Analysis of the Composition of Lyophilisates Obtained from Aloe arborescens Gel of Leaves of Different Ages from Controlled Crops

The aim of the study is to evaluate the composition of lyophilisates obtained from Aloe arborescens leaf gel at the age of one to four years. The leaves were obtained from controlled crops, which allowed to exclude environmental factors as variables. It was confirmed that the lyophilisates obtained from different years of Aloe arborescens leaf gel varied in chromatographic analyses in terms of aloin A and aloenin A content (high-performance liquid chromatography with diode-array detection HPLC-DAD, high-performance liquid chromatography with tandem mass spectrometric detection HPLC-MS/MS). Similarly, while testing the phenolic acids and the sum of polyphenols content, differences in their levels in leaf gel lyophilisates from plants of individual years were observed (spectrophotometric method UV-VIS). The lyophilisate composition analysis showed that the one-year-old leaves were characterized by the highest content of aloin A and aloenin A. While the content of polyphenols, including phenolic acids, was higher in the leaves of older plants. The antioxidant potential of the tested lyophilisates was assessed simultaneously. Regardless of the research model used (CUPRAC, DPPH, ABTS), an antioxidant effect was noted for Aloe arborescens leaves.


Introduction
Plant raw materials contain numerous groups of compounds with a biological effect. Especially the group of secondary metabolites in plant compounds show biological activity [1][2][3][4][5]. The synergism of the activity of selected metabolites shapes the final pharmacological effect of the plant material. There are many reports in the literature on the subject of the synergism of the action of various groups of secondary metabolites (e.g., Cannabis spp.) [6,7], also, of the concept of the so-called entourage, i.e., achieving better pharmacological parameters as a result of the presence of groups of secondary metabolites [8][9][10]. Secondary metabolites, as compounds produced as a defense reaction, respond to the variability of many environmental factors, which may modify the composition of secondary metabolites produced by the plant [11][12][13]. In the case of relatively constant external factors of perennial medicinal plant cultivation (example of controlled crops), the plant's age is an essential factor determining its secondary metabolite composition [14]. For almost every medicinal plant, it is important to obtain the raw material in the period of the optimal composition of secondary metabolites.
Aloe is a large genus of significant medical, cosmetic, and food importance, consisting of, according to Salehi et al., over 446 species belonging to the Xanthorrhoeaceae family, One of the effects associated with the presence of active compounds in Aloe arborescens is anticancer activity against liver cancer, resulting from the inhibitory effect on induction of preneoplastic glutathione S-transferase positive hepatocyte foci GST-P + − colon cancer (duodenal, intestinal cancer) [26]. Picchietti et al. confirmed the immunomodulatory effect of A. arborescens in bacterial and viral infections [25]. In comparison, Mogale et al. investigated the antidiabetic activity of aqueous extract of A. arborescens leaf gel based on the protection mechanism of pancreatic β-cells [27]. The antioxidant and antiinflammatory activity should also be pointed out in addition to the mentioned properties of Aloe arborescens [28]. Moreover, similarly to other species of the genus, Aloe arborescens is characterized as a strong laxative agent [29].
A comprehensive analysis of selected Aloe plants carried out by Zapata et al. enables the comparison of Aloe arborescens with other species of the genus. It is characterized by the highest total phenolic concentration and total antioxidant activity. This research team also conducted an analysis of the content of active compounds in Aloe arborescens leaves depending on the harvesting period-the comparison concerned leaves harvested in spring, summer, and winter. In all cases, the highest content was found for leaves harvested in summer [30].
So far, no relationship has been reported between the age of Aloe arborescens (one-, two-, three-, and four-year-old plants) and the biological properties of groups of compounds contained in leaves obtained from plants of subsequent years. Therefore, the aim of this study is to evaluate the composition of one-, two-, three-, and four-year-old Aloe arborescens leaves. Aloe arborescens leaves that were collected from controlled crops are used as the material for the study.

