Caryocar brasiliense Cambess. Pulp Oil Supplementation Reduces Total Cholesterol, LDL-c, and Non-HDL-c in Animals

The fruit of Caryocar brasiliense Cambess. is a source of oil with active compounds that are protective to the organism. In our work, we analyzed the physicochemical characteristics and evaluated the effects of supplementation with C. brasiliense oil in an animal model. We characterized the oil by indices of quality and identity, optical techniques of absorption spectroscopy in the UV–Vis region and fluorescence, and thermogravimetry/derived thermogravimetry (TG/DTG). For the animal experiment, we utilized mice (Mus musculus) supplemented with lipidic source in different dosages. The results demonstrated that C. brasiliense oil is an alternative source for human consumption and presents excellent oxidative stability. Primarily, it exhibited oleic MFA (53.56%) and palmitic SFA (37.78%). The oil level of tocopherols and tocotrienols was superior to the carotenoids. The supplementation with C. brasiliense oil reduced the levels of total cholesterol, LDL-c, and non-HDL-c. Regarding visceral fats and adiposity index, the treatment synergically supplemented with olive oil and C. brasiliense oil (OO + CO) obtained the best result. Therefore, C. brasiliense oil is a high quality product for consumption. Its supplementation promotes beneficial effects mainly on the lipidic profile.

. Indices of quality and identity of Caryocar brasiliense pulp oil.

