrients [19]. Consumption of antioxidant-rich nutrients helps in preventing oxidative stress-induced diseases and cancer [5,[20][21][22][23].
Oriental hornets (Vespa orientalis) are insects that belong to the family Vespidae, which are distributed in the Middle East, Southwest Asia, Southern Europe, and Northeast Africa, and live in colonies [24]. The fertilized queens hibernate in the winter [25,26], lay their eggs during the fall [27], and the population of the colony peaks in the late summer and initial fall [28]. These insects attack bee colonies to get nectar and protein, causing notable loss to honey bee production [29,30]. Although their stings are highly painful to humans and can cause allergy, they are used for the treatment of cold and gastritis in some Indian tribes [26]. These tribes also consume various Vespa sp. due to their high protein content [31]. Moreover, the aqueous extract of lesser banded hornet (V. affinis) has been shown to exert antioxidant effects through activation of the antioxidant enzymes GST and CAT [32]. Venom extracted from V. orientalis showed a potent antimicrobial effect against a large variety of bacteria [33][34][35] and an anticancer effect [36][37][38]. One of the major drawbacks of using insect venoms as an anticancer agent is their cytotoxic and neurotoxic effects on normal cells [39,40]. Using insect larva and pupa could be a useful alternative. However, to date, there have been no reports investigating the antioxidant and anticancer effects of V. orientalis larval extracts.
Therefore, this study aimed to assess the anticancer effect of aqueous and alcoholic extracts of V. orientalis larvae against MCF7 cells and to study the underlying mechanisms through investigating apoptotic pathway, antioxidant status, migration, and inflammation.

Preparation of V. orientalis Larval Extracts
Nests of V. orientalis were collected in the fall of 2019 from different localities in the cities of Tanta and Kafrelsheikh, Egypt. The insects and nests were kept under controlled conditions with respect to temperature, relative humidity, and photoperiods in the Honey Bee Laboratory at Plant Protection Department, Faculty of Agriculture, Kafrelsheikh University. A standard rearing method was used to obtain the larvae required for the bioassay. The 3rd and 4th larvae instars were extracted using 5% water or ethyl alcohol as previously described [32,41]. In brief, the collected larvae were first washed in distilled water, and a mixture of 5 g larvae and 95 mL distilled water or pure ethyl alcohol was centrifuged (8000 rpm/20 min/4 • C) to eliminate undesirable debris. The supernatant was obtained and considered as 5% aqueous and alcoholic extracts and kept in the freezer until further use.

HPLC Analysis of Flavonoids and Phenolic Compounds
HPLC was used to analyze flavonoids and phenolic compounds in both aqueous and alcoholic extracts, as previously described [42]. The HPLC (Agilent1260 infinity HPLC Series, Agilent Technologies, Santa Clara, CA, USA) was equipped with a Quaternary pump and Kinetex 5 µm EVO C18 (100 mm × 4.6 mm) column (Phenomenex, Torrance, CA, USA) operated at 30 • C. The separation was achieved using a ternary linear elution gradient with HPLC grade water 0.2% H3P04 (v/v), methanol, and acetonitrile. The injected volume was 20 µL and the VWD detector was set at 284 nm.

Experimental Design
The cells were allocated into three groups. The untreated MCF7 cells were considered control cells (Cnt), while in the other two groups, cells were treated with aqueous or alcoholic larval extracts with doses equal to their IC 50 . Treatments were applied in triplicate at 70-80% confluence. Cells were incubated in a CO 2 incubator for 24 h at 37 • C and 95% humidity.

Estimation of Intracellular Reactive Oxygen Species
Intracellular reactive oxygen species (ROS) were determined using a fluorescent probe, 2 ,7 -dichlorofluorescein diacetate (DCFDA), as previously described [45]. MCF7 cells were treated with aqueous and alcoholic larval extracts with doses equal to their IC 50 for 2 h followed by 25 µM H 2 O 2 for 2 h. After PBS washing, DCFDA (5 µM) was added, and the cells were incubated (37 • C/30 min) in the dark. Following washing in PBS, the fluorescence intensity of DCFDA was measured in a fluorescence microplate reader. The intracellular ROS levels were calculated as a % of the control.

