The Influence of Yeast Strain, β-Cyclodextrin, and Storage Time on Concentrations of Phytochemical Components, Sensory Attributes, and Antioxidative Activity of Novel Red Apple Ciders

The yeast strain and storage time is an important factor affecting the development of phytochemicals and sensory attributes in ciders. Therefore, the aim of this study was to determine the influence of yeast strains (Saccharomyces bayanus and Saccharomyces cerevisiae), β-cyclodextrin (BCD), and storage time on physicochemical parameters, contents of phenolic compounds (ultra-performance liquid chromatography with photodiode array detector coupled to quadrupole time-of-flight tandem mass spectrometry (UPLC–PDA–QToF-MS/MS)), antioxidative activity (free radical-scavenging ability (ABTS) and ferric reducing antioxidative power (FRAP) assay), and sensory attributes of new cider from the “Bella Marii” cultivar of red apple. The pH value, acidity, concentrations of alcohol, organic acids, and polyphenols; and the color and antioxidative properties were evaluated in red apple ciders immediately after fermentation and after three months of storage at 4 °C. S. cerevisiae SIHAFERM Finesse Red with BCD and SIHAFERM Finesse Red yeast strain especially contributed to obtaining ciders with a high content of the tested compounds. The use of BCD during fermentation significantly influenced the protection of bioactive compounds, by as much as 18%. Storage time had an impact on concentrations of the tested components (mainly on the total flavan-3-ols and phenolic acids). Based on the achieved values of parameters analyzed in red apple ciders and results of the consumer acceptance test, it may be concluded that red apple offers vast potential for the production of ciders with a high content of polyphenolic compounds.


Introduction
Cider is a fermented beverage with alcohol content from 1.2 to 8.5% v/v made mainly of apples. It should be emphasized that its quality and character are determined foremost by the apples used in its production process. Special attention should be paid to the contents of polyphenols, acids, tannins, and sugars in the apples, because they are responsible for the sensory characteristics of this fermented beverage [1]. The polyphenolic compounds, including anthocyanins, contribute to the development of mainly color, bitterness, and astringency. They may also influence the sweetness and sourness of food products, especially of alcohol products [2,3]. Moreover, they can influence the Table 1. The alcohol, total acidity, pH values, and color parameters in red apple ciders. L*-lightness; a*-redness; b*-yellowness; ∆E-total color difference; ∆C-chroma difference; BCD-β-cyclodextrin.

