Cyclodextrin-Based Contrast Agents for Medical Imaging

Cyclodextrins (CDs) are naturally occurring cyclic oligosaccharides consisting of multiple glucose subunits. CDs are widely used in host–guest chemistry and biochemistry due to their structural advantages, biocompatibility, and ability to form inclusion complexes. Recently, CDs have become of high interest in the field of medical imaging as a potential scaffold for the development of a large variety of the contrast agents suitable for magnetic resonance imaging, ultrasound imaging, photoacoustic imaging, positron emission tomography, single photon emission computed tomography, and computed tomography. The aim of this review is to summarize and highlight the achievements in the field of cyclodextrin-based contrast agents for medical imaging.


Introduction
Cyclodextrins (CDs) are chemically stable naturally occurring cyclic oligosaccharides consisting of multiple glucose subunits connected by α-1,4 glycosidic bonds [1,2]. There are three main types of CDs that contain six (α-CD), seven (β-CD), and eight (γ-CD) glucose subunits in a ring ( Figure 1). These cyclodextrin macromolecules are cone-shaped with a hydrophobic interior cavity and polar exterior surface [3]. Due to their non-toxic nature [1,[4][5][6] and water solubility [7][8][9], CDs became widely used in various biomedical fields such as drug solubilization [7,[10][11][12], drug delivery [8,[13][14][15][16][17], and nucleic acid transfer [18][19][20]. Compared to other macrocyclic hosts, cyclodextrins are by far the most extensively used in hostguest chemistry applications and medical imaging [21][22][23][24][25]. They tend to be the macrocycle of choice due to their structural advantages and robust ability to form inclusion complexes [21,26]. An inclusion complex is formed when a guest molecule, commonly a small drug, is partially or fully encapsulated inside the host's interior cavity [1,3]. In the case of cyclodextrins, their preferred guest molecules tend to be hydrophobic, making them suitable for binding in the hydrophobic interior. Therefore, cyclodextrins possess the ability to form inclusion complexes with a wide variety of hydrophobic guest molecules [26][27][28]. Formation of inclusion complexes, or molecular encapsulation, can affect the physiochemical properties of the drug or molecule itself, such as solubility and rate of dissolution [3]. CDs are often exploited because of this property in addition to enhancing water solubility of water-insoluble molecules [3]. The exterior of cyclodextrin is predominantly hydrophilic due to the extensive hydrogen bonding network, making it a biocompatible agent for a wide range of applications [1][2][3][6][7][8][9][10][11][12][13][14][15][16][17]. These structural factors are largely why CDs are favored when synthesizing inclusion complexes. Electronics and thermodynamics both play a role in determining if a CD will form an inclusion complex with a guest molecule [1]. The driving force for inclusion complexation involves various noncovalent interactions such as desolvation, or removal, of water molecules from the interior cavity and formation of Van der Waals, hydrophobic, and hydrogen bonding interactions [29]. The major driving forces for cyclodextrin complexation are van der Waals interaction and hydrophobic interaction, whereas hydrogen bonding and electrostatic interaction mostly affect the conformation of a particular inclusion complex [29]. CD's ability to form inclusion complexes with small organic molecules has pioneered the field of supramolecular chemistry. More recently, CDs have successfully been used to build various molecular architectures such as catenanes, rotaxanes, pseudorotaxanes, polyrotaxanes, and other molecular machines [30,31].
During the past few decades, cyclodextrins have become of interest as contrast agents and potential biosensors for different medical imaging modalities [32]. In the context of imaging, CDs have been used primarily as a scaffold to support and/or solubilize smaller molecules that produce or quench a signal for enhanced imaging. However, in a few cases, the unique supramolecular nature of the CD is essential in producing the signal for imaging. Examples of CD-containing constructs that can be imaged by a wide variety of modern imaging technologies are discussed herein.
Magnetic resonance imaging (MRI) was the first imaging modality to utilize cyclodextrins as contrast agents [33]. CD-based MRI contrast agents produced contrast through the reduction in spinlattice relaxation (T1) time of the water protons. There are two different established methods of synthesis of CD-based MRI contrast agents: (1) host-guest interactions between CD cavity and metalorganic complexes [33,34] and (2) by direct conjugation of CDs to the metal-organic complexes through the external hydroxyl groups of CD molecule [35,36]. The reduction in T1 relaxation for CDbased contrast agents, and therefore their contrast, is substantially stronger compared to the metalorganic complexes on their own [34,37].
Secondly, positron emission computed tomography (PET) utilizes CD-based molecular imaging probes [25]. The PET probes emit positron, which annihilates with a stationary electron from the surroundings producing two gamma-photons, which are detected [38]. The PET tracers based on CD can be divided in two classes. The first class contains CD-based nanoparticles (NPs) radiolabeled with either 64 Cu [25] or 18 F [39]. Another recently developed type of CD-based PET imaging agents contains Compared to other macrocyclic hosts, cyclodextrins are by far the most extensively used in host-guest chemistry applications and medical imaging [21][22][23][24][25]. They tend to be the macrocycle of choice due to their structural advantages and robust ability to form inclusion complexes [21,26]. An inclusion complex is formed when a guest molecule, commonly a small drug, is partially or fully encapsulated inside the host's interior cavity [1,3]. In the case of cyclodextrins, their preferred guest molecules tend to be hydrophobic, making them suitable for binding in the hydrophobic interior. Therefore, cyclodextrins possess the ability to form inclusion complexes with a wide variety of hydrophobic guest molecules [26][27][28]. Formation of inclusion complexes, or molecular encapsulation, can affect the physiochemical properties of the drug or molecule itself, such as solubility and rate of dissolution [3]. CDs are often exploited because of this property in addition to enhancing water solubility of water-insoluble molecules [3]. The exterior of cyclodextrin is predominantly hydrophilic due to the extensive hydrogen bonding network, making it a biocompatible agent for a wide range of applications [1][2][3][6][7][8][9][10][11][12][13][14][15][16][17]. These structural factors are largely why CDs are favored when synthesizing inclusion complexes. Electronics and thermodynamics both play a role in determining if a CD will form an inclusion complex with a guest molecule [1]. The driving force for inclusion complexation involves various noncovalent interactions such as desolvation, or removal, of water molecules from the interior cavity and formation of Van der Waals, hydrophobic, and hydrogen bonding interactions [29]. The major driving forces for cyclodextrin complexation are van der Waals interaction and hydrophobic interaction, whereas hydrogen bonding and electrostatic interaction mostly affect the conformation of a particular inclusion complex [29]. CD's ability to form inclusion complexes with small organic molecules has pioneered the field of supramolecular chemistry. More recently, CDs have successfully been used to build various molecular architectures such as catenanes, rotaxanes, pseudorotaxanes, polyrotaxanes, and other molecular machines [30,31].
During the past few decades, cyclodextrins have become of interest as contrast agents and potential biosensors for different medical imaging modalities [32]. In the context of imaging, CDs have been used primarily as a scaffold to support and/or solubilize smaller molecules that produce or quench a signal for enhanced imaging. However, in a few cases, the unique supramolecular nature of the CD is essential in producing the signal for imaging. Examples of CD-containing constructs that can be imaged by a wide variety of modern imaging technologies are discussed herein.
Magnetic resonance imaging (MRI) was the first imaging modality to utilize cyclodextrins as contrast agents [33]. CD-based MRI contrast agents produced contrast through the reduction in spin-lattice relaxation (T 1 ) time of the water protons. There are two different established methods of synthesis of CD-based MRI contrast agents: (1) host-guest interactions between CD cavity and metal-organic complexes [33,34] and (2) by direct conjugation of CDs to the metal-organic complexes through the external hydroxyl groups of CD molecule [35,36]. The reduction in T 1 relaxation for CD-based contrast agents, and therefore their contrast, is substantially stronger compared to the metal-organic complexes on their own [34,37].
Secondly, positron emission computed tomography (PET) utilizes CD-based molecular imaging probes [25]. The PET probes emit positron, which annihilates with a stationary electron from the Molecules 2020, 25, 5576 3 of 27 surroundings producing two gamma-photons, which are detected [38]. The PET tracers based on CD can be divided in two classes. The first class contains CD-based nanoparticles (NPs) radiolabeled with either 64 Cu [25] or 18 F [39]. Another recently developed type of CD-based PET imaging agents contains CD molecules conjugated to the p-NCS-benzyl-NODA-GA (NODAGA) chelator labeled with 68 Ga [40,41]. Followed by PET, the CD-based contrast agents were developed for single photon emission computed tomography (SPECT). SPECT contrast agents were created by radiolabeling of CD-based NPs either with 99m Tc [42] or 125 I [43].
Recently, CDs were applied as contrast agents for ultrasound (US) and photo-acoustic imaging (PAI). The mechanism of contrast creation for US imaging relies on the substantial differences in the acoustic impedances between the biological tissue and the CD-based agents [44]. The mechanism of PA imaging is more complicated. The PA tracer absorbs the light with subsequent heating. Due to the temperature increase, the contrast agent undergoes thermoelastic expansion resulting in emission of the ultrasonic acoustic waves that can be detected by US receiver [45,46]. The developed CD photoacoustic contrast agents absorbed the light in the infrared range [47][48][49].
Lastly, multiple studies were conducted to evaluate the performance of the CD-based contrast agents for computed tomography (CT) [50][51][52]. All of the developments were focused on CD-based NPs that contained metal atoms (Au, Yb, Dy) [50][51][52][53]. The presence of the element with high atomic number rises up the X-ray absorption coefficient yielding to the contrast increase. These CD-based contrast agents demonstrated better performance compared to conventional iodine-based CT agents [50,51].
The purpose of this review is to provide an update on recent developments in CD-based contrast agents. A comparison of the developed contrast agents to the clinically available are presented as well as a comparison between different CD-based agents.