Index Values
Peroxide index (mEq O 2 kg −1 ) 13.63 ± 0.97 Acidity index in oleic acid (mg KOH/g −1 ) 1.27 ± 0.02 Saponification index (mg KOH g −1 ) 136.5 ± 0.60 Refraction index at 40 • C 1.46 ± 0.00 Iodine index (g I 2  The results are expressed as the means ± mean standard error. The peroxide index is an indicator of the initial stages of rancification and a measure of primary products of the lipidic oxidation [25]. The peroxide index presented a high value (13.63 mEq O 2 kg −1 ) that can be explained by the oil unsaturation degree, which may indicate the start of the oxidation process. However, the index is following the maximum standard for crude oils (<15 mEq O 2 kg −1 ), demonstrating adequacy for consumption [4]. Differing from the index of C. brasiliense, another study showed a result under 4.40 mEq O 2 kg −1 , found in C. coriaceum oil [28].
The iodine index is a measure of the unsaturation of fats and oils and consequentially the susceptibility to oxidation [29]. The obtained iodine index (76.7 I 2 /100 −1 g) is close to values found in analyses of 10 oils of different cultivars of olive (Olea europaea L.), between 80 and 89 I 2 /100 −1 g [30]. Its counterpart, the oil of the fruit of Byrsonima cydoniifolia A. Juss., also native to South America, has an iodine index with significantly higher unsaturation degree (120.84 I 2 /100 −1 g) [31], compared with C. brasiliense oil. This indicates higher stability of C. brasiliense compared with the others cited; the the higher the unsaturation, the lower its oxidative stability [25].
The saponification index is related to its molecular weight or chain length of triglycerides that compose the oil [32]. The reported result presented a low value (136.5 mg KOH g −1 ), near the recommended level for olive oil (184-196 mg KOH/g) and palm oil (190-209 mg KOH/g) [4,33], with characteristics similar to C. brasiliense. This indicates that the oil does not contain many fatty acids with low molecular weight.
Another parameter for oil characterization is it coloration, one of the initial factors pointing to possible lipidic oxidation and consequent degradation, as color alteration is caused by the degradation of essential FAs and others compounds [2]. The fruit of C. brasiliense is considered a source of carotenoids [23]; the liposoluble pigments responsible for the orange and red coloration [34].
The analyzed C. brasiliense oil evidenced the presence of carotenoids, due to the positive values of a* and b* ( Table 1), showing that most pigments are yellowish, followed by red, which is correlated with the total content of carotenoids detected in the samples. The high value of C* indicates a high-intensity color of the oil, thus considered dark. High contents of carotenoids are found in a limited number of edible oils, when compared with Mauritia flexuosa oil and palm oil, considered rich in this compound, with concentrations of 1722.87 mg kg −1 [35] and 1385 mg kg −1 , respectively [36]. C. brasiliense oil stands out, with a content of 2.39 µg/g of total carotenoids. In Figure 1 it is possible to observe the analysis of UV-Vis absorption and in Figure 2 the fluorescence of C. brasiliense oil at the concentration of 5 × 10 −3 g mL −1 (a) and the result of fluorescence in pure oil (b). We verified the presence of natural antioxidants, such as tocopherols, and tocotrienols, named vitamin E [37] by the absorption band approximately in 313 nm ( Figure 1) and the emission band in 327 nm, excited in 290 nm (Figure 2a).  With regard to the fluorescence analysis directly in undiluted vegetable oil (2b), we observed the presence of an emission band centered at 530 nm when excited at 470 nm. That fluorescence band can be attributed to the carotenoids [38]. The presence of chloropylls is usually emitted in the range of 650 to 750 nm [7,37], and was slightly ranked in the analysis of undiluted oil (Figure 2b). That datum is correlated with the value of total carotenoids found (Table 1), demonstrating the low concentration of carotenoids and higher presence of tocopherols C. brasiliense oil, with α-tocopherol standing out.
The thermal degradation ( Figure 3) of C. brasiliense oil was observed mainly in the range of 195 °C to 457 °C, later stabilizing its mass. The peak occurred at 388 °C, with a loss of total mass of 99.8%. The residues were 0.1%, below possible analytical errors. The loss of initial mass at ~195 °C can be attributed to moisture loss of the oil and the volatilization of compounds such as aldehydes and shortchain fatty acids, a common factor in vegetable oils, as the oil composition influences the total mass loss [39].  With regard to the fluorescence analysis directly in undiluted vegetable oil (2b), we observed the presence of an emission band centered at 530 nm when excited at 470 nm. That fluorescence band can be attributed to the carotenoids [38]. The presence of chloropylls is usually emitted in the range of 650 to 750 nm [7,37], and was slightly ranked in the analysis of undiluted oil (Figure 2b). That datum is correlated with the value of total carotenoids found (Table 1), demonstrating the low concentration of carotenoids and higher presence of tocopherols C. brasiliense oil, with α-tocopherol standing out.
The thermal degradation ( Figure 3) of C. brasiliense oil was observed mainly in the range of 195 °C to 457 °C, later stabilizing its mass. The peak occurred at 388 °C, with a loss of total mass of 99.8%. The residues were 0.1%, below possible analytical errors. The loss of initial mass at ~195 °C can be attributed to moisture loss of the oil and the volatilization of compounds such as aldehydes and shortchain fatty acids, a common factor in vegetable oils, as the oil composition influences the total mass loss [39]. With regard to the fluorescence analysis directly in undiluted vegetable oil (2b), we observed the presence of an emission band centered at 530 nm when excited at 470 nm. That fluorescence band can be attributed to the carotenoids [38]. The presence of chloropylls is usually emitted in the range of 650 to 750 nm [7,37], and was slightly ranked in the analysis of undiluted oil (Figure 2b). That datum is correlated with the value of total carotenoids found (Table 1), demonstrating the low concentration of carotenoids and higher presence of tocopherols C. brasiliense oil, with α-tocopherol standing out.
