The Geraniin-Rich Extract from Reunion Island Endemic Medicinal Plant Phyllanthus phillyreifolius Inhibits Zika and Dengue Virus Infection at Non-Toxic Effect Doses in Zebrafish

The mosquito-borne viruses dengue (DENV) and Zika (ZIKV) viruses are two medically important pathogens in tropical and subtropical regions of the world. There is an urgent need of therapeutics against DENV and ZIKV, and medicinal plants are considered as a promising source of antiviral bioactive metabolites. In the present study, we evaluated the ability of Phyllanthus phillyreifolius, an endemic medicinal plant from Reunion Island, to prevent DENV and ZIKV infection in human cells. At non-cytotoxic concentration in vitro, incubation of infected A549 cells with a P. phillyreifolius extract or its major active phytochemical geraniin resulted in a dramatic reduction of virus progeny production for ZIKV as well as four serotypes of DENV. Virological assays showed that P. phillyreifolius extract-mediated virus inhibition relates to a blockade in internalization of virus particles into the host cell. Infectivity studies on ZIKV showed that both P. phillyreifolius and geraniin cause a loss of infectivity of the viral particles. Using a zebrafish model, we demonstrated that administration of P. phillyreifolius and geraniin has no effect on zebrafish locomotor activity while no morbidity nor mortality was observed up to 5 days post-inoculation. Thus, P. phillyreifolius could act as an important source of plant metabolite geraniin which is a promising antiviral compound in the fight against DENV and ZIKV.


Introduction
Mosquito-borne dengue (DENV) and Zika (ZIKV) viruses belonging to the flavivirus genus (Flaviviridae family) are two major concerns in public health worldwide [1]. The geographic range of DENV spreads from tropical to subtropical regions [2,3]. Most hyperendemic regions are located in Southeast Asia and Latin America where severe outbreaks of dengue disease occur annually. The four (Table 1). A concentration of 250 µg/mL of P. phillyreifolius extracts that maintained 95%-100% of cell viability ( Figure 1A) was consequently chosen for testing potential anti-ZIKV activity. A chimeric molecular clone of African strain of ZIKV expressing a GFP reporter gene (ZIKV GFP ) was used for monitoring viral infection by flow cytometer in A549 cells where ZIKV can replicate efficiently [39]. Thus, A549 cells were infected 24 h with ZIKV GFP in the presence of different non-toxic concentrations of P. phillyreifolius. A dose-dependent effect of P. phillyreifolius on ZIKV infectivity was observed with a complete inhibition of ZIKV infection at non-cytotoxic concentrations of plant extract ( Figure 1B). The concentration that inhibits 50% of ZIKV infection (IC 50 ) was calculated as 55 µg/mL for P. phillyreifolius (Table 1).
Molecules 2020, 25, x FOR PEER REVIEW 3 of 17 molecular clone of African strain of ZIKV expressing a GFP reporter gene (ZIKV GFP ) was used for monitoring viral infection by flow cytometer in A549 cells where ZIKV can replicate efficiently [39]. Thus, A549 cells were infected 24 h with ZIKV GFP in the presence of different non-toxic concentrations of P. phillyreifolius. A dose-dependent effect of P. phillyreifolius on ZIKV infectivity was observed with a complete inhibition of ZIKV infection at non-cytotoxic concentrations of plant extract ( Figure 1B). The concentration that inhibits 50% of ZIKV infection (IC50) was calculated as 55 µg/mL for P. phillyreifolius (Table 1). To further validate the anti-ZIKV effect of P. phillyreifolius, A549 cells were infected with the epidemic Asian strain ZIKV-PF13, which was responsible for the 2015 epidemic in French Polynesia [40], during 24 h in the presence of increasing concentrations of P. phillyreifolius. At non-cytotoxic  To further validate the anti-ZIKV effect of P. phillyreifolius, A549 cells were infected with the epidemic Asian strain ZIKV-PF13, which was responsible for the 2015 epidemic in French Polynesia [40], during 24 h in the presence of increasing concentrations of P. phillyreifolius. At non-cytotoxic concentrations, the amount of intracellular viral RNA determined by RT-qPCR was reduced up to 3.5 log ( Figure 1C). Quantification of viral growth by plaque-forming assay revealed that ZIKV-PF13 was sensitive to different P. phillyreifolius concentrations ( Figure 1D). Indeed, as observed for the African strain ZIKV GFP , P. phillyreifolius lowered ZIKV-PF13 progeny production up to 3-log at non-cytotoxic doses with a dose-dependent manner ( Figure 1D). In parallel, viral protein production was evaluated 24 h post infection (hpi) by immunofluorescence assay using an anti-flavivirus E mAb 4G2 ( Figure 1E). Our data showed that the production of the viral protein E in A549 cells was severely affected after P. phillyreifolius treatment even at lower concentrations ( Figure 1E). Together, these results showed that P. phillyreifolius exerts a potent antiviral effect against an African and an Asian strain of ZIKV.
We asked whether P. phillyreifolius extract also exerts antiviral activity against DENV, a closely related flavivirus to ZIKV. The antiviral activity of P. phillyreifolius was assessed on the four different serotypes (clinical isolates for DENV-1 to DENV-3 and a DENV-4 laboratory-adapted strain). The human hepatoma cell lines Huh7.5, which are permissive to DENV infection, were used to perform these experiments ( Figure 2). Production of infectious DENV particles from Huh7.5 cells in presence of P. phillyreifolius (250 µg/mL) was quantified using foci forming immunodetection assays ( Figure 2). A decrease of up to 3-log of DENV infectivity was observed upon DENV1-4 ( Figure 2). These results show that P. phillyreifolius extract exhibits potent antiviral activity against the four DENV serotypes.
Molecules 2020, 25, x FOR PEER REVIEW  4 of 17 concentrations, the amount of intracellular viral RNA determined by RT-qPCR was reduced up to 3.5 log ( Figure 1C). Quantification of viral growth by plaque-forming assay revealed that ZIKV-PF13 was sensitive to different P. phillyreifolius concentrations ( Figure 1D). Indeed, as observed for the African strain ZIKV GFP , P. phillyreifolius lowered ZIKV-PF13 progeny production up to 3-log at non-cytotoxic doses with a dose-dependent manner ( Figure 1D). In parallel, viral protein production was evaluated 24 h post infection (hpi) by immunofluorescence assay using an anti-flavivirus E mAb 4G2 ( Figure  1E). Our data showed that the production of the viral protein E in A549 cells was severely affected after P. phillyreifolius treatment even at lower concentrations ( Figure 1E). Together, these results showed that P. phillyreifolius exerts a potent antiviral effect against an African and an Asian strain of ZIKV.
We asked whether P. phillyreifolius extract also exerts antiviral activity against DENV, a closely related flavivirus to ZIKV. The antiviral activity of P. phillyreifolius was assessed on the four different serotypes (clinical isolates for DENV-1 to DENV-3 and a DENV-4 laboratory-adapted strain). The human hepatoma cell lines Huh7.5, which are permissive to DENV infection, were used to perform these experiments ( Figure 2). Production of infectious DENV particles from Huh7.5 cells in presence of P. phillyreifolius (250 µg/mL) was quantified using foci forming immunodetection assays ( Figure  2). A decrease of up to 3-log of DENV infectivity was observed upon DENV1-4 ( Figure 2). These results show that P. phillyreifolius extract exhibits potent antiviral activity against the four DENV serotypes.