Results
Bearing in mind the need to standardize the raw material from Aloe spp., in our research, at first, the contents of aloin A and aloenin A were determined and compared in lyophilisates obtained from Aloe arborescens leaves of the years one to four. For this purpose, the high-performance liquid chromatography with diode-array detection (HPLC-DAD) method with the detection wavelength λ max = 295 nm and the mobile phase comprised of methanol and water in a gradient was developed and validated (Table S1, Supplementary Materials). Under the developed chromatographic separation conditions, the following were eluted: aloenin A (R T = 12.7 min) and aloin A (R T =16.5 min) (Figure 1).

Results
Bearing in mind the need to standardize the raw material from Aloe spp., in our research, at first, the contents of aloin A and aloenin A were determined and compared in lyophilisates obtained from Aloe arborescens leaves of the years one to four. For this purpose, the high-performance liquid chromatography with diode-array detection (HPLC-DAD) method with the detection wavelength λmax = 295 nm and the mobile phase comprised of methanol and water in a gradient was developed and validated (Table S1, Supplementary Materials). Under the developed chromatographic separation conditions, the following were eluted: aloenin A (RT = 12.7 min) and aloin A (RT =16.5 min) ( On the basis of the calibration curves for the reference substances, quantitative analysis was possible ( Figure 2). The aloin A content was highest in one-year-old leaves. Similarly, aloenin A also was found in the highest content in one-year-old leaves. It can be noticed that the content of aloin A and aloenin A decreased with the aging of the plant. On the basis of the calibration curves for the reference substances, quantitative analysis was possible ( Figure 2). The aloin A content was highest in one-year-old leaves. Similarly, aloenin A also was found in the highest content in one-year-old leaves. It can be noticed that the content of aloin A and aloenin A decreased with the aging of the plant.
(c) On the basis of the calibration curves for the reference substances, quantitative analysis was possible ( Figure 2). The aloin A content was highest in one-year-old leaves. Similarly, aloenin A also was found in the highest content in one-year-old leaves. It can be noticed that the content of aloin A and aloenin A decreased with the aging of the plant. Examples of such compounds include condensates with caffeic acid, whose characteristic base peak, visible in Figure 3, is at m/z 179. Differences in intensities of the discriminative ions may be caused by the aging of the plant.  As the next stage, the content of phenolic compounds in Aloe arborescens leaf lyophilisates obtained from controlled crops for one-, two-, three-, and four-year-old plants was assessed. Obtained results showed the highest content of polyphenols for three-year-old plants. In one-to three-year-olds, the content in the tested extracts increased, and for fouryear-old plants, the gel lyophilisates were lower than for three-year-old plants (Figure 4a). When it came to phenolic acids content analysis, lyophilisates from three-year-old leaves had by far the highest content of this group of compounds, and it was much higher com- As the next stage, the content of phenolic compounds in Aloe arborescens leaf lyophilisates obtained from controlled crops for one-, two-, three-, and four-year-old plants was assessed.
Obtained results showed the highest content of polyphenols for three-year-old plants. In one-to three-year-olds, the content in the tested extracts increased, and for four-year-old plants, the gel lyophilisates were lower than for three-year-old plants ( Figure 4a). When it came to phenolic acids content analysis, lyophilisates from three-year-old leaves had by far the highest content of this group of compounds, and it was much higher compared to leaves from other years ( Figure 4b).
As the next stage, the content of phenolic compounds in Aloe arborescens leaf lyophilisates obtained from controlled crops for one-, two-, three-, and four-year-old plants was assessed. Obtained results showed the highest content of polyphenols for three-year-old plants. In one-to three-year-olds, the content in the tested extracts increased, and for fouryear-old plants, the gel lyophilisates were lower than for three-year-old plants ( Figure 4a). When it came to phenolic acids content analysis, lyophilisates from three-year-old leaves had by far the highest content of this group of compounds, and it was much higher compared to leaves from other years (Figure 4b).
(a) (b) Figure 4. Concentration of (a) polyphenols and (b) phenolic acids in studied extracts.
All of the tested leaves showed antioxidant properties. During the CUPRAC assay, the determined IC50 values for the examined leaves were between 14.8-43.1 mg/mL. The lowest concentration needed to inhibit the complex of neocuproine's oxidizing properties and copper ion (II) exhibited in the ethanol extract prepared from the lyophilized gel from the leaves of the one-year-old plant. The IC50 values were higher for ethanol extracts of two-and three-year-olds, and the weakest antioxidative properties characterized the four- All of the tested leaves showed antioxidant properties. During the CUPRAC assay, the determined IC50 values for the examined leaves were between 14.8-43.1 mg/mL. The lowest concentration needed to inhibit the complex of neocuproine's oxidizing properties and copper ion (II) exhibited in the ethanol extract prepared from the lyophilized gel from the leaves of the one-year-old plant. The IC50 values were higher for ethanol extracts of two-and three-year-olds, and the weakest antioxidative properties characterized the four-year-old Aloe arborescens extract ( Figure 5). The aqueous extracts showed such weak antioxidant properties that it was impossible to determine the IC50 values for three-and four-year-old plants. Therefore the results are not included in the figure. year-old Aloe arborescens extract ( Figure 5). The aqueous extracts showed such weak antioxidant properties that it was impossible to determine the IC50 values for three-and fouryear-old plants. Therefore the results are not included in the figure. The results of the next applied antioxidant assay-DPPH method-indicated threeyear-old aloe leaves as the ones with the most potent antioxidant properties, slightly weaker were the properties of two-year-old plants, then four-year-old plants, and the weakest antioxidant properties in the test using the DPPH radical showed in the extracts obtained from one-year-old plants ( Figure 6). The results of the next applied antioxidant assay-DPPH method-indicated threeyear-old aloe leaves as the ones with the most potent antioxidant properties, slightly weaker were the properties of two-year-old plants, then four-year-old plants, and the weakest antioxidant properties in the test using the DPPH radical showed in the extracts obtained from one-year-old plants ( Figure 6). The results of the next applied antioxidant assay-DPPH method-indicated threeyear-old aloe leaves as the ones with the most potent antioxidant properties, slightly weaker were the properties of two-year-old plants, then four-year-old plants, and the weakest antioxidant properties in the test using the DPPH radical showed in the extracts obtained from one-year-old plants ( Figure 6).  In the study of the antioxidant properties using the ABTS radical, the most potent antioxidant properties were observed for the studied extract of two-year-old plants, weaker for three-and four-year-old, and the weakest for one-year-old plants (Figure 7).
In the study of the antioxidant properties using the ABTS radical, the most potent antioxidant properties were observed for the studied extract of two-year-old plants, weaker for three-and four-year-old, and the weakest for one-year-old plants (Figure 7). Statistical analysis of the results of antioxidant studies and investigations of phenolic content revealed statistically significant differences in all assays (Table S3, Supplementary Materials).

