Trichilones A–E: New Limonoids from Trichilia adolfi

In addition to the trichilianones A–D recently reported from Trichilia adolfi, a continuing investigation of the chemical constituents of the ethanol extract of the bark of this medicinal plant yielded the five new limonoids 1–5. They are characterized by having four fused rings and are new examples of prieurianin-type limonoids, having a ε-lactone which in 4 and 5 is α, β- unsaturated. The structures of the isolated metabolites were determined by high field NMR spectroscopy and HR mass spectrometry. The new metabolites were shown to have the ε-lactone fused with a tetrahydrofuran ring which is connected to an oxidized hexane ring joined with a cyclo-pentanone having a 3-furanyl substituent. As the crude extract possesses antileishmanial activity, the compounds were assayed for cytotoxic and antiparasitic activities in vitro in murine macrophage cells (raw 264.7 cells) and in Leishmania amazoniensis as well as L. braziliensis promastigotes. Metabolites 1–3 and 5 showed moderate cytotoxicity (between 30–94 µg/mL) but are not responsible for the antileishmanial effect of the extract.


Introduction
The Meliaceae family of plants includes species dispersed in tropical areas of America, Africa and Asia [1,2]. The Trichilia genus, part of this family, comprises several species that have been studied as they are used in traditional medicine, and phytochemical investigations have revealed that limonoids are typical constituents. The limonoids are tetranortriterpenoids, based on the triterpene class, which have been reported from several species of not only the Meliaceae but also the Cucurbitaceae and Rutaceae families. They are present, for example, in sweet-or sour-scented citrus fruit. Limonoids are characterized for having various carbon skeleta, being highly oxygenated and often substituted with a 3-furanyl moiety [3]. From Trichilia, different types of limonoids have been reported including azadirachtin and the trichilins (trichilin type, e.g., trichilin A from T. roka) [4,5], the trichisintons (phragmalines type, e.g., trichisinton A from T. sinensis) [6], the trichinenlides (mexicanolide type, e.g., trichinenlide A from T. sinensis) [7], trichavensin (prieurianin type, from T. havannensis) [8], hirtin (cedrelone type, from T. americana) [9] and protolimonoids (e.g., from T. elegans) [10]. A few structures are shown in Figure 1, and it is obvious that the limonoids can be structurally varied in an unlimited way. The compounds isolated in this investigation are shown in Figure 2.
Trichilia species are frequently used in traditional medicine. Bark, leaves, fruits and roots have been used to treat a broad variety of disorders, including stomach, diarrhea, dermatitis, taeniasis, pneumonia, lung, kidney and liver pains, as well to heal wounds [11]. The biological and pharmacological properties of Trichilia species have been reviewed by Longhini et al. [1], Garg [12], Curcino Vieira et al. [2] and Komane et al. [13]. More recently, the hortiolide-type limonoids, with five fused rings. An ε-lactone is attached to hydrofuran ring that is connected to a bicyclo [5.1.0] hexane system, joined wi clopentanone containing a 3-furanyl substituent. Continuing our investigation constituents of T. adolfi, with the aim to characterize its antiparasitic metabol present study is focused on the characterization of new metabolites present in t The structures of the isolated metabolites were elucidated by spectroscopic a HR-MS, and 1D and 2D NMR experiments (COSY, HMBC and NOESY correlat summarized in . The cytotoxicity of pure metabolites was evaluat vitro cell cultures of murine macrophages (Raw 264.7 cells), while the leishm properties were evaluated in the Leishmania amazoniensis and L. braziliensis proma   In a recent study by us, the four novel limonoids trichilianone A-D were isolated from the bark of Trichilia adolfi [21]. The structures of the trichilianones appear related to the hortiolide-type limonoids, with five fused rings. An ε-lactone is attached to a tetrahydrofuran ring that is connected to a bicyclo [5.1.0] hexane system, joined with a cyclopentanone containing a 3-furanyl substituent. Continuing our investigation of the constituents of T. adolfi, with the aim to characterize its antiparasitic metabolites, the present study is focused on the characterization of new metabolites present in the bark. The structures of the isolated metabolites were elucidated by spectroscopic analysis; HR-MS, and 1D and 2D NMR experiments (COSY, HMBC and NOESY correlations are summarized in . The cytotoxicity of pure metabolites was evaluated in in vitro cell cultures of murine macrophages (Raw 264.7 cells), while the leishmanicidal properties were evaluated in the Leishmania amazoniensis and L. braziliensis promastigotes.

