Comparison of the Utility of RP-TLC Technique and Different Computational Methods to Assess the Lipophilicity of Selected Antiparasitic, Antihypertensive, and Anti-inflammatory Drugs

The aim of this study was to assess the lipophilicity of selected antiparasitic, antihypertensive and non-steroidal anti-inflammatory drugs (NSAIDs) by means of reversed phase–thin layer chromatography (RP-TLC) as well by using Soczewiński–Wachtmeister’s and J. Ościk’s equations. The lipophilicity parameters of all examined compounds obtained under various chromatographic systems (i.e., methanol-water and acetone-water, respectively) and those determined on the basis of Soczewiński-Wachtmeister’s and Ościk’s equations (i.e., RMWS and RMWO) were compared with the theoretical ones (e.g., AlogPs, AClogP, milogP, AlogP, MlogP, XlogP2, XlogP3) and the experimental value of the partition coefficient (logPexp). It was found that the RMWS parameter may be a good alternative tool in describing the lipophilic nature of biologically active compounds with a high and low lipophilicity (i.e., antihypertensive and antiparasitic drugs). Meanwhile, the RMWO was more suitable for compounds with a medium lipophilicity (i.e., non-steroidal anti-inflammatory drugs). The chromatographic parameter φ0(a) can be helpful for the prediction of partition coefficients, i.e., AClogP, XlogP3, as well as logPexp of examined compounds.


