Antiparasitic Constituents of Beilschmiedia louisii and Beilschmiedia obscura and Some Semisynthetic Derivatives (Lauraceae)

The MeOH/CH2Cl2 (1:1) extracts of the roots and leaves of Beilschmiedia louisii and B. obscura showed potent antitrypanosomal activity during preliminary screening on Trypanosoma brucei brucei. Phytochemical investigation of these extracts led to the isolation of a mixture of two new endiandric acid derivatives beilschmiedol B (1) and beilschmiedol C (2), and one new phenylalkene obscurene A (3) together with twelve known compounds (4–15). In addition, four new derivatives (11a–11d) were synthesized from compound 11. Their structures were elucidated based on their NMR and MS data. Compounds 5, 6, and 7 were isolated for the first time from the Beilschmiedia genus. Additionally, the NMR data of compound 4 are given here for the first time. The isolates were evaluated for their antitrypanosomal and antimalarial activities against Tb brucei and the Plasmodium falciparum chloroquine-resistant strain Pf3D7 in vitro, respectively. From the tested compounds, the mixture of new compounds 1 and 2 exhibited the most potent antitrypanosomal activity in vitro with IC50 value of 4.91 μM.


Introduction
The genus Beilschmiedia is one of the most widespread genera of the Lauraceae family. It is commonly distributed in tropical Asia and Africa and comprises of about 250 species. Forty-one of these species can be found in Cameroon, principally, in the South Western, Center; Eastern, Littoral, and Western regions [1,2]. Plants of the Beilschmiedia genus are largely used in traditional medicine in developing countries for the treatment of many ailments. The bark and leaves of some Beilschmiedia species have been reported to be used in traditional medicine for the treatment of uterine tumors,
The derivatization of compound 11b with thiosemicarbazide reagent [23] led to compound 11c (Scheme 1) as a white amorphous powder. The molecular formula of 11c, C 23 H 35 N 3 S, with eight degrees of unsaturation, was deduced from its NMR data and the high-resolution electrospray ionization (HRESIMS; Figure S10), which showed the protonated molecular ion peak [M + H] + at m/z 386.2658 (calcd for C 23 H 34 N 3 S, 386.2624). The 1 H and 13 C-NMR data of the two compounds 11c and 11b (Table 2; Figures S11 and S12) were close to each other except some remarkable discrepancies. The main discrepancy observed in the 13 C-NMR spectrum of 11c was the signal of a conjugated imine at δ C 145.5 (C-1") in replacement of the aldehyde carbonyl group at δ C 193.9 (C-1") in 11b. In addition, the 13 C-NMR spectrum showed the signal of the thiocarbonyl carbon resonance at 180.4 (C-2"), the shielded ∆ 5,6 carbon resonances at δ C 139.7 (C-6) and 139.5 (C-5). Discrepancies were also observed on the 1 H-NMR spectrum, where the aldehyde proton at δ H 9.46 (1H, s, H-1") in 11b was replaced by four other proton signals at δ H 12.43 (1H, s, NH-1), 9.63 (1H, s, NH 2 -1), 8.39 (1H, s, H-1"), and 8.01 (1H, s, NH 2 -2). Based on the above evidences, compound 11c was determined as a new beilschmiedic acid (D) derivative to which the trivial name beilschmiecarbazone (Figure 1) was given.
Molecules 2020, 25, x 6 of 16 Molecules 2020, 25, x; doi: www.mdpi.com/journal/molecules 3.85 (3H, s, 4′′′-OMe). Based on the above data, compound 11d was confirmed as a new derivative of beilschmiedic acid E (11) trivially named beilschmiegallate ( Figure 1). The mixture of compounds 1 and 2 was obtained as a yellowish oil with the same Rf on the thin layer chromatography (TLC) in different systems. Its HRESIMS spectra ( Figure S20) showed the potassium adducts peak [M + K] + at m/z 367.2303 (calcd for C23H37KO, 367.2398). The combination of this data with those of the 1 H and 13 C NMR spectra clearly indicated that the yellowish sample was the mixture of two isomers. In fact, the 13 C NMR spectrum ( Figure S22) supported by DEPT and HSQC ( Figure S24) data displayed 34 carbon signals including one quaternary olefinic carbon at δC 141.6 (C-6, 2), twenty-two methine carbons (including seven olefinic methine carbons at δC 126.0 (C-5, 2), 126.1 (C-8, 2), 127.9 (C-5, 1), 128.2 (C-9, 2), 128.9 (C-9, 1), 131.3 (C-8, 1), and 134.2 (C-4, 1)), twenty-one methylene carbons (including two oxymethylenes at δC 65.5 (C-1′′, 2) and 67.6 (C-1′′, 1)), and methyl carbon signal at δC 14.3. The 13 C NMR spectrum of 1 and 2 (Table 1) also exhibited characteristic signals for molecules containing an aliphatic long chain moiety at δC 29.9-29.5. In addition, the 13 C NMR spectrum of the mixture exhibited characteristic carbon signals for two tetracyclic endiandric acid skeletons where, one contains an ∆ 4,5 and ∆ 8,9 and the other an ∆ 5,6 and ∆ 8,9 carbon-carbon double bond equivalents [4,[13][14][15]20,21]. These observations were confirmed from the fact that carbon resonances of one of the constituents (1) were nearly similar to those of compound 11a. The main dissimilarity