Discussion
Based on literature reports on the differences in the chemical composition of Aloe leaves depending on the harvesting season, A. arborescens leaves collected in late summer

Discussion
Based on literature reports on the differences in the chemical composition of Aloe leaves depending on the harvesting season, A. arborescens leaves collected in late summer were selected as the subject studied [30]. Zapata et al. conducted the research for various Aloe spp. (A. arborescens, A. aistata, A. claviflora, A. ferox, A. mitriformis, A. saponoaria, A. striata, A. vera), which resulted in information on the season's influence (spring, summer, winter) on the content of aloin, free putrescine, spermidine, and phenolic compound, and hydrophilic and lipophilic total antioxidant activity. For the species A. arborescens, the highest concentrations of tested compounds were noted for leaves harvested in summer [30].
Our research aimed to examine the relationship between the age of leaves (one-, two-, three-, four-year-old) of A. arborescens and the contents of specific compounds (aloin A, aloenin A, polyphenols, and phenolic acids) in gel lyophilisates. There are reports in the literature on the therapeutic multidirectional beneficial effects of the Aloe spp. on various body systems: gastrointestinal, respiratory tract, central and peripheral nervous system, skin, and eyes [16,31].
A. arborescens controlled greenhouse crops are an excellent model for comparing mainly the leaf content variation, due to the plants' age. Other variables such as soil, hydration, temperature, air humidity, and light exposure become eliminated and are considered constant parameters. As far as the harvesting cycle is concerned, in the case of Phytopharm Klęka crops, the age of the plant is counted from planting the seedling to the ground. In turn, as pharmaceutical raw material, the three-year-old and older leaves are used. In our research, for extract preparation, we used lyophilisates from one-, two-, three-, and four-year-old gel of leaves of A. arborescens. The use and storage of the lyophilisates avoided variations in the content of compounds, resulting from the easy evaporation of solvents commonly used in the preparation of extracts. Freeze-drying and extraction processes can be indicated as stages of storage and preparation of the raw material that does not change its quality.
As a result of the conducted research comparing the composition of lyophilisates obtained from the gel of the leaves of different ages, it was found, using the HPLC-DAD method, that the differences in the levels of aloin A were large and amounted to over 50% between individual years. The laxative effect based on hyperemia of pelvic organs may be associated with aloin A content, which in the large intestine under the influence of enzymes-glycosidases-releases the aglycone (aloe-emodin) [32]. Therefore, one-yearold leaves are considered the best plant material for the production of products with this indication.
The trend of the aloenin A level was similar-the highest in one-year-old plants. Aloenin A is not a compound found in all species of the genus Aloe. Its presence has been confirmed in 16 Aloe species out of 380 tested. The detection of aloenin has always been associated with the presence of aloin [33]. Taking into account the antioxidant properties of aloenin A and its potential in the stimulation of hair growth, as well as the beneficial effects of Aloe on the skin, one-year-old A. arborescens leaves seem to be the best candidate for testing the possibility of use in skin diseases from the studied years [34][35][36]. Additionally, due to the low-molecular structure, stability, and relatively high content of Aloe gel, aloenin A seems to be an excellent marker for the standardization of Aloe arborescens preparations [37].
All of the tested lyophilisates are characterized by antioxidant activity. The compounds present in the lyophilisates have the potential to neutralize free radicals. Polyphenols are biologically active compounds that neutralize free radicals by donating an electron or hydrogen proton, metal chelation, induction of antioxidant enzymes, and action as chain breaker of lipid peroxidation [38]. The polyphenols group includes various groups of compounds that contain a phenolic structure [39]. One of them is a group of phenolic acids. The properties of phenolic acids are well documented with respect to their protective health effects, such as antimicrobial, anticancer, anti-inflammatory, and anti-mutagenic properties [40].
