A Review on Recent Advances in Nitrogen-Containing Molecules and Their Biological Applications.

The analogs of nitrogen-based heterocycles occupy an exclusive position as a valuable source of therapeutic agents in medicinal chemistry. More than 75% of drugs approved by the FDA and currently available in the market are nitrogen-containing heterocyclic moieties. In the forthcoming decade, a much greater share of new nitrogen-based pharmaceuticals is anticipated. Many new nitrogen-based heterocycles have been designed. The number of novel N-heterocyclic moieties with significant physiological properties and promising applications in medicinal chemistry is ever-growing. In this review, we consolidate the recent advances on novel nitrogen-containing heterocycles and their distinct biological activities, reported over the past one year (2019 to early 2020). This review highlights the trends in the use of nitrogen-based moieties in drug design and the development of different potent and competent candidates against various diseases.


Introduction
Nitrogen-based heterocyclic chemistry is an important and unique class among the applied branches of organic chemistry, with a significant amount of research dedicated to the development of novel molecules and composites. These molecules have received increasing attention over the past two decades. They contributed to the development of numerous organic synthesis protocols and found abundant applications in the chemical sciences [1][2][3][4][5]. Many N-heterocyclic compounds that are broadly distributed in Nature, possess physiological and pharmacological properties and are constituents of many biologically important molecules, including many vitamins, nucleic acids, pharmaceuticals, antibiotics, dyes and agrochemicals, amongst many others [6][7][8][9][10]. Moreover, they form an integral part of many pharmacologically active molecules. The base pairs of DNA and RNA (guanine, cytosine, adenine, and thymine) are also made up of N-heterocyclic compounds, namely purines, pyrimidines, etc. These nitrogen-containing heterocyclic molecules with distinct characteristics and applications have gained prominence in the rapidly expanding fields of organic and medicinal chemistry and the pharmaceutical industry [11][12][13]. Furthermore, the electron-rich nitrogen heterocycle is not only able to readily accept or donate a proton, but it can also easily establish diverse weak interactions. Some of these intermolecular forces, such as like hydrogen bonding formation, dipole-dipole interactions, hydrophobic effects, van der Waals forces and π-stacking interactions of nitrogen compounds have increased their importance in the field of medicinal chemistry and allows them to bind with a variety of enzymes and receptors in biological targets with high affinity due to their improved solubility. The structural features of their derivatives are beneficial sice they exhibit broad bioactivities.
A glance at the FDA databases reveals the structural significance of nitrogen-based heterocycles in drug design and engineering of pharmaceuticals. Nearly 75% unique small-molecule drugs contain a nitrogen heterocycle. Recently, Vitaku and colleagues published a comprehensive compilation of the structural diversity, substitution patterns and frequency of nitrogen heterocycles among U.S. FDA approved pharmaceuticals [14]. The N-heterocyclic skeletons feature significantly various classes of therapeutic applications and are used as the building blocks of a number of new drug candidates, due to the ability of the nitrogen atom to easily form hydrogen bonding with biological targets [15][16][17]. For example, pyrimidine derivatives have various therapeutic applications in medicinal chemistry and pyrimidine skeleton of thymine, cytosine, and uracil are essential building blocks of nucleic acids, DNA and RNA [18]. A vast number of nitrogen-containing heterocyclic compounds are known to exhibit a wide range of pharmacological activities including anticancer, anti-HIV, antimalaria, anti-tubercular, anti-microbial and diabetic activities [19][20][21][22][23][24][25]. A measure of their importance in the design of nitrogen heterocycles can be seen by the over 97,400 publications on nitrogen heterocycles that have appeared between 2009 and early 2020, as illustrated in Figure 1, of which 12,615 publications have been reported in the year 2019 [26]. Particularly, between the years 282 publications have been reported with medicinal chemistry related N-heterocycles. The utility of N-heterocyclic compounds is a hot spot in medicinal chemistry and chemical science, as can be seen by the list of nitrogen-containing clinical drugs ( Table 1). The exceptional role of nitrogen for variable interactions with biological targets, broadens the scope for drug development.
Molecules 2020, 25, x FOR PEER REVIEW 2 of 44 chemistry and allows them to bind with a variety of enzymes and receptors in biological targets with high affinity due to their improved solubility. The structural features of their derivatives are beneficial sice they exhibit broad bioactivities. A glance at the FDA databases reveals the structural significance of nitrogen-based heterocycles in drug design and engineering of pharmaceuticals. Nearly 75% unique small-molecule drugs contain a nitrogen heterocycle. Recently, Vitaku and colleagues published a comprehensive compilation of the structural diversity, substitution patterns and frequency of nitrogen heterocycles among U.S. FDA approved pharmaceuticals [14]. The N-heterocyclic skeletons feature significantly various classes of therapeutic applications and are used as the building blocks of a number of new drug candidates, due to the ability of the nitrogen atom to easily form hydrogen bonding with biological targets [15][16][17]. For example, pyrimidine derivatives have various therapeutic applications in medicinal chemistry and pyrimidine skeleton of thymine, cytosine, and uracil are essential building blocks of nucleic acids, DNA and RNA [18]. A vast number of nitrogen-containing heterocyclic compounds are known to exhibit a wide range of pharmacological activities including anticancer, anti-HIV, antimalaria, anti-tubercular, anti-microbial and diabetic activities [19][20][21][22][23][24][25]. A measure of their importance in the design of nitrogen heterocycles can be seen by the over 97,400 publications on nitrogen heterocycles that have appeared between 2009 and early 2020, as illustrated in Figure 1, of which 12,615 publications have been reported in the year 2019 [26]. Particularly, between the years 282 publications have been reported with medicinal chemistry related N-heterocycles. The utility of N-heterocyclic compounds is a hot spot in medicinal chemistry and chemical science, as can be seen by the list of nitrogen-containing clinical drugs ( Table 1). The exceptional role of nitrogen for variable interactions with biological targets, broadens the scope for drug development. This review abstracts information available over the past year (2019-2020) to provide an insight into the current status of nitrogen-containing heterocyclic molecules and their biological profile. The review evaluates the recent progress on different N-bearing heterocyclic structures (four-, five-and six-membered rings) introduced (i.e., β-lactams, pyrazoles, imidazoles, 1,2,4-triazoles, pyrimidines, quinolines, and quinazolines) and discusses their potential medicinal importance. An appraisal is provided for the rational design of more active molecules. We believe that this article will serve as an informative reference for the medicinally important nitrogen-containing heterocycles. This review abstracts information available over the past year (2019-2020) to provide an insight into the current status of nitrogen-containing heterocyclic molecules and their biological profile. The review evaluates the recent progress on different N-bearing heterocyclic structures (four-, five-and six-membered rings) introduced (i.e., β-lactams, pyrazoles, imidazoles, 1,2,4-triazoles, pyrimidines, quinolines, and quinazolines) and discusses their potential medicinal importance. An appraisal is provided for the rational design of more active molecules. We believe that this article will serve as an informative reference for the medicinally important nitrogen-containing heterocycles.

Four-Membered Ring Heterocycles
The nitrogen-containing four-membered ring heterocycles have proved their biological importance in medicinal chemistry and have further increased their biological significance.

β-Lactams
Among the heterocyclic compounds, the four-membered cyclic amide ring system of β-lactams has evolved as the scaffold of choice in the design of many antibiotics and it is also a valuable building block in organic synthesis. It is recognized as a vital component for the bioactivity profile of antibiotics [41][42][43][44][45]. A range of conjugates with diverse pharmacological applications can be synthesized by incorporating the β-lactam structure as a key scaffold or utilizing it as an important building block for the synthesis of a variety of bioactive heterocycles [46,47]. Besides antibiotics, βlactams have other clinical applications, for example, clavulanic acids are β-lactamase inhibitors and ezetimibe is a cholesterol absorption inhibitor ( Figure 2) [48,49]. These diverse applications have drawn attention to the further development of the β-lactam ring and several protocols have been devised to synthesize four-membered ring β-lactams. Currently, research in this area has been stimulated due to the development of bacterial resistance to known β-lactam antibiotics. Chromene tagged β-lactam molecular hybrids (1) were prepared and evaluated them for their anti-inflammatory and anticancer activities [50]. The authors used the RAW 264.7 murine macrophage assay to examine the anti-inflammatory behavior of the β-lactam hybrids, and evaluated the capacity of the compounds to inhibit the pro-inflammatory cascade, leading to NO production in mouse macrophages. Among the established molecules, compound 1a with a para-methylphenyl Folinic acid Anti-colorectal cancer [40] 22 Molecules 2020, 25, x FOR PEER REVIEW 7 of 44 Table 1. Cont.

Four-Membered Ring Heterocycles
The nitrogen-containing four-membered ring heterocycles have proved their biological importance in medicinal chemistry and have further increased their biological significance.

β-Lactams
Among the heterocyclic compounds, the four-membered cyclic amide ring system of β-lactams has evolved as the scaffold of choice in the design of many antibiotics and it is also a valuable building block in organic synthesis. It is recognized as a vital component for the bioactivity profile of antibiotics [41][42][43][44][45]. A range of conjugates with diverse pharmacological applications can be synthesized by incorporating the β-lactam structure as a key scaffold or utilizing it as an important building block for the synthesis of a variety of bioactive heterocycles [46,47]. Besides antibiotics, βlactams have other clinical applications, for example, clavulanic acids are β-lactamase inhibitors and ezetimibe is a cholesterol absorption inhibitor ( Figure 2) [48,49]. These diverse applications have drawn attention to the further development of the β-lactam ring and several protocols have been devised to synthesize four-membered ring β-lactams. Currently, research in this area has been stimulated due to the development of bacterial resistance to known β-lactam antibiotics. Chromene tagged β-lactam molecular hybrids (1) were prepared and evaluated them for their anti-inflammatory and anticancer activities [50]. The authors used the RAW 264.7 murine macrophage assay to examine the anti-inflammatory behavior of the β-lactam hybrids, and evaluated the capacity of the compounds to inhibit the pro-inflammatory cascade, leading to NO production in mouse macrophages. Among the established molecules, compound 1a with a para-methylphenyl moiety at N1 and para-chlorophenyl at the C4 position of the β-lactam ring showed the most

Four-Membered Ring Heterocycles
The nitrogen-containing four-membered ring heterocycles have proved their biological importance in medicinal chemistry and have further increased their biological significance.

β-Lactams
Among the heterocyclic compounds, the four-membered cyclic amide ring system of β-lactams has evolved as the scaffold of choice in the design of many antibiotics and it is also a valuable building block in organic synthesis. It is recognized as a vital component for the bioactivity profile of antibiotics [41][42][43][44][45]. A range of conjugates with diverse pharmacological applications can be synthesized by incorporating the β-lactam structure as a key scaffold or utilizing it as an important building block for the synthesis of a variety of bioactive heterocycles [46,47]. Besides antibiotics, β-lactams have other clinical applications, for example, clavulanic acids are β-lactamase inhibitors and ezetimibe is a cholesterol absorption inhibitor ( Figure 2) [48,49]. These diverse applications have drawn attention to the further development of the β-lactam ring and several protocols have been devised to synthesize four-membered ring β-lactams. Currently, research in this area has been stimulated due to the development of bacterial resistance to known β-lactam antibiotics.

Four-Membered Ring Heterocycles
The nitrogen-containing four-membered ring heterocycles have proved their biological importance in medicinal chemistry and have further increased their biological significance.

β-Lactams
Among the heterocyclic compounds, the four-membered cyclic amide ring system of β-lactams has evolved as the scaffold of choice in the design of many antibiotics and it is also a valuable building block in organic synthesis. It is recognized as a vital component for the bioactivity profile of antibiotics [41][42][43][44][45]. A range of conjugates with diverse pharmacological applications can be synthesized by incorporating the β-lactam structure as a key scaffold or utilizing it as an important building block for the synthesis of a variety of bioactive heterocycles [46,47]. Besides antibiotics, βlactams have other clinical applications, for example, clavulanic acids are β-lactamase inhibitors and ezetimibe is a cholesterol absorption inhibitor ( Figure 2) [48,49]. These diverse applications have drawn attention to the further development of the β-lactam ring and several protocols have been devised to synthesize four-membered ring β-lactams. Currently, research in this area has been stimulated due to the development of bacterial resistance to known β-lactam antibiotics. Chromene tagged β-lactam molecular hybrids (1) were prepared and evaluated them for their anti-inflammatory and anticancer activities [50]. The authors used the RAW 264.7 murine macrophage assay to examine the anti-inflammatory behavior of the β-lactam hybrids, and evaluated the capacity of the compounds to inhibit the pro-inflammatory cascade, leading to NO production in Chromene tagged β-lactam molecular hybrids (1) were prepared and evaluated them for their anti-inflammatory and anticancer activities [50]. The authors used the RAW 264.7 murine macrophage assay to examine the anti-inflammatory behavior of the β-lactam hybrids, and evaluated the capacity of the compounds to inhibit the pro-inflammatory cascade, leading to NO production in mouse macrophages. Among the established molecules, compound 1a with a para-methylphenyl moiety at N1 and para-chlorophenyl at the C4 position of the β-lactam ring showed the most significant activity with a 19.8 anti-inflammatory ratio (IC 50-NO release = 6.24 µM and IC 50-cell viability = 123.47 µM) (Figure 3). On the other hand, 4-chlorophenyl at N1 and 3-nitrophenyl at the C4 position of the β-lactam ring hybrid (1b) displayed the promising anticancer activity with an IC 50 value of 7.29 µM (compared to the standard methotrexate (IC 50 = 2.49 µM)) against the SW1116 colon cancer cell line. Structure-activity relationship (SAR) studies revealed that p-methoxy, p-tolyl and a p-chlorophenyl ring on the N1 of the β-lactam ring provided enhanced anticancer activity against the colon cancer (SW1116) cell line, whereas, a lower activity was observed against the HepG2 cell line for all the tested compounds. The replacement of the p-chlorophenyl group at the C-4 position of the lactam ring with a p-nitrophenyl group substantially decreased the in vitro anti-inflammatory activity. This loss of activity may be attributed to the weak π-π interaction between the lactam ring and enzyme active sites as discerned by docking studies. The active compound exhibited more lipophilic activity than other derivatives. Hence, the compound experiences stronger interactions with hydrophobic amino acids, accounting for its better anti-inflammatory activity.
Molecules 2020, 25, x FOR PEER REVIEW 8 of 44 β-lactam ring hybrid (1b) displayed the promising anticancer activity with an IC50 value of 7.29 M (compared to the standard methotrexate (IC50 = 2.49 M)) against the SW1116 colon cancer cell line. Structure-activity relationship (SAR) studies revealed that p-methoxy, p-tolyl and a p-chlorophenyl ring on the N1 of the β-lactam ring provided enhanced anticancer activity against the colon cancer (SW1116) cell line, whereas, a lower activity was observed against the HepG2 cell line for all the tested compounds. The replacement of the p-chlorophenyl group at the C-4 position of the lactam ring with a p-nitrophenyl group substantially decreased the in vitro anti-inflammatory activity. This loss of activity may be attributed to the weak π-π interaction between the lactam ring and enzyme active sites as discerned by docking studies. The active compound exhibited more lipophilic activity than other derivatives. Hence, the compound experiences stronger interactions with hydrophobic amino acids, accounting for its better anti-inflammatory activity. A series of β-lactam analogs 2 were synthesized and screened the analogs for antiproliferative activity against human colon (HT-29) and breast (MCF-7) cancer cell lines [51]. A hydroxy attached at the 3-position of the β-lactam and a fluorophenyl conjugate (compound 2a) showed excellent antiproliferative activity with IC50 values of 0.022 M against MCF-7 and 0.003 M against HT-29 cell lines ( Figure 4). Reduced activity was observed when the chloro, bromo, and iodo substituents were replaced with a fluorine atom with the following order Br > Cl > I against the colon HT-29 cell line. The introduction of fluorine may improve the pharmacological and physicochemical properties, such as metabolic stability, lipophilicity, and ligand binding of a compound. The active compound, 2a, induced mitotic arrest (G2/M phase) at a micromolar concentration in both colon and breast cancer cell lines and strongly inhibited the colchicine site of tubulin. The 3,4,5-trimethoxyphenyl group can make favorable van der Waals contacts within the lower subpocket delineated by Valb318 and Cysb241. The overlap of the fluorine atom onto the DAMA-colchicine carbonyl oxygen atom facilitates the HBA interaction with Lysb352, and the hydroxyl group on the β-lactam ring can form an HDB interaction with Lys254. From the molecular modeling study, it can be concluded that the A series of β-lactam analogs 2 were synthesized and screened the analogs for antiproliferative activity against human colon (HT-29) and breast (MCF-7) cancer cell lines [51]. A hydroxy attached at the 3-position of the β-lactam and a fluorophenyl conjugate (compound 2a) showed excellent antiproliferative activity with IC 50 values of 0.022 µM against MCF-7 and 0.003 µM against HT-29 cell lines ( Figure 4). Reduced activity was observed when the chloro, bromo, and iodo substituents were replaced with a fluorine atom with the following order Br > Cl > I against the colon HT-29 cell line. The introduction of fluorine may improve the pharmacological and physicochemical properties, such as metabolic stability, lipophilicity, and ligand binding of a compound. The active compound, 2a, induced mitotic arrest (G2/M phase) at a micromolar concentration in both colon and breast cancer cell lines and strongly inhibited the colchicine site of tubulin. The 3,4,5-trimethoxyphenyl group can make favorable Molecules 2020, 25, 1909 7 of 42 van der Waals contacts within the lower subpocket delineated by Valb318 and Cysb241. The overlap of the fluorine atom onto the DAMA-colchicine carbonyl oxygen atom facilitates the HBA interaction with Lysb352, and the hydroxyl group on the β-lactam ring can form an HDB interaction with Lys254. From the molecular modeling study, it can be concluded that the active compound interacts with tubulin at the same site as colchicine with similar binding modes. The β-lactam derivatives are promising candidates in the progress of tubulin-targeting agents for the treatment of colon and breast cancers.  A series of β-lactam-anthraquinone hybrids 3 were manufactured as potential antibacterial and antifungal agents [52]. Among them, the thio-methyl substituent at C-3 and the 3,4,5-trimethoxy phenyl group at the C-4 position of the β-lactam ring scaffold (compound 3a) exhibited the most potent antibacterial activity (MIC = 0.25 g/mL) against the Staphylococcus aureus bacterial strain, compared with the standard ciprofloxacin (MIC = 0.5 g/mL) ( Figure 5). The same compound displayed equal antifungal activity (MIC = 4 g/mL) as the reference ciclopirox olamine (MIC = 4 g/mL) against the Candida albicans strain. It was found that anthraquinone-β-lactam derivatives, which containing a 2-naphtho group at the C-3 position, and 4-chloro and 4-methoxyphenyl groups at C-4 of the β-lactam ring showed lower activity, which can be attributed to steric hindrance that weakens the intermolecular interactions. Furthermore, the active hybrid was evaluated by molecular docking studies and the carbonyl groups of the anthraquinone moiety and the β-lactam ring had three hydrogen bonding interactions with Lys273, Asp295 and Val277 residuces, while the MeS group interacted with the active site of Tyr272 and the 3,4,5-trimethoxyphenyl group exerted hydrophobic interactions with His293, Lys289, Gln292 and Lys319 residues of a penicillin-binding protein (PBP). β-Lactams and their derivatives 4 were synthesized and evaluated for antimicrobial activity against Mycobacterium tuberculosis (M.tb) and Moraxella catarrhalis (M.cat) [53]. Among them, the meta- A series of β-lactam-anthraquinone hybrids 3 were manufactured as potential antibacterial and antifungal agents [52]. Among them, the thio-methyl substituent at C-3 and the 3,4,5-trimethoxy phenyl group at the C-4 position of the β-lactam ring scaffold (compound 3a) exhibited the most potent antibacterial activity (MIC = 0.25 µg/mL) against the Staphylococcus aureus bacterial strain, compared with the standard ciprofloxacin (MIC = 0.5 µg/mL) ( Figure 5). The same compound displayed equal antifungal activity (MIC = 4 µg/mL) as the reference ciclopirox olamine (MIC = 4 µg/mL) against the Candida albicans strain. It was found that anthraquinone-β-lactam derivatives, which containing a 2-naphtho group at the C-3 position, and 4-chloro and 4-methoxyphenyl groups at C-4 of the β-lactam ring showed lower activity, which can be attributed to steric hindrance that weakens the intermolecular interactions. Furthermore, the active hybrid was evaluated by molecular docking studies and the carbonyl groups of the anthraquinone moiety and the β-lactam ring had three hydrogen bonding interactions with Lys273, Asp295 and Val277 residuces, while the MeS group interacted with the active site of Tyr272 and the 3,4,5-trimethoxyphenyl group exerted hydrophobic interactions with His293, Lys289, Gln292 and Lys319 residues of a penicillin-binding protein (PBP).  A series of β-lactam-anthraquinone hybrids 3 were manufactured as potential antibacterial and antifungal agents [52]. Among them, the thio-methyl substituent at C-3 and the 3,4,5-trimethoxy phenyl group at the C-4 position of the β-lactam ring scaffold (compound 3a) exhibited the most potent antibacterial activity (MIC = 0.25 g/mL) against the Staphylococcus aureus bacterial strain, compared with the standard ciprofloxacin (MIC = 0.5 g/mL) ( Figure 5). The same compound displayed equal antifungal activity (MIC = 4 g/mL) as the reference ciclopirox olamine (MIC = 4 g/mL) against the Candida albicans strain. It was found that anthraquinone-β-lactam derivatives, which containing a 2-naphtho group at the C-3 position, and 4-chloro and 4-methoxyphenyl groups at C-4 of the β-lactam ring showed lower activity, which can be attributed to steric hindrance that weakens the intermolecular interactions. Furthermore, the active hybrid was evaluated by molecular docking studies and the carbonyl groups of the anthraquinone moiety and the β-lactam ring had three hydrogen bonding interactions with Lys273, Asp295 and Val277 residuces, while the MeS group interacted with the active site of Tyr272 and the 3,4,5-trimethoxyphenyl group exerted hydrophobic interactions with His293, Lys289, Gln292 and Lys319 residues of a penicillin-binding protein (PBP). β-Lactams and their derivatives 4 were synthesized and evaluated for antimicrobial activity against Mycobacterium tuberculosis (M.tb) and Moraxella catarrhalis (M.cat) [53]. Among them, the meta-CF3 of the phenylthiol ring and the achiral carbamyl group at the lactam nitrogen showed potent activity against M.tb (MIC = 25 g/mL) and M.cat (MIC = 1.5 g/mL) ( Figure 6). In contrast, a β-Lactams and their derivatives 4 were synthesized and evaluated for antimicrobial activity against Mycobacterium tuberculosis (M.tb) and Moraxella catarrhalis (M.cat) [53]. Among them, the meta-CF 3 of the phenylthiol ring and the achiral carbamyl group at the lactam nitrogen showed potent activity against M.tb (MIC = 25 µg/mL) and M.cat (MIC = 1.5 µg/mL) ( Figure 6). In contrast, a decreasing activity was found with para-CF 3 , fluorine (para-, metaand ortho-), and difluoro groups substituted on the phenylthiol ring analogs. Moreover, the addition of the achiral carbamyl group enhanced anti-Mtb activity relative to the unsubstituted derivative. The lack of a substantial difference in the activity against M.cat and M.tb may be due to nonspecific binding of the respective compounds to hydrophobic medium components. The active compound showed good activity against non-replicating and multi-drug resistant Mtb.
Molecules 2020, 25, x FOR PEER REVIEW 10 of 44 enhanced anti-Mtb activity relative to the unsubstituted derivative. The lack of a substantial difference in the activity against M.cat and M.tb may be due to nonspecific binding of the respective compounds to hydrophobic medium components. The active compound showed good activity against non-replicating and multi-drug resistant Mtb.