Structure Determination of Trichilone A (1)
Trichilone A, (1, name proposed by us) was isolated as a white amorphous powder The elemental composition of 1 was determined by LC-HRMS experiments, the [M + H] ion was observed at m/z = 617.2963 which corresponds to C33H45O11 (calculated 617.2962 The elemental composition C33H44O11 reveals that the unsaturation index of 1 is 12. The 1 H and 13 C 1D NMR spectra display signals for exactly 44 protons and 33 carbons, and ac cording to their chemical shifts the structure of 1 should contain 5 carbonyls and two C-C double bonds suggesting that 1 has five rings. In addition, the 1D NMR data indicate th presence of one methoxy group, five oxygenated but saturated carbons, fou non-protonated but saturated carbons, eight saturated CH-groups (of which three ar oxygenated), three saturated methylene groups and nine methyl groups. The atom numbering used in this study (see Figure 2) follows the same system as in our previou work [21], which is based on an earlier study of hortiolide-type limonoids isolated from Hortia colombiana [22].
A careful analysis of the HMBC data gives critical information about the positions o the methyl groups as well as the core structure of 1. The two methyl groups 24-H3 and 25-H3 are both singlets and both give strong HMBC correlations to C-4 and C-5, and t The elemental composition C 33 H 44 O 11 reveals that the unsaturation index of 1 is 12. The 1 H and 13 C 1D NMR spectra display signals for exactly 44 protons and 33 carbons, and according to their chemical shifts the structure of 1 should contain 5 carbonyls and two C-C double bonds suggesting that 1 has five rings. In addition, the 1D NMR data indicate the presence of one methoxy group, five oxygenated but saturated carbons, four non-protonated but saturated carbons, eight saturated CH-groups (of which three are oxygenated), three saturated methylene groups and nine methyl groups. The atom numbering used in this study (see Figure 2) follows the same system as in our previous work [21], which is based on an earlier study of hortiolide-type limonoids isolated from Hortia colombiana [22].
A careful analysis of the HMBC data gives critical information about the positions of the methyl groups as well as the core structure of 1. The two methyl groups 24-H 3 and 25-H 3 are both singlets and both give strong HMBC correlations to C-4 and C-5, and to each other's carbons. 24-H 3 and 25-H 3 are consequently geminal and attached to the non-protonated C-4, which according to its chemical shift is oxygenated but also connected to C-5. The singlet 19-H 3 gives four HMBC correlations, to C-1, C-5, C-9 and C-10, and as C-10 is the only non-protonated carbon of these four, the 19-H 3 methyl group is attached to this carbon. This reveals the bond between C-5 and C-10. The singlet 26-H 3 gives only three HMBC correlations, to C-8, C-9 and C-14. The non-protonated C-8 displays a chemical shift that suggests it to be oxygenated, and it is consequently to this carbon that the 26-H 3 methyl group is attached. Finally, the methyl 18-H 3 , which is also a singlet, gives HMBC correlations to C-12, C-13, C-14 and C-17, suggesting that is attached to the non-protonated C-13. This reveals the following partial carbon skeleton: C-24/25-C-4-C-5-C-10-C-9-C-8-C-14-C-13-C-18. Strong 1 H-1 H couplings between 9-H and 11-H (8.0 Hz) and between 11-H and 12-H (11.6 Hz), along with HMBC correlations from 9-H to C-8, C-10 and C-11, from 11-H to C-9 and C-12, and from 12-H to C-11 and C-13, closes the first ring. In this, the chemical shifts for 11-H/C-11 and 12-H/C-12 suggest that these two carbons are oxygenated, more specifically acylated. This is confirmed by the HMBC correlations from 11-H, 2"-H, 3"-H 3 and 4"-H 3 to C-1", and internal COSY and HMBC correlations between 2"-H, 3"-H 3 and 4"-H 3 and their corresponding carbons demonstrate the presence of an isobutyric acid ester at C-11. 12-H. The methyl singlet 2 -H 3 give HMBC correlations to C-1 , showing that C-12 has an acetoxy substituent. On the other side of C-13 we find C-17 (with one proton) as discussed above, which is connected to C-16 (with two protons) according to the 1 H-1 H couplings between 17-H and 16-Ha (8.7 Hz) and between 17-H and 16-Hb (11.1 Hz). This is confirmed by the HMBC correlations from 17-H to C-16, and between 16-H 2 to C-17. 14-H and 16-H 2 give HMBC correlations to the keto function, while 14-H in addition gives correlations to C-8, C-12, C-13, C-17 and C-18, which show the position and nature of C-15 and close the second ring. The missing substituent on C-17 is a 3-furanyl group, and this partial structure was determined in the same way as with the trichilianones [21] by COSY, HMBC and 1 J(C,H) couplings constants. This forms the third ring accounting by itself for three unsaturations, and the NMR data are quite similar to those reported for other limonoids containing a 3-furanyl substituent [23]. COSY and HMBC correlations between 5-H and 6-H 2 , and these protons and C-6 and C-7, and the HMBC correlation of the methoxy protons to C-7 show that this carbon is not part of a lactone. We have already noted that C-1 is connected to C-10, and the COSY correlation between 1-H and 2-H 2 in addition to the HMBC correlations between 1-H and C-3 and C-3 and between 2-H 2 and C-1, C-3 and C-10 demonstrate this partial structure. Evidence that C-3 is part of an ε-lactone [24], the fourth ring, is shown by the weak but clear HMBC correlation between 24-H 3 and C-3. All protons and carbons are now accounted for, remaining one oxygen and the formation of a fifth ring. As it has been determined that both C-1 and C-8 are oxygenated, the formation of a tetrahydrofuran ring with the last oxygen bond to these both carbons is the only way to finalize the structure elucidation. A similar ring system has been identified in limonoids from species belonging to the genus Hortia [25].
The relative stereostructure of 1 was determined by analyzing its NOESY 2D spectra. No NOESY correlation was observed between 18-H 3 and 12-H, 14-H or 17-H, suggesting that the latter three protons are positioned on the opposite side compared to the methyl group (which would be α if the stereostructure in Figure 2 is absolute). This was confirmed by the correlations observed between 12-H and 17-H, and between both these protons and 14-H (all three β). However  Figure 2. The fact that 9-H, C-19 and C-26 are on the same side of the molecule, would bend the molecule along C-8/C-9 and explain the weak NOESY correlations observed between 12-H, 14-H, 5-H and 6-H 2 , even if they on paper appear to be too far apart. The corresponding configuration has also been suggested in hortiolide-type limonoids [25].