Storage
Type The pH value and the total acidity of red apple ciders before and after storage at 4 • C were not significantly affected by the yeast strain added. Their mean pH value was 3.28 and their total acidity was 6.37 g/L. The storage time had a significant effect on the total acidity, which ranged from 6.25 g/L before storage to 6.51 g/L after storage (p < 0.05), and these results were similar to the acidity determined in ciders from green apple accounting for 6.70 g/L on average [13]. The mean content of total organic acids determined in red apple cider was not significantly (p < 0.05) affected by the yeast strain used, and it reached 7865.52 mg/L ( Table 2). In contrast, storage time had a significant effect on the content of both total organic acids and individual acid (p < 0.05). Among the eight organic acids analyzed in red apple cider, the main ones were malic acid (69.5%) > citric acid (16.6%) > oxalic acid (4.2%) > quinic acid (4.1%) > succinic acid (<2.8%) ≥ maleic, fumaric, and acetic acid (<0.2%). The major acid in red apple cider before and after storage turned out to be malic acid, which represented around 90% of the total acidity [13,14]. Its content remained stable regardless of yeast addition and storage time and was on average 4.4 times higher than in green apple fruit [15]. In turn, the addition of BCD with S. cerevisiae SIHAFERM Finesse Red to the red apple cider caused an increase by 1.3 and 1.8% and a decrease by 1.3% in the contents of oxalic, fumaric, and citric acids, respectively. The organic acids identified in ciders may originate directly from the raw material or may be formed during fermentation. The first group of acids includes malic, citric, oxalic, quinic, maleic, and fumaric acids, contents of which depend on the cultivar, climate, region, and degree of apple ripeness. The acids appearing after fermentation include succinic, acetic, and lactic acid. The total content of organic acids in cider determines their acidity, which is an important trait responsible for cider color and for the course of the fermentation process. Furthermore, the total acidity and the content of acids have a direct impact on the flavor of ciders [13,16,17]. In turn, the low pH values and high contents of organic acids impact their microbiological stability [17]. This, it is important to test and check the impact of yeast strain and storage time on the values of these parameters in ciders. Moreover, these parameters improve the proper function of the body through regulate the proper chemical reactions and the secretion of digestive enzymes [17]. Color was one of the main quality attributes tested in red apple ciders after fermentation and after storage at 4 • C (Table 1). Generally, the appearance of beverages and food products affects their choice by consumers, whose decisions are driven by such criteria as overall acceptability, sensory characteristics, and safety. The main problem of apple ciders is their browning [17,18]. The red apple ciders were evaluated, before and after storage, for color parameters lightness (L*), yellowness (b*), and redness (a*), and also for chroma difference (∆C), and total color difference (∆E) ( Table 1). Values of color components determined for the red apple juice were L* = 65, a* = 45, and b* = 53. In the case of ciders, color parameters differed significantly depending on the type of yeast used, storage time, and BCD addition (p < 0.05). Statistically significant differences were determined for values of the L* parameter, corresponding to brightness, in all ciders. The use of yeast S. bayanus during fermentation contributed to on average 1.3 times higher brightness of the samples compared to the use of S. cerevisiae. In turn, the red apple cider was 1.2 times brighter than the samples before storage. The lowest value of the L* parameter was recorded in red apple cider fermented with S. cerevisiae (SIHAFERM Finesse Red with BCD and Rubino Cru). The use of BCD resulted in the darkening of the samples by 1.6 and 1.9 times on average before and after storage, respectively. The value of color parameter a* increased, whereas that of parameter b* decreased in all samples of red apple cider after fermentation and maturation. It was indicated by a less yellow and more red color of the final product. The yeast strains, storage time, and BCD addition influenced the L*, a*, and b* values to various extents. The red apple cider fermented with S. cerevisiae (SIHAFERM Finesse Red with BCD and SIHAFERM Finesse Red) was characterized by the highest value of parameter a*, which was indicated by its more intense red color. The use of these yeasts with or without BCD improved cider color during fermentation as well as after treatment. These ciders were characterized by an intense red and attractive color compared to these made with the other yeast tested. The ∆E parameter allows distinguishing colors of two products when ∆E ≥ 5 units [19]. It is a valid parameter for the industry, and, in the analyzed red apple ciders, its values depended on storage time and yeast strain. Its highest value (∆E > 5 units) was determined for the cider fermented with S. cerevisiae (especially SIHAFERM Finesse Red with BCD). The ∆C parameter defines the chromaticity, being a measure of color intensity or saturation, and its values range from 0 (completely unsaturated) to 100 or more (pure color) [20]. In the present study, ∆C significantly depended on yeast strain and storage time, and its highest value was found for the ciders fermented with S. bayanus (25.49), and the lowest one for the sample with the addition of BCD (9.73). In red apple ciders, the ∆C average value was 1.4 times higher than in the ciders before storage.
The results of the fermentation kinetics analysis are illustrated in Figure 1. It was found that the fermentation of SIHAFERM Finesse Red with the addition of BCD was the fastest, with a weight loss of 24.54 g after 24 h. However, the remaining yeast cultures started fermentation later and its kinetics was slower values. The kinetics of fermentation depends on such factors as acidity, temperature, sugar content in the raw material, and the concentration of ethyl alcohol increasing during the process [21,22]. The present research shows fermentation kinetics to range from 5.21 to 5.44 g/100 cm 3 . In contrast, in the study of [19], the kinetics of apple musts Satora fermentation with monocultures and mixed cultures was from 9.14 to 10.07 g/100 cm 3 [23]. This difference results from the use of other apple cultivars characterized by a different acidity and total sugar content. However, studies by Boudreau et al. [24] showed that commonly used fungicides remained on apples and contributed to the reduction of fermentation kinetics during cider production. What is more, the kinetics of fermentation depends on the yeast strain used [1]. According to literature data, yeast strains of S. bayanus are characterized by lower fermentation kinetics than strains of yeast S. cerevisiae [23]. This may be due to the low viability or activity of the yeast strain used, too high a concentration of sulfur dioxide, or the fact that S. bayanus strains carry out the fermentation process more effectively at low temperatures [25]. In addition, the faster fermentation of the cider with SIHAFERM Finesse Red and BCD may be due to the presence of BCD. According to Białecka-Florjanczyk and Majewska [26], BCD may interact with the substrate and reduce its concentration, affecting the course of the fermentation process, and also the type of changes occurring during fermentation [10].