1,2,3-Triazoles
The 1,2,3-triazole moiety is the main pharmacophore system among the nitrogen-based molecules and is a privileged building block in the discovery of various new biological targets. These five-membered heterocyclic motifs with three nitrogen heteroatoms can be easily prepared via 'click' chemistry, i.e., by copper-catalyzed azide-alkyne cycloaddition [Cu-AAC] reactions. Generally, the 'linker' property of 1,2,3-triazoles is stable to hydrolysis under acidic or basic conditions, and metabolic degradation. These compounds can interact with different biological targets through hydrogen bonding, noncovalent and van der Waals interactions as well as dipole-dipole bonding interactions. Furthermore, triazoles are weakly acidic and weakly basic and are more sensitive to reducing agents. Moreover, the 1,2,3-triazole-based compound, carboxyamidotriazole, has been successfully clinically evaluated for cancer treatment (Figure 7) [54][55][56]. Furthermore, the strong dipole properties of the triazole unit increased its importance in the field of medicinal chemistry, as it binds to the biological target with high affinity.

1,2,3-Triazoles
The 1,2,3-triazole moiety is the main pharmacophore system among the nitrogen-based molecules and is a privileged building block in the discovery of various new biological targets. These five-membered heterocyclic motifs with three nitrogen heteroatoms can be easily prepared via 'click' chemistry, i.e., by copper-catalyzed azide-alkyne cycloaddition [Cu-AAC] reactions. Generally, the 'linker' property of 1,2,3-triazoles is stable to hydrolysis under acidic or basic conditions, and metabolic degradation. These compounds can interact with different biological targets through hydrogen bonding, noncovalent and van der Waals interactions as well as dipole-dipole bonding interactions. Furthermore, triazoles are weakly acidic and weakly basic and are more sensitive to reducing agents. Moreover, the 1,2,3-triazole-based compound, carboxyamidotriazole, has been successfully clinically evaluated for cancer treatment (Figure 7) [54][55][56]. Furthermore, the strong dipole properties of the triazole unit increased its importance in the field of medicinal chemistry, as it binds to the biological target with high affinity. metabolic degradation. These compounds can interact with different biological targets through hydrogen bonding, noncovalent and van der Waals interactions as well as dipole-dipole bonding interactions. Furthermore, triazoles are weakly acidic and weakly basic and are more sensitive to reducing agents. Moreover, the 1,2,3-triazole-based compound, carboxyamidotriazole, has been successfully clinically evaluated for cancer treatment (Figure 7) [54][55][56]. Furthermore, the strong dipole properties of the triazole unit increased its importance in the field of medicinal chemistry, as it binds to the biological target with high affinity. Phenothiazine conjugates 5 and 6 with a 1,2,3-triazole linker were developed and the conjugates screened for anti-tubercular activity against Mycobacterium tuberculosis H37Rv strain [57]. All the Phenothiazine conjugates 5 and 6 with a 1,2,3-triazole linker were developed and the conjugates screened for anti-tubercular activity against Mycobacterium tuberculosis H37Rv strain [57]. All the compounds exhibited relatively good in vitro anti-tubercular activity as demonstrated by the compound 5a bearing a 4-nitro group that showed significant anti-tubercular activity with a MIC value of 2.44 µM against M.tb H37Rv (Figure 8). Compound 5a also displayed non-toxic characteristics against VERO cell lines. The isonicotinohydrazide/nicotinohydrazide derivatives 6 exhibited moderate activity with MIC values ranging from 2.61-2.94 µM against M.tb H37Rv. A SAR study revealed that the substituted phenyl rings, instead of the isonicotinohydrazide/ nicotinohydrazide resulted in highly potent activity, which was dependent on the substituents' electronic effect on the phenyl ring. The active compound was comfortably docked into the Inh A and Cyp121 enzymes. Compound 5a was exhibited distinct hydrogen bonding formation and pi-pi interactions with the hydrophobic residue Tyr158 of the Inh A enzyme. The most potent compound displayed higher permeability and aqueous solubility values than the respective analogs. In addition to, the pharmacokinetic parameters suggested that the compound exhibits good oral bioavailability. The potent compound signified a novel hybrid for the development of potential anti-tubercular agents. A SAR study revealed that the substituted phenyl rings, instead of the isonicotinohydrazide/ nicotinohydrazide resulted in highly potent activity, which was dependent on the substituents' electronic effect on the phenyl ring. The active compound was comfortably docked into the Inh A and Cyp121 enzymes. Compound 5a was exhibited distinct hydrogen bonding formation and pi-pi interactions with the hydrophobic residue Tyr158 of the Inh A enzyme. The most potent compound displayed higher permeability and aqueous solubility values than the respective analogs. In addition to, the pharmacokinetic parameters suggested that the compound exhibits good oral bioavailability. The potent compound signified a novel hybrid for the development of potential anti-tubercular agents. Phenylalanine scaffolds 7 with 1,2,3-triazole linkers were synthesized and tested for their antiviral activity in TZM-bl cells infected with the HIV-1 NL4-3 virus [58]. Most of the compounds displayed excellent activity with EC50 values ranging from 3.13-16.48 μM against HIV-1 in TZM-bl cells. Among them, the 2-fluoro benzamide compound 7a exhibited high anti-HIV activity (EC50 = 3.13 μM) and showed very low toxicity (CC50  16.48 μM, Figure 9).  Phenylalanine scaffolds 7 with 1,2,3-triazole linkers were synthesized and tested for their antiviral activity in TZM-bl cells infected with the HIV-1 NL4-3 virus [58]. Most of the compounds displayed excellent activity with EC 50 values ranging from 3.13-16.48 µM against HIV-1 in TZM-bl cells. Among them, the 2-fluoro benzamide compound 7a exhibited high anti-HIV activity (EC 50 = 3.13 µM) and showed very low toxicity (CC 50 ≥ 16.48 µM, Figure 9). SAR studies revealed that the ortho-substituted aniline derivatives displayed higher activity than the paraand meta-substituted analogs. Particularly, fluorine substituted on the phenyl ring conjugates showed promissing antiviral activity. Furthermore, the binding mode of compound 7a within the active site of the HIV-1 capsid (CA) monomer protein was confirmed. The triazole linked phenyl ring was formed a hydrophobic interaction with Met66 and an aliphatic hydrogen bonding could be formed between the methoxy group and Lys70 backbone in the first cluster and the Asn74 backbone in the second cluster. These results show that the large and smaller flexible groups of the 1,2,3-triazole moiety play a significant part in increasing the anti-HIV activity. Phenylalanine scaffolds 7 with 1,2,3-triazole linkers were synthesized and tested for their antiviral activity in TZM-bl cells infected with the HIV-1 NL4-3 virus [58]. Most of the compounds displayed excellent activity with EC50 values ranging from 3.13-16.48 μM against HIV-1 in TZM-bl cells. Among them, the 2-fluoro benzamide compound 7a exhibited high anti-HIV activity (EC50 = 3.13 μM) and showed very low toxicity (CC50  16.48 μM, Figure 9).  1,2,3-Triazole-linked trimethoxyphenyl scaffolds 8 were prepared and screened for antiproliferative activity against prostate (PC3), liver (HepG2) and gastric (MGC803) cancer cell lines [59]. Among the synthesized hybrids, the coumarin moiety allied to a 1,2,3-triazole (compound 8a) exhibited promising antiproliferative activity (IC 50 = 0.13 µM) against the MGC803 cell line ( Figure 10), compared with the standard drug colchicine (IC 50 = 0.27 µM). Replacement of the coumarin moiety with a phenothiazine ring and were observed decreased activity. Compound 8a could inhibit MGC803 cell growth and colony formation. It caused cell cycle arrest at the G2/M phase and tubulin polymerization was sturdily inhibited by correlating with the colchicine site. Furthermore, when the hybrid 8a was validated by molecular docking studies it was found that the 3,4,5-trimethoxyphenyl ring, 4-methoxyphenyl ring, and the amide group formed three hydrogen bonding with the Asn329, Tyr224 and Lys352 residues at the active site of tubulin. These results revealed that coumarin with 1,2,3-triazole hybrids are potent colchicine site tubulin polymerization inhibitors. SAR studies revealed that the ortho-substituted aniline derivatives displayed higher activity than the para-and meta-substituted analogs. Particularly, fluorine substituted on the phenyl ring conjugates showed promissing antiviral activity. Furthermore, the binding mode of compound 7a within the active site of the HIV-1 capsid (CA) monomer protein was confirmed. The triazole linked phenyl ring was formed a hydrophobic interaction with Met66 and an aliphatic hydrogen bonding could be formed between the methoxy group and Lys70 backbone in the first cluster and the Asn74 backbone in the second cluster. These results show that the large and smaller flexible groups of the 1,2,3-triazole moiety play a significant part in increasing the anti-HIV activity.
1,2,3-Triazole-linked trimethoxyphenyl scaffolds 8 were prepared and screened for antiproliferative activity against prostate (PC3), liver (HepG2) and gastric (MGC803) cancer cell lines [59]. Among the synthesized hybrids, the coumarin moiety allied to a 1,2,3-triazole (compound 8a) exhibited promising antiproliferative activity (IC50 = 0.13 M) against the MGC803 cell line ( Figure  10), compared with the standard drug colchicine (IC50 = 0.27 M). Replacement of the coumarin moiety with a phenothiazine ring and were observed decreased activity. Compound 8a could inhibit MGC803 cell growth and colony formation. It caused cell cycle arrest at the G2/M phase and tubulin polymerization was sturdily inhibited by correlating with the colchicine site. Furthermore, when the hybrid 8a was validated by molecular docking studies it was found that the 3,4,5-trimethoxyphenyl ring, 4-methoxyphenyl ring, and the amide group formed three hydrogen bonding with the Asn329, Tyr224 and Lys352 residues at the active site of tubulin. These results revealed that coumarin with 1,2,3-triazole hybrids are potent colchicine site tubulin polymerization inhibitors. A library of chalcone conjugates 9 with a 1,2,3-triazole linker were synthesized and the conjugates screened for in vitro antiproliferative activity [60]. All the hybrids exhibited promising proliferative activity against a panel of 60 human cancer cell lines (NCI). The 3,4-dimethoxy and 4chlorophenyl ring derivative 9a displayed greater cytotoxic activity with IC50 values of 0.24 and 0.26 M against the breast MCF7 and colon HCT116 cancer cell lines, respectively ( Figure 11). The strong electron-withdrawing group (NO2) showed less activity than the chloro substituents. This observation may suggested that the electronic and steric properties of the substituents play an important role in the binding affinity of chalcones to their cellular target. The potent hybrid induced apoptosis via aggregation of MM RPMI-8226 cells in a dose-dependent manner and cell cycle arrest at the G2/M phase. Compound 9a could be a potential lead for further advancement of anticancer studies and may offer new insights in treating multiple myeloma RPMI-8226. A library of chalcone conjugates 9 with a 1,2,3-triazole linker were synthesized and the conjugates screened for in vitro antiproliferative activity [60]. All the hybrids exhibited promising proliferative activity against a panel of 60 human cancer cell lines (NCI). The 3,4-dimethoxy and 4-chlorophenyl ring derivative 9a displayed greater cytotoxic activity with IC 50 values of 0.24 and 0.26 µM against the breast MCF7 and colon HCT116 cancer cell lines, respectively ( Figure 11). The strong electron-withdrawing group (NO 2 ) showed less activity than the chloro substituents. This observation may suggested that the electronic and steric properties of the substituents play an important role in the binding affinity of chalcones to their cellular target. The potent hybrid induced apoptosis via aggregation of MM RPMI-8226 cells in a dose-dependent manner and cell cycle arrest at the G2/M phase. Compound 9a could be a potential lead for further advancement of anticancer studies and may offer new insights in treating multiple myeloma RPMI-8226. Biscoumarin compounds 10 with a 1,2,3-triazole linker were synthesized and screened the compounds for their in vitro α-glucosidase inhibitory activity as potential anti-diabetic agents [61]. Among them the screened hybrids, the compounds bearing with a 2-chloro (10a; IC50 = 13.0 M) and 2-methyl (10b; IC50 = 16.4 M) exhibited better α-glucosidase inhibitory activity than the standard drug acarbose (IC50 = 750.0 M) ( Figure 12). Changing the position of the chlorine atom in the phenyl ring from C-2 to C-4, led to a reduction in inhibitory activity. SAR studies revealed that the orthosubstituted phenyl hybrids showed highest activity than the para-substituted derivatives. The SAR evaluation also indicated electron-donating groups provided greater activity than electronwithdrawing groups. Furthermore, the compound 10a was evaluated by molecular docking studies with active sites of the α-glucosidase enzyme. The results showed that the active compound 10a formed a hydrogen bonding with residue Thr307, one of the coumarin rings of this compound formed a π-π interaction with His279 and the other coumarin ring interacted with Val305 via hydrophobic interaction, while the 2-chlorophenyl group established a π-anion interaction with Asp408 through phenyl ring and two hydrophobic interactions with Tyr313 and Phe311 through the 2-chloro substituent. These compounds are potential candidates for the development of novel anti-diabetic agents.  