Structure Determination of Trichilone B (2)
Trichilone B, (2) was isolated as a white amorphous powder. 2 has the elemental composition C 34 H 46 O 11 , deduced by LC-HRMS experiments in which the molecular ion was observed at m/z = 631.3120 [M + H] + (calculated for C 34 H 47 O 11 631.3118). Consequently, the unsaturation index of 2 is 12, as in 1, but having an additional carbon and two hydrogens. NMR 1D 1 H and 13 C NMR data show many similarities with those of 1, and it appears likely that 2 shares its major structure with 1. A comparison of the NMR data show that 2 has an additional methylene group, and as 2 is missing the signals for the isobutyric acid ester of 1, it appears that 2 has been replaced with a 2-methyl butyric acid ester. This was confirmed by the HMBC correlations of the surrounding carbons. Methyl 5"-H 3 , a doublet, correlate with C-1", C-2" and C-3", while the protons of 4"-H 3 , a triplet, correlate with C-2" and C-3". The corresponding COSY correlations were also observed. 11-H gives a HMBC correlation to C-1", placing this acyloxy group at C-11. The relative stereochemistry was determined by NOESY experiment. As with 1, no NOESY correlations was observed between 18-H 3 and 12-H, 14 Figure 2 and reveals that 2 has the same relative stereochemistry as 1.