Using the CUPRAC technique, the highest antioxidant potential for lyophilisates was obtained for one-year-old leaves. The analysis of the composition showed that in these lyophilisates, the levels of aloin A and aloenin A (4.18 and 10.31 mg/g d.m., respectively) are the highest, which is 2-3 times more than the lyophilisates obtained from the leaves of older plants. However, it showed the lowest quantity of polyphenols and phenolic acids, so it can be concluded that antioxidant activity presented by lyophilisates in CUPRAC assay is not affected by the presence of these groups of compounds. Therefore, in this case, the antioxidant property of extracts can be connected to the existence of other classes of compounds such as anthraquinones, e.g., aloin A, which are proven to be present in the extract or other groups which were not studied in our case. Moreover, there can be seen a relationship between the content of anthraquinones and CUPRAC assay results; with a decreasing amount of anthraquinones in the extract, there was a drop in the antioxidant potential of lyophilisates. This may suggest that anthraquinones, in the case of Aloe arborescens extracts, are responsible for the antioxidant effect by cupric ion reducing activity. This observation is in line with other studies, where it was confirmed that anthraquinones possess antioxidant activity via radical scavenging effect and ion reducing activity [41][42][43].
The potential to act against the oxidation process was also determined by the DPPH technique. The study showed that the lyophilisates obtained from three-year-old leaves have the highest antioxidant potential, which may be associated with a high content of polyphenols (354.88 mg/g d.m.) and phenolic acids (187.43 mg/g d.m.). Higher antioxidant potentials were obtained for ethanol extracts, and the difference was particularly large for extracts obtained from lyophilisates of four-year-old leaves.
The last technique for investigating the antioxidant potential was the ABTS method. As in the DPPH technique, ethanol extracts showed the greater potential, regardless of the leaves' age from which the gel to obtain the lyophilisates came. The highest antioxidant properties were characteristic for the lyophilisates for two-and three-year-old plants.
In this case, similar to the DPPH method, taking into account the content of phenolic compounds, it can be suggested that the antioxidant activity is also due to the presence of other compounds, e.g., a synergy between the action of polyphenols and anthranoid compounds can be suggested. Moreover, according to the literature on the subject, it can be seen that the choice of the alcoholic extraction solvent may affect the antioxidant activity of the extract-an example is the use of ethanol which causes the transition to the extract of more compounds with antioxidant activity potential [44,45].
Due to the potent antioxidant properties of polyphenols, including phenolic acids, it could be expected that their high content in three-year-old leaves would translate into their potentially highest antioxidant activity. However, this relationship can only be applied to the DPPH assay, where extracts from three-year-old leaves showed the best antioxidant activity, which can be connected to the highest amount of polyphenols and phenolic acids present in the raw material. This connection is especially seen in water extracts, where the one from three-year-old leaves revealed a higher quantity of polyphenols and a significantly higher amount of phenolic acids with respect to water extracts obtained from leaves in different ages. The methods used to test the extracts' antioxidant activity indicated that the one-, two-, and three-year-old leaves showed the highest activity, depending on the type of study (CUPRAC, DPPH, ABTS method, respectively). Therefore, it can be concluded that, besides phenolic acids, other compounds from the polyphenol group and other groups will affect the antioxidant properties.
The results of the study may indicate the beneficial usage of one-year-old and twoyear-old leaves of Aloe arborescens as a pharmaceutical material, since they are characterized by different quality and quantity properties, especially when it comes to anthranoid compounds composition. It could be advantageous to use the early age leaves as a material in the development of laxative agents.