Determination of Polyphenolic Compounds in Red Apple Cider
Phenolic compounds are very important group of bioactive compounds, because they have a strong influence on quality attributes of food products such as taste, aroma, and appearance as well as exhibit health-promoting properties (anti-diabetic, anti-inflammatory, antitumor, anti-allergic, antioxidative) [27][28][29][30][31][32]. Moreover, the cider-making process, yeast strain, and storage time are known to decrease polyphenols concentration in ciders. Furthermore, these factors may affect cider quality. Therefore, it is important to test the effect of the strains of yeast or storage time on the content of polyphenolic bonds and their protection. The identification of polyphenolic compounds extracted from red apple ciders was based on results of the ultra-performance liquid chromatography with photodiode array detector coupled to quadrupole time-of-flight tandem mass spectrometry (UPLC-QToF-MS/MS) analysis, retention time (Rt), ultraviolet-visible light (UV-vis) spectra, and data comparisons [27][28][29][30][31]. In the red apple ciders studied, 33 polyphenolic compounds were separated, belonging to the five groups that are presented in Tables 3 and 4. The major group of polyphenols in red apple ciders were phenolic acids, including mainly hydroxycinnamic acid derivatives which accounted for 39% of all compounds. The next four different fractions of polyphenols included flavan-3-ols (36%; in this group, polymeric proanthocyanidin accounted for 24%) > anthocyanins (13%) > dihydrochalcones (9%) > flavonols (<3%). These compounds are important in ciders and/or wines because they affect their color, taste, aroma, and stability, in addition to their beneficial human effects [32]. Yeast strain and storage time had a significant (p < 0.05) effect on the concentration of polyphenols. The red apple ciders fermented with S. cerevisiae with the addition BCD were rich in the analyzed compounds whose concentrations were 1.3 and 1.2 times higher on average compared to the ciders prepared with S. cerevisiae and S. bayanus. BCD affected the stability and increased the protection of phenolic compounds, which was confirmed in stored chokeberry juice by Lachowicz et al. [9]. The lowest concentration of polyphenolic compounds was determined in the red apple cider prepared with the addition of S. bayanus (412.22 mg/L). The total content of phenolics decreased by around 8% after three months of storage at 4 • C, and, in the final cider, it ranged between 381.83 and 562.57 mg/L. The content of phenolic compounds analyzed by Alberti et al. [13] and Verdu et al. [28] in cider prepared from green apple was 1.5 and 2.0 times lower than in the ciders prepared from red apple. The red apple cider having the highest concentration of these compounds was prepared with the addition of S. cerevisiae SIHAFERM Finesse Red with BCD. In general, stabilization and protection of polyphenolic compounds, especially anthocyanins, in colored juices, like for example chokeberry juice, is achieved by the clarification with the addition of BCD because they prevent the degradation of these compounds. The protection of polyphenolic compounds is very important in the final product due to their health benefits action, therefore BCD with SIHAFERM Finesse Red yeast was used to assure the greater protection of polyphenols [9,33].

