Larvicidal Enzyme Inhibition and Repellent Activity of Red Mangrove Rhizophora mucronata (Lam.) Leaf Extracts and Their Biomolecules against Three Medically Challenging Arthropod Vectors

The larvicidal potential of crude leaf extracts of Rhizophora mucronata, the red mangrove, using diverse solvent extracts of the plant against the early fourth instar larvae of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti mosquito vectors was analyzed. The acetone extract of R. mucronata showed the greatest efficacy: for Cx. quinquefasciatus (LC50 = 0.13 mg/mL; LC90 = 2.84 mg/mL), An. stephensi (LC50 = 0.34 mg/mL; LC90 = 6.03 mg/mL), and Ae. aegypti (LC50 = 0.11 mg/mL; LC90 = 1.35 mg/mL). The acetone extract was further fractionated into four fractions and tested for its larvicidal activity. Fraction 3 showed stronger larvicidal activity against all the three mosquito larvae. Chemical characterization of the acetone extract displayed the existence of several identifiable compounds like phytol, 3,7,11,15-tetramethyl-2-hexadecen-1-ol, 1-hexyl-2-nitrocyclohexane, eicosanoic acid etc. Enzyme assay displayed that R. mucronata active F3-fractions exert divergent effects on all three mosquitos’ biochemical defensive mechanisms. The plant fractions displayed significant repellent activity against all the three mosquito vectors up to the maximum repellent time of 210 min. Thus, the bioactive molecules in the acetone extract of R. murconata leaves showed significant larvicidal and enzyme inhibitory activity and displayed novel eco-friendly tool for mosquito control.


Mortality Bioassays of Acetone Extract Fractions
The efficacy of plant extracts varies from species to species and plant parts [16]. The variability in toxic concentrations of the various plant extracts, i.e., to achieve mosquitocidal activity, may be due to differences in concentration levels between the insecticidal components of each plant; moreover, the effect of each plant extract will vary with the time of collection and season [13]. It has been reported [15] that the seed methanol extract of Clitoria ternatea was effective against An. stephensi, Ae. aegypti, and Cx. quinquefasciatus larvae with LC 50 values of 65.2, 154.5, and 54.4 ppm, respectively. In our study, the acetone extracts of R. mucronata leaves were fractioned and four different fractions were collected. These four fractions were tested for larvicidal activity against three important mosquitoes in public health terms (Table 2). When testing Fraction 3, we observed the greatest larvicidal activity against Ae. aegypti (LC 50 = 0.1037; LC 90 = 1.0025 mg/mL), An. stephensi (LC 50 = 0.1480; LC 90 = 4.6480 mg/mL) and Cx. quinquefasciatus (LC 50 = 0.174348; LC 90 = 16.73929 mg/mL), respectively. Earlier studies reported that methanolic extracts of plants are larvicidal against An. stephensi and Cx. quinquefasciatus [16,17]. Kamaraj and colleagues reported the highest larval mortality in leaf petroleum ether and flower methanol extracts of Cryptocoryne auriculata, flower methanol extracts of Leucas aspera, leaf and seed methanol extracts of Solanum torvum, and leaf hexane extracts of Vitex negundo against An. subpictus larvae (LC 50 = 44. 21 . Ansari and colleagues examined the larvicidal activity of Pinus longifolia oil against three vector mosquitoes, namely, Aedes aegypti (LC 50 = 82.1 ppm), Cx. quinquefasciatus (LC 50 = 85.7 ppm) and An. stephensi (LC 50 = 112.6 ppm).

