Recent Advances in One-Pot Modular Synthesis of 2-Quinolones

It is known that 2-quinolones are broadly applicable chemical structures in medicinal and agrochemical research as well as various functional materials. A number of current publications about their synthesis and their applications emphasize the importance of these small molecules. The early synthetic chemistry originated from the same principle of the classical Friedländer and Knorr procedures for the preparation of quinolines. The analogous processes were developed by applying new synthetic tools such as novel catalysts, the microwave irradiation method, etc., whereas recent innovations in new bond forming reactions have allowed for novel strategies to construct the core structures of 2-quinolones beyond the bond disconnections based on two classical reactions. Over the last few decades, some reviews on structure-based, catalyst-based, and bioactivity-based studies have been released. In this focused review, we extensively surveyed recent examples of one-pot reactions, particularly in view of modular approaches. Thus, the contents are categorized as three major sections (two-, three-, and four-component reactions) according to the number of reagents that ultimately compose atoms of the core structures of 2-quinolones. The collected synthetic methods are discussed from the perspectives of strategy, efficiency, selectivity, and reaction mechanism.

Understanding the structural character of 2-quinolones is critical to the design of drug-like molecules with optimal binding affinity. The actual structure is deeply related to the external factors that influence tautomeric forms of 2-quinolones during the biological process [50][51][52][53]. Accordingly, studies on the tautomerism of 2-quinolone ( Figure 2) have been of great interest in a similar manner as 4-quinolones [54][55][56][57][58][59]. The tautomerism has been studied by spectroscopic and computational methods [60][61][62][63][64]. The dominant presence of 2-quinolones over 2-hydroxyquinoline in nonaqueous phase or in solid state has been explained by the hydrogen-bonded dimeric stabilization of 2quinolones [65]. In addition to hydrogen bonding, the equilibrium between the two forms appears to be determined by factors such as solvent polarity [66], steric hindrance, and the electronic properties of substituents [67]. Studies on the equilibrium of six-membered N-heterocycles indicates that the reduced aromaticity may contribute to the tautomerization of 2-quinolones as well [68]. The development of synthetic methodologies for 2-quinolones has a long history. Conventional approaches represent two types of reaction: Friedänder-type reaction and Knorr-type reaction ( Figure 3A). Holding high practicality and scalability for setting a good platform for further functionalization, the two methods have been used in a complementary fashion. The Friedänder-type reaction is the base-assisted intermolecular reaction between 2-aminoacylarenes and dialkyl malonates [69,70]. Considering bond disconnections, it needs a C-C double bond formation via Knoevenagel reaction and a C-N amide bond formation via nucleophilic substitution of amines to the esters. In the acid-assisted Knorr-type reaction [71][72][73][74][75][76][77], electrophilic aromatic substitution followed by dehydration forms Csp2-Csp2 bonds. While the two methods have inspired the development of numerous procedures based on the same bond disconnection, recent studies show remarkable advances in the synthesis of this small molecule by varying strategies that originate from new standpoints of bond disconnections ( Figure 3B).
In 2006, Cacchi and coworkers reported the synthesis of 4-aryl-2-quinolones via intermediates 8 under Pd(OAc)2 and potassium acetate in N,N-dimethylformamide (DMF). The Heck-type coupling reaction between 6 and 7 smoothly proceeded to form intermediate 8, which was isolated to identify the structure. Intermediate 8 underwent aminocyclization to provide the desired products 9 in 30-80% yields [85]. The Heck-type coupling reaction between 6 and 7 smoothly proceeded to form Scheme 1. Synthesis of 2-quinolones by Pd-catalyzed reaction of 2-iodoanilines.
In 2006, Cacchi and coworkers reported the synthesis of 4-aryl-2-quinolones via intermediates 8 under Pd(OAc) 2 and potassium acetate in N,N-dimethylformamide (DMF). The Heck-type coupling reaction between 6 and 7 smoothly proceeded to form intermediate 8, which was isolated to identify the structure. Intermediate 8 underwent aminocyclization to provide the desired products 9 in 30-80% yields [85]. The Heck-type coupling reaction between 6 and 7 smoothly proceeded to form intermediates 8; one of the intermediates 8 was isolated to identify the structure. The reaction tolerated electron-rich and electron-poor substituents in both methyl-β-(2-acetamidoaryl)acrylates 6 and aryl iodides 7 (Scheme 2). Scheme 1. Synthesis of 2-quinolones by Pd-catalyzed reaction of 2-iodoanilines.
In 2007, the same authors reported a phosphine ligand-free palladium-catalyzed domino Heck-cyclization reaction. As a complement to the previous work [85], acrylamides 10 instead of acrylic esters 6 were successfully utilized for the synthesis of 4-aryl-2-quinolones [86]. β-(2-Bromoaryl)acrylamides 10 reacted with aryl iodides 11 with electron-poor or -rich groups and the desired products 13 were obtained in 20-80% yields in the presence of molten Bu 4 NOAc and Bu 4 NBr combination (Scheme 3). A palladium-catalyzed pseudo-domino sequence was involved through Heck-type coupling followed by the Buchwald-Hartwig amination. It is noteworthy that phosphine ligand-free palladium-catalyzed domino Heck cyclization reaction is one of the few cases [87]. The reactive palladium nanoparticles stabilized by the molten Bu 4 NOAc and Bu 4 NBr salts mixture were tentatively suggested as the catalyst with high surface area.
In 2007, Cho and Kim reported that the reaction between 2-iodoaniline and dialkyl itaconates furnished 2-quinolones possessing carboalkoxymethyl substituents at the C-3 position. The optimized conditions were found to involve Pd(OAc) 2 , PPh 3 , and NaOAc as a base at 100 • C for 20 h. Various dialkyl itaconates were investigated and the desired products were obtained in 67-76% yields [88]. Meanwhile, the reaction of α, β-unsaturated ketones possessing benzyl substituent at α-position, such as 3-methylene-4-phenyl-2-butanone, afforded a 2,3-dialkylsubstituted quinoline, not a quinolone. The reaction mechanism involves the oxidative addition of iodoaniline 14 to a catalytically active Pd(0) complex. Then, the arylpalladium species I adds across the alkene 15 to give alkylpalladium species II, followed by β-hydrogen elimination to afford the Heck products III and/or IV, which further cyclize to produce 2-quinolones 16 (Scheme 4). desired products 13 were obtained in 20-80% yields in the presence of molten Bu4NOAc and Bu4NBr combination (Scheme 3). A palladium-catalyzed pseudo-domino sequence was involved through Heck-type coupling followed by the Buchwald-Hartwig amination. It is noteworthy that phosphine ligand-free palladium-catalyzed domino Heck cyclization reaction is one of the few cases [87]. The reactive palladium nanoparticles stabilized by the molten Bu4NOAc and Bu4NBr salts mixture were tentatively suggested as the catalyst with high surface area. In 2007, Cho and Kim reported that the reaction between 2-iodoaniline and dialkyl itaconates furnished 2-quinolones possessing carboalkoxymethyl substituents at the C-3 position. The optimized conditions were found to involve Pd(OAc)2, PPh3, and NaOAc as a base at 100 °C for 20 h. Various dialkyl itaconates were investigated and the desired products were obtained in 67-76% yields [88]. Meanwhile, the reaction of α, β-unsaturated ketones possessing benzyl substituent at αposition, such as 3-methylene-4-phenyl-2-butanone, afforded a 2,3-dialkylsubstituted quinoline, not a quinolone. The reaction mechanism involves the oxidative addition of iodoaniline 14 to a catalytically active Pd(0) complex. Then, the arylpalladium species I adds across the alkene 15 to give alkylpalladium species II, followed by β-hydrogen elimination to afford the Heck products III and/or IV, which further cyclize to produce 2-quinolones 16 (Scheme 4).
In 2011, a unified strategy was reported by Borhade and Waghmode via two sequential Heck coupling reactions: (1) the first Heck-type Pd-NiFe2O4-catalyzed coupling reaction of o-iodoaniline 17 and acrylic acid 18 to afford the 2-aminocinnamic acid 19, (2) another Heck coupling reaction of 19 and haloarenes 20 in the same pot to give 21 [89]. It turned out that the use of heterogeneous Pd-NiFe2O4 is a significant advantage in the separation process because it can be easily removed by applying an external magnetic field. Better yields with the aryl iodides were achieved compared with bromoarenes. The preparation of pharmaceutically important 3-alkenyl-4-aryl-2-quinolones by using the key intermediate 21 was demonstrated in this report (Scheme 5). Scheme 5. Pd/NiFe2O4-catalyzed one-pot synthesis of 4-aryl-2-quinolone derivatives. Scheme 5. Pd/NiFe 2 O 4 -catalyzed one-pot synthesis of 4-aryl-2-quinolone derivatives.
In 2014, Liu and Wang developed a novel method for the synthesis of 4-alkylthio-substituted 2-quinolones 36; C-S activation/aryne insertion/intramolecular C-N bond coupling reaction were three consecutive reactions under Pd-catalysis. The method used 2-carbamoyl ketene dithioacetals 34 and in situ generated arynes 35 (Scheme 8) [92]. Aryl-and alkyl substituents at the α-position of the ketene dithioacetals were tolerated; however, a ketene with a primary amide proved to be less efficient. The arynes with both electron-donating and -withdrawing groups were compatible in the optimized conditions to give 2-quinolones in moderate yields (41-51%). The mechanism of the annulation was proposed to be initiated by the cyclopalladation of an in situ generated aryne 35 that forms a palladacycle I. Then, insertion of I into the C-S bond of 34 occurs to afford the intermediate III, presumably via the species II. The subsequent desulfenylation followed by reductive elimination of IV with primary amides 23 gave 3-aryl-2-quinolones in moderate to excellent yields [90]. In the reaction, the C-C double bond was constructed by Knoevenagel condensation and the C-N cross-coupling was enabled by the Buchwald-Hartwig amination. The electronically varied substituents at the benzene ring were all compatible (Scheme 6a). Besides o-halo-substituted benzaldehydes, the substrates including 2bromoacetophenone, 2-bromobenzophenone, methyl 2-bromobenzoate, methyl 2-bromo-5phenylbenzoate, and 2-bromobenzonitrile worked well in the given reaction conditions, affording the corresponding 3,4-disubstituted 2-quinolones (Scheme 6b). In addition to primary amides, secondary amides, e.g., N-methyl-2-aryl acetamides 28, were also suitable substrates in the reaction to give Nmethyl-2-quinolones 29 (Scheme 6c). The broad reaction scope appears to be practically useful. Scheme 6. Synthesis of 2-quinolones by tandem Pd(0)-catalyzed amination/aldol condensation of aryl halides with acetamides.
Another Pd-catalyzed cascade reaction of Buchwald-type amidation and Knoevenagel reaction was reported in 2015. In the reaction, the substrates such as N-alkoxyamides 30 with benzaldehydes 31 or 2-bromo-methyl benzoates 32 were employed to yield the desired N-alkoxyquinolones in moderate to excellent yields, 63-96% (Scheme 7) [91]. With the optimized conditions (Pd2(dba)3, Xantphos, and Cs2CO3 in toluene), various N-alkoxyquinolin-2(1H)-ones 33 with a broad range of functional groups were successfully synthesized. In 2014, Liu and Wang developed a novel method for the synthesis of 4-alkylthio-substituted 2quinolones 36; C-S activation/aryne insertion/intramolecular C-N bond coupling reaction were three consecutive reactions under Pd-catalysis. The method used 2-carbamoyl ketene dithioacetals 34 and in situ generated arynes 35 (Scheme 8) [92]. Aryl-and alkyl substituents at the α-position of the ketene dithioacetals were tolerated; however, a ketene with a primary amide proved to be less efficient. The arynes with both electron-donating and -withdrawing groups were compatible in the optimized conditions to give 2-quinolones in moderate yields (41-51%). The mechanism of the annulation was In 2015, Xu and coworkers disclosed a new methodology for the construction of N-methoxy 2quinolones 39 by palladium-catalyzed N-H activation/Heck reaction [93]. The reaction coupled acryl amides 37 to arynes 38, which was enabled by a catalytic Pd(OAc)2 and hyperstoichiometric use of Cu(OAc)2 and CsF in mixed solvents of dioxane and DMSO at 80 °C (Scheme 9). The acrylamides with electron-rich or -deficient aryl substituents at the α-position afforded 39 in good yields. However, the reactions using cinnamides or cryotonyl amides instead of acryl amides were not successful, thus indicating the importance of strain release. The control experiments and KIE (kinetic isotope effect) measurement showed that aminopalladation of 37 to benzyne is the key step of the catalytic cycle. Scheme 8. Synthesis of 2-quinolinones by Pd-catalyzed C-S activation/aryne insertion/intramolecular C-N coupling reaction.
In 2015, Xu and coworkers disclosed a new methodology for the construction of N-methoxy 2-quinolones 39 by palladium-catalyzed N-H activation/Heck reaction [93]. The reaction coupled acryl amides 37 to arynes 38, which was enabled by a catalytic Pd(OAc) 2 and hyperstoichiometric use of Cu(OAc) 2 and CsF in mixed solvents of dioxane and DMSO at 80 • C (Scheme 9). The acrylamides with electron-rich or -deficient aryl substituents at the α-position afforded 39 in good yields. However, the reactions using cinnamides or cryotonyl amides instead of acryl amides were not successful, thus indicating the importance of strain release. The control experiments and KIE (kinetic isotope effect) measurement showed that aminopalladation of 37 to benzyne is the key step of the catalytic cycle. In 2015, Xu and coworkers disclosed a new methodology for the construction of N-methoxy 2quinolones 39 by palladium-catalyzed N-H activation/Heck reaction [93]. The reaction coupled acryl amides 37 to arynes 38, which was enabled by a catalytic Pd(OAc)2 and hyperstoichiometric use of Cu(OAc)2 and CsF in mixed solvents of dioxane and DMSO at 80 °C (Scheme 9). The acrylamides with electron-rich or -deficient aryl substituents at the α-position afforded 39 in good yields. However, the reactions using cinnamides or cryotonyl amides instead of acryl amides were not successful, thus indicating the importance of strain release. The control experiments and KIE (kinetic isotope effect) measurement showed that aminopalladation of 37 to benzyne is the key step of the catalytic cycle. Scheme 9. Synthesis of quinolones by Pd-catalyzed oxidative annulation between acrylamides and aryne precursors. Liu and coworkers have reported a Pd-catalyzed cascade reaction of C-H bond activation/C-C bond formation/intramolecular cyclization by employing substituted anilines 40 and acrylates 41 [94]. During reaction optimizations, an in situ generated amide in the presence of Ac 2 O was the key to initiating arene C-H activation. The hydrolysis of the acetyl group was promoted by the addition of TsOH·H 2 O (Scheme 10). The anilines 40 possessing electron-donating and -withdrawing groups worked smoothly to give the corresponding products 42 in moderate to excellent yields. However, the reactions using anilines possessing trifluoromethyl-, nitro-, or carbonyl groups at the para position were unsuccessful. Cinnamates 41 bearing electron-rich groups on R 2 (aryl groups) showed higher reactivity than those with electron-deficient groups.
Liu and coworkers have reported a Pd-catalyzed cascade reaction of C-H bond activation/C-C bond formation/intramolecular cyclization by employing substituted anilines 40 and acrylates 41 [94]. During reaction optimizations, an in situ generated amide in the presence of Ac2O was the key to initiating arene C-H activation. The hydrolysis of the acetyl group was promoted by the addition of TsOH•H2O (Scheme 10). The anilines 40 possessing electron-donating and -withdrawing groups worked smoothly to give the corresponding products 42 in moderate to excellent yields. However, the reactions using anilines possessing trifluoromethyl-, nitro-, or carbonyl groups at the para position were unsuccessful. Cinnamates 41 bearing electron-rich groups on R 2 (aryl groups) showed higher reactivity than those with electron-deficient groups. Scheme 10. Synthesis of quinolin-2(1H)-ones by a Pd-catalyzed C-H activation/C-C bond formation/intramolecular cyclization reaction.
Quinolinone III was an important intermediate in the preparation of the potent, orally active inhibitor of farnesyl protein transferase, Tipifarnib. The developed method provided a more stepeconomical way to prepare it compared to the previous synthetic routes involving multi-step synthesis.
In 2017, Hu and his coworkers disclosed a Pd(II)-catalyzed intermolecular Heck reaction/intramolecular C-H amidation reaction to prepare 4-aryl-2-quinolones 45 (Scheme 11). The reaction of cinnamides 43 containing 2-(pyridin-2-yl)ethanamine with aryl iodides 44 was carried out in the presence of Pd(OAc)2, PPh3, K2CO3, and copper/silver oxidants in toluene. The aryl iodides 44 with electron-donating groups such as methoxy and methyl groups efficiently worked to give the corresponding products in moderate yields. However, those with electron-withdrawing groups such as -F, -NO2, -CN, -CF3, and -CO2Me were less effective [95]. A proposed reaction mechanism is depicted in Scheme 11. The first step begins with the Heck reaction of I and aryl iodides and then an intramolecular C-H amidation reaction occurs to provide 45. The C-H amidation was promoted by a pendant group of 2-(pyridin-2-yl)ethanamine which likely directs the Pd(II) catalyst towards the proximal C(sp 2 )-H bond to generate the palladacycle intermediate III.
Quinolinone III was an important intermediate in the preparation of the potent, orally active inhibitor of farnesyl protein transferase, Tipifarnib. The developed method provided a more step-economical way to prepare it compared to the previous synthetic routes involving multi-step synthesis.
In 2017, Hu and his coworkers disclosed a Pd(II)-catalyzed intermolecular Heck reaction/intramolecular C-H amidation reaction to prepare 4-aryl-2-quinolones 45 (Scheme 11). The reaction of cinnamides 43 containing 2-(pyridin-2-yl)ethanamine with aryl iodides 44 was carried out in the presence of Pd(OAc) 2 , PPh 3 , K 2 CO 3 , and copper/silver oxidants in toluene. The aryl iodides 44 with electron-donating groups such as methoxy and methyl groups efficiently worked to give the corresponding products in moderate yields. However, those with electron-withdrawing groups such as -F, -NO 2 , -CN, -CF 3 , and -CO 2 Me were less effective [95]. A proposed reaction mechanism is depicted in Scheme 11. The first step begins with the Heck reaction of I and aryl iodides and then an intramolecular C-H amidation reaction occurs to provide 45.