Geraniin, the major compound of Phyllanthus phillyreifolius ethanolic extract, inhibits ZIKV infection at non-cytotoxic concentrations.
We recently characterized the phytochemical composition of P. phillyreifolius ethanolic extract using UPLC-QTOF-MS [32]. We have demonstrated that P. phillyreifolius extract contains six phenolic components in which geraniin is the major quantitatively constituent, followed by ellagic acid, elaeocarpusin, rutin, quercetin, and gallic acid [32,33]. It has been reported that geraniin is a potent inhibitor of DENV-2 [34]. Consequently, DENV served as a flavivirus positive control in the following experiments.
Thus, we decided to investigate the anti-ZIKV activity of geraniin. We first tested its cytotoxicity on A549 cells using an MTT assay ( Figure 3A). The CC50 was calculated as 420 µg/mL ( Table 1). The concentration of 200 µg/mL of geraniin, which maintained 95%-100% of A549 cell viability ( Figure  3A), was chosen to test the potential antiviral activity of geraniin on A549 cells. As previously Huh7.5 cells were infected for 48 h with DENV at different MOI 0.2, 2, 0.5, and 2 for DENV 1-4, respectively, with or without P. phillyreifolius (250 µg/mL). The virus progeny production was titrated in C6/36 cells using a foci-forming immunodetection assay. Data represent the means ± SD from three independent experiments performed in duplicate. ** p < 0.01; *** p < 0.001, n.s. = not significant.