Structure Determination of Trichilone C (3)
Trichilone C, (3) 3 has one extra oxygen, and examination of the NMR 1D 1 H and 13 C NMR data suggests that a saturated methine on 2 has been hydroxylated in 3. The signals for 45 protons can be observed in the proton spectrum, supporting the suggestion that a hydroxyl group with an exchangeable proton has been introduced. Seven of the 12 unsaturations are accounted for five carbonyl groups and two C-C double bonds, so 3 has consequently five rings just as 1 and 2.
Most of the COSY and HMBC correlations discussed above for 1 and 2 can also be observed in the 2D spectra of 3, suggesting that 3 has a similar structure. A major difference is the transformation of the 11-H from a double doublet to a doublet in the proton spectrum. 11-H only couples with 12-H in 3, not to 9-H, and it appears as if C-9 is the carbon that has been hydroxylated, as a HMQC experiment revealed that C-9 is no longer protonated. By examining the correlations in the HMBC spectrum, it is evident that the protons in the vicinity of C-9 (1-H, 11-H, 19-H 3 and 26-H 3 ) correlate with a new carbon resonance at 89.2 ppm, which consequently is the hydroxylated C-9. When assigning the relative configuration of 3 using the data from NOESY experiments, the corresponding correlations as discussed above were noted. This includes correlations from 26-H 3 to 1-H and 11-H, from 12-H to 14-H and 17-H, and from 19-H 3 to 11-H, 6-H 2 and 2-Hb. This suggest that the configuration of C-9 is the same as in 1/2, and this is confirmed by the NOESY correlations from 12-H and 14-H to 5-H, caused by the folding of the molecule (also discussed above).

Structure Determination of Trichilone D (4)
Trichilone D, (4) was obtained as a white amorphous powder. The elemental composition of 4 is C 31 H 38 O 11 , which was determined by LC-HMRS experiments in which the molecular ion peak was observed at m/z = 587.2484 [M + H] + (calculated for C 31 H 39 O 11 : 587.2492). The saturation index for 4 was consequently 13, one more than the previously discussed trichilones, and the examination of the NMR 1D 1 H and 13 C NMR spectra reveals the presence of five carbonyl groups and three C-C double bonds. This suggests that 4 consists of five rings. The 13 C NMR spectra show a presence of ten methine carbons (including four olefinic and two oxygenated), two methylene groups, ten non-protonated carbons (including five carbonyl and three oxygenated carbons) and eight methyl groups (including a methoxy group). In 4, C-9 is no longer hydroxylated as it demonstrates proton coupling between 9-H and 11-H. A major difference between 4, and for example 1, is the HMBC correlations observed from 19-H 3 , besides the expected correlations to C-5, C-9 and C-10, a correlation is also observed to an unsaturated carbon with the chemical shift 177.3 ppm. This should be C-1, and as also C-2 is unsaturated (with the carbon shift 95.1 ppm) in 4, a main difference has been the introduction of a carbon-carbon double bond between C-1 and C-2 in the ε-lactone ring in 4. The carbon chemical shifts of C-1 and C-2 of 4 are unusual, but this is caused by the double electronic effect of an electron withdrawing carbonyl group at C-3, making it α,β-unsaturated, and the oxygenation of C-1. Consequently, the 1 J(C,H) coupling constants of C,H-2 is large (167 Hz). In addition, both C-11 and C-12 are acetoxylated as shown by the HMBC correlations from both 12-H and 2 -H 3 to C-1 , and from both 11-H and 2"-H 3 to C-1". NOESY experiment determines that 4 conserve the same relative configuration as 1.