Introduction
Lipophilicity is an important parameter widely used in medicinal chemistry in the design of new drug candidates.This physicochemical property provides the most important information about a proper biomolecule, including its binding to a suitable receptor and ADMET (Absorption, Distribution, Metabolism, Excretion and Toxicity) properties [1].What is more, in combination with steric and electron factors, it is a powerful tool in the development of novel drugs and their analogues with a greater effectiveness by means of SAR (Structure-Activity Relationship) and QSAR (Quantitative Structure-Activity Relationship) studies, respectively [2][3][4].Different theoretical as well as experimental methods for determining the lipophilicity are distinguished [5,6].Among them, the most frequently used are those based on a simple extraction in the n-octanol-water system such as the classical shake-flask method [5,6].However, due to limitations, this procedure is frequently being replaced by chromatographic techniques.
The current literature review indicates that reversed-phase liquid chromatography (RP-LC) is an alternative method for the determination of the lipophilicity of various biocompounds by modeling the distribution of a proper compound between lipid layers and biological membranes of cells [5].
The first well known kind of liquid chromatography that was widely used to establish the lipophilicity of various chemical compounds was thin-layer chromatography (TLC).This technique performed in reversed phase system (RP-TLC) has many advantages over the shake-flask method.Moreover, a higher reproducibility of the obtained lipophilicity results can be observed [3,5].
In addition to experimental methods, in order to determine the n-octanol-water partition coefficient (logP), different theoretical methods (computer programs) allowing the initial assessment of the logP value can also be applied [3].Many various lipophilicity descriptors and methods have been well described and proposed for the evaluation of the lipophilic properties of different compounds in review articles from recent years [7][8][9].Currently, the chromatographic approach is used the most [10][11][12][13][14][15][16][17][18].
The knowledge concerning the hydrophilic-hydrophobic properties of studied antiparasitic drugs in the current literature is spare and contradictory.For example, Biagi [19] has reported that tididazole and metronidazole are hydrophilic compounds.Másson et al. [20] has shown that metronidazole is a very hydrophilic compound.However, according to Hoffmann et al. [21] and Jokipii et al. [22], metronidazole is a lipophilic compound that penetrates well into most tissues.Next, Guerra et al. [23] studied the relationship between the values of the chromatographic parameter R M of antiparasitic drugs and their logP values.The RP-TLC technique was used to determine the R M values of metronidazole, ornidazole and tinidazole, but no secnidazole was studied in this case.The lipophilicity parameter (R MW ) estimated by Soczewi ński-Wachtmeister's equation was 0.08 for metronidazole, 0.33 for ornidazole, and 0.35 for tinidazole, respectively.It was also noted, that there are significant differences between the theoretically calculated and experimentally determined logP values of nitroimidazoles, similarly like in the study by Lin et al. [24].
Moreover, Guerra et al. [23] studied the R M values of metronidazole, ornidazole, and tinidazole in the chromatographic systems, where the stationary phase was squalene, undecane and liquid paraffin.Additionally, the described R M parameter was measured on unimpregnated layers of silica gel.The lipophilicity parameter (R MW ) obtained by using Soczewi ński-Wachtmeister's equation indicated that the addition of silicone oil to the stationary phase can modify the partitioning of the examined compound between both, i.e., the stationary phase and mobile phase, and thus their chromatographic parameter of lipophilicity.
The lipophilicity of selected antiparasitic drugs was also investigated by Guerra et al. using the RP-HPLC technique [25].The measured logk' value for metronidazole was -0.067, for ornidazole it was 0.199 and for tinidazole it was -0.125, but secnidazole was not studied.The values of this chromatographic parameter correlated better with the logP values determined in the n-octanol-water system than the previously determined R M parameters of the tested compounds [23].
Next, Sârbu et al. [26] investigated the lipophilicity of selected non-steroidal anti-inflammatory drugs (NSAIDs), e.g., indomethacin, ketoprofen and phenylbutazone, by using the RP-TLC technique, but did not study mefenamic acid, nabumetone, carprofen and flurbiprofen.For this purpose, the chromatographic plates precoated with RP-18WF 254 and Nano-Sil CNF 254 were used, and the mobile phase consisted of methanol and water.The lipophilicity parameter (R MO ) obtained by using Soczewi ński-Wachtmeister's equation on RP-18WF 254 plates was 2.62 for indomethacin, 1.74 for ketoprofen and 2.71 for phenylbutazone.Meanwhile, the R MO value estimated on Nano-Sil CNF 254 plates for indomethacin, ketoprofen and phenylbutazone was 0.52, 2.31, as well as 1.50, respectively.Another author, Pehourq et al. [27], studied the lipophilicity expressed as log k of some NSAIDs, e.g., carprofen, flurbiprofen and ketoprofen, using the RP-HPLC technique, but mefenamic acid, nabumetone, indomethacin and phenylbutazone were not investigated.The following stationary phases, such as the ODS column prepacked with µBondapak C18 and an immobilized artificial membrane (IAM.PC.MG) column, were used in this study.The values of both the parameters log k wODS and log k w IAM were 3.85 and 1.81 for carprofen, 2.91 and 1.58 for flurbiprofen, and 2.83 and 1.02 for ketoprofen.Significant linear correlations (r > 0.94) between the chromatographic parameters (logk w IAM ) and the reference lipophilicity data (logP and logD 7.4 ) were obtained.
In a work prepared by Czyrski [28], the lipophilicity among other NSAIDs like ketoprofen and flurbiprofen using RP-18F 254 plates and the mobile phase composed of acetonitrile and water was studied.The R m0 values were determined for the compounds with a known logP and for ketoprofen (1.8491) and flurbiprofen (2.5076), respectively.Next, the lipophilicity parameters (logP) were calculated for the analyzed compounds using the prepared regression curve of type R m0 = f (logP).It was stated that flurbiprofen has the highest logP value, equal to 3.82, while ketoprofen has the lowest one, equal to 2.66.
As of now, there is no study in the scientific literature concerning the lipophilicity assessment of antihypertensive drugs, e.g., nilvadipine, felodipine, isradipine and lacidipine via the use of the TLC and calculation methods.Moreover, the values of the partition coefficient determined experimentally in the n-octanol-water system (logP exp ) (available in the literature as well as online via databases) for drugs analyzed in this work belonging to antiparasitic and antihypertensive agents as well as NSAIDs are very diverse.

