MicroRNA-29a Attenuates Diabetic Glomerular Injury through Modulating Cannabinoid Receptor 1 Signaling
1
Department of Nephrology, Chang Gung Memorial Hospital, Chiayi 61363, Taiwan
2
Kidney and Diabetic Complications Research Team (KDCRT), Chang Gung Memorial Hospital, Chiayi 61363, Taiwan
3
Division of Endocrinology and Metabolism, Chang Gung Memorial Hospital, Chiayi 61363, Taiwan
4
Department of Anatomic Pathology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, College of Medicine, Kaohsiung 83301, Taiwan
5
Kidney Research Center, Chang Gung Memorial Hospital, Taipei 10507, Taiwan
6
College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
7
Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, College of Medicine, Kaohsiung 83301, Taiwan
*
Authors to whom correspondence should be addressed.
†
Both authors contributed equally to this work as co-first authors.
Molecules 2019, 24(2), 264; https://doi.org/10.3390/molecules24020264
Received: 29 October 2018 / Revised: 1 January 2019 / Accepted: 7 January 2019 / Published: 11 January 2019
(This article belongs to the Special Issue Emerging Topics in (Endo)Cannabinoid Signalling)
Diabetic nephropathy often leads to end-stage renal disease and life-threatening morbidities. Simple control of risk factors is insufficient to prevent the progression of diabetic nephropathy, hence the need for discovering new treatments is of paramount importance. Recently, the dysregulation of microRNAs or the cannabinoid signaling pathway has been implicated in the pathogenesis of various renal tubulointerstitial fibrotic damages and thus novel therapeutic targets for chronic kidney diseases have emerged; however, the role of microRNAs or cannabinoid receptors on diabetes-induced glomerular injuries remains to be elucidated. In high-glucose-stressed renal mesangial cells, transfection of a miR-29a precursor sufficiently suppressed the mRNA and protein expressions of cannabinoid type 1 receptor (CB1R). Our data also revealed upregulated CB1R, interleukin-1β, interleukin-6, tumor necrosis factor-α, c-Jun, and type 4 collagen in the glomeruli of streptozotocin (STZ)-induced diabetic mice, whereas the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) was decreased. Importantly, using gain-of-function transgenic mice, we demonstrated that miR-29a acts as a negative regulator of CB1R, blocks the expressions of these proinflammatory and profibrogenic mediators, and attenuates renal hypertrophy. We also showed that overexpression of miR-29a restored PPAR-γ signaling in the renal glomeruli of diabetic animals. Collectively, our findings indicate that the interaction between miR-29a, CB1R, and PPAR-γ may play an important role in protecting diabetic renal glomeruli from fibrotic injuries.
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MDPI and ACS Style
Tung, C.-W.; Ho, C.; Hsu, Y.-C.; Huang, S.-C.; Shih, Y.-H.; Lin, C.-L. MicroRNA-29a Attenuates Diabetic Glomerular Injury through Modulating Cannabinoid Receptor 1 Signaling. Molecules 2019, 24, 264. https://doi.org/10.3390/molecules24020264
AMA Style
Tung C-W, Ho C, Hsu Y-C, Huang S-C, Shih Y-H, Lin C-L. MicroRNA-29a Attenuates Diabetic Glomerular Injury through Modulating Cannabinoid Receptor 1 Signaling. Molecules. 2019; 24(2):264. https://doi.org/10.3390/molecules24020264
Chicago/Turabian StyleTung, Chun-Wu; Ho, Cheng; Hsu, Yung-Chien; Huang, Shun-Chen; Shih, Ya-Hsueh; Lin, Chun-Liang. 2019. "MicroRNA-29a Attenuates Diabetic Glomerular Injury through Modulating Cannabinoid Receptor 1 Signaling" Molecules 24, no. 2: 264. https://doi.org/10.3390/molecules24020264
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