Plant Material
Aloe arborescens Mill. fresh leaves supplied by Phytopharm Klęka S.A. (Klęka, Poland) were used for the study. The leaves were obtained from the one-, two-, three-, and fouryear-old plants growing in controlled crops (Figure 8). The plantation care treatment is limited to watering and weeding. As succulents, Aloe plants are watered moderately, using laboratory-controlled water. Fertilization is controlled, with mineral or organic fertilizers applied in minimal necessary amounts according to the needs and recommendations developed by relevant institutions, based on the results of analyses of soil samples. The risk of contamination is minimal as no pesticides, fungicides, herbicides, or fumigants are used during cultivation. Moreover, the type of plantation (monoculture), cultivation care treatments, and harvest also minimize risks of other contamination types.

Crop Conditions
Cultivation of Aloe arborescens Mill. was carried out in controlled, inbreed plantations in heated greenhouses. Depending on the season, temperature varies between 10 • C to 30 • C as plants do not withstand temperatures below 0 • C. During cultivation, ventilation and shading are applied in order to regulate temperature and humidity. Aloe arborescens is propagated vegetatively by shoots appearing in the lower part of the plant. Seedlings rooting takes place in pots using a peat substrate and lasts about a year. After that, plants are planted into the ground.
The plant material-fresh leaves-is collected manually by cutting off the whole plant; therefore, the harvest means the liquidation of the plantation lot. Since the cultivation engages lots of plants at different age stages, the harvest of plant material of certain age could be throughout the year. The age of the crop is counted from the time of planting seedlings in the ground.
The plantation care treatment is limited to watering and weeding. As succulents, Aloe plants are watered moderately, using laboratory-controlled water. Fertilization is controlled, with mineral or organic fertilizers applied in minimal necessary amounts according to the needs and recommendations developed by relevant institutions, based on the results of analyses of soil samples. The risk of contamination is minimal as no pesticides, fungicides, herbicides, or fumigants are used during cultivation. Moreover, the type of plantation (monoculture), cultivation care treatments, and harvest also minimize risks of other contamination types.