Chemical Characterization of R. mucronata Extract
GC-MS analysis of acetone solvent extracts of R. mucronata leaves (Figure 1) showed 13 peaks representing 13 compounds. The 13 compounds were characterized and identified as shown in Table 3. phytol, 3,7,11,15-tetramethyl-2-hexadecen-1-ol, 1-hexyl-2-nitrocyclohexane, eicosanoic acid, estra-1,3,5(10)-trien-17, betaol, sulfurous acid, octadecyl 2-propyl ester, 2-heptadecenal, 1-hexyl-2nitrocyclohexane, 17-pentatriacontene, tritetracontane, urs-12-en-28-ol and squalene. The peak area percentage was prominent in Eicosanoic Acid (38.24%) with retention time at 19.205. Since the Peak area percentage was prominent in Eicosanoic Acid and this might have played a key role in mosquitocidal activity against all the three arthropod vectors. Preliminary phytochemical screening of whole plant extracts revealed the presence of phenol, flavonoids, alkaloids, saponins, tannins, glycosides, amino acids, quinones and carbohydrates in the plant extracts (Table 4).  Plant-based compounds are known to be eco-friendly and can be used in mosquito larval control safely. Furthermore, these natural products are readily biodegradable and safe to other organisms [18]. The potent larvicidal activity of R. murconata could be attributed to the presence of tannins, phenols, flavonoids, saponins, glycosides and quinones ( Figure 2). The isolation of these compounds, as defined here, could provide the basis for developing natural mosquitocidal products as a substitute for synthetic insecticides-or for developing mosquito repellents. The fractions obtained from the Adhatoda vasica were an effective larvicidal agent against the Cx. quinquefasciatus and Ae. aegypti larvae [19]. It was highly toxic to mosquito larvae and also inhibited the development of pupae. The high rate of larval mortality of mosquitoes observed at higher concentrations (250 ppm of A. vasica) within a 24 h exposure indicates the high toxicity of the product. The plants tested in the present study are reported to be eco-friendly and toxic to agriculturally important insect pest [20]. For example, essential oils from Citronella and Eucalyptus are often used in repellents sold under several brand names [21]. Recent studies have indicated that phytol, a precursor of synthetic vitamin E and vitamin K (which was identified in our analysis as well), exhibits antioxidant and antinociceptive effects and was cytotoxic against the MCF−7 breast cancer cell line [22].
The FT-IR spectrum analysis of acetone solvent extract of R. mucronata ( Figure 3) showed 13 peaks indicating the presence of functional groups. The functional groups of the detected peak values were identified (Table 5). Alkane, Amine amd Alkyl were invariably present in acetone extracts. The column separation was eluted the different fractions of R. mucronata. Besides the active fraction of F3 was compared with a standard of squalene. The retention time of fraction F3 got eluted in 4.8 min at 220 nm and it was comparable to the standard peak of squalene ( Figure 4). Correspondingly, Octacosane, derived from Couroupita guianensis, showed the highest percentage, at 31.86% peak area in the active fraction F6 and was previously shown to have toxicity to early third instars of Cx. quinquefasciatus (Say.) [23]. Octacosane was also shown to be present in the unsaponifiable phase of common chicory, Cichorium intybus L. at 1.34% peak area, exhibiting larvicidal activity against Anopheles pharoensis (Theobald) with LC 50 value of 13.62 mg/kg_1 [23]. Spirostan-3,15-diol,3-(4-methylbenzenesulfo-nate) at 5.20% of peak area, was isolated by Soule et al. [24] as a spirostane glycoside from the Solanum laxum (Steud.) aerial parts were reported to have toxicity against the aphid, Schizaphisgra minum (Rondani) [25]. FractionF6 also contains hexacosane at 17.54% peak area. Hexacosane, in the leaf oil of Solanum sarrachoides (Sendt.), exhibits oviposition deterrence against red spidermites, (Tetranychus evansi) [26,27]. The sensitivity of adults of Ae. aegypti has been shown as resulting from the presence of 1,8-cineole, α-pinene and p-cymene and is correlated to the amount of 1,8-cineole in the tested extract [28]. The insecticidal activity of the essential oil of Eucalyptus tereticornis observed in the current work, might be explained by the presence of one of its major components (p-cymene) but also by a minor compound (1,8-cineole) in our extracts, which both have demonstrated insecticidal activity against An. gambiae [27].
Plant-based compounds are known to be eco-friendly and can be used in mosquito larval control safely. Furthermore, these natural products are readily biodegradable and safe to other organisms [18]. The potent larvicidal activity of R. murconata could be attributed to the presence of tannins, phenols, flavonoids, saponins, glycosides and quinones (Figure 2). The isolation of these compounds, as defined here, could provide the basis for developing natural mosquitocidal products as a substitute for synthetic insecticides-or for developing mosquito repellents. The fractions obtained from the Adhatoda vasica were an effective larvicidal agent against the Cx. quinquefasciatus and Ae. aegypti larvae [19]. It was highly toxic to mosquito larvae and also inhibited the development of pupae. The high rate of larval mortality of mosquitoes observed at higher concentrations (250 ppm of A. vasica) within a 24 h exposure indicates the high toxicity of the product. The plants tested in the present study are reported to be eco-friendly and toxic to agriculturally important insect pest [20]. For example, essential oils from Citronella and Eucalyptus are often used in repellents sold under several brand names [21]. Recent studies have indicated that phytol, a precursor of synthetic vitamin E and vitamin K (which was identified in our analysis as well), exhibits antioxidant and antinociceptive effects and was cytotoxic against the MCF−7 breast cancer cell line [22].  (Table 5). Alkane, Amine amd Alkyl were invariably present in acetone extracts. The column separation was eluted the different fractions of R. mucronata. Besides the active Molecules 2020, 25, x FOR PEER REVIEW 9 of 18 fraction of F3 was compared with a standard of squalene. The retention time of fraction F3 got eluted in 4.8 min at 220 nm and it was comparable to the standard peak of squalene ( Figure 4). Correspondingly, Octacosane, derived from Couroupita guianensis, showed the highest percentage, at 31.86% peak area in the active fraction F6 and was previously shown to have toxicity to early third instars of Cx. quinquefasciatus (Say.) [23]. Octacosane was also shown to be present in the unsaponifiable phase of common chicory, Cichorium intybus L. at 1.34% peak area, exhibiting larvicidal activity against Anopheles pharoensis (Theobald) with LC50 value of 13.62 mg/kg_1 [23]. Spirostan-3,15-diol,3-(4-methylbenzenesulfo-nate) at 5.20% of peak area, was isolated by Soule et al. [24] as a spirostane glycoside from the Solanum laxum (Steud.) aerial parts were reported to have toxicity against the aphid, Schizaphisgra minum (Rondani) [25]. FractionF6 also contains hexacosane at 17.54% peak area. Hexacosane, in the leaf oil of Solanum sarrachoides (Sendt.), exhibits oviposition deterrence against red spidermites, (Tetranychus evansi) [26,27]. The sensitivity of adults of Ae. aegypti has been shown as resulting from the presence of 1,8-cineole, α-pinene and p-cymene and is correlated to the amount of 1,8-cineole in the tested extract [28]. The insecticidal activity of the essential oil of Eucalyptus tereticornis observed in the current work, might be explained by the presence of one of its major components (p-cymene) but also by a minor compound (1,8-cineole) in our extracts, which both have demonstrated insecticidal activity against An. gambiae [27].