Results and Discussion
In present work the lipophilicity of selected members belonging to three different groups of drugs, namely antiparasitic, antihypertensive and NSAIDs, were studied.For all examined compounds, the retention parameters R M were determined by using the RP-TLC technique in various chromatographic systems.The obtained R M values were used to determine two lipophilicity parameters marked as R MWS and R MWO , respectively using Soczewi ński-Wachtmeister's and Ościk's equations.On the basis of the results obtained, the lipophilic properties of the studied drugs were assessed.
The R M values achieved under various chromatographic conditions were extrapolated to the zero content of the organic modifier (ϕ = 0) in the used mobile phase according to the Soczewi ński-Wachtmeister's equation.Next, the equations showing the linear relationship between the R M values and organic modifier content in the used mobile phase were determined, and the R MWS values for particular groups of examined compounds were estimated.Taking into account all cases, a high correlation coefficient r (above 0.95) was observed.All obtained lipophilicity parameters, including chromatographic parameters R MWS and ϕ 0 , as well as R MWO , are presented in Tables 1-3.Where: a R MWS(a) -chromatographic lipophilicity parameter obtained experimentally on the basis of Soczewi ński-Wachtmeister's equation using acetone-water as the mobile phase on silica gel RP-18F 254 ; b R MWS(m) -chromatographic lipophilicity parameter obtained experimentally on the basis of Soczewi ński-Wachtmeister's equation using methanol-water as the mobile phase on silica gel RP-18F 254 ; c R MWO(a) -chromatographic parameter of lipophilicity obtained experimentally on the basis of Ościk's equation using acetone-water as the mobile phase on silica gel RP-18F 254 ; d R MWO(m) -chromatographic parameter of lipophilicity obtained experimentally on the basis of Ościk's equation using methanol-water as the mobile phase on silica gel RP-18F 254 ; e ϕ 0(a) -chromatographic lipophilicity parameter calculated on the basis of the parameters of Soczewi ński-Wachtmeister's equation, i.e., R MWS(a) and S, respectively using Equation ( 6); f ϕ 0(m) -chromatographic lipophilicity parameter calculated on the basis of the parameters of Soczewi ński-Wachtmeister's equation, i.e., R MWS(m) and S, respectively using Equation ( 6).Among the three groups of analyzed compounds, the highest values of the R MWS lipophilicity parameter were obtained for antihypertensive drugs, and they ranged from 3.54 to 4.51 (in the acetone-water system) and from 3.13 to 5.01 (in the methanol-water system).For the examined NSAIDs, the R MWS values range from 1.26 to 3.08 (in the acetone-water system) and from 1.47 to 3.12 (in the methanol-water system).However, the smallest R MWS values were obtained for antiparasitic drugs, and they changed from 0.65 to 1.22 (in the acetone-water mobile phase) and from 0.91 to 1.46 (in the methanol-water system).
The R MWO parameter for the three groups of analyzed compounds in the methanol-water and acetone-water systems on RP-18F 254 plates was carried out in accordance with the methodology presented by Janicka [29,30].The results of this parameter are placed in Tables 1-3.
The data listed in Table 3 indicate that there is a lack of R MWO(a) values for a group of examined (NSAIDs) in the case of the acetone-water mobile phase, because this mixture was not suitable for testing the lipophilicity of these compounds.
A further interpretation of the obtained results shows that among the three groups of analyzed compounds, the highest values of R MWO were obtained for antihypertensive drugs in the methanol-water system as well as the acetone-water mobile phase.The R MWO values are placed in the range of 5.57 to 6.63 (in the acetone-water system) and from 5.08 to 6.89 (for the methanol-water system).However, the smallest R MWO values were observed for antiparasitic drugs.They are placed in the range of 0.94 to 1.42 (in the acetone-water system) and from 1.21 to 1.92 (in the methanol-water system).
The values of both the R MWS and R MWO parameters estimated for all analyzed compounds in the methanol-water and acetone-water systems indicate that the highest lipophilicity is shown by antihypertensive drugs, while the lowest lipophilicity belongs to the antiparasitic drugs.
On the basis of the obtained results, it can be observed that the value of the chromatographic lipophilicity parameter R MWS(a) = 0.65 for metronidazole is similar to R MW(squalene) = 0.53 and R MW(paraffin) = 0.60, which were given in the literature [23].Meanwhile the value of the chromatographic lipophilicity parameter R MWS(a) = 1.22 for ornidazole is comparable to the literature values, which were R MW (undecane) = 0.98, R MW (squalene) = 0.93 and R MW (paraffin) = 1.12, respectively.
All parameters characterizing the lipophilic properties of tinidazole obtained in the present work by Soczewi ński-Wachtmeister's and Ościk's methods have similar values to R MW(undecane) , R MW(squalene) and R MW(paraffin) [23], except for the R MWO(m) value.
The lipophilicity parameters of metronidazole, ornidazole and tinidazole, estimated on the basis of Soczewi ński-Wachtmeister's and Ościk's equations, using the RP-TLC technique (i.e., R MWS and R MWO ), are relatively higher than the results of the appropriate logk' determined by Guerra et al. [25].
In the case of the studied antiparasitic drugs, the chromatographic parameters of these drugs and those available in the literature [23,25] differ from each other.The main reason for this fact can be, for example, the different adsorbing properties of applied stationary phases or eluents such as their elution strength or surface tension, which show an influence on the results of the R M parameter.As was confirmed in previous studies, the pH value of distilled water used as a mobile phase component also has a large impact on the value of both the chromatographic lipophilicity parameters and the experimental partition coefficients [8,11,18,31].
The lipophilicity parameters determined on the basis of Soczewi ński-Wachtmeister's equation in the acetone-water and methanol-water systems (i.e., R MWS(a), R MWS(m) ) have values equal to 2.57 and 2.19 for indomethacin and 1.26 and 1.47 for ketoprofen, and they are close to the appropriate R MO values determined on RP-18WF 254 plates by Sârbu et al. [26].
All parameters describing the lipophilic properties of carprofen, flurbiprofen and ketoprofen estimated in this work via Soczewi ński-Wachtmeister's and Ościk's methods have lower values in relation to the values of the appropriate log k wODS reported by Pehourq et al. [27], except for ketoprofen, for which the R MWO(m) value equal to 3.76 is higher than the literature value of log k wODS .However, the literature values of logk w IAM [27] have a lower value for carprofen, flurbiprofen and ketoprofen than the lipophilicity parameters of R MWS and R MWO determined in the current work via Soczewi ński-Wachtmeister's and Ościk's methods [3,32].
The parameters characterizing the lipophilic properties of flurbiprofen and ketoprofen determined by Soczewi ński-Wachtmeister's and Ościk's methods in this paper have lower values in relation to the values of logP reported by Czyrski [28].The exception is ketoprofen, whose R MWO(m) value is 3.76, which is higher than the literature values of logP.
