Structural Elucidation of Malonylcommunol and 6β-Hydroxy-trans-communic Acid, Two Undescribed Diterpenes from Salvia cinnabarina. First Examples of Labdane Diterpenoids from a Mexican Salvia Species.

The aerial parts of Salvia cinnabarina afforded two undescribed labdane diterpenoids 1 and 2 (malonylcommunol and 6β-hydroxy-trans-communic acid) along with two known labdane diterpenoids, trans-communic acid (3) and trans-communol (4). Additionally, seven known metabolites were also isolated; two isopimarane diterpenoids 5 and 6, two sesquiterpenoids identified as β-eudesmol (7) and cryptomeridiol (8), and three aromatic compounds identified as phthalic acid (9), a mixture of tyrosol fatty acid esters (10) and the flavone salvigenine (11). While compounds compounds 1–3 showed significant inhibition of yeast α-glucosidase, compounds 2, 3 and 7 had no anti-inflammatory activity in the edema model induced by TPA. This paper is not only the first report on a wild population of Salvia cinnabarina, but also of the presence of labdane-type diterpenoids in a Mexican Salvia sp.


Introduction
Salvia L. is the largest genus of Lamiaceae family of plants, with over 1000 species distributed worldwide [1]. The name of the genus derives from the Latin verb "salvare" which means to heal. This is very likely due to the fact that some of these plant species (Salvia divinorum Epling and Játiva, S. milthiorrhiza Bunge and S. officinalis L. [2]) have been used since ancient times to treat several ailments, and are important medicinal herbs in both traditional and modern medicine. Mexico is one of the most important areas of diversification of the genus in the world, with over 319 species, representing ca. 32% of the total, although this number is continuously increasing due to the discovery of new species [3]. Several Salvia species are utilized in different regions of the country for treatment of various ailments, with some well recognized as part of several medicinal plant complexes [2].
Salvia cinnabarina M. Martens and Galeotti (Section Incarnatae), a plant originating from Mexico, is used for the treatment of colic and rheumatism in the Mexican state of Chiapas. Antibacterial and spasmolytic activities have been also described for this species [4].
Previous studies on S. cinnabarina include the analysis of volatile organic compounds [5], and the essential oil obtained from fresh aerial parts of the plant by steam distillation [6], as well as phytochemical analysis of the leaf exudate leading to the isolation of a 3,4-seco-isopimarane diterpenoid whose structure and relative stereochemistry was established as 3,4-seco-isopimara-4(18),7,15-trien-3-oic acid (12) by diterpenoid whose structure and relative stereochemistry was established as 3,4-seco-isopimara-4(18),7,15-trien-3-oic acid (12) by spectroscopic and X-ray diffraction analysis [7,8]. Compound 12 has been tested in several in vitro and in vivo models and exhibits a wide array of biological activities, such as antispasmodic in the isolated guinea-pig ileum model, inhibition of urinary bladder contractility in rats [9] and intestinal motility in mice [10]. Hypotensive activity in a rat model [11], along with anxiolytic and anti-depressive effects in the elevated plus-maze and the forced swimming tests in mice have also been described [12]. Compound 12 showed antimutagenic activity in the Ames test on Salmonella typhimurium and Escherichia coli [13] and also had an anticlastogenic effect in human lymphocytes of its sodium salt [14]. It should be noted that, up to now, all the studies on S. cinnabarina, have been carried out solely on cultivated material from different Botanical Gardens.