Biscoumarin compounds 10 with a 1,2,3-triazole linker were synthesized and screened the compounds for their in vitro α-glucosidase inhibitory activity as potential anti-diabetic agents [61]. Among them the screened hybrids, the compounds bearing with a 2-chloro (10a; IC 50 = 13.0 µM) and 2-methyl (10b; IC 50 = 16.4 µM) exhibited better α-glucosidase inhibitory activity than the standard drug acarbose (IC 50 = 750.0 µM) ( Figure 12). Changing the position of the chlorine atom in the phenyl ring from C-2 to C-4, led to a reduction in inhibitory activity. SAR studies revealed that the ortho-substituted phenyl hybrids showed highest activity than the para-substituted derivatives. The SAR evaluation also indicated electron-donating groups provided greater activity than electron-withdrawing groups. Furthermore, the compound 10a was evaluated by molecular docking studies with active sites of the α-glucosidase enzyme. The results showed that the active compound 10a formed a hydrogen bonding with residue Thr307, one of the coumarin rings of this compound formed a π-π interaction with His279 and the other coumarin ring interacted with Val305 via hydrophobic interaction, while the 2-chlorophenyl group established a π-anion interaction with Asp408 through phenyl ring and two hydrophobic interactions with Tyr313 and Phe311 through the 2-chloro substituent. These compounds are potential candidates for the development of novel anti-diabetic agents. Biscoumarin compounds 10 with a 1,2,3-triazole linker were synthesized and screened the compounds for their in vitro α-glucosidase inhibitory activity as potential anti-diabetic agents [61]. Among them the screened hybrids, the compounds bearing with a 2-chloro (10a; IC50 = 13.0 M) and 2-methyl (10b; IC50 = 16.4 M) exhibited better α-glucosidase inhibitory activity than the standard drug acarbose (IC50 = 750.0 M) ( Figure 12). Changing the position of the chlorine atom in the phenyl ring from C-2 to C-4, led to a reduction in inhibitory activity. SAR studies revealed that the orthosubstituted phenyl hybrids showed highest activity than the para-substituted derivatives. The SAR evaluation also indicated electron-donating groups provided greater activity than electronwithdrawing groups. Furthermore, the compound 10a was evaluated by molecular docking studies with active sites of the α-glucosidase enzyme. The results showed that the active compound 10a formed a hydrogen bonding with residue Thr307, one of the coumarin rings of this compound formed a π-π interaction with His279 and the other coumarin ring interacted with Val305 via hydrophobic interaction, while the 2-chlorophenyl group established a π-anion interaction with Asp408 through phenyl ring and two hydrophobic interactions with Tyr313 and Phe311 through the 2-chloro substituent. These compounds are potential candidates for the development of novel anti-diabetic agents.  A series of quinazolinone hybrids 11 with a 1,2,3-triazole linker were reported by Saeedi and co-workers and the hybrids evaluated for their in vitro α-glucosidase inhibitory activity [62]. All the screened hybrids exhibited good inhibitory activity with IC 50 values ranging from 181.0-474.5 µM compared with the reference acarbose with an IC 50 value of 750.0 µM ( Figure 13). Compound 11a substituted with 4-bromobenzyl moiety exhibited highest α-glucosidase inhibitory activity (IC 50 = 181.0 µM). SAR studies revealed that weak inhibitory activity was found when fluorine and chlorine atoms substituted the benzyl moieties unlike with the bromo compound 11a. A slightly reduced activity was observed when the bromine atom was substituted on the ortho and meta-positions of the benzyl ring. Furthermore, by molecular docking studies in the active site of α-glucosidase, the quinazolinone moiety of hybrid 11a was shown to form a hydrogen bonding and a π-π interaction with His279, and the phenethyl group and sulfur atom interacted with Arg312 residues. Hydrophobic interactions between Pro309 and the 1,2,3-triazole ring were also observed. Moreover, the 4-bromobenzyl group showed two interactions with Val305 and Val316 through the 4-bromo substituent and a hydrophobic interaction with Pro309 through the phenyl ring. Quinazolinone linked 1,2,3-triazole hybrids are potential conjugates for the treatment of antidiabetic activity. A series of quinazolinone hybrids 11 with a 1,2,3-triazole linker were reported by Saeedi and coworkers and the hybrids evaluated for their in vitro α-glucosidase inhibitory activity [62]. All the screened hybrids exhibited good inhibitory activity with IC50 values ranging from 181.0-474.5 M compared with the reference acarbose with an IC50 value of 750.0 M ( Figure 13). Compound 11a substituted with 4-bromobenzyl moiety exhibited highest α-glucosidase inhibitory activity (IC50 = 181.0 M). SAR studies revealed that weak inhibitory activity was found when fluorine and chlorine atoms substituted the benzyl moieties unlike with the bromo compound 11a. A slightly reduced activity was observed when the bromine atom was substituted on the ortho and meta-positions of the benzyl ring. Furthermore, by molecular docking studies in the active site of α-glucosidase, the quinazolinone moiety of hybrid 11a was shown to form a hydrogen bonding and a π-π interaction with His279, and the phenethyl group and sulfur atom interacted with Arg312 residues. Hydrophobic interactions between Pro309 and the 1,2,3-triazole ring were also observed. Moreover, the 4-bromobenzyl group showed two interactions with Val305 and Val316 through the 4-bromo substituent and a hydrophobic interaction with Pro309 through the phenyl ring. Quinazolinone linked 1,2,3-triazole hybrids are potential conjugates for the treatment of antidiabetic activity. Saeedi's group [63] investigated imidazole hybrids 12 with a 1,2,3-triazole linker and evaluated them for in vitro α-glucosidase inhibitory activity as a potential anti-diabetic agents. Among them, the phenyl ring hybrids with 3,5-dimethyl (12a) and 2,3-dichloro (12b) substitution showed the most potent inhibitory activity with IC50 values of 90.4 M and 97.7 M, respectively ( Figure 14). Compounds 12a and 12b were 8-fold more effective than the reference acarbose (IC50 = 750.0 M). Compounds bearing electron-donating groups showed higher inhibitory activity than those with electron-withdrawing groups. It can be concluded that the substituents on the benzyl moiety connected to the 1,2,3-triazole ring played a remarkable role for in vitro α-glucosidase inhibitory activity. Furthermore, the binding mode of the active hybrid 12a fitted well into the active site of α- Saeedi's group [63] investigated imidazole hybrids 12 with a 1,2,3-triazole linker and evaluated them for in vitro α-glucosidase inhibitory activity as a potential anti-diabetic agents. Among them, the phenyl ring hybrids with 3,5-dimethyl (12a) and 2,3-dichloro (12b) substitution showed the most potent inhibitory activity with IC 50 values of 90.4 µM and 97.7 µM, respectively ( Figure 14). A series of quinazolinone hybrids 11 with a 1,2,3-triazole linker were reported by Saeedi and coworkers and the hybrids evaluated for their in vitro α-glucosidase inhibitory activity [62]. All the screened hybrids exhibited good inhibitory activity with IC50 values ranging from 181.0-474.5 M compared with the reference acarbose with an IC50 value of 750.0 M ( Figure 13). Compound 11a substituted with 4-bromobenzyl moiety exhibited highest α-glucosidase inhibitory activity (IC50 = 181.0 M). SAR studies revealed that weak inhibitory activity was found when fluorine and chlorine atoms substituted the benzyl moieties unlike with the bromo compound 11a. A slightly reduced activity was observed when the bromine atom was substituted on the ortho and meta-positions of the benzyl ring. Furthermore, by molecular docking studies in the active site of α-glucosidase, the quinazolinone moiety of hybrid 11a was shown to form a hydrogen bonding and a π-π interaction with His279, and the phenethyl group and sulfur atom interacted with Arg312 residues. Hydrophobic interactions between Pro309 and the 1,2,3-triazole ring were also observed. Moreover, the 4-bromobenzyl group showed two interactions with Val305 and Val316 through the 4-bromo substituent and a hydrophobic interaction with Pro309 through the phenyl ring. Quinazolinone linked 1,2,3-triazole hybrids are potential conjugates for the treatment of antidiabetic activity. Saeedi's group [63] investigated imidazole hybrids 12 with a 1,2,3-triazole linker and evaluated them for in vitro α-glucosidase inhibitory activity as a potential anti-diabetic agents. Among them, the phenyl ring hybrids with 3,5-dimethyl (12a) and 2,3-dichloro (12b) substitution showed the most potent inhibitory activity with IC50 values of 90.4 M and 97.7 M, respectively ( Figure 14). Compounds 12a and 12b were 8-fold more effective than the reference acarbose (IC50 = 750.0 M). Compounds bearing electron-donating groups showed higher inhibitory activity than those with electron-withdrawing groups. It can be concluded that the substituents on the benzyl moiety connected to the 1,2,3-triazole ring played a remarkable role for in vitro α-glucosidase inhibitory activity. Furthermore, the binding mode of the active hybrid 12a fitted well into the active site of α- Compounds 12a and 12b were 8-fold more effective than the reference acarbose (IC 50 = 750.0 µM). Compounds bearing electron-donating groups showed higher inhibitory activity than those with electron-withdrawing groups. It can be concluded that the substituents on the benzyl moiety connected to the 1,2,3-triazole ring played a remarkable role for in vitro α-glucosidase inhibitory activity. Furthermore, the binding mode of the active hybrid 12a fitted well into the active site of α-glucosidase with two hydrogen-bonding interactions between the NH of the imidazole moiety and Thr307, as well as the nitrogen of 1,2,3-triazole ring and Arg312. Furthermore, two hydrophobic interactions were notable between the 3,5-dimethylbenzyl moiety and the Phe300 and Arg439 residues.
Aminonaphthoquinone conjugates 13 bearing a 1,2,3-triazole linker were synthesized and the conjugates evaluated for their cytotoxicity activity against three human cancer cell lines, namely MCF-7, MOLT-4, and HT-29 [64]. The hybrid with a 4-trifluoromethyl group substituent on the benzyl moiety (compound 13a) displayed excellent activity with an IC 50 value of 10.4 µM against the breast cancer (MCF-7) cell line, as compared to cisplatin (IC 50 = 8.8 µM) as a reference drug ( Figure 15). The introduction of the 4-nitro group diminished the lipophilic character of the hybrid, making the compound completely inactive against all the three cancer cell lines. The active hybrid 13a caused cell cycle arrest at the G0/G1 phase in the breast cancer MCF-7 cells.
Molecules 2020, 25, x FOR PEER REVIEW 15 of 44 glucosidase with two hydrogen-bonding interactions between the NH of the imidazole moiety and Thr307, as well as the nitrogen of 1,2,3-triazole ring and Arg312. Furthermore, two hydrophobic interactions were notable between the 3,5-dimethylbenzyl moiety and the Phe300 and Arg439 residues. Aminonaphthoquinone conjugates 13 bearing a 1,2,3-triazole linker were synthesized and the conjugates evaluated for their cytotoxicity activity against three human cancer cell lines, namely MCF-7, MOLT-4, and HT-29 [64]. The hybrid with a 4-trifluoromethyl group substituent on the benzyl moiety (compound 13a) displayed excellent activity with an IC50 value of 10.4 M against the breast cancer (MCF-7) cell line, as compared to cisplatin (IC50 = 8.8 M) as a reference drug ( Figure  15). The introduction of the 4-nitro group diminished the lipophilic character of the hybrid, making the compound completely inactive against all the three cancer cell lines. The active hybrid 13a caused cell cycle arrest at the G0/G1 phase in the breast cancer MCF-7 cells.

Imidazoles and Benzoimidazoles
The unique structural features of the five-membered imidazole and benzoimidazole moieties distinguish them as important heterocycles and these structures are part of many natural products and synthetic compounds. The electron-rich nature of the imidazole-based derivatives is useful to freely bind with various receptors and enzymes in the biological profile, thus showing broad biological activities [65,66]. Several imidazole-based molecules (oxiconazole, dacarbazine, and clotrimazole) exhibiting antifungal and anticancer activities with high therapeutic potency are clinically used as drugs ( Figure 16). The scope of imidazole-and benzoimidazole-based molecules increasing rapidly in medicinal chemistry. Benzoimidazole-quinazolinone hybrids 14 were synthesized and tested the molecules for their cytotoxicity and Aurora-A kinase inhibitory activity by MTT assay [67]. All the tested hybrids showed good cytotoxicity activity, with IC50 values ranging from 0.38 to 18.13 M against three cancer cell lines namely breast cancer (MDA-MB-231), prostate cancer (PC3) and neuroblastoma (SH-SY5Y) ( Figure 17). Among them, the morpholinoethyl compound 14a displayed excellent activity with an IC50 value of 0.38 M (MDA-MB-231), 1.09 M (PC3) and 0.77 M (SH-SY5Y). Compound 14a also showed promising inhibitory activity (IC50 = 21.94 M) against Aurora-A kinase. In addition to,

Imidazoles and Benzoimidazoles
The unique structural features of the five-membered imidazole and benzoimidazole moieties distinguish them as important heterocycles and these structures are part of many natural products and synthetic compounds. The electron-rich nature of the imidazole-based derivatives is useful to freely bind with various receptors and enzymes in the biological profile, thus showing broad biological activities [65,66]. Several imidazole-based molecules (oxiconazole, dacarbazine, and clotrimazole) exhibiting antifungal and anticancer activities with high therapeutic potency are clinically used as drugs ( Figure 16). The scope of imidazole-and benzoimidazole-based molecules increasing rapidly in medicinal chemistry.
Molecules 2020, 25, x FOR PEER REVIEW 15 of 44 glucosidase with two hydrogen-bonding interactions between the NH of the imidazole moiety and Thr307, as well as the nitrogen of 1,2,3-triazole ring and Arg312. Furthermore, two hydrophobic interactions were notable between the 3,5-dimethylbenzyl moiety and the Phe300 and Arg439 residues. Aminonaphthoquinone conjugates 13 bearing a 1,2,3-triazole linker were synthesized and the conjugates evaluated for their cytotoxicity activity against three human cancer cell lines, namely MCF-7, MOLT-4, and HT-29 [64]. The hybrid with a 4-trifluoromethyl group substituent on the benzyl moiety (compound 13a) displayed excellent activity with an IC50 value of 10.4 M against the breast cancer (MCF-7) cell line, as compared to cisplatin (IC50 = 8.8 M) as a reference drug ( Figure  15). The introduction of the 4-nitro group diminished the lipophilic character of the hybrid, making the compound completely inactive against all the three cancer cell lines. The active hybrid 13a caused cell cycle arrest at the G0/G1 phase in the breast cancer MCF-7 cells.

