Bioactive Molecules and Their Mechanisms of Action

Chronic inflammation with a wide spectrum of connected diseases (e [...].

Cancer is the second leading cause of death, and accounted for an estimated 9.6 million deaths worldwide in 2018 (https://www.who.int/news-room/fact-sheets/detail/cancer). Many small chemical entities are already used clinically for conventional and targeted cancer therapy, and much more recent research studies focus on the dissection of novel antitumor effects of diverse molecules [18][19][20]. As shown in this special issue, Mrkvová et al. performed high throughput screening of 2448 compounds for activating tumor suppressor p53 in the tumor cell line A375 [21]. The authors found that benzimidazoles activated p53 by downregulating Mdm2 and MdmX, which could be promising in the treatment of cancers overexpressing negative regulators of p53 [21]. Proinflammatory M1 macrophages promote tumoricidal response and suppress tumor growth, whereas M2(-like) macrophages contribute to a tumor environment by pampering cancer cell proliferation and survival [22]. Regarding macrophage polarization, Wei et al. reported that a polysaccharide isolated from Radix Astragali shifted macrophage phenotype polarization to M1 via the notch signaling pathway to further incite cytotoxic activity of macrophages against cancer cells, as well as decreased tumor volume and tumor weight in vivo [23]. In addition, Ye et al. found that 4β-hydroxywithanolide E from Physalis peruviana exhibited anti-colorectal cancer activity in vitro and in vivo by attenuating the wingless-type MMTV integration site family (Wnt)/β-catenin signaling pathway [24]. Moreover, the study of Luo et al. showed that vicenin II, a flavonoid glycoside (extracted also from Dendrobium officinale), inhibited transforming growth factor (TGF)-β1-induced epithelial-mesenchymal transition in lung adenocarcinoma cells via the deactivation of TGF-β/Smad and phosphoinositide 3-kinases (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathways, showing potential anti-metastatic activity [25]. Finally, Sperlich et al. discovered that pseudopterosin, produced by the sea whip of the genus Antillogorgia, inhibited proliferation and 3D invasion in triple-negative breast cancer cells by agonizing glucocorticoid receptor-α, as well as blocked elevation of levels of cytokines in a co-culture of peripheral blood mononuclear cells and MDA-MB-231 cells [26].
CVDs are disorders of the heart and blood vessels many times connected with chronic inflammation (https://www.who.int/health-topics/cardiovascular-diseases/). A total of 17.9 million people die each year from CVDs, an estimated 31% of all deaths worldwide (https://www.who.int/health-topics/ cardiovascular-diseases/). Hypertension is a critical risk factor for the development of other CVDs and their complications. The study of Kim et al. indicated that Citrus unshiu peel contained both the contractile agonist synephrine and the anti-contractile substance nobiletin, which led to the abnormal vasoconstriction pattern exhibited by this herb [27].
Diabetes is a chronic metabolic disorder, which leads over time to serious hyperglycemia-induced damage to the heart, blood vessels, eyes, kidneys, and nerves (https://www.who.int/health-topics/ diabetes). The most common type is type 2 diabetes, which occurs when the body becomes resistant to or possesses a relative lack of insulin (https://www.who.int/health-topics/diabetes). As currently used anti-diabetic drugs have marked side effects, continuous efforts to identify and characterize new molecules with anti-diabetic action are ongoing [28,29]. In this special issue, Yang et al. reported that alkali extract from Amillariella mellea fruiting body, polysaccharide-enriched fraction, lowered fasting blood glucose and improved glucose intolerance and insulin resistance in type 2 diabetic rats [30].
Neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and multiple sclerosis are characterized by neuronal cell death, caused in part by an inflammatory process, mitochondrial alterations, and an elevation of oxidative stress [31,32]. Namsi et al. reported that an endogenous substance octadecaneuropeptide (ODN) induced N2a cell differentiation by the activation of the protein kinase A (PKA)/phospholipase C (PLC)/protein kinase C (PKC)/mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK)-dependent signaling pathway, suggesting that ODN is a potent neuroprotective agent [32].
Osteoporosis is a highly prevalent bone disease caused by an inappropriate rate of bone resorption compared with bone formation, and the characterization of novel compounds with anti-osteoporotic potential is of high interest [33][34][35][36]. Yodthong et al. reported that L-quebrachitol promoted the proliferation, differentiation, and mineralization of pre-osteoblastic MC3T3-E1 cells by triggering the bone morphogenetic protein-2 (BMP-2)-response, as well as the runt-related transcription factor-2 (Runx2), mitogen-activated protein kinase (MAPK), and Wnt/β-catenin signaling pathway [37]. In addition, tetrahydroxystilbene glucoside, a unique component of the bone-reinforcing herbal preparation Radix Polygoni Multiflori, was shown to activate the PI3K/Akt pathway, and thus promote MC3T3-E1 cell proliferation and differentiation. Together with the proven influence on the expression of osteoprotegerin (OPG)/nuclear factor-κB ligand (RANKL)/macrophage colony-stimulating factor (M-CSF), this suggests that this tetrahydroxystilbene glucoside might be a potential therapeutic agent for osteoporosis [38].
Antimicrobial and antifungal properties of constituents of medicinal plants have been widely reported [39]. In our issue, Xiao et al. reported that berberine hydrochloride exhibited inhibitory effects on the growth of Trichophyton mentagrophytes, which might be associated with down-regulation of 14α-demethylase [40].
This special issue also includes two reviews. One summarized the available ethnobotanical, phytochemical, and pharmacological information about the traditional Amazonian medicinal tree Maytenus macrocarpa (Celastraceae), which is traditionally used mainly to treat rheumatism and, to a lesser extent, to heal wounds and to combat bronchitis and diarrhea [41]. The other one provided an overview of recent advances in the production of hybrid nanogels containing different biomolecules for various biomedical applications, and commented, among others, on their key physical consequences, functionalization, and advantages [42].
Overall, the Special Issue "Bioactive Molecules and Their Mechanisms of Action" gives a current snapshot on the multifaceted interdisciplinary character of this research topic, as evident in the wide range of addressed questions and employed methodologies. Considering the challenges in the field of drug discovery, this issue complements not only our knowledge on bioactives, but may also provide some novel ideas and inspiration for further investigation of diverse bioactive molecules with the potential for future therapeutic development.

Conflicts of Interest:
The author declares no conflict of interest.