Structure Determination of Trichilone E (5)
Trichilone E, (5) was isolated as a white amorphous powder. The elemental composition of 5 is C 33 H 42 O 11 , as deduced by LC-HMRS experiments in which the molecular ion peak was observed at m/z = 615.2798 [M + H] + (calculated for C 33 H 43 O 11 ; 615.2805) in positive-ion mode. 5 consequently contains 2 carbons and 4 hydrogens more than 4 but retains an unsaturation index of 13. As with 1 and 2, the 1D NMR data of 4 and 5 are very similar (see Tables 1 and 2). One of the acetyl groups of the former is obviously missing, and the signals for an isopropyl group have been added in the spectra of the latter. The HMBC correlations observed between 2"-H, 3"-H 3 , 4"-H 3 and 11-H and C-1" demonstrate that the acetyl group at C-11 in 4 has been exchanged for an isobutyric acid ester in 5. NOESY experiments show that the relative configuration of 5 is the same as in 4.

Biogenesis of the Trichilones
Not much is known about the biogenesis of this type of compounds, nor of the mechanisms of reactions involved in the biosynthesis of these highly complex molecules. Nevertheless, a suggestion for the biogenesis of 1-5 based on studies of similar compounds [26] is shown in Figure 6. The limonoids have a triterpene origin, and the intermediate 6 (an azadirone-type metabolite) can be imagined as the precursor (Figure 6). An oxidation of the C-7/C-8 bond to a lactone, followed by hydrolysis and a 180 • bond rotation around C-9/C-10 would make a cyclization to form a tetrahydrofuran ring possible. A second oxidation of the C-3/C-4 bond could produce the ε-lactone ring and thereby the five-ring system. However, this should only be regarded as a possibility.    Table 3 summarizes the biological activities of the compounds isolated. The results shown that these compounds are not responsible for the activity observed for the crude extract (up to 50 μg/mL) on Leishmania amazoniensis and L. braziliensis promastigotes in vitro. A moderate cytotoxicity in murine macrophage cells was noted (IC50 values between 30 and 94 μg/mL), which is higher compared with the positive control used in the assay (miltefosine, 21 μg/mL).

Compound
L.a L.b Raw Figure 6. A possible biogenesis of compounds 1-5. Table 3 summarizes the biological activities of the compounds isolated. The results shown that these compounds are not responsible for the activity observed for the crude extract (up to 50 µg/mL) on Leishmania amazoniensis and L. braziliensis promastigotes in vitro. A moderate cytotoxicity in murine macrophage cells was noted (IC 50 values between 30 and 94 µg/mL), which is higher compared with the positive control used in the assay (miltefosine, 21 µg/mL).

