Appraisal of Comparative Therapeutic Potential of Undoped and Nitrogen-Doped Titanium Dioxide Nanoparticles

Nitrogen-doped and undoped titanium dioxide nanoparticles were successfully fabricated by simple chemical method and characterized using x-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive x-ray (EDX), and transmission electron microscopy (TEM) techniques. The reduction in crystalline size of TiO2 nanoparticles (from 20–25 nm to 10–15 nm) was observed by TEM after doping with N. Antibacterial, antifungal, antioxidant, antidiabetic, protein kinase inhibition and cytotoxic properties were assessed in vitro to compare the therapeutic potential of both kinds of TiO2 nanoparticles. All biological activities depicted significant enhancement as a result of addition of N as doping agent to TiO2 nanoparticles. Klebsiella pneumoniae has been illuminated to be the most susceptible bacterial strain out of various Gram-positive and Gram-negative isolates of bacteria used in this study. Good fungicidal activity has been revealed against Aspergillus flavus. 38.2% of antidiabetic activity and 80% of cytotoxicity has been elucidated by N-doped TiO2 nanoparticles towards alpha-amylase enzyme and Artemia salina (brine shrimps), respectively. Moreover, notable protein kinase inhibition against Streptomyces and antioxidant effect including reducing power and % inhibition of DPPH has been demonstrated. This investigation unveils the more effective nature of N-doped TiO2 nanoparticles in comparison to undoped TiO2 nanoparticles indicated by various biological tests. Hence, N-doped TiO2 nanoparticles have more potential to be employed in biomedicine for the cure of numerous infections.


Introduction
Biomedical applications of metallic oxide nanoparticles are recently considered an interesting avenue of research regarding nano-biotechnology. Nanoparticles possess unique physio-chemical, optical and electro-magnetic properties that make them appropriate tools for various applications in the field of materials science and pharmacy [1,2]. Co-precipitation is the most adaptive method for preparation of metallic oxide nanoparticles because of its reproducibility and cheapness [3]. Titanium dioxide (TiO 2 ) nanoparticles have been vastly synthesized by different chemical routes. Doping prevents aggregation between nanoparticles ultimately leading to their stability, longevity as well as intensified reactivity [4]. Hence, TiO 2 nanoparticles have been doped with Ni, Cu, Fe, Mo, N and other metals using hydrolysis, precipitation, sol-gel and other methods for fabrication in the past [5][6][7][8][9][10][11]. Metal-doped TiO 2 nanoparticles serve many environmental remediation purposes like removal of organic dyes and wastes/pollutants owing to their tremendous photocatalytic activity [12]. In context of their role in biomedicine, antibacterial activity of Mn-doped TiO 2 and Nd-doped TiO 2 nanoparticles has been documented [13,14]. TiO 2 nanoparticles have been proven important skin protecting agents against UV radiation and used in sunscreens/cosmetics as reported by Viana et al. [15]. Cytotoxicity of TiO 2 nanoparticles doped with Ag, Cu, Fe has been validated against human cancer cell lines and hence these nanoparticles have been successfully declared anti-cancerous materials [16][17][18].
Regarding bioremediation studies, the photocatalytic property of N-doped TiO 2 nanoparticles for air and water purification was exclusively described [19][20][21]. Talking about biomedical properties, Zane et al. [22] reported the biocompatibility and antibacterial activity of N-doped TiO 2 nanoparticles and its applications in dental resin formulations. Moreover, the assessment of orthodontic brackets coated with N-doped TiO 2 nanoparticles against Streptococcus mutans was illuminated by Salehi et al. [23]. Atchudan et al. [24] explained the applications of N-doped TiO 2 nanoparticles in cell imaging due to their good biocompatibility. Li et al. [25] studied the photokilling effect of N-doped TiO 2 nanoparticles on cancer cells.
The purpose of the present study was to complement the vacuum in literature regarding exploration of comprehensive biological potential of N-doped and undoped TiO 2 nanoparticles. According to our knowledge, this is the first report covering multifunctional role of bare TiO 2 nanoparticles and N-doped TiO 2 nanoparticles in biomedicine. Antibacterial, antifungal, antioxidant, antidiabetic, protein kinase inhibition and cytotoxicity assays have been carried out under in vitro conditions. Comparative assessment of undoped and N-doped TiO 2 nanoparticles has been performed to exploit the effectivity of these nanoparticles to be used as drug-loading agents or carriers in nanomedicine for the cure of countless fatal diseases disrupting human health.