Other Transition-Metal-Catalyzed Reactions Using Cu, Ag, Rh, Ir, and Ni
Decades of research have demonstrated that conventional palladium catalysts can be replaced by the first, second, and third row transition metals such as copper, silver, rhodium, and iridium for the preparation of 2-quinolones.
The copper-ethylene diamine combination has been found to be an efficient catalytic system for the synthesis of 2-quinolinones 48 via cascade reactions of substituted 2-halobenzocarbonyls 46 with 2-arylacetamides 47 (Scheme 12). The use of ethylenediamine ligand has broadened the substrate scope. The reaction did not efficiently proceed in polar solvent such as N,N-dimethylformamide, whereas use of toluene ensured high conversion [96]. Although the protocol worked efficiently for less reactive aryl chloride, use of 2-iodobenzocarbonyls provided 2-quinolineone products 48 in better yields than 2-bromo-or 2-chlorobenzocarboyls in the given reaction conditions. Moreover, the reaction of arylacetamide 47 with electron-donating R 4 substituents provided the desired products in higher yields than the reaction with an electron-withdrawing group (NO2).

Other Transition-Metal-Catalyzed Reactions Using Cu, Ag, Rh, Ir, and Ni
Decades of research have demonstrated that conventional palladium catalysts can be replaced by the first, second, and third row transition metals such as copper, silver, rhodium, and iridium for the preparation of 2-quinolones.
The copper-ethylene diamine combination has been found to be an efficient catalytic system for the synthesis of 2-quinolinones 48 via cascade reactions of substituted 2-halobenzocarbonyls 46 with 2-arylacetamides 47 (Scheme 12). The use of ethylenediamine ligand has broadened the substrate scope. The reaction did not efficiently proceed in polar solvent such as N,N-dimethylformamide, whereas use of toluene ensured high conversion [96]. Although the protocol worked efficiently for less reactive aryl chloride, use of 2-iodobenzocarbonyls provided 2-quinolineone products 48 in better yields than 2-bromo-or 2-chlorobenzocarboyls in the given reaction conditions. Moreover, the reaction of arylacetamide 47 with electron-donating R 4 substituents provided the desired products in higher yields than the reaction with an electron-withdrawing group (NO 2 ).