CD-Based Contrast Agents for MRI
MRI was the first medical imaging modality that began to develop CD-based contrast agents. The vast majority of the developed CD-based contrast agents reduce T 1 relaxation of the surrounding protons and were used to produce substantial contrast on T 1 -weighted images. Nevertheless, several studies demonstrated the potential of T 2 CD-based contrast agents as well. An overall summary of the achievements and developments in CD-based MRI contrast agents and molecular imaging probes is presented in detail below.
Due to the wide disfavor of traditional MRI probe modalities, mainly consisting of diethylenetriamine pentaacetic acid (DTPA) and dodecane tetraacetic acid (DOTA)-derived small molecule Gd(III) complexes, the continuous development and synthesis of innovative contrast agents is needed [54]. Cyclodextrin-based MRI contrast agents have gained notoriety in the last two decades and prove to be viable, robust candidates as potential MRI sensors for biomedical imaging [54]. In addition to their high biocompatibility and ability to form inclusion complexes, their large molecular weights, ease of functionalization and conjugation, and multivalent loading capacity make them well-suited as a new class of paramagnetic macromolecules [35,55,56]. CDs have proved to enhance contrast, sensitivity, and diagnostic imaging time [35]. This is attributed mainly due to their large molecular weights, which allow for longer and tunable rotational times [35,54].

In Vitro Studies of the Host-Guest CD-Based MRI Contrast Agents
The genesis of CD-based MRI contrast agents occurred in 1991 when Aime et al. [33] applied Freed's theory [57] to predict that the relaxation rates of the solvent protons increase when the paramagnetic ion or complex is bound to a macrocycle. This principle became a fundamental base for the future development of CD-based MRI contrast agents. Aime et al. reported numerous results for the inclusion complexes formation between CD and gadolinium chelates such as DOTA and DTPA. Host-guest interaction is dependent on internal cavity size: α-CD did not demonstrate host-guest interaction, while β-CD non-covalently bonded with DOTA/DTPA with significant increase in proton relaxivity in vitro (reciprocal spin-lattice relaxation time (1/T 1 )) observed for both β-CD-DOTA and β-CD-DTPA contrast agents [33]. Subsequent studies utilizing Gd(III)-bis(benzylamide)diethylenetriaminepentaacetic acid (BBA-DTPA) [34], gadolinium (III) 3,6,9,15-tetraazabi-cyclo [1,3,9]pentadeca-1(15),11,13-triene-3,6,9-triacetic acid (PCTA) complexed with poly-β-CD [58], and DOTA-benzyloxymethyl (DOTA(bom) 3 ) [59] demonstrated increasing water proton relaxivity due to the larger quantity of paramagnetic complexes accumulating in the region of interest due to interactions with a β-CD polymers [59]. The relaxivity of the developed poly-β-CD contrast agents exceeded up to six times the clinical analogues (r 1 = 61 mM −1 s −1 ) [59]. Furthermore, the results of modified Gd(III)-PCTA in blood serum suggested the possibility of application of this developed CD-based contrast agent in MRI angiography [58]. Despite such promising results, the relaxation measurements were conducted at 20 MHz NMR spectrometer at 0.5 T magnetic field far below the clinical MRI magnetic fields (1.5 or 3.0 T).
Larger CD-based NPs have also been the subject of recent research. These NPs have the potential for lower toxicity [60] compared to Gd-CD-based contrast agents. Examples of a proposed β-CD-based Gd-loaded NPs include a supramolecular assembly between DO3A-based gadolinium chelate (conjugated to adamantane through an acetamide spacer), poly-β-CD, and modified dextran [60] and cleavable β-CD-based Gd(III)-loaded nanocapsules, a promising agent as a redox-sensitive MRI contrast agent [61]. Besides toxicity reduction in Gd chelates, the proposed NPs are utilized the concept of further increasing of the number of Gd(III) atoms per unit of contrast agent resulting in increasing relaxivities.

In Vivo Imaging of the Host-Guest CD-Based Contrast Agents
The performance of some CD-based MRI imaging agents has been evaluated in vivo. Lahrech et al. demonstrated imaging of C6 glioma rats using a Gd-α-CD complex to quantify cerebral blood volume [62]. Although performance of Gd-α-CD contrast agents was significantly better compared to Gd-DOTA in terms of relaxation rates (r 1 = 7.3 mM −1 s −1 at 9.4 T), the developed supramolecular agent did not accumulate in tumors and almost no enhancement was observed on T 1 -weighted images during the first hour after Gd-α-CD injection. On the contrary, the CBV fraction was successfully measured using rapid steady state T 1 method and Gd-α-CD contrast agent.
More advanced CD-based contrast agents were demonstrated by Sun et al. who created a supramolecular complex between bridged bis(permethyl-β-cyclodextrin)s with Mn-porphyrin bearing polyethylene glycol side chains (Mn-TPP) [63]. Mice injected with this supramolecular polymer demonstrated strong contrast observed in the blood, kidneys, and bladder ( Figure 2) [63]. A supramolecular polymer built using the non-covalent interaction between Mn(II)-TPP and bridged tris(permethyl-β-CD)s resulted in a longitudinal relaxivity only 7% higher compared to previously developed Mn(II)-containing linear polymer [63,64].
Work by Feng et al. conducted on CD-based NPs as MRI contrast agents demonstrated neodymium doped NaHoF 4 NPs as T 2 imaging agents cultured with human mesenchymal stem cells injected into the brain hemisphere of nude mice. This combination of CD-based contrast agents and stem cells [23] supports the idea of using stem cells as an MRI contrast agent carrier. Furthermore, due to the high relaxivity of the developed probe (r 2 = 143.7 mM −1 s −1 at 11.7 T) [23], use of the developed contrast agent will be beneficial for ultrahigh field MRI imaging, since the transverse relaxivity increases highly with the magnetic field strength [23,65,66]. Work by Feng et al. conducted on CD-based NPs as MRI contrast agents demonstrated neodymium doped NaHoF4 NPs as T2 imaging agents cultured with human mesenchymal stem cells injected into the brain hemisphere of nude mice. This combination of CD-based contrast agents and stem cells [23] supports the idea of using stem cells as an MRI contrast agent carrier. Furthermore, due to the high relaxivity of the developed probe (r2 = 143.7 mM −1 s −1 at 11.7 T) [23], use of the developed contrast agent will be beneficial for ultrahigh field MRI imaging, since the transverse relaxivity increases highly with the magnetic field strength [23,65,66].