Detection of Antioxidant Enzymes Activities
The activities of superoxide dismutase activity (SOD), catalase (CAT), glutathione peroxidase (GPx) in MCF7 cells were assayed by colorimetric methods using commercially available kits and following the manufacturer's protocol (Biodiagnostics, Cairo, Egypt). The antioxidant activities were presented as percentages of the control.

In Vitro Scratch (Wound Healing) Assay
MCF7 cells were grown at a concentration of 2.5 × 10 5 cells/mL in 6-well plates in DMEM until 75% confluence. A scratch was produced in the center of each well and fresh media containing each extract at a concentration equal to IC 50 were added. Images were taken at two time intervals (0 h and 24 h), and MCF7 migration was calculated as previously reported [46].

Gene Expression Analysis by qPCR
RNA samples were extracted from all cells using a procedure detailed previously [6]. Nanodrop and 1% gel electrophoresis were used to determine RNA concentration and integrity, respectively. Following reverse transcription using RevertAid H Minus Reverse Transcriptase, the expression of Bax, Bcl2, caspase 3, p53, NrF2, HO-1, MMP9, TIMP1, NFκB, and IL8 genes was determined using 2X SYBR Green Master Mix and specific primers ( Table 1). All kits used in qPCR were purchased from Thermo Scientific, Waltham, MA, USA. The qPCR mixture (25 µL) contained 2 µL cDNA, 1 µL from each primer and 12.5 µL of Maxima SYBR Green Master Mix. The thermal cycling included 10 min at 95 • C followed by 45 cycles of (95 • C for 15 s, 60 • C for 30 s and 72 for 30 s). The relative expression of target genes was normalized with the housekeeping gene GAPDH and calculated using the 2 −∆∆Ct method [47].

Statistical Analysis
All data were expressed as mean ± standard error of the mean (SEM) of replicates from independent experiments. The difference between the groups was evaluated by oneway analysis of variance and Tukey's Honest Significant Difference test using GraphPad prism, 7.0 software. Values were considered statistically significant when p < 0.05.

Larval Extract Had Numerous Flavonoids and Phenolic Compounds
HPLC chromatograms for the 5% aqueous larval extract revealed the presence of numerous flavonoids (catechin, chlorogenic, rutin, quercetin, naringenin, myricetin) and phenolic compounds (vanillic acid, caffeic acid, syringic acid, ferulic acid, o-coumaric acid, resveratrol, and rosmarinic acid), as well as benzoic acid and p-hydroxybenzoic acid (a phenolic derivative of benzoic acid), p-coumaric acid, benzoic acid, and cinnamic acid. Resveratrol was the major phenolic compound (1.580 mg/kg at 19.74 min), while naringenin was the major flavonoid (0.819 mg/kg at 22.56 min) (Table 2, Figure 1A). (a phenolic derivative of benzoic acid), p-coumaric acid, benzoic acid, and cinnamic acid. Resveratrol was the major phenolic compound (1.580 mg/kg at 19.74 min), while naringenin was the major flavonoid (0.819 mg/kg at 22.56 min) (Table 2, Figure 1A).  On the other hand, the 5% alcoholic larval extract contained only catechin flavonoid and fewer phenolic compounds (vanillic acid, caffeic acid, ferulic acid, ellagic acid, and rosmarinic acid). However, the total phenolic compounds and flavonoids (45.460 mg/kg) were higher than those of the 5% aqueous larval extract (4.452 mg/kg). The major phenolic compound obtained from the alcoholic larval extract was rosmarinic acid (34.031 mg/kg at 21.83 min), which was responsible for this elevation (Table 2, Figure 1B). Looking at the HPLC chromatograms displayed in Figure 1, other unknown higher peaks can be observed that were not identified due to lack of available standards.