The thermal degradation ( Figure 3) of C. brasiliense oil was observed mainly in the range of 195 • C to 457 • C, later stabilizing its mass. The peak occurred at 388 • C, with a loss of total mass of 99.8%. The residues were 0.1%, below possible analytical errors. The loss of initial mass at~195 • C can be attributed to moisture loss of the oil and the volatilization of compounds such as aldehydes and short-chain fatty acids, a common factor in vegetable oils, as the oil composition influences the total mass loss [39].  The chemical composition of C. brasiliense oil includes antioxidant and oxidizable compounds that influence its oxidative stability [40]. The role of α-tocopherol is not yet totally defined, but it is known that its presence improves the oxidative stability in vegetable oils [3]. Moreover, it is considered one of the best phenolic antioxidants as it rapidly reacts with the alkyl peroxyl radical, forming more stable adducts and protecting lipids from peroxidation [41].
There is no minimum period of induction recommended for good quality edible oils; nevertheless, we consider that the C. brasiliense oil reached a long induction period of 8.6 h and consequent excellent oxidative stability (Figure 4), mainly because of the high presence of SFA and MFA and other minor components. This means C. brasiliense oil has a suitable shelf life. Similarly, the C. brasiliense pulp and nut oils evaluated by Torres et al. [21]    The chemical composition of C. brasiliense oil includes antioxidant and oxidizable compounds that influence its oxidative stability [40]. The role of α-tocopherol is not yet totally defined, but it is known that its presence improves the oxidative stability in vegetable oils [3]. Moreover, it is considered one of the best phenolic antioxidants as it rapidly reacts with the alkyl peroxyl radical, forming more stable adducts and protecting lipids from peroxidation [41].
There is no minimum period of induction recommended for good quality edible oils; nevertheless, we consider that the C. brasiliense oil reached a long induction period of 8.6 h and consequent excellent oxidative stability (Figure 4), mainly because of the high presence of SFA and MFA and other minor components. This means C. brasiliense oil has a suitable shelf life. Similarly, the C. brasiliense pulp and nut oils evaluated by Torres et al. [21]  The chemical composition of C. brasiliense oil includes antioxidant and oxidizable compounds that influence its oxidative stability [40]. The role of α-tocopherol is not yet totally defined, but it is known that its presence improves the oxidative stability in vegetable oils [3]. Moreover, it is considered one of the best phenolic antioxidants as it rapidly reacts with the alkyl peroxyl radical, forming more stable adducts and protecting lipids from peroxidation [41].
There is no minimum period of induction recommended for good quality edible oils; nevertheless, we consider that the C. brasiliense oil reached a long induction period of 8.   Moreover, we can consider that the prevention mechanism of oxidation through antioxidants occurred in the C. brasiliense pulp oil as its content of unsaturated FAs is higher than saturated (Table 2), which make the oil more susceptible to degradation [42,43]. The characterization of the profile of the FAs of C. brasiliense oil revealed a high content of monounsaturated fatty acids (MUFA), especially the oleic FA (56.61%), followed by the saturated FA palmitic (37.78%) and polyunsaturated linoleic acid (3.9%). The values we found are similar to those reported by Nascimento-Silva et al. (2019) [23]: 55.87%, 35.17%, and 1.53%, respectively, except linoleic FA that has a higher percentage in their study. Similar content (1.36%) was also reported by Roll et al. (2018) [19]. The results are expressed as the means ± mean standard error.
The oxidative stability, the parameters of quality within recommendations for edible vegetable oils, and the prevalence of unsaturated FAs are the factors that determine whether an oil is adequate for human consumption. Moreover, oils with a high level of MUFAs and PUFAs can improve the levels of serum lipids [44]. After 90 days of supplementation, we observed better levels of total cholesterol, LDL-c, and non-HDL-c (Table 3) in the groups supplemented with C. brasiliense oil as a lipidic source. The presence of PUFAs can explain the reduced plasmatic levels of total cholesterol as they act as a protective factor in the homeostasis of cholesterol due to the high number of unsaturations and thus less phospholipid-cholesterol interaction [45].
Another study states that diets rich in MUFAs can reduce the levels of total cholesterol total and LDL-c [46]. This could be one of the factors contributing to the low levels of LDL-c and non-HDL-c we found, despite high levels of SFA associated with the high plasmatic levels of LDL-c [47]. The group supplemented with olive oil in higher dosage (2000 mg/kg) also showed values significantly reduced, possibly due to the higher concentration of MUFAs present in this oil, as well as in C. brasiliense oil, which are mainly composed of oleic acid, followed by palmitic and linoleic acid [48]. We point out that the supplementation with C. brasiliense oil (2000 mg/kg) reached a better response in the parameters that are commonly associated with atherosclerosis (LDL-c and non-HDL-c) [49] when compared with groups CG and OO1, supplemented with soybean and olive oils, respectively.