In Vivo Tumor Imaging
Imaging of cancer is one of the hot topics in modern medical imaging field. Despite the numerous developed contrast agents discussed above, only Zhou et al. used multiple β-CDs attached to a polyhedral oligomeric silsesquioxane nano globule at a targeted nano globular contrast agent

In Vivo Tumor Imaging
Imaging of cancer is one of the hot topics in modern medical imaging field. Despite the numerous developed contrast agents discussed above, only Zhou et al. used multiple β-CDs attached to a polyhedral oligomeric silsesquioxane nano globule at a targeted nano globular contrast agent from host-guest assembly for magnetic resonance cancer molecular imaging [67]. The host-guest contrast agent bonds to α v β 3 integrinin 4T1 malignant breast tumor through cyclic RGDfK peptide and gives greater contrast enhancement, due to the α v β 3 that is overexpressed in tumors (Figure 3a-c). This designed contrast agent produced superior contrast and signal enhancement compared to the clinically used Gd-based ProHance and non-targeted control cRAD-POSS-bCD-(DOTA-Gd)-Cy5 contrast agent. Molecular structure of cRGD-POSS-βCD-(DOTA-Gd)-Cy5 imaging agent is shown on Figure 3d.
Molecules 2020, 25, x FOR PEER REVIEW 6 of 27 from host-guest assembly for magnetic resonance cancer molecular imaging [67]. The host-guest contrast agent bonds to αvβ3 integrinin 4T1 malignant breast tumor through cyclic RGDfK peptide and gives greater contrast enhancement, due to the αvβ3 that is overexpressed in tumors (Figure 3ac). This designed contrast agent produced superior contrast and signal enhancement compared to the clinically used Gd-based ProHance and non-targeted control cRAD-POSS-bCD-(DOTA-Gd)-Cy5 contrast agent. Molecular structure of cRGD-POSS-βCD-(DOTA-Gd)-Cy5 imaging agent is shown on Figure 3d.

Direct Labeling of the CD Molecules
Another approach of synthesis of CD-based contrast agents for MRI imaging purposes is to conjugate metal-organic Gd(III)-containing complexes to the CD molecule through covalent bonds. We refer to this method as direct labeling of CD molecules. The key advantage of this approach is the

Direct Labeling of the CD Molecules
Another approach of synthesis of CD-based contrast agents for MRI imaging purposes is to conjugate metal-organic Gd(III)-containing complexes to the CD molecule through covalent bonds. We refer to this method as direct labeling of CD molecules. The key advantage of this approach is the availability of CD cavity for host-guest interaction with other molecules that can be effectively used for drug delivery study and imaging of molecular interactions.
Synthetically, modifying CDs in order to meet the criteria of an ideal contrast agent can be summarized by three key components: having a point of functionalization to attach the chelating group, designing a rigid linker in order to slow local movements, and lastly, conjugation of the macrocyclic complex in order to encapsulate multiple lanthanide chelates-thus, overall enhancing MRI signal, while improving overall stability and relaxivity profiles [35,[54][55][56]68].
Various synthetic approaches have been explored, which demonstrates CD's ability to be functionalized and conjugated in a robust fashion dependent on the desired application(s) [35,[54][55][56]68]. Bryson et al. synthesized a monodisperse β-CD Click cluster containing seven paramagnetic chelates encompassing two water exchange sites [35]. Using Click chemistry, an alkyne-functionalized dendron was reacted with the per-azido-β-CD to yield the desired product ( Figure 4). Using similar methods, Champagne et al. recently reported the synthesis of a different β-CD MRI probe containing seven iminodiacetate arms connected at the C6-position of β-CD by a triazole-based linker following a copper(I)-mediated 1,3-dipolar cycloaddition [54]. In addition, CDs can be differentially conjugated to produce multifunctional probes. Kotková et al. synthesized a novel bimodal fluorescence/MRI probe using a β-CD scaffold [55]. β-CD was labeled first using fluorescein isothiocyanate (FITC) and subsequentially with an isothiocyanate derivative containing a DOTA-based ligand [55]. The rigidity of the linker between the CD and the Gd-containing ligand plays an important role in increasing T R , thus enhancing the overall MRI signal [35,55]. Additionally, multifunctional NPs have been modified using an asymmetrically functionalized β-CD-based star copolymer by conjugating β-CD using doxorubicin (DOX), folic acid (FA), and DOTA-Gd moieties [54]. Similar to the work of Bryson et al. [35] and Champagne et al. [54], the key conjugation method used was azide-alkyne Huigsen cycloaddition, creating rigid triazole linkers.
Molecules 2020, 25, x FOR PEER REVIEW 7 of 27 availability of CD cavity for host-guest interaction with other molecules that can be effectively used for drug delivery study and imaging of molecular interactions. Synthetically, modifying CDs in order to meet the criteria of an ideal contrast agent can be summarized by three key components: having a point of functionalization to attach the chelating group, designing a rigid linker in order to slow local movements, and lastly, conjugation of the macrocyclic complex in order to encapsulate multiple lanthanide chelates-thus, overall enhancing MRI signal, while improving overall stability and relaxivity profiles [35,[54][55][56]68].
Various synthetic approaches have been explored, which demonstrates CD's ability to be functionalized and conjugated in a robust fashion dependent on the desired application(s) [35,[54][55][56]68]. Bryson et al. synthesized a monodisperse β-CD Click cluster containing seven paramagnetic chelates encompassing two water exchange sites [35]. Using Click chemistry, an alkynefunctionalized dendron was reacted with the per-azido-β-CD to yield the desired product ( Figure 4). Using similar methods, Champagne et al. recently reported the synthesis of a different β-CD MRI probe containing seven iminodiacetate arms connected at the C6-position of β-CD by a triazole-based linker following a copper(I)-mediated 1,3-dipolar cycloaddition [54]. In addition, CDs can be differentially conjugated to produce multifunctional probes. Kotková et al. synthesized a novel bimodal fluorescence/MRI probe using a β-CD scaffold [55]. β-CD was labeled first using fluorescein isothiocyanate (FITC) and subsequentially with an isothiocyanate derivative containing a DOTAbased ligand [55]. The rigidity of the linker between the CD and the Gd-containing ligand plays an important role in increasing TR, thus enhancing the overall MRI signal [35,55]. Additionally, multifunctional NPs have been modified using an asymmetrically functionalized β-CD-based star copolymer by conjugating β-CD using doxorubicin (DOX), folic acid (FA), and DOTA-Gd moieties [54]. Similar to the work of Bryson et al. [35] and Champagne et al. [54], the key conjugation method used was azide-alkyne Huigsen cycloaddition, creating rigid triazole linkers.

In Vitro Development
Skinner et al. demonstrated the first labeling of CD macrocycle with a Gd(III) chelate albeit the CD cavity was still used for non-covalent binding to another Gd(III) chelate in order to increase the proton relaxivity. [69]. Relaxivity of this complex increased when it was bound noncovalently to another gadolinium complex with the addition of two phenyl moieties.

In Vitro Development
Skinner et al. demonstrated the first labeling of CD macrocycle with a Gd(III) chelate albeit the CD cavity was still used for non-covalent binding to another Gd(III) chelate in order to increase the proton relaxivity. [69]. Relaxivity of this complex increased when it was bound noncovalently to another gadolinium complex with the addition of two phenyl moieties.
Bryson et al. created a contrast agent with a ten-fold increase in relaxivity at 9.4 T compared to clinically available Magnevist by labeling of per-azido-β-cyclodextrin core with seven diethylenetriaminetetraacetic acid (DTTA) Gd(III) chelates [35]. This development can be attributed to the increased Gd(III) ions per molecule and further increase in relaxivity due to conjugation to the macrocycle. In addition to the high relaxivity, unoccupied cavity of β-CD makes the developed contrast agent an excellent host scaffold to functionalize through noncovalent assembly with biological receptor-specific targets.
Bimodal MRI-fluorescence probes were demonstrated by Kotkova et al. [55] who combined a DOTA-based ligand with fluorescein functionality to simultaneously obtain fluorescence and MR images. Although the developed CD-based agent was studied in vitro, the benefit of this scaffold for MRI visualization under in vivo conditions was assumed due to its low cytotoxicity and high cell uptake. Fredy et al. developed cyclodextrin polyrotaxanes as a highly modular platform for an imaging agent [70]. Selectively functionalized cyclodextrins with a Gd(III) complex or BODIPY fluorescent tag were put on to a polyammonium chain to form polyrotaxanes. From this, polyrotaxanes could be assembled with fluorescent CDs and CDs with dia-or paramagnetic lanthanide complexes. Each threaded cyclodextrin was molecularly defined, which is an advantage over statistical post-functionalization of CD-polyrotaxanes. In vitro studies demonstrated that the Gd-bearing polyrotaxanes have relaxivities that are five times higher than Gd-DOTA, which makes them effective contrast agents for MRI applications [70].
NPs fabricated using biological macromolecules have been demonstrated by both Liu et al. [68] and Su et al. [71]. Liu designed pH disintegrable β-CD-based micellar NPs, while Su reported star-like dextran wrapped superparamagnetic iron oxide NPs. Both groups reported dual effects: an imaging contrast agent and cytotoxicity to HeLa cells at high concentrations, making these molecules both imaging probes and potential chemotherapeutics agents. Later, the synthesis of the contrast agent that affects the spin-spin (T 2 ) relaxation was suggested by conjugating β-CD to magnetic NPs [72].