Imidazoles and Benzoimidazoles
The unique structural features of the five-membered imidazole and benzoimidazole moieties distinguish them as important heterocycles and these structures are part of many natural products and synthetic compounds. The electron-rich nature of the imidazole-based derivatives is useful to freely bind with various receptors and enzymes in the biological profile, thus showing broad biological activities [65,66]. Several imidazole-based molecules (oxiconazole, dacarbazine, and clotrimazole) exhibiting antifungal and anticancer activities with high therapeutic potency are clinically used as drugs ( Figure 16). The scope of imidazole-and benzoimidazole-based molecules increasing rapidly in medicinal chemistry. Benzoimidazole-quinazolinone hybrids 14 were synthesized and tested the molecules for their cytotoxicity and Aurora-A kinase inhibitory activity by MTT assay [67]. All the tested hybrids showed good cytotoxicity activity, with IC50 values ranging from 0.38 to 18.13 M against three cancer cell lines namely breast cancer (MDA-MB-231), prostate cancer (PC3) and neuroblastoma (SH-SY5Y) ( Figure 17). Among them, the morpholinoethyl compound 14a displayed excellent activity with an IC50 value of 0.38 M (MDA-MB-231), 1.09 M (PC3) and 0.77 M (SH-SY5Y). Compound 14a also showed promising inhibitory activity (IC50 = 21.94 M) against Aurora-A kinase. In addition to, Benzoimidazole-quinazolinone hybrids 14 were synthesized and tested the molecules for their cytotoxicity and Aurora-A kinase inhibitory activity by MTT assay [67]. All the tested hybrids showed good cytotoxicity activity, with IC 50 values ranging from 0.38 to 18.13 µM against three cancer cell lines namely breast cancer (MDA-MB-231), prostate cancer (PC3) and neuroblastoma (SH-SY5Y) ( Figure 17). Among them, the morpholinoethyl compound 14a displayed excellent activity with an IC 50 value of 0.38 µM (MDA-MB-231), 1.09 µM (PC3) and 0.77 µM (SH-SY5Y). Compound 14a also showed promising inhibitory activity (IC 50 = 21.94 µM) against Aurora-A kinase. In addition to, compound 14a displayed induced G2/M phase cell cycle arrest and cell apoptosis via Aurora-A kinase inhibition. The replacement of the morpholinoethyl moiety with a methyl group might decrease the anticancer activity. Moreover, an extended propenamide alkyl side-chain at position 2 of the benzimidazole or an increased steric hindrance due to the introduction of a macrocyclic substituent may diminish the anticancer activity. Also, the replacement of propenamide with an electron-withdrawing group ethyl sulfonamide significantly reduced the anticancer activity. Furthermore, molecular docking studies revealed that the binding mode of compound 14a formed hydrogen bonding interactions with the amino acid residues Ala213 in the kinase hinge region, the main chain NH of Thr217 and the catalytic lysine of Lys162. The quinazolinone fragment formed hydrophobic interactions including π-π stacked and π-alkyl interactions with different residues including Leu139, Ala160, Leu194, Leu210, Ala213 and Leu263 of Aurora-A. The benzoimidazole-quinazoline hybrids are promising candidates for the progress of potential anticancer agents. compound 14a displayed induced G2/M phase cell cycle arrest and cell apoptosis via Aurora-A kinase inhibition. The replacement of the morpholinoethyl moiety with a methyl group might decrease the anticancer activity. Moreover, an extended propenamide alkyl side-chain at position 2 of the benzimidazole or an increased steric hindrance due to the introduction of a macrocyclic substituent may diminish the anticancer activity. Also, the replacement of propenamide with an electron-withdrawing group ethyl sulfonamide significantly reduced the anticancer activity. Furthermore, molecular docking studies revealed that the binding mode of compound 14a formed hydrogen bonding interactions with the amino acid residues Ala213 in the kinase hinge region, the main chain NH of Thr217 and the catalytic lysine of Lys162. The quinazolinone fragment formed hydrophobic interactions including π-π stacked and π-alkyl interactions with different residues including Leu139, Ala160, Leu194, Leu210, Ala213 and Leu263 of Aurora-A. The benzoimidazolequinazoline hybrids are promising candidates for the progress of potential anticancer agents. Molecules 15 having 1-substituted-2-arylimidazoles were synthesized and screened for their antiproliferative activity against six cancer cell lines, namely breast (MDA-MB-468, MDA-MB-231 and T47D), colorectal (HCT15 and HT-29), cervical (Hela) and endothelial (HUVEc) and for tubulin polymerization inhibition [68]. Among the tested derivatives, the one with a 3-NH2 and 4-OCH3 substituted phenyl ring (compound 15a) exhibited superior anticancer activity with IC50 values of 0.09 M against the MDA-MB-468 cell line and 0.08 M against the Hela cell line ( Figure 18). The active compound caused G2/M phase cell arrest and cell apoptosis and strongly inhibited tubulin polymerization. Furthermore, in vivo studies of compound 15a also demonstrated highly effective tumor growth inhibition of 77% at 60 mg/kg in an MDA-MB-468 cell. Introducing an electronwithdrawing group on the aromatic C-ring leads to a decrease in activity while an electron-donating group enhances the activity. Molecular docking studies of this compound 15a indicated that it bound well in the colchicine binding site of tubulin and a hydrogen bonding was observed between the amino group of 15a and the Thr179 of tubulin. These hydrogen bonds, together with the hydrophobic interactions provided by the aromatic rings, contribute to the high binding affinity of 15a to tubulin, resulting in high activity. The diarylimidazole derivatives are promising candidates for the development of potential anticancer agents. Molecules 15 having 1-substituted-2-arylimidazoles were synthesized and screened for their antiproliferative activity against six cancer cell lines, namely breast (MDA-MB-468, MDA-MB-231 and T47D), colorectal (HCT15 and HT-29), cervical (Hela) and endothelial (HUVEc) and for tubulin polymerization inhibition [68]. Among the tested derivatives, the one with a 3-NH 2 and 4-OCH 3 substituted phenyl ring (compound 15a) exhibited superior anticancer activity with IC 50 values of 0.09 µM against the MDA-MB-468 cell line and 0.08 µM against the Hela cell line ( Figure 18). The active compound caused G2/M phase cell arrest and cell apoptosis and strongly inhibited tubulin polymerization. Furthermore, in vivo studies of compound 15a also demonstrated highly effective tumor growth inhibition of 77% at 60 mg/kg in an MDA-MB-468 cell. Introducing an electron-withdrawing group on the aromatic C-ring leads to a decrease in activity while an electron-donating group enhances the activity. Molecular docking studies of this compound 15a indicated that it bound well in the colchicine binding site of tubulin and a hydrogen bonding was observed between the amino group of 15a and the Thr179 of tubulin. These hydrogen bonds, together with the hydrophobic interactions provided by the aromatic rings, contribute to the high binding affinity of 15a to tubulin, resulting in high activity. The diarylimidazole derivatives are promising candidates for the development of potential anticancer agents. Imidazole and its flavonoid analogs 16 were synthesized and the analogs evaluated for their protein tyrosine phosphatase1B (PTP1B) inhibitory activity as potential antidiabetic agents [69]. All the hybrids showed good PTP1B inhibitory activity with IC50 values ranging from 0.63-5.0 M, compared to oleanolic acid (IC50 = 4.7 M). Among the series, compound 16a bearing a 3-chloro group on the phenyl ring moiety exhibited potent PTP1B inhibitory activity with an IC50 value of 0.63 M (Figure 19). Compound 16a displayed a 9.5-fold high selectivity ratio for PTP1B over T-cell protein tyrosine phosphatase (TCPTP). Compound 16a also presented low toxicity to a normal human embryonic kidney (HEK293) cell line. Reduced activity was observed with the replacement of the chlorobenzyl moiety by a fluorobenzyl group. The positional change of chlorine atom on the phenyl ring was not beneficial for the bioactivity either. The OH group of compound 16a facilitated hydrogen bonding interactions with the Lys197 and Glu200 residues and also other hydrogen bonding interactions that formed through the nitrogen atoms of the imidazole ring with the Asn193 residue of PTP1B. The outcomes revealed that imidazole flavonoid scaffolds are potential inhibitors of PTP1B for the progress of antidiabetic agents. Series of benzo [1,2,5]thiadiazole-imidazole derivatives 17 were prepared and tested for their activin receptor-like kinase 5 (ALK5) inhibitory activity [70]. Among the series of compounds, the derivative 17a substituted with a meta-fluoro group on the phenyl ring exhibited excellent inhibitiory activity (IC50 = 0.008 M) against ALK5 kinase ( Figure 20). SAR studies revealed that the strong electron-donating (CH3) groups of the derivatives showed less activity than the halogen-substituted on the phenyl ring derivatives. In addition, compound 17a also strongly inhibited TGF-β1-induced Smad signaling in SPC-A1 and HepG2 cells. It showed however very low aqueous solubility, which Imidazole and its flavonoid analogs 16 were synthesized and the analogs evaluated for their protein tyrosine phosphatase1B (PTP1B) inhibitory activity as potential antidiabetic agents [69]. All the hybrids showed good PTP1B inhibitory activity with IC 50 values ranging from 0.63-5.0 µM, compared to oleanolic acid (IC 50 = 4.7 µM). Among the series, compound 16a bearing a 3-chloro group on the phenyl ring moiety exhibited potent PTP1B inhibitory activity with an IC 50 value of 0.63 µM (Figure 19). Compound 16a displayed a 9.5-fold high selectivity ratio for PTP1B over T-cell protein tyrosine phosphatase (TCPTP). Compound 16a also presented low toxicity to a normal human embryonic kidney (HEK293) cell line. Reduced activity was observed with the replacement of the chlorobenzyl moiety by a fluorobenzyl group. The positional change of chlorine atom on the phenyl ring was not beneficial for the bioactivity either. The OH group of compound 16a facilitated hydrogen bonding interactions with the Lys197 and Glu200 residues and also other hydrogen bonding interactions that formed through the nitrogen atoms of the imidazole ring with the Asn193 residue of PTP1B. The outcomes revealed that imidazole flavonoid scaffolds are potential inhibitors of PTP1B for the progress of antidiabetic agents. Imidazole and its flavonoid analogs 16 were synthesized and the analogs evaluated for their protein tyrosine phosphatase1B (PTP1B) inhibitory activity as potential antidiabetic agents [69]. All the hybrids showed good PTP1B inhibitory activity with IC50 values ranging from 0.63-5.0 M, compared to oleanolic acid (IC50 = 4.7 M). Among the series, compound 16a bearing a 3-chloro group on the phenyl ring moiety exhibited potent PTP1B inhibitory activity with an IC50 value of 0.63 M (Figure 19). Compound 16a displayed a 9.5-fold high selectivity ratio for PTP1B over T-cell protein tyrosine phosphatase (TCPTP). Compound 16a also presented low toxicity to a normal human embryonic kidney (HEK293) cell line. Reduced activity was observed with the replacement of the chlorobenzyl moiety by a fluorobenzyl group. The positional change of chlorine atom on the phenyl ring was not beneficial for the bioactivity either. The OH group of compound 16a facilitated hydrogen bonding interactions with the Lys197 and Glu200 residues and also other hydrogen bonding interactions that formed through the nitrogen atoms of the imidazole ring with the Asn193 residue of PTP1B. The outcomes revealed that imidazole flavonoid scaffolds are potential inhibitors of PTP1B for the progress of antidiabetic agents. Series of benzo [1,2,5]thiadiazole-imidazole derivatives 17 were prepared and tested for their activin receptor-like kinase 5 (ALK5) inhibitory activity [70]. Among the series of compounds, the derivative 17a substituted with a meta-fluoro group on the phenyl ring exhibited excellent inhibitiory activity (IC50 = 0.008 M) against ALK5 kinase ( Figure 20). SAR studies revealed that the strong electron-donating (CH3) groups of the derivatives showed less activity than the halogen-substituted on the phenyl ring derivatives. In addition, compound 17a also strongly inhibited TGF-β1-induced Smad signaling in SPC-A1 and HepG2 cells. It showed however very low aqueous solubility, which Series of benzo [1,2,5]thiadiazole-imidazole derivatives 17 were prepared and tested for their activin receptor-like kinase 5 (ALK5) inhibitory activity [70]. Among the series of compounds, the derivative 17a substituted with a meta-fluoro group on the phenyl ring exhibited excellent inhibitiory activity (IC 50 = 0.008 µM) against ALK5 kinase ( Figure 20). SAR studies revealed that the strong electron-donating (CH 3 ) groups of the derivatives showed less activity than the halogen-substituted on the phenyl ring derivatives. In addition, compound 17a also strongly inhibited TGF-β1-induced Smad signaling in SPC-A1 and HepG2 cells. It showed however very low aqueous solubility, which limits oral administration, and its structure contains pyridine and imidazole moieties that can form salts in stomach acids, which can improve the water solubility. Based on the ADMET analysis the active compound 17a showed good pharmacokinetic properties.
Molecules 2020, 25, x FOR PEER REVIEW 18 of 44 limits oral administration, and its structure contains pyridine and imidazole moieties that can form salts in stomach acids, which can improve the water solubility. Based on the ADMET analysis the active compound 17a showed good pharmacokinetic properties. A library of 1,6-disubstituted-1H-benzo[d]imidazole derivatives 18 was synthesized and the derivatives tested for their in vitro antiproliferative activity against the T47D, HCT116 and MCF-7 cancer cell lines [71]. Among them, the compound 18a with a 2,4-difluoro substitution pattern on the sulfonyl phenyl ring showed significant activity, with IC50 values of 0.36, 0.14 and 0.31 M against the T47D, HCT116 and MCF-7 cancer cell lines, respectively ( Figure 21). SAR studies revealed that the difluoro-substituted derivatives have superior antiproliferative activity than the methyl-substituted derivatives. Introducing a methoxy group led to a decrease in the activity. In addition, the active compound 18a effectively inhibited cell proliferation through suppression of PI3K kinase and blocking the PI3K/Akt pathway in HCT116 cells. Additionally, 18a could inhibit the migration and invasion ability of HCT116 cells and could induce apoptosis of HCT116 cells. Molecular docking studies of this molecule 18a could fit into the binding site of PI3K kinase. The nitrogen of the benzo[d]imidazole group formed a hydrogen bonding with the side-chain  A library of 1,6-disubstituted-1H-benzo[d]imidazole derivatives 18 was synthesized and the derivatives tested for their in vitro antiproliferative activity against the T47D, HCT116 and MCF-7 cancer cell lines [71]. Among them, the compound 18a with a 2,4-difluoro substitution pattern on the sulfonyl phenyl ring showed significant activity, with IC50 values of 0.36, 0.14 and 0.31 M against the T47D, HCT116 and MCF-7 cancer cell lines, respectively ( Figure 21). SAR studies revealed that the difluoro-substituted derivatives have superior antiproliferative activity than the methyl-substituted derivatives. Introducing a methoxy group led to a decrease in the activity. In addition, the active compound 18a effectively inhibited cell proliferation through suppression of PI3K kinase and blocking the PI3K/Akt pathway in HCT116 cells. Additionally, 18a could inhibit the migration and invasion ability of HCT116 cells and could induce apoptosis of HCT116 cells. Molecular docking studies of this molecule 18a could fit into the binding site of PI3K kinase. The nitrogen of the benzo[d]imidazole group formed a hydrogen bonding with the side-chain of Val882 in the hinge binder region of PI3K. Furthermore, the oxygen of the methoxy group formed SAR studies revealed that the difluoro-substituted derivatives have superior antiproliferative activity than the methyl-substituted derivatives. Introducing a methoxy group led to a decrease in the activity. In addition, the active compound 18a effectively inhibited cell proliferation through suppression of PI3K kinase and blocking the PI3K/Akt pathway in HCT116 cells. Additionally, 18a could inhibit the migration and invasion ability of HCT116 cells and could induce apoptosis of HCT116 cells. Molecular docking studies of this molecule 18a could fit into the binding site of PI3K kinase. The nitrogen of the benzo[d]imidazole group formed a hydrogen bonding with the side-chain of Val882 in the hinge binder region of PI3K. Furthermore, the oxygen of the methoxy group formed an additional hydrogen bonding interaction with Lys833. Moreover, the nitrogen of the pyridyl group formed a hydrogen bonding with the conserved water molecule. In addition to these, the hydrogen of the hydroxyl group formed a hydrogen bonding interaction with Thr887. As evident from the ADMET investigation, compound 18a displayed good drug-like properties. Thus, the benzoimidazole derivatives are promising candidates as potential PI3K inhibitors for anticancer drug advances.
Imidazole tethered pyrazole hybrids 19 were synthesized and the hybrids screened for their in vitro α-glucosidase enzyme inhibitory activity as potential antidiabetic agents [72]. The two hybrids with a bromine atom at the para-position of the phenyl ring attached to the imidazole nitrogen (compounds 19a and 19b) displayed the highest inhibitory activity with IC 50 values of 25.19 µM and 33.62 µM against the α-glucosidase enzyme, respectively, as compared with the standard acarbose (IC 50 = 38.25 µM, Figure 22). SAR studies revealed that the halogen (Br and Cl)-substituted hybrids have superior inhibitory activity than those with electron-donating groups substituents. Molecular docking studies of the molecules 19a and 19b showed that they interact with the binding pocket of the α-glucosidase enzyme, and a hydrogen bonding interaction was observed between the unsubstituted nitrogen atom of pyrazole ring and amino acid Asn24. Moreover, the oxygen atoms of the nitro group were acting as hydrogen bonding acceptors towards the amino acid Arg312. an additional hydrogen bonding interaction with Lys833. Moreover, the nitrogen of the pyridyl group formed a hydrogen bonding with the conserved water molecule. In addition to these, the hydrogen of the hydroxyl group formed a hydrogen bonding interaction with Thr887. As evident from the ADMET investigation, compound 18a displayed good drug-like properties. Thus, the benzoimidazole derivatives are promising candidates as potential PI3K inhibitors for anticancer drug advances. Imidazole tethered pyrazole hybrids 19 were synthesized and the hybrids screened for their in vitro α-glucosidase enzyme inhibitory activity as potential antidiabetic agents [72]. The two hybrids with a bromine atom at the para-position of the phenyl ring attached to the imidazole nitrogen (compounds 19a and 19b) displayed the highest inhibitory activity with IC50 values of 25.19 M and 33.62 M against the α-glucosidase enzyme, respectively, as compared with the standard acarbose (IC50 = 38.25 M, Figure 22). SAR studies revealed that the halogen (Br and Cl)-substituted hybrids have superior inhibitory activity than those with electron-donating groups substituents. Molecular docking studies of the molecules 19a and 19b showed that they interact with the binding pocket of the α-glucosidase enzyme, and a hydrogen bonding interaction was observed between the unsubstituted nitrogen atom of pyrazole ring and amino acid Asn24. Moreover, the oxygen atoms of the nitro group were acting as hydrogen bonding acceptors towards the amino acid Arg312.

Pyrazoles
Pyrazole is a well-known five-membered nitrogen-based heterocycle and exhibits a broad spectrum of synthetic and biological applications. Several pyrazole-based drugs namely celecoxib, rimonabant, difenamizole and fezolamine, etc., with excellent anti-inflammatory, anti-obesity, analgesic and/or antidepressant activities have been developed and are used to treat various diseases ( Figure 23) [32][33][34][35].

Pyrazoles
Pyrazole is a well-known five-membered nitrogen-based heterocycle and exhibits a broad spectrum of synthetic and biological applications. Several pyrazole-based drugs namely celecoxib, rimonabant, difenamizole and fezolamine, etc., with excellent anti-inflammatory, anti-obesity, analgesic and/or antidepressant activities have been developed and are used to treat various diseases ( Figure 23) [32][33][34][35].