Larval Extract Had Potent Antioxidant Activities
The HPLC results indicated the presence of numerous flavonoids and phenolic compounds, which all had antioxidant properties. This prompted us to confirm the in vitro antioxidant activities of the two larval extracts using DPPH assay. As expected, 5% aqueous and alcoholic larval extracts had potent free radical scavenging activity, with IC50 values of 52.67 ± 2.38 and 123.50 ± 4.62 μg/mL, respectively, relative to the standard ascorbic acid (45.33 μg/mL) ( Figure 2). This suggests that the 5% aqueous larval extract had better free radical scavenging activity than the 5% alcoholic larval extract.

Aqueous Extract
Alcoholic Extract On the other hand, the 5% alcoholic larval extract contained only catechin flavonoid and fewer phenolic compounds (vanillic acid, caffeic acid, ferulic acid, ellagic acid, and rosmarinic acid). However, the total phenolic compounds and flavonoids (45.460 mg/kg) were higher than those of the 5% aqueous larval extract (4.452 mg/kg). The major phenolic compound obtained from the alcoholic larval extract was rosmarinic acid (34.031 mg/kg at 21.83 min), which was responsible for this elevation (Table 2, Figure 1B). Looking at the HPLC chromatograms displayed in Figure 1, other unknown higher peaks can be observed that were not identified due to lack of available standards.

Larval Extract Had Potent Antioxidant Activities
The HPLC results indicated the presence of numerous flavonoids and phenolic compounds, which all had antioxidant properties. This prompted us to confirm the in vitro antioxidant activities of the two larval extracts using DPPH assay. As expected, 5% aqueous and alcoholic larval extracts had potent free radical scavenging activity, with IC 50 values of 52.67 ± 2.38 and 123.50 ± 4.62 µg/mL, respectively, relative to the standard ascorbic acid (45.33 µg/mL) ( Figure 2). This suggests that the 5% aqueous larval extract had better free radical scavenging activity than the 5% alcoholic larval extract. On the other hand, the 5% alcoholic larval extract contained only catechin flavonoid and fewer phenolic compounds (vanillic acid, caffeic acid, ferulic acid, ellagic acid, and rosmarinic acid). However, the total phenolic compounds and flavonoids (45.460 mg/kg) were higher than those of the 5% aqueous larval extract (4.452 mg/kg). The major phenolic compound obtained from the alcoholic larval extract was rosmarinic acid (34.031 mg/kg at 21.83 min), which was responsible for this elevation (Table 2, Figure 1B). Looking at the HPLC chromatograms displayed in Figure 1, other unknown higher peaks can be observed that were not identified due to lack of available standards.

Larval Extract Had Potent Antioxidant Activities
The HPLC results indicated the presence of numerous flavonoids and phenolic compounds, which all had antioxidant properties. This prompted us to confirm the in vitro antioxidant activities of the two larval extracts using DPPH assay. As expected, 5% aqueous and alcoholic larval extracts had potent free radical scavenging activity, with IC50 values of 52.67 ± 2.38 and 123.50 ± 4.62 μg/mL, respectively, relative to the standard ascorbic acid (45.33 μg/mL) ( Figure 2). This suggests that the 5% aqueous larval extract had better free radical scavenging activity than the 5% alcoholic larval extract.

Aqueous Extract
Alcoholic Extract

Larval Extracts Inhibited MCF7 Viability
The results of MTT assay showed dose-dependent cytotoxic effects for 5% aqueous and alcoholic larval extracts on MCF7, with IC 50 values of 32.89 ± 1.84 and 61.48 ± 2.70 µg/mL, respectively, as compared to control (untreated) cells ( Figure 3). This suggests that the aqueous larval extract had a more potent anticancer effect than the alcoholic larval extract. However, the aqueous extract showed no cytotoxic effect on normal Vero cells up to a concentration of 100 µg/mL, and only 1% cell viability inhibition was noticed at a concentration of 200 µg/mL. The alcoholic extract also showed minimal inhibition of 0.50%, 1.5% and 4.5% at concentrations of 50, 100, and 200 µg/mL, respectively ( Figure 3).