Because the pH value of distilled water used to determine the partition coefficient in an n-octanol-water system may cause the differences in logP exp values for the abovementioned compounds, the partition coefficient of the synthesized co-prodrug of flurbiprofen and methocarbamol was determined in the three mobile phases, namely: water, n-octanol-water (pH = 1.2) and n-octanol-phosphate buffer (pH = 7.4).Under these conditions the logP o/w values equal to 5.13; 1.32 and 4.72 were observed [31].
Next, a cluster analysis by using the Statistica13.1 program was performed for three groups of analyzed drugs.This analysis precisely groups the most closely related parameters to be compared, like, for example, the described lipophilicity.For the purpose of the cluster analysis, all theoretical partition coefficients and chromatographic parameters, i.e., R MWS and R MWO , except ϕ 0 , were used.
Additionally, the calculated parameter ϕ 0 cannot be compared with the logP values.It may be used for the purpose of a relative comparison of the lipophilic properties of the studied compounds only, and it is helpful in finding the order of lipophilicity degree of the examined group of compounds (from the most lipophilic to the least) [3].The average values of logP exp available for metronidazole, ornidazole, tinidazole, felodipine, indomethacin, ketoprofen, and flurbiprofen, and the values of logP exp for other drugs (i.e., secnidazole, isradipine, mefenamic acid, nabumetone, and phenylbutazone), were also applied for this study.The results of the cluster analysis are presented as dendrograms in Figures 1-3, respectively.Figure 1 presents a comparison of the chromatographically determined lipophilicity parameters with the theoretical ones (Figure 1a) and logP exp (Figure 1b) for the studied antiparasitic drugs.Figure 1 suggests that the parameters describing the lipophilicity of the studied antiparasitic drugs can be divided into two main subgroups.The first includes AlogPs, AClogP, XlogP3, milogP, AlogP, XlogP2 (Figure 1a) or logP exp , AlogPs, AC logP, XlogP3, milogP, AlogP, and XlogP2 (Figure 1b).The second group consists of MlogP and the chromatographic parameters of lipophilicity R MW , determined by Soczewi ński-Wachtmeister's and Ościk's equations.Additionally, this diagrams shows that AClogP and AlogPs have the most similar values among the theoretical parameters.Meanwhile, the theoretical parameter MlogP shows the biggest similarity to the chromatographically determined lipophilicity parameter (R MWS(a) ).The biggest similarity can also be observed between R MWO(a) and R MWS(m) in a group of chromatographically determined lipophilicity parameters.A significant difference in relation to other parameters is indicated by R MWO(m) , determined by the Ościk's equation (Figure 1a,b) and logP exp , which separately forms a single subgroup (Figure 1b).Next, Figure 2 represents a comparison of the chromatographically determined lipophilicity parameters with the theoretical ones (Figure 2a), as well as the logP exp (Figure 2b) obtained for the antihypertensive drugs (except for nilvadipine and lacidipine), for which the logP exp was not given in the literature.
Figure 2a shows that among the theoretical parameters, the biggest similarity indicates XlogP2 and XlogP3.Meanwhile, the theoretical parameter AlogPs is similar to the chromatographically determined lipophilicity parameter (R MWS(m) ). Figure 2b   Next, Figure 3 shows the relation observed between the chromatographically determined lipophilicity parameters and the theoretical ones (Figure 3a) and the logP exp , respectively (Figure 3b) for the studied NSAIDs (except of carprofen, for which the logP exp was not available).
Figure 3a,b demonstrate that the biggest similarity is indicated by the chromatographically determined lipophilicity parameters R MWS(a) and R MWS(m) .The values of R MWO(m) obtained by Ościk's equation show the biggest connection to the theoretical parameters such as milogP and MlogP, due to the Euclidean distance.However, the logP exp (Figure 3b) shows the biggest similarity to the XlogP3 and AlogPs, due to the Euclidean distances.
Taking into account a strong connection between the chromatographically determined lipophilicity parameters R MWS and R MWO , respectively, as well as the theoretical and experimental parameters (logP) obtained for the three groups of analyzed drugs, it can be concluded that the R MWS value may be a good alternative tool in describing the lipophilic nature of biologically active compounds having a high as well as low lipophilicity (e.g., antihypertensive drugs and antiparasitic drugs), respectively.On the other hand, R MWO was found to be more suitable for the compounds with medium lipophilicity (i.e., NSAIDs).
The continuation of our lipophilicity study of fifteen biocompounds belonging to the three groups of drugs and the determination of another lipophilicity descriptor, namely ϕ 0 , in accordance with Equation ( 6), allowed us to obtain the two new lipophilicity parameters ϕ 0(a) and ϕ 0(m) for a proper used mobile phase, i.e., acetone-water (a) and methanol-water (m), respectively.What is important is that the calculation of this parameter was possible because a satisfactory linear relationship between both, i.e., the intercept and slope (R MWS and S), in each obtained Soczewi ński-Wachtmeister's equation was achieved (r > 0.98).In order to estimate the utility of these parameters for the prediction of the theoretical as well as experimental parameter of the lipophilicity of a large group of investigated compounds, a correlation matrix between all previously described (thus chromatographic) parameters, as well as the computed and experimental partition coefficient, was done.The results of the correlation analysis indicate that among all ϕ 0 values the best is the one obtained for acetone-water, which is to say ϕ 0(a).The most satisfactory linear correlations (r > 0.89) were obtained between the following partition coefficients: AClogP, XlogP3, logP exp and the ϕ 0(a) values (see Equations ( 1)-( 3 This fact confirms the potential utility of the additionally calculated chromatographic parameter ϕ 0(a) to determine the partition coefficient of all examined compounds.It can be very useful in the case of the lack of this value, like for example for the experimental logP of nilvadipine, lacidipine and carprofen.A test was performed on Equation (3) to determine the values of logP exp for the three abovementioned compounds.The results of the predicted logP exp were: 3.65 for nilvadipine, 3.96 for lacidipine and 3.54 for carprofen, respectively.It can be observed that the newly obtained logP exp values are in good agreement with the other previously described theoretical logP of these compounds.They are placed in the similar range of logP values.
Summing up, the applied RP-TLC method and lipophilicity parameters denoted by R MWS and R MWO and calculated by using the retention parameter (R M ) in accordance with Soczewi ński-Wachtmeister's and Ościk's equations, respectively, may be the alternates to other lipophilicity descriptors (such as for example the logP determined by the classical shake-flask method) in describing the lipophilic character of bioactive compounds belonging to the following groups of drugs: antiparasitic, antihypertensive and non-steroidal anti-inflammatory drugs.