Pyrazoles
Pyrazole is a well-known five-membered nitrogen-based heterocycle and exhibits a broad spectrum of synthetic and biological applications. Several pyrazole-based drugs namely celecoxib, rimonabant, difenamizole and fezolamine, etc., with excellent anti-inflammatory, anti-obesity, analgesic and/or antidepressant activities have been developed and are used to treat various diseases ( Figure 23) [32][33][34][35].  Pyrazole fused triazole molecules 20 were identified as anti-inflammatory agents and COX-1/COX-2 enzyme inhibitors by Tageldin and co-workers [73]. Among them, compound 20a showed promising anti-inflammatory activity with an IC 50 value of 4.33 µM against the COX-1 enzyme as compared with celecoxib (IC 50 = 5.46 µM) ( Figure 24). SAR studies revealed that the less activity was observed with alkyl substituent derivatives than the acetoxy derivatives. Furthermore, docking studies of the most active compound showed that the acetate moiety of 20a was involved in a H-bonding interaction with the Tyr385 of the COX-2 enzyme. In addition, the 1-phenyl substituent attached to the core pyrazolo [4,3- Pyrazole fused triazole molecules 20 were identified as anti-inflammatory agents and COX-1/COX-2 enzyme inhibitors by Tageldin and co-workers [73]. Among them, compound 20a showed promising anti-inflammatory activity with an IC50 value of 4.33 M against the COX-1 enzyme as compared with celecoxib (IC50 = 5.46 M) ( Figure 24). SAR studies revealed that the less activity was observed with alkyl substituent derivatives than the acetoxy derivatives. Furthermore, docking studies of the most active compound showed that the acetate moiety of 20a was involved in a Hbonding interaction with the Tyr385 of the COX-2 enzyme. In addition, the 1-phenyl substituent attached to the core pyrazolo [4,3-   Pyrazole sulfonamide conjugates 21 were prepared and evaluated for their in vitro analgesic and dual COX-2/5-LOX inhibitory activity [74]. The pyrazole derivative with a benzothiophene (conmpound 21a) exhibited potent inhibitory activity with the IC 50 values of 5.40 µM (COX-1), 0.014 µM (COX-2) and 1.78 µM (5-LOX) ( Figure 25). Compound 21a showed equal or more potent analgesic activity (88.8% protection) than the standards indomethacin (84.4%) and celecoxib (31.1%), with no gastric ulcerogenic properties.
Furthermore, the binding mode of the active compound 21a was evaluated by molecular docking studies, where the negatively charged carboxylate moiety at the position 3 of the pyrazole compound was shown to be anchored in the active site through ionic interaction with the positively charged non-heme iron. Through hydrophobic interaction, the aryl substituent at position 5 interacts with the hydrophobic side-chains of the amino acids Leu368, Leu414, Ile415 and Phe421. In addition, the aryl substituent and the benzenesulfonamide moieties are involved in Van der Waals interactions with the amino acid Phe421. Through hydrogen bonding, the sulfonamide moiety interacts with Tyr181 and Asn425 residues in 5-LOX enzyme.
Pyrazole sulfonamide conjugates 21 were prepared and evaluated for their in vitro analgesic and dual COX-2/5-LOX inhibitory activity [74]. The pyrazole derivative with a benzothiophene (conmpound 21a) exhibited potent inhibitory activity with the IC50 values of 5.40 M (COX-1), 0.014 M (COX-2) and 1.78 M (5-LOX) ( Figure 25). Compound 21a showed equal or more potent analgesic activity (88.8% protection) than the standards indomethacin (84.4%) and celecoxib (31.1%), with no gastric ulcerogenic properties. Furthermore, the binding mode of the active compound 21a was evaluated by molecular docking studies, where the negatively charged carboxylate moiety at the position 3 of the pyrazole compound was shown to be anchored in the active site through ionic interaction with the positively charged non-heme iron. Through hydrophobic interaction, the aryl substituent at position 5 interacts with the hydrophobic side-chains of the amino acids Leu368, Leu414, Ile415 and Phe421. In addition,  [1,2,5]thiadiazol-5-yl moiety formed a π-alkyl bonding with Leu340. Lys232 formed a π-alkyl bonding with the pyridine ring in 22a and the benzene ring of Tyr249 formed a π-alkyl bonding with the methyl group of the pyridine ring. The NH group of the side-chain in 22a formed a hydrogen bonding with the carbonyl group of the Lys337 and Asn338, and with the carboxylic acid of the side chain in Asp351. The phenyl ring of the side-chain formed a π-alkyl bonding with the Lys337 residue.  [1,2,5]thiadiazol-5-yl moiety formed a π-alkyl bonding with Leu340. Lys232 formed a π-alkyl bonding with the pyridine ring in 22a and the benzene ring of Tyr249 formed a πalkyl bonding with the methyl group of the pyridine ring. The NH group of the side-chain in 22a formed a hydrogen bonding with the carbonyl group of the Lys337 and Asn338, and with the carboxylic acid of the side chain in Asp351. The phenyl ring of the side-chain formed a π-alkyl bonding with the Lys337 residue. Pyrazole-linked thiohydantoin derivatives 23 were synthesized and tested for their anti-cancer and anti-inflammatory activities [76]. Among them, the nitro substituted compound 23a showed high anti-inflammatory activity (IC50 = 0.65 M) against the COX-2 enzyme as compared with celecoxib (IC50 = 0.84 M) (Figure 27). The compound exhibited significantly less ulcerogenic activity (ulcer index = 3.21) than the non-ulcerogenic celecoxib as the reference drug (ulcer index = 2.99). In addition to that, a series of compounds were evaluated for their cytotoxic activity against lung carcinoma (A-549), colon (HCT-116) and breast (MCF-7) cancer cell lines. The same compound 23a exhibited the Pyrazole-linked thiohydantoin derivatives 23 were synthesized and tested for their anti-cancer and anti-inflammatory activities [76]. Among them, the nitro substituted compound 23a showed high anti-inflammatory activity (IC 50 = 0.65 µM) against the COX-2 enzyme as compared with celecoxib  (Figure 27). The compound exhibited significantly less ulcerogenic activity (ulcer index = 3.21) than the non-ulcerogenic celecoxib as the reference drug (ulcer index = 2.99). In addition to that, a series of compounds were evaluated for their cytotoxic activity against lung carcinoma (A-549), colon (HCT-116) and breast (MCF-7) cancer cell lines. The same compound 23a exhibited the most potent cytotoxic activity (IC 50 = 3.73 µM) against the human colon (HCT-116) cancer cell line. Compound 23a inhibited human topoisomerase-1 (Topo-1) with an IC 50 value of 29.7 µg/mL, as compared with the reference camptothecin (IC 50 = 20.2 µg/mL). The methoxy-substituted derivative has higher cytotoxic activity than the respective unsubstituted derivatives. Furthermore, the binding mode of the active compound 23a with COX-2 and human topoisomerase-1 enzyme was investigated by docking studies. Compound 23a interacts via hydrogen bonding with Tyr341, Tyr371, Ser516 residues of COX-2. Also, there is a hydrogen bonding interaction with the Asp533 and two π-π stacking interactions with DNA base pairs through arene-arene interactions with deoxyadenosine DA113 of the human topoisomerase-1 enzyme. It can be concluded that for these types of analogs, the presence of a para-substituted methoxy in addition to the COX-2 pharmacophore SO 2 CH 3 is important for binding with both COX-2 and human Topo-1. The pyrazole-thiohydantoin derivatives are promising candidates for the development of anti-inflammatory and anti-cancer agents.  Pyrazole-tagged benzothiazole-β-naphthol hybrids 24 were prepared and evaluated for their in vitro antiproliferative activity against four (A549, MCF 7, HeLa and HEK-293) human cancer cell lines [77]. Amongst them, compound 24a bearing a fluorine atom at the para-position on the phenyl ring moiety showed the most significant antiproliferative activity against the human epithelial cervical (HeLa) cancer cell line (IC50 = 4.63 M) ( Figure 28). SAR studies revealed that the compound with electron-withdrawing groups exhibited greater activity than those with electron-donating groups. Compound 24a caused G2/M phase cell cycle arrest and effectively inhibited Topo-1 and was shown to strongly bind to DNA. Furthermore, docking studies showed that the compound significantly interacts with topoisomerase-I and DNA by binding to its minor groove. Pyrazole-tagged benzothiazole-β-naphthol hybrids 24 were prepared and evaluated for their in vitro antiproliferative activity against four (A549, MCF 7, HeLa and HEK-293) human cancer cell lines [77]. Amongst them, compound 24a bearing a fluorine atom at the para-position on the phenyl ring moiety showed the most significant antiproliferative activity against the human epithelial cervical (HeLa) cancer cell line (IC 50 = 4.63 µM) ( Figure 28). SAR studies revealed that the compound with electron-withdrawing groups exhibited greater activity than those with electron-donating groups. Compound 24a caused G2/M phase cell cycle arrest and effectively inhibited Topo-1 and was shown to strongly bind to DNA. Furthermore, docking studies showed that the compound significantly interacts with topoisomerase-I and DNA by binding to its minor groove. [77]. Amongst them, compound 24a bearing a fluorine atom at the para-position on the phenyl ring moiety showed the most significant antiproliferative activity against the human epithelial cervical (HeLa) cancer cell line (IC50 = 4.63 M) ( Figure 28). SAR studies revealed that the compound with electron-withdrawing groups exhibited greater activity than those with electron-donating groups. Compound 24a caused G2/M phase cell cycle arrest and effectively inhibited Topo-1 and was shown to strongly bind to DNA. Furthermore, docking studies showed that the compound significantly interacts with topoisomerase-I and DNA by binding to its minor groove.  Figure  29). SAR studies revealed that the dichloro and bromo groups substituted on the phenyl ring derivatives were inactive. Furthermore, the active compound 25a exhibited low cytotoxicity against  Figure 29). SAR studies revealed that the dichloro and bromo groups substituted on the phenyl ring derivatives were inactive. Furthermore, the active compound 25a exhibited low cytotoxicity against the human HeLa, PANC-1 and A549 cancer cells. These hybrids are potential candidates for the progress of antitubercular agents.  Pyrazole and its 1,3-diphenyl analogs 26 were synthesized and the new molecules tested for their protein tyrosine phosphatase1B (PTP1B) inhibitory activity [79]. All the tested compounds exhibited good inhibitory activity with IC50 values ranging from 0.67-24.56 M (Figure 30). The 2,4dichloro with butyl linker derivative 26a exhibited superior inhibitory activity (IC50 = 0.67 M) against the PTP1B enzyme as compared to the standard oleanolic acid (IC50 = 1.62 M). The change in substitution on the phenyl ring and linker length influenced the activity. No activity was found for the fluoro-and methoxy-substituted on the phenyl ring molecules and a linker length of four. In addition, a 9-fold selectivity was observed against PTP1B over TCPTP enzymes. Furthermore, the active compound 26a was evaluated by the molecular docking studies and was fitted well into the catalytic site of the PTP1B enzyme. The carbonyl and hydroxyl moieties of the carboxyl groups that are known as hydrogen bonding acceptors and donors, formed three hydrogen bonding with the backbone of Ser216 and the side-chains of Arg221 and Cys215 in PTP1B. Some van der Waals interactions, such as interactions between the terminal benzene ring and Gly259 and Arg24, the sulfur atom of the carbon-sulfur double bond of the thiazole ring and Met258, and the sulfur atom of the thiazole ring and Asp48 residues in PTP1B were also observed. Pyrazole and its 1,3-diphenyl analogs 26 were synthesized and the new molecules tested for their protein tyrosine phosphatase1B (PTP1B) inhibitory activity [79]. All the tested compounds exhibited good inhibitory activity with IC 50 values ranging from 0.67-24.56 µM (Figure 30). The 2,4-dichloro with butyl linker derivative 26a exhibited superior inhibitory activity (IC 50 = 0.67 µM) against the PTP1B enzyme as compared to the standard oleanolic acid (IC 50 = 1.62 µM). The change in substitution on the phenyl ring and linker length influenced the activity. No activity was found for the fluoro-and methoxy-substituted on the phenyl ring molecules and a linker length of four. In addition, a 9-fold selectivity was observed against PTP1B over TCPTP enzymes. Furthermore, the active compound 26a was evaluated by the molecular docking studies and was fitted well into the catalytic site of the PTP1B enzyme. The carbonyl and hydroxyl moieties of the carboxyl groups that are known as hydrogen bonding acceptors and donors, formed three hydrogen bonding with the backbone of Ser216 and the side-chains of Arg221 and Cys215 in PTP1B. Some van der Waals interactions, such as interactions between the terminal benzene ring and Gly259 and Arg24, the sulfur atom of the carbon-sulfur double bond of the thiazole ring and Met258, and the sulfur atom of the thiazole ring and Asp48 residues in PTP1B were also observed.
the PTP1B enzyme as compared to the standard oleanolic acid (IC50 = 1.62 M). The change in substitution on the phenyl ring and linker length influenced the activity. No activity was found for the fluoro-and methoxy-substituted on the phenyl ring molecules and a linker length of four. In addition, a 9-fold selectivity was observed against PTP1B over TCPTP enzymes. Furthermore, the active compound 26a was evaluated by the molecular docking studies and was fitted well into the catalytic site of the PTP1B enzyme. The carbonyl and hydroxyl moieties of the carboxyl groups that are known as hydrogen bonding acceptors and donors, formed three hydrogen bonding with the backbone of Ser216 and the side-chains of Arg221 and Cys215 in PTP1B. Some van der Waals interactions, such as interactions between the terminal benzene ring and Gly259 and Arg24, the sulfur atom of the carbon-sulfur double bond of the thiazole ring and Met258, and the sulfur atom of the thiazole ring and Asp48 residues in PTP1B were also observed.  Pyrazole derivatives 27 were synthesized and screened for their KDM5B inhibitory activity as potential gastric cancer agents [80]. Among the tested derivatives, the 4-methoxylbenzyl-substituted compound 27a exhibited significantly increased potency against KDM5B with an IC 50 of 24.4 nM ( Figure 31). The presence of an electron-donating 2-methoxybenzyl group decreased the activity compared with the 4-methoxy derivative. The para-substituted derivatives showed greater potency than the ortho-substituted derivatives. Due to the p-methoxyphenyl group in the junction of the hydrophobic and hydrophilic pocket, the phenyl was surrounded by Tyr488, Tyr425, and Val489 residues. In addition, 27a was a potent KDM5B inhibitor with acceptable selectivity against KDM5A/B/C, but it performed poorly against KDM4A/C and KDM6B. The capability of compound 27a to stabilize KDM5B in a dose-dependent manner and induce the accumulation of H3K4me2/3 in the gastric cancer cell line MKN45, supports the use of compound 27a as a potent and cellular active KDM5B inhibitor. Furthermore, the compound showed potent cellular active KDM5B inhibition and inhibited MKN45 cell proliferation, wound healing and migration. The molecular docking studies showed that molecule 27a fitted well in the tight flat binding pocket of KDM5B. The oxygen atom of the methoxy group formed a hydrogen bonding with the Lys517, and the nitrogen of amide had a hydrogen interaction with Tyr488 and π-π stacking interactions with the benzene ring of Tyr488 residues of KDM5B. The pyrazole derivatives are promising candidates for targeting KDM5B inhibition and as a new therapeutic strategy for gastric cancer treatment. Pyrazole derivatives 27 were synthesized and screened for their KDM5B inhibitory activity as potential gastric cancer agents [80]. Among the tested derivatives, the 4-methoxylbenzyl-substituted compound 27a exhibited significantly increased potency against KDM5B with an IC50 of 24.4 nM ( Figure 31). The presence of an electron-donating 2-methoxybenzyl group decreased the activity compared with the 4-methoxy derivative. The para-substituted derivatives showed greater potency than the ortho-substituted derivatives. Due to the p-methoxyphenyl group in the junction of the hydrophobic and hydrophilic pocket, the phenyl was surrounded by Tyr488, Tyr425, and Val489 residues. In addition, 27a was a potent KDM5B inhibitor with acceptable selectivity against KDM5A/B/C, but it performed poorly against KDM4A/C and KDM6B. The capability of compound 27a to stabilize KDM5B in a dose-dependent manner and induce the accumulation of H3K4me2/3 in the gastric cancer cell line MKN45, supports the use of compound 27a as a potent and cellular active KDM5B inhibitor. Furthermore, the compound showed potent cellular active KDM5B inhibition and inhibited MKN45 cell proliferation, wound healing and migration. The molecular docking studies showed that molecule 27a fitted well in the tight flat binding pocket of KDM5B. The oxygen atom of the methoxy group formed a hydrogen bonding with the Lys517, and the nitrogen of amide had a hydrogen interaction with Tyr488 and π-π stacking interactions with the benzene ring of Tyr488 residues of KDM5B. The pyrazole derivatives are promising candidates for targeting KDM5B inhibition and as a new therapeutic strategy for gastric cancer treatment. Pyrazole scaffolds 28 were synthesized and the scaffolds evaluated for their reactive oxygen species (ROS) inhibitory activity on human platelets [81]. Most of the tested compounds were able to inhibit ROS production with IC50 values of 8.6 to 38.3 M. Among the established molecules, the O- Pyrazole scaffolds 28 were synthesized and the scaffolds evaluated for their reactive oxygen species (ROS) inhibitory activity on human platelets [81]. Most of the tested compounds were able to inhibit ROS production with IC 50 values of 8.6 to 38.3 µM. Among the established molecules, the O-cyclopentyl and O-CH 3 compound 28a showed the most active ROS production inhibition with an IC 50 value of 38.3 µM (Figure 32). Pyrazole scaffolds 28 were synthesized and the scaffolds evaluated for their reactive oxygen species (ROS) inhibitory activity on human platelets [81]. Most of the tested compounds were able to inhibit ROS production with IC50 values of 8.6 to 38.3 M. Among the established molecules, the Ocyclopentyl and O-CH3 compound 28a showed the most active ROS production inhibition with an IC50 value of 38.3 M (Figure 32). Reduced activity was observed with a tri-methoxy substituted on the phenyl ring derivative than for all the tested compounds. In addition, compound 28a strongly inhibited both PDE4D3 (IC50 Reduced activity was observed with a tri-methoxy substituted on the phenyl ring derivative than for all the tested compounds. In addition, compound 28a strongly inhibited both PDE4D3 (IC 50 = 1.05 µM) and PDE4B2 (IC 50 = 0.55 µM) enzyme isomers. The results revealed that the pyrazole derivatives could block ROS production on human platelets, that produce high levels of ROS upon thrombin stimulation by a PDE4-independent mechanism.

Quinolines
The quinoline moiety is a well-known entity and is a ubiquitous alkaloid subunit of many natural products. Quinoline is an important pharmacophore moiety as it has been described to possess various biological activities, which include antimalarial, antibiotic, antitubercular, antiproliferative, antiprotozoal, antihypertensive and anti-HIV properties [82][83][84]. Currently various clinical drugs available in the market contain this moiety ( Figure 33).