X-Ray Diffraction (XRD)
XRD data confirmed the nanocrystalline structure of TiO 2 nanoparticles and N-doped TiO 2 nanoparticles as evident from the sharpness of peaks obtained in Figure 1. The structure of TiO 2 nanoparticles is tetragonal.
Molecules 2019, 24, x 2 of 19 prevents aggregation between nanoparticles ultimately leading to their stability, longevity as well as intensified reactivity [4]. Hence, TiO2 nanoparticles have been doped with Ni, Cu, Fe, Mo, N and other metals using hydrolysis, precipitation, sol-gel and other methods for fabrication in the past [5][6][7][8][9][10][11]. Metal-doped TiO2 nanoparticles serve many environmental remediation purposes like removal of organic dyes and wastes/pollutants owing to their tremendous photocatalytic activity [12]. In context of their role in biomedicine, antibacterial activity of Mn-doped TiO2 and Nd-doped TiO2 nanoparticles has been documented [13,14]. TiO2 nanoparticles have been proven important skin protecting agents against UV radiation and used in sunscreens/cosmetics as reported by Viana et al. [15]. Cytotoxicity of TiO2 nanoparticles doped with Ag, Cu, Fe has been validated against human cancer cell lines and hence these nanoparticles have been successfully declared anti-cancerous materials [16][17][18].
Regarding bioremediation studies, the photocatalytic property of N-doped TiO2 nanoparticles for air and water purification was exclusively described [19][20][21]. Talking about biomedical properties, Zane et al. [22] reported the biocompatibility and antibacterial activity of N-doped TiO2 nanoparticles and its applications in dental resin formulations. Moreover, the assessment of orthodontic brackets coated with N-doped TiO2 nanoparticles against Streptococcus mutans was illuminated by Salehi et al. [23]. Atchudan et al. [24] explained the applications of N-doped TiO2 nanoparticles in cell imaging due to their good biocompatibility. Li et al. [25] studied the photokilling effect of N-doped TiO2 nanoparticles on cancer cells.
The purpose of the present study was to complement the vacuum in literature regarding exploration of comprehensive biological potential of N-doped and undoped TiO2 nanoparticles. According to our knowledge, this is the first report covering multifunctional role of bare TiO2 nanoparticles and N-doped TiO2 nanoparticles in biomedicine. Antibacterial, antifungal, antioxidant, antidiabetic, protein kinase inhibition and cytotoxicity assays have been carried out under in vitro conditions. Comparative assessment of undoped and N-doped TiO2 nanoparticles has been performed to exploit the effectivity of these nanoparticles to be used as drug-loading agents or carriers in nanomedicine for the cure of countless fatal diseases disrupting human health.

X-Ray Diffraction (XRD)
XRD data confirmed the nanocrystalline structure of TiO2 nanoparticles and N-doped TiO2 nanoparticles as evident from the sharpness of peaks obtained in Figure 1. The structure of TiO2 nanoparticles is tetragonal. Later on, the size of nanoparticles was measured from the following Scherrer's formula: Where λ indicates the wavelength of X-rays, β is the full width at half maximum of peaks in radians, and θB is the Bragg's angle. The size of TiO 2 nanoparticles and N-doped TiO 2 nanoparticles was found to be 25 nm and 17.5 nm, respectively.

Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray (EDX)
The SEM micrographs in Figure 2 reveal spherical and coarse shape of TiO 2 nanoparticles and N-doped TiO 2 nanoparticles. However, morphology of TiO 2 nanoparticles indicate more aggregation while crystals of N-doped TiO 2 nanoparticles are aggregated less, showing better clarity of shape. Later on, the size of nanoparticles was measured from the following Scherrer's formula: Where λ indicates the wavelength of X-rays, β is the full width at half maximum of peaks in radians, and θB is the Bragg's angle. The size of TiO2 nanoparticles and N-doped TiO2 nanoparticles was found to be 25 nm and 17.5 nm, respectively.

Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray (EDX)
The SEM micrographs in Figure 2 reveal spherical and coarse shape of TiO2 nanoparticles and N-doped TiO2 nanoparticles. However, morphology of TiO2 nanoparticles indicate more aggregation while crystals of N-doped TiO2 nanoparticles are aggregated less, showing better clarity of shape. The EDX data in Figure 3 demonstrates that TiO2 nanoparticles were composed of only titanium and oxygen while N-doped TiO2 nanoparticles also had nitrogen found on the surface of TiO2. No other impurity was detected and these nanoparticles were found stoichiometric. Our results are concordant with the previous finding [26]. The SEM and EDX results were coinciding with XRD results as they also declared phase purity of TiO2 nanoparticles and N-doped TiO2 nanoparticles. The EDX data in Figure 3 demonstrates that TiO 2 nanoparticles were composed of only titanium and oxygen while N-doped TiO 2 nanoparticles also had nitrogen found on the surface of TiO 2 . No other impurity was detected and these nanoparticles were found stoichiometric. Our results are concordant with the previous finding [26]. The SEM and EDX results were coinciding with XRD results as they also declared phase purity of TiO 2 nanoparticles and N-doped TiO 2 nanoparticles.

Antibacterial Activity
Both bare TiO2 nanoparticles and N-doped TiO2 nanoparticles were tested for their antibacterial activity and found to be potent inhibitors of Gram-positive and Gram-negative bacteria as illustrated

Antibacterial Activity
Both bare TiO2 nanoparticles and N-doped TiO2 nanoparticles were tested for their antibacterial activity and found to be potent inhibitors of Gram-positive and Gram-negative bacteria as illustrated