Larval Extracts Inhibited MCF7 Viability
The results of MTT assay showed dose-dependent cytotoxic effects for 5% aqueous and alcoholic larval extracts on MCF7, with IC50 values of 32.89 ± 1.84 and 61.48 ± 2.70 μg/mL, respectively, as compared to control (untreated) cells (Figure 3). This suggests that the aqueous larval extract had a more potent anticancer effect than the alcoholic larval extract. However, the aqueous extract showed no cytotoxic effect on normal Vero cells up to a concentration of 100 μg/mL, and only 1% cell viability inhibition was noticed at a concentration of 200 μg/mL. The alcoholic extract also showed minimal inhibition of 0.50%, 1.5% and 4.5% at concentrations of 50, 100, and 200 μg/mL, respectively (Figure 3).

Larval Extracts Modulated the Expression of Apoptosis-Related Genes
The qPCR was applied to monitor the effect of treatments on the expression of the apoptotic genes (Bax, caspase3, and p53) and the anti-apoptotic gene (Bcl2). Treatment with each extract significantly upregulated the expression of the apoptotic genes and significantly downregulated the expression of Bcl2 compared to the control cells ( Figure  4). The 5% aqueous extract showed higher expression of Bax and caspase3 and lower expression of Bcl2 than the 5% alcoholic extract. These results suggest that the cytotoxic effect of the two extracts on MCF7 cells could be triggered by apoptosis. On the other hand, no significant difference was observed in any of these apoptotic or anti-apoptotic genes among the three groups in Vero cells ( Figure S1). These results imply that the two larval extracts would not induce apoptosis in normal Vero cells.

Larval Extracts Modulated the Expression of Apoptosis-Related Genes
The qPCR was applied to monitor the effect of treatments on the expression of the apoptotic genes (Bax, caspase3, and p53) and the anti-apoptotic gene (Bcl2). Treatment with each extract significantly upregulated the expression of the apoptotic genes and significantly downregulated the expression of Bcl2 compared to the control cells (Figure 4). The 5% aqueous extract showed higher expression of Bax and caspase3 and lower expression of Bcl2 than the 5% alcoholic extract. These results suggest that the cytotoxic effect of the two extracts on MCF7 cells could be triggered by apoptosis. On the other hand, no significant difference was observed in any of these apoptotic or anti-apoptotic genes among the three groups in Vero cells ( Figure S1). These results imply that the two larval extracts would not induce apoptosis in normal Vero cells.

Larval Extracts Inhibited Intracellular ROS and Induced Antioxidant Status
Given that V. orientalis larval extracts contained numerous flavonoids and phenolic compounds and had potent total antioxidant activities, we postulated that these extracts could trigger cancer cell death through decreasing oxidative stress and increasing antioxidant enzyme activities. To assess this hypothesis, we evaluated the effect of these extracts on the intracellular ROS and activities of antioxidant enzymes. Increased intracellular ROS is a notable indicator of cellular oxidative stress. As was expected, cells treated with each larval extract showed significantly lower intracellular ROS and significantly higher levels of CAT, SOD, and GPx than control cells ( Figure 5). Again, the 5% aqueous extract exhibited lower intracellular ROS and higher levels of CAT, SOD, and GPx than the 5% alcoholic extract.