Enzyme Assay
The active acetone extract F3-fractions of R. murconata inhibited α-carboxylesterase activity of fourth instar larvae tested at 24 h. The activity decreased in dose dependent manner across Cx. In contrast to the above enzyme levels, GST and CYP450 enzyme regulations upregulated significantly in dose dependent manner. The enzyme ratio increased significantly initially in the lower doses (0.06 mg/mL) and relics constant at the higher sub-lethal dosages across all the mosquito vectors. Besides, the GST ( Figure 5J-L) and CYP450 ( Figure 5M-O) enzyme regulations uplifted significantly in Cx. quinquefasciatus (F4,20 = 11.21; p < 0.0001) as compared to Ae. aegypti (F4,20 = 16.71; p < 0.0001) and An. stephensi (F4,20 = 14.55; p < 0.0001). Our enzyme analysis data was interpreted to display that R. mucronata active F3-fractions exert divergent effects on all three mosquitos' biochemical defensive mechanisms.
In addition to their potential for mosquito control, the R. mucronata extracts may exhibit enzyme inhibitory activity at currently unknown bioactivities and further studies are required to investigate the possibility of the herein identified constituents of R. mucronata extracts for the development of environmentally-friendly applications. Indeed, phytochemicals from plant sources may also act as insect growth regulators, repellents, ovipositor attractants, among others [26,27].

Repellent Activity
The active fractions of R. mucronata showed significant repellent activity against Cx. quinquefasciatus In contrast to the above enzyme levels, GST and CYP450 enzyme regulations upregulated significantly in dose dependent manner. The enzyme ratio increased significantly initially in the lower doses (0.06 mg/mL) and relics constant at the higher sub-lethal dosages across all the mosquito vectors. Besides, the GST ( Figure 5J-L) and CYP450 ( Figure 5M-O) enzyme regulations uplifted significantly in Cx. quinquefasciatus (F 4,20 = 11.21; p < 0.0001) as compared to Ae. aegypti (F 4,20 = 16.71; p < 0.0001) and An. stephensi (F 4,20 = 14.55; p < 0.0001). Our enzyme analysis data was interpreted to display that R. mucronata active F3-fractions exert divergent effects on all three mosquitos' biochemical defensive mechanisms.
In addition to their potential for mosquito control, the R. mucronata extracts may exhibit enzyme inhibitory activity at currently unknown bioactivities and further studies are required to investigate the possibility of the herein identified constituents of R. mucronata extracts for the development of environmentally-friendly applications. Indeed, phytochemicals from plant sources may also act as insect growth regulators, repellents, ovipositor attractants, among others [26,27].