Quinolines
The quinoline moiety is a well-known entity and is a ubiquitous alkaloid subunit of many natural products. Quinoline is an important pharmacophore moiety as it has been described to possess various biological activities, which include antimalarial, antibiotic, antitubercular, antiproliferative, antiprotozoal, antihypertensive and anti-HIV properties [82][83][84]. Currently various clinical drugs available in the market contain this moiety ( Figure 33). Quinoline conjugates 29 were synthesized and evaluated for their antiproliferative activity [85]. Among them, the compound 29a with a meta-methoxy on the phenyl ring and 3-phenylpropoxyl group exhibited the high antiproliferative activity against colorectal carcinoma (HCT-116) and cervical carcinoma (Hela) with IC50 values of 2.56 M and 2.71 M respectively (Figure 34). Compound 29a was strongly inhibited tumor growth (82.1%) in mice bearing CT25-C126 cells and Quinoline conjugates 29 were synthesized and evaluated for their antiproliferative activity [85]. Among them, the compound 29a with a meta-methoxy on the phenyl ring and 3-phenylpropoxyl group exhibited the high antiproliferative activity against colorectal carcinoma (HCT-116) and cervical carcinoma (Hela) with IC 50 values of 2.56 µM and 2.71 µM respectively ( Figure 34). Compound 29a was strongly inhibited tumor growth (82.1%) in mice bearing CT25-C126 cells and induced autophagy in colon cancer cells. It can be concluded that a large and bulky substituent in position-7 might be an advisable pharmacophoric group for antiproliferative activities. The novel quinoline analogs are potential candidates for the progress of antitumor agents.
Molecules 2020, 25   Tetrahydrobenzo-quinoline scaffolds 31 were prepared and tested for their antiproliferation activity against the four human cancer cell lines MCF-7, C26, A2780 and A549 [87]. The compound bearning with a p-methyl group on the phenyl ring (31a) showed the most significant antiproliferative activity against the human breast cancer cell line (MCF-7) with an IC50 value of the 1.51 M, as compared with the standard doxorubicin (IC50 = 0.5 M) (Figure 36). The compounds possessing small lipophilic electron-donating substituents in the para-position of the phenyl ring showed higher cytotoxic activity than the other quinolines. The pyridine derivatives did not show any improved cytotoxic effect, which can be due to the electron-withdrawing character of the pyridine ring. Compound 31a displayed significant DNA intercalating effects and effectively induced apoptosis in a dose-dependent manner. Furthermore, the authors undertook by molecular docking studies of the active compound 31a, and the interaction of this compound with nucleobases involved hydrogen bonding between the NH groups of compound 31a and adenine of the DNA backbone. The phenyl ring of compound 31a showed hydrophobic and Vander Waals interactions with two thymine groups of DNA.  Figure 35). The active compound 30a effectively induced p53-dependent cell apoptosis by targeting p53 transcriptional activity and reduced the viability of HCT116, DLD1 and RKO cells in a time-dependent manner in the CCK8 assay. In addition, compound 30a significantly inhibited tumor growth in a colorectal cancer xenograft model in nude mice. The compounds bearing a flexible amino side-chain with a two-methylene spacer displayed more potent cytotoxic potency than compounds that contained a three-methylene spacer. The results indicated that the amino side-chain substituents are beneficial pharmacophoric groups for enhancing the antiproliferative activity. Thus, quinoline scaffolds are potential compounds for the progress of antitumor agents.   Tetrahydrobenzo-quinoline scaffolds 31 were prepared and tested for their antiproliferation activity against the four human cancer cell lines MCF-7, C26, A2780 and A549 [87]. The compound bearning with a p-methyl group on the phenyl ring (31a) showed the most significant antiproliferative activity against the human breast cancer cell line (MCF-7) with an IC50 value of the 1.51 M, as Tetrahydrobenzo-quinoline scaffolds 31 were prepared and tested for their antiproliferation activity against the four human cancer cell lines MCF-7, C26, A2780 and A549 [87]. The compound bearning with a p-methyl group on the phenyl ring (31a) showed the most significant antiproliferative activity against the human breast cancer cell line (MCF-7) with an IC 50 value of the 1.51 µM, as compared with the standard doxorubicin (IC 50 = 0.5 µM) (Figure 36). The compounds possessing small lipophilic electron-donating substituents in the para-position of the phenyl ring showed higher cytotoxic activity than the other quinolines. The pyridine derivatives did not show any improved cytotoxic effect, which can be due to the electron-withdrawing character of the pyridine ring. Compound 31a displayed significant DNA intercalating effects and effectively induced apoptosis in a dose-dependent manner. Furthermore, the authors undertook by molecular docking studies of the active compound 31a, and the interaction of this compound with nucleobases involved hydrogen bonding between the NH groups of compound 31a and adenine of the DNA backbone. The phenyl ring of compound 31a showed hydrophobic and Vander Waals interactions with two thymine groups of DNA. Quinoline-indole-hybrids 32 were synthesized and tested for antiproliferative activity [88]. Compound 32a with a hydroxymethyl on the indole ring showed the most potent activity with an IC50 value of 2 nM against K562 cancer cells ( Figure 37). Compound 32a caused cell cycle arrest at the G2/M phase and induced apoptosis. In addition, 32a effectively inhibited tumor growth in H22 xenograft models with no toxicity. Furthermore, compound 32a binds well into the colchicine binding site of tubulin. The N-1 of the quinoline moiety formed hydrogen bonding with the residue of Cys241 and the indole rings extended into the hydrophobic pocket, which was surrounded by the residues Thr179, Val315, Asn350 and Val351. The hydroxymethyl group of 32a formed two additional hydrogen bonds with Val315 and Asn350 residues. The novel quinoline hybrids are potential hybrids for the development of anti-tubulin agents.  Quinoline-indole-hybrids 32 were synthesized and tested for antiproliferative activity [88]. Compound 32a with a hydroxymethyl on the indole ring showed the most potent activity with an IC 50 value of 2 nM against K562 cancer cells ( Figure 37). Compound 32a caused cell cycle arrest at the G2/M phase and induced apoptosis. In addition, 32a effectively inhibited tumor growth in H22 xenograft models with no toxicity. Furthermore, compound 32a binds well into the colchicine binding site of tubulin. The N-1 of the quinoline moiety formed hydrogen bonding with the residue of Cys241 and the indole rings extended into the hydrophobic pocket, which was surrounded by the residues Thr179, Val315, Asn350 and Val351. The hydroxymethyl group of 32a formed two additional hydrogen bonds with Val315 and Asn350 residues. The novel quinoline hybrids are potential hybrids for the development of anti-tubulin agents. Quinoline-indole-hybrids 32 were synthesized and tested for antiproliferative activity [88]. Compound 32a with a hydroxymethyl on the indole ring showed the most potent activity with an IC50 value of 2 nM against K562 cancer cells ( Figure 37). Compound 32a caused cell cycle arrest at the G2/M phase and induced apoptosis. In addition, 32a effectively inhibited tumor growth in H22 xenograft models with no toxicity. Furthermore, compound 32a binds well into the colchicine binding site of tubulin. The N-1 of the quinoline moiety formed hydrogen bonding with the residue of Cys241 and the indole rings extended into the hydrophobic pocket, which was surrounded by the residues Thr179, Val315, Asn350 and Val351. The hydroxymethyl group of 32a formed two additional hydrogen bonds with Val315 and Asn350 residues. The novel quinoline hybrids are potential hybrids for the development of anti-tubulin agents. Quinoline hybrids 33 with a 1,2,3-triazole linker were synthesized and the new molecules screened for their antitubercular activity against Mycobacterium bovis [89]. Among the series of hybrids, compound 33a bearing a 3-fluoro on the phenyl ring showed the most potent antitubercular activity with a MIC value of 31.35 µM, as compared to the standard isoniazid (MIC = 12.52 µM) (Figure 38). The presence of fluorine on the meta-position of the phenyl ring showed superior activity, whereas orthoand para-fluoro phenyl did not display any activity. Furthermore, these hybrids tested for cytotoxicity and the active compound 33a showed no growth inhibition for all the tested cell lines HeLa (cervical), PC3 (prostate), Panc-1 (pancreatic) and SKOV3 (ovarian). Schiff bases of quinoline analogs 34 were synthesized and tested for their anti-diabetic activity against the α-glucosidase enzyme [90]. All the screened molecules exhibited good inhibitory activity with IC50 values ranging from 6.20-48.50 M. Among them, the 3,4-dihydroxy conjugate 34a displayed potent inhibitory activity (IC50 = 6.20 M) against the α-glucosidase enzyme ( Figure 39). The active compound 34a showed a 6-fold greater potency than the reference acarbose (IC50 = 38.45 M). SAR studies revealed that no inhibitory activity was noticed in the presence of electron-donating groups. The position of the hydroxyl moiety plays a role in the activity of these compounds, the relocation of OH groups from 3 and 4-position and their respective presence at the phenyl ring 2,5and 2,3-positions decreased the inhibitory activity and showed lower inhibitory potential than analog 34a. The hydroxyl moieties present in the para-position of the phenyl ring showed more interactions with the active site residues. The methoxy moiety instead of the hydroxyl moiety showed fewer interactions with active site residues as well as less activity. Furthermore, the docking studies of compound 34a validated that the oxygen atom of the hydroxyl moiety of the phenyl ring of the compound formed a hydrogen bonding interaction with the active site residues of Lys126 and Glu171 of the α-glucosidase enzyme. The quinoline-Schiff-based derivative is a potential inhibitory agent for the treatment of anti-diabetic activity. Hybrid molecules 35 having quinoline, pyrazole and thiazole moieties were synthesized and evaluated for their antiproliferative activity [91]. Among them, the hybrid 35a with a para-fluoro substituent on the phenyl ring showed excellent antiproliferative activity (IC50 = 0.136 M) against Schiff bases of quinoline analogs 34 were synthesized and tested for their anti-diabetic activity against the α-glucosidase enzyme [90]. All the screened molecules exhibited good inhibitory activity with IC 50 values ranging from 6.20-48.50 µM. Among them, the 3,4-dihydroxy conjugate 34a displayed potent inhibitory activity (IC 50 = 6.20 µM) against the α-glucosidase enzyme ( Figure 39). The active compound 34a showed a 6-fold greater potency than the reference acarbose (IC 50 = 38.45 µM). SAR studies revealed that no inhibitory activity was noticed in the presence of electron-donating groups. The position of the hydroxyl moiety plays a role in the activity of these compounds, the relocation of OH groups from 3 and 4-position and their respective presence at the phenyl ring 2,5-and 2,3-positions decreased the inhibitory activity and showed lower inhibitory potential than analog 34a. The hydroxyl moieties present in the para-position of the phenyl ring showed more interactions with the active site residues. The methoxy moiety instead of the hydroxyl moiety showed fewer interactions with active site residues as well as less activity. Furthermore, the docking studies of compound 34a validated that the oxygen atom of the hydroxyl moiety of the phenyl ring of the compound formed a hydrogen bonding interaction with the active site residues of Lys126 and Glu171 of the α-glucosidase enzyme. The quinoline-Schiff-based derivative is a potential inhibitory agent for the treatment of anti-diabetic activity. Schiff bases of quinoline analogs 34 were synthesized and tested for their anti-diabetic activity against the α-glucosidase enzyme [90]. All the screened molecules exhibited good inhibitory activity with IC50 values ranging from 6.20-48.50 M. Among them, the 3,4-dihydroxy conjugate 34a displayed potent inhibitory activity (IC50 = 6.20 M) against the α-glucosidase enzyme ( Figure 39). The active compound 34a showed a 6-fold greater potency than the reference acarbose (IC50 = 38.45 M). SAR studies revealed that no inhibitory activity was noticed in the presence of electron-donating groups. The position of the hydroxyl moiety plays a role in the activity of these compounds, the relocation of OH groups from 3 and 4-position and their respective presence at the phenyl ring 2,5and 2,3-positions decreased the inhibitory activity and showed lower inhibitory potential than analog 34a. The hydroxyl moieties present in the para-position of the phenyl ring showed more interactions with the active site residues. The methoxy moiety instead of the hydroxyl moiety showed fewer interactions with active site residues as well as less activity. Furthermore, the docking studies of compound 34a validated that the oxygen atom of the hydroxyl moiety of the phenyl ring of the compound formed a hydrogen bonding interaction with the active site residues of Lys126 and Glu171 of the α-glucosidase enzyme. The quinoline-Schiff-based derivative is a potential inhibitory agent for the treatment of anti-diabetic activity. Hybrid molecules 35 having quinoline, pyrazole and thiazole moieties were synthesized and evaluated for their antiproliferative activity [91]. Among them, the hybrid 35a with a para-fluoro substituent on the phenyl ring showed excellent antiproliferative activity (IC50 = 0.136 M) against the cervical (HeLa) cancer cell line (Figure 40). The authors further reported epidermal growth factor receptor (EGFR) inhibitory activity and the active compound showed EGFR inhibitory activity (IC50 Hybrid molecules 35 having quinoline, pyrazole and thiazole moieties were synthesized and evaluated for their antiproliferative activity [91]. Among them, the hybrid 35a with a para-fluoro substituent on the phenyl ring showed excellent antiproliferative activity (IC 50 = 0.136 µM) against the cervical (HeLa) cancer cell line ( Figure 40). The authors further reported epidermal growth factor receptor (EGFR) inhibitory activity and the active compound showed EGFR inhibitory activity (IC 50 = 31.8 nM) at a nanomolar level as compared to the gefitinib standard drug (IC 50 = 29.16 nM). Furthermore, compound 35a displayed H-bonding with Met769, water-mediated H-bonding with Thr766 and a cation-π interaction with Lys721 residues. Compound 35a also showed a hydrophobic interaction by the phenyl moiety with the hydrophobic side-chains of the amino acids Val702, Ala719, Met742 and Leu764 residues of EGFR active site. Therefore, the binding mode of the most active compound at the active site of EGFR confirmed its inhibitory activity, and the potential to act as an anti-proliferative agent through EGFR inhibition. Met742 and Leu764 residues of EGFR active site. Therefore, the binding mode of the most active compound at the active site of EGFR confirmed its inhibitory activity, and the potential to act as an anti-proliferative agent through EGFR inhibition. Another series of quinoline-linked to thiadiazole hybrids 36 were synthesized and the hybrids screened for their antileishmanial potential [92]. All twenty hybrids exhibited good antileishmanial potential with IC50 values ranging from 0.04-9.60 M (Figure 41). Compound 36a with a 2,3dihydroxy group exhibited significant inhibition with an IC50 value of 0.04 M, as compared with the pentamidine standard drug (IC50 = 7.02 M). The superior potential shown by these compounds might be due to the hydroxyl that may be involved in hydrogen bonding. In contrast, halogensubstituted derivatives diminished the antileishmanial potential. Furthermore, compound 36a fitted well in the active site of the pteridine reductase 1 (PTR1) enzyme. The two hydroxyl groups attached to the phenyl ring of the compound forming H-bonds with ArgA17 and AspA181, and the phenyl ring showed a π-interaction with the ArgA17 residue. The -NH of the ArgA17 interacts through its H with the nitrogen of the 1,3,4-thiadiazole moiety of the active compound 36a. The strong bonding network of the compound with the residues of the active pocket might be one of the reasons for its excellent biological activity.

Quinazolines
Quinazoline are nitrogen-containing six-membered heterocyclic compounds that contain a benzene ring system fused to a pyrimidine at two adjacent carbon atoms. Quinazolines and their analogs possess a wide range of biological activities. Many quinazoline compounds were reported as growth factor receptor (EGFR) tyrosine kinase inhibitors, such as gefitinib, erlotinib, lapatinib and afatinib ( Figure 42) [93]. Another series of quinoline-linked to thiadiazole hybrids 36 were synthesized and the hybrids screened for their antileishmanial potential [92]. All twenty hybrids exhibited good antileishmanial potential with IC 50 values ranging from 0.04-9.60 µM (Figure 41). Compound 36a with a 2,3-dihydroxy group exhibited significant inhibition with an IC 50 value of 0.04 µM, as compared with the pentamidine standard drug (IC 50 = 7.02 µM). The superior potential shown by these compounds might be due to the hydroxyl that may be involved in hydrogen bonding. In contrast, halogen-substituted derivatives diminished the antileishmanial potential. Furthermore, compound 36a fitted well in the active site of the pteridine reductase 1 (PTR1) enzyme. The two hydroxyl groups attached to the phenyl ring of the compound forming H-bonds with ArgA17 and AspA181, and the phenyl ring showed a π-interaction with the ArgA17 residue. The-NH of the ArgA17 interacts through its H with the nitrogen of the 1,3,4-thiadiazole moiety of the active compound 36a. The strong bonding network of the compound with the residues of the active pocket might be one of the reasons for its excellent biological activity. Met742 and Leu764 residues of EGFR active site. Therefore, the binding mode of the most active compound at the active site of EGFR confirmed its inhibitory activity, and the potential to act as an anti-proliferative agent through EGFR inhibition. Another series of quinoline-linked to thiadiazole hybrids 36 were synthesized and the hybrids screened for their antileishmanial potential [92]. All twenty hybrids exhibited good antileishmanial potential with IC50 values ranging from 0.04-9.60 M (Figure 41). Compound 36a with a 2,3dihydroxy group exhibited significant inhibition with an IC50 value of 0.04 M, as compared with the pentamidine standard drug (IC50 = 7.02 M). The superior potential shown by these compounds might be due to the hydroxyl that may be involved in hydrogen bonding. In contrast, halogensubstituted derivatives diminished the antileishmanial potential. Furthermore, compound 36a fitted well in the active site of the pteridine reductase 1 (PTR1) enzyme. The two hydroxyl groups attached to the phenyl ring of the compound forming H-bonds with ArgA17 and AspA181, and the phenyl ring showed a π-interaction with the ArgA17 residue. The -NH of the ArgA17 interacts through its H with the nitrogen of the 1,3,4-thiadiazole moiety of the active compound 36a. The strong bonding network of the compound with the residues of the active pocket might be one of the reasons for its excellent biological activity.

