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Open AccessArticle

Formation and Stabilization of Gold Nanoparticles in Bovine Serum Albumin Solution

“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, Splaiul Independentei 202, 060021 Bucharest, Romania
Author to whom correspondence should be addressed.
Academic Editor: Chryssostomos Chatgilialoglu
Molecules 2019, 24(18), 3395;
Received: 22 August 2019 / Revised: 13 September 2019 / Accepted: 17 September 2019 / Published: 18 September 2019
(This article belongs to the Special Issue Biomimetic Radical Chemistry and Applications)
The formation and growth of gold nanoparticles (AuNPs) were investigated in pH 7 buffer solution of bovine serum albumin (BSA) at room temperature. The processes were monitored by UV-Vis, circular dichroism, Raman and electron paramagnetic resonance (EPR) spectroscopies. TEM microscopy and dynamic light scattering (DLS) measurements were used to evidence changes in particle size during nanoparticle formation and growth. The formation of AuNPs at pH 7 in the absence of BSA was not observed, which proves that the albumin is involved in the first step of Au(III) reduction. Changes in the EPR spectral features of two spin probes, CAT16 and DIS3, with affinity for BSA and AuNPs, respectively, allowed us to monitor the particle growth and to demonstrate the protective role of BSA for AuNPs. The size of AuNPs formed in BSA solution increases slowly with time, resulting in nanoparticles of different morphologies, as revealed by TEM. Raman spectra of BSA indicate the interaction of albumin with AuNPs through sulfur-containing amino acid residues. This study shows that albumins act as both reducing agents and protective corona of AuNPs. View Full-Text
Keywords: gold nanoparticles; albumin; EPR spectroscopy; Raman spectroscopy; circular dichroism gold nanoparticles; albumin; EPR spectroscopy; Raman spectroscopy; circular dichroism
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MDPI and ACS Style

Matei, I.; Buta, C.M.; Turcu, I.M.; Culita, D.; Munteanu, C.; Ionita, G. Formation and Stabilization of Gold Nanoparticles in Bovine Serum Albumin Solution. Molecules 2019, 24, 3395.

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