Results and Discussion
The aerial parts of Salvia cinnabarina afforded 11 compounds (Figure 1) after extensive chromatographic separation and purification.  Compound 1 was isolated as a solid, m.p. = 85-90 • C. The mass spectrum obtained by DART technique allowed to establish the chemical formula as C 23 H 34 O 4 with seven degrees of unsaturation. The 13 C NMR spectrum (Table 1) of 1, corroborated the presence of 23 carbon atoms, which, according to the HSQC spectrum correspond to ten methylenes (two sp 2 and eight sp 3 ), four methines (two sp 2 and two sp 3 ), two quaternary carbons, four nonprotonated carbons and three methyl groups. In the 13 C NMR spectrum of 1 (Table 1), signals for an exocyclic methylene, such as the one present at C-8:C-17 of trans-communic acid (3) [16] and trans-communol (4) [16] are observed at δ 147.7 (C) and 108.2 (CH 2 ) ppm. Observed signals for carbon atoms of a terminal vinyl group at δ 141.7 (CH) and 110.1 (CH 2 ), together with those observed at 133.8 (CH), 133.7 (C), 23.3 (CH 2 ) and 12.0 (CH 3 ), suggested that compound 1 has a side chain identical to the one present in diterpenes 3 and 4. Therefore, the signals at δ 141.7 and 110.1 were assigned to C-14 and C-15 and those at 133.8, 133.7, 23.3 and 12.0 ppm, to C-12, C-13, C-11 and C-16, respectively. The chemical shifts of C-14 (141.7) and C-16 (12.0) confirmed the configuration of the C-12:C-13 double bond as E (trans). The chemical shifts of these carbon atoms are very sensitive to the double bond configuration, being observed at approximately 130 and 20 ppm in the case of a Z (cis) configuration [17,18]. Characteristic signals for the hydrogen atoms of the exocyclic methylene at C-8 (H-17) were also observed in the 1 H NMR spectrum of 1 as broad singlets at 4.82 and 4.47 ppm.
A relevant signal in the 1 H NMR spectrum for the structural assignment of diterpene 1, was a broad singlet integrating for two hydrogen atoms at 3.41 ppm, that disappears upon addition of D 2 O. This signal correlated in the HMBC spectrum (Table 1) with two carbonyl signals located at 169.6, 168.3 and a methylene signal at 68.5 ppm. The first two signals are assigned to the carbonyls of an acid and an ester, respectively, and the third to a methylene whose hydrogen atoms are observed in the 1 H NMR spectrum, as an AB system at 4.38 and 3.95 ppm (J = 10.9). The IR spectrum of 1 is congruent with the existence of a carboxylic acid and an ester group in this compound, since a broad band centered at approximately 3000 cm −1 a carbonyl band in 1721 cm −1 (characteristic of a carboxylic acid) and a carbonyl ester band at 1736 cm −1 were observed. The above discussion, and the similarity between the NMR spectra of trans-communol (4) and those of 1, allows us to conclude the presence of a malonic acid ester at position C-19 in compound 1, which was named malonylcommunol (1). The exchange of the protons of the methylene group at 3.41 ppm of the malonyl group upon addition of D 2 O could be explained by the enolization of the 1,3 dicarbonyl moiety [19]. The NOESY spectrum of 1 confirms the structure and relative stereochemistry proposed for this unpublished diterpene isolated from S. cinnabarina, on account of observation of expected interactions, illustrated in Figure 2. A malonate ester of a labdane diterpenoid from Calceolaria corymbosa Ruiz and Pav (Scrophulariaceae), with the same connectivity as malonylcommunol (1), was isolated in 1993 by Garbarino and Molinari [17]. However, the double bond has a Z configuration between carbons C-12 and C-13 in the diterpene from C. corymbosa. According to the authors it belongs to the ent-labdane series and therefore is a stereoisomer of 1. To establish the absolute configuration of 1, its experimental ECD spectrum (Figure 3) was recorded and compared with those registered for compounds 3 and 4 ( Figure 3), whose stereochemistry has been previously determined [16]. The ECD spectrum of 1 displayed a negative Cotton effect at 203 nm and a positive one at 226 nm and was in good agreement with those of 3 and 4. Additionally, ECD calculations for the 4S5R9S10R diastereomers of 3 and 4 and their enantiomers (4R5S9R10S) were performed, interestingly, the curves matched the calculated for diasteroisomers 4S5R9S10R ( Figure 3). Thus, the absolute configuration of compound 1 was determined to be 4S5R9S10R. A relevant signal in the 1 H NMR spectrum for the structural assignment of diterpene 1, was a broad singlet integrating for two hydrogen atoms at 3.41 ppm, that disappears upon addition of D2O. This signal correlated in the HMBC spectrum (Table 1) with two carbonyl signals located at 169.6, 168.3 and a methylene signal at 68.5 ppm. The first two signals are assigned to the carbonyls of an acid and an ester, respectively, and the