Geraniin, the Major Compound of Phyllanthus phillyreifolius Ethanolic Extract, Inhibits ZIKV Infection at Non-Cytotoxic Concentrations
We recently characterized the phytochemical composition of P. phillyreifolius ethanolic extract using UPLC-QTOF-MS [32]. We have demonstrated that P. phillyreifolius extract contains six phenolic components in which geraniin is the major quantitatively constituent, followed by ellagic acid, elaeocarpusin, rutin, quercetin, and gallic acid [32,33]. It has been reported that geraniin is a potent inhibitor of DENV-2 [34]. Consequently, DENV served as a flavivirus positive control in the following experiments.
Thus, we decided to investigate the anti-ZIKV activity of geraniin. We first tested its cytotoxicity on A549 cells using an MTT assay ( Figure 3A). The CC 50 was calculated as 420 µg/mL ( Table 1). The concentration of 200 µg/mL of geraniin, which maintained 95%-100% of A549 cell viability ( Figure 3A), was chosen to test the potential antiviral activity of geraniin on A549 cells. As previously described for P. phillyreifolius, the antiviral activity of geraniin was assessed using ZIKV GFP . FACS assay showed that geraniin severely restricted ZIKV infection in A549 cells, yielding 90% inhibition of infection at 100 µg/mL ( Figure 3B). The IC 50 value was 22 µg/mL (Table 1). Based on the determined cytotoxicity and antiviral efficacy, the Selectivity Index (SI) was estimated, according to the values of CC 50 and IC 50 (SI = CC 50 /IC 50 ), as 13 and 19 for P. phillyreifolius and geraniin, respectively (Table 1). Cytotoxic concentration (CC 50 ) and inhibitory concentration (IC 50 ) were obtained by performing nonlinear regression followed by the construction of a sigmoidal concentration-response curves from Figure 1A,B. a Concentration that inhibited cell viability by 50%; b concentration that inhibited infection by 50%; c selectivity index (CC 50 /IC 50 ).
The antiviral activity of geraniin was next evaluated against the Asian epidemic ZIKV strain (ZIKV-PF13). For this purpose, A549 cells were inoculated with ZIKV-PF13 in presence of non-cytotoxic concentrations of geraniin. Dose-response RT-qPCR analysis showed that geraniin severely restricted ZIKV-PF13 replication in A549 cells yielding a 3-log reduction of viral RNA copies at 200 µg/mL ( Figure 3C). Likewise, ZIKV-PF13 progeny production was severely inhibited in a concentration-dependent manner using a plaque forming assay ( Figure 3D). Geraniin reduced the viral progeny production by at least 3.5-log at 200 µg/mL ( Figure 3D). In parallel, immunofluorescence analysis using anti-flavivirus E mAB 4G2 confirmed that geraniin severely restricted E-ZIKV production in A549 cells 24 hpi ( Figure 3E). DENV1-4 were used as a flavivirus positive control to assess the antiviral activity of geraniin ( Figure 3F). As expected from a previous study [34], geraniin severely restricted DENV-2 growth, as well as growth of the three other DENV serotypes, in Huh7.5 at non-cytotoxic concentrations. Indeed, as observed for ZIKV, geraniin lowered DENV progeny production of the four DENV serotypes up to 3-log at 200 µg/mL. Altogether, these data demonstrate that geraniin can efficiently inhibit ZIKV and DENV infection in human cells in a dose-dependent manner and reflect its potential as a source of natural antiviral phytochemical.  The results shown are means ± SD of four independent experiments and are expressed as relative value compared to untreated infected cells. ** p < 0.01; *** p < 0.001, n.s. = not significant (E) Immunofluorescence analysis of viral protein expression in ZIKV-PF13-infected A549 cells. The ZIKV (red) and nuclei (blue) were visualized by fluorescence microscopy. Scale bars are 50 µm. Results from a representative experiment (n = 3 repeats) are shown. (F) DENV was used as a flavivirus positive control. Huh7.5 cells were infected for 48 h with DENV at different MOI 0.2, 2, 0.5 and 2 for DENV 1-4 respectively with or without geraniin (200 µg/mL). The virus progeny production was titrated in C6/36 cells using a foci-forming immunodetection assay. Data represent the means ± SD from three independent experiments performed in duplicate.