Phenolic Acids
The first and major group of phenolic compounds in red apple ciders after fermentation by S. cerevisiae and S. bayanus and storage was phenolic acids. A total of eleven phenolic acids belonging to hydroxycinnamic acids were determined, and they were derivatives of caffeic and p-coumaric acids. The major components of this fraction were represented by three caffeoylquinic acid derivatives identified . These compounds were identified using commercial standards, MS spectra, and also literature data [27][28][29]31] in green apple fruits and ciders. Significant differences (p > 0.05) were found in the contents of phenolic acids, affected by yeast strain and storage time. After storage, an increase by around 3% was determined in phenolic acid content in the red apple cider fermented with S. cerevisiae with the addition of BCD, while a decrease by 6% on average was noted in the other products. After aging, phenolic acid content in the red apple ciders ranged between 137.66 and 180.06 mg/L. According to Alberti et al. [13], hydroxycinnamic acid content determined in juice and cider obtained from green apples was 1.2 and 1.3 times lower compared to the ciders obtained from red apples. The red apple ciders fermented with S. cerevisiae SIHAFERM Finesse Red with the addition of BCD were characterized by the highest content of phenolic acids. In addition, the main compound in the analyzed apple ciders was chlorogenic acid, which accounted for 76% of total phenolic acids. This acid is mainly responsible for the taste values of alcoholic beverages, as well as raw material, and they exhibits antioxidative, antimutagenic, and anticancer properties. Generally, phenolic acids play an important role in the development of astringency and bitterness in wines and/or ciders [9,34]; they are also responsible for the browning of wines or beverages under the influence of oxygen. The use of S. cerevisiae SIHAFERM Finesse Red yeasts with or without BCD influenced the protection of phenolic acids.

Flavan-3-ols
The second fraction of red apple ciders was flavan-3-ols (monomers, oligomers, and polymeric procyanidins) detected as six components as (−)-epicatechin and (+)-catechin with [M − H] − at m/z = 289. Moreover, the red apple ciders contained three B-type procyanidin dimers with m/z = 577 and one trimer with m/z = 865, which were identified using commercial standards and literature [27][28][29]31] in apple fruits and ciders. The yeast strain used in the fermentation process had a significant (p < 0.05) impact on flavan-3-ols content. The red apple ciders obtained with S. cerevisiae with the addition of BCD had a concentration of flavan-3-ols around 1.3 and 1.5 times higher compared to the ciders fermented with the addition of S. cerevisiae and S. bayanus, respectively. Significant differences were noted after storage and with the use of different yeast strains, especially S. cerevisiae with BCD, which caused a 5% increase in flavan-3-ols concentration in the ciders (p < 0.05), while the other yeast strains reduced their concentration after storage by 11% on average. These results confirm the protective effect of the BCD additive on the flavan-3-ol content. Flavan-3-ols are important components of wines and/or ciders. They are responsible for aroma loss, color of the finished product, and also oxidative browning. Flavan-3-ols, especially polymeric procyanidins, directly affect the taste of wines and ciders and contribute to bitterness and astringency development in products [34,35]. Moreover, polymeric procyanidins may undergo polymerization and oxidation, which may cause the browning of wines and ciders [36].