third to a methylene whose hydrogen atoms are observed in the 1 H NMR spectrum, as an AB system at 4.38 and 3.95 ppm (J = 10.9). The IR spectrum of 1 is congruent with the existence of a carboxylic acid and an ester group in this compound, since a broad band centered at approximately 3000 cm −1 a carbonyl band in 1721 cm −1 (characteristic of a carboxylic acid) and a carbonyl ester band at 1736 cm −1 were observed. The above discussion, and the similarity between the NMR spectra of trans-communol (4) and those of 1, allows us to conclude the presence of a malonic acid ester at position C-19 in compound 1, which was named malonylcommunol (1). The exchange of the protons of the methylene group at 3.41 ppm of the malonyl group upon addition of D2O could be explained by the enolization of the 1,3 dicarbonyl moiety [19]. The NOESY spectrum of 1 confirms the structure and relative stereochemistry proposed for this unpublished diterpene isolated from S. cinnabarina, on account of observation of expected interactions, illustrated in Figure  2. A malonate ester of a labdane diterpenoid from Calceolaria corymbosa Ruiz and Pav (Scrophulariaceae), with the same connectivity as malonylcommunol (1), was isolated in 1993 by Garbarino and Molinari [17]. However, the double bond has a Z configuration between carbons C-12 and C-13 in the diterpene from C. corymbosa. According to the authors it belongs to the ent-labdane series and therefore is a stereoisomer of 1. To establish the absolute configuration of 1, its experimental ECD spectrum (Figure 3) was recorded and compared with those registered for compounds 3 and 4 ( Figure 3), whose stereochemistry has been previously determined [16]. The ECD spectrum of 1 displayed a negative Cotton effect at 203 nm and a positive one at 226 nm and was in good agreement with those of 3 and 4. Additionally, ECD calculations for the 4S5R9S10R diastereomers of 3 and 4 and their enantiomers (4R5S9R10S) were performed, interestingly, the curves matched the calculated for diasteroisomers 4S5R9S10R ( Figure 3). Thus, the absolute configuration of compound 1 was determined to be 4S5R9S10R.   C a lc u la te d 3 C a lc u la te d 4 C a lc u la te d e n t-3 C a lc u la te d e n t-4 Diterpene malonates are relatively common, several examples and even products in which both acidic functions of malonic acid are esterified with diterpenic alcohols have been described [20][21][22][23][24][25][26][27]. Compound 1 is an unpublished malonate diterpenoid and the first described in any Salvia species.
The second unreported labdane diterpenoid obtained from the dichloromethane extract of S. cinnabarina was isolated as a white solid, m.p. 170-173 °C, whose molecular formula established by mass spectrometry as C20H30O3. These data, in addition to the 1 H and 13 C NMR data (Table 2), allow us to propose structure 2 for this undescribed diterpene. The 1 H NMR spectrum exhibits similar signals to those observed for trans-communic acid (3). The main difference in the spectrum of 2 is the presence of a wide quartet-like signal centered at 4.52 (J = 2.1 Hz) ppm that is assigned to a hydrogen atom geminal to a hydroxyl group which interacts with a broad singlet at 1.46 ppm and with the signals of a methylene at 2.34 and 2.50 ppm according to the correlations observed in its COSY spectrum. The signal at 1.46 ppm correlates in the HSQC spectrum (Table 2) with a sp 3 methine carbon observed at 57.5 ppm in the 13 C NMR spectrum of 2 which is assigned by its chemical shift to C-5. These facts allow locating the hydroxyl group at C-6 position and the coupling constants of the observed signal for its geminal hydrogen atom (H-6) indicate a β-axial orientation for the OH group. The analysis of the NOESY spectrum confirms the structure and relative stereochemistry assigned to this product, as the expected correlations are observed and the most relevant being those of H-5 with H-6 and the C-18 methyl ( Figure 4). In its IR spectrum characteristic bands for hydroxy groups in 3684, 3590 and 3531 cm −1 were observed, as well as a broad band centered at approximately 3000 cm −1 attributed to the hydroxy group of a carboxylic acid, whose carbonyl group is observed at 1725 cm −1 . In the spectrum, signals attributable to double bonds at 1647 and 1605 cm −1 were also observed. Based on the previous discussion, product 2 should be named 6β-hydroxy-trans-communic acid, which has not been previously described. The absolute configuration of 6β-hydroxy-trans-communic acid (2) was established to be 4S5R6R9S10R by comparison of its experimental ECD curve with those recorded for 1, 3 and 4, which coexist in this population of S. cinnabarina. In addition, in 1965, a diterpene called zanzibaric acid was isolated from Trachylobium verrucosum Engl., whose