between these compounds was at the level of their masses, which differ by 14 amu. The 1 H NMR spectrum (Table 1; Figure S21) of the mixture showed signals of seven olefinic proton resonances between δH 5.42-6.10, confirming the presence of the two tetracyclic endiandric acid skeletons, one with a ∆ 4,5 and ∆ 8,9 and the other with a ∆ 5,6 and ∆ 8,9 [4]. Key HMBC correlations (Figures 2 and S25) observed between the oxymethylene proton at δH 4.12 (2) and the carbons at δC 34.4 (C-7, 2), 126.0 (C-5, 2), and 141.6 (C-6, 2) and also between the olefinic proton at δH 5.74 (H-5, 2) and the carbons at δC 34.4 (C-7, 2), 36.8 (C-3, 2), and 65.5 (C-1′′, 2) were useful to locate the methylene group at C-4 in compound 2. Based on these data, it was concluded that compounds 1 and 2 were two new ∆ 4,5 and ∆ 8,9 and ∆ 5,6 and ∆ 8,9 alcohols with endiandric acid skeletons, to which the trivial names beilschmiedol B and C ( Figure 1) were given, respectively. The stereochemistry of all the endiandric acid derivatives was deduced from their biosynthesis. They are products of electrocyclic ring closures of naturally occurring polyketides, resulting from both the shikimate and acetate pathways. Their biosynthesis from the polyketide contains two consecutive non-enzymatic electrocyclic reactions, followed by an intramolecular Diels-Alder reaction [4,20,21,25]. As a result of the whole reaction sequence, an open-chain compound is converted into a tetracyclic compound. The starting product contains a conjugated tetraene system, as well as a conjugated diene system. Thus, it already displays the π electron systems required for the three pericyclic reactions; they are the two electrocyclizations and the Diels-Alder reaction [4,20,21,25]. The stereochemistry of all the endiandric acid derivatives was deduced from their biosynthesis. They are products of electrocyclic ring closures of naturally occurring polyketides, resulting from both the shikimate and acetate pathways. Their biosynthesis from the polyketide contains two consecutive non-enzymatic electrocyclic reactions, followed by an intramolecular Diels-Alder reaction [4,20,21,25]. As a result of the whole reaction sequence, an open-chain compound is converted into a tetracyclic compound. The starting product contains a conjugated tetraene system, as well as a conjugated diene system. Thus, it already displays the π electron systems required for the three pericyclic reactions; they are the two electrocyclizations and the Diels-Alder reaction [4,20,21,25].
Compound 3 was isolated as colorless oil. The molecular formula C 16 H 24 accounting for five double bond equivalents was assigned based on NMR data ( Figures S26-S30) and HRESIMS, which showed the sodium adduct peak [M + Na] + at m/z 239.1540 (calcd 239.1770 for C 16 H 24 Na). The 13 C-NMR spectrum of compound 3 ( Table 3) exhibited signals of 16 carbons, which were sorted by DEPT and HSQC into one quaternary carbon at δ C 142.9 (C-1 ), seven olefinic methine groups at δ C 125.6 (C-4 ), 128.4 (C-2 /6 ), 128.2 (C-3 /5 ), 130.1 (C-5), and 130.2 (C-6), seven methylene groups between δ C 22.6 and 36.0, and one methyl group at δ C 14.1 (C-10). Its 1 H-NMR spectrum displayed signals for a triplet of one methyl at δ H 0.80 (3H, t, J = 6.4 Hz, H-10), methylene groups between δ H 1.19 and 2.70, two olefinic protons at δ H 5.28 (2H, t, H-5,6), and a monosubstituted aromatic ring between δ H 7.10 and 7.20. From this information, it can be deduced that compound 3 is an n-alkene with a phenyl moiety. The C-5/C-6 position of the double bond was deduced from the HRESIMS, which showed an intense peak at m/z 173.0524 corresponding to the loss of a C 3 H 7 -fragment. The geometry of the double bond was assigned as cis based on the chemical shift of C-4 (27.3) and C-7 (25.7). It is known that the signals of the carbons next to a trans double bond appear at δ ≈ 32, while those of a cis double bond appear at δ ≈ 27 [26,27]. The identification of the carbon atoms next to the double was made through the HMBC correlations of H-5/C-4, H-6/C-7, H 2 -4/C-5, and H 2 -7/C-6. However, most of these data were close to those of the previously described synthetic compound, benzene-5E-decen-1-yl with clear difference at the level of carbons adjacent to the double bond, which confers the Z configuration to compound 3 [28]. Based on the above data, compound 3 was assigned as a new stereoisomer, (5Z) 1-phenyldec-5-ene, to which the trivial name obscurene (Figure 1) was given.  Figure S31) of 4 displayed characteristic signals for an aliphatic compound (including a methyl group at δ C 14.1 (C-25) and a long chain carbon signals between 29.4 and 29.7) with terminal double bond (including a methylene at δ C 139.3 (C-1) and an olefinic methine at δ C 114.1 (C-2)). The 1 HNMR spectrum ( Figure S32 and from the cork of the wine through gas chromatography-mass spectrometry (GCMS) [9,10] is isolated here for the first time and its NMR data reported.