Anthocyanins
The third fraction of the prepared red apple ciders turned out to be anthocyanins. They are responsible for the red to purple color of apple skin and products, and they also exhibit significant antioxidative properties. All seven identified anthocyanins were isomers of cyanidins. These compounds found in rose ciders or red wines often change from monomeric to polymeric, and these changes occur during vinification operations such as fermentation, storage, bottling, and aging.  [28,32,37]. Significant (p > 0.05) differences were noted in the concentration of anthocyanins depending on yeast strain. The use of BCD increased their concentration in the ciders after storage 1.4 and 1.5 times compared to the products fermented with S. cerevisiae and S. bayanus. Similar results for the protection of anthocyanin compounds were obtained for chokeberry juice after clarification with BCD [9]. After storage, a~16% increase was noted in anthocyanin concentration in the cider fermented with S. cerevisiae with the addition of BCD, while a little decrease in these compounds content was determined in other products. After aging time, anthocyanins concentration in the ciders ranged from 48.00 to 91.70 mg/L. The red apple ciders fermented with S. cerevisiae SIHAFERM Finesse Red with the addition of BCD had the highest content of color compounds. The results obtained confirm the protective effect of these yeasts with or without addition of BCDs on anthocyanins. In this group, the predominant anthocyanin was cyanidin-3-O-galactoside, which accounted for 70% of all these components, which is in agreement with findings reported by Knebel et al. [38] for red apple juice. Furthermore, red-colored proanthocyanins are formed during the processing and maturation of fruit juices [38]. In turn, carboxypyranoanthocyanin is formed under the influence of a cycloaddition of pyruvic acid in the enol form and cyanidin-3-O-galactoside. Then, the color of this compound is more resistant to pH changes. Moreover, changes that take place during ciders production and storage time result in browning, which not only negatively affects sensory properties of the product but also decreases its antioxidative capacity [38,39]. Therefore, it is important to choose the right yeast for the production of red apple cider and to control the changes during storage which could affect these compounds.

Dihydrochalcones
Another group of identified phenolic compounds was dihydrochalcones, determined as four compounds with two derivatives of phloretin (with a fragment at m/z = 289) and two derivatives of In addition, two derivatives of phloretin were identified as hydroxyphloretin diglycoside with m/z = 583 and hydroxyphloretin monoglycoside with m/z = 451. These compounds are typical of apples and their products [9,[27][28][29]. There was no significant (p > 0.05) effect of yeast strain and aging time on the content of this fraction of phenolics. After storage, dihydrochalcone concentration in red apple ciders ranged from 35.87 to 45.88 mg/L. The ciders fermented with Lalvin C (S. bayanus) had the lowest content of dihydrochalcones. In addition, the major compound in this group was phlorizin, which accounted for 45% of all dihydrochalcones, and this was in agreement with findings reported by other authors [10,28] for green apples. In addition, Alberti et al. [13] reported that the content of the analyzed compounds determined in juice and cider obtained from green apples was 2.1 and 2.4 times lower compared to the ciders produced from red apples. Dihydrochalcone, mainly phloretin, enhances the action of active components on the surface of lipids, and exhibits strong antioxidative properties [13].

Flavonols
The last fraction of phenolics was represented by five quercetins and their derivatives (with a fragment at m/z = 301) belonging to flavonols. The moieties were determined by classifying them as glucoside (loss of 162 Da from the molecular ion), rhamnoside (loss of 146 Da from the molecular ion), and pentoside (loss of 162 Da from the molecular ion). They were all determined by other authors in green apples [31]. The tested variants of ciders differed significantly (p > 0.05) in this respect depending on yeast strain used and BCD addition. However, yeast strains, especially S. cerevisiae with BCD, caused a 1.4-and 1.6-fold increase of flavonols content in ciders (p < 0.05) compared to the products fermented using S. cerevisiae and S. bayanus alone, respectively. In addition, Alberti et al. [13] reported that the flavonol content determined in green apple juice and cider was 1.7 times higher than in the ciders obtained from red apples. These compounds were the least abundant fraction of the compounds analyzed in red apple ciders; nevertheless, they can exhibit influence the final sensory quality of ciders because they are characterized by low thresholds of astringency. Moreover, flavonols, especially quercetin derivatives, can present anti-inflammatory, anti-allergic, anticoagulant, antitumor, and antiviral properties [31,40].