structure and absolute configuration were established by spectroscopic means, as well as chemical correlation with a derivative of neo-abietic acid, establishing that zanzibaric acid is an ent-labdane [28]. Treatment of the zanzibaric acid methyl ester with NaOH in ethanol gave a product called 6deacetylzanzibaric acid, whose connectivity is similar to that found for product 2. However, comparison of m.p. and the specific rotation indicates that they are diastereoisomeric substances, as indicated in Figure 5. Diterpene malonates are relatively common, several examples and even products in which both acidic functions of malonic acid are esterified with diterpenic alcohols have been described [20][21][22][23][24][25][26][27]. Compound 1 is an unpublished malonate diterpenoid and the first described in any Salvia species.
The second unreported labdane diterpenoid obtained from the dichloromethane extract of S. cinnabarina was isolated as a white solid, m.p. 170-173 • C, whose molecular formula established by mass spectrometry as C 20 H 30 O 3 . These data, in addition to the 1 H and 13 C NMR data (Table 2), allow us to propose structure 2 for this undescribed diterpene. The 1 H NMR spectrum exhibits similar signals to those observed for trans-communic acid (3). The main difference in the spectrum of 2 is the presence of a wide quartet-like signal centered at 4.52 (J = 2.1 Hz) ppm that is assigned to a hydrogen atom geminal to a hydroxyl group which interacts with a broad singlet at 1.46 ppm and with the signals of a methylene at 2.34 and 2.50 ppm according to the correlations observed in its COSY spectrum. The signal at 1.46 ppm correlates in the HSQC spectrum (Table 2) with a sp 3 methine carbon observed at 57.5 ppm in the 13 C NMR spectrum of 2 which is assigned by its chemical shift to C-5. These facts allow locating the hydroxyl group at C-6 position and the coupling constants of the observed signal for its geminal hydrogen atom (H-6) indicate a β-axial orientation for the OH group. The analysis of the NOESY spectrum confirms the structure and relative stereochemistry assigned to this product, as the expected correlations are observed and the most relevant being those of H-5 with H-6 and the C-18 methyl ( Figure 4). In its IR spectrum characteristic bands for hydroxy groups in 3684, 3590 and 3531 cm −1 were observed, as well as a broad band centered at approximately 3000 cm −1 attributed to the hydroxy group of a carboxylic acid, whose carbonyl group is observed at 1725 cm −1 . In the spectrum, signals attributable to double bonds at 1647 and 1605 cm −1 were also observed. Based on the previous discussion, product 2 should be named 6β-hydroxy-trans-communic acid, which has not been previously described. The absolute configuration of 6β-hydroxy-trans-communic acid (2) was established to be 4S5R6R9S10R by comparison of its experimental ECD curve with those recorded for 1, 3 and 4, which coexist in this population of S. cinnabarina. In addition, in 1965, a diterpene called zanzibaric acid was isolated from Trachylobium verrucosum Engl., whose structure and absolute configuration were established by spectroscopic means, as well as chemical correlation with a derivative of neo-abietic acid, establishing that zanzibaric acid is an ent-labdane [28]. Treatment of the zanzibaric acid methyl ester with NaOH in ethanol gave a product called 6-deacetylzanzibaric acid, whose connectivity is similar to that found for product 2. However, comparison of m.p. and the specific rotation indicates that they are diastereoisomeric substances, as indicated in Figure 5.    Compound 3, isolated from this population of S. cinnabarina, was identified as trans-communic acid based on its spectroscopic properties and comparison with literature data [29]. Even though trans-communic acid (3) has been obtained from various natural sources [30], it was first isolated from Juniperus communis L. (Cupressaceae) [31]. The structure and configuration of this compound were established by extensive chemical transformations of the natural acid, its sodium salt and derivatives, as well as by correlation with labdane-type diterpenes of known configuration such as torulosol and manool [16]. In 1987 Shie-Ming Peng et al. confirmed the structure and relative configuration by Xray diffraction study of the methyl ester obtained from trans-communic acid (3), isolated from fresh leaves of Calocedrus formosana Florin [32]. In this study, meticulous attempts to crystallize transcommunic acid (3) were successful and crystals of the natural product which were suitable for X-ray diffraction were obtained. Figure 6 shows the computer-generated projection of the natural enantiomer of trans-communic acid (3). The absolute configuration was confirmed by calculating the Flack parameter