Regard to body weight gain and adiposity index (Table 4), we noticed that animals kept a pattern of weight not differing statistically (p ≤ 0.05). Nevertheless, the group receiving higher doses of MUFA (OO + C. brasiliense oil) presented the lowest mean weight. Similar results we observed in adipose tissue weight (Table 4), the group supplemented with olive oil plus C. brasiliense oil had lower weight of the principal visceral fats, including epididymal adipose tissue; in mice, this is one of the main deposit areas of visceral fat [50]. Other relevant sites, such as mesenteric and retroperitoneal fat [51], also diminished compared with the control group.  This effect can be associated with the presence of tocopherols and mainly of tocotrienols that are present in both C. brasiliense oil, as demonstrated in our study, and olive oil [52]. Another work pointed out that the ingestion of gamma-tocotrienol (60 mg/kg/day) was capable of reducing the fat mass induced by different doses of glucocorticoids. Uto-Kondo et al. (2009) [53] evaluated the effect of a palm oil fraction rich in tocotrienol on the differentiation of adipocyte into 3T3-L1 cells and found that this antioxidant suppressed the differentiation of pre-adipocytes into adipocytes, potentially reducing weight gain.
Among the possible alterations in the liver (Table 5), we did not identify statistical difference regarding the presence of hepatic steatosis (p = 0.17) and microvesicular steatosis (p = 0.45). However, a build-up of free FAs occurred in the liver, except group OO + CO that showed only microvesicular steatosis, demonstrating that the synergic effect of the mix of olive oil with C. brasiliense oil may have played a slight protective role, due to a higher concentration of antioxidants. These are known for beneficial action in biological systems and protection against oxidative damages [54] as the oxidative stress is one of the causes of hepatocellular lesions [51].  CG indicates control group, supplemented with soybean oil (1000 mg/kg); OO1 and OO2 groups receiving olive oil (1000 mg/kg and 2000 mg/kg, respectively); CO1 and CO2 groups supplemented with Caryocar brasiliense oil (1000 mg/kg and 2000 mg/kg, respectively); and OO + CO group receiving olive oil (1000 mg/kg) with C. brasiliense oil (1000 mg/kg). Data are presented in relative frequency (absolute frequency). p-value in the chi-square test. Due to the sample size, two categories were grouped as follows: A ≤ 5% and ≥5%; B = Absent and Present, * p ≤ 0.05.
We detected significant differences between groups regarding the presence of Mallory Hyaline, and in post-test it was significantly more present in animals of all groups compared with GC, as well as group OO + CO compared with OO1. Apoptosis was significantly more prevalent in group CO1 compared with CG and OO1, and no presence was recorded in OO1. Palmeira et al. (2015) [55] reported that the administration of C. brasiliense oil at 400 mg/kg in mice induced with diethylnitrosamine 10 µg/g reduced the development of preneoplastic lesions and hepatic adenoma. Another study on C. brasiliense nut oil found that it can attenuate the biochemical markers of hepatic lesion and inflammation [21].
Among the data obtained in the histological analyses of the pancreas (Table 6), we did not find an association between the presence of Langerhans Islets (p = 0.93) or inflammatory cells in all groups (p = 0.38). None of the samples analyzed in our study showed alterations in pancreatic acini.  The amount of consumed SFA influences the accumulation of free FAs in the liver and activation of inflammatory markers [56]. For the inflammatory response to start it needs proinflammatory cytokines and chemokines such as TNF-α, IL-6, and MCP-1 [57]. Figure 5 shows the levels of circulating inflammatory cytokines IL-6 (p = 0.944), MCP-1 6 (p = 0.640), TNF-α (p = 0.834), and anti-inflammatory IL-10 (p = 0.709), without significant difference between treatments. Recent studies reported that phytochemicals present in plants could inhibit the inflammation, reducing the production of macrophages, proinflammatory factors and also blocking inflammatory pathways that liberate cytokines [58,59]. In a study on rats utilizing supplementation with C. brasiliense nut oil at a concentration of 6 mL/kg, the results suggest that it attenuates the acute inflammatory response when induced by CCl 4 , modulating the circulating levels of leptin, IL-6, LTB4, and LTB-5 positively [21]. kinase 2-leading to the activation of the signal transducer and activator of transcription 3 (STAT3). In our study, IL-10 was more active in the group supplemented with olive oil at the lower dose (1000 mg/kg). Nevertheless, more research is necessary to elucidate the molecular action mechanisms of oleic acid [21], which is the major component in CO and OO, and the phytochemicals present in C. brasiliense oil, such as phenolics, carotenoids, tocopherols, and phytosterols, that constitute possible mediators of these effects.  The tested dosages of CO did not demonstrate a protective effect on those parameters when compared with the group supplemented with soybean oil (CG) and both doses of olive oil (OO1; OO2). The cytokine IL-10 is necessary to inhibit the synthesis of proinflammatory cytokines [56]. It can exert anti-inflammatory effects through signal transducer pathway of the Janus kinase (JAK) of activation 3 (JAK-STAT3), binding IL-10 to the receptor in the targeted cell membrane-tyrosine kinase 2-leading to the activation of the signal transducer and activator of transcription 3 (STAT3). In our study, IL-10 was more active in the group supplemented with olive oil at the lower dose (1000 mg/kg). Nevertheless, more research is necessary to elucidate the molecular action mechanisms of oleic acid [21], which is the major component in CO and OO, and the phytochemicals present in C. brasiliense oil, such as phenolics, carotenoids, tocopherols, and phytosterols, that constitute possible mediators of these effects.