Antibacterial Activity
Both bare TiO 2 nanoparticles and N-doped TiO 2 nanoparticles were tested for their antibacterial activity and found to be potent inhibitors of Gram-positive and Gram-negative bacteria as illustrated by Figures 5 and 6. The literature has also documented TiO 2 nanoparticles to be effective antibacterial agents [27][28][29][30][31]. There is difference in bacterial inhibition of different strains because of different functional groups present on their surface [4]. The positively charged nanoparticles and negatively charged bacterial surface leads to electrostatic interaction and hence, bactericidal activity by the formation of reactive oxygen species (ROS), that causes cell lethality after malfunctioning of bacterial synthetic machinery [31][32][33][34]. The mechanism for bactericidal activity of TiO 2 nanoparticles has been shown in Figure 7. by Figures 5 and 6. The literature has also documented TiO2 nanoparticles to be effective antibacterial agents [27][28][29][30][31]. There is difference in bacterial inhibition of different strains because of different functional groups present on their surface [4]. The positively charged nanoparticles and negatively charged bacterial surface leads to electrostatic interaction and hence, bactericidal activity by the formation of reactive oxygen species (ROS), that causes cell lethality after malfunctioning of bacterial synthetic machinery [31][32][33][34]. The mechanism for bactericidal activity of TiO2 nanoparticles has been shown in Figure 7. The antibacterial activity against Gram-negative bacteria (Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and resistant Pneumococcal aeruginosa) was more pronounced as compared to Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, methicillin resistant Staphylococcus aureus (MRSA) and resistant Streptococcus haemoliticus). The difference in cell wall composition and thickness of Gram-positive and Gram-negative bacteria might account for the variation in biological potential [4,35]. Furthermore, in the current scenario, it is believed that TiO2 nanoparticles more bonding, and after translocation into the cytoplasm and nucleus, result in oxidative damage by excessive ROS production and eventual mortality. Therefore, Gram-negative bacteria are more vulnerable to TiO2 nanoparticles than their Gram-positive complement. Recently, Ripolles-Avila et al. [36] performed antibacterial studies of TiO2 nanoparticles and observed no significant difference in their behavior towards Gram-positive and Gram-negative bacteria.  The antibacterial activity against Gram-negative bacteria (Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and resistant Pneumococcal aeruginosa) was more pronounced as compared to Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, methicillin resistant Staphylococcus aureus (MRSA) and resistant Streptococcus haemoliticus). The difference in cell wall composition and thickness of Gram-positive and Gram-negative bacteria might account for the variation in biological potential [4,35]. Furthermore, in the current scenario, it is believed that TiO 2 nanoparticles more effectively permeate Gram-negative bacterial cell wall because of its lesser thickness via chemical bonding, and after translocation into the cytoplasm and nucleus, result in oxidative damage by excessive ROS production and eventual mortality. Therefore, Gram-negative bacteria are more vulnerable to TiO 2 nanoparticles than their Gram-positive complement. Recently, Ripolles-Avila et al. [36] performed antibacterial studies of TiO 2 nanoparticles and observed no significant difference in their behavior towards Gram-positive and Gram-negative bacteria. The impact of doping agent was also prominent since the N-doped TiO2 nanoparticles showed more distinct bactericidal effect against all bacterial strains in comparison to undoped TiO2 nanoparticles. The highest antibacterial activity was elucidated by N-doped TiO2 nanoparticles against Klebsiella pneumoniae (zone of inhibition 17 mm) in this investigation. It is believed that doping causes reduction in nanoparticles' size, their higher surface area, and ultimately more significant antibacterial activity [34,37]. Moreover, it has been proposed that enhanced antibacterial activity of N-doped TiO2 nanoparticles is due to the synergistic effect of N and TiO2 [4,35].

Antifungal Activity
The TiO2 nanoparticles tested for their antifungal potential against Fusarium solani, Aspergillus flavus, Aspergillus fumigatus and Aspergillus niger were found mildly effective against only Aspergillus flavus (zone of inhibition 7 mm and 11 mm for TiO2 nanoparticles and N-doped TiO2 nanoparticles, respectively) as evident from Figures 8 and 9. Our study supports the previous report showing antifungal nature of TiO2 nanoparticles against Candida albicans [38]. The mechanism of fungicidal activity is not explicitly understood. However, it is thought to involve generation of ROS and oxidative deterioration causing cellular lethality [32,38]. The impact of doping agent was also prominent since the N-doped TiO 2 nanoparticles showed more distinct bactericidal effect against all bacterial strains in comparison to undoped TiO 2 nanoparticles. The highest antibacterial activity was elucidated by N-doped TiO 2 nanoparticles against Klebsiella pneumoniae (zone of inhibition 17 mm) in this investigation. It is believed that doping causes reduction in nanoparticles' size, their higher surface area, and ultimately more significant antibacterial activity [34,37]. Moreover, it has been proposed that enhanced antibacterial activity of N-doped TiO 2 nanoparticles is due to the synergistic effect of N and TiO 2 [4,35].

Antifungal Activity
The TiO 2 nanoparticles tested for their antifungal potential against Fusarium solani, Aspergillus flavus, Aspergillus fumigatus and Aspergillus niger were found mildly effective against only Aspergillus flavus (zone of inhibition 7 mm and 11 mm for TiO 2 nanoparticles and N-doped TiO 2 nanoparticles, respectively) as evident from Figures 8 and 9. Our study supports the previous report showing antifungal nature of TiO 2 nanoparticles against Candida albicans [38]. The mechanism of fungicidal activity is not explicitly understood. However, it is thought to involve generation of ROS and oxidative deterioration causing cellular lethality [32,38].