Repellent Activity
The active fractions of R. mucronata showed significant repellent activity against Cx. quinquefasciatus  . Mean (± SEM) followed by the same letter in the above bars indicate no significant difference (p < 0.05) in a Tukey's test.

Crude Extract Preparation
Collected fresh leaves were air dried under shadow at room temperature for 7-10 days. The leaves (250 g) were dried and mechanically powdered using a mixer and crushed (Mixer Grinders Stylo 750) to well particle size. Leaf powdered extracts of R. mucronata (250 g) was prepared by using soxhlet device utilizing diverse solvents including acetone, ethyl acetate, petroleum benzene, and methanol. Further the solvents were evaporated by using rotary evaporator and the remaining were preserved under 4 °C for further assays. The total yields were observed 2.15, 1.76, 1.95 and 2.02 g, respectively.

Mosquitoes
Ae. aegypti, An. stephensi, and Cx. quinquefasciatus larvae were collected from the local areas of Pallipalayam (Latitude: 11.3450° N; Longitude: 77.7309° E), Tiruchengode, Tamil Nadu, India. Furthermore, the culture were maintained in the laboratory were kept in sterile vessels filled with water, maintained at 28 ± 2 °C and 75%-80% relative humidity (RH) under a fixed photoperiod A B C Figure 6. Repellency of acetone extract F3-fractions of R. murconata against Cx. Quinquefasciatus (A), Ae. Aegypti (B), and An. Stephensi (C). Mean (± SEM) followed by the same letter in the above bars indicate no significant difference (p < 0.05) in a Tukey's test.

Crude Extract Preparation
Collected fresh leaves were air dried under shadow at room temperature for 7-10 days. The leaves (250 g) were dried and mechanically powdered using a mixer and crushed (Mixer Grinders Stylo 750) to well particle size. Leaf powdered extracts of R. mucronata (250 g) was prepared by using soxhlet device utilizing diverse solvents including acetone, ethyl acetate, petroleum benzene, and methanol. Further the solvents were evaporated by using rotary evaporator and the remaining were preserved under 4 • C for further assays. The total yields were observed 2.15, 1.76, 1.95 and 2.02 g, respectively.

Mosquitoes
Ae. aegypti, An. stephensi, and Cx. quinquefasciatus larvae were collected from the local areas of Pallipalayam (Latitude: 11.3450 • N; Longitude: 77.7309 • E), Tiruchengode, Tamil Nadu, India. Furthermore, the culture were maintained in the laboratory were kept in sterile vessels filled with water, maintained at 28 ± 2 • C and 75%-80% relative humidity (RH) under a fixed photoperiod (14:10 L/D). The emerged adult mosquitoes were maintained under the same conditions as the larvae.

Larval Mortality Assay
Larval bioassays were executed on the fourth instars of Cx. quinquefasciatus, An. stephensi and Ae. aegypti with diversified dosages (0.5, 0.10, 0.15, 0.20 and 0.25 mg/mL) of R. mucronata leaf extracts. Minimal of 20 larvae/each concentration were utilized for all the assays, and the procedure were three times replicated. The lethal dosage (LC 50 and LC 90 ) was calculated based on Probit analysis [30]. Twenty larvae of fourth instars were presented to a 200 mL glass jar supplemented with discriminating dosage of leaf extracts along with 50 mg/L of yeast extract. A group of control was also kept alone treated with methanol. Three times were replicated along with control in each replication. Mortality percentage in the treatments was corrected whenever required using Abbott formula [31].

Preparation of Whole-Body Homogenates for Enzyme Assay
The control and plant extract exposed fourth instar larvae were rinsed with sterile distilled water, and the adhering water was distant by using blotting tissue paper from the surface. The larval homogenized were distinct using a handy homogenizer in Eppendorf tubes containing ice-cold sodium phosphate buffer (500 µL−20 mM, pH 7.0). Homogenates were further spin for 20 min at 8000× g at 4 • C) and the supernatants separated were utilized for enzyme experiments. The homogenates separated were reserved at −4 • C for further experiments.