Phyllanthus phillyreifolius Ethanolic Extract and Geraniin Prevent ZIKV Entry in A549 Cells
Time-of-drug addition approach [41] was performed to determine which stages of ZIKV infection are targeted by P. phillyreifolius and whether geraniin is the main active antiviral component present in plant extract ( Figure 4A). When A549 cells were treated with a non-cytotoxic concentration of geraniin (200 µg/mL) or P. phillyreifolius (250 µg/mL) throughout the experiment or concomitantly with virus input for 2 h (entry), the fluorescence intensity representing viral replication was detected in less than 5% of cells ( Figure 4B, "Throughout", "Entry"). However, no significant effect was observed when P. phillyreifolius or geraniin were added 2 h post adsorption ( Figure 4B, "Replication"). These results suggest that the antiviral activity of P. phillyreifolius and geraniin was not mediated by the inhibition of the replication stage but rather by the inhibition of early steps of viral infectious cycle. To investigate whether P. phillyreifolius extract or geraniin render virus particles unable to initiate viral infection in the host cell ( Figure 4A, "Free virus"), ZIKV GFP particles were incubated 2 h at 37 • C with P. phillyreifolius (250 µg/mL) or geraniin (200 µg/mL) then diluted 50-fold prior to A549 cell infection. This dilution titrates the plant extract or geraniin below their therapeutic concentrations and prevents potential interactions with the host cell surface. Our results showed that such treatment of ZIKV GFP resulted in 99% reduction of GFP-positive cells at 24 hpi compared to cells infected with untreated ZIKV GFP ( Figure 4B, Free virus). These results suggest that phytochemicals present in P. phillyreifolius extract, mainly geraniin, interact with ZIKV-free particles, irreversibly, to prevent infection. This observation suggests that geraniin could bind to ZIKV particles resulting in disassembly of ZIKV particles or affecting their infectivity. To investigate this hypothesis, an RNase protection assay [25] was performed to determine whether P. phillyreifolius extract causes virus particle disassembly leading to a release of genomic RNA ( Figure S1). Given that viral genomic RNA displayed a complete resistance to RNase A ( Figure S1), we can exclude the hypothesis that P. phillyreifolius-mediated inhibition of ZIKV relates to a loss of virion integrity. Taken together, these results suggest that P. phillyreifolius extract acts in preventing the initiation of the virus infectious cycle, presumably through a blockade of the virus entry steps into the host cell.
To determine whether geraniin and/or other phytochemicals present in P. phillyreifolius extract preclude the attachment of ZIKV particles to cell membrane, prechilled ZIKV GFP and P. phillyreifolius or geraniin were mixed and allowed to bind A549 cells at 4 • C for 1 h to allow virus binding and prevent its entry ( Figure 4C). Epigalocathechin gallate (EGCG), which is known to inhibit ZIKV attachment was used as a positive control [29,41]. After 1 h of ZIKV attachment in presence or absence of P. phillyreifolius or geraniin, cells were rinsed and the number of attached ZIKV particles was evaluated using RT-qPCR ( Figure 4C). Our data showed that the number of viral particles attached to the cell surface in presence of P. phillyreifolius or geraniin was similar to untreated cells ( Figure 4C). As expected from previous studies, the amount of ZIKV particles attached to cell membrane was severely reduced in the presence of EGCG [29,41]. These results suggest that neither geraniin nor any other phytochemical present in plant extract were capable of interfering with the ZIKV attachment step. To determine whether geraniin and/or other phytochemicals present in P. phillyreifolius extract preclude the attachment of ZIKV particles to cell membrane, prechilled ZIKV GFP and P. phillyreifolius or geraniin were mixed and allowed to bind A549 cells at 4°C for 1 h to allow virus binding and prevent its entry (Figure 4 C). Epigalocathechin gallate (EGCG), which is known to inhibit ZIKV attachment was used as a positive control [29,41]. After 1 h of ZIKV attachment in presence or absence of P. phillyreifolius or geraniin, cells were rinsed and the number of attached ZIKV particles was evaluated using RT-qPCR ( Figure 4C). Our data showed that the number of viral particles attached to the cell surface in presence of P. phillyreifolius or geraniin was similar to untreated cells ( Figure 4C). As expected from previous studies, the amount of ZIKV particles attached to cell membrane was severely reduced in the presence of EGCG [29,41]. These results suggest that neither geraniin nor any other phytochemical present in plant extract were capable of interfering with the ZIKV attachment step.
The effect of P. phillyreifolius extract and its main compound on a subsequent post-adsorption step of the virus infectious cycle was next analyzed, thereby testing their effect on the kinetics of ZIKV internalization ( Figure 4D). Thus, ZIKV GFP was allowed to bind to the cell surface at 4°C followed by a shift of temperature to 37 °C. At different time points after temperature shift, cells were washed and then incubated with P. phillyreifolius extract or geraniin ( Figure 4D). ZIKV-infected A549 cells The effect of P. phillyreifolius extract and its main compound on a subsequent post-adsorption step of the virus infectious cycle was next analyzed, thereby testing their effect on the kinetics of ZIKV internalization ( Figure 4D). Thus, ZIKV GFP was allowed to bind to the cell surface at 4 • C followed by a shift of temperature to 37 • C. At different time points after temperature shift, cells were washed and then incubated with P. phillyreifolius extract or geraniin ( Figure 4D). ZIKV-infected A549 cells were examined for GFP-expression at 24 hpi. The natural flavonoid isoquercitrin (Q3G), which is known to inhibit ZIKV internalization, was used as positive control [25]. Flow cytometric analysis showed that P. phillyreifolius extract and geraniin are capable of inhibiting internalization step of ZIKV in a similar way to that of Q3G ( Figure 4D). Until 15 min after temperature shift, geraniin treatment resulted in a complete inhibition of viral entry with a reduction of GFP-positive cells by 90% ( Figure 4D). Neither P. phillyreifolius extract nor geraniin are able to inhibit viral infection when added 2 h post temperature shift. Moreover, both P. phillyreifolius extract and geraniin have an identical internalization inhibition profile, thus suggesting that geraniin is probably the main active compound present in the extract of P. phillyreifolius. These kinetics are also similar to those of the positive control Q3G ( Figure 4D). These results suggest that geraniin-mediated inhibition of ZIKV infection occurs early after virus binding to the cell membrane and could be explained by the incapacity of the ZIKV-attached particles to be internalized into the host cell in presence of geraniin.