Quinazolines
Quinazoline are nitrogen-containing six-membered heterocyclic compounds that contain a benzene ring system fused to a pyrimidine at two adjacent carbon atoms. Quinazolines and their

Quinazolines
Quinazoline are nitrogen-containing six-membered heterocyclic compounds that contain a benzene ring system fused to a pyrimidine at two adjacent carbon atoms. Quinazolines and their analogs possess a wide range of biological activities. Many quinazoline compounds were reported as growth factor receptor (EGFR) tyrosine kinase inhibitors, such as gefitinib, erlotinib, lapatinib and afatinib ( Figure 42) [93]. Quinazoline derivatives 37 were synthesized and evaluated the new derivatives for their antitumor activity [94]. Among them, compound 37a exhibited excellent activity (IC50 = 0.98 M) against the epidermoid carcinoma (A431) cell lines ( Figure 43). A smaller inhibitory activity against all tested tumor cell lines (SW480, A431, A549, NCI-H1975 and HCC827) was observed when the morpholine moiety was replaced with a piperazine moiety. Compound 37a effectively inhibited growth and metastasis in a zebrafish xenograft model. The compound induced cell apoptosis and caused cell cycle arrest at the G0/G1 phase. Furthermore, compound 37a showed increased fluorescence intensity in a dose-dependent manner. Thus, quinazoline derivatives are promising candidates for the development of antitumor agents. A library of 2,4-disubstituted-quinazoline conjugates 38 was synthesized and screened for antitumor activity against five human cancer cell lines, namely, breast (MCF-7 and MDA-MB-231), gastric carcinoma (HGC-27 and MGC-803) and prostate (PC-3) cancer cells by means of the MTT assay [95]. Among them, the trifluoromethyl derivative 38a displayed the most promising anticancer activity (IC50 = 5.10 M) against the MCF-7 breast cancer cell line when compared with the standard gefitinib (IC50 = 7.34 M) (Figure 44). SAR studies revealed that electron-withdrawing groups at the para-position on the phenyl ring showed excellent growth inhibition activity rather than electrondonating groups. Compound 38a caused G1 phase cell cycle arrest and cell apoptosis. Compound 38a markedly decreased p-EGFR and p-PI3K expression, which revealed that compound 38a targeted breast cancer cells via interference with the EGFR-PI3K signaling pathway. Furthermore, compound Quinazoline derivatives 37 were synthesized and evaluated the new derivatives for their antitumor activity [94]. Among them, compound 37a exhibited excellent activity (IC 50 = 0.98 µM) against the epidermoid carcinoma (A431) cell lines ( Figure 43). A smaller inhibitory activity against all tested tumor cell lines (SW480, A431, A549, NCI-H1975 and HCC827) was observed when the morpholine moiety was replaced with a piperazine moiety. Compound 37a effectively inhibited growth and metastasis in a zebrafish xenograft model. The compound induced cell apoptosis and caused cell cycle arrest at the G0/G1 phase. Furthermore, compound 37a showed increased fluorescence intensity in a dose-dependent manner. Thus, quinazoline derivatives are promising candidates for the development of antitumor agents. Quinazoline derivatives 37 were synthesized and evaluated the new derivatives for their antitumor activity [94]. Among them, compound 37a exhibited excellent activity (IC50 = 0.98 M) against the epidermoid carcinoma (A431) cell lines ( Figure 43). A smaller inhibitory activity against all tested tumor cell lines (SW480, A431, A549, NCI-H1975 and HCC827) was observed when the morpholine moiety was replaced with a piperazine moiety. Compound 37a effectively inhibited growth and metastasis in a zebrafish xenograft model. The compound induced cell apoptosis and caused cell cycle arrest at the G0/G1 phase. Furthermore, compound 37a showed increased fluorescence intensity in a dose-dependent manner. Thus, quinazoline derivatives are promising candidates for the development of antitumor agents.  (Figure 44). SAR studies revealed that electron-withdrawing groups at the para-position on the phenyl ring showed excellent growth inhibition activity rather than electron-  [95]. Among them, the trifluoromethyl derivative 38a displayed the most promising anticancer activity (IC 50 = 5.10 µM) against the MCF-7 breast cancer cell line when compared with the standard gefitinib (IC 50 = 7.34 µM) ( Figure 44). SAR studies revealed that electron-withdrawing groups at the para-position on the phenyl ring showed excellent growth inhibition activity rather than electron-donating groups. Compound 38a caused G1 phase cell cycle arrest and cell apoptosis. Compound 38a markedly decreased p-EGFR and p-PI3K expression, which revealed that compound 38a targeted breast cancer cells via interference with the EGFR-PI3K signaling pathway. Furthermore, compound 38a bound well into the active site of EGFR and formed hydrogen bonds with Lys833, Lys890 and Asp836 residues. The benzene ring of aniline interacted with Tyr867 through a π-H interaction, which indicates that compound 38a could tightly connect with EGFR. The disubstituted-quinazoline derivatives are potential molecules for the progress of antitumor agents.
Molecules 2020, 25, x FOR PEER REVIEW 31 of 44 38a bound well into the active site of EGFR and formed hydrogen bonds with Lys833, Lys890 and Asp836 residues. The benzene ring of aniline interacted with Tyr867 through a π-H interaction, which indicates that compound 38a could tightly connect with EGFR. The disubstituted-quinazoline derivatives are potential molecules for the progress of antitumor agents. Another interesting series of quinazoline analogs 39 attached to a benzenesulfonamide was synthesized and evaluated for carbonic anhydrase inhibitory activity against the human CA (hCA) isoforms I, II, IX, and XII [96]. Among them, the unsubstituted phenyl ring compound 39a showed significant inhibitory activity with a Ki value of 0.73 nM against hCA II. Compound 39a was 16-fold more active than the standard acetazolamide (Ki = 12.0 nM) ( Figure 45). SAR studies revealed that phenyl-substituted derivatives are more active than benzyl-substituted derivatives. Unsubstituted quinazoline derivatives showed more significant activity than those derivatives with electrondonating substituents. Quinazoline hybrids 40 with 1,2,3-triazole linkers were prepared and evaluated for their epidermal growth factor receptor (EGFR) tyrosine kinase inhibitory activity [97]. Compound 40a bearing a meta-fluoro benzyl hybrid exhibited potent inhibitory activity (IC50 = 505 nM) against the H1975 cancer cell line ( Figure 46). The compound exhibited 3.5-fold superior inhibitory activity against the EGFR L858R/T790M than the standard afatinib and also displayed 52-and 17-fold selectivity for EGFR L858R/T790M over wild-type HER2 and EGFR. Reduced inhibitory activity was observed in derivatives with electron-donating and electron-neutral group substituents on the benzyl Another interesting series of quinazoline analogs 39 attached to a benzenesulfonamide was synthesized and evaluated for carbonic anhydrase inhibitory activity against the human CA (hCA) isoforms I, II, IX, and XII [96]. Among them, the unsubstituted phenyl ring compound 39a showed significant inhibitory activity with a Ki value of 0.73 nM against hCA II. Compound 39a was 16-fold more active than the standard acetazolamide (Ki = 12.0 nM) ( Figure 45). SAR studies revealed that phenyl-substituted derivatives are more active than benzyl-substituted derivatives. Unsubstituted quinazoline derivatives showed more significant activity than those derivatives with electron-donating substituents.
Molecules 2020, 25, x FOR PEER REVIEW 31 of 44 38a bound well into the active site of EGFR and formed hydrogen bonds with Lys833, Lys890 and Asp836 residues. The benzene ring of aniline interacted with Tyr867 through a π-H interaction, which indicates that compound 38a could tightly connect with EGFR. The disubstituted-quinazoline derivatives are potential molecules for the progress of antitumor agents. Another interesting series of quinazoline analogs 39 attached to a benzenesulfonamide was synthesized and evaluated for carbonic anhydrase inhibitory activity against the human CA (hCA) isoforms I, II, IX, and XII [96]. Among them, the unsubstituted phenyl ring compound 39a showed significant inhibitory activity with a Ki value of 0.73 nM against hCA II. Compound 39a was 16-fold more active than the standard acetazolamide (Ki = 12.0 nM) ( Figure 45). SAR studies revealed that phenyl-substituted derivatives are more active than benzyl-substituted derivatives. Unsubstituted quinazoline derivatives showed more significant activity than those derivatives with electrondonating substituents. Quinazoline hybrids 40 with 1,2,3-triazole linkers were prepared and evaluated for their epidermal growth factor receptor (EGFR) tyrosine kinase inhibitory activity [97]. Compound 40a bearing a meta-fluoro benzyl hybrid exhibited potent inhibitory activity (IC50 = 505 nM) against the H1975 cancer cell line ( Figure 46). The compound exhibited 3.5-fold superior inhibitory activity against the EGFR L858R/T790M than the standard afatinib and also displayed 52-and 17-fold Quinazoline hybrids 40 with 1,2,3-triazole linkers were prepared and evaluated for their epidermal growth factor receptor (EGFR) tyrosine kinase inhibitory activity [97]. Compound 40a bearing a meta-fluoro benzyl hybrid exhibited potent inhibitory activity (IC 50 = 505 nM) against the H1975 cancer cell line ( Figure 46). The compound exhibited 3.5-fold superior inhibitory activity against the EGFR L858R/T790M than the standard afatinib and also displayed 52-and 17-fold selectivity for EGFR L858R/T790M over wild-type HER2 and EGFR. Reduced inhibitory activity was observed in derivatives with electron-donating and electron-neutral group substituents on the benzyl ring than the fluoro-substituted compounds. In addition, compound 40a showed moderate inhibitory activity for the Cyp450 enzyme and less toxicity for hERG and HepG2 cell lines. These results indicate that 40a presents a low risk of cardiac arrhythmia and hepatic toxicity. The quinazoline-triazole hybrids are promising hybrids for the progress of kinase inhibitors for mutant epidermal growth factor receptors.
Molecules 2020, 25, x FOR PEER REVIEW 32 of 44 ring than the fluoro-substituted compounds. In addition, compound 40a showed moderate inhibitory activity for the Cyp450 enzyme and less toxicity for hERG and HepG2 cell lines. These results indicate that 40a presents a low risk of cardiac arrhythmia and hepatic toxicity. The quinazoline-triazole hybrids are promising hybrids for the progress of kinase inhibitors for mutant epidermal growth factor receptors. Quinazoline scaffolds 41 were synthesized and tested for dual EGFR/HER2 tyrosine kinase (TK) inhibitory activity [98]. Among them, compound 41a exhibited potential inhibitory activity (IC50 = 0.31 nM) against the human non-small cell lung cancer cell line HCC 827 when compared with the standard afatinib (IC50 = 0.43 nM) ( Figure 47). In addition, the active compound 41a significantly inhibited in vitro kinase and displayed superior activities with IC50 values of 0.76 nM for EGFR and 39.2 nM for HER2, compared with afatinib (0.96 nM for EGFR and 73.72 nM for HER2). SAR studies revealed that the chiral variation of the alkoxy chain conjugates had poor antiproliferative inhibitory effect on H1975 and HCC827. Furthermore, in in vivo studies, compound 41a showed a better tumor inhibition effects in nude mice NCl-H1975. Thus, the quinazoline derivatives are potential candidates for the development of cancer therapeutic agents.  Quinazoline scaffolds 41 were synthesized and tested for dual EGFR/HER2 tyrosine kinase (TK) inhibitory activity [98]. Among them, compound 41a exhibited potential inhibitory activity (IC 50 = 0.31 nM) against the human non-small cell lung cancer cell line HCC 827 when compared with the standard afatinib (IC 50 = 0.43 nM) ( Figure 47). In addition, the active compound 41a significantly inhibited in vitro kinase and displayed superior activities with IC 50 values of 0.76 nM for EGFR and 39.2 nM for HER2, compared with afatinib (0.96 nM for EGFR and 73.72 nM for HER2). SAR studies revealed that the chiral variation of the alkoxy chain conjugates had poor antiproliferative inhibitory effect on H1975 and HCC827. Furthermore, in in vivo studies, compound 41a showed a better tumor inhibition effects in nude mice NCl-H1975. Thus, the quinazoline derivatives are potential candidates for the development of cancer therapeutic agents.  Quinazoline scaffolds 41 were synthesized and tested for dual EGFR/HER2 tyrosine kinase (TK) inhibitory activity [98]. Among them, compound 41a exhibited potential inhibitory activity (IC50 = 0.31 nM) against the human non-small cell lung cancer cell line HCC 827 when compared with the standard afatinib (IC50 = 0.43 nM) ( Figure 47). In addition, the active compound 41a significantly inhibited in vitro kinase and displayed superior activities with IC50 values of 0.76 nM for EGFR and 39.2 nM for HER2, compared with afatinib (0.96 nM for EGFR and 73.72 nM for HER2). SAR studies revealed that the chiral variation of the alkoxy chain conjugates had poor antiproliferative inhibitory effect on H1975 and HCC827. Furthermore, in in vivo studies, compound 41a showed a better tumor inhibition effects in nude mice NCl-H1975. Thus, the quinazoline derivatives are potential candidates for the development of cancer therapeutic agents.

Pyrimidines
Pyrimidines and pyrimidinones have received considerable attention in organic synthesis because of their wide range of biological activities. The pyrimidine nucleus consists of a six-membered 1,3-diazine ring with a ketone unit. Pyrimidine analogs are an integral part of several biologically active molecules such as natural products and nucleic acids. Moreover, this kind of heterocyclic compound finds several therapeutic applications in medicinal chemistry as an essential building block of a large variety of drug candidates and nucleic acids, with a structural resemblance to purines [99,100]. Recently, the US-FDA approved some pyrimidine and pyrimidinone derivatives (ibrutinib, capecitabine, folinic acid and monastrol) as anticancer agents (Figure 48) [18]. These pyrimidines and their scaffolds exhibit a broad spectrum of bioactivity; hence they occupy privileged positions in drug discovery studies.