In Vivo Imaging of CD-Based MRI Contrast Agents Based on the Direct Labeling of CD Cavity
In vitro studies have paved the way for a number of groups to demonstrate CD-based MRI contrast agents in vivo. Vascular imaging has long been a molecular imaging goal allowing investigators the ability to image the vasculature with high contrast. The G2/MOP-DTPA-Gd contrast agents synthesized from polyester dendrimers with β-CD core have been demonstrated with high yield and fast synthesis while providing for 2.7 times the relaxivity of Magnevist (DTPA-Gd) [24]. This contrast agent is rapidly hydrolyzed at the pH of 7.4 in the presence of esterase and slowly hydrolyzed at an acidic pH. The superior signal enhancement in vivo was observed following 0.1 mM Gd/kg injection ( Figure 5a) and was significantly higher compared to Magnevist (Figure 5b) Furthermore, the G2/MOP-DTPA-Gd contrast agent (Figure 5c) did not show tissue retention, making the ideal blood pool and kidney imaging agent.

In Vivo Imaging of Cancer
Cancer metastasis is the final insult leading to patient mortality. The early detection of metastasis is therefore a primary concern in the field of oncology. CD-based MRI contrast agents hold promise for the imaging of metastasis. Although CD-based contrast agents have yet been clinically tested, a number of groups have demonstrated these contrast agents in mammalian models.
Zhou et al. [74] reported the enhancement of MRI of liver metastases with a zwitterionized biodegradable dendritic CD-based contrast agent. Traditionally, the sensitivity in the liver for MR imaging of metastases is low due to the accumulation of the contrast agent in the Kupffer cells and hepatocytes instead of cancer cells. Zhou

Cyclodextrin-Based Contrast Agents for Hyperpolarized MRI
Although molecular imaging using MRI is challenging due to the lack of sensitivity of this imaging modality [78,79], progress has been made with the development of hyperpolarized (HP) MRI [79,80]. HP MRI utilizes the advantage of a metastable state with spin population excess significantly larger compared to the thermal equilibrium state [81]. Hyperpolarization of noble gases (such as 3 He and 129 Xe) is conducted via spin exchange optical pumping (SEOP), whereas polarization of 13 C or 1 H containing molecules is created through dynamic nuclear polarization (DNP) [82][83][84][85].

Hyperpolarized 13 C CD-Based Contrast Agents
Due to the high signal enhancement, molecular imaging using HP 13 C-containing molecule becomes possible. β-CD was used to create a contrast for HP 13 C MRI [86]. Keshari et al. showed that the host-guest interaction between a β-CD cavity and HP benzoic acid substantially decrease the T 1 relaxation of both HP 13 C nuclei resulting in negative image contrast (decrease in signal intensity) induced by the presence of supramolecular cage (Figure 7). Based on this result, the authors proposed that similar mechanism of the negative contrast can be used to study the interaction of ligand-receptor pairs in vivo.
Molecules 2020, 25, x FOR PEER REVIEW 11 of 27 relaxation of both HP 13 C nuclei resulting in negative image contrast (decrease in signal intensity) induced by the presence of supramolecular cage (Figure 7). Based on this result, the authors proposed that similar mechanism of the negative contrast can be used to study the interaction of ligandreceptor pairs in vivo. Relative MRI 13 C signal dependence on β-CD concentration [86]. The images are reprinted with permission from publisher [86].
DNP was used to create a CD contrast agent for DNP HP MRI [87]. Caracciolo et al. observed the polarization level of 10%. Unfortunately, the T1 relaxation of β-CD protons was equal to 1s at 300 K, which made HP β-CD inapplicable for molecular imaging purposes. On the other hand, HP β-CD can be of interest in the fields, which require the production of strong 1 H NMR signal from CD molecules [87]. Following this work was the hyperpolarization of methylated β-CD [88]. The methylation has been conducted using 13 CH3I, which enriched the potential contrast agent with 13 C and 1 H nuclei. Methylated β-CDs underwent DNP and polarization levels of 7.5 and 7% were achieved for 1 H and 13 C, respectively. The proton T1 relaxation times were found to be similar to those published in [87]; however, the T1 relaxation time of 13 C nuclei was equal to 3.3 and 4.9 s for fully methylated β-CDs and partially methylated β-CDs, respectively. These relaxation times allow further application of HP β-CD as contrast agents in the molecular imaging field. In addition, authors demonstrated the method of further increasing relaxation times [88].

CD-Based Molecular Probes for Hyperpolarized 129 Xe MRI
Current studies of molecular imaging with HP 129 Xe MRI utilizes hyperpolarized chemical exchange saturation transfer (HyperCEST) [89,90]. The HyperCEST effect relies on a constant chemical exchange between the dissolved HP 129 Xe nuclei in the solution and supramolecular host that can effectively encapsulate 129 Xe [79]. Following selective depolarization of the 129 Xe nuclei encapsulated in the supramolecular cage, the decrease in the dissolved phase 129 Xe MRI signal can be observed as a result of the exchange dynamic [79,89].
The interaction between HP 129 Xe and the α-CD cavity was studied for the first time in 1997 [91]. Authors observed that spin polarization induced nuclear Overhauser effect (SPINOE) and related transfer of nuclear polarization to the α-CD protons [91]. However, this approach did not become widely used in the field of molecular imaging using HP 129 Xe.
CD-based contrast agents for HyperCEST molecular imaging became of interest recently. The first detection of HyperCEST effect using α-CD-based molecules was achieved from the α-CD pseudorotaxane complex with five carbon diethylimidazolium bar in aqueous solution [92]. The DNP was used to create a CD contrast agent for DNP HP MRI [87]. Caracciolo et al. observed the polarization level of 10%. Unfortunately, the T 1 relaxation of β-CD protons was equal to 1s at 300 K, which made HP β-CD inapplicable for molecular imaging purposes. On the other hand, HP β-CD can be of interest in the fields, which require the production of strong 1 H NMR signal from CD molecules [87]. Following this work was the hyperpolarization of methylated β-CD [88]. The methylation has been conducted using 13 CH 3 I, which enriched the potential contrast agent with 13 C and 1 H nuclei. Methylated β-CDs underwent DNP and polarization levels of 7.5 and 7% were achieved for 1 H and 13 C, respectively. The proton T 1 relaxation times were found to be similar to those published in [87]; however, the T 1 relaxation time of 13 C nuclei was equal to 3.3 and 4.9 s for fully methylated β-CDs and partially methylated β-CDs, respectively. These relaxation times allow further application of HP β-CD as contrast agents in the molecular imaging field. In addition, authors demonstrated the method of further increasing relaxation times [88].