Larval Extracts Inhibited Intracellular ROS and Induced Antioxidant Status
Given that V. orientalis larval extracts contained numerous flavonoids and phenolic compounds and had potent total antioxidant activities, we postulated that these extracts could trigger cancer cell death through decreasing oxidative stress and increasing antioxidant enzyme activities. To assess this hypothesis, we evaluated the effect of these extracts on the intracellular ROS and activities of antioxidant enzymes. Increased intracellular ROS is a notable indicator of cellular oxidative stress. As was expected, cells treated with each larval extract showed significantly lower intracellular ROS and significantly higher levels of CAT, SOD, and GPx than control cells ( Figure 5). Again, the 5% aqueous extract exhibited lower intracellular ROS and higher levels of CAT, SOD, and GPx than the 5% alcoholic extract. To confirm the antioxidant potential of the two extracts on a molecular basis, we studied their effect on the expression of the antioxidant-regulator NrF2 and its downstream target HO-1 using qPCR and found significant upregulation of the two genes in MCF7 cells treated with each extract, with higher expression in 5% aqueous extracttreated cells than in control cells ( Figure 6). To confirm the antioxidant potential of the two extracts on a molecular basis, we studied their effect on the expression of the antioxidant-regulator NrF2 and its downstream target HO-1 using qPCR and found significant upregulation of the two genes in MCF7 cells treated with each extract, with higher expression in 5% aqueous extract-treated cells than in control cells ( Figure 6).

Larval Extracts Inhibited MCF7 Migration
The effect of larval extracts on MCF7 migration was evaluated using a scratch (wound-healing) assay. Treatment with each larval extract significantly inhibited MCF7 migration compared to the control (Figure 7). The rates of MCF7 migration after treatment with aqueous and alcoholic extracts were 34.68 ± 2.63% and 42.28 ± 3.14%, respectively, compared to the control cells (60.38 ± 4.12%). To verify this anti-migratory effect on a molecular level, qPCR was used to check changes in the relative expression of the migration-related MMP9 and the anti-migratory TIMP1 following treatment with each larval extract. The obtained results revealed significant downregulation of MMP9 and upregulation of TIMP1 in cells treated with each extract relative to control cells ( Figure  7). No significant change in MMP9 or TIMP1 was noticed between the two extracts. Figure 6. Expression of NrF2 and HO-1 genes in MCF7 cells following treatment with 5% aqueous and alcoholic larval extracts as detected by qPCR. Data were normalized to the housekeeping gene (GAPDH) and are expressed as the mean fold change ± SEM. Samples were run in triplicate in three independent experiments, n = 5. Groups with different letters are significantly different at p < 0.05. * indicates high significance with respect to control (p < 0.001).

Larval Extracts Inhibited MCF7 Migration
The effect of larval extracts on MCF7 migration was evaluated using a scratch (woundhealing) assay. Treatment with each larval extract significantly inhibited MCF7 migration compared to the control (Figure 7). The rates of MCF7 migration after treatment with aqueous and alcoholic extracts were 34.68 ± 2.63% and 42.28 ± 3.14%, respectively, compared to the control cells (60.38 ± 4.12%). To verify this anti-migratory effect on a molecular level, qPCR was used to check changes in the relative expression of the migration-related MMP9 and the anti-migratory TIMP1 following treatment with each larval extract. The obtained results revealed significant downregulation of MMP9 and upregulation of TIMP1 in cells treated with each extract relative to control cells (Figure 7). No significant change in MMP9 or TIMP1 was noticed between the two extracts.

Larval Extracts Reduced the Expression of Inflammation-Related Genes
The inflammatory genes NFκB and IL8 exhibited a significant downregulation after the addition of each extract compared to the control cells (Figure 8). The most downregulated effect was encountered in cells treated with the 5% alcoholic extract. These findings indicate that these extracts have an anti-inflammatory effect against MCF7 cells, with the best effect being for the 5% alcoholic extract.

Larval Extracts Reduced the Expression of Inflammation-Related Genes
The inflammatory genes NFκB and IL8 exhibited a significant downregulation after the addition of each extract compared to the control cells (Figure 8). The most downregulated effect was encountered in cells treated with the 5% alcoholic extract. These findings indicate that these extracts have an anti-inflammatory effect against MCF7 cells, with the best effect being for the 5% alcoholic extract.