In Vitro Antiplasmodial and Antitrypanosomal Activities
Extracts and isolated compounds from both plants were screened for their in vitro antiparasitic activity against the Plasmodium falciparum strain Pf3D7 and Trypanosoma brucei brucei ( Table 5). Samples that reduced parasite viability to < 20% were considered for dose-response assay to determine the IC 50 value. The MeOH/CH 2 Cl 2 (1:1) extract from the roots of B. louisii exhibited very good antitrypanosomal activity against Tb brucei with an IC 50 value of 4.6 µg/mL, while the extracts of stem bark of B. obscura and leaves of B. louisii exhibited moderate activities against the same strain with IC 50 values of 21.6 and 13.8 µg/mL, respectively (Table 5). The non-alkaloid fraction of the roots extract of B. louissi was moderately active, while the alkaloid rich fraction was non-active. Regarding the leaves, only the total extract showed a moderate activity. In both cases, the synergetic effect of the alkaloid and the non-alkaloid fractions could be evident.
Each extract was partitioned into alkaloid and neutral fractions. Alkaloid fractions and neutral fractions were also evaluated for their antitrypanosomal and antimalarial activities. The alkaloid fractions of the stem bark of B. obscura exhibited moderate antitrypanosomal activity with an IC 50 value of 16.9 µg/mL. Amongst the neutral fractions, only that of the roots of B. louisii showed moderate antitrypanosomal activity against Tb brucei in vitro with an IC 50 value of 20.9 µg/mL.
The isolated compounds and synthetic derivatives were also tested for their antitrypanosomal and antimalarial activities. Compounds 1-2 are position isomers (double bond) while 6-7 are chain (length) isomers. Both mixtures were showing a single spot on TLC card, indicating their same retardation factor. All the attempts to separate such isomers were unsuccessful independently to the different separation techniques used. Among the tested compounds, the mixtures 1-2, 6-7, and 13 showed good antitrypanosomal activity with IC 50 values of 4.91, ≤15.2, and 9.50 µM, respectively. From the synthetic derivatives, compound 11b showed the best antitrypanosomal activity with an IC 50 value of 8.3 µg/mL while the starting compound, beilschmiedic acid E was inactive against the same strain. Its alcohol derivative (11a) exhibited moderate activity. From the results obtained, it appears that the most active compounds are alcohols. The presence of this functional group may be important for the activity. In addition, the activity of the mixtures 1-2 and 6-7 may be due to synergetic action of their constituents. The antitrypanosomal potential of endiandric acid derivatives is reported here for the first time. The active mixture 6-7 consists of two lactones, class of secondary metabolites known whose antitrypanosomal potential was reported previously by Oleivera et al. (2019) [29].
Cytotoxicity assays were also carried out on the extract and compounds, and they were found to be non-toxic. With regard to the antiplasmodial activity, only the synthetic derivative 11b exhibited a moderate activity with an IC 50 value of 31.42 µM.
To the best of our knowledge, B. louisii has not yet been subject of chemical and biological investigation. The Lauraceae family remains the only source of bioactive endiandric acid derivatives. Beilschmiedia and Endiandra genus (Lauraceae) are well known for a long time as a rich source of biologically active secondary metabolites. They are still the only source of this class of secondary metabolites [30]. The genus Beilschmiedia is a pantropical genus of about 287 species, only 24 species have been studied phytochemically so far. Endiandric acid derivatives are major constituents of these genera, and are important chemotaxonomic markers used to identify Beilschmiedia plant species from a phytochemical point of view [3]. In previous studies, endiandric acid has been isolated from B. obstusifolia, B. introrsa, B. jonesii, B. toorom, B. oligandra, B. manii, B. fulva, B. anarcardioides, B. erythrophoia, B. tsangii, B. cryptocaryoides, and B. ferruginea. This is the first time that this class of secondary metabolites is reported from B. louisii and B. obscura. This study is important as far as the chemotaxonomy of Beilschmiedia species is concerned and, and highlights the antitrypanosomal potential of endiandric acid and lactone derivatives.