Phyllanthus phillyreifolius and Geraniin Do Not Exhibit Acute Toxicity in Zebrafish
Zebrafish is a model organism widely used for studying in vivo toxicity during development and adulthood. It represents an interesting alternative to toxicity studies in rodents. In addition to sharing a high genomic homology with humans (>70%) [42], zebrafish also display many physiological processes common with mammals [43] that are interesting for toxicity studies, such as liver and renal metabolic functions. Consequently, the potential toxicity of P. phillyreifolius and geraniin was assessed in adult zebrafish as recently done for other medicinal plant extract such as Ayapana triplinervis essential oil [44]. To this aim, intraperitoneal injection was performed with the corresponding maximum non-toxic concentration (MNTC) of 375 microg/g and 300 microg/g of body weight for P. phillyreifolius and geraniin, respectively ( Table 2). Any striking signs of stress, suffering, feeding behavior, and abnormal behavior were carefully checked several times a day for a period of 5 days. As shown in Table 2, the injection of P. phillyreifolius and geraniin did not impact fish behavior (feeding and locomotion), and no sign of stress was reported (Table 2). Moreover, the P. phillyreifolius and geraniin treatments resulted in 100% fish survival, similar to the control injection with PBS ( Table 2). Taken together, these data suggest that these doses do not exhibit acute toxic effects. Table 2. Survival and behavior of fish injected with Phyllanthus phillyreifolius extract and geraniin from 1 day post injection to 5 days post injection (dpi).

Geraniin Does Not Disturb Zebrafish Locomotor Activity
In order to have a better idea of the potential effects of geraniin on the fine-tuned locomotor behavior, as a sign of stress, the locomotor activity of zebrafish was carefully monitored by performing a ZebraCube analysis ( Figure 5). After a 10 min period of adaptation into their new tanks, the locomotor activity of individual vehicle and geraniin-injected fish was monitored for a 10 min period at 1 and 5 days post injection (dpi). The results demonstrate that geraniin-injected fish traveled the same distance in an "inactive" state and in small and large activity states as their respective controls at 1 dpi and 5 dpi ( Figure 5A). In addition, the path traveled by vehicle and geraniin-injected fish are largely similar establishing that the geraniin injection has no obvious effect on the fine-tuned locomotor activity of fish ( Figure 5B). Consequently, it appears that the MNTC injection of geraniin did not induce adverse effects in zebrafish.

Discussion
In the absence of effective antivirals against ZIKV and DENV, these arthropod-borne flaviviruses have emerged as a serious health concern that affected millions of people in the last years [2,45]. Albeit a huge amount of research having been conducted in the last five years, there is still a lack of effective anti-ZIKV compounds. Within a project aiming to investigate the antiviral activity of Reunion Island (RUN) plant biodiversity, we recently identified several endemic or indigenous polyphenol-rich medicinal plants from RUN that inhibit ZIKV infection by preventing viral entry [41,46,47]. As we have recently demonstrated the strong antioxidant activity of P. phillyreifolius due to its high polyphenol content, mainly ellagitannin such as geraniin [32,33], we decided to investigate the anti-ZIKV activity of P. phillyreifolius and its major compound geraniin. We demonstrated here, using RT-qPCR, immunofluorescence, and plaque forming assays, that both African and Asian epidemic ZIKV strains [6] are sensitive to cell treatment with a non-cytotoxic concentrations of P. phillyreifolius and its major compound geraniin. We also demonstrated that P. phillyreifolius extract prevents infection of human cells by four DENV serotypes. We showed that P. phillyreifolius extract acts, probably through its major compound geraniin, on early steps of the viral replication cycle, in a similar way than isoquercitrin (Q3G), a natural flavonoid, which is known to inhibit ZIKV internalization into the host cell [25]. Indeed, RNase protection, inactivation, binding, and internalization assays suggest that P. phillyreifolius-mediated inhibition of ZIKV was not associated with a loss of viral integrity but rather with a neutralization of ZIKV infectivity allowing attachment and preventing internalization of ZIKV particles in the host cell. Geraniin-mediated inhibition of ZIKV is different from that described previously where geraniin extracted from Nephelium lappaceum inhibits DENV-2 binding to the host cell [34].
Phyllanthus is one of the largest genera in the family Phyllanthaceae that comprises over 700 species distributed mainly in the tropics and subtropics and is in constant use in traditional medicine to treat diverse human diseases [48]. Phyllanthus species represent an extensive phytochemical diversity, e.g., tannins, terpenes, alkaloids, flavones, saponins, etc. These chemical compounds derived from Phyllanthus species exhibit antiviral effects against a broad spectrum of enveloped RNA and DNA viruses [49][50][51][52][53][54][55]. The results presented in this study underscore endemic medicinal plants