Carboxyl Esterase Assays
The αand βcarboxylesterase activity was estimated using the larval extracts in phosphate potassium buffer (0.2 M; pH = 7.1) were set to (20 µL; 84 µg protein). Further, it was assorted with 500 µL buffer (0.3 mMα-or β-naphthyl acetate in 0.1 M phosphate potassium at pH 7.2 containing 1% acetone). Enzyme activity of one unit was definite as the enzyme amount essential to generate 1 µmol of αor β-naphthol/minute.

Superoxide Dismutase Activity
The Superoxide Dismutase (SOD) assays were carried out by using the Superoxide dismutase determination kit (Sigma-Aldrich, Bangalore, India). Activity of SOD per 1 unit was resolute as the required enzyme to slab the increment absorbance by 50% at 440 nm.

Glutathione-S-Transferase Activity
A total of 250 µL of fourth instars were homogenized in sodium phosphate solution (50 mM; pH = 7.2) and spin at 10,000× g at 4 • C for 20 min. The Glutathione-S-Transferase (GST) assay Kit (Sigma-Aldrich, Catalog 0410, Bangalore, India) was utilized to investigate the conjugation of the thiol group of glutathione to the 1-chloro-2,4-dinotrobenzene (CDNB) substrate. Further assays were performed based on the standard protocol prescribed in the Kit. The GST activity was stated as µmol/mg protein/min substrate conjugated.

Cytochrome P450 Activity
Fourth instar visible to bioactive fractions3 were rinsed in sterile water and separated. Further the larvae were kept in 40 mM sodium phosphate solution with maintained pH: 7.2 and cooled earlier separation. The abdominal segments, heads, and digestive tissues were extracted. For determining the enzyme activity, carcasses were taken. The enzymes were measured by using ethoxycoumarin-O-deethylase existing in the body walls and it was stated as mol 7-OH/mg larvae/min.

Phytochemical Analysis
Chemical testing for the presence of carbohydrates, alkaloids, saponins, phenolics, tannins, terpenes and flavonoids was evaluated in the mangrove extracts and the most active extract using the standard procedure of Harborne [32].

Phenols: Ferric Chloride Test
Extracts were treated with 3-4 drops of ferric chloride solution. Formation of bluish black color indicates the presence of phenols.

Flavonoids Test
Ammonia solution (5 mL) was added to a portion of the crude extract followed by addition of concentrated H 2 SO 4 . Formation of a yellow coloration in the extract indicates the presence of flavonoids. The yellow coloration disappears after some time.

Alkaloids: Wagner Reagent
Extracts were dissolved individually in dilute Hydrochloric acid and filtered. Filtrates were treated with Wagner's reagent (1.27 g Iodine in 2 g of Potassium Iodide). The formation of a brown/reddish precipitate indicates the presence of alkaloids.

Saponin
Half mg of the extract was shaken with 2 mL of distilled water. If foam produced persists for ten min, it indicates the presence of saponins.

Tannin Test: Gelatin Test
To the extract, 1% gelatin solution containing sodium chloride was added. Formation of white precipitate indicates the presence of tannins.

Glycosides Test
Minimum quantities of the extracts were hydrolyzed with hydrochloric acid for a few minutes on a water bath and the hydrolysate was subjected to the following tests.
The extracts were treated with chloroform and evaporate it to dryness. Separately, 0.4 mL of glacial acetic acid containing a trace amount of ferric chloride was added and transferred to a small test tube added with carefully 0.5 mL of concentrated sulfuric acid by the side of the test tube; blue color appeared in the acetic acid layer, indicating the presence of glycosides.

Ninhydrin Test
To the extract, 0.25% w/v Ninhydrin reagent was added and boiled for few minutes. Formation of blue or blue to violet indicates the presence of amino acid.

Benedict Test
Two mL of crude extracts were dissolved individually in 5 mL distilled water and filtered. Filtrates were treated with Benedict's reagent and heated gently. Orange-red precipitate indicates the presence of reducing sugars.

Starch Test
To 5 mL of the extract, a few drops of iodine was added. The presence of starch was indicated by the formation of blue color.

Flavonoids Test
Ammonia solution (5 mL) was added to a portion of the crude extract followed by addition of concentrated H 2 SO 4 . Formation of a yellow coloration in the extract indicates the presence of flavonoids. The yellow coloration disappears after some time.