Chemicals and Reagents
The organic solvents (from Fisher, Waltham, MA, US) used for the extraction of plant material and for column chromatography (CC) were of technical and laboratory grade, while those use in reactions were of analytical grade. For column chromatography, the main solvents used were n-hexane, dichloromethane, ethyl acetate, and methanol, used as pure or binary mixtures at different concentrations for purification of compounds. Silica gel 230-400 and 70-230 mesh (Merck) were used for column chromatography with different mixtures of n-hexane-ethyl acetate, and dichloromethane-methanol solvent systems as eluents. Iodine or vanillin reagent (1.0 g of vanillin in 70 mL ethanol 96% + 10 mL concentrated sulfuric acid) was used to reveal the spots of compounds on TLC cards.

Instruments and Apparatus
NMR spectra were recorded on Bruker DRX-600 MHz spectrometer (Bruker, Rheinstetten, Germany) operating at 75, 100, 150, 300, 400, and 600 MHz, where chemical shifts (δ) are given in ppm with reference to the TMS signal. HR-ESI-MS were recorded on a Bruker Compact Q-TOF mass spectrometer (Bremen, Germany) equipped with DionexUltiMate 3000 UHPLC and electrospray ionization (ESI). For the direct infusion MS, the spectrometer operated in positive and negative modes (mass range: 100-1500, with a scan rate of 1.00 Hz) with automatic gain control to provide high-accuracy mass measurements within 1 ppm deviation using sodium formate as calibrant. The spray voltage was 4.5 kV with a capillary temperature of 200 • C. The flow rate of sample was 180 µL/h and nitrogen were used as sheath gas (4 L/min). Pre-coated aluminum silica gel 60 F 254 sheets were used for TLC. Spots were visualized with UV light (254 and 365 nm).

Plant Material
The

Extraction and Isolation
The air-dried roots (2.5 kg) and leaves (1.0 kg) of B. louisii, and stem bark (2.0 kg) of B. obscura, were separately ground and extracted with a mixture of CH 2 Cl 2 -MeOH (1:1) for 48 h at room temperature each (about 25 • C). The different filtrates were freed from solvent under vacuum to give the crude extracts (roots (350.0 g, 14.0%) and leaves (130.0 g, 13.0%) of B. louisii, and stem bark (300.0 g, 7.0%) of B. obscura). Each extract was partitioned into neutral (roots (300.0 g) and leaves (80.0 g) of B. louisii, and stem bark (200.0 g) of B. obscura) and alkaloid (roots (8.6 g) and leaves (2.0 g) of B. louisii, and stem bark (2.0 g) of B. obscura) rich fractions, as described by Pudjastuti [31]. Each crude extract was separately dissolved in cold distilled water and defatted with n-hexane. The aqueous layers were then acidified with hydrochloric acid (5% HCl) to pH 3 to obtain acidic solutions that were then extracted with dichloromethane to lead to neutral fractions. The remaining aqueous acidic phases were then made alkaline by adding ammonium hydroxide (25% NH 4 OH) solution to pH 11 and extracted with dichloromethane to afford the alkaloid fractions.
The neutral fraction (280 g) of roots B. louisii was also subjected to flash chromatography as the previous one to yield sub-fractions (F-L). Fraction G (40 g) was subjected to repeated column chromatography over silica gel (70-230 mesh, Merck, 300 g) and eluted with n-hexane and resulted in the isolation of the binary mixture of beilschmiedic acid A and beilschmiedic acid C (8 and 9, 80 mg). Fraction F (100 g) was also subjected to column chromatography over silica gel (70-230 mesh, Merck, 200 g) and eluted with a mixture of n-hexane/EtOAc (9:1→7:3) to yield compounds beilschmiedic acid D (10, 11 mg) and beilschmiedic acid E (11, 10 g). Fraction H (17 g) was treated as fraction G to yield methylenedioxyendiandric acid A (12, 60 mg) and β-sitosterol (13, 50 mg). Fraction K (10 g) was purified on a silica gel (70-230 mesh, Merck, 50 g) column with the isocratic mixture of n-hexane/EtOAc (1:1) as eluent to yield compounds epicatechin (14, 10 mg) and β-sitosterol-3-O-β-D-glucopyranoside (15, 75 mg). The neutral fraction of the leaves afforded β-sitosterol (13, 25 mg), hydrocarbons, and fatty acid compounds, which structures were not determined. Repeated column chromatography of alkaloid fractions of the roots of B. louisii and the stem bark of B. obscura over alumina gel afforded five compounds that were not characterized due to the non-solubility in NMR solvents.