Other Transition-Metal-Catalyzed Reactions Using Cu, Ag, Rh, Ir, and Ni
Decades of research have demonstrated that conventional palladium catalysts can be replaced by the first, second, and third row transition metals such as copper, silver, rhodium, and iridium for the preparation of 2-quinolones.
The copper-ethylene diamine combination has been found to be an efficient catalytic system for the synthesis of 2-quinolinones 48 via cascade reactions of substituted 2-halobenzocarbonyls 46 with 2-arylacetamides 47 (Scheme 12). The use of ethylenediamine ligand has broadened the substrate scope. The reaction did not efficiently proceed in polar solvent such as N,N-dimethylformamide, whereas use of toluene ensured high conversion [96]. Although the protocol worked efficiently for less reactive aryl chloride, use of 2-iodobenzocarbonyls provided 2-quinolineone products 48 in better yields than 2-bromo-or 2-chlorobenzocarboyls in the given reaction conditions. Moreover, the reaction of arylacetamide 47 with electron-donating R 4 substituents provided the desired products in higher yields than the reaction with an electron-withdrawing group (NO2). A contribution with Cu-catalyzed 2-quinolone synthesis was reported by Lim and coworkers in 2018. In their study, dendritic copper powder was discovered to promote both Knoevenagel condensation and C-N bond formation, and 3-amido-2-quinolones 51 were synthesized by the reaction with 2-bromobenzaldehydes 49 and malonamides 50 in one pot [97]. The key factor of the method was the use of an amine base that did not hydrolyze the amide. Besides the malonamides, other active methylenes containing p-fluorophenyl and bulky, adamantly substituted amides were found to be smoothly annulated to give the corresponding 3-substituted 2-quinolones (Scheme 13). During mechanistic studies, dendritic copper powder was proven to play a dual role as enabler of C-N bond formation and promoter of Knoevenagel condensation. A contribution with Cu-catalyzed 2-quinolone synthesis was reported by Lim and coworkers in 2018. In their study, dendritic copper powder was discovered to promote both Knoevenagel condensation and C-N bond formation, and 3-amido-2-quinolones 51 were synthesized by the reaction with 2-bromobenzaldehydes 49 and malonamides 50 in one pot [97]. The key factor of the method was the use of an amine base that did not hydrolyze the amide. Besides the malonamides, other active methylenes containing p-fluorophenyl and bulky, adamantly substituted amides were found to be smoothly annulated to give the corresponding 3-substituted 2-quinolones (Scheme 13). During mechanistic studies, dendritic copper powder was proven to play a dual role as enabler of C-N bond formation and promoter of Knoevenagel condensation. Mai and coworkers have achieved the practical synthesis of 3-acyl-4-aryl-2-quinolones 54 using AgNO3 and K2S2O8 as a radical generator [98]. The reaction with higher loading (4 equiv) of oxidant K2S2O8 was better than that with lower loading (1.0-2.5 equiv) and the product was obtained in an 83% yield. A wide range of both electron-withdrawing and -donating substituents such as o-Cl, p-Br, p-OMe, and 3, 4-diOMe on the phenyl ring of cinnamic acid 52 were compatible. In addition, 2-oxo-2-phenyl acetic acids 53 containing electron-withdrawing and -donating groups proceeded smoothly to produce 54 in good yields. The proposed reaction mechanism of this radical tandem cyclization involves acyl radical trapping, generated from carboxylate 53, by the α-position of the N-methyl-Nphenyl cinnamamides 52 followed by intramolecular cyclization to give an intermediate III. The aromatizative dehydrogenation likely results in the formation of the final products 54 (Scheme 14).
Duane and Zhang reported copper-mediated one-pot cascade cyclization of acrylamides and arynes. A catalytic system of Cu(OAc)2 and O2 in DMA/MeCN was discovered for the efficient preparation of 4-aryl-2-quinolones 58. It is notable that Cu(OAc)2 showed higher reactivity than Pd(OAc)2 [99]. A wide range of substituents on the α-position of monosubstituted acrylamides, e.g., aryl, alkyl, and cycloalkyl, were well tolerated to provide to 3-disubstituted 2-quinolones. However, only 39% yield was obtained with α-fluorinated acrylamide. In addition, α,β-disubstituted acrylamides could be utilized to generate 3,4-disubstituted 2-quinolinones in moderate yields. Through some control experiments, including an intermolecular KIE (value = 5.1) and radical trapping experiment, the single-electron transfer (SET) mechanism could be excluded. On the basis of experimental and computational studies, a plausible mechanism was proposed. The organometallic alkenyl C-H bond activation/addition to arynes via a Cu(III) intermediate 57, presumably through the ring opening of the four-membered ring I, affords the expected products 58 with a new C-N bond after reductive elimination (Scheme 15). Mai and coworkers have achieved the practical synthesis of 3-acyl-4-aryl-2-quinolones 54 using AgNO 3 and K 2 S 2 O 8 as a radical generator [98]. The reaction with higher loading (4 equiv) of oxidant K 2 S 2 O 8 was better than that with lower loading (1.0-2.5 equiv) and the product was obtained in an 83% yield. A wide range of both electron-withdrawing and -donating substituents such as o-Cl, p-Br, p-OMe, and 3, 4-diOMe on the phenyl ring of cinnamic acid 52 were compatible. In addition, 2-oxo-2-phenyl acetic acids 53 containing electron-withdrawing and -donating groups proceeded smoothly to produce 54 in good yields. The proposed reaction mechanism of this radical tandem cyclization involves acyl radical trapping, generated from carboxylate 53, by the α-position of the N-methyl-N-phenyl cinnamamides 52 followed by intramolecular cyclization to give an intermediate III. The aromatizative dehydrogenation likely results in the formation of the final products 54 (Scheme 14).
Duane and Zhang reported copper-mediated one-pot cascade cyclization of acrylamides and arynes. A catalytic system of Cu(OAc) 2 and O 2 in DMA/MeCN was discovered for the efficient preparation of 4-aryl-2-quinolones 58. It is notable that Cu(OAc) 2 showed higher reactivity than Pd(OAc) 2 [99]. A wide range of substituents on the α-position of monosubstituted acrylamides, e.g., aryl, alkyl, and cycloalkyl, were well tolerated to provide to 3-disubstituted 2-quinolones. However, only 39% yield was obtained with α-fluorinated acrylamide. In addition, α,β-disubstituted acrylamides could be utilized to generate 3,4-disubstituted 2-quinolinones in moderate yields. Through some control experiments, including an intermolecular KIE (value = 5.1) and radical trapping experiment, the single-electron transfer (SET) mechanism could be excluded. On the basis of experimental and computational studies, a plausible mechanism was proposed. The organometallic alkenyl C-H bond activation/addition to arynes via a Cu ( In 2018, Kuhakarn and coworkers disclosed a novel annulation technique using o-alkynylisonitrile and H 2 O in the presence of a catalytic amount of silver nitrate [100]. While not clearly demonstrated, the proposed mechanism suggests that alkyne activation by a silver cation would be followed by nucleophilic addition of the carbanion to the activated alkynes; the carbanion seems to be generated by H 2 O addition to isocyanate (Scheme 16). Besides silver (I) salts, other metal complexes such as Cu(I), Cu(II), Zn(II), and Fe(III) as well as tosylic acid worked in the designed reaction system. A variety of 3-substituted 2-quinolone derivatives were successfully synthesized by the method.
Molecules 2020, 25, x FOR PEER REVIEW 15 of 42 In 2018, Kuhakarn and coworkers disclosed a novel annulation technique using oalkynylisonitrile and H2O in the presence of a catalytic amount of silver nitrate [100]. While not clearly demonstrated, the proposed mechanism suggests that alkyne activation by a silver cation would be followed by nucleophilic addition of the carbanion to the activated alkynes; the carbanion seems to be generated by H2O addition to isocyanate (Scheme 16). Besides silver (I) salts, other metal complexes such as Cu(I), Cu(II), Zn(II), and Fe(III) as well as tosylic acid worked in the designed reaction system. A variety of 3-substituted 2-quinolone derivatives were successfully synthesized by the method. In 2014, Jeganmohan's research group reported ruthenium-catalyzed synthesis of 2quinolinones with anilides 61 and unsaturated hydrocarbons such as acrylates (Route I) or propiolates (Route II) [101]. The alkyl acrylates were first tested for the synthesis of 2-quinolones, and the reaction with methyl acrylate afforded the corresponding products in a better yield than those with ethyl or n-butyl acrylates. Anilides with various substituents such as OMe, F, or COMe were successfully reacted with methyl acrylates, providing the substituted 2-quinolones 62 in good yields (Route I). The substituted propiolates were also suitable substrates in the designed transformation. In this way, 4-substituted-2-quinolones 63 were obtained. It is interesting to see that each substrate optionally required a different type of acid source for better conversion, e.g., pivalic acid or acetic acid. The reaction scope was quite general; a variety of 2-quinolones with electron-donating,withdrawing, or -neutral substituents were prepared in good to excellent yields (Scheme 17).
The alkyl acrylates were first tested for the synthesis of 2-quinolones, and the reaction with methyl acrylate afforded the corresponding products in a better yield than those with ethyl or n-butyl acrylates. Anilides with various substituents such as OMe, F, or COMe were successfully reacted with methyl acrylates, providing the substituted 2-quinolones 62 in good yields (Route I). The substituted propiolates were also suitable substrates in the designed transformation. In this way, 4-substituted-2-quinolones 63 were obtained. It is interesting to see that each substrate optionally required a different type of acid source for better conversion, e.g., pivalic acid or acetic acid. The reaction scope was quite general; a variety of 2-quinolones with electron-donating, -withdrawing, or -neutral substituents were prepared in good to excellent yields (Scheme 17).
Molecules 2020, 25, x FOR PEER REVIEW 15 of 42 In 2018, Kuhakarn and coworkers disclosed a novel annulation technique using oalkynylisonitrile and H2O in the presence of a catalytic amount of silver nitrate [100]. While not clearly demonstrated, the proposed mechanism suggests that alkyne activation by a silver cation would be followed by nucleophilic addition of the carbanion to the activated alkynes; the carbanion seems to be generated by H2O addition to isocyanate (Scheme 16). Besides silver (I) salts, other metal complexes such as Cu(I), Cu(II), Zn(II), and Fe(III) as well as tosylic acid worked in the designed reaction system. A variety of 3-substituted 2-quinolone derivatives were successfully synthesized by the method. In 2014, Jeganmohan's research group reported ruthenium-catalyzed synthesis of 2quinolinones with anilides 61 and unsaturated hydrocarbons such as acrylates (Route I) or propiolates (Route II) [101]. The alkyl acrylates were first tested for the synthesis of 2-quinolones, and the reaction with methyl acrylate afforded the corresponding products in a better yield than those with ethyl or n-butyl acrylates. Anilides with various substituents such as OMe, F, or COMe were successfully reacted with methyl acrylates, providing the substituted 2-quinolones 62 in good yields (Route I). The substituted propiolates were also suitable substrates in the designed transformation. In this way, 4-substituted-2-quinolones 63 were obtained. It is interesting to see that each substrate optionally required a different type of acid source for better conversion, e.g., pivalic acid or acetic acid. The reaction scope was quite general; a variety of 2-quinolones with electron-donating,withdrawing, or -neutral substituents were prepared in good to excellent yields (Scheme 17).