whose value, x = 0.1 (3), confirms the absolute configuration shown in structure 3. Based on the above data the absolute configuration of trans-communic acid (3) was stablished as 4S5R9S10R. It is important to point out that labdane diterpenes have been isolated from several other species of the genus Salvia, such as Salvia sclarea L., S. officinalis L., S. palaestina Benth., S. aethiopis L., S. yosgadensis Freyn and Bornm [33], S. leriaefolia Benth. [34], S. rhytidea Benth. [35] and S. reuterana Boiss [36] which grow in Europe or the Middle East. This is the first time that labdane type diterpenoids have been isolated from a sage of the subgenus Calospahce. It is also important to mention that previous studies of S. cinnabarina (cultivated material) do not describe this type of diterpenes. Compound 3, isolated from this population of S. cinnabarina, was identified as trans-communic acid based on its spectroscopic properties and comparison with literature data [29]. Even though trans-communic acid (3) has been obtained from various natural sources [30], it was first isolated from Juniperus communis L. (Cupressaceae) [31]. The structure and configuration of this compound were established by extensive chemical transformations of the natural acid, its sodium salt and derivatives, as well as by correlation with labdane-type diterpenes of known configuration such as torulosol and manool [16]. In 1987 Shie-Ming Peng et al. confirmed the structure and relative configuration by X-ray diffraction study of the methyl ester obtained from trans-communic acid (3), isolated from fresh leaves of Calocedrus formosana Florin [32]. In this study, meticulous attempts to crystallize trans-communic acid (3) were successful and crystals of the natural product which were suitable for X-ray diffraction were obtained. Figure 6 shows the computer-generated projection of the natural enantiomer of trans-communic acid (3). The absolute configuration was confirmed by calculating the Flack parameter whose value, x = 0.1 (3), confirms the absolute configuration shown in structure 3. Based on the above data the absolute configuration of trans-communic acid (3) was stablished as 4S5R9S10R. Compound 3, isolated from this population of S. cinnabarina, was identified as trans-communic acid based on its spectroscopic properties and comparison with literature data [29]. Even though trans-communic acid (3) has been obtained from various natural sources [30], it was first isolated from Juniperus communis L. (Cupressaceae) [31]. The structure and configuration of this compound were established by extensive chemical transformations of the natural acid, its sodium salt and derivatives, as well as by correlation with labdane-type diterpenes of known configuration such as torulosol and manool [16]. In 1987 Shie-Ming Peng et al. confirmed the structure and relative configuration by Xray diffraction study of the methyl ester obtained from trans-communic acid (3), isolated from fresh leaves of Calocedrus formosana Florin [32]. In this study, meticulous attempts to crystallize transcommunic acid (3) were successful and crystals of the natural product which were suitable for X-ray diffraction were obtained. Figure 6 shows the computer-generated projection of the natural enantiomer of trans-communic acid (3). The absolute configuration was confirmed by calculating the Flack parameter whose value, x = 0.1 (3), confirms the absolute configuration shown in structure 3. Based on the above data the absolute configuration of trans-communic acid (3) was stablished as 4S5R9S10R. It is important to point out that labdane diterpenes have been isolated from several other species of the genus Salvia, such as Salvia sclarea L., S. officinalis L., S. palaestina Benth., S. aethiopis L., S. yosgadensis Freyn and Bornm [33], S. leriaefolia Benth. [34], S. rhytidea Benth. [35] and S. reuterana Boiss [36] which grow in Europe or the Middle East. This is the first time that labdane type diterpenoids have been isolated from a sage of the subgenus Calospahce. It is also important to mention that previous studies of S. cinnabarina (cultivated material) do not describe this type of diterpenes. It is important to point out that labdane diterpenes have been isolated from several other species of the genus Salvia, such as Salvia sclarea L., S. officinalis L., S. palaestina Benth., S. aethiopis L., S. yosgadensis Freyn and Bornm [33], S. leriaefolia Benth. [34], S. rhytidea Benth. [35] and S. reuterana Boiss [36] which grow in Europe or the Middle East. This is the first time that labdane type diterpenoids have been isolated from a sage of the subgenus Calospahce. It is also important to mention that previous studies of S. cinnabarina (cultivated material) do not describe this type of diterpenes.