Raw Material
Caryocar brasiliense Cambess. Pulp oil was provided by RTK ® Cosmética e Indústria de Alimentos Naturais (Brasília, Brazil) and it had been extracted and cold-pressed. The soybean oil and olive oil were acquired from local shops.

Quality and Identity of C. brasiliense Oil
We qualified the oil regard to indexes of acidity (method Ca 5a-40), peroxide (method Cd 8-53), refraction (method Cc 7-25), iodine (method Cd 1-25), and saponification (method Cd 3-25) [60]. All analyzes were performed in triplicate. The acidity index (AI) was determined with 2 g of sample by the addition of a solution of ether-alcohol, using an indicator (phenolphthalein) and titration with NaOH until appearing the light pink color; the results of acidity in oleic acid were expressed in milligrams of sodium hydroxide per gram. The determination of the peroxide index (PI) was performed with 5 g of sample, in a solution of acetic acid-chloroform and potassium iodide, with posterior rest in the dark, titration with sodium thiosulfate 0.01 N, and addition of soluble starch as an indicator for observation of color change; the results were expressed in milliequivalents of peroxide per 1000 g of sample. The refraction index (RI) was read after filtering the sample to remove any impurities and traces of moisture, using a refractometer Abbé calibrated with distilled water (refraction index 1.3330) at 20 • C, with temperature corrected to 40 • C. For the iodine index (II), approximately 0.25 g of the sample of C. brasiliense oil was placed in Erlenmeyers with carbon tetrachloride and Wijs solution. Sodium thiosulfate was used for titration until the color changed from dark blue to pinkish, and the results were indicated in grams of iodine absorbed per gram of sample. The saponification index (SI) was determined by the addition of KOH and phenolphthalein to 5 g of the samples, and titration with HCl until pink disappeared and the value was expressed as the number of milligrams of potassium hydroxide (KOH) required to saponify 1 g of the oil sample.