In 2019, Zhu reported a Rh-catalyzed, amide-directed, double C-H bond activation strategy to prepare cycloalkaquinolones 66 with arylboronic acid 64 pinacol esters 65 (Scheme 18) [102]. The products were obtained in moderate to excellent yields and showed broad scope and high functional group tolerance. The mechanism firstly involves the formation of the silver-aided, five-membered rhodacycle I and transmetallation with boronic ester followed by reductive elimination, affording the β-C-H arylated product III. Then, the deprotonative coordination of the nitrogen in III to rhodium species triggers the second C-H bond activation, giving seven-membered rhodacycle IV. Finally, 2-quinolone 66 is provided after reductive elimination. The control experiments including H/D exchange and parallel KIE experiments strongly suggest that this reaction is initiated via the concerted metalation deprotonation (CMD) pathway, and the cleavage of the alkenyl C-H bond is probably the rate-determining step. This synthetic method successfully afforded cycloalkaquinolone scaffolds, which are often found in natural products with pharmacological interest. In 2019, Zhu reported a Rh-catalyzed, amide-directed, double C-H bond activation strategy to prepare cycloalkaquinolones 66 with arylboronic acid 64 pinacol esters 65 (Scheme 18) [102]. The products were obtained in moderate to excellent yields and showed broad scope and high functional group tolerance. The mechanism firstly involves the formation of the silver-aided, five-membered rhodacycle I and transmetallation with boronic ester followed by reductive elimination, affording the β-C-H arylated product III. Then, the deprotonative coordination of the nitrogen in III to rhodium species triggers the second C-H bond activation, giving seven-membered rhodacycle IV. Finally, 2quinolone 66 is provided after reductive elimination. The control experiments including H/D exchange and parallel KIE experiments strongly suggest that this reaction is initiated via the concerted metalation deprotonation (CMD) pathway, and the cleavage of the alkenyl C-H bond is probably the rate-determining step. This synthetic method successfully afforded cycloalkaquinolone scaffolds, which are often found in natural products with pharmacological interest. Rong and Dong disclosed a unique two-component reaction between isatins 67 and alkynes 68 under rhodium-based catalytic conditions [103]. The reaction mode through a catalytic decarbonylative coupling reaction was a distinct point of the reaction design. Given the challenge of the selective C-C bond activation over arene C-H bond activation, the selective C-C bond activation was achieved by controlling the orientation of the directing group (Scheme 19). The methyl group at the 3-position of the pyridine ring was the key to ensuring the correct orientation which directs the rhodium complex to the ketone group of the isatins. During the mechanistic studies to prove the pathway by C-C bond activation, a Rh complex II (L = pyridine) was successfully isolated and, interestingly, it was generated at room temperature. However, alkyne insertion to II did not occur below 130 °C. This tentatively suggests that a high reaction temperature is required for either the following alkyne insertion step or reductive elimination step. Rong and Dong disclosed a unique two-component reaction between isatins 67 and alkynes 68 under rhodium-based catalytic conditions [103]. The reaction mode through a catalytic decarbonylative coupling reaction was a distinct point of the reaction design. Given the challenge of the selective C-C bond activation over arene C-H bond activation, the selective C-C bond activation was achieved by controlling the orientation of the directing group (Scheme 19). The methyl group at the 3-position of the pyridine ring was the key to ensuring the correct orientation which directs the rhodium complex to the ketone group of the isatins. During the mechanistic studies to prove the pathway by C-C bond activation, a Rh complex II (L = pyridine) was successfully isolated and, interestingly, it was generated at room temperature. However, alkyne insertion to II did not occur below 130 • C. This tentatively suggests that a high reaction temperature is required for either the following alkyne insertion step or reductive elimination step.  Wang and coworkers have developed a microwave-assisted, Friedländer-type condensation method using o-aminoarylketones and esters with a reactive α-methylene moiety (Scheme 20). Cerium chloride heptahydrate (CeCl 3 ·7H 2 O) was used as the catalyst and this common Lewis acid offered excellent yields, with high selectivity towards to 2-quinolones in only 10 min [104]. The reactions between o-aminoarylketones 70 and keto esters were initially nonselective, giving a mixture of 2-quinolones 72 and quinolines 73 under the copper catalysts (CuSO 4 ·5H 2 O, CuCl, and CuCl 2 ·2H 2 O). However, 2-quinolones 72 were obtained as major compounds after optimization in the presence of 0.2 equiv CeCl 3 ·7H 2 O under microwave irradiation (450 W). Wang and coworkers have developed a microwave-assisted, Friedländer-type condensation method using o-aminoarylketones and esters with a reactive α-methylene moiety (Scheme 20). Cerium chloride heptahydrate (CeCl3•7H2O) was used as the catalyst and this common Lewis acid offered excellent yields, with high selectivity towards to 2-quinolones in only 10 min [104]. The reactions between o-aminoarylketones 70 and keto esters were initially nonselective, giving a mixture of 2-quinolones 72 and quinolines 73 under the copper catalysts (CuSO4•5H2O, CuCl, and CuCl2•2H2O). However, 2-quinolones 72 were obtained as major compounds after optimization in the presence of 0.2 equiv CeCl3•7H2O under microwave irradiation (450 W). In 2010, Tsuji and coworkers reported iridium-catalyzed synthesis of 3,4-disubstitued N-methyl-2-quinolones 76 using various N-methylarylcarbamoyl chlorides 74 and internal alkynes 75. The catalytic conditions employed [IrCl(cod)] 2 (cod = 1,5-cyclooctadiene) and cod as a dummy ligand. The reaction tolerated a wide range of substituents in both coupling partners. For example, symmetrical alkynes were well suited in this annulation, providing the desired products in good yields (62-67%). The selectivity was not practically high in cases of the unsymmetrical alkynes. However, it is notable that unsymmetrical alkynes possessing an ether functionality increased the regioselectivity, presumably by directing the effect of the oxygen atom [105]. A reaction pathway was proposed based on control experiments and literature precedents. Initially, Ir(I) is oxidized to Ir(III) by 74, and I is then converted to five-membered iridacycle II by an arene sp 2 C-H activation step. The formation of the iridacycle II plays a crucial role in suppressing the decarbonylation that can lead to deconstructive pathways. The intermediate II was firmly identified by X-ray crystallography of a phosphine-bound iridium phosphine complex. Then, the insertion of alkynes 75 yields seven-membered iridacycle III or III . The subsequent reductive elimination affords 2-quinolones 76 by regenerating the Ir(I) catalyst (Scheme 21). In 2010, Tsuji and coworkers reported iridium-catalyzed synthesis of 3,4-disubstitued N-methyl-2-quinolones 76 using various N-methylarylcarbamoyl chlorides 74 and internal alkynes 75. The catalytic conditions employed [IrCl(cod)]2 (cod = 1,5-cyclooctadiene) and cod as a dummy ligand. The reaction tolerated a wide range of substituents in both coupling partners. For example, symmetrical alkynes were well suited in this annulation, providing the desired products in good yields (62-67%). The selectivity was not practically high in cases of the unsymmetrical alkynes. However, it is notable that unsymmetrical alkynes possessing an ether functionality increased the regioselectivity, presumably by directing the effect of the oxygen atom [105]. A reaction pathway was proposed based on control experiments and literature precedents. Initially, Ir(I) is oxidized to Ir(III) by 74, and I is then converted to five-membered iridacycle II by an arene sp 2 C-H activation step. The formation of the iridacycle II plays a crucial role in suppressing the decarbonylation that can lead to deconstructive pathways. The intermediate II was firmly identified by X-ray crystallography of a phosphine-bound iridium phosphine complex. Then, the insertion of alkynes 75 yields seven-membered iridacycle III or III'. The subsequent reductive elimination affords 2-quinolones 76 by regenerating the Ir(I) catalyst (Scheme 21). Scheme 21. Ir-catalyzed cascade reaction using carbamoyl chlorides and internal alkynes.
Recently, Ye and coworkers reported a new route to 2-quinolones using arylformamides 77 and alkynes 78 via a Ni-Al cooperative bimetallic catalysis. This novel strategy features nonchelated dual C-H activation, discovery of a novel secondary phosphine oxide (SPO) ligand in bimetallic catalysis, wide substrate scope, and good functional group tolerance [106]. The regioselectivity was highly dependent on substituents of alkynes. For example, the reaction of asymmetric dialkyl acetylenes resulted in poor regioselectivities, while the use of alkylphenyl acetylenes led to good regioselectivities (>10:1). It is suggested that the regioselectivity is determined by the electronic factors of the substituents at both sides of alkynes. In cases using alkylphenyl acetylenes, the phenyl was proximate to the carbonyl groups. This is due to favoring a more stable benzylic nickel species during the second alkylphenyl alkyne insertion to the nickelacycle III. The control experiments indicated that the SPO played the role of a bifunctional ligand to facilitate the reaction by the unique coordination mode, as shown in proposed mechanism in Scheme 22. A plausible mechanism for this process involves formation of the intermediate I via oxidative addition of Ni(0) to the formyl C-H bond. This step is proposed to be likely assisted by the SPO-bound Ni-Al complex, which is followed by Ni-H addition across the alkynes 75. Next, the aryl C-H bond cleavage forms the nickelacycle III. The subsequent alkyne insertion and reductive elimination affords 76. Recently, Ye and coworkers reported a new route to 2-quinolones using arylformamides 77 and alkynes 78 via a Ni-Al cooperative bimetallic catalysis. This novel strategy features nonchelated dual C-H activation, discovery of a novel secondary phosphine oxide (SPO) ligand in bimetallic catalysis, wide substrate scope, and good functional group tolerance [106]. The regioselectivity was highly dependent on substituents of alkynes. For example, the reaction of asymmetric dialkyl acetylenes resulted in poor regioselectivities, while the use of alkylphenyl acetylenes led to good regioselectivities (>10:1). It is suggested that the regioselectivity is determined by the electronic factors of the substituents at both sides of alkynes. In cases using alkylphenyl acetylenes, the phenyl was proximate to the carbonyl groups. This is due to favoring a more stable benzylic nickel species during the second alkylphenyl alkyne insertion to the nickelacycle III. The control experiments indicated that the SPO played the role of a bifunctional ligand to facilitate the reaction by the unique coordination mode, as shown in proposed mechanism in Scheme 22. A plausible mechanism for this process involves formation of the intermediate I via oxidative addition of Ni(0) to the formyl C-H bond. This step is proposed to be likely assisted by the SPO-bound Ni-Al complex, which is followed by Ni-H addition across the alkynes 75. Next, the aryl C-H bond cleavage forms the nickelacycle III. The subsequent alkyne insertion and reductive elimination affords 76.