Phthalic acid esters, derived from the esterification of phtalic acid (9) with long chain alcohols, have been isolated from various plant sources, for example, Ajuga bracteosa Wall. ex Benth. (Lamiaceae) [54], Hedyotis uncinella Hook. and Arn. (Rubiaceae) [55] and Phyllantus rheedii Wight. (Euphorbiaceae) [56] and also from marine organisms such as the red algae Acantophora spicifera (M. Vahl) Børgesen (Rhodomelaceae) [57]. Free phthalic acid is a product of the degradation of its esters by the action of some bacteria and it is known that it can have harmful effects by promoting the formation of reactive oxygen species causing cellular damage as described in Malus prunifolia (Willd.) Borkh [58]. Laboratory experiments determined that wheat, corn and soybean plants are capable of incorporating phthalic acid when the seeds are germinated in soil where this compound has been added [59]. Thus, the presence of 9 in S. cinnabarina raises the question of whether it is genetically part of the chemical composition, or whether this species incorporated phthalic acid from the soil where it grew.
The last aromatic compound was identified, based on its spectroscopic characteristics, as a mixture of esters of fatty acids with tyrosol. However, due to the low amount isolated, it was not possible to establish the size of the fatty acid chains. HPLC-MS analysis (Q-TOF) indicates the presence of a mixture of tyrosol fatty acids esters of more than 30 carbon atoms. These types of tyrosol derivatives have been previously isolated from different plant species [60,61] including a population of Salvia microphylla cultivated in Turkey [46]. Fatty acids esters of tyrosol had not been previously described in the chemical and biological analyses of Mexican sage.

Inhibition of α-Glucosidase Activity
Malonylcommunol (1), 6β-hydroxy-trans-communic acid (2) and trans-communic acid (3) were evaluated as α-glucosidase inhibitors in yeast and mammalian α-glucosidases. In a primary screening, using yeast α-glucosidase compounds 1-3 showed the highest inhibition of the enzyme, however the activity of the compounds decreased significantly in the mammalian α-glucosidase, evidencing a high specificity towards yeast α-glucosidase. Figure 7 shows that the effect of the evaluated compounds in yeast α-glucosidase is dependent of the concentration. Malonylcommunol (1) was the most active compound (IC 50 20.96 ± 0.58 µM), close to the reference compound quercetin (IC 50 16 Vahl) Børgesen (Rhodomelaceae) [57]. Free phthalic acid is a product of the degradation of its esters by the action of some bacteria and it is known that it can have harmful effects by promoting the formation of reactive oxygen species causing cellular damage as described in Malus prunifolia (Willd.) Borkh [58]. Laboratory experiments determined that wheat, corn and soybean plants are capable of incorporating phthalic acid when the seeds are germinated in soil where this compound has been added [59]. Thus, the presence of 9 in S. cinnabarina raises the question of whether it is genetically part of the chemical composition, or whether this species incorporated phthalic acid from the soil where it grew. The last aromatic compound was identified, based on its spectroscopic characteristics, as a mixture of esters of fatty acids with tyrosol. However, due to the low amount isolated, it was not possible to establish the size of the fatty acid chains. HPLC-MS analysis (Q-TOF) indicates the presence of a mixture of tyrosol fatty acids esters of more than 30 carbon atoms. These types of tyrosol derivatives have been previously isolated from different plant species [60,61] including a population of Salvia microphylla cultivated in Turkey [46]. Fatty acids esters of tyrosol had not been previously described in the chemical and biological analyses of Mexican sage.