Lyophilization
A pulp was obtained from the leaves by leaf skin separation. It was stored at −20 • C in a freezer for 24 h, and then frozen pulp was lyophilized using a freeze-dryer HetoPowerDry PL3000 Freeze Dryer (Thermo Fisher Scientific, Waltham, MA, USA). The process lasted for 48 h at a temperature of −50 • C under vacuum conditions. Lyophilisates were stored in the conditions of the minimalized humidity and light access.

Extracts Preparation
The extracts were prepared from the lyophilized leaf gel of particular age plants. Then, 100 mg of the lyophilized milled gel were extracted with ethanol 96% (v/v) or water with the use of ultrasounds. After 30 min, the supernatants were decanted, and the lyophilisates were extracted twice more under the mentioned conditions. Finally, the supernatants collected for each lyophilisate were mixed together, filtered through syringe filters, and concentrated to dryness by applying rotary evaporation. The residues were dissolved in 1 mL of ethanol 96% (v/v) or water to obtain a 100 mg/mL concentration.

Preparation of the Standard Solutions for HPLC Analyses
Two substances were used as standards-aloin A, aloenin A. Their presence in the studied extract was confirmed using mentioned gradient HPLC-DAD method. After setting a baseline, injections of 20 µL volume of extracts were made. Each compound's identity was confirmed by comparing the retention times of the obtained peaks with the standards. For the purpose of quantitative research, calibration curves were prepared.

Preparation of Extract Solutions for HPLC Analyses
Stock extract (100 mg of lyophilisate in 1 mL of ethanol) was filtered through 0.2 µm syringe filters. Then, 50 µL of the filtered extract was diluted with ethanol up to a 1 mL volume, and the prepared solution was injected. The concentrations of standard compounds were calculated on the basis of the obtained HPLC chromatograms and standard curves.

HPLC Analysis
The RP-HPLC-DAD method was applied to confirm the identity of the material. For this purpose, two standard substances were used as standards-aloin A and aloenin A. In the study, the HPLC Shimadzu Prominence-i LC-2030C equipped with DAD detector was used (Shimadzu, Tokyo, Japan). The software was LabSolution DB/CS version 6.50 (Shimadzu, Tokyo, Japan). The stationary phase was LiChrospher 100 RP-18e (250 mm × 4.6 mm, 5 µm) HPLC column (Merck, Warsaw, Poland), the mobile phase comprised of methanol (phase A) and water (phase B) in a gradient (Table S2,  The method is based on a color reaction of the Folin-Ciocâlteu reagent with the contained phenolic groups in the presence of sodium carbonate. Therefore, 1 mL of the extract was vortexed with 1 mL of Folin-Ciocâlteu reagent for 3 s, then 1 mL of sodium carbonate and 7 mL of water were added and mixed again by vortex for 3 s. After 90 min of incubation in the dark, the resulting colored complex was determined spectrophotometrically at wavelength 765 nm in the Metertech SP-830 spectrophotometer (Metertech, Taipei, Taiwan). In order to determine the content of compounds reactive with the Folin-Ciocâlteu reagent, a standard curve for gallic acid was established. Then, using the determined curve, it was converted to the content in 1 g of the dry mass of the extract.