Transition-Metal-Free Reactions
Although many mechanistically distinct methods via two-component reactions are described above, they require the use of expensive noble metals, often with expensive ligands, and external oxidants when needed to re-initiate the catalytic cycle. Meanwhile, the transition-metal-free approaches to functionalized 2-quinolones via two-component reaction have been widely studied.

Transition-Metal-Free Reactions
Although many mechanistically distinct methods via two-component reactions are described above, they require the use of expensive noble metals, often with expensive ligands, and external oxidants when needed to re-initiate the catalytic cycle. Meanwhile, the transition-metal-free approaches to functionalized 2-quinolones via two-component reaction have been widely studied.
Some representative examples are presented in this section.
A classical Friedländer-type transformation could be accelerated by basic mesoporous catalysts. In 2009, Cejka addressed the use of basic heterogeneous catalysts supporting diethylaminopropyl (DEAP), methylaminopropyl (MAP), and aminopropyl (AP) for the synthesis of 3,4-disubstituted 2-quinolones [107]. The primary amine-supported catalyst (AP) resulted in lower yields than the secondary (MAP) or tertiary amine-supported (DEAP) catalyst. The MAP-based catalyst (20 wt %) showed the highest catalytic activity and complete selectivity toward 2-quinolone 82, as depicted in Scheme 23. However, in this reaction, use of the acidic heterogeneous catalysts including Al-SBA-15 and its cesium salt Cs(Al)-SBA-15 was not efficient, resulting in reversed chemoselectivity.
In 2010, López-Peinado's group reported a zeolite-promoted Friedländer reaction [108]. They developed a protocol using bifunctional zeolites with Lewis and Brønsted acid sites such as H-BEA, H-MFI, H-FAU, and H-MOR. The structure and morphology of the catalysts were characterized with IR spectra, X-ray diffraction (XRD), and scanning electron microscopy (SEM). The catalytic performance was evaluated in the condensation of 2-aminoaryl ketones 80 and ethyl acetoacetate 81 at elevated temperature; the results are summarized in Scheme 24. While the conversion and selectivity were not practically high, it is notable that H-MOR with higher Brønsted acid site concentration gave better selectivity towards 2-quinolone 82. A similar approach using Amberlite Na sr1L as a strongly acidic catalyst was reported by Khan and coworkers [109].
(DEAP), methylaminopropyl (MAP), and aminopropyl (AP) for the synthesis of 3,4-disubstituted 2quinolones [107]. The primary amine-supported catalyst (AP) resulted in lower yields than the secondary (MAP) or tertiary amine-supported (DEAP) catalyst. The MAP-based catalyst (20 wt%) showed the highest catalytic activity and complete selectivity toward 2-quinolone 82, as depicted in Scheme 23. However, in this reaction, use of the acidic heterogeneous catalysts including Al-SBA-15 and its cesium salt Cs(Al)-SBA-15 was not efficient, resulting in reversed chemoselectivity. In 2010, López-Peinado's group reported a zeolite-promoted Friedländer reaction [108]. They developed a protocol using bifunctional zeolites with Lewis and Brønsted acid sites such as H-BEA, H-MFI, H-FAU, and H-MOR. The structure and morphology of the catalysts were characterized with IR spectra, X-ray diffraction (XRD), and scanning electron microscopy (SEM). The catalytic performance was evaluated in the condensation of 2-aminoaryl ketones 80 and ethyl acetoacetate 81 at elevated temperature; the results are summarized in Scheme 24. While the conversion and selectivity were not practically high, it is notable that H-MOR with higher Brønsted acid site concentration gave better selectivity towards 2-quinolone 82. A similar approach using Amberlite Na sr1L as a strongly acidic catalyst was reported by Khan and coworkers [109]. In 2014, Bui reported that the condensation reaction between substituted 2-aminobenzophenones 84 and acid chlorides 85 furnished 2-quinolinones 86 in the presence of a base under microwave irradiation [110]. They found that microwave irradiation is important in terms of reaction efficiency, shorter reaction time, and better conversion. The condensation reactions of simple benzophenones with ethyl malonyl chlorides proceeded smoothly to give 84 in good yields, although not relying on microwave irradiation. However, the reaction either of ketones possessing non-methyl alkyl groups at R 2 or aliphatic acid chlorides required microwave irradiation for successful conversion. A strong base, such as sodium hydride (or sodium bis(trimethylsilyl)amide), was required to ensure annulation for the products 87 bearing alkyl substituents at the 3-position (Scheme 25). Deng and coworkers developed cascade annulation of 2-alkenylanilines in the presence of di-tbutyl carbonate (Boc2O) and a catalytic amount of 4-dimethylaminopyridine (DMAP) [111]. DMAP and Boc2O were used for transforming amines to the corresponding isocyanates I [112], which was converted to 2-hydroxyquinoline III or its tautomer IV by 6π-electrocyclization process. The electronwithdrawing and -donating functional groups in arenes were compatible to provide a series of tert-Scheme 24. Friedländer reaction by bifunctional zeolites as Lewis and Brønsted acid catalyst.
In 2014, Bui reported that the condensation reaction between substituted 2-aminobenzophenones 84 and acid chlorides 85 furnished 2-quinolinones 86 in the presence of a base under microwave irradiation [110]. They found that microwave irradiation is important in terms of reaction efficiency, shorter reaction time, and better conversion. The condensation reactions of simple benzophenones with ethyl malonyl chlorides proceeded smoothly to give 84 in good yields, although not relying on microwave irradiation. However, the reaction either of ketones possessing non-methyl alkyl groups at R 2 or aliphatic acid chlorides required microwave irradiation for successful conversion. A strong base, such as sodium hydride (or sodium bis(trimethylsilyl)amide), was required to ensure annulation for the products 87 bearing alkyl substituents at the 3-position (Scheme 25). In 2010, López-Peinado's group reported a zeolite-promoted Friedländer reaction [108]. They developed a protocol using bifunctional zeolites with Lewis and Brønsted acid sites such as H-BEA, H-MFI, H-FAU, and H-MOR. The structure and morphology of the catalysts were characterized with IR spectra, X-ray diffraction (XRD), and scanning electron microscopy (SEM). The catalytic performance was evaluated in the condensation of 2-aminoaryl ketones 80 and ethyl acetoacetate 81 at elevated temperature; the results are summarized in Scheme 24. While the conversion and selectivity were not practically high, it is notable that H-MOR with higher Brønsted acid site concentration gave better selectivity towards 2-quinolone 82. A similar approach using Amberlite Na sr1L as a strongly acidic catalyst was reported by Khan and coworkers [109]. In 2014, Bui reported that the condensation reaction between substituted 2-aminobenzophenones 84 and acid chlorides 85 furnished 2-quinolinones 86 in the presence of a base under microwave irradiation [110]. They found that microwave irradiation is important in terms of reaction efficiency, shorter reaction time, and better conversion. The condensation reactions of simple benzophenones with ethyl malonyl chlorides proceeded smoothly to give 84 in good yields, although not relying on microwave irradiation. However, the reaction either of ketones possessing non-methyl alkyl groups at R 2 or aliphatic acid chlorides required microwave irradiation for successful conversion. A strong base, such as sodium hydride (or sodium bis(trimethylsilyl)amide), was required to ensure annulation for the products 87 bearing alkyl substituents at the 3-position (Scheme 25). Deng and coworkers developed cascade annulation of 2-alkenylanilines in the presence of di-tbutyl carbonate (Boc2O) and a catalytic amount of 4-dimethylaminopyridine (DMAP) [111]. DMAP and Boc2O were used for transforming amines to the corresponding isocyanates I [112], which was converted to 2-hydroxyquinoline III or its tautomer IV by 6π-electrocyclization process. The electronwithdrawing and -donating functional groups in arenes were compatible to provide a series of tert-Scheme 25. Synthesis of 3,4-disubstituted 2-quinolones by one-pot condensation promoted by microwave irradiation. [111]. DMAP and Boc 2 O were used for transforming amines to the corresponding isocyanates I [112], which was converted to 2-hydroxyquinoline III or its tautomer IV by 6π-electrocyclization process. The electron-withdrawing and -donating functional groups in arenes were compatible to provide a series of tert-butyl quinolin-2-yl carbonates 89 in moderate to good yields. Deprotection of the tert-butyloxycarbonyl (Boc) group under H 2 SO 4 condition could lead to 2-quinolones. A gram-scale reaction (1.56 g of starting material, R 1 = H, R 2 = Ph) was conducted and the desired product was successfully obtained in 92%. Moreover, one of the hepatitis B virus (HBV) inhibitors could be prepared in a 98% yield by only two-step simple transformation (Scheme 26). In 2014, Aksenov and coworkers reported an interesting synthesis of 3-substituted 2-quinolines based on the addition of the nucleophile, ring opening, and ring closure (ANRORC)-type ring expansion [113] of the hydroxamic acid 92. The intermediate 92 was derived from the Fischer reaction of hydrazines 90 and 4-nitroketones 91 [114]. The optimized cyclization conditions used polyphosphoric acid (80% PPA) at 100-110 °C. The desired products were obtained with both electron-rich and -poor substituents at the C3 position of 88 including aryl-and alkyl groups and even with C3-nonsubstituted 4-nitroketones. On the basis of the previous report [113], a possible mechanism involving 5 to 6 ANRORC-type ring expansions of in situ generated hydroxamic acid  In 2016, Yu and coworkers published a practical method of base-mediated lactamization of sp 2 C-H bond with CO2 to access 2-quinolones 104 in good to excellent yields [117]. Note that carbon dioxide was used as a carbonyl source of quinolone scaffolds. The reaction with diglyme as a solvent was most effective and the use of alkali metal tert-butoxides as a base gave better yields than those with other bases (NaH, KOH, and DBU). Several alkali metal tert-butoxides were screened and NaOtBu was found to be superior to other bases, such as LiOtBu and KOtBu, providing a 95% yield at 140 °C. Various substituted anilines with ortho-alkenyl moiety were successfully converted to 4substituted 2-quinolones in moderate to excellent yields. In particular, the ortho α-styrenyl anilines reacted with CO2 to afford 4-aryl-2-quinolones. The synthetic utility of the method was demonstrated by the synthesis of 6-chloro-4-phenyl-2-quinolone (HBV inhibitor). The proposed mechanistic pathway is depicted in Scheme 30. During the mechanistic studies, through some control experiments, the compounds such as II-IV were observed. Although not conclusive, the key intermediate to allow for cyclization seems to be the isocyanate III. In 2016, Yu and coworkers published a practical method of base-mediated lactamization of sp 2 C-H bond with CO 2 to access 2-quinolones 104 in good to excellent yields [117]. Note that carbon dioxide was used as a carbonyl source of quinolone scaffolds. The reaction with diglyme as a solvent was most effective and the use of alkali metal tert-butoxides as a base gave better yields than those with other bases (NaH, KOH, and DBU). Several alkali metal tert-butoxides were screened and NaOtBu was found to be superior to other bases, such as LiOtBu and KOtBu, providing a 95% yield at 140 • C. Various substituted anilines with ortho-alkenyl moiety were successfully converted to 4-substituted 2-quinolones in moderate to excellent yields. In particular, the ortho α-styrenyl anilines reacted with CO 2 to afford 4-aryl-2-quinolones. The synthetic utility of the method was demonstrated by the synthesis of 6-chloro-4-phenyl-2-quinolone (HBV inhibitor). The proposed mechanistic pathway is depicted in Scheme 30. During the mechanistic studies, through some control experiments, the compounds such as II-IV were observed. Although not conclusive, the key intermediate to allow for cyclization seems to be the isocyanate III. In 2016, Yu and coworkers published a practical method of base-mediated lactamization of sp 2 C-H bond with CO2 to access 2-quinolones 104 in good to excellent yields [117]. Note that carbon dioxide was used as a carbonyl source of quinolone scaffolds. The reaction with diglyme as a solvent was most effective and the use of alkali metal tert-butoxides as a base gave better yields than those with other bases (NaH, KOH, and DBU). Several alkali metal tert-butoxides were screened and NaOtBu was found to be superior to other bases, such as LiOtBu and KOtBu, providing a 95% yield at 140 °C. Various substituted anilines with ortho-alkenyl moiety were successfully converted to 4substituted 2-quinolones in moderate to excellent yields. In particular, the ortho α-styrenyl anilines reacted with CO2 to afford 4-aryl-2-quinolones. The synthetic utility of the method was demonstrated by the synthesis of 6-chloro-4-phenyl-2-quinolone (HBV inhibitor). The proposed mechanistic pathway is depicted in Scheme 30. During the mechanistic studies, through some control experiments, the compounds such as II-IV were observed. Although not conclusive, the key intermediate to allow for cyclization seems to be the isocyanate III.

Pd-Catalyzed Reactions
Palladium-catalyzed carbonylative annulation of alkynes is a representative synthetic pathway to prepare 2-quinolones. In 2003, the Larock research group reported the first palladium-catalyzed synthesis of 2-quinolones using three components, an o-iodoaniline 105, a terminal alkyne 106, and carbon monoxide gas [118]. Mixtures of 3-and 4-substituted 2-quinolones, 107 and 108, were obtained when the terminal alkyne bore more than four carbon alkyl chains. Moreover, 3-substituted 2-quinolones were predominant over 4-substituted ones and the ratios of the two regioisomers were greater than 69:31 in all cases (Scheme 31). Interestingly, the terminal alkynes with alkyl alcohol, ester, or nitrile provided the desired products in better regioselectivity up to 81:19. These results can be explained by the fact that the palladium complex is further stabilized by additional coordination of the palladium atom with the functional group of the alkyne. The reaction with phenyl acetylene provided the product in excellent regioselectivity, even though polymeric byproducts were formed significantly.