Antioxidant Activity
All kinds of antioxidant activities, i.e., total antioxidant capacity (TAC), total reducing power (TRP) and % DPPH inhibition have been observed by both undoped TiO 2 nanoparticles and N-doped TiO 2 nanoparticles. Prominent antioxidation potential has been documented by the previous studies [27,29,30,39]. However, the activities shown by N-doped TiO 2 nanoparticles are significantly higher than undoped TiO 2 nanoparticles as depicted by Figure 10. A significant elevation in all activities in N-doped TiO 2 nanoparticles is believed to be due to the addition of doping agent which reduces size of TiO 2 nanoparticles and enhances their reactivity [4,35]. All kinds of antioxidant activities, i.e., total antioxidant capacity (TAC), total reducing power (TRP) and % DPPH inhibition have been observed by both undoped TiO2 nanoparticles and N-doped TiO2 nanoparticles. Prominent antioxidation potential has been documented by the previous studies [27,29,30,39]. However, the activities shown by N-doped TiO2 nanoparticles are significantly higher than undoped TiO2 nanoparticles as depicted by Figure 10. A significant elevation in all activities in N-doped TiO2 nanoparticles is believed to be due to the addition of doping agent which reduces size of TiO2 nanoparticles and enhances their reactivity [4,35].

Antidiabetic Activity
The results of α-amylase inhibition as shown in Figure 11 demonstrate undoped TiO2 nanoparticles and N-doped TiO2 nanoparticles to be the potent candidates against diabetes mellitus. Previously, Stevia rebaudiana loaded TiO2 nanoparticles have been proved potential antidiabetic agents via in vivo studies performed in rats [40]. N-doped TiO2 nanoparticles possess more significant α-amylase inhibition (38.2%) as compared to bare TiO2 nanoparticles (24.8%). The enlargement of surface area by size reduction of TiO2 nanoparticles results in increase of chemical bonding of surface atoms or molecules which paves the way for enhancement of antidiabetic activity in N-doped TiO2 nanoparticles [4,35].

Antidiabetic Activity
The results of α-amylase inhibition as shown in Figure 11 demonstrate undoped TiO 2 nanoparticles and N-doped TiO 2 nanoparticles to be the potent candidates against diabetes mellitus. Previously, Stevia rebaudiana loaded TiO 2 nanoparticles have been proved potential antidiabetic agents via in vivo studies performed in rats [40]. N-doped TiO 2 nanoparticles possess more significant α-amylase inhibition (38.2%) as compared to bare TiO 2 nanoparticles (24.8%). The enlargement of surface area by size reduction of TiO 2 nanoparticles results in increase of chemical bonding of surface atoms or molecules which paves the way for enhancement of antidiabetic activity in N-doped TiO 2 nanoparticles [4,35].

Protein Kinase Inhibition Activity
The premier protein kinase inhibition was achieved by undoped TiO 2 nanoparticles (inhibition zone 12 mm) and N-doped TiO 2 nanoparticles (inhibition zone 16 mm) as deciphered by Figures 12 and 13. It has been explicitly found that the highest protein kinase inhibition activity was demonstrated by N-doped TiO 2 nanoparticles in comparison to their bare counterpart which might be due to the enhanced surface area of TiO 2 nanoparticles after doping with N [4]. Protein kinase inhibition is the preliminary anticancer assay and previous reports have documented very good anticancer activity of TiO 2 nanoparticles [41][42][43][44].

Protein Kinase Inhibition Activity
The premier protein kinase inhibition was achieved by undoped TiO2 nanoparticles (inhibition zone 12 mm) and N-doped TiO2 nanoparticles (inhibition zone 16 mm) as deciphered by Figures 12  and 13. It has been explicitly found that the highest protein kinase inhibition activity was demonstrated by N-doped TiO2 nanoparticles in comparison to their bare counterpart which might be due to the enhanced surface area of TiO2 nanoparticles after doping with N [4]. Protein kinase inhibition is the preliminary anticancer assay and previous reports have documented very good anticancer activity of TiO2 nanoparticles [41][42][43][44].