Synthesis of Compound 11a (Beilschmiedol A)
The solution of Beilschmiedic acid E (11) 3 mmol in THF (10 mL) was cooled to −5 • C using ice/NaCl bath and lithium aluminum hydride (LiAlH 4 ), 3.5 mmol, was carefully added to the above solution in portions, while maintaining the temperature lower than 0 • C. The mixture was then allowed to rise to room temperature gradually and stirred at room temperature for 48 h. The mixture was quenched using petroleum ether (50 mL) and a solution of 10% of NaOH (30 mL) was added dropwise. The mixture was then extracted with petroleum ether (3 × 50 mL). The organic phases were combined, washed with the saturated brine and dried over MgSO4. The solvent was removed from the resulting solution under vacuum to afford compound 11a as an off-yellow oil (980 mg, 98%).

Synthesis of Compound 11b (Beilschmiedal)
In a 100 mL round bottom flask equipped with a magnetic stirrer, a solution of Dess-Martin periodinane (DMP) reagent (3.3 mmol), dissolved in methylene dichloride (DCM, 10 mL) was placed. In a beaker, compound 11a (3 mmol) was dissolved in 10 mL of DCM, and then slowly added to the DMP solution while stirring. The mixture was stirred at room temperature for 24 h. The reaction mixture was diluted with diethyl ether (50 mL), then quenched by pouring it into a 250 mL beaker containing saturated aqueous sodium bicarbonate solution (15 mL) with sodium thiosulfate (2.5 g). The mixture was stirred for 15 min and transferred into a separation funnel where the layers were allowed to separate. The aqueous layer was discarded, and the organic layer washed with water and saturated brine, successively, and dried over MgSO4. The organic layer was then freed from solvent under vacuum and purified on column chromatography (silica gel; hex-AE (9:1)) to yield compound 11b as an off-yellow oil (350 mg, 70%).

Synthesis of Compound 11c (Beilschmiecarbazone)
In a round bottom flask, compound 11b (20 mmol) was dissolved in 100 mL of ethanol. A solution of thiosemicarbazide reagent (20 mmol dissolved in 20 mL of 1 N hydrochloric acid) was prepared in a beaker and added into a round bottom flask containing the compound 11b. The mixture was stirred at room temperature (at about 25 • C) to afford compound 11c (245 mg, 70%) as a precipitate. The precipitate was filtered, dried, and then recrystallized in ethanol (96 • C). was allowed to stir at room temperature overnight, and urea was filtered off using a kieselguhr plug. The organic solvent was removed under reduced pressure and the organic residue was purified on column chromatography over silica gel using CH 2 Cl 2 as a solvent to yield compound 11d (400 mg, 80%).