CD-Based Molecular Probes for Hyperpolarized 129 Xe MRI
Current studies of molecular imaging with HP 129 Xe MRI utilizes hyperpolarized chemical exchange saturation transfer (HyperCEST) [89,90]. The HyperCEST effect relies on a constant chemical exchange between the dissolved HP 129 Xe nuclei in the solution and supramolecular host that can effectively encapsulate 129 Xe [79]. Following selective depolarization of the 129 Xe nuclei encapsulated in the supramolecular cage, the decrease in the dissolved phase 129 Xe MRI signal can be observed as a result of the exchange dynamic [79,89].
The interaction between HP 129 Xe and the α-CD cavity was studied for the first time in 1997 [91]. Authors observed that spin polarization induced nuclear Overhauser effect (SPINOE) and related transfer of nuclear polarization to the α-CD protons [91]. However, this approach did not become widely used in the field of molecular imaging using HP 129 Xe.
CD-based contrast agents for HyperCEST molecular imaging became of interest recently. The first detection of HyperCEST effect using α-CD-based molecules was achieved from the α-CD pseudorotaxane complex with five carbon diethylimidazolium bar in aqueous solution [92]. The observed HyperCEST depletion was equal to 30%, which is significantly smaller compared to other molecular imaging probes [89,93]. The first β-CD-based molecular imaging probe for HyperCEST detection has been developed recently [94]. The HyperCEST contrast agent was realized as cucurbit [6]uril-based rotaxane in which β-CDs played the role of a stopper. Although rotaxane interaction with 129 Xe was through cucurbit [6]uril cavity and no HyperCEST effect was observed from β-CDs, the presence of β-CDs was required in order to increase the solubility of the end groups [94].
Finally, the HyperCEST effect from γ-CD-based pseudorotaxane was observed after the complexation of γ-CD with bisimidazolium guest [95]. Although γ-CD cavity is too large to sufficiently interact with HP 129 Xe, the cavity size can be decreased by threading it with a long alkyl chain. Threading these guest molecules through the cavity of γ-CD reduces the cavity size in order for adequate HP 129 Xe binding to occur, thus making it suitable for HyperCEST detection (Figure 8a). Potentially, this same concept can be applied for any supramolecular cage with a reasonably large cavity. The HyperCEST effect detected from γ-CD-based pseudorotaxanes was equal to 47.5% on average, which makes them interesting candidates for further application in vivo [95]. The main advantage of the HyperCEST contrast agents based on pseudorotaxane architecture over the other studied HP 129 Xe hosts is the ease synthesis and of functionalization of the pseudorotaxanes. Finally, the HyperCEST effect from γ-CD-based pseudorotaxane was observed after the complexation of γ-CD with bisimidazolium guest [95]. Although γ-CD cavity is too large to sufficiently interact with HP 129 Xe, the cavity size can be decreased by threading it with a long alkyl chain. Threading these guest molecules through the cavity of γ-CD reduces the cavity size in order for adequate HP 129 Xe binding to occur, thus making it suitable for HyperCEST detection (Figure 8a). Potentially, this same concept can be applied for any supramolecular cage with a reasonably large cavity. The HyperCEST effect detected from γ-CD-based pseudorotaxanes was equal to 47.5% on average, which makes them interesting candidates for further application in vivo [95]. The main advantage of the HyperCEST contrast agents based on pseudorotaxane architecture over the other studied HP 129 Xe hosts is the ease synthesis and of functionalization of the pseudorotaxanes. In addition, γ-CD pseudorotaxane complexes prove to be a sufficient paradigm for HP 129 Xe MRI. The synthesis of the threads proceeds in one step and can be functionalized with different terminal end groups as well as different chain lengths. The 8-and 10-carbon alkyl chains were functionalized with ethylimidazoliums (Figure 8b), strategically used to enhance water solubility of the inherently hydrophobic alkyl chain [95]. The γ-CD pseudorotaxane was comparable to that of CB6, a known xenon cage that has been responsive to in vivo HP 129 Xe MRI [80]. Similar to CB6, the maximum HyperCEST depletion for γ-CD pseudorotaxanes was present when the samples were In addition, γ-CD pseudorotaxane complexes prove to be a sufficient paradigm for HP 129 Xe MRI. The synthesis of the threads proceeds in one step and can be functionalized with different terminal end groups as well as different chain lengths. The 8-and 10-carbon alkyl chains were functionalized with ethylimidazoliums (Figure 8b), strategically used to enhance water solubility of the inherently hydrophobic alkyl chain [95]. The γ-CD pseudorotaxane was comparable to that of CB6, a known xenon cage that has been responsive to in vivo HP 129 Xe MRI [80]. Similar to CB6, the maximum HyperCEST depletion for γ-CD pseudorotaxanes was present when the samples were irradiated at a frequency of +128 ppm [95]. γ-CD pseudorotaxanes exhibited binding on the order of 10 3 at 1:1 host-guest complexation, proving to be sufficient association for HP 129 Xe. In addition, binding proved to be two magnitudes of order lower in fetal bovine serum, indicating that this system would work sufficiently as a potential biosensor in vivo [95].

CDs-Based Contrast Agent for Ultrasound Imaging and Photoacoustic Imaging
In addition to MRI contrast agents, CDs have been used for ultrasound (US) imaging. The first approach of synthetizing the potential contrast agent was developed by Cavalieri et al., in 2006 [96]. Air-filled polymer microbubbles functionalized with β-CDs were used as a source for contrast. The contrast originates from significant difference between acoustic impedance of tissue and air encapsulated inside of a microbubble. Cavalieri et al. found that conjugating microbubbles to β-CD preserves them from random coil to αhelix conformation transition. In addition, due to the presence of β-CD, these contrasts allow hosting molecules with hydrophobic features [96].
After nearly a decade of inactivity, the second attempt of using a β-CD-based contrast agent for US imaging was made in 2015 [44]. The authors demonstrated use of a perfluorinated FC-77/ β-CD complex. A visible blurring of signal from FC-77/β-CD caused by a disruption of the inclusion complexes under US was detected [44].
Another US medical imaging modality that utilized CD-based contrast agents was PAI. The first CD-based contrast agent was synthetized by surface modification of oleic-acid (OA) stabilized upconversional NPs (UCNPs) NaYF 4 :Yb 3+ , Er 3+ with α-CD [49]. α-CD formed an inclusion complexes with an OA yielding to luminescence quenching of UCNPs and production of a strong PA signal instead (Figure 9a). Cytotoxicity studies demonstrated no toxicity of α-CD/UCNPs contrast agents. Following in vitro imaging (Figure 9b), the first PAI in a living mouse was conducted using 980 nm excitation laser. These results demonstrated the ability of α-CD/UCNPs to be an efficient PAI contrast agent for diagnostic purposes [49].

CDs-Based Contrast Agent for Ultrasound Imaging and Photoacoustic Imaging
In addition to MRI contrast agents, CDs have been used for ultrasound (US) imaging. The first approach of synthetizing the potential contrast agent was developed by Cavalieri et al., in 2006 [96]. Air-filled polymer microbubbles functionalized with β-CDs were used as a source for contrast. The contrast originates from significant difference between acoustic impedance of tissue and air encapsulated inside of a microbubble. Cavalieri et al. found that conjugating microbubbles to β-CD preserves them from random coil to α-helix conformation transition. In addition, due to the presence of β-CD, these contrasts allow hosting molecules with hydrophobic features [96].
After nearly a decade of inactivity, the second attempt of using a β-CD-based contrast agent for US imaging was made in 2015 [44]. The authors demonstrated use of a perfluorinated FC-77/ β-CD complex. A visible blurring of signal from FC-77/β-CD caused by a disruption of the inclusion complexes under US was detected [44].
Another US medical imaging modality that utilized CD-based contrast agents was PAI. The first CD-based contrast agent was synthetized by surface modification of oleic-acid (OA) stabilized upconversional NPs (UCNPs) NaYF4:Yb 3+ , Er 3+ with α-CD [49]. α-CD formed an inclusion complexes with an OA yielding to luminescence quenching of UCNPs and production of a strong PA signal instead (Figure 9a). Cytotoxicity studies demonstrated no toxicity of α-CD/UCNPs contrast agents. Following in vitro imaging (Figure 9b), the first PAI in a living mouse was conducted using 980 nm excitation laser. These results demonstrated the ability of α-CD/UCNPs to be an efficient PAI contrast agent for diagnostic purposes [49]. Figure 9. (a) High-resolution PA signal originated from upconversional NPs (UCNPs) in cyclohexane (black curve), distilled water (green curve), and α-CD/UCNPs (red curve) in water. The excitation was conducted using 980 nm nanosecond pulsed laser. (b) Photo-acoustic imaging (PAI) of tissuemimicking phantom containing chambers filled with α-CD/UCN in water (green arrow) and distilled water (white arrow) [49]. The images are reprinted with permission from the publisher [49].
An effective PAI agent for imaging prosthetic joint infection (PJI) [48] was demonstrated by conjugation of β-CD to indocyanine green (ICG). The β-CD-ICG PAI agent was demonstrated in the mice model of PJI. Wang et al. found that conjugation of ICG to β-CD improves its PA signal generation. Although the PAI signal increase was not significant with β-CD-ICG contrast, it still demonstrated the ability to serve as a contrast agent for non-invasive diagnostic of PJI [48]. (a) High-resolution PA signal originated from upconversional NPs (UCNPs) in cyclohexane (black curve), distilled water (green curve), and α-CD/UCNPs (red curve) in water. The excitation was conducted using 980 nm nanosecond pulsed laser. (b) Photo-acoustic imaging (PAI) of tissue-mimicking phantom containing chambers filled with α-CD/UCN in water (green arrow) and distilled water (white arrow) [49]. The images are reprinted with permission from the publisher [49].
An effective PAI agent for imaging prosthetic joint infection (PJI) [48] was demonstrated by conjugation of β-CD to indocyanine green (ICG). The β-CD-ICG PAI agent was demonstrated in the mice model of PJI. Wang et al. found that conjugation of ICG to β-CD improves its PA signal generation. Although the PAI signal increase was not significant with β-CD-ICG contrast, it still demonstrated the ability to serve as a contrast agent for non-invasive diagnostic of PJI [48].
Yu et al. recently developed a CD-based PAI contrast agent sensitive to tumor environment [47]. Gold NPs (AuNP) were modified initially with pyridine-2-imine-terminated single stand DNA via gold-thiol bonds, and α-CDs were capped on the end of DNA through hydrophobic interaction with CD's cavity. The α-CD-AuNP agent produced no PA signal under neutral pH conditions, but upon entering the tumor, α-CDs separate from the DNA ends due to reduction in non-covalent forces. This study demonstrated that a developed α-CD-AuNP contrast agent can be successfully used as a tumor-selective theranostic agent [47].