Discussion
In the absence of effective antivirals against ZIKV and DENV, these arthropod-borne flaviviruses have emerged as a serious health concern that affected millions of people in the last years [2,45]. Albeit a huge amount of research having been conducted in the last five years, there is still a lack of effective anti-ZIKV compounds. Within a project aiming to investigate the antiviral activity of Reunion Island (RUN) plant biodiversity, we recently identified several endemic or indigenous polyphenol-rich medicinal plants from RUN that inhibit ZIKV infection by preventing viral entry [41,46,47]. As we have recently demonstrated the strong antioxidant activity of P. phillyreifolius due to its high polyphenol content, mainly ellagitannin such as geraniin [32,33], we decided to investigate the anti-ZIKV activity of P. phillyreifolius and its major compound geraniin. We demonstrated here, using RT-qPCR, immunofluorescence, and plaque forming assays, that both African and Asian epidemic ZIKV strains [6] are sensitive to cell treatment with a non-cytotoxic concentrations of P. phillyreifolius and its major compound geraniin. We also demonstrated that P. phillyreifolius extract prevents infection of human cells by four DENV serotypes. We showed that P. phillyreifolius extract acts, probably through its major compound geraniin, on early steps of the viral replication cycle, in a similar way than isoquercitrin (Q3G), a natural flavonoid, which is known to inhibit ZIKV internalization into the host cell [25]. Indeed, RNase protection, inactivation, binding, and internalization assays suggest that P. phillyreifolius-mediated inhibition of ZIKV was not associated with a loss of viral integrity but rather with a neutralization of ZIKV infectivity allowing attachment and preventing internalization of ZIKV particles in the host cell. Geraniin-mediated inhibition of ZIKV is different from that described previously where geraniin extracted from Nephelium lappaceum inhibits DENV-2 binding to the host cell [34].
Phyllanthus is one of the largest genera in the family Phyllanthaceae that comprises over 700 species distributed mainly in the tropics and subtropics and is in constant use in traditional medicine to treat diverse human diseases [48]. Phyllanthus species represent an extensive phytochemical diversity, e.g., tannins, terpenes, alkaloids, flavones, saponins, etc. These chemical compounds derived from Phyllanthus species exhibit antiviral effects against a broad spectrum of enveloped RNA and DNA viruses [49][50][51][52][53][54][55]. The results presented in this study underscore endemic medicinal plants from the Mascarene islands as a promising source of antivirals and add Phyllanthus phillyreifolius to the group of Phyllanthus species exhibiting antiviral effects against medically relevant flavivirus.
Importantly, in addition to this antiviral activity, we demonstrated that P. phillyreifolius extract, as well as its major compound geraniin, do not show acute toxicity in vivo using a zebrafish model, relevant for drug toxicity assessment [43,44,[56][57][58]. Our zebrafish assay showed that neither the plant extract nor its major compound geraniin impact fish behavior (feeding and locomotion), and no sign of stress was reported during the 5 days of investigation following drug exposure. The absence of striking behavioral effects at the effective antiviral concentration of geraniin on fish behavior (feeding, swimming, locomotor activity, stress), suggests that this concentration should be safe for further pre-clinical studies. These innocuity and non-deleterious effect at effective antiviral doses further indicate that the medicinal plant P. phillyreifolius is a potential source for the development of natural and safe antiviral drugs to fight ZIKV and DENV infections.