Color
We measured the oil color using a colorimeter (CM-2300d, Konica Minolta, Ramsey, NJ, EUA), expressed in the classification scale CIE-L*, a*, b*, where the values L* indicate the lightness, a* represents the red-green axis, and b* yellow-blue axis. From the obtained results, we determined the hue angle (h) and the chroma (C*).

Total Carotenoids
For analysis of carotenoids, we utilized procedures described by Maldonade et al. (2012) [61] with slight modifications. Samples of 5 g C. brasiliense oil were placed in Erlenmeyer of 125 mL, we then added 25 mL acetone, shaked the solution, and stored it for two hours in the fridge. After centrifugation at 4000 rpm and 4 • C, the carotenoids were extracted by partitioning of the sobrenatant in petroleum ether, with successive washings with distilled water, followed by saponification with NaOH 10% in methylic alcohol for 2 h in the dark. The samples were again centrifuged and extracted by partitioning in petroleum ether, washed with distilled water, and recovered in a volumetric balloon, after excess water removal with anhydrous sodium sulfate. We made readings of absorbance in a spectrophotometer at 450 nm for quantification of total carotenoids.

Optical Properties
The samples of C. brasiliense oil were diluted in hexane (spectroscopic grade 99.9%) at concentrations of 5 g/L. For optical measurements, we utilized a quartz cuvette with an optical path of 10 mm and four polished slides. We measured UV-Vis absorption using a spectrometer Lambda 265-Perkin Elmer ™ , Waltham, MA, USA and the absorption spectrum between 200 and 600 nm was collected. For the fluorescence map (excitation/emission), we used a bench-top spectrophotometer FS-2 (Scinco ™ , Seoul, Korea), the samples were excited from 200 to 400 nm every 5 nm, the measured emission being 250 and 600 nm with a 1 nm resolution. For all assessments, the excitation and emission slots were fixed in 5 nm. All optical measurements were performed at room temperature.

Oxidative Stability
The oxidative stability was analyzed by measuring the induction period using the Rancimat method. We utilized the equipment 893 Professional Biodiesel Rancimat (Metrohm ® , São Paulo, Brazil), where 3.0 g sample of the oil without dilution was put into a sealed glass reaction vessel and was submitted to 110 • C under constant air flux at 10 L h −1 , which passed through the samples and then into a measuring vessel containing 50 mL ultrapure water in which the conductivity generated by volatile products during the vegetable oil degradation was measured as a function of time, according to the European rule EN14112. The induction period was determined by the second derivative method of the conductivity curve.

Profile of Fatty Acids
The methylic esters of the fatty acids (FAME) were prepared according to the method of Maya and Rodriguez-Amaya with a solution of derivatization of ammonium chloride, methanol, and sulfuric acid. FAMEs were analyzed by gas chromatography (GC 2010, Shimadzu) to obtain their peaks. The equipment utilized a flame ionization detector (FID) and a capillary column (BPX-70, 0.25 m internal diameter, 30 mm long, and 0.25 mm thick). The temperature of the injector and the detector was 250 • C. The initial temperature of the column was 80 • C, which was held for 3 min and then increased at a rate of 10 • C min −1 until reaching 140 • C, followed by an increase to 240 • C at a rate of 5 • C min −1 for 5 min. We identified the individual peaks of FAME were identified comparing their relative retention time with the standard of 37 FAMEs (Supelco C22, 99 % pure).

Experimental Design
The project was carried out according to the ethic regulations and guidelines, and the experimental protocol approved by the Ethics Committee in Use of Animals (Protocol n. 954/2018). We utilized Swiss mice (Mus musculus), adult males, provided by the Central Biotherium/CCBS/UFMS, kept under temperature at 22 ± 2 • C, relative air humidity of 50-60%, with a light/dark cycle of 12 h, with standard diet AIN-93M and water ad libitum.