Radiolabeled CD-Based Contrast Agents
PET and SPECT are imaging modalities that require probe radiolabeling to produce tomographic images. These modalities have superior sensitivity and deeper tissue penetration compared with luminescence-based imaging and MRI [97]; they require lower dose of imaging agent for functioning. However, the application of PET/SPECT tracers is limited to the half-life of radiolabeled isotopes and biodistribution in the living organism.
The first demonstration of the CD-based PET molecular imaging probe was done by Bartlett et al., in 2007 [25]. CD-containing NPs were studied as delivery agents for transferrin (Tf)-targeted delivery to tumors of siRNA molecules. Nontargeted and Tf-targeted siRNA NPs were synthetized by using cyclodextrin-containing polycation. One percent of the targeted NPs containing adamantane-PEG molecules on the surface were modified with Tf. Si-RNA were conjugated with DOTA to the 5 end with further 64 Cu labeling [25]. MicroPET revealed negligible impact of the attachment of the Tf targeting ligand to the NPs on biodistribution. Unfortunately, nearly identical tumor localization kinetics of both targeted and nontargeted 64 Cu-DOTA-siRNA NPs were observed; tumor accumulation was also similar at 1 day after injection (≈1% ID/cm 3 ). However, bioluminescent imaging showed the intracellular localization and functional activity of siRNA delivered by Tf-targeted NPs in the tumor cells 1 day after injection.
Interestingly, the presence of CD in therapeutic compounds allows radio isotopic labelling for PET imaging of the drug circulation. In vivo biodistribution of the IT-101, clinically developed drug for cancer treatment, in mice with Neuro2A tumors was studied by Schluep et al. [98]. IT-101 contains molecule drug camptothecin (CPT) conjugated with β-CD based polymers (CDP), which acts as a carrier system for active molecule. The CDP site was labeled with 64 Cu through attaching of DOTA complex for microPET imaging; the obtained nanoparticle was similar to the IT-101 structure. Plasma pharmacokinetics of 64 Cu-IT-101 ere studied at 1, 4, and 24 h after injection with microPET/CT. It was shown that a low-molecular-weight fraction cleared rapidly through kidneys due to biphasic elimination profile, whereas remaining NPs circulated with a terminal half-life of ≈13 h. Biodistribution of IT-101 was examined at 24 h after administration; the highest tissue concentration was found in the tumor followed by the liver.
Another study conducted to investigate the effect of CD inclusion in NPs for drug delivery revealed the possibility of CD-based SPECT imaging agent [42]. Areses et al. compared the adhesive abilities and biodistribution of orally administered poly(anhydride) NPs and CD containing NP (CD-NP) in rats utilizing labeling with 99m Tc for SPECT imaging. 99m Tc-NP showed activity only in the gastrointestinal tract on SPECT images, whereas 99m Tc-CD-NP revealed extended residence time in stomach: about 13% of 99m Tc-CD-NP administered dose and 3% of 99m Tc-NP given dose were found in the stomach after 8 h. Liu et al., in 2011 [39], studied the improvement of the biodistribution of NPs using CD. Rare-earth UCNPs were modified by α-CD and OA for increasing of the water-solubility. UCNP-OA-CD complexes with Tm inclusion were labeled with 18 F ( 18 F-UCNP(Tm)-OA-CD) for microPET imaging of ex vivo and in vivo biodistribution in mice at 5 min and 2 h. This was the first labeling of CD-based probe with an 18 F. Ex vivo imaging displayed rapid accumulation of NPs in the liver (~90.8% injected dose(ID)/g) and spleen (~62.5% ID/g) at 5 min and further decreasing of liver uptake to~57.6% ID/g, while increased spleen accumulation to 118.9% ID/g after 2 h post-injection. In vivo microPET images were consistent with ex vivo biodistribution results and showed intense radioactive signals in the liver and spleen at 5 min after injection.
In addition to the previously discussed reports, a pre-targeted approach for molecular imaging probe development was presented by Hou et al. [99]. This work was based on biorthogonal conjugation chemistry between NPs, which have tendency to accumulate in tumors to enhanced permeability and retention (EPR) effect and radiolabeled imaging agents for PET imaging. One of NPs components was synthesized from CD-grafted polyethyleneimine (CD-PEI) and trans-cyclooctene N-hydroxysuccinimide (TCO-NHS), resulted in TCO/CD-PEI building block. Actual tumor-targeting NP (TCO⊂SNPs) was prepared via self-assembly from four different blocks TCO/CD-PEI, CD-PEI, adamantane-grafted polyamidoamine (Ad-PAMAM), and Ad-grafted polyethylene glycol (Ad-PEG) and injected to the tail vein of the mice with U87 glioblastoma cells. After EPR-driven accumulation in tumor, TCOresulted in TCO/CD-PEI building block. Actual tumor-targeting NP (TCO⊂SNPs can dynamically disassemble to release TCO/CD-PEI. After 24 h post-injection of TCO⊂SNPs, freshly prepared tetrazine compound radiolabeled with 64 Cu through DOTA ( 64 Cu-Tz) was injected. Subsequently, distributed 64 Cu-Tz can undergo biorthogonal reaction with TCO/CD-PEI parts left after TCO⊂SNPs disassembling in vivo, yielding the dihydropyrazine conjugation adduct 64 Cu-DHP/CD-PEI, which acts as a contrast agent for tumors. Multiple microPET and anatomical CT images were acquired following the injection of pre-targeted NPs and 64 Cu-Tz compound with in vivo reaction (Figure 10a), along with two series of control PET imaging of a fully ex vivo prepared 64 Cu-DHP/CD-PEI adduct (Figure 10b) and free radiolabeled reporter 64 Cu-Tz (Figure 10c). Pre-targeted studies showed the accumulation and retention of radioactivity mainly in the glioblastoma tumor and liver and some nonspecific uptake by tissues. Supramolecular nanoparticles (SNP) control and probe ( 64 Cu-Tz) imaging did not present highly distinguishable tumor uptake. Although, high radioactivity was observed in the liver in all three cases, which can be explained by 64 Cu 2+ dissociation from DOTA ligand. This issue can be eliminated by using different radioisotopes for labelling.
β-CD-based rotaxane were used in developing theranostic shell-crosslinked NPs (SCNPs) by Yu et al. for improving drug delivery and controllable release in supramolecular medicine [102]. The core-shell-structured self-assembling NPs were obtained from polyrotaxanes consisted of amphiphilic diblock copolymer and the primary-amino-containing β-CD (β-CD-NH 2 ), which undergoes complexation with poly(ε-caprolactone) (PCL) segment. In the gained structure, amphiphilic deblock copolymer acts as the axle, and β-CD-NH 2 acts as a wheel in complex with PCL, whose chains can experience hydrophobic interactions along with the perylene diimide (PDI) stoppers, which has a tendency to π-π stacking interactions. Obtained SCNPs were labeled with radioactive 64 Cu through DOTA attachment to SCNPs ( 64 Cu SCNPs@DOTA) for PET imaging of the dynamic biodistributions and accumulations of SCNPs in the main organs. HeLa tumor-bearing mice were imaged at various time-points after intra-venous injection 150 µCi of 64 Cu SCNPs@DOTA. Images revealed the high liver uptake of 64 Cu SCNPs@DOTA along with increasing of tumor uptake from the point of injection to 12 h post-injection with further start of clearance at 48 h.
TCO/CD-PEI. After 24 h post-injection of TCO⸦SNPs, freshly prepared tetrazine compound radiolabeled with 64 Cu through DOTA ( 64 Cu-Tz) was injected. Subsequently, distributed 64 Cu-Tz can undergo biorthogonal reaction with TCO/CD-PEI parts left after TCO⸦SNPs disassembling in vivo, yielding the dihydropyrazine conjugation adduct 64 Cu-DHP/CD-PEI, which acts as a contrast agent for tumors. Multiple microPET and anatomical CT images were acquired following the injection of pre-targeted NPs and 64 Cu-Tz compound with in vivo reaction (Figure 10a), along with two series of control PET imaging of a fully ex vivo prepared 64 Cu-DHP/CD-PEI adduct (Figure 10b) and free radiolabeled reporter 64 Cu-Tz (Figure 10c). Pre-targeted studies showed the accumulation and retention of radioactivity mainly in the glioblastoma tumor and liver and some nonspecific uptake by tissues. Supramolecular nanoparticles (SNP) control and probe ( 64 Cu-Tz) imaging did not present highly distinguishable tumor uptake. Although, high radioactivity was observed in the liver in all three cases, which can be explained by 64 Cu 2+ dissociation from DOTA ligand. This issue can be eliminated by using different radioisotopes for labelling. Figure 10. Timeline of the injection protocol employed for (a) pre-targeted, (b) SNP control (64Cu-DHP⊂SNPs), and (c) free radiolabeled reporter (64Cu-Tz) studies. Representative in vivo Figure 10. Timeline of the injection protocol employed for (a) pre-targeted, (b) SNP control (64Cu-DHP⊂SNPs), and (c) free radiolabeled reporter (64Cu-Tz) studies. Representative in vivo microPET/CT images of the mice (n = 4/group) subjected to the three studies at 24 h p.i. Labels T, L, K, and B refer to the tumor, liver, kidney, and bladder, respectively. Dashed lines correspond to the transverse cross-section through the center of each tumor mass, whose image is shown in the right panel [99]. The images are reprinted with permission from publisher [99]. (d) The chemical structure of the tumor targeting imaging probe developed by Hou et al. [99].
Another study utilized PET for investigation in vivo distribution of 2-Hydroxypropyl-β-CD (HPBCD), β-cyclodextrin derivative, and an orphan drug for the Niemann-Pick disease treatment [40]. Six-deoxy-6-monoamino-(2-Hydroxypropyl)-β-CD (NH2-HPBCD) was conjugated with p-NCS-benzyl-NODA-GA (NODAGA) and radiolabeled with 68 Ga for PET/CT imaging. Ex vivo and in vivo studies on healthy mice showed that 68 Ga-NODAGA-HPBCD was mainly excreted through the urinary system with low uptake of the abdominal and thoracic organs and tissues at 30 and 90 min post-injection.
The most recent study in the area of CD-based PET molecular imaging probes containing tumor targeting compounds was done by Trencsenyi et al., in 2019 [41]. Their aims were to develop novel radiolabeled compound specific to the prostaglandin E2 (PGE2), which plays an important role in tumor progress and formation of metastases. The high affinity of PGE2 to the randomly methylated β-CD (RAMEB) was reported by Sauer et al. [103]. Trencsenyi et al., in their research, aimed to synthesize PGA-specific RAMEB labeled with 68 Ga through NODAGA ( 68 Ga-NODAGA-RAMEB) for investigation of its tumor-targeting properties and in vivo biodistribution using PET. PancTu-1 and BxPC3 tumor-bearing SCID mice were intravenously injected with 68 Ga-NODAGA-RAMEB. The injection was followed with dynamic and static microPET imaging at 0-90 min. The accumulation of 68 Ga-NODAGA-RAMEB was significantly higher in BxPC3 tumors than in the PancTu-1; the highest post-injection tumor-background ratio (T/M) was obtained at 80-90 min post-injection. The T/M standardized uptake values (SUVs) were 10-fold lower in the PancTu-1 than those of BxPC3 tumors confirming the high PGE2 selectivity of 68 Ga-lebeled cyclodextrin.