Discussion
There is growing interest in exploring the roles of the insects' bioactive components, especially those with antioxidant and anticancer properties. Previous studies have only investigated the anticancer effect of Vespa sp. venom and its bioactive peptides [36,37,38]. However, the effect of the venom is not selective and can damage normal cells [39,40]. Aside from the venom, insect developmental stages (larvae and pupae) exhibit antioxidant and anticancer potential, with less toxicity for normal cells. Dutta et al. [32] reported an antioxidant effect for V. affinis aqueous pupal extract. Moreover, the larval hemolymph of M. domestica had antioxidant and cytotoxic properties against MCF7, but no cytotoxicity on normal Vero cells [11]. To the best of our knowledge, this is the first study to report that 5% V. orientalis larval extracts had an anticancer effect on MCF7 cells, Figure 8. Expression of NFκB and IL8 genes in MCF7 cells following treatment with 5% larval aqueous and alcoholic extracts. Data are expressed as the mean ± SEM. Samples were run in triplicate in three independent experiments, n = 5. Groups with different letters are significantly different at p < 0.05. * indicates high significance with respect to the control (p < 0.01).

Discussion
There is growing interest in exploring the roles of the insects' bioactive components, especially those with antioxidant and anticancer properties. Previous studies have only investigated the anticancer effect of Vespa sp. venom and its bioactive peptides [36][37][38]. However, the effect of the venom is not selective and can damage normal cells [39,40]. Aside from the venom, insect developmental stages (larvae and pupae) exhibit antioxidant and anticancer potential, with less toxicity for normal cells. Dutta et al. [32] reported an antioxidant effect for V. affinis aqueous pupal extract. Moreover, the larval hemolymph of M. domestica had antioxidant and cytotoxic properties against MCF7, but no cytotoxicity on normal Vero cells [11]. To the best of our knowledge, this is the first study to report that 5% V. orientalis larval extracts had an anticancer effect on MCF7 cells, and that this effect could be mediated by, at least in part, the induction of apoptosis, activation of antioxidants, and inhibition of migration and inflammation.
Previous studies have demonstrated cytotoxic effects for V. orientalis venom and attributed this effect to a bioactive peptide called mastoparan, which causes membrane destabilization and subsequent cell lysis [48,49]. Mastoparan also activates G-protein, which subsequently initiates mitochondrial permeability and apoptosis [50]. However, little is known about the effect of V. orientalis larval extracts on cancer cells. In the present study, we studied this effect and reported a notable dose-dependent antiproliferative potential for V. orientalis larval extracts on MCF7 with better influence for the aqueous extract which showed lower IC 50 (32.89 ± 1.84 µg/mL) than the alcoholic extract (61.48 ± 2.70 µg/mL). This indicates a more potent anticancer effect for the aqueous larval extract. Unlike most insect venoms, these extracts are less toxic on normal cells such as Vero cells, indicating a high safety margin. This is in agreement with the safe consumption of many Vespa Sp. by some tribes in India [31]. However, in vivo investigations on animal models of cancer are required to validate their biosafety and choose the optimal doses. Similar to V. orientalis venom and its ingredient mastoparan, the cytotoxic effect of larval extracts was mediated through induction of apoptosis as indicated by significant elevation of the three apoptotic genes Bax, caspase 3, and p53 and significant reduction of the anti-apoptotic Bcl2 gene with best apoptotic effect for the aqueous extract. Extracts used in the present study increased Bax, which induced the release of mitochondrial cytochrome c into the cytoplasm, further activating caspase3 expression [51]. Thus, these extracts could inhibit MCF7 proliferation via the initiation of the intrinsic pathway of apoptosis. However, the extrinsic apoptotic pathway cannot be overlooked. Therefore, further investigations on caspase 8 and Fas-L are needed. These results are in agreement with previous reports on the potent anticancer effect of M. domestica larvae extract and hemolymph on CT26 and MCF7 cancer cells [11,15] and C. albiceps larval extracts on a large variety of cancer cells including MCF7 [52].