In Vitro Antiplasmodial Assay
The antiplasmodial assay was carried out using the pLDH assay as described by Makler. [32] with little modification against the chloroquine-sensitive 3D7 strain of P. falciparum (Pf 3D7). Malaria parasites (Pf 3D7) were maintained in RPMI 1640 medium containing 2 mM L-glutamine and 25 mM Hepes (Lonza) further supplemented with 5% Albumax II, 20 mM glucose, 0.65 mM hypoxanthine, 60 µg/mL gentamycin, and 2-4% hematocrit human red blood cells. The parasites were cultured at 37 • C under an atmosphere of 5% CO 2 , 5% O 2 , and 90% N 2 in a sealed T75 culture flask. Single concentration screening was conducted using solutions of 50 µg/mL of the extract and 20 µg/mL of pure or combined compounds. The combinations were obtained by mixing solutions of single corresponding compounds volume by volume. Samples were further added to parasite cultures in 96-well clear plates and incubated in a 37 • C CO 2 incubator. After 48 h, 20 µL of culture was removed from each well and combined with 125 µL of a mixture of Malstat and NBT/PES solutions in a fresh 96-well plate. These solutions measured the activity of the parasite lactate dehydrogenase (pLDH) enzyme in the cultures. A purple product formed when pLDH was active, was then quantified by Spectramax M3 microplate reader (Abs 620 ) from Molecular Devices LLC. The Abs 620 reading in each well reflected the pLDH activity and hence the number of parasites present. For each sample concentration, % parasite viability-the pLDH activity in sample treated wells relative to untreated controls was calculated. Samples were tested in triplicate and standard deviations (SD) derived. Samples that reduced parasite viability to ≤20% from the single concentration assay were used in a dose response assay to determine the 50% inhibitory concentration (IC 50 ) values. For that, samples were serially diluted and tested as described by Makler et al. [32]. Samples were tested in triplicate and for each, the percentage viability was plotted against Log (concentration) and the IC 50 value obtained from the resulting dose-response curve by non-linear regression. Chloroquine was used as a drug standard and yielded IC 50 values in the range 0.008 µg/mL.

In Vitro Antitrypanosomal Assay
To assess the trypanocidal activity, samples were added to cultures of Tb brucei in 96-well plates at a fixed concentration of 20 µg/mL for single and combined compounds and 50 µg/mL for the extracts. After 48 h of incubation, parasites surviving drug treatment were measured by adding a resazurin. Resazurin is reduced to resorufin (a fluorophore (Exc 560 /Em 590 )) in viable cells and was quantified using a Spectramax M3 microplate reader. Results were expressed as % parasite viability-the resorufin fluorescence in sample treated wells relative to untreated controls. Samples were tested in triplicate and standard deviations (SD) derived. Samples that reduced parasite viability to < 20% were considered for dose-response assay to determine the IC 50 value. In brief, samples were serially diluted and tested in duplicate. Percentage viability was plotted against Log (concentration) and the IC 50 obtained from the resulting dose-response curve by non-linear regression. Pentamidine was used as a drug standard and yielded IC 50 values in the range 0.01 µg/mL [33].

Cytotoxicity Assay
Human cervix adenocarcinoma cells (HeLa cells) were used to check the toxicity of the crude extract and compounds and their combinations as described by Keusch et al. (1972) [34]. Stocks solutions were prepared in DMSO at a concentration of 50 µg/mL for the extracts and 20 µM for compounds, and combinations. To assess the overt cytotoxicity, samples were placed in 96-well plates containing cells for 48 h. The numbers of cells surviving drug treatment were determined using the resazurin based reagent and reading resorufin fluorescence in a Spectramax M3 microplate reader. Results were expressed as % viability-the resorufin fluorescence in compound-treated wells relative to untreated controls. Compounds were tested in duplicate and standard deviations (SD) derived. Emetine (which induces cell apoptosis) was used as the positive control.

Conclusions
Phytochemical investigation of the MeOH/CH 2 Cl 2 (1:1) extract of the roots and leaves of Beilschmiedia louisii and B. obscura, which showed potent antitrypanosomal activity during preliminary screening on T. brucei led to the isolation of a mixture of two new endiandric acid derivatives 1 and 2, and one new phenylalkene (3) along with twelve known compounds (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). In addition, four new derivatives (11a, 11b, 11c, and 11d) were synthesized from compound 11. Furthermore, the isolates were evaluated for their antitrypanosomal and antimalarial activities against Tb brucei and the P. falciparum chloroquine-resistant strain Pf3D7 in vitro, respectively. From the tested compounds, the mixture of the new compounds exhibited potent antitrypanosomal activity in vitro with IC 50 value of 1.61 µg/mL. The extracts and compounds from both plants were not toxic against human cervix adenocarcinoma cells (HeLa cells) and the extract of the roots of B. louisii exhibited the good antitrypanosomal activity with an IC 50 value of 4.62 µg/mL.