The Callus of Phaseolus coccineus and Glycine max Biotransform Flavanones into the Corresponding Flavones

In vitro plant cultures are gaining in industrial importance, especially as biocatalysts and as sources of secondary metabolites used in pharmacy. The idea that guided us in our research was to evaluate the biocatalytic potential of newly obtained callus tissue towards flavonoid compounds. In this publication, we describe new ways of using callus cultures in the biotransformations. In the first method, the callus cultures grown on a solid medium are transferred to the water, the reaction medium into which the substrate is introduced. In the second method, biotransformation is carried out on a solid medium by growing callus cultures. In the course of the research, we have shown that the callus obtained from Phaseolus coccineus and Glycine max is capable of converting flavanone, 5-methoxyflavanone and 6-methoxyflavanone into the corresponding flavones.


Figure S23
The HPLC chromatogram -7-days biotransformation of 5-methoxyflavanone (2) in Phaseolus coccineus callus water culture Figure S24 The UV absorption maxima of substrate (2) and product (2a) formed during 7-days biotransformation in Phaseolus coccineus callus water culture Figure S25 The HPLC chromatogram -14-days biotransformation of 5-methoxyflavanone (2) in Phaseolus coccineus callus water culture Figure S26 The UV absorption maxima of substrate (2) and product (2a) formed during 14-days biotransformation in Phaseolus coccineus callus water culture Figure S27 The HPLC chromatogram -7-days biotransformation of 6-methoxyflavanone (3) in Phaseolus coccineus callus water culture Figure S28 The UV absorption maxima of substrate (3) and product (3a) formed during 7-days biotransformation in Phaseolus coccineus callus water culture Figure S29 The HPLC chromatogram -14-days biotransformation of 6-methoxyflavanone (3) in Phaseolus coccineus callus water culture Figure S30 The UV absorption maxima of substrate (3) and product (3a) formed during 14-days biotransformation in Phaseolus coccineus callus water culture Figure S31 The HPLC chromatogram -7-days biotransformation of flavanone (1) in Glycine max callus water culture Figure S32 The UV absorption maxima of substrate (1) and product (1a) formed during 7days biotransformation in Glycine max callus water culture Figure S33 The HPLC chromatogram -14-days biotransformation of flavanone (1) in Glycine max callus water culture Figure S34 The UV absorption maxima of substrate (1) and product (1a) formed during 14-days biotransformation in Glycine max callus water culture Figure S35 The HPLC chromatogram -7-days biotransformation of 5-methoxyflavanone (2) in Glycine max callus water culture Figure S36 The UV absorption maxima of substrate (2) and product (2a) formed during 7-days biotransformation in Glycine max callus water culture Figure S37 The HPLC chromatogram -14-days biotransformation of 5-methoxyflavanone (2) in Glycine max callus water culture Figure S38 The UV absorption maxima of substrate (2) and product (2a) formed during 14-days biotransformation in Glycine max callus water culture Figure S39 The HPLC chromatogram -7-days biotransformation of 6-methoxyflavanone (3) in Glycine max callus water culture Figure S40 The UV absorption maxima of substrate (3) and product (3a) formed during 7-days biotransformation in Glycine max callus water culture Figure S41 The HPLC chromatogram -14-days biotransformation of 6-methoxyflavanone (3) in Glycine max callus water culture Figure S42 The UV absorption maxima of substrate (3) and product (3a) formed during 14-days biotransformation in Glycine max callus water culture Figure S43 The HPLC chromatogram -metabolites formed after 14 days in Phaseolus coccineus callus water culture (without substrate) Figure S44 The UV absorption maxima of Phaseolus coccineus metabolites

Figure S45
The HPLC chromatogram -metabolites formed after 14 days in Glycine max callus water culture (without substrate) Figure S46 The UV absorption maxima of Glycine max metabolites

Figure S2
The UV absorption maxima of flavanone (1

Figure S4
The UV absorption maxima of flavone (1a)

Figure S14
The UV absorption maxima of flavanone-substrate (1) and flavone-product (1a) formed during biotransformation in Phaseolus coccineus callus culture on solid medium

Figure S32
The UV absorption maxima of flavanone-substrate (1) and flavone-product (1a) formed during 7-days biotransformation in Glycine max callus water culture

Figure S34
The UV absorption maxima of flavanone-substrate (1) and flavone-product (1a) formed during 14-days biotransformation in Glycine max callus water culture

Figure S43
The HPLC chromatogram -metabolites formed after 14 days in Phaseolus coccineus callus water culture (without substrate)

Figure S44
The UV absorption maxima of Phaseolus coccineus metabolites

Figure S45
The HPLC chromatogram -metabolites formed after 14 days in Glycine max callus water culture (without substrate)

Figure S46
The UV absorption maxima of Glycine max metabolites