Cytotoxic Activity
Brine shrimp lethality assay was an appropriate preliminary test for screening of cytotoxicity of undoped TiO2 nanoparticles and N-doped TiO2 nanoparticles. Figure 14 shows that lesser

Cytotoxic Activity
Brine shrimp lethality assay was an appropriate preliminary test for screening of cytotoxicity of undoped TiO 2 nanoparticles and N-doped TiO 2 nanoparticles. Figure 14 shows that lesser cytotoxicity was obtained by bare TiO 2 nanoparticles (70% of mortality at 50 µg/mL) as compared to N-doped TiO 2 nanoparticles (80% of mortality at 50 µg/mL). Previous literature supports our findings by suggesting that TiO 2 nanoparticles are cytotoxic to various human cell lines and doping further improves the cytotoxic status of these nanoparticles [16]. He et al. [45] elucidated concentration-dependent cytotoxic activity of TiO 2 nanoparticles in human prostate cancer cell lines. Chellappa et al. [46], Ahamed et al. [15], Rahmani Kukia et al. [47] and Koca and Duman, [48] also reported the in vitro and in vivo cytotoxicity of TiO 2 nanoparticles. The mechanism behind cytotoxicity might involve internalization of TiO 2 nanoparticles into cytoplasm resulting in generation of ROS by oxidative stress and alteration in cellular redox state. It eventually causes mitochondrial damage and cell death by apoptosis [49][50][51]. cytotoxicity was obtained by bare TiO2 nanoparticles (70% of mortality at 50 µg/mL) as compared to N-doped TiO2 nanoparticles (80% of mortality at 50 µg/mL). Previous literature supports our findings by suggesting that TiO2 nanoparticles are cytotoxic to various human cell lines and doping further improves the cytotoxic status of these nanoparticles [16]. He et al. [45] elucidated concentrationdependent cytotoxic activity of TiO2 nanoparticles in human prostate cancer cell lines. Chellappa et al. [46], Ahamed et al. [15], Rahmani Kukia et al. [47] and Koca and Duman, [48] also reported the in vitro and in vivo cytotoxicity of TiO2 nanoparticles. The mechanism behind cytotoxicity might involve internalization of TiO2 nanoparticles into cytoplasm resulting in generation of ROS by oxidative stress and alteration in cellular redox state. It eventually causes mitochondrial damage and cell death by apoptosis [49][50][51]. The overall results of TiO2 nanoparticles and N-doped TiO2 nanoparticles with respect to their characterization techniques and therapeutic potential have been listed in Table 1. The overall results of TiO 2 nanoparticles and N-doped TiO 2 nanoparticles with respect to their characterization techniques and therapeutic potential have been listed in Table 1.

Chemical Fabrication of TiO 2 Nanoparticles and N-Doped TiO 2 Nanoparticles
In brief, TiO 2 nanoparticles were synthesized by a facile chemical method of co-precipitation. The reagents used were purchased from Sigma-Aldrich (St. Louis, MO, USA) and included titanium isopropoxide (97%), isopropyl alcohol (≥98%), nitric acid (70%) and ethanol (96%). The procedure of Shajudheen et al. [52] was followed after some modifications. Synthesis of undoped TiO 2 nanoparticles involved addition of 100 mL isopropyl alcohol to 15 mL titanium isopropoxide under continuous stirring for 30 min. 10 mL deionized water for hydrolysis was later added dropwise until white precipitates were obtained that were filtered and washed with deionized water and ethanol thrice. The precipitates were dried at 100 • C and grinded to a fine powder. Finally, calcination was done at 800 • C for 4 h.
In case of N-doped TiO 2 nanoparticles synthesis, 15 mL nitric acid as a nitrogen source was added to titanium isopropoxide mixture and it was vigorously stirred until dissolved. Later on, deionized water was added followed by the procedure similar to undoped synthesis of TiO 2 nanoparticles. The shape and elemental composition of nanoparticles was revealed using scanning electron microscopy (SEM) and energy dispersive x-ray (EDX) spectroscopy. HITACHI S-4800 (HITACHI, Ibraraki, Japan) was used to determine SEM and EDX images.