Extraction and Phytochemical Characterization of P. phillyreifolius Extract
The leaves of P. phillyreifolius were collected in the southwest of Reunion Island in November 2013 and immediately subjected to drying overnight at 37 • C. 5 g of dried and milled (to 1 mm particle size) plant material was extracted twice by stirring with 100 mL ethanol:water (70:30, v/v) at room temperature for 1 h, the extract filtered over Whatman No.1 filter paper and combined. We selected a traditional extraction method, which has been widely used for medicinal herbs. The method was proven to guarantee the effective and safe recovery of sensitive bioactive molecules. After extraction, ethanol was removed in a rotary evaporator (Büchi, Flawil, Switzerland), while residual water was freeze dried. The yield of obtained extract was 33.63 ± 1.15%. Dry extract was stored in a freezer prior to analysis.
The component analysis of extract was performed on an UPLC system equipped with a photodiode array (PDA) detector (Waters, Milford, MA, USA) according to the procedure previously described by [32]. The separation was carried out on Acquity BEH, C18 column (100 mm × 2.1 mm, 1.7 µm) at a column temperature of 40 • C. The mobile phase consisted of solvents 0.1% formic acid in ultrapure water (A) and 100% acetonitrile (B) with a linear gradient programmed as follows: 0.0-14 min, 5% B; 15-17 min, 100% B; 18 min, 5% B. The flow rate was 0.4 mL/min and the injection volume 1 µL.
The UPLC system was coupled to quadrupole-time of flight mass spectrometer (Q-TOF) equipped with an electrospray ionization probe and controlled by HyStar software (Bruker Daltonic, Bremen, Germany). MS data were recorded in ESI negative ionization mode over a mass range from m/z 80 to 1200, the capillary voltage was maintained at +4000 V. The peaks were identified by comparing their retention times and parent ions with external standards, references, and commercial databases.
The quantification of geraniin content was performed using Acquity UPLCTM H-Class equipped with Xevo TQ-S tandem quadrupole mass spectrometer (Waters, Milford, MA) as reported elsewhere [32]. Chromatographic separation was performed using the same column and solvents as described above with a linear gradient programmed as: 0.0-7 min, 5% B; 8-9 min, 50,7% B; 10-11 min, 100% B; 12-20 min, 5% B. The flow rate was 0.4 mL/min, and the sample injection volume was 5 µL. MS detection was achieved in the single-ion-monitoring (SIM) mode. The m/z values and dwell times of geraniin were set as 951.1957 m/z at 0.050 s. The concentration of geraniin in extract was calculated from calibration curve prepared using geraniin standard at concentrations of 0.05-50 µg/mL (y = 6158x + 4127; R 2 = 0.9986). Geraniin concentration in extract was 327 mg/g DWE. To evaluate the recovery of geraniin from 1 g of dried plant, this concentration was recalculated to g of dry weight of plant (DWP) and resulted 110 mg/g DWP.

MTT Assay
Two-fold dilutions of plant extract and geraniin ranging from 1000 µg/mL to 2 µg/mL were used to treat A549 cells at a density of 1.5 × 10 4 cells per well cultured in 96-well culture plates. Cells were rinsed with PBS 1X and 120 µL of culture medium mixed with 5 mg/mL MTT (3-[4,5-dimethylthiazol-2-yl]-2,5diphenyltetrazolium bromide) solution was added 24 h post incubation at 37 • C. The cells were re-incubated for 2 h at 37 • C. MTT medium was then removed, and the formazan crystals were solubilized with 50 µL of DMSO. Absorbance was measured at 570 nm with a background subtraction at 690 nm. The CC 50 was determined using a nonlinear regression on Graphpad prism software.

Flow Cytometry Assay
Cells were rinsed twice with PBS1X, trypsinated with 30 µL of trypsin/EDTA, and then fixed with 3.7% PFA in MEM for 15 min. The cells were then subjected to a flow cytometric analysis using Cytoflex (Beckman Coulter, Brea, CA, USA). Results were analyzed using cytExpert software (Brea, CA, USA).

RT-qPCR
Total RNA including genomic viral RNA was extracted from cells with RNeasy kit (Qiagen, Hilden, Germany) and reverse transcribed using E reverse primer (5'-TTCACCTTGTGTTGGGC-3') and M-MLV reverse transcriptase (Life Technologies, Villebon-sur-Yvette, France) at 42 • C for 50 min. Quantitative PCR was performed on a CFX96 Real-Time PCR Detection System (Applied Biosystems, Life Technologies, Villebon-sur-Yvette, France). Viral genomic cDNA was amplified using 0.2 µM of each primer and GoTaq Master Mix (Promega, Charbonnières-les-bains, France). For each single-well amplification reaction, a threshold cycle (Ct) was calculated using the CFX96 program (Bio-Rad) in the exponential phase of amplification. A synthetic gene coding for nucleotides 954 to 1306 of the MR766 strain (GenBank: LC002520) cloned in the pUC57 plasmid was used as template to generate a standard curve, which then served to make an absolute quantitation of bound viruses.

Plaque Forming Assay
Plaque forming unit assay was used to quantify virus infectious particles. Vero cells were seeded the previous day in 24-well culture plates at a density of 7 × 10 4 cells per well. Cells were infected by 0.15 mL of tenfold dilutions of supernatant. After 2 h of incubation at 37 • C, 0.2 mL of culture medium supplemented with 5% fetal bovine serum (FBS) and 0.8% carboxymethylcellulose sodium salt (Sigma-Aldrich) was added, and the incubation was extended for 96 h at 37 • C. Cells were rinsed with PBS 1X, fixed with PFA 3.7 % in PBS 1X, and stained with 0.5% crystal violet (Sigma-Aldrich, Saint-Quentin-Fallavier, France) diluted in 20% ethanol. Plaques were counted and expressed as plaque-forming unit per mL (PFU/mL)

Immunofluorescence Assay
Cells were rinsed twice with PBS1X, fixed with PFA, 3.7% in PBS1X, and permeabilized for 5 min (PBS 1X 0.15% Triton X-100). Cells were then stained 1h at room temperature in the dark for ZIKV E protein using 4G2-Alexa 594 (1:1000 in PBS-BSA 2%). DAPI staining was used to label cells nuclei. Coverslips were mounted in Vectashield, and the fluorescence was observed using Nikon Eclipse E2000-U microscope. Hamamatsu ORCA-ER camera and NIS-Element AR (Nikon) imaging software were used to capture images.