Determination of Antioxidative Capacity in Red Apple Cider
The antioxidative capacity of the red apple ciders was evaluated based on free radical-scavenging ability (ABTS) and ferric reducing antioxidative power (FRAP) assays ( Table 5). Analyses of all samples before and after storage provided the same trends. No significant (p > 0.05) differences were found between the analyzed variants of samples as affected by yeast strain, storage time, and BCD addition. The antioxidative potential of red apple ciders assessed with the ABTS method ranged from 2.20 and 2.50 mmol Trolox equivalent (TE)/L after fermentation, and from 2.10 to 2.50 mmol TE/L after storage at 4 • C. The antioxidative potential of red apple ciders after aging at 4 • C ranged from 2.30 to 2.60 mmol TE/L (FRAP method). The antioxidative activity tested in green apple juice and ciders with the FRAP method was 2.3 and 2.5 times lower than the average FRAP value measured in red apple ciders [13]. In addition, the major fraction of phenolic compounds presented the highest significant correlation with the antioxidative potential of the analyzed ciders (ABTS, r 2 = 0.96; FRAP, r 2 = 0.83; p < 0.05), which was probably due to the presence of procyanidin polymers, which are known for their high antioxidative potency. Moreover, contents of anthocyanins, phenolic acids, and organic acids were positively correlated with the antioxidative capacity of red apple ciders (ABTS, r 2 = 0.64; FRAP, r 2 = 0.55; ABTS, r 2 = 0.49; FRAP, r 2 = 0.34; ABTS, r 2 = 62; FRAP, r 2 = 0.49; p < 0.05). The high correlation between the biologically active compounds and antioxidative capacity was confirmed in studies of juices and ciders from green apples carried out by Alberti et al. [13] and Laaksonen et al. [31]. Table 5. The antioxidative capacity in red apple ciders before and after storage. TE-Trolox equivalent; ABTS-free radical-scavenging ability; FRAP-ferric reducing antioxidative power.

Storage
Type of Yeast Yeast Antioxidative Activity (mmol TE/L)

Before storage
Saccharomyces bayanus 1 a-e: means ± SD followed by different letters within the same line represent significant differences (p < 0.05). Data are the averages of triplicates. 2 Values are means ± standard deviation; n = 3.

Consumer Evaluation of Red Apple Ciders
The outcome of sensory evaluation of red apple ciders considering their taste, aroma, color, and consistency is illustrated in Figure 2. Generally, the observation after consumer evaluation showed that all red apple ciders, after aging for three months with the addition of yeast (S. bayanus (Lalvin QA23) and S. cerevisiae (Pure Nature, Rubino Cru, White Arome, SIHAFERM Finesse Red)), were attractive in terms of aroma at ≥4.5 units. In turn, red apple cider after fermentation received the lowest score for consistency at ≥3.1 units. The most desirable taste turned out to be that of the ciders fermented with the addition of S. cerevisiae: Rubino Cru (around 4.8 units) and SIHAFERM Finesse Red (around 4.5 units). According to the consumers, the least acceptable in terms of taste after storage was red apple cider fermented with S. bayanus Lalvin C yeast (around 3.1 units). After the sensory evaluation of taste, aroma, consistency, and color, the best cider produced from red apples turned out to be that fermented by S. cerevisiae Rubino Cru and SIHAFERM Finesse Red. It was probably associated with a lesser degree of polymerization (DP) and, hence, lower bitterness. In turn, Nurgel et al. [41] stated that the taste and color are important distinguishing factors taken into account in the consumer assessment of wine. The use of yeasts influenced the protection of an attractive red color, and the protection of compounds with health-promoting effects.