Biochemical Analysis
Blood samples were collected and centrifuged to obtain the serum fractions and determine the levels of triacylglycerols, total cholesterol, LDL-c, HDL-c, VLDL-c, non-HDL-c, and blood glucose. Intervention protocol of the supplementation with Caryocar brasiliense oil. CG indicates control group, supplemented with soybean oil (1000 mg/kg); OO1 and OO2 groups receiving olive oil (1000 mg/kg and 2000 mg/kg, respectively); CO1 and CO2 groups supplemented with C. brasiliense oil (1000 mg/kg and 2000 mg/kg, respectively); and OO + CO group receiving olive oil (1000 mg/kg) with C. brasiliense oil (1000 mg/kg).

Biochemical Analysis
Blood samples were collected and centrifuged to obtain the serum fractions and determine the levels of triacylglycerols, total cholesterol, LDL-c, HDL-c, VLDL-c, non-HDL-c, and blood glucose. The parameters were determined by the enzymatic-colorimetric method, according to the manufacturer's instructions (Labtest™, Lagoa Santa, Minas Gerais, Brazil).

Body Weight, Visceral Fat, and Liver Weight
The bodyweight of the animals was recorded weekly on a semi-analytic balance (Bel Diagnóstica ® ), expressed in grams. The liver and the sites of omental, epididymal, retroperitoneal, perirenal, and mesenteric fat of each animal were removed and weighed on a semi-analytic balance (Bel Diagnóstica ® ) for comparison between the studied groups. The adiposity index (AI) was calculated according to Equation (1) [62].

Histopathological Analysis
Samples of liver and pancreas were fixed with 10% formalin solution. After fixation, the specimens were dehydrated, embedded in paraffin, cut in a microtome to a thickness of 5 mm each, and stained with hematoxylin and eosin [63,64].
We evaluated the architecture of the pancreas according to alterations in the Langerhans islets (without alteration, discrete atrophy, atrophy, discrete hypertrophy, and hypertrophy), pancreatic acini (without alteration, necrosis/atrophy), and inflammation by the presence of inflammatory cells inside (insulitis) and on the periphery (perinsulitis) in the Langerhans islets [65][66][67].

Quantification of Cytokines
The serum was collected after centrifugation and stored again at −80 • C until cytokine analysis, according to the recommendations of the manufacturer (MILLIPLEX MAP/Mouse Cytokine/Chemokine and Adipocyte Magnetic Bead panel) (Millipore, Billerica, MA, USA). The concentrations of the following cytokines were analyzed: IL-10, IL-6, MCP-1, and TNF-α using the MCYTOMAG-70K kit, in MAGPIX ™ with xPONENT software. The concentration of the cytokines IL-10, IL-6, MCP-1, and TNF-α in the serum was expressed as cytokine picograms related to protein content (mg of protein).

Statistical Analyses
The results were expressed as mean ± standard error. For multiple comparisons of parametric results, we performed an ANOVA followed by a Tukey post-test. The chi-square test was applied to evaluate associations in histological analyses, followed by a Bonferroni test. A significance level of p < 0.05 was adopted. For statistical analyses we used the software Jandel Sigma Stat, version 3.5 (Systat software, Incs., San Jose, CA, USA), and Bioestat 5.0 (Systat software, Incs., San Jose, CA, USA).

Conclusions
Caryocar brasiliense oil can be considered an oil with adequate quality for human consumption, with excellent oxidative stability and shelf-life, having the potential to be commercialized at large-scale in the food market. The C. brasiliense oil promotes beneficial effects on biochemical parameters, mainly in the lipidic profile, reducing indices associated with inflammatory processes. Combined with olive oil, C. brasiliense oil reduces the development of white adipose tissue, possibly due to the presence of antioxidants, such as tocopherols and tocotrienols.