Inhibition of α-Glucosidase Activity
Malonylcommunol (1), 6β-hydroxy-trans-communic acid (2) and trans-communic acid (3) were evaluated as α-glucosidase inhibitors in yeast and mammalian α-glucosidases. In a primary screening, using yeast α-glucosidase compounds 1-3 showed the highest inhibition of the enzyme, however the activity of the compounds decreased significantly in the mammalian α-glucosidase, evidencing a high specificity towards yeast α-glucosidase. Figure 7 shows that the effect of the evaluated compounds in yeast α-glucosidase is dependent of the concentration. Malonylcommunol

Plant Material
Salvia cinnabarina Martens and Galeotti was collected in Zoquitlan, State of Puebla, Mexico, in December 2017. Coordenates: 18 • 20 3.6 N, 96 • 59 37.5 W. Plant material was identified by Dr. Martha Martínez-Gordillo, and a voucher specimen (FCME 161531) was deposited at the Herbarium (FCME) of the Facultad de Ciencias (Mexico City, Mexico), UNAM. A photograph of the voucher is included as supplementary material.

Computational Details
3D models for compounds 3 and 4 were built, and geometry optimized using Spartan 10 utilizing a Merck Molecular Force Field (MMFF). Conformational analysis was performed with the same software under a PM3 semiempirical force field. The resulting conformers were filtered and checked for redundancy. All conformers within 4 kcal/mol were minimized, optimized and the thermochemical properties, IR and vibrational frequencies calculated using the DFT-B3LYP/DGDZVP force field in Gaussian 09. The TD-SCF with the default solvent model was used to perform the ECD calculations of the major conformers in MeOH solution at the B3LYP/6-31G* (d) level of theory. The calculated excitation energy (nm) and rotatory strength (R) in dipole velocity (R vel ) form were simulated into an ECD curve using the Harada-Nakanishi equation (Equation (1)) as implemented in the SpecDis software [63][64][65]. All calculations were performed on the HP Cluster Platform 3000SL "Miztli." 3.5. Single-Crystal X-ray Diffraction Analysis for Trans-Communic Acid (3) A colorless crystal was selected for experimental diffraction and mounted in a D8 venture K geometry diffractometer (Bruker AXS GmbH, Karlsruhe, Germany) with micro-focus X-ray source Cu kα radiation (λ = 1.54178 Å). The detector was placed at 50 mm from the crystal. Frames were collected with a scan width of 0.3 • in the ω scan and the exposure time of 10 sec/frame at 298 K. Frames were integrated with the Bruker SAINT software package (Bruker AXS Inc., Madison, Wisconsin, USA) using a narrow-frame integration algorithm. Systematic absences and intensity statistics were used in system orthorhombic, space group P2 1 2 1 2 1 . The structure was solved using direct methods using SHELXS-2014/7 program [66]. Hydrogen atoms were input at calculated positions and allowed to ride on the atoms to which they are attached. Thermal parameters were refined for hydrogen atoms on the aromatic ring and methylene using a Ueq = 1.2 Å and a Ueq = 1.5 Å for methyl groups to precedent atom in all cases. The final cycle of refinement was carried out on all non-zero data using SHELXL-2014/7 [66]. The Flack parameter was determined as 0.1(1) and confirmed with Bayesian parameters [67] P2 (true) = 1.000, P3 (false) = 0.3E-2 and Pearson z= 0.38 (11).
The trans-communic acid (3) was determined with two molecules crystallographically independent with an labdane-type structure, in according with Cremer  Crystallographic data (excluding structure factors) were deposited at the Cambridge Crystallographic Data Centre (CCDC) under the reference numbers CCDC 1983658, and copies of the data can be obtained free of charge upon application to the CCDC, 12 Union Road, Cambridge CB2 IEZ, UK. Fax: +44-(0)1223-336033 or e-mail: deposit@ccdc.cam.ac.uk.