Pyrimidines
Pyrimidines and pyrimidinones have received considerable attention in organic synthesis because of their wide range of biological activities. The pyrimidine nucleus consists of a sixmembered 1,3-diazine ring with a ketone unit. Pyrimidine analogs are an integral part of several biologically active molecules such as natural products and nucleic acids. Moreover, this kind of heterocyclic compound finds several therapeutic applications in medicinal chemistry as an essential building block of a large variety of drug candidates and nucleic acids, with a structural resemblance to purines [99,100]. Recently, the US-FDA approved some pyrimidine and pyrimidinone derivatives (ibrutinib, capecitabine, folinic acid and monastrol) as anticancer agents (Figure 48) [18]. These pyrimidines and their scaffolds exhibit a broad spectrum of bioactivity; hence they occupy privileged positions in drug discovery studies. Dihydropyrimidine-5-carboxylic acid analogs 42 were synthesized and evaluated for their xanthine oxidase (XO) inhibitory activity [101]. All the tested molecules exhibited good XO inhibitory activity with IC50 values ranging from 0.018-0567 M. Among them, the iso-butenyl derivative 42a showed excellent inhibitory activity with an IC50 value of 0.018 M, which was almost equal potency as the standard febuxostat (IC50 = 0.023 M) ( Figure 49). Due to amide-enamine tautomerism, the carbonyl group in the position 6 of the pyrimidine ring could function as a hydrogen bonding acceptor or donor and is linked to Thr1010 via a hydrogen bonding. The introduction of a methyl group at the 4-position of the dihydropyrimidine moiety caused a decrease in inhibition potency. Furthermore, compound 42a was evaluated for in vivo hypouricemic effect and significantly reduced the serum uric acid (sUA) and exhibited a promising uric acid lowering property for the treatment of hyperuricemia. The active compound 42a fitted well into the binding pocket of XO and the hydrogen bonding formed between the amino group and Glu802 was stronger than that between the carbonyl group and Thr1010 residue. Therefore, the interaction of the amino group with Glu802 should be emphasized for the design of novel nonpurine XO inhibitors. Dihydropyrimidine-5-carboxylic acid analogs 42 were synthesized and evaluated for their xanthine oxidase (XO) inhibitory activity [101]. All the tested molecules exhibited good XO inhibitory activity with IC 50 values ranging from 0.018-0.567 µM. Among them, the iso-butenyl derivative 42a showed excellent inhibitory activity with an IC 50 value of 0.018 µM, which was almost equal potency as the standard febuxostat (IC 50 = 0.023 µM) (Figure 49). Due to amide-enamine tautomerism, the carbonyl group in the position 6 of the pyrimidine ring could function as a hydrogen bonding acceptor or donor and is linked to Thr1010 via a hydrogen bonding. The introduction of a methyl group at the 4-position of the dihydropyrimidine moiety caused a decrease in inhibition potency. Furthermore, compound 42a was evaluated for in vivo hypouricemic effect and significantly reduced the serum uric acid (sUA) and exhibited a promising uric acid lowering property for the treatment of hyperuricemia. The active compound 42a fitted well into the binding pocket of XO and the hydrogen bonding formed between the amino group and Glu802 was stronger than that between the carbonyl group and Thr1010 residue. Therefore, the interaction of the amino group with Glu802 should be emphasized for the design of novel nonpurine XO inhibitors. Dihydropyrimidinone conjugates 43 were created and tested for antiproliferative and tubulin polymerization inhibitory activities [102]. Among the tested compounds, the derivative 43a with a 4methylphenyl ring exhibited the highest antiproliferation activity with IC50 values of 0.54 M and 1.18 M against the MCF-7 and MDA-MB-231 breast cancer cell lines ( Figure 50). The compounds bearing five-membered heterocyclic thiophenyl and furanyl rings at the C4 position showed moderate cytotoxicity in comparison with derivatives with a methyl substitutent on the phenyl ring. Compound 43a caused cell cycle arrest at the G2/M phase in a dose-dependent manner and effectively inhibited tubulin polymerization. Furthermore, compound 43a was evaluated by molecular docking studies, and which was formed three hydrogen bonding interactions with the catalytic active site (CAS) residues Ser178, Val238 and Val318 of α/β-tubulin.  Dihydropyrimidinone conjugates 43 were created and tested for antiproliferative and tubulin polymerization inhibitory activities [102]. Among the tested compounds, the derivative 43a with a 4-methylphenyl ring exhibited the highest antiproliferation activity with IC 50 values of 0.54 µM and 1.18 µM against the MCF-7 and MDA-MB-231 breast cancer cell lines ( Figure 50). The compounds bearing five-membered heterocyclic thiophenyl and furanyl rings at the C4 position showed moderate cytotoxicity in comparison with derivatives with a methyl substitutent on the phenyl ring. Compound 43a caused cell cycle arrest at the G2/M phase in a dose-dependent manner and effectively inhibited tubulin polymerization. Furthermore, compound 43a was evaluated by molecular docking studies, and which was formed three hydrogen bonding interactions with the catalytic active site (CAS) residues Ser178, Val238 and Val318 of α/ β-tubulin. Dihydropyrimidinone conjugates 43 were created and tested for antiproliferative and tubulin polymerization inhibitory activities [102]. Among the tested compounds, the derivative 43a with a 4methylphenyl ring exhibited the highest antiproliferation activity with IC50 values of 0.54 M and 1.18 M against the MCF-7 and MDA-MB-231 breast cancer cell lines ( Figure 50). The compounds bearing five-membered heterocyclic thiophenyl and furanyl rings at the C4 position showed moderate cytotoxicity in comparison with derivatives with a methyl substitutent on the phenyl ring. Compound 43a caused cell cycle arrest at the G2/M phase in a dose-dependent manner and effectively inhibited tubulin polymerization. Furthermore, compound 43a was evaluated by molecular docking studies, and which was formed three hydrogen bonding interactions with the catalytic active site (CAS) residues Ser178, Val238 and Val318 of α/β-tubulin.   Figure 51). Compound 44a also strongly inhibited the FAK enzyme (IC 50 = 28.2 µM) and induced apoptosis in a dose-dependent manner of MDA-MB-231. Compound 44a caused cell cycle arrest at the G0/G1 phase and showed low cytotoxicity against the normal human cell line (HK2). Furthermore, compound 44a can be docked into the ATP-binding site of FAK where it was anchored to the hinge region via canonical donor-acceptor hydrogen-bonding motifs between the nitrogen molecules on the 2,7-disubstituted thieno [3,2-  Pyrimidine scaffolds 45 were synthesized and evaluated the scaffolds for Janus kinase 3 (JAK3) inhibitory activity [104]. Among them compound 45a with a morpholine unit attached to the phenyl ring displayed the highest JAK3 inhibitory activity with an IC50 value of 1.7 M, compared with the standard tofacitinib (IC50 = 0.9 M) ( Figure 52). The 4-morpholinoaniline exhibited a higher potency than a pyrazole ring at the 2-position of the pyrimidine ring. Compound 45a strongly inhibited T cell proliferation (IC50 = 0.83 M) and high selectivity within the JAK family. Moreover, in vivo data revealed that compound 45a significantly suppressed oxazolone (OXZ)-induced delayed hypersensitivity responses in Balb/c mice. The compound showed good oral pharmacokinetic properties. Furthermore, the molecular docking study of compound 45a, revealed that the hydrophobic aromatic amine group showed σ-π interactions with the amino acid Leu828 and Gly908 residues, and the bidentate hinge hydrogen bonding formed with the Leu905 residue in the active sites of JAK3. Therefore, the compound is a potential candidate as a selective JAK3 inhibitor for treating autoimmune diseases.  Pyrimidine scaffolds 45 were synthesized and evaluated the scaffolds for Janus kinase 3 (JAK3) inhibitory activity [104]. Among them compound 45a with a morpholine unit attached to the phenyl ring displayed the highest JAK3 inhibitory activity with an IC 50 value of 1.7 µM, compared with the standard tofacitinib (IC 50 = 0.9 µM) ( Figure 52). The 4-morpholinoaniline exhibited a higher potency than a pyrazole ring at the 2-position of the pyrimidine ring. Compound 45a strongly inhibited T cell proliferation (IC 50 = 0.83 µM) and high selectivity within the JAK family. Moreover, in vivo data revealed that compound 45a significantly suppressed oxazolone (OXZ)-induced delayed hyper-sensitivity responses in Balb/c mice. The compound showed good oral pharmacokinetic properties. Furthermore, the molecular docking study of compound 45a, revealed that the hydrophobic aromatic amine group showed σ-π interactions with the amino acid Leu828 and Gly908 residues, and the bidentate hinge hydrogen bonding formed with the Leu905 residue in the active sites of JAK3. Therefore, the compound is a potential candidate as a selective JAK3 inhibitor for treating autoimmune diseases.  Pyrimidine scaffolds 45 were synthesized and evaluated the scaffolds for Janus kinase 3 (JAK3) inhibitory activity [104]. Among them compound 45a with a morpholine unit attached to the phenyl ring displayed the highest JAK3 inhibitory activity with an IC50 value of 1.7 M, compared with the standard tofacitinib (IC50 = 0.9 M) ( Figure 52). The 4-morpholinoaniline exhibited a higher potency than a pyrazole ring at the 2-position of the pyrimidine ring. Compound 45a strongly inhibited T cell proliferation (IC50 = 0.83 M) and high selectivity within the JAK family. Moreover, in vivo data revealed that compound 45a significantly suppressed oxazolone (OXZ)-induced delayed hypersensitivity responses in Balb/c mice. The compound showed good oral pharmacokinetic properties. Furthermore, the molecular docking study of compound 45a, revealed that the hydrophobic aromatic amine group showed σ-π interactions with the amino acid Leu828 and Gly908 residues, and the bidentate hinge hydrogen bonding formed with the Leu905 residue in the active sites of JAK3. Therefore, the compound is a potential candidate as a selective JAK3 inhibitor for treating autoimmune diseases.   Figure 53). The compound 46a also exhibited 3-fold stronger CDK2 inhibitory potency (IC 50 = 15.4 nM) than the standard AZD5438 (IC 50 = 45 nM). Replacement of the methyl group with a fluoro atom in the pyrimidine hybrid resulted in reduced potency against both HeLa and HCT116 cells. Compound 46a effectively induced apoptosis and cell cycle arrest at the G2/M phase in a concentration-dependent manner. Furthermore, the active compound 46a exhibited effective binding into the CDK2 binding site. The oxygen and nitrogen atoms of the sulfonyl moiety interacted with the Asp86 residue through hydrogen bonding. Moreover, the Leu83 residue formed hydrogen bonding with the 2-amino moiety and nitrogen on the pyrimidine ring. Another important hydrogen bonding was detected between the sulfur atom on the benzothiazole ring and the residue Lys33. These pyrimidine hybrids are potential analogs as a cyclin-dependent kinase 2 (CDK2) inhibitors.  Pyrimidine fused pyrazole hybrids 47 were synthesized and tested for their anti-tubercular activity against the H37Rv strain [106]. All the tested hybrids showed good to excellent antitubercular activity with MIC values ranging from 0.8 to 50 g/mL ( Figure 54). Among them, the fluoro-substituted hybrid 47a exhibited the most potent anti-tubercular activity, with a MIC value of 0.8 g/mL, which was almost 4-fold stronger potency than the standard ciprofloxacin (MIC = 3.12 g/mL). SAR studies revealed that electron-donating groups showed reduced the activity in comparison with halogen-substituted derivatives. Furthermore, the active compound 47a displayed no cytotoxicity and the H-bonding interactions with Lys165, Tyr196, and Ile194 through the water molecule, contributed to the binding affinity of the ligand. These compounds are promising hybrids for the development of novel anti-tubercular agents.  Pyrimidine fused pyrazole hybrids 47 were synthesized and tested for their anti-tubercular activity against the H37Rv strain [106]. All the tested hybrids showed good to excellent anti-tubercular activity with MIC values ranging from 0.8 to 50 µg/mL ( Figure 54). Among them, the fluoro-substituted hybrid 47a exhibited the most potent anti-tubercular activity, with a MIC value of 0.8 µg/mL, which was almost 4-fold stronger potency than the standard ciprofloxacin (MIC = 3.12 µg/mL). SAR studies revealed that electron-donating groups showed reduced the activity in comparison with halogen-substituted derivatives. Furthermore, the active compound 47a displayed no cytotoxicity and the H-bonding interactions with Lys165, Tyr196, and Ile194 through the water molecule, contributed to the binding affinity of the ligand. These compounds are promising hybrids for the development of novel anti-tubercular agents.  Pyrimidine fused pyrazole hybrids 47 were synthesized and tested for their anti-tubercular activity against the H37Rv strain [106]. All the tested hybrids showed good to excellent antitubercular activity with MIC values ranging from 0.8 to 50 g/mL ( Figure 54). Among them, the fluoro-substituted hybrid 47a exhibited the most potent anti-tubercular activity, with a MIC value of 0.8 g/mL, which was almost 4-fold stronger potency than the standard ciprofloxacin (MIC = 3.12 g/mL). SAR studies revealed that electron-donating groups showed reduced the activity in comparison with halogen-substituted derivatives. Furthermore, the active compound 47a displayed no cytotoxicity and the H-bonding interactions with Lys165, Tyr196, and Ile194 through the water molecule, contributed to the binding affinity of the ligand. These compounds are promising hybrids for the development of novel anti-tubercular agents.  Pyrimidine hybrids 48 with a 1,2,4-triazole linker were created and tested for antiproliferative activity against four human alveolar epithelial (A549), cervical (HeLa), colon (HCT116) and embryonic kidney (HEK293) cancer cells [107]. Compound 48a bearing a para-nitro group on the phenyl ring exhibited the most significant antiproliferative activity against A549 and HeLa cells with IC 50 values of 1.02 µM and 0.75 µM, respectively ( Figure 55). Electron-withdrawing groups, such as 4-nitro, 3,5-dibromo, 3,4-dichloro, 4-bromo, and 3-fluoro on the phenyl ring hybrids showed more activity than compounds with electron-donating groups (2,4-dimethoxy, 4-dimethoxy, 3,4,5-trimethoxy, 4-ethyl, and 3,4-dimethoxy). Compound 48a caused cell cycle arrest at the G2/M phase. Furthermore, molecular docking studies of the active compound 48a showed that it occupied the colchicine-binding site of tubulin. The oxygen of the 4-nitrophenyl ring established a hydrogen bonding interaction with βCys241 and one nitrogen atom of the triazole ring forms a hydrogen bonding with βAsn258, and the residue of αThr178 forms a hydrogen bonding with the oxygen of the trimethoxy group. These hybrids are potential candidates for the development of anticancer agents. Pyrimidine hybrids 48 with a 1,2,4-triazole linker were created and tested for antiproliferative activity against four human alveolar epithelial (A549), cervical (HeLa), colon (HCT116) and embryonic kidney (HEK293) cancer cells [107]. Compound 48a bearing a para-nitro group on the phenyl ring exhibited the most significant antiproliferative activity against A549 and HeLa cells with IC50 values of 1.02 M and 0.75 M, respectively ( Figure 55). Electron-withdrawing groups, such as 4-nitro, 3,5-dibromo, 3,4-dichloro, 4-bromo, and 3-fluoro on the phenyl ring hybrids showed more activity than compounds with electron-donating groups (2,4-dimethoxy, 4-dimethoxy, 3,4,5trimethoxy, 4-ethyl, and 3,4-dimethoxy). Compound 48a caused cell cycle arrest at the G2/M phase. Furthermore, molecular docking studies of the active compound 48a showed that it occupied the colchicine-binding site of tubulin. The oxygen of the 4-nitrophenyl ring established a hydrogen bonding interaction with βCys241 and one nitrogen atom of the triazole ring forms a hydrogen bonding with βAsn258, and the residue of αThr178 forms a hydrogen bonding with the oxygen of the trimethoxy group. These hybrids are potential candidates for the development of anticancer agents. Pyrimidine-linked nitroxide derivatives 49 were synthesized and screened for anti-proliferative and Aurora kinases inhibitory activities [108]. Among the series, the butyl-and fluoro-substituted compound 49a exhibited the most significant activity, with an IC50 value of 0.89 M against the lung carcinoma (A-549) cancer cell line ( Figure 56). Compound 49a also exhibited kinase inhibitory activity against Aurora-A (IC50 = 9.3 M) and Aurora-B (IC50 = 2.8 M) in HeLa cells. In addition, compound 49a effectively inhibited the phosphorylation of HisH3 and reduced the expression of protein Eg5 and TPX2 in a dose-dependent manner. Furthermore, compound 49a bound well with Aurora-A and Aurora-B. For Aurora-A, the pyrimidine ring of 49a occupied the adenine-binding region and formed an essential hydrogen-bonding interaction with Ala213 in the hinge region. For Aurora-B, the two nitrogen atoms of the pyrazole group in 49a formed hydrogen-bonding interactions with the key amino acid residues Glu155 and Ala157 of the hinge region and the amide bond at C-2 of the pyrimidine form hydrogen-bonding interaction with Pro158 residues. The pyrimidine-linked nitroxide analogs are potent molecules for the development of anticancer agents as Aurora kinase inhibitors. Pyrimidine-linked nitroxide derivatives 49 were synthesized and screened for anti-proliferative and Aurora kinases inhibitory activities [108]. Among the series, the butyl-and fluoro-substituted compound 49a exhibited the most significant activity, with an IC 50 value of 0.89 µM against the lung carcinoma (A-549) cancer cell line ( Figure 56). Compound 49a also exhibited kinase inhibitory activity against Aurora-A (IC 50 = 9.3 µM) and Aurora-B (IC 50 = 2.8 µM) in HeLa cells. In addition, compound 49a effectively inhibited the phosphorylation of HisH3 and reduced the expression of protein Eg5 and TPX2 in a dose-dependent manner. Furthermore, compound 49a bound well with Aurora-A and Aurora-B. For Aurora-A, the pyrimidine ring of 49a occupied the adenine-binding region and formed an essential hydrogen-bonding interaction with Ala213 in the hinge region. For Aurora-B, the two nitrogen atoms of the pyrazole group in 49a formed hydrogen-bonding interactions with the key amino acid residues Glu155 and Ala157 of the hinge region and the amide bond at C-2 of the pyrimidine form hydrogen-bonding interaction with Pro158 residues. The pyrimidine-linked nitroxide analogs are potent molecules for the development of anticancer agents as Aurora kinase inhibitors. Molecules 2020, 25, x FOR PEER REVIEW 38 of 44 Figure 56. Antiproliferative activity of the most potent pyrimidine-linked nitroxide derivative 49a.

Conclusions
The scope of nitrogen-based compounds in medicine is growing daily and their diverse analogs provide a viable and important path for the discover of drugs with various biological applications. The N-heterocyclic frameworks offer a high degree of structural diversity that has proven useful for the search of new therapeutic agents in improving the pharmacokinetics and other physicochemical features. Numerous drugs that are currently in clinical practice have fatal side-effects and have developed multidrug resistance, and have been extensively used in practice to treat various types of diseases with high therapeutic potency. Research and development of nitrogen-based compounds in medicinal chemistry has become a rapidly developing and increasingly active topic. A large amount of work has been made towards N-heterocyclic skeleton medicinal chemistry. The overwhelming advantages of nitrogen-containing drugs in the medicinal field, including easy preparation, low toxicity, less adverse effects, high bioavailability, lower drug resistance, good biocompatibility, etc., encourage efforts towards further research and development. Hence, the properties of these scaffolds are vital to the synthetic strategy in the current drug discovery and design system. In this review, we mainly covered and widely described the current trends in families of nitrogen-based heterocyclic molecules namely, β-lactam, pyrazole, imidazole, 1,2,4-triazole, pyrimidine, quinoline and quinazoline derivatives, with highly promising biological properties such as anticancer, antiinflammatory, antibacterial, antifungal, antitubercular, antidiabetic, antioxidant, anti-HIV and other medicinal properties. In addition, we have explored their structure-activity relationships, as well as a binding mode through molecular docking studies. The SAR studies of the discussed molecules offered a greater understanding of the pattern of substituents on their basic skeleton and appropriate substitutions accountable for its effectiveness. These significant points confirm the enormous potential of various N-heterocyclic cores in pharmaceutical applications suggesting a massive scope for these promising moieties because of their diverse molecular targets. We believe that this review article will be valuable for encouraging the structural design and development of sustainable and effective nitrogen-based drugs against various diseases, with minimal side-effects.

Conclusions
The scope of nitrogen-based compounds in medicine is growing daily and their diverse analogs provide a viable and important path for the discover of drugs with various biological applications. The N-heterocyclic frameworks offer a high degree of structural diversity that has proven useful for the search of new therapeutic agents in improving the pharmacokinetics and other physicochemical features. Numerous drugs that are currently in clinical practice have fatal side-effects and have developed multidrug resistance, and have been extensively used in practice to treat various types of diseases with high therapeutic potency. Research and development of nitrogen-based compounds in medicinal chemistry has become a rapidly developing and increasingly active topic. A large amount of work has been made towards N-heterocyclic skeleton medicinal chemistry. The overwhelming advantages of nitrogen-containing drugs in the medicinal field, including easy preparation, low toxicity, less adverse effects, high bioavailability, lower drug resistance, good biocompatibility, etc., encourage efforts towards further research and development. Hence, the properties of these scaffolds are vital to the synthetic strategy in the current drug discovery and design system. In this review, we mainly covered and widely described the current trends in families of nitrogen-based heterocyclic molecules namely, β-lactam, pyrazole, imidazole, 1,2,4-triazole, pyrimidine, quinoline and quinazoline derivatives, with highly promising biological properties such as anticancer, anti-inflammatory, antibacterial, antifungal, antitubercular, antidiabetic, antioxidant, anti-HIV and other medicinal properties. In addition, we have explored their structure-activity relationships, as well as a binding mode through molecular docking studies. The SAR studies of the discussed molecules offered a greater understanding of the pattern of substituents on their basic skeleton and appropriate substitutions accountable for its effectiveness. These significant points confirm the enormous potential of various N-heterocyclic cores in pharmaceutical applications suggesting a massive scope for these promising moieties because of their diverse molecular targets. We believe that this review article will be valuable for encouraging the structural design and development of sustainable and effective nitrogen-based drugs against various diseases, with minimal side-effects.

Author Contributions:
The authors (N.K., L.G., S.M., K.K.G. and S.B.J.) have equally contributed to this work. All authors have read and agreed to the published version of the manuscript.

Funding:
The research received no external funding.