Extraction and Isolation
Milled dried bark (1.0 kg) were macerated with 5.0 L ethanol for 72 h at room temperature to provide a crude extract after evaporation of the solvent (22.0 g, 2.2% of bark weight). This was then re-suspended in aqueous methanol (H 2 O: MeOH, 80:20, 0.5 L) and extracted three times with 0.5 L n-heptane, yielding 3.1 g (14% of the crude extract) after evaporation of the solvent. This was followed by the extraction of the aqueous methanol phase with CHCl 3 (0.5 L), yielding 6.4 g (29% of the crude extract) after evaporation of the solvent, whereafter the aqueous phase was freeze-dried to yield 12.5 g (57% of the crude extract). The CHCl 3 fraction was subjected to SiO 2 gel column chromatography (1:30, w/w) eluted with mixtures of n-heptane: EtOAc (from 8:1 to 2:8 v/v). TLC analysis provided four main fractions from the least polar to the most polar: F1 (2.5 g), F2 (0.27 g), F3 (0.77 g) and F4 (0.68 g). Further fractionations were performed with fractions F1, F2 and F3 using a semi-preparative HPLC (vide supra for details) and eluted with mixtures of solvent A (3 mM formic acid in water) and solvent B (acetonitrile). The elution was performed in gradient mode, starting with A:B 85:15, reduced linearly to 77:23 in 5 min, a second linear decrease to 74:26 in 25 min, a third linear decrease to 60:40 in 30 min, and finishing with 54:46 for 45 min. All samples were filtered through 0.25 µm membrane filter prior to HPLC fractionations. From F2, crude Trichilone B (2) (5.1 mg, Rt = 15.35 min, 0.023% of raw extract) was obtained after SiO 2 column chromatography eluting with n-heptane: EtOAc 8:2 (v/v, isocratic) and further purification by the HPLC procedure. From F3, Trichilone A (1) was isolated after SiO 2 column chromatography of F3 eluting with n-heptane: EtOAc 6:4 (v/v, isocratic) to obtain a fraction that yielded pure 1 after HPLC purification (3.0 mg, Rt = 12.3 min, 0.013% of the raw extract). In the same fraction Trichilone C (3) was purified after HPLC isolation (6.3 mg, Rt = 18.3 min, 0.028% of raw extract). Finally, F4 was subjected to HPLC purification afforded Trichilone D (4) (12.0 mg, Rt = 15.8 min, 0.054% of raw extract) and Trichilone E (5) (14.6 mg, Rt = 18.0 min, 0.066% of raw extract).

Cytotoxicity
The Raw 264.7 murine macrophage cell line was purchased from the American Type Culture Collection (ATCC-TIB71) (Manassas, VA, USA). The cells were maintained in DMEM-HG medium supplemented with 10% fetal bovine serum, 100 U/mL of penicillin and 100 µg/mL of streptomycin and sodium bicarbonate (2.2 g/L) in humidified atmosphere at 37 • C with 5% CO 2 . Samples were prepared as described above and added (in 100 µL DMSO) at different concentrations (6.2-200 µg/mL). Medium blank, control drugs and cell growth controls were included to evaluate cell viability. The plates were incubated for 72 h at 37 • C with 5% CO 2 . After incubation for the indicated time, the cells were washed, after which 10 µL of Resazurin reagent (2.0 mM) was added. They were further incubated at 37 • C for 3 h in a humidified incubator. The IC 50 values were assessed using a fluorometric reader (540 nm excitation, 590 nm emission) and Gen5 software. All assays were performed in triplicate.

Conclusions
The isolation and structure elucidation of five new limonoids from Trichilia adolfi was reported, and to our disappointment they were not found to possess antileishmanial activity. Not much is known about the biogenesis of this type of these highly complex molecules. Nevertheless, a suggestion for the biogenesis of 1-5 is shown in Figure 6. The limonoids have a triterpene origin, and the intermediate 6 (an azadirone-type metabolite) can be imagined as a precursor ( Figure 6). An oxidation of the C-7/C-8 bond to a lactone followed by hydrolysis and a 180 • bond rotation around C-9/C-10, may facilitate the formation of a tetrahydrofuran ring. A second oxidation of C-3/C-4 would produce the ε-lactone ring and thereby the correct five-ring system. Manipulation of the two carboncarbon double bonds (C-1/C-2 and C-9/C-11) could then yield all five metabolites isolated in this investigation. Institutional Review Board Statement: Not applicable, as the study did not involve humans or animals.

Informed Consent Statement:
Not applicable, as the study did not involve humans or animals. Data Availability Statement: All data are available in this publication and in the Supplementary Materials.