Repellent Assay
The active fractions F3 derived from R. mucronata extracts evaluated for its repellency against all the three mosquito vectors with sub-lethal dosages (0.06, 0.08, 0.09 and 0.1 mg/mL). Further repellent assay experiments were adapted from our previous research protocol Thanigaivel et al. [29].

GC-MS Analysis and Compound Identification
Among the different extracts (acetone, petroleum benzene, ethyl acetate and methanol) which were tested for their larvicidal toxic, and based on our preliminary tests, the highest toxicity was observed in acetone extracts of R. mucronata. Therefore, the acetone extract was used for further experiments. The isolated R. mucronata acetone extract was dissolved in (1:1) ratio with ethyl alcohol. From this, 2 µL of crude solution was dissolved in HPLC-grade methanol and subjected to GC and MS JEOL GC (Agilent Technologies 6890N, PerkinElmer, Bangalore, India) mate equipped with secondary electron multiplier. The downstream procedure of chemical characterization was carried out by our previous methodology (Agilent Technologies 6890N, PerkinElmer, Bangalore, India) [29]. The molecular weight, molecular formula and structure of the compounds of tested materials were ascertained by interpretation on mass spectrum of GC-MS using the database of the National Institute Standard and Technology (NIST).

FT-IR Analysis
Dried acetone extract was used for FT-IR analysis according to the combined methods reported in [33,34]. A total of 2 mg sample were mixed with 100 mg KBr (FT-IR grade) and then compressed to prepare a translucent sample disc (3 mm diameter), which was immediately kept in the sample holder. The sample was scanned and the FT-IR spectra recorded in the absorption range of 400 and 4000 cm −1 . FT-IR analysis was performed using a Perkin-Elmer spectrophotometer (Perkin-Elmer FT-IR, Spectrum 2 Singapore, L160000A) to detect characteristic peaks, types of chemical bonds, and probable functional groups present in the sample. FT-IR peak values were recorded and the analysis was repeated twice for spectrum confirmation.

High Performance Liquid Chromatography (HPLC) Analysis
The HPLC (Flexar HPL, Perkin Elmer, Chennai, India) analysis was carried out with a C18 column (220 nm) (250 × 4.6 mm). Acetonitrile and water were used as mobile phase at flow rate of 1 mL/min. Peaks obtained were detected at 220 nm. Fraction F3 was analyzed and compared with a purchased standard of squalene (Sigma-Aldrich, Analytical Standard). The yield of the collected fraction dry weight is 0.57 mg.

Statistical Analysis
Average larval mortality data were analyzed by probit analysis to calculate the LC 50 , LC 90 , and other statistics with 95% confidence intervals of upper confidence limit (UCL) and lower confidence limit (LCL) standards and the chi-squared test. SPSS 14.0 (IBM Inc., Chicago, IL, USA) was used to analyze the data.

Conclusions
As an endnote, the phyto-chemical characterization of acetone extracts of R. mucronata through GC-MS analysis displayed 13 major phyto-chemicals and the individual bio-active fractions were purified and characterized through HPLC and FT-IR assays which signified that active fraction-F3 was prominent as compared to other fractions and the larvicidal activity of active fraction F3 delivers that they are lethal against larvae of all three mosquito vectors. Moreover, the enzyme assays signify that the acetone extract F3-fractions significantly drifted the enzyme pattern in all five major digestive and detoxifying enzymes. Furthermore, the sub-lethal dosage of active fraction F3 also showed significant repellent activity in a dose dependent manner across all the three arthropod vectors. From this research, it is suggested that the mangrove leaf extract of R. murconata contains several bioactive molecules that might be useful as eco-friendly larvicides and repellent for managing blood sucking pests of medical importance. Further functional and mechanistic studies on how these compounds exert larvicidal activity may pave the way for environmentally safe botanical insecticides to control mosquito populations.
Author Contributions: S.K., M.V., K.U., V.M. and P.K. designed the research plan and drafted the manuscript. M.V., K.U., V.M., P.V.-S., K.C., performed the experimental works and data compilation. M.V., K.U., P.K., and S.K. coordinated the work and discussed the results. S.K. and P.K. revise the manuscript. All authors read and approved the final manuscript.
Funding: This research was partially funded by Post-Doctoral Program, Chiang Mai University, Thailand.