Pd-Catalyzed Reactions
Palladium-catalyzed carbonylative annulation of alkynes is a representative synthetic pathway to prepare 2-quinolones. In 2003, the Larock research group reported the first palladium-catalyzed synthesis of 2-quinolones using three components, an o-iodoaniline 105, a terminal alkyne 106, and carbon monoxide gas [118]. Mixtures of 3-and 4-substituted 2-quinolones, 107 and 108, were obtained when the terminal alkyne bore more than four carbon alkyl chains. Moreover, 3-substituted 2quinolones were predominant over 4-substituted ones and the ratios of the two regioisomers were greater than 69:31 in all cases (Scheme 31). Interestingly, the terminal alkynes with alkyl alcohol, ester, or nitrile provided the desired products in better regioselectivity up to 81:19. These results can be explained by the fact that the palladium complex is further stabilized by additional coordination of the palladium atom with the functional group of the alkyne. The reaction with phenyl acetylene provided the product in excellent regioselectivity, even though polymeric byproducts were formed significantly. Scheme 31. Palladium-catalyzed annulation of terminal alkynes. Furthermore, 3,4-disubstituted 2-quinolones 111 could be prepared using internal alkynes 110 in the presence of carbon monoxide gas through palladium-catalyzed carbonylative annulation [119]. N-Protected o-iodoanilines 109 are utilized in this process, and the choice of protecting groups was important. After completion of the reaction, the protecting groups were removed by treatment of 1 M ethanolic NaOH to provide the unprotected 2-quinolones. Symmetric internal alkynes with alkyl, (hetero)aryl, alkoxyl, and hydroxyl groups gave the desired products in moderate to good yields. However, mixtures of regioisomers with poor selectivity due to two possible modes of alkyne insertion were obtained when unsymmetrical alkynes were employed (Scheme 32). It is suggested that the regioselectivity is determined based on the steric factors of two different substituents of alkynes. Less steric repulsion is expected in the palladium complex I than II between the larger substituent of the alkyne and palladium because of the longer palladium-carbon bond compared to the carbon-carbon bond in these two complexes. Carbonylative reactions using carbon monoxide gas have some drawbacks due to high toxicity and difficulties in the handling of gaseous carbon Scheme 31. Palladium-catalyzed annulation of terminal alkynes. Furthermore, 3,4-disubstituted 2-quinolones 111 could be prepared using internal alkynes 110 in the presence of carbon monoxide gas through palladium-catalyzed carbonylative annulation [119]. N-Protected o-iodoanilines 109 are utilized in this process, and the choice of protecting groups was important. After completion of the reaction, the protecting groups were removed by treatment of 1 M ethanolic NaOH to provide the unprotected 2-quinolones. Symmetric internal alkynes with alkyl, (hetero)aryl, alkoxyl, and hydroxyl groups gave the desired products in moderate to good yields. However, mixtures of regioisomers with poor selectivity due to two possible modes of alkyne insertion were obtained when unsymmetrical alkynes were employed (Scheme 32). It is suggested that the regioselectivity is determined based on the steric factors of two different substituents of alkynes. Less steric repulsion is expected in the palladium complex I than II between the larger substituent of the alkyne and palladium because of the longer palladium-carbon bond compared to the carbon-carbon bond in these two complexes. Carbonylative reactions using carbon monoxide gas have some drawbacks due to high toxicity and difficulties in the handling of gaseous carbon monoxide.
As an alternative to carbon monoxide gas, a solid carbon monoxide source, Mo(CO) 6 , has been used in carbonylative reactions, and carbon monoxide is generated in situ during the course of the reactions. monoxide. As an alternative to carbon monoxide gas, a solid carbon monoxide source, Mo(CO)6, has been used in carbonylative reactions, and carbon monoxide is generated in situ during the course of the reactions.  monoxide. As an alternative to carbon monoxide gas, a solid carbon monoxide source, Mo(CO)6, has been used in carbonylative reactions, and carbon monoxide is generated in situ during the course of the reactions.  The Otaredi-Kashani group also reported CO gas-free synthesis of 2-quinolones [121]. The palladium-catalyzed carbonylative annulation with N-protected o-iodoanilines 115 and internal The Otaredi-Kashani group also reported CO gas-free synthesis of 2-quinolones [121]. The palladiumcatalyzed carbonylative annulation with N-protected o-iodoanilines 115 and internal alkynes 116 in the presence of molybdenum hexacarbonyl, Mo(CO) 6 , provided 3,4-disubstituted 2-quinolones 117 in good yields (Scheme 34). Due to the low yields with unprotected anilines, various N-protecting groups such as -CO 2 Et, -Ts, -Ms, and -COCF 3 of the aniline were explored, and deprotection proceeded in the course of the reaction to provide the unprotected 2-quinolones 117. Unfortunately, only a trace amount of the desired product was obtained when using diphenylacetylene because of homocoupling and polymerization.
Molecules 2020, 25, x FOR PEER REVIEW 26 of 42 alkynes 116 in the presence of molybdenum hexacarbonyl, Mo(CO)6, provided 3,4-disubstituted 2quinolones 117 in good yields (Scheme 34). Due to the low yields with unprotected anilines, various N-protecting groups such as -CO2Et, -Ts, -Ms, and -COCF3 of the aniline were explored, and deprotection proceeded in the course of the reaction to provide the unprotected 2-quinolones 117.
Unfortunately, only a trace amount of the desired product was obtained when using diphenylacetylene because of homocoupling and polymerization.
As an alternative to carbon monoxide gas, oxalic acid dihydrate 124, (COOH)2•2H2O, was used as an in situ generated carbon monoxide source for the synthesis of 2-quinolones 125 (Scheme 36). Das and coworkers used heterogeneous polystyrene-supported palladium, Pd@PS, as a catalyst to effectively hold the (COOH)2•2H2O under microwave-assisted reaction conditions [123]. Interestingly, the Pd@PS catalyst can be recycled up to four times without loss of activity. Moreover, 3-substituted 2quinolones 125 were obtained in a regioselective manner and 4-substituted regioisomers were not detected. . Due to the low yields with unprotected anilines, various N-protecting groups such as -CO2Et, -Ts, -Ms, and -COCF3 of the aniline were explored, and deprotection proceeded in the course of the reaction to provide the unprotected 2-quinolones 117.
Unfortunately, only a trace amount of the desired product was obtained when using diphenylacetylene because of homocoupling and polymerization.
As an alternative to carbon monoxide gas, oxalic acid dihydrate 124, (COOH)2•2H2O, was used as an in situ generated carbon monoxide source for the synthesis of 2-quinolones 125 (Scheme 36). Das and coworkers used heterogeneous polystyrene-supported palladium, Pd@PS, as a catalyst to effectively hold the (COOH)2•2H2O under microwave-assisted reaction conditions [123]. Interestingly, the Pd@PS catalyst can be recycled up to four times without loss of activity. Moreover, 3-substituted 2quinolones 125 were obtained in a regioselective manner and 4-substituted regioisomers were not detected.
As an alternative to carbon monoxide gas, oxalic acid dihydrate 124, (COOH) 2 ·2H 2 O, was used as an in situ generated carbon monoxide source for the synthesis of 2-quinolones 125 (Scheme 36). Das and coworkers used heterogeneous polystyrene-supported palladium, Pd@PS, as a catalyst to effectively hold the (COOH) 2 ·2H 2 O under microwave-assisted reaction conditions [123]. Interestingly, the Pd@PS catalyst can be recycled up to four times without loss of activity. Moreover, 3-substituted 2-quinolones 125 were obtained in a regioselective manner and 4-substituted regioisomers were not detected. A cascade reaction of palladium-catalyzed intermolecular aminocarbonylation followed by intramolecular amidation was reported by Willis' group in 2009 [124]. Here, 2-(2-bromoalkenyl)aryl bromide 126 effectively reacted with a variety of primary amines 127 in the presence of atmospheric pressure of carbon monoxide under the palladium-catalyzed reaction conditions. Depending on the substituents on the amines, various N-alkyl-, aryl-, or carbonylated 2-quinolones 128 were obtained in moderate to good yields (Scheme 37). Between two possible bromides, an alkenyl-and aryl bromide, of 2-(2-bromoalkenyl)aryl bromide 126, the alkenyl bromide reacts first with the palladium catalyst followed by carbonylation and then amination to give the corresponding 2-quinolones. Carbon dioxide, a greener source of a carbonyl moiety compared to hazardous CO gas, can be also used as a carbonyl fragment in the synthesis of 2-quinolones. Cheng and coworkers reported the synthesis of 4-aryl 2-quinolones 131 using o-iodoanilines 129, N-tosylhydrazones 130, and carbon dioxide in the presence of a palladium catalyst [125]. In the presence of a base, N-tosylhydrazones converts to diazo substrates to generate palladium carbene intermediate, whose catalytic cycle was followed by migratory insertion, reaction with CO2, and 6π-electrocyclic reaction to provide the Scheme 36. Regioselective synthesis of 3-substituted 2-quinolone using Pd@PS.
A cascade reaction of palladium-catalyzed intermolecular aminocarbonylation followed by intramolecular amidation was reported by Willis' group in 2009 [124]. Here, 2-(2-bromoalkenyl)aryl bromide 126 effectively reacted with a variety of primary amines 127 in the presence of atmospheric pressure of carbon monoxide under the palladium-catalyzed reaction conditions. Depending on the substituents on the amines, various N-alkyl-, aryl-, or carbonylated 2-quinolones 128 were obtained in moderate to good yields (Scheme 37). Between two possible bromides, an alkenyl-and aryl bromide, of 2-(2-bromoalkenyl)aryl bromide 126, the alkenyl bromide reacts first with the palladium catalyst followed by carbonylation and then amination to give the corresponding 2-quinolones. A cascade reaction of palladium-catalyzed intermolecular aminocarbonylation followed by intramolecular amidation was reported by Willis' group in 2009 [124]. Here, 2-(2-bromoalkenyl)aryl bromide 126 effectively reacted with a variety of primary amines 127 in the presence of atmospheric pressure of carbon monoxide under the palladium-catalyzed reaction conditions. Depending on the substituents on the amines, various N-alkyl-, aryl-, or carbonylated 2-quinolones 128 were obtained in moderate to good yields (Scheme 37). Between two possible bromides, an alkenyl-and aryl bromide, of 2-(2-bromoalkenyl)aryl bromide 126, the alkenyl bromide reacts first with the palladium catalyst followed by carbonylation and then amination to give the corresponding 2-quinolones. Carbon dioxide, a greener source of a carbonyl moiety compared to hazardous CO gas, can be also used as a carbonyl fragment in the synthesis of 2-quinolones. Cheng and coworkers reported the synthesis of 4-aryl 2-quinolones 131 using o-iodoanilines 129, N-tosylhydrazones 130, and carbon dioxide in the presence of a palladium catalyst [125]. In the presence of a base, N-tosylhydrazones converts to diazo substrates to generate palladium carbene intermediate, whose catalytic cycle was followed by migratory insertion, reaction with CO2, and 6π-electrocyclic reaction to provide the Carbon dioxide, a greener source of a carbonyl moiety compared to hazardous CO gas, can be also used as a carbonyl fragment in the synthesis of 2-quinolones. Cheng and coworkers reported the synthesis of 4-aryl 2-quinolones 131 using o-iodoanilines 129, N-tosylhydrazones 130, and carbon dioxide in the presence of a palladium catalyst [125]. In the presence of a base, N-tosylhydrazones converts to diazo substrates to generate palladium carbene intermediate, whose catalytic cycle was followed by migratory insertion, reaction with CO 2 , and 6π-electrocyclic reaction to provide the desired 2-quinolone products. A variety of 4-aryl substituted or 3,4-disubstituted 2-quinolones were obtained based on the substitution patterns of N-tosylhydrazones in moderate to good yields (Scheme 38).

Other Transition-Metal-Catalyzed Three-Component Reactions
In 2015, Jiao and coworkers reported Rh-catalyzed 2-quinolone synthesis with anilines 132, internal alkynes 133, and carbon monoxide through C-H activation [126]. Cu(OAc)2 was used as an efficient oxidant in this process and Li2CO3 was used as a base. With the use of symmetric internal alkynes, 3,4-disubstituted 2-quinolones 134 were obtained in moderated to high yields. A broad range of functional groups were tolerated. By control experiment, rhodacycle II was prepared from the compound I in the presence of a stoichiometric amount of Rh(PPh3)3 and the structure II was determined by X-ray diffraction. Reaction between the rhodacycle II and dibutylacetylene provided the corresponding 2-quinolone III (Scheme 39).
Scheme 39. C-H activation of anilines with alkynes and CO gas.

Other Transition-Metal-Catalyzed Three-Component Reactions
In 2015, Jiao and coworkers reported Rh-catalyzed 2-quinolone synthesis with anilines 132, internal alkynes 133, and carbon monoxide through C-H activation [126]. Cu(OAc) 2 was used as an efficient oxidant in this process and Li 2 CO 3 was used as a base. With the use of symmetric internal alkynes, 3,4-disubstituted 2-quinolones 134 were obtained in moderated to high yields. A broad range of functional groups were tolerated. By control experiment, rhodacycle II was prepared from the compound I in the presence of a stoichiometric amount of Rh(PPh 3 ) 3 and the structure II was determined by X-ray diffraction. Reaction between the rhodacycle II and dibutylacetylene provided the corresponding 2-quinolone III (Scheme 39).