Principal Component Analysis (PCA)
Results of the PCA revealed correlations between organic acid content, values of basic physicochemical parameters, polyphenolic compound content, and antioxidative activity of stored new red apple cider fermented with various yeast strains ( Figure 3). The two principal components (PCs) explained 72.60% of the total variance of tested data, including 51.58% reported for PC1 and 21.06% for PCPC1 was primarily accountable for the disparity between contents of anthocyanins, flavonols, phenolic acids, flavan-3-ols, dihydrochalcones, polymeric procyanidin, organic acids (mainly oxalic and fumaric acids), and antioxidative activity determined with the ABTS assay. In turn, PC2 coupled antioxidative potency with total acidity, as well as contents of organic acids and alcohol. This statistical analysis proved differences between the cider samples as affected by yeast strain and BCD addition during fermentation. The highest concentration of phenolic acids of all groups and of oxalic and fumaric acids in red apple ciders fermented with S. cerevisiae SIHAFERM Finesse Red with the addition of BCD described before depicted a strong positive correlation with the antioxidative potency assessed mainly with the ABTS test. Moreover, the high value of the color parameters a* and b* was responsible for the high proportion of red and yellow colors in these red apple ciders. The use of yeasts confirmed the protective effect of the color of the final product before and after storage, as well as the content of polyphenolic compounds with pro-health effects. Furthermore, high concentrations of organic acids (mainly malic, quinic, and succinic acids) and of alcohol in red apple ciders fermented with S. cerevisiae White Aroma and S. bayanus Lalvin QA23 and Lalvin C were strongly correlated with FRAP capacity. In contrast, in red apple ciders fermented with S. cerevisiae Pure Nature and Rubin Cru, the low content of phenolic compounds and high content of citric, acetic, and maleic acids, as well as the sum of these acids, demonstrated a positive correlation with contents of biologically active compounds and antioxidative properties. Additionally, these ciders were characterized by a high value of parameter L*, which means that they were the brightest. Alberti et al. [13] used PCA analysis to evaluate all variables in green apple beverages and ciders. They reported differences between the tested components and products. There were much more volatile compounds in ciders than amino acids, while an opposite trend was observed in beverages. In addition, the results showed that apple cultivar had a significant impact on the chemical composition of the final ciders made from green apples.

Cider Production
The study involved the red apple of the "Bella Marii" cultivar. The material was collected from a Grzegorz Maryniowski BioGrim company in Wojciechów (51 • 10 22" north (N), 23 • 03 27" east (E), Poland), near Lublin (Poland), in the harvested red apples were fully mature.
The red apples were crushed with a Thermomix (Vorwerk, Wuppertal, Germany). The crushed bulk was pressed with a hydraulic press (TOYA, Wrocław, Poland). The must was supplemented with K 2 S 2 O 5 (POCh Gliwice, Poland) at 0.10 g/L, nutrients (SIHA Proferm Plus) at 0.10 g/L (Eaton, Begerow, Langenlonsheim, Germany), and S. cerevisiae (SIHAFERM Pure Nature, SIHA Rubino Cru, SIHA White Arome, SIHAFERM Finesse Red) and S. bayanus (Lalvin C, Lalvin QA 23 YSEO) at 0.20 g/L. Sample 7 was without potassium metabisulfite, but with BCD added at 1.0 g/L with SIHAFERM Finesse Red at 0.20 g/L. The fermentation was conducted at 20 • C for 15 days. Afterward, the red apple cider was stored at 4 • C for three months.

Physical Analysis
Titratable acidity and pH were determined by titration aliquots (Schott Titroline 7500 KF Volumetric KF Titrator; Mainz, Germany), performed according to the protocol described by Lachowicz et al. [42]. Turbidity was measured using a method described previously by Lachowicz et al. [42]. Ethanol content in wine was determined using an oscillating densimeter DMA 4500 M (Anton Paar, Graz, Austria), with results presented as the volume percentage (vol.%). Results are reported as the arithmetic mean of three independent repetitions, taking into account the standard deviation (SD).