Chemicals and Standard Solutions
The standard solutions of all investigated compounds: metronidazole, ornidazole, secnidazole, tinidazole belonging to antiparasitic drugs; nilvadipine, felodipine, isradipine, lacidipine, used as antihypertensive agents; and a few non-steroidal anti-inflammatory drugs: mefenamic acid, indomethacin, nabumetone, phenylbutazone, carprofen, ketoprofen, and flurbiprofen, were supplied by Sigma-Aldrich (St. Louis, MO, USA).The solvents methanol and acetone, which have been used as mobile phase components, were from Merck (Darmstadt, Germany).The distillated water was from the Department of Analytical Chemistry (School of Pharmacy and the Division of Laboratory Medicine, Medical University of Silesia, Sosnowiec, Poland).Standard solutions of antiparasitic drugs and antihypertensive drugs at concentrations of 10 mg/mL each were prepared in methanol (Merck, Darmstadt, Germany).The solutions of indomethacin, nabumetone, phenylbutazone and ketoprofen at concentrations of 1 mg/mL each were prepared in acetone (Merck, Darmstadt, Germany).Standard solutions of carprofen and flurbiprofen at concentrations of 1 mg/mL each were prepared in ethanol (99.8%,POCh, Gliwice, Poland).A solution of mefenamic acid at a concentration of 1 mg/mL was prepared in a chloroform-methanol mixture in a volume ratio of 3:1.Chloroform was procured from POCh (Gliwice, Poland).All reagents had an analytical grade of purity.