CD-Based CT Contrast Agents
The initial development of CD-based contrast agents for CT was used to investigate multimodality imaging potential of phosphorescent-modified NaDyF 4 NPs (DyNPs) [53]. OA-coated DyNPs underwent complexation with α-CD followed by conjugation with Gd(III)-DTPA complex. The last stage of contrast agent formation was the loading of phosphorescent Ir(Dbz) 2 (Pbi) complex with in the hydrophobic layer of Gd-α-CD-DyNPs [53]. Besides the contrast creation potential for fluorescence and MRI, Zhou et al. considered the developed Ir-Gd-α-CD-DyNPs as a potential contrast agent for CT due to the presence of heavy atoms of Dy, Gd, and Ir. The measured Hounsfield units (HU) value of 10 mg/mL Ir-Gd-α-CD-DyNPs aqueous solution was equal to 158 at 80 kV X-ray energies. Following in vitro experiments, Ir-Gd-α-CD-DyNPs were used for CT scanning of tumor-bearing mice ( Figure 11). The original HU value of 109 for the tumor increased to 212 HU after intratumoral injection of 100 ul of 3 mg/mL Ir-Gd-α-CD-DyNPs ( Figure 11G) solution. As a result, approximately 100% signal enhancement was observed on the CT scans.
Another multimodal CD-based contrast agent that was developed for CT scanning was a core-shell-structured alkali ion-doped CaF 2 :Yb,Er UCNP [50]. Similar to the NPs discussed above, the core of NPs was coated with OA with subsequent complexation with α-CD. The final α-CD/UCNPs contrast agent was compared to well-known iopromide 300 CT clinical contrast agent. Yin et al. found that developed α-CD/UCNPs have 45% higher HU value at concentration 80 mM/L. This high X-ray absorption originates from the present high atomic number elements. In addition, investigated α-CD/UCNPs were loaded with doxorubicin, and the ability of simultaneous cancer imaging and treatment using this contrast agent was suggested [50].
The next achievement in the CT contrast agent development was obtained in 2017 by conjugating β-CD to poly(methyl vinyl ether-alt-maleic anhydride) (PVME-alt-MAH) with a subsequent reaction using modified dextran to create gel microspheres [104]. To make dextran microspheres visible for CT, they were loaded with iodine in n-hexane. Through the micro-CT phantom imaging, Zhu et al. observed the subsequent improvement of contrast after iodine loading.
contrast agent for CT due to the presence of heavy atoms of Dy, Gd, and Ir. The measured Hounsfield units (HU) value of 10 mg/mL Ir-Gd-α-CD-DyNPs aqueous solution was equal to 158 at 80 kV X-ray energies. Following in vitro experiments, Ir-Gd-α-CD-DyNPs were used for CT scanning of tumorbearing mice (Figure 11). The original HU value of 109 for the tumor increased to 212 HU after intratumoral injection of 100 ul of 3 mg/mL Ir-Gd-α-CD-DyNPs ( Figure 11G) solution. As a result, approximately 100% signal enhancement was observed on the CT scans. Figure 11. In vivo 3D volume-rendered (A,B) and maximum intensity projections in axial (C,D) and coronal (E,F) view CT images of the tumor-bearing mouse obtained pre-(A,C,E) and post-(B,D,F) injection of Ir-Gd-α-CD-DyNPs contrast agent [53]. The images are reprinted with permission from the publisher [53]. The position of the tumor was marked by red circles. The chemical structure of the developed CT contrast agent is shown in (G). Another step in the development of CD-based CT contrast agents was done by creation an unimolecular micelle system synthesized from 21-arm star-like polymer β-CD-{poly(lactide)-poly(2-(dimethylamino) ethyl methacry late)-poly[oligo(2-ethyl-2-oxazoline) methacrylate]} 21 (β-CD-(PLA-PDMAEMA-PEtOxMA) 21 ) followed by production of β-CD-(PLA-PDMAEMA-PEtOxMA) 21 /AuNPs/DOX [105]. The developed Au-loaded β-CD-based contrast agent was tested in the animal HepG2 tumor model. Lin et al. found that intravenously injected β-CD-(PLA-PDMAEMA-PEtOxMA) 21 /AuNPs/DOX produces substantially higher CT contrast compared to iodine-based Omnipaque, which was used for control scans.
Following the previous studies, the most recent advance in the field of CD-based CT contrast agents was achieved by the same group [106].

