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Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of Zanthoxylum nitidum Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MS

1
Key Laboratory of Ministry of Education, Research Center of Chinese Herbal Resources and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
2
School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
*
Authors to whom correspondence should be addressed.
Molecules 2019, 24(3), 585; https://doi.org/10.3390/molecules24030585
Received: 25 December 2018 / Revised: 21 January 2019 / Accepted: 1 February 2019 / Published: 7 February 2019
(This article belongs to the Section Analytical Chemistry)
Zanthoxylum nitidum (Roxb.) DC (Rutaceae), called as “liangmianzhen” in China, is well known for its anti-inflammation and analgesic effect. Alkaloids are its main active constituents. However, little has been known about the absorption of main alkaloids in vivo. In this study, an ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry was employed for identification of absorbed alkaloids in rats after oral administration of Z. nitidum decoction. By analyzing the fragmentation patterns, a total of nineteen alkaloids were exactly or tentatively identified in rat plasma after treatment, of which magnoflorine, α-allocryptopine, and skimmianine are dominant. Moreover, a high performance liquid chromatography coupled mass spectrometry method was developed for simultaneous quantification of magnoflorine, α-allocryptopine, and skimmianine, and successfully applied to pharmacokinetic study in rats after oral administration of Z. nitidum decoction. The research would contribute to comprehensive understanding of the material basis and function mechanism of Z. nitidum decoction. View Full-Text
Keywords: Zanthoxylum nitidum; UPLC-Q-TOF-MS/MS; pharmacokinetic study; HPLC-MS/MS; magnoflorine Zanthoxylum nitidum; UPLC-Q-TOF-MS/MS; pharmacokinetic study; HPLC-MS/MS; magnoflorine
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    Description: The extract ion chromatograms (EICs) and MS2 spectra of 19 components in rat plasma after oral administration of Z. nitidum decoction
MDPI and ACS Style

Huang, A.; Chi, Y.; Liu, J.; Wang, M.; Qin, J.; Ou, L.; Chen, W.; Zhao, Z.; Zhan, R.; Xu, H. Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of Zanthoxylum nitidum Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MS. Molecules 2019, 24, 585. https://doi.org/10.3390/molecules24030585

AMA Style

Huang A, Chi Y, Liu J, Wang M, Qin J, Ou L, Chen W, Zhao Z, Zhan R, Xu H. Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of Zanthoxylum nitidum Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MS. Molecules. 2019; 24(3):585. https://doi.org/10.3390/molecules24030585

Chicago/Turabian Style

Huang, Aihua, Yuguang Chi, Jiawei Liu, Mincun Wang, Jialiang Qin, Lijuan Ou, Weiwen Chen, Zhongxiang Zhao, Ruoting Zhan, and Hui Xu. 2019. "Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of Zanthoxylum nitidum Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MS" Molecules 24, no. 3: 585. https://doi.org/10.3390/molecules24030585

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