Other Transition-Metal-Catalyzed Three-Component Reactions
In 2015, Jiao and coworkers reported Rh-catalyzed 2-quinolone synthesis with anilines 132, internal alkynes 133, and carbon monoxide through C-H activation [126]. Cu(OAc)2 was used as an efficient oxidant in this process and Li2CO3 was used as a base. With the use of symmetric internal alkynes, 3,4-disubstituted 2-quinolones 134 were obtained in moderated to high yields. A broad range of functional groups were tolerated. By control experiment, rhodacycle II was prepared from the compound I in the presence of a stoichiometric amount of Rh(PPh3)3 and the structure II was determined by X-ray diffraction. Reaction between the rhodacycle II and dibutylacetylene provided the corresponding 2-quinolone III (Scheme 39). Jiao and coworkers also reported Rh-catalyzed aerobic oxidative synthesis of 2-quinolones with anilines 135, internal alkynes 136, and carbon monoxide [127]. Even though synthetic challenges exist due to the sensitivity of amines to strong oxidants, primary-and tertiary amines were successfully utilized in this Rh-catalyzed aerobic oxidative cyclization. In the proposed catalytic cycle, Rh(I) can be oxidized to Rh(III) by Cu(II); then, the resulting Cu(I) catalyst is re-oxidized to Cu(II) in the presence of O 2 and carbon monoxide. By the DFT calculation, a rhodium catalyst is required for the efficient transformation, and palladium or iridium were found to be inefficient in this process because of a high energetic barrier. During optimization of the catalytic system, the best ratio of CO/O 2 was 2:1 (1 atm) for tertiary amines and 4:1 (1 atm) for primary/secondary amines. It is notable that a catalytic amount of dppb was required in cases using tertiary anilines. When asymmetric aryl alkyl alkynes were used, mixtures of regioisomers were obtained in good yields with moderate regioselectivity (Scheme 40a). When primary and secondary anilines were used, the corresponding 2-quinolones were generated in moderated to good yields as well (Scheme 40b).
Molecules 2020, 25, x FOR PEER REVIEW 29 of 42 Jiao and coworkers also reported Rh-catalyzed aerobic oxidative synthesis of 2-quinolones with anilines 135, internal alkynes 136, and carbon monoxide [127]. Even though synthetic challenges exist due to the sensitivity of amines to strong oxidants, primary-and tertiary amines were successfully utilized in this Rh-catalyzed aerobic oxidative cyclization. In the proposed catalytic cycle, Rh(I) can be oxidized to Rh(III) by Cu(II); then, the resulting Cu(I) catalyst is re-oxidized to Cu(II) in the presence of O2 and carbon monoxide. By the DFT calculation, a rhodium catalyst is required for the efficient transformation, and palladium or iridium were found to be inefficient in this process because of a high energetic barrier. During optimization of the catalytic system, the best ratio of CO/O2 was 2:1 (1 atm) for tertiary amines and 4:1 (1 atm) for primary/secondary amines. It is notable that a catalytic amount of dppb was required in cases using tertiary anilines. When asymmetric aryl alkyl alkynes were used, mixtures of regioisomers were obtained in good yields with moderate regioselectivity (Scheme 40a). When primary and secondary anilines were used, the corresponding 2-quinolones were generated in moderated to good yields as well (Scheme 40b). Ir-Catalyzed C-H/N-H activation with anilines 140, internal alkynes 141, and carbon monoxide for the synthesis of 2-quinolones was reported by Wu's group in 2016 [128]. Even though the reaction required high pressure of carbon monoxide (30 bar), a wide range of alkyl-, aryl-, or heteroaryl 3,4disubstituted 2-quinolone derivatives were obtained by Ir-catalyzed carbonylative annulation (Scheme 41). The developed reaction featured no requirements for preactivation or directing groups. The proposed mechanism included a five-membered iridacycle as the key intermediate, which is similar to the rhodacycle reported by Jiao et al. [127]. Ir-Catalyzed C-H/N-H activation with anilines 140, internal alkynes 141, and carbon monoxide for the synthesis of 2-quinolones was reported by Wu's group in 2016 [128]. Even though the reaction required high pressure of carbon monoxide (30 bar), a wide range of alkyl-, aryl-, or heteroaryl 3,4-disubstituted 2-quinolone derivatives were obtained by Ir-catalyzed carbonylative annulation (Scheme 41). The developed reaction featured no requirements for preactivation or directing groups. The proposed mechanism included a five-membered iridacycle as the key intermediate, which is similar to the rhodacycle reported by Jiao et al. [127]. Recently, Kim and Lim reported Cu-catalyzed synthesis of 2-quinolones by SN2, Knoevenagel condensation, and C-N bond forming process [129]. Functionalization with various sulfonyl groups on the 3-position of 2-quinolones was enabled by employing various aryl-or alkylsulfinate sodium salts (Scheme 42). In addition to sulfur(II), use of other nucleophiles such as a phenoxide, thiolate, and an aniline was also demonstrated. Compared to other three-component reactions, the carbonyl moiety of 2-quinolones stems from amides 141, not from carbon monoxide or its equivalents. Even though longer reaction time (2 days) was required, 3,4-disubstituted 2-quinolones could be synthesized in a straightforward manner.

Four-Component Reactions and Related Protocols
Whereas numerous examples using two-or three-component reactions have been reported for 2-quinolone synthesis, more complicated systems using four, five, or six components are rare. The established protocol for the multi-component reaction represents a Ugi four-component reaction that uses an amine, isocyanide, aldehyde, and carboxylic acid. This cascade reaction can afford 1,4bisamide products. Several interesting procedures employing the Ugi reaction were identified, as Recently, Kim and Lim reported Cu-catalyzed synthesis of 2-quinolones by S N 2, Knoevenagel condensation, and C-N bond forming process [129]. Functionalization with various sulfonyl groups on the 3-position of 2-quinolones was enabled by employing various aryl-or alkylsulfinate sodium salts (Scheme 42). In addition to sulfur(II), use of other nucleophiles such as a phenoxide, thiolate, and an aniline was also demonstrated. Compared to other three-component reactions, the carbonyl moiety of 2-quinolones stems from amides 141, not from carbon monoxide or its equivalents. Even though longer reaction time (2 days) was required, 3,4-disubstituted 2-quinolones could be synthesized in a straightforward manner. Recently, Kim and Lim reported Cu-catalyzed synthesis of 2-quinolones by SN2, Knoevenagel condensation, and C-N bond forming process [129]. Functionalization with various sulfonyl groups on the 3-position of 2-quinolones was enabled by employing various aryl-or alkylsulfinate sodium salts (Scheme 42). In addition to sulfur(II), use of other nucleophiles such as a phenoxide, thiolate, and an aniline was also demonstrated. Compared to other three-component reactions, the carbonyl moiety of 2-quinolones stems from amides 141, not from carbon monoxide or its equivalents. Even though longer reaction time (2 days) was required, 3,4-disubstituted 2-quinolones could be synthesized in a straightforward manner.

Four-Component Reactions and Related Protocols
Whereas numerous examples using two-or three-component reactions have been reported for 2-quinolone synthesis, more complicated systems using four, five, or six components are rare. The established protocol for the multi-component reaction represents a Ugi four-component reaction that uses an amine, isocyanide, aldehyde, and carboxylic acid. This cascade reaction can afford 1,4bisamide products. Several interesting procedures employing the Ugi reaction were identified, as

Four-Component Reactions and Related Protocols
Whereas numerous examples using two-or three-component reactions have been reported for 2-quinolone synthesis, more complicated systems using four, five, or six components are rare. The established protocol for the multi-component reaction represents a Ugi four-component reaction that uses an amine, isocyanide, aldehyde, and carboxylic acid. This cascade reaction can afford 1,4-bisamide products. Several interesting procedures employing the Ugi reaction were identified, as described below, with more than three components to construct 2-quinolones. Some examples covered in this section are not directly related to the one-pot reaction; however, they are potentially important as one-pot protocols which can give distinct N-substitution of 2-quinolones.
In 2004, Torroba and coworkers reported a novel synthetic method for 3,4-disubstituted 2-quinolones that features a four-component reaction [130]. This one-pot cascade reaction was enabled by tandem Ugi/Knoevenagel reactions using 2-aminophenylketones 147, aldehydes 148, cyclohexylisocyanide 149, and malonic or sulfonylacetic acid derivatives 150 (Scheme 43). The first step involves the Ugi four-component reaction to form the bisamides 151 [131,132]. Then, 151 was cyclized by Knoevenagel condensation to afford the densely functionalized 2-quinolone derivatives 152. It was notable that spontaneously annulated products were observed when R 3 was nitrile or ester, whereas the bisamides themselves were isolated when R 3 was arylsulfonyl groups.
Molecules 2020, 25, x FOR PEER REVIEW 31 of 42 described below, with more than three components to construct 2-quinolones. Some examples covered in this section are not directly related to the one-pot reaction; however, they are potentially important as one-pot protocols which can give distinct N-substitution of 2-quinolones. In 2004, Torroba and coworkers reported a novel synthetic method for 3,4-disubstituted 2quinolones that features a four-component reaction [130]. This one-pot cascade reaction was enabled by tandem Ugi/Knoevenagel reactions using 2-aminophenylketones 147, aldehydes 148, cyclohexylisocyanide 149, and malonic or sulfonylacetic acid derivatives 150 (Scheme 43). The first step involves the Ugi four-component reaction to form the bisamides 151 [131,132]. Then, 151 was cyclized by Knoevenagel condensation to afford the densely functionalized 2-quinolone derivatives 152. It was notable that spontaneously annulated products were observed when R 3 was nitrile or ester, whereas the bisamides themselves were isolated when R 3 was arylsulfonyl groups. The related studies were found in an article in 2011 [133]. McCluskey and coworkers reported the formation of bisamides 154 by unexpected three-component Ugi reaction during the library synthesis of 2-quinolone derivatives. It was notable that the nucleophilicity of the carboxylic acid affected the selectivity towards three-vs. four-component reactions. As demonstrated in their studies, cyanoacetic acid gave a mixture of both Ugi three-and four-component reaction products. Interestingly, the four-component Ugi products were spontaneously converted to the corresponding quinolones 155 while the three-component Ugi products were isolated as monoamides 154. A reaction mechanism was proposed; a route to three-component reaction product 154 was suggested to be the addition of H2O to II, but hydrolysis of IV cannot be excluded (Scheme 44).

Scheme 44.
McCluskey's studies on four-component reaction. The related studies were found in an article in 2011 [133]. McCluskey and coworkers reported the formation of bisamides 154 by unexpected three-component Ugi reaction during the library synthesis of 2-quinolone derivatives. It was notable that the nucleophilicity of the carboxylic acid affected the selectivity towards three-vs. four-component reactions. As demonstrated in their studies, cyanoacetic acid gave a mixture of both Ugi three-and four-component reaction products. Interestingly, the four-component Ugi products were spontaneously converted to the corresponding quinolones 155 while the three-component Ugi products were isolated as monoamides 154. A reaction mechanism was proposed; a route to three-component reaction product 154 was suggested to be the addition of H 2 O to II, but hydrolysis of IV cannot be excluded (Scheme 44).
The next variant of this chemistry was reported by Foroumadi and coworkers [134]. Intramolecular Heck reaction was utilized with the precursors 156 prepared by Ugi four-component reaction. By employing 4-butenoic acid in the Ugi reaction, the requisite alkene moiety for the Heck reaction was installed and it reacted with aryl halide, affording N-alkyl 4-methyl-2-quinolone derivatives 157 after double bond isomerization (Scheme 45). The optimized conditions of the Heck reaction involved Pd(OAc) 2 , PPh 3 , and Cs 2 CO 3 in refluxing toluene.
affected the selectivity towards three-vs. four-component reactions. As demonstrated in their studies, cyanoacetic acid gave a mixture of both Ugi three-and four-component reaction products. Interestingly, the four-component Ugi products were spontaneously converted to the corresponding quinolones 155 while the three-component Ugi products were isolated as monoamides 154. A reaction mechanism was proposed; a route to three-component reaction product 154 was suggested to be the addition of H2O to II, but hydrolysis of IV cannot be excluded (Scheme 44). The next variant of this chemistry was reported by Foroumadi and coworkers [134]. Intramolecular Heck reaction was utilized with the precursors 156 prepared by Ugi four-component reaction. By employing 4-butenoic acid in the Ugi reaction, the requisite alkene moiety for the Heck reaction was installed and it reacted with aryl halide, affording N-alkyl 4-methyl-2-quinolone derivatives 157 after double bond isomerization (Scheme 45). The optimized conditions of the Heck reaction involved Pd(OAc)2, PPh3, and Cs2CO3 in refluxing toluene.