Disrupting redox homeostasis is a central phenotype of several pathological states. Excessive oxidation harms different cellular components and is considered the hallmark for many diseases [53]. Reactive oxygen species (ROS) induce oxidative stress, which degrades proteins, lipids, and DNA [22,54,55]. The body prevents the over-release of free radicals and subsequently restricts oxidative stress through the activation of endogenous antioxidant enzymes (such as CAT, SOD, and GPx). Insects and their developmental stages (larvae and pupae) possess a powerful endogenous antioxidant system with a predominant effect for SOD [56,57]. Consistent with these findings, we also found that V. orientalis larval extracts possessed potent total antioxidant properties, as revealed by the DPPH assay. This effect was further proved on enzymatic and mRNA levels, and the results showed significant elevation in activities of antioxidant enzymes and expression of NrF2 and HO-1 genes following treatment with larval extracts. We also demonstrated a significant reduction in intracellular ROS as an indicator for oxidative stress. Similarly, Dutta et al. [32] found potent antioxidant properties for V. affinis aqueous pupal extract with higher SOD, CAT, and GST activities in human plasma (in vitro) and lower intracellular ROS in monocytes. El-Garawani et al. [11] also reported an antioxidant effect for M. domestica larval hemolymph with higher levels of SOD, TAC, and GSH and lower lipoperoxidation marker MDA levels. Several other studies also showed antioxidant effects for insect larval extracts such as blowflies [58] and beetles [59]. Interestingly, the increased anticancer potential of the aqueous extract was associated with a higher antioxidant status, which could be due to the existence of the defense constituents in larvae. In support, antioxidants can hinder tumorigenesis and growth and trigger apoptosis in cancer cells [11,23,60]. In general, the inhibitory effect on excessive ROS release and induction of antioxidant enzymes imply an advantageous role of V. orientalis larval extracts against oxidative stress-dependent diseases and cancer.
One of the main features of a potent anticancer drug is its ability to not only kill cancer cells, but also to prevent metastasis. Most mortality from cancer may be related to metastasis and the subsequent multiorgan dysfunction. Interestingly, V. orientalis larval extracts inhibited MCF7 metastasis as noticed by the results of both wound healing assay (which showed a humbled migration) and qPCR (reduced expression of the migrationrelated gene MMP9 and increased expression of the anti-migration gene TIMP1). Unlike apoptotic and antioxidant effects, which revealed a leading effect for aqueous extract, no significant difference was noticed between the two extracts with respect to their antimigration potential. Consistent with our results, cantharidin, a toxin extracted from beetles, also inhibited migration and invasion of human lung cancer cells through selective prevention of the expression of some MMP members [10].
The results obtained from qPCR revealed an anti-inflammatory effect for the larval extracts, as demonstrated by the downregulation of inflammation-related genes (NFκB and IL8), but with a predominant effect for the alcoholic extract. Using a similar mechanism, V. orientalis venom also possessed an anti-inflammatory effect [61]. Additionally, aqueous extracts of the house cricket, grasshopper, and silk moth contain notable anti-inflammatory and antioxidant activities [17,18]. Cancer cells induce inflammation via their ability to release inflammatory cytokines into the tumor microenvironment [62,63]. Inflammation plays an important role in cancer initiation and metastasis. When MCF7 is exposed to cytokines for long period, they undergo epithelial-mesenchymal transition, thereby facilitating cancer cell migration and metastasis. Hence, inhibition of cytokines such as NFκB hinders breast cancer cell migration [46,64]. Taken together, we provide V. orientalis larval extracts as novel inhibitors for chemotaxis-based migration of MCF7 cells.