Foci-Forming Immunodetection Assay
C6/36 cells were seeded at a density of 1 × 10 5 in 24-well plates and incubated overnight at 37 • C. Tenfold serial dilutions of supernatant were prepared in duplicate in culture medium and 0.4 mL of each dilution was added to cells for 90 min. Then the inoculum was removed and a CMC media (L-15 supplemented with 10% FBS, 0.52% tryptose, 50 mg/mL gentamicin, 1.6% carboxymethylcellulose) was added. After seven days of incubation, immunostaining was performed using the mouse monoclonal antibody 4G2 followed by goat-antimouse immunoglobulin conjugated to alkaline phosphatase (Promega, Madison, WI, USA). A solution of NBT (nitroblue tetrazolium chloride) and BCIP (5-bromo-4-chloro-39-indolyphosphate p-toluidine salt) (Promega, Madison, WI, USA) was used as substrate to detected antibodies. Foci were counted and expressed as FFU/mL.

Virus Binding Assay
A549 cells were seeded in 12-well plates at a density of 2 × 10 5 cell per well. Cells were incubated with ZIKV at MOI of 1 in the presence or absence of plant extract or geraniin for 1 h at 4 • C. The inoculum was then removed, and cells were washed with cold MEM supplemented with 2% FBS. Samples were then submitted to RT-qPCR.

Internalization Assay
A549 cells were seeded in 24-well plates at a density of 1.5 × 10 5 cells per well. Cells were incubated with ZIKV GFP MOI of 1 for 1 h at 4 • C. Cells were then shifted to 37 • C in the presence or absence of plant extract or geraniin. The latter have been added at different time points, post temperature shift, covering the entry step of ZIKV infection. Cells were further incubated until 24 h at 37 • C. Cells were fixed with PFA 3.7% and then submitted to flow cytometry assay.

Virus Inactivation Assay
ZIKV GFP free particles (5 × 10 5 PFU) were incubated with the plant extract or geraniin for 2 h at 37 • C. As control, ZIKV GFP was incubated with MEM supplemented with 0.2% of DMSO. The mixture was diluted to 40-fold prior to infection and then inoculated on A549 cells. The supernatant was discarded after 2 h of incubation at 37 • C. Cells were washed twice with PBS1X and then incubated for 22 h at 37 • C before being subjected to cytometry assay.

Fish Maintenance, Intraperitoneal Injection, and Locomotor Activity Recording
Adult wildtype zebrafish (Danio rerio; 3-6 months male) were maintained under standard conditions of temperature (28 • C), photoperiod (14/10 h light/dark), and conductivity (400 µS). Every day, fish were fed 3 times with commercially available food from Planktovie (Gemma Micro ZF 300). For intraperitoneal injections, fish were anesthetized with 0.02% tricaine (MS-222; REF: A5040, Sigma-Aldrich) and injected with the respective vehicle (1X PBS, CTRL), Phyllanthus phillyreifolius extract (375 microg.g −1 of body weight) and its main active compound Geraniin (300 microg.g −1 of body weight). After injection, fish were immediately placed back in fish water and carefully observed for detecting any striking signs of stress, locomotor activity, and feeding behavior issues. At 1 and 5 days post injection (dpi), 3 CTRL and geraniin-injected fish were monitored for locomotor activity using the ZebraCube equipment (Viewpoint). Individual fish were placed in separate tanks containing 250 mL of water within the ZebraCube. A separation was placed between the CTRL and geraniin tanks in order to avoid visual interaction between the fish from the two groups. Before monitoring the locomotion, fish were adapted to their new tank for 10 min. Then, the locomotor activity was recorded for 10 min defining inactivity (<4 mm/s), small activity (4-8 mm/s), or high activity (>8 mm/s) states. A total number of 3 CTRL fish versus 3 geraniin fish were subjected to behavioral analysis at 1 dpi, and 5 CTRL fish versus 5 geraniin fish at 5 dpi. All animal experiments were conducted in accordance with the French and European Community Guidelines for the Use of Animals in Research (86/609/EEC and 2010/63/EU) and approved by the local Ethics Committee for animal experimentation of CYROI (APAFIS #2019052910002738_v4). At the end of the procedure, the animals were sacrificed with an overdose of tricaine.

Data Analysis
Comparison between different concentrations was done by a one-way ANOVA test. All values were expressed as mean ± SD of at least three independent experiments. All statistical tests were done using the software Graph-Pad Prism (version 8.0; La Jola, CA, USA). Values of p < 0.05 were considered statistically significant for a Dunnett's multiple comparisons test. Degrees of significance are indicated on the figure as follow: ** p < 0.01; *** p < 0.001, n.s. = not significant.