TPA-Induced Edema Model
Animals. Male CD-1 mice weighing 25-30 g were maintained under standard laboratory conditions in the animal house (temperature 24 ± 2 • C) in a 12/12 h light−dark cycle, being fed laboratory diet and water ad libitum, following the Mexican official norm NOM-062-Z00-1999. The experimental procedures were approved by Internal Ethic Committee (CICUAL-IQ-004-17).
The TPA-induced ear edema assay in mice was performed as reported [69]. A solution of TPA (2.5 µg) in ethanol (10 µL) was applied topically to both faces (5 µL each ear) of the right ear of the mice, after 10 min the solutions of the test substances in their respective solvents were applied (10 µL each face). The left ear received ethanol (10 µL) then 20 µL of the respective solvent. The mice were killed with CO 2 four hours later. A 7 mm diameter plug was removed from each ear. The swelling was assessed as the difference in weight between the left and the right ear. Control animals received the correspondent solvent in each case. Edema inhibition (EI%) was calculated by the equation EI% = 100 − (B × 100/A), where A is the edema induced by TPA alone and B is the edema induced by TPA plus sample. Indomethacin and celecoxib were used as reference compounds.

Mammalian α-Glucosidadse Inhibition Assay
Mammalian α-glucosidase was prepared following the modified method of Jo [72]. Rat-intestinal acetone powder (100 mg) was rehydrated with 4 mL of 67 mM ice cold phosphate buffer (pH 6.8). After homogenized in an OMNI International Tissue Homogenizer (Omni International, Inc., Kennesaw, GA, USA) (125 model) for 3 min at 4 • C, the suspension was centrifuged (13,400 rcf, 4 • C, 30 min) and the resulting supernatant was used for the assay. A reaction mixture containing 175 µL phosphate buffer (67 mM, pH 6.8), 25 µL of α-glucosidase supernatant and 25 µL of sample at different concentrations (dissolved in DMSO 50%), was pre-incubated for 10 min at 37 • C. Then 25 µL of 23.2 mM PNP-G was added as a substrate. After further incubation of 15 min at 37 • C, the reaction was stopped whit 50 µL of Na 2 CO 3 (1 M). Acarbose and miglitol were used as a positive control and DMSO 5% as negative control. Enzyme activity was quantified by measuring the absorbance at 405 nm in a BioTek microplate reader Synergy HT (BioTek Instruments, Winooski, VT. USA). Experiments were done in triplicates. The percentage of enzyme inhibition by the sample was calculated by the following formula: % Inhibition = [(AC − AS) / AC)] × 100, where AC is the absorbance of the negative control and AS is the absorbance of the tested sample. The concentration of an inhibitor required to inhibit 50% of enzyme activity under the mentioned assay conditions is defined as the inhibition concentration 50 (IC 50 )

Conclusions
From the dichloromethane extract of a wild population of Salvia cinnabarina, several natural products were isolated, including two unpublished labdane-type diterpenoids named malonylcommunol (1) and 6β-hydroxy-trans-communic acid (2). Two already known labdane diterpenoids, trans-communic acid (3) and trans-communol (4) were also isolated and identified by spectroscopic means and comparison with literature date. Two isopimarane-type diterpenoids 5 and 6 were isolated together with two eudesmane-type sesquiterpenoids identified as β-eudesmol (7) and cryptomeridiol (8). Three aromatic natural products identified as phtalic acid (9), tyrosol derivatives (10) and the flavone salvigenin (11) were also isolated from this plant. Compounds 5 and 6 could be considered as biogenetic precursor of compound 12, a bioactive seco-isopimarane diterpenoid previously isolated from a cultivated population of S. cinnabarina. This work represented the first phytochemical analysis of a wild population of this plant.
Some products were tested in the TPA induced edema, anti-inflammatory assay with no significant results. The assay of compounds 1-3 as α-glucosidase inhibitors indicated high specificity towards yeast α-glucosidase. Malonylcommunol (1) was the most active compound (IC 50 20.96 ± 0.58 µM), near to the reference compound quercetin.