Scheme 45. Preparation of 2-quinolones using four-component adduct via Heck reaction.
A similar four-component reaction was utilized by Peshkov and coworkers. Alkynic acids were used to form key intermediates 162 for preparation of 4-substituted 2-quinolones 163 [135]. While several types of hydroarylation reaction by alkyne activation to construct the lactam nucleus of 2quinolone were reported, the authors sought diversity of N-substituents in this transformation; their choice for N-variation was the Ugi reaction. As illustrated in Scheme 46, the diversely N-substituted precursors 162 were prepared for the Au-catalyzed annulation. Recent examples of utilizing the Ugi adducts were reported by Sharma and coworkers in 2019 [136]. In their work, a similar 6-endo-dig hydroarylation was designed by employing cationic Pd-catalysts to furnish 2-quinolones. Scheme 46. π-Acid-catalyzed cyclization of four-component adducts containing alkynes.
The most recent example of the Ugi reaction was presented by Dömling and coworkers [137]. Indole-2-carboxylic acids 164 can be used as a platform for the preparation of the tetracyclic scaffolds 166. The key intermediates 168 were subjected to Pd-catalyzed dual C-H bond functionalization reactions and, accordingly, fused tetracyclic indoloquinolones 169 were obtained in moderate yields (Scheme 47). The use of the readily available building blocks as starting materials is an important feature of this method, which likely allows for a modular approach. Moreover, the prepared material 169 was evaluated in an in silico docking model to find potential kinase inhibitors. A similar four-component reaction was utilized by Peshkov and coworkers. Alkynic acids were used to form key intermediates 162 for preparation of 4-substituted 2-quinolones 163 [135]. While several types of hydroarylation reaction by alkyne activation to construct the lactam nucleus of 2-quinolone were reported, the authors sought diversity of N-substituents in this transformation; their choice for N-variation was the Ugi reaction. As illustrated in Scheme 46, the diversely N-substituted precursors 162 were prepared for the Au-catalyzed annulation. Recent examples of utilizing the Ugi adducts were reported by Sharma and coworkers in 2019 [136]. In their work, a similar 6-endo-dig hydroarylation was designed by employing cationic Pd-catalysts to furnish 2-quinolones. The next variant of this chemistry was reported by Foroumadi and coworkers [134]. Intramolecular Heck reaction was utilized with the precursors 156 prepared by Ugi four-component reaction. By employing 4-butenoic acid in the Ugi reaction, the requisite alkene moiety for the Heck reaction was installed and it reacted with aryl halide, affording N-alkyl 4-methyl-2-quinolone derivatives 157 after double bond isomerization (Scheme 45). The optimized conditions of the Heck reaction involved Pd(OAc)2, PPh3, and Cs2CO3 in refluxing toluene.

Scheme 45. Preparation of 2-quinolones using four-component adduct via Heck reaction.
A similar four-component reaction was utilized by Peshkov and coworkers. Alkynic acids were used to form key intermediates 162 for preparation of 4-substituted 2-quinolones 163 [135]. While several types of hydroarylation reaction by alkyne activation to construct the lactam nucleus of 2quinolone were reported, the authors sought diversity of N-substituents in this transformation; their choice for N-variation was the Ugi reaction. As illustrated in Scheme 46, the diversely N-substituted precursors 162 were prepared for the Au-catalyzed annulation. Recent examples of utilizing the Ugi adducts were reported by Sharma and coworkers in 2019 [136]. In their work, a similar 6-endo-dig hydroarylation was designed by employing cationic Pd-catalysts to furnish 2-quinolones. Scheme 46. π-Acid-catalyzed cyclization of four-component adducts containing alkynes.
The most recent example of the Ugi reaction was presented by Dömling and coworkers [137]. Indole-2-carboxylic acids 164 can be used as a platform for the preparation of the tetracyclic scaffolds 166. The key intermediates 168 were subjected to Pd-catalyzed dual C-H bond functionalization reactions and, accordingly, fused tetracyclic indoloquinolones 169 were obtained in moderate yields (Scheme 47). The use of the readily available building blocks as starting materials is an important feature of this method, which likely allows for a modular approach. Moreover, the prepared material 169 was evaluated in an in silico docking model to find potential kinase inhibitors. Scheme 46. π-Acid-catalyzed cyclization of four-component adducts containing alkynes.
The most recent example of the Ugi reaction was presented by Dömling and coworkers [137]. Indole-2-carboxylic acids 164 can be used as a platform for the preparation of the tetracyclic scaffolds 166. The key intermediates 168 were subjected to Pd-catalyzed dual C-H bond functionalization reactions and, accordingly, fused tetracyclic indoloquinolones 169 were obtained in moderate yields (Scheme 47). The use of the readily available building blocks as starting materials is an important feature of this method, which likely allows for a modular approach. Moreover, the prepared material 169 was evaluated in an in silico docking model to find potential kinase inhibitors.

Recent Approaches to Fused 2-Quinolones
As demonstrated in two-component reactions, arynes could participate in the cyclization via a cascade C-H/N-H bond activation. In 2018, Zhang and coworkers developed the protocol to furnish tetracyclic indoloquinolones 172 via Cu(II)-mediated annulation using N-quinolyl indole-3carboxamides 170 and in situ generated arynes from benzyne precursors 171 [138]. In this way, medicinally important indoloquinoline derivatives were readily accessible; as an application of the method, formal synthesis of the indoloquinoline alkaloid such as isocryptolepine (natural antimalarial) was accomplished. The proposed mechanism is depicted in Scheme 48. Through some control experiments including a primary intermolecular KIE (value = 1.3) and radical trapping with TEMPO, a SET mechanism was excluded. Instead, the key concerted metalation/deprotonation (CMD) pathway was proposed to form the corresponding metallacycle III, which in turn underwent carbocupration with the aryne (III→IV) and reductive elimination (IV→V).

Recent Approaches to Fused 2-Quinolones
As demonstrated in two-component reactions, arynes could participate in the cyclization via a cascade C-H/N-H bond activation. In 2018, Zhang and coworkers developed the protocol to furnish tetracyclic indoloquinolones 172 via Cu(II)-mediated annulation using N-quinolyl indole-3-carboxamides 170 and in situ generated arynes from benzyne precursors 171 [138]. In this way, medicinally important indoloquinoline derivatives were readily accessible; as an application of the method, formal synthesis of the indoloquinoline alkaloid such as isocryptolepine (natural antimalarial) was accomplished. The proposed mechanism is depicted in Scheme 48. Through some control experiments including a primary intermolecular KIE (value = 1.3) and radical trapping with TEMPO, a SET mechanism was excluded. Instead, the key concerted metalation/deprotonation (CMD) pathway was proposed to form the corresponding metallacycle III, which in turn underwent carbocupration with the aryne (III→IV) and reductive elimination (IV→V).
In 2018, Jiang and coworkers developed the regiodivergent carbonylation of dinucleophilic diarylalkynes 173 containing an alcohol and an amine; regioselective control was enabled by a Pd(II) catalyst and bidentate ligands [139]. Depending on the ligands, different regioisomers were synthesized; benzofuro[3,2-c]quinolones 174 were obtained by the use of an electron-deficient 1,10-phenanthroline-5,6-dione (L1) and indolo [3,2-c]coumarins 175 were obtained by use of a sterically bulky and electron-rich ligand, dppm. Due to the merit of divergent method, many structurally fascinating compounds were exemplified (Scheme 49a). It is notable that this method was also applicable to the substrates containing an amine and carboxylic acid as alternative nucleophiles instead of an alcohol. The reactions using them afforded indoloquinolones, pyranoquinolones, and 3,4-fused pentacyclic 2-quinolones in one pot (Scheme 49b). medicinally important indoloquinoline derivatives were readily accessible; as an application of the method, formal synthesis of the indoloquinoline alkaloid such as isocryptolepine (natural antimalarial) was accomplished. The proposed mechanism is depicted in Scheme 48. Through some control experiments including a primary intermolecular KIE (value = 1.3) and radical trapping with TEMPO, a SET mechanism was excluded. Instead, the key concerted metalation/deprotonation (CMD) pathway was proposed to form the corresponding metallacycle III, which in turn underwent carbocupration with the aryne (III→IV) and reductive elimination (IV→V). In 2018, Jiang and coworkers developed the regiodivergent carbonylation of dinucleophilic diarylalkynes 173 containing an alcohol and an amine; regioselective control was enabled by a Pd(II) catalyst and bidentate ligands [139]. Depending on the ligands, different regioisomers were synthesized; benzofuro[3,2-c]quinolones 174 were obtained by the use of an electron-deficient 1,10phenanthroline-5,6-dione (L1) and indolo [3,2-c]coumarins 175 were obtained by use of a sterically bulky and electron-rich ligand, dppm. Due to the merit of divergent method, many structurally fascinating compounds were exemplified (Scheme 49a). It is notable that this method was also applicable to the substrates containing an amine and carboxylic acid as alternative nucleophiles instead of an alcohol. The reactions using them afforded indoloquinolones, pyranoquinolones, and 3,4-fused pentacyclic 2-quinolones in one pot (Scheme 49b).

Conclusions and Outlook
In this review, we give an overview of recent synthetic efforts for the construction of 2-quinolone scaffolds. The contents were classified by the number of reaction components and the type of transition metals when applied. The early synthetic chemistry of 2-quinolones consists basically of variations on two types of reactions: Friedländer reaction and Knorr reaction. However, recent advances in new bond-forming reactions led to new approaches via unconventional designs. It Scheme 49. Ligand-controlled regioselective synthesis of 3,4-fused-2-quinolones.

Conclusions and Outlook
In this review, we give an overview of recent synthetic efforts for the construction of 2-quinolone scaffolds. The contents were classified by the number of reaction components and the type of transition metals when applied. The early synthetic chemistry of 2-quinolones consists basically of variations on two types of reactions: Friedländer reaction and Knorr reaction. However, recent advances in new bond-forming reactions led to new approaches via unconventional designs. It should be noted that non-classical access to 2-quinolones was greatly expanded after the introduction of the Pd-catalyzed two-component reaction by R.F. Heck in 1978. In this process, ortho-iodoanilines and 1,2-disubstituted olefins were elaborated into 2-quinolones through the palladium-catalyzed vinylic substitution reaction.
In many cases, for the synthesis of 2-quinolones, the palladium-catalyzed cross-coupling reactions between two components appeared to be crucial methods. The palladium-catalyzed cross-coupling chemistry has been further combined with the recently evolved palladium-catalyzed C-H activation and tandem coupling process for the synthesis of more complex and multi-substituted 2-quinolones. While palladium is still the most exploited transition metal for the synthesis of 2-quinolones, many efforts have been directed toward finding other transition metals. Significant advances have been made in the copper-catalyzed two-component reactions over the last few decades by replacing Pd-catalyzed reactions; Ullmann type C-N bond forming reactions were adopted for synthesis of the lactam nucleus in 2-quinolones. Besides these, other transition metals such as Rh, Ru, Ag, and Ir were also successfully used as catalysts of two-component reactions, although only a few examples were demonstrated.
In 2003, Larock and coworkers reported a seminal work for the synthesis of 2-quinolones via Pd-catalyzed three-component reaction using o-aniline, alkyne, and carbon monoxide (CO) gas. Since then, the preparation of 2-quinolones by three-component reaction using other carbonyl equivalents was investigated. Among various carbonyl precursors including Mo(CO) 6 , oxalic acid hydrate, and carbon dioxide, molybdenum hexacarbonyl [Mo(CO) 6 ] was the most useful source when reaction scale-up required a solid source of carbon monoxide. In these reaction settings, various transition metals (Pd, Cu, Rh, etc.) were employed with the complementary reaction scope to prepare multi-substituted 2-quinolone derivatives.
Although only one approach by the Ugi four-component reaction was found to provide 2-quinolones, it is believed that much more opportunities remain to develop novel methods using other types of fouror five-component reactions.
For decades, a series of pharmacologically important derivatives of 2-quinolones have been synthesized, mostly through facile two-component and three-component reactions. Not surprisingly, the development of synthetic methodologies of 2-quinolones is mostly gaining new momentum due to advances in transition metal chemistry. Nonetheless, the literature regarding 2-quinolone synthesis for medicinal purposes shows that only a few protocols are considered in the route-scouting from a practical point of view. Critical challenges still exist in the construction of a 2-quinolone core with readily available chemical feedstock and in the efficient introduction of substituents. We anticipate that the recent development of electrochemistry as well as photoredox chemistry will bring forth a range of improved transformations to functionalized 2-quinolones previously thought difficult to access.

Conflicts of Interest:
The authors declare no conflict of interest.