Phenolic Acids
The presence of phenolic acid compounds was determined by the colorimetric method described in Polish Pharmacopoeia VI [46]. The study was performed by adding to 5 mL of distilled water in a volumetric flask, analyzed extract, 0.5 M solution of hydrochloric acid, Arnov's reagent, and sodium hydroxide in a volume of 1 mL each. After diluting with distilled water to 10 mL volume, spectroscopy measurement was performed at the wavelength of 490 nm.
The content of phenolic acids was converted to caffeic acid on the basis of on the drawn standard curve and expressed in mg per 1 g of dry mass.

Antioxidant Activity Determination
The extract's antioxidant properties were determined by using CUPRAC (cupric ion reducing antioxidant capacity) assay, which was performed according to Apak et al. with modifications [47]. The study is based on the reduction of the complex of neocuproine and cupric ion (II), which results in the change of the sample's color from bluish to yellow. The CUPRAC reagent consists of equal volumes of 7.5 mM ethanolic 96% (v/v) solution of neocuproine, 10 mM solution of copper chloride (II), and ammonium acetate buffer of Ph = 7.0. The assay was performed by mixing 50 µL of aloe leaf gel extract with 150 µL of CUPRAC reagent. The solution was shaken and incubated in the dark at room temperature for 30 min. After the incubation, absorbance was measured at the wavelength of λ = 450 nm at Multiskan GO plate reader (Thermo Fisher Scientific, Waltham, MA, USA). As a standard, vitamin C was used. The results were presented as the IC 50 (inhibitory concentration), which means the minimum concentration of the extract, which reduces the initial, oxidized form of the complex by 50%.

DPPH Method
Determination of activity against DPPH radicals was performed on the basis of the method of Hatano et al. with Amarowicz modifications [48]. The principle of the method was based on the spectrophotometric (Metertech SP-830, Taipei, Taiwan) measurement of the color of the reaction mixture, in which, depending on the antioxidant capacity of the tested extract, free radicals DPPH (1,1-diphenyl-2-pyrrylhydrazyl) were swept away. The absorbance measurement was made at 517 nm after incubation for 30 min at room temperature and protected from light. The calculated percentage of the sweep was put into the standard curve for the Trolox.

ABTS Method
Determination of activity against ABTS cation radicals was performed according to the method described by Re et al. [49]. The test solution was introduced into the reaction medium containing the previously generated cation radical ABTS [2,2 -azinobis (3-ethylbenzothiazoline-6-sulfonate)]. The presence of antioxidants caused the reaction mixture to change color. At the same time, in order to prepare the calibration curve, the absorbance was measured in parallel at the same wavelength of 735 nm of samples containing appropriate concentrations of the reference substance-Trolox, and the results were expressed in its equivalents.

Statistical Analysis
The results were analyzed using Statistica software (version 13.0, Stat Soft Inc., Tulsa, OK, USA). An ANOVA test was used to compare results of the measurements in the studied extracts obtained from the plant at different age. The differences were considered to be significant when p ≤ 0.05.

Conclusions
As a result of the conducted research, we proved that the differences in the content of anthranoid compounds and phenyl pyrone derivatives-aloin A and aloenin A-are significant in lyophilisates obtained from Aloe arborescens leaves of different ages (oneto four-year-old). The developed chromatographic method (HPLC-DAD) can be recommended as suitable for the standardization of lyophilisates for the content of aloin A and aloenin A. The spectrophotometric determination of the sum of polyphenols and phenolic acids revealed the differences in the content of these groups of compounds in lyophilisates obtained from leaves of different ages.
All tested lyophilisates exhibited an antioxidant effect, which is worth emphasizing; the technique for determining the potential should depend on the group of secondary metabolites for which we want to monitor the antioxidant effect.
Supplementary Materials: The following are available online, Table S1. Validation parameters of HPLC-DAD method, Figure S1. Mass spectra for aloin A, Figure S2. Mass spectra for aloenin A, Table  S2. HPLC-DAD method gradient. Mobile phase consists of methanol-Phase A and water-Phase B, Table S3. The results of statistical analysis (ANOVA test).