Discussion
Starting in the early 1990s, CDs became of large interest for developing contrast agents and molecular probes for medical imaging. Although the primary application of CDs in medical imaging was the basis for developing novel Gd-based MRI contrast agents, CD-based contrast agents commence to be utilized by other imaging modalities such as PET, CT, US, and PAI. This growing interest is caused by excellent biocompatibility, low toxicity, and relative ease of modifying of CD molecules. Currently, the most frequently used CD molecule for contrast agent development is β-CD. Despite the extensive development of β-CD-based contrast agents, some of the medical imaging areas started utilizing αand γ-CD-based contrast agents and molecular probes as well.
The working mechanism of the vast majority of CD-based contrast agents for conventional MRI relies on the Freed's theory [57] and the decreasing of the relaxation times of the solvent protons once either paramagnetic ion or metal-organic complex containing the paramagnetic ion binds to a supramolecular cage. Initially, this binding was done through hydrophobic interaction between CDs cavity and metal-organic complexes. Although contrast agents synthesized using this approach demonstrated significantly better performance compared to the clinically available analogs, the main disadvantage of this technique is blocking the supramolecular cage cavity, which makes the developed contrast agents and molecular probes uncapable to image some drug transport, biodistribution, and treatment monitoring. In addition, the number of paramagnetic ions per molecule is also limited, since usually only one metal-organic complex enters the cavity. This issue was overcome by creating different CD-based polymers and dendrimers, and the number of macromolecules determined the maximum number of paramagnetic ions per molecule. Starting from the early 2000s, the direct labeling of the CD macrocycles with chelates containing paramagnetic ions has been developed intensively. This method allows keeping the CD cavity free for interactions with different molecules. Therefore, the contrast agents based on the direct labeling of the CD macromolecules have a significantly larger field of applications compared to agents developed using non-covalent interactions.
Currently, CD-based MRI contrast agents contain mostly Gd(III) ions and are designed as T 1 contrast agents. On the other hand, the development of CD-based T 2 and T 2 * contrast agents have been demonstrated in vitro and in vivo [23,71,75]. Although some of the developed CD-based contrast agents for proton MRI demonstrated an overall low level of cytotoxicity, dedicated toxicology studies are needed prior to the further translation of this imaging agents into clinics. In addition, the vast majority of the relaxivity measurements were conducted at low magnetic fields, which are not for clinical imaging purposes, such as 0.47 and 0.5 T. The studies that focused on animal imaging were mostly conducted at high magnetic fields, such as 7 and 9.4 T. In order to facilitate the potential clinical transition of the developed CD-based contrast agents, the accurate measurement of relaxivities and comparisons to clinical analogs should be performed at 1.5 and 3 T, which are currently used for clinical MRI. During the last eight years, CDs drew attention from researchers working with hyperpolarized MRI. Although only a few studies were conducted, the obtained results demonstrated the high potential of CD macromolecules to become a basis for further development of hyperpolarized probes. Recently, Hane et al. demonstrated that γ-CD-based pseudorotaxanes can be a valuable platform for developing the molecular imaging probes that utilizes the HyperCEST effect [95]. The alkyne chains have a high affinity to the γ-CD cavity and can be easily designed to serve as a high-affinity probe that has a selective binding to the disease site. Functionalizing the alkyne chain should be done carefully such that there is no effect on the interaction with hyperpolarized 129 Xe. The HyperCEST depletion observed was only around 50% indicating partial depolarization of hyperpolarized 129 Xe encapsulated by γ-CD-based pseudorotaxanes. An accurate study for radiofrequency saturation pulses is required to maximize the HyperCEST depletion and to translate γ-CD-based pseudorotaxanes to in vivo imaging applications. Although the demonstrated results of hyperpolarized MRI molecular imaging probes look promising, further in vivo imaging studies are needed, as well as biodistribution and toxicity evaluations of the proposed biosensors. Following the success of the CD-based MRI contrast agents, several attempts have been made to use CDs as a contrast agent for US imaging [44,96]. The developed US CD-based contrast agents caused significant acoustic impedance difference between the tissue and the contrast agents. Despite successful in vitro demonstration of this proof-of-principle [44], there was no further development of the CD-based contrast agent for US imaging purposes during the last five years. On the contrary, CD-coated NPs became of interest for the PAI. It was found that coating of UCNPs with α-CD cause photoacoustic signal enhancement [49] and, therefore, can serve as efficient PAI contrast. The most recent achievement in the field demonstrated PAI detection of prosthetic joint infection using β-CD-conjugated indocyanine green in mice [48]. All studies with CD-based PAI tracers were conducted in small animals. Since the PAI tracers required irradiation with infrared light to emit ultrasound, further studies should move to the larger animals in order to evaluate penetration depth of the excitation light and to estimate the performance of PAI contrast agents prior to clinical translation. Furthermore, the development of larger pallet of PAI contrast dedicated for specific diseases would be beneficial to facilitate the future translation into the clinic.
The vast majority of the radiolabeled CDs derivatives were used for PET and SPECT imaging of the drug delivery and biodistribution in vivo. To be suitable for PET, multiple CD-based tracers containing 64 Cu, 68 Ga, and 18 F were developed. For SPECT imaging purposes, CD macrocycles were radiolabeled with either 99m Tc or 125 I. The further widening of the radiolabeled CD derivatives might become a useful tool for pharmaceutics and drug development.
The most recent CD-based NPs were studied as contrast agents for CT. To be able to produce a sufficient contrast, the CD-based NPs must contain atoms with a high atomic number. The heavy atoms absorb X-rays with higher efficiency, increasing the X-ray attenuation coefficient of the tissue in which the contrast agent is present [107][108][109]. With modern advances in X-ray detection allowing lower dose image acquisition [110][111][112][113], the utilization of the novel CD-based NPs containing heavy elements will be highly beneficial for accurate anatomical imaging purposes. In addition, the novel dual energy CT approach [114,115] will benefit even more from the implementation of the novel CD-based NPs. Implementation and further development of the demonstrated NPs with substantially higher X-ray attenuation compared to currently available iodine and barium contrasts could potentially allow a superior improvement of CNR of the dual energy CT image. CT contrast of CD-based NPs was initially observed from contrast agents developed for multimodal imaging [50,53]. Only during the last three years has the research in synthesis of dedicated CT CD-based contrast agents been conducted extensively. Currently, the vast majority of the CD-based CT contrast agents were developed specifically for cancer imaging, whereas only one agent for angiography was developed. Therefore, it might be potentially useful to develop CD-based contrast agents suitable for different types of clinical CT imaging. In addition, the potential CD-based CT contrast agents must undergo an accurate toxicity study prior to further translation to in vivo imaging. Furthermore, special attention should be given to the investigation of extraction pathways of the contrast agents from the living organism during in vivo studies.
One of the potential approaches for further advances in the field of CD-based contrast agents is the development of a contrast agent that can serve for dual imaging modalities such as PET/MRI and PET/CT. Usually, the PET-active component of the tracer is small, and therefore, the development of the PET/MRI and PET/CT contrast agents could be built around radiolabeling of the existing CD-based agents for MRI and CT imaging modalities, respectively.
Overall, CDs are of high interest in the medical imaging field and are currently a very promising basis for developing various contrast agents. The successful clinical translation of CD-based contrast agents for proton MRI can help significantly improve the quality of clinical MRI scans. Further development of functionalized CD-based imaging agents for MRI has the potential to make molecular imaging using clinical proton MRI possible. Despite the enormous development level of CD-based contrast agents for conventional proton MRI, other imaging modalities started utilizing CD-based contrast agents recently and further developments and investigations are needed prior to successful clinical translation. Nevertheless, CD-based contrast agents demonstrated exceptional performance in the areas of CT, PET, and PAI.