Transmission Electron Microscopy (TEM)
The transmission electron microscopy (TEM) was executed with HITACHI H-7650 (HITACHI, Japan) in order to find out an exact size of nanoparticles.

Antibacterial Activity
The fabricated nanoparticles were subjected to screening for their antibacterial activity against various gram-positive and gram-negative bacterial strains. Agar disc diffusion method was employed for this purpose following the procedure of Haq et al. [53] after slight modifications. The Gram-positive bacterial isolates used were Bacillus subtilis, Staphylococcus aureus, methicillin resistant Staphylococcus aureus (MRSA) and resistant Streptococcus haemoliticus. Whereas, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and resistant Pneumococcal aeruginosa were the Gram-negative isolates of bacteria used for finding antibacterial potential. All of strains were allowed to grow on 2% nutrient agar (NA) at 25 • C and stored in refrigerator. Then, 100 µL of tryptic soy broth (TSB) was spread on agar medium in which bacterial colonies were grown. Stock solution of 1 mg/mL of nanoparticles was made in dimethyl sulfoxide (DMSO), from which 50 µg/mL of final concentration was made by taking out 50 µL of solution and loading on discs found on petri plates. The incubation of plates was done at 37 • C for 24 h. DMSO were used as negative control, whereas Roxithromycin and Cefixime were taken as positive controls. Vernier caliper was used to measure inhibition zones.

Antifungal Activity
Agar disc diffusion method was used by following the procedure of Akhtar et al. [54] after slight modifications to evaluate antifungal activity of nanoparticles. The fungal strains used were Fusarium solani, Aspergillus flavus, Aspergillus fumigatus and Aspergillus niger. All strains were preserved in refrigerator at 4 • C on sabouraud dextrose agar (SDA). After culturing of fungal strains on SDA, 50 µg/mL of nanoparticles solution was loaded on discs found on petri plates containing media. The prepared petri dishes were incubated at 28 • C for 72 h. DMSO and Clotrimazole were used as negative control and positive control, respectively. The zones of inhibition were measured with Vernier caliper.

Total Antioxidant Capacity (TAC)
TAC of nanoparticles was elucidated by some modifications in the method of Khan et al. [55]. 4mg/mL stock solution of nanoparticles was made from which 100 µL was taken out and mixed with 900 µL of reagent solutions (4 mM ammonium molybdate, 28 mM sodium phosphate and 0.6 M sulfuric acid). After incubation at 95 • C for 90 min, absorbance was taken at 695 nm using microplate reader. DMSO and Ascorbic acid were taken as negative control and positive control, respectively. The results were expressed as µg AA/mg.

Total Reducing Power (TRP)
TRP of nanoparticles was evaluated by some modifications in the method of Khan et al. [55]. From 4mg/mL stock nanoparticles solution, 100 µL was mixed with reagents (200 µL of phosphate buffer and 250 µL of 1% potassium ferricyanide). After incubation at 50 • C for 20 min, 200 µL of 10% trichloroacetic acid was added. Centrifugation (LB-5, Heraeus, Hanau, Germany) was carried out at 3000 rpm for 10 min. Later on, 150 µL of supernatant solution was taken and mixed with 50 µL of 0.1% ferric chloride solution. Finally, absorbance was measured at 630 nm using microplate reader. DMSO and Ascorbic acid were used as negative control and positive control, respectively. The results were expressed as µg AA/mg.