RP-TLC
The chromatographic analysis was carried on RP-18F 254 plates (Art.1.05559,E. Merck, Darmstadt, Germany).The solutions of the investigated compounds were spotted separately onto chromatographic plates using precise micropipettes in a quantity of 5 µL each.
The chromatograms were developed using the mixtures of methanol-water and acetone-water in different volume compositions, as follows: For antiparasitic drugs: the content of methanol was gradually varied by 10% (v/v) from 20-100 (%, v/v), -the content of acetone was gradually varied by 10% (v/v) from 10-100 (%, v/v).
In the case of antihypertensive drugs, the content of methanol and acetone was gradually varied by 5% (v/v) from 60-100 (%, v/v).
Fifty mL of used mobile phase was placed into a classical chromatographic chamber (Art.022.5255,Camag, Muttenz, Switzerland).Next, the chamber was saturated with solvent vapor for 20 min.The chromatograms were developed at room temperature, e.g., 22 ± 1 • C. The development distance was 75 mm.The plates were dried at room temperature, e.g., 22 ± 1 • C. Each chromatogram was done in triplicate.

Densitometric Analysis
Densitometric scanning was done using a TLC Scanner 3 with WinCATS 1.4.2software manufactured by Camag (Muttenz, Switzerland) in the reflectance/absorbance mode.
Densitometric scanning was performed at a respective absorption maximum for the analyzed drugs.The slit dimensions were 10.00 × 0.40 mm, Macro; the optimized optical system was light; the scanning speed was 20 mm/s; the data resolution was 100 µm/step; the measurement type was remission; the measurement mode was absorption; and the optical filter was second order.Each track was scanned three times, and a baseline correction (lowest slope) was used.
The linear relationship between R M and the volume content of methanol and acetone in the mobile phase (ϕ) permits an extrapolation of the calculated R M values to the zero concentration of methanol and acetone in accordance with Soczewi ński-Wachtmeister s Equation (5).The value of the intercept (R MWS ) represents the lipophilicity parameter of the examined compound [2,3].
where: R M is the R M value of the studied compound, R MWS is the R M value extrapolated to zero concentration of methanol and acetone (organic modifier) in the used mobile phase, i.e., methanol-water and acetone-water, respectively, S is the slope of the regression plot (see Supplementary Materials), and ϕ is the volume fraction of methanol and acetone in the mobile phase.In addition to this, on the basis of the said plot, i.e., Soczewi ński-Wachtmeister s equation, another chromatographic descriptor (ϕ 0 ) has been calculated according to the following formula (Equation ( 6)): where: R MWS is the chromatographic parameter obtained by using Soczewi ński-Wachtmeister's equation in accordance with Equation ( 5), and S is the regression slope.