HPLC analysis revealed the presence of several flavonoids and phenolic compounds, with the highest concentrations being for resveratrol and naringenin in the aqueous extract and rosmarinic acid in the alcoholic extract. In addition to other unknown higher peaks which worth further investigation. Thus, we suggest that the anticancer and antioxidant properties of these extracts could be attributed, at least in part, to these flavonoids and phenolic compounds. High levels of resveratrol and naringenin in the aqueous extract could explain its predominant anticancer and antioxidant potential relative to the alcoholic extract. Resveratrol has an anticancer effect and can potentiate the chemotherapeutic potential, reduce multidrug resistance, and limit metastasis when used in combination with standard anticancer drugs (reviewed in [65]). Resveratrol also has potent antioxidant properties that are mediated via activation of Nrf2 and its downstream target HO-1 in rat PC12 cells and normal human breast epithelial MCF10F cells [66]. It also induces the p53-dependent pathway in cancer cells [67]. Similarly, we also found that the resveratrolrich aqueous extract inhibited MCF7 proliferation, and this effect was accompanied by higher expression of Nrf2, HO-1, and p53. Naringenin inhibits the viability of a large variety of cancer cells and diminishes their migration and invasion [68]. It has been considered to be a perfect surgical adjuvant treatment for breast cancer patients, as it can modulate patient immunity and subsequently preventing tumor metastases after surgery [69]. Its anti-metastasis effect was associated with AKT pathway inhibition and downregulation of MMP2 and MMP9 [68]. On the other hand, the abundant amount of rosmarinic acid in the alcoholic extract could justify its superior anti-inflammatory effect. Rosmarinic acid possesses anti-inflammatory and anticancer properties against several cancer cell lines [70]. It can also cease the migration of MDA-MB-231BO human breast cancer cells from the breast to the bone through inhibition of NFκB ligand (RANKL), osteoprotegerin, and IL8 expression [71]. Due to its potent anti-inflammatory effect, rosmarinic acid has been used in the treatment of inflammatory diseases such as arthritis, colitis, rhinitis, and acute pancreatitis through inhibition of cytokines including NFκB, IL1b, and IL8 [72]. In parallel, we also reported that the rosmarinic acid enriched alcoholic extract minimized MCF7 growth, and this effect was associated with the downregulation of NFκB, and IL8.
This study provides new details regarding the anticancer effect of V. orientalis larval extracts against only one type of breast cancer cells (MCF7). However, it is worth confirming whether this effect also exists on other breast cancer cell lines to show that the observed effects are not only related to certain features in MCF7. Similarly, the effect of V. orientalis larval extract needs to be verified on animal models of breast cancer. Therefore, further in vitro and in vivo investigations are required to provide more mechanistic details in that regard.

Conclusions
Cancer cells maintain their viability via inhibition of apoptosis, initiation of inflammation, and migration. Stimulating apoptosis and targeting inflammation and migration can inhibit the proliferation of cancer cells and limit tumor progression. To the best of our knowledge, this is the first study to report that treatment with different V. orientalis larva extracts could inhibit MCF7 proliferation through induction of apoptosis, activation of antioxidant status, and inhibition of migration and inflammation. The aqueous extract showed the most potent anticancer effect, with a higher antioxidant effect but lower antiinflammatory properties than the alcoholic extract. Thus, V. orientalis larval extracts could be useful in the future as adjuvants for breast anticancer drugs and as antioxidant and anti-inflammatory agents in the treatment of oxidative stress and inflammation-related diseases. However, further in vivo preclinical studies are required to verify this effect in animal models and check whether these extracts could be clinically relevant and safe for patients.

Supplementary Materials:
The following are available online. Figure S1: Expression of Bax, caspase3 (Cas3), p53, and Bcl2 genes in Vero cells following treatment with 5% aqueous and alcoholic larval extracts as detected by qPCR. Data were normalized to the housekeeping gene (GAPDH) and expressed as the mean fold change ± SEM. Samples ran in triplicates in 3 independent experiments, n = 5.