% DPPH Inhibition
Free radical scavenging activity of nanoparticles was determined using 2,2-diphenyl-1-picryl hydrazyl (DPPH) reagent by some modifications in the method of Khan et al. [55]. 10 µL of nanoparticles solution was taken from 4mg/mL stock solution and mixed with 190 µL of DPPH reagent. After incubation for 1 h in dark, absorbance was measured at 515 nm with microplate reader. DMSO and Ascorbic acid were used as negative control and positive control, respectively. % free radical scavenging or % inhibition of nanoparticles with DPPH was calculated using the following formula: % DPPH Inhibition = (1 − Abs/Abc) × 100 (2) where Abs is the absorbance of nanoparticles with DPPH reagent, and Abc is the absorbance of negative control.

Antidiabetic Activity
Antidiabetic activity or α-amylase inhibition was determined by a method described by [56] after slight modifications. It involved addition of 15 µL of phosphate buffer, 25 µL of α-amylase, 10 µL of nanoparticle solution and 40 µL of starch to the well of microtiter plate. The plate was incubated at 50 • C for 30 min. 20 µL of 1 M HCl and 90 µL of iodine solution were added thereafter. DMSO and Acarbose were taken as negative control and positive control, respectively. DMSO, starch and buffer were taken as blank. Microplate reader measured the absorbance at 540 nm.
% inhibition of α-amylase was calculated by the following formula: where ODx, ODy and ODz symbolize the absorbance of nanoparticles, negative control and blank, respectively.

Protein Kinase Inhibition Activity
The method established by Yao et al. [57] after slight modifications was utilized to determine preliminary antitumor activity of nanoparticles by means of protein kinase inhibition assay. It was based on the principle of inhibition in hyphae formation in Streptomyces whose mycelia fragments were allowed to disperse on the surface of ISP4 plates of agar media. 5 µL of nanoparticles solution was applied to these plates and placed for incubation for 72 h. The clear or bald zones appeared after incubation were measured using Vernier caliper. The bald zone more than 9 mm is considered significant and indication of restriction in bacterial hyphae formulation. DMSO and Surfactin were taken as negative control and positive control, respectively.

Cytotoxic Activity
Brine shrimp lethality test was performed to reveal the cytotoxicity of nanoparticles by a method of Apu et al. [58] after minor modifications. The Artemia salina (brine shrimp) eggs were hatched in a tank containing artificial sea water having incubation temperature of 30 • C. The tank was comprised of two unequal portions; the larger portion contained eggs and was sealed with aluminium foil, while the smaller portion had light source due to which the newly hatched larvae of shrimps gathered there within 1-2 days. The mature nauplii were shifted in a small beaker using Pasteur pipette. Different dilutions from stock solutions (4 mg/mL) of nanoparticles at concentrations of 50 µg/mL, 25 µg/mL and 12.5 µg/mL were tested for assessment of lethality. Each well of 96-well plate were filled with 10 nauplii and 150 µL of sea water in which the dilutions were poured that raised the final volume to 300 µL. The resulting solutions were analyzed for quantification of % of dead nauplii after 2 h of incubation period. DMSO and Doxorubicin were used as negative control and positive control, respectively. Table curve 2 D Ver. 4 software was used to calculate LC50 of nanoparticles.

Statistical Analysis
All tests were performed in triplicate and data were presented as mean ± standard deviation (SD) from at least three replicates.

Conclusions
Although there is abundant data available on the investigation of biological uses of TiO 2 nanoparticles, rare content is found regarding study of comparative effects of doping agent/surfactant on the biological nature of these nanoparticles. Therefore, this study was intended to estimate the comprehensive therapeutic potential of synthesized undoped and N-doped TiO 2 nanoparticles via different biological activities performed in vitro. All bioassays have proved the efficiency of N-doped TiO 2 nanoparticles and good/moderate biological activities have been achieved. In short, these nanoparticles are highlighted as tools for use in the treatment of various human diseases. However, further extensive attempts on cytotoxic and antitumor inquiry of these nanoparticles on the normal human cells are strongly recommended.