Chromatographic Parameter of Lipophilicity R MWO
The measurable lipophilicity value expressed as R MWO was determined according to Ościk's equation [29,30,32]: where: R M , R Morg , and R MWO are the solute retention factors in the mixed mobile phase, pure organic solvent, and water, respectively; x org is the molar fraction of the organic solvent in the mobile phase; a and b are constants in the linear correlation between G(x org ) and x org in the used mobile phase.

Determining the Theoretical and Experimental Partition Coefficients (logP)
The values of the theoretical partition coefficients, such as AlogPs, AClogP, AlogP, MlogP, XlogP2, and XlogP3, for the examined compounds, were obtained from the internet database VCCLAB [33].The theoretical partition coefficient, milogP, for the examined compounds was from another internet database, namely Molinspiration Cheminformatics [45].Therefore, different algorithms based on the chemical structure of all the tested compounds were applied for the prediction of the logP value [36,46].

Figure 1 .
Figure 1.Similarity analysis for antiparasitic drugs, including: (a) comparison of the chromatographic and theoretical parameters of lipophilicity.(b) comparison of the chromatographic, theoretical and experimental parameters of lipophilicity.

Figure 2 .
Figure 2. A similarity analysis for antihypertensive drugs, including: (a) comparison of the chromatographic and theoretical parameters of lipophilicity.(b) comparison of the chromatographic, theoretical and experimental parameters of lipophilicity.
suggests that the theoretical parameter XlogP2 indicates the biggest similarity to the logP exp value.However, the AlogPs and AClogP values show the biggest connection to the chromatographically determined lipophilicity parameters (R MWS(m) and R MWS(a) ), respectively.The R MWO(m) and R MWO(a) determined by the Ościk's equation form a separate subgroup (Figure 2a,b).

3. 4 .
Calculations 3.4.1.Chromatographic Parameters of Lipophilicity R MWS and ϕ 0 In order to determine the lipophilicity parameter based on Soczewi ński-Wachtmeister s procedure, the R F values obtained under the applied chromatographic conditions were converted into R M values according to the expression: R M = log(1/R F − 1)

Table 1 .
Summary of the lipophilicity study of antiparasitic drugs by using the theoretical and RP-TLC methods.

Table 2 .
Summary of the lipophilicity study of antihypertensive drugs by using the theoretical and RP-TLC methods.

Table 3 .
Summary of the lipophilicity study of NSAIDs by using the theoretical and RP-TLC methods.

Table 4 .
The experimental partition coefficients (logP exp ) of the examined compounds.