Qualitative and Quantitative Assessment of Polyphenols
All analyses of polyphenols in the tested samples were carried out using an ACQUITY Ultra-Performance LC system (UPLC) equipped with a binary solvent manager (Waters Corp., Milford, MA, USA), a UPLC ethylene-bridged hybrid (BEH) C18 column (1.7 µm, 2.1 mm × 50 mm, Waters Corp., Milford, MA, USA), and a QToF Micro mass spectrometer (Waters, Manchester, UK) with an electrospray ionization (ESI) source operating in negative and positive modes. The analysis was carried out using full-scan, data-dependent MS scanning from m/z 100 to Leucine enkephalin was used as the reference compound at a concentration of 500 pg/µL, and a flow rate of 2 µL/min, and the [M − H] − ion at 554.2615 Da was detected. The [M − H] − ion was detected during 15-min analysis performed within ESI MS accurate mass experiments, which were permanently introduced via the LockSpray channel using a Hamilton pump. The lock mass correction was ± 1.000 for the mass window. The mass spectrometer was operated in the negative-ion mode, set to the base peak intensity (BPI) chromatograms, and scaled to 12,400 counts per second (cps) (100%). The optimized MS conditions were as follows: capillary voltage of 2500 V, cone voltage of 30 V, source temperature of 100 • C, dissolution temperature of 300 • C, and dissolution gas (nitrogen) flow rate of 300 L/h. Collision-induced fragmentation experiments were performed using argon as the collision gas, with voltage ramping cycles from 0.3 to 2 V. The data obtained from UPLC-MS were subsequently entered into the MassLynx 4.0 ChromaLynx Application Manager software. A protocol described earlier by Lachowicz et al. [42] was followed during the extraction and determination of phenolic compounds. The mobile phase consisted of solvent A (4.5% formic acid, v/v) and solvent B (100% acetonitrile). The runs were monitored at the following wavelengths: phenolic acids at 320 nm, flavonols at 340 nm, anthocyanins at 520 nm, and flavan-3-ols at 280 nm. The photodiode array detector (PDA) spectra were measured over the wavelength range of 200-600 nm in steps of 2 nm. The results were expressed as mg/L.

Analysis of Proanthocyanidins via Phloroglucinolysis Method
Phloroglucinolysis products were separated on a Cadenza CD-C18 (75 mm × 4.6 mm, 3 µm) column (Imtakt, Kyoto, Japan). The liquid chromatograph was a Waters (Milford, MA, USA) system equipped with diode array, scanning fluorescence detectors (Waters 474), and an autosampler (Waters 717 plus). Solvent A (25 mL of acetic acid and 975 mL of water) and solvent B (acetonitrile) were used in the following gradients: initial, 5 mL/100 mL B; 0-15 min, to 10 mL/100 mL B linear; 15-25 min to 60 mL/100 mL B linear; followed by washing and reconditioning of the column. A flow rate of 1 mL/min and an oven temperature of 15 • C were used with the injection of the filtrate (20 µL) into the HPLC system. The fluorescence detection was recorded at an excitation wavelength of 278 nm and an emission wavelength of 360 nm. The calibration curves were established using (+)-catechin and (−)-epicatechin (phloroglucinol adduct standards). All data were obtained in triplicate. The results were expressed as mg/L.

Determination of Antioxidative Activity
The free-radical scavenging activities were determined using two methods, ABTS and FRAP (ferric reducing antioxidative power). The ABTS and FRAP assays were conducted as previously described by Re et al. [43] and Benzie and Strain [44], respectively. Determinations by ABTS and FRAP methods were performed using a UV-2401 PC spectrophotometer (Shimadzu, Kyoto, Japan). The antioxidative activity was evaluated by measuring the variation in absorbance at 734 nm after 6 min for ABTS, and at 593 nm after 10 min for FRAP. All antioxidative activity analyses were done in triplicate, and results were expressed as mmol of Trolox equivalent (TE) per L of sample.

Organic Acids
Organic acids were determined by HPLC-PDA as described previously by Wojdyło et al. [45]. All data were obtained in triplicate. Results were expressed as mg/L of red apple cider.

Color Measured
Color properties (L*, a*, b*) of cider from red apple were determined by reflectance measurements with a Color Quest XE Hunter Lab colorimeter. The samples were determined according to the method described by Lachowicz et al. [42]. The data were means of three measurements. The total change in cider before and after storage was expressed as ∆E, and the chroma difference (∆C) [42] was also calculated.

Sensory Attributes
The sensory properties of obtained red apple cider before and after three months of storage at 4 • C were evaluated using a five-degree hedonic scale with boundary indications: "I do not like it very much" (1) to "I like it very much" (5). The assessment included the following quality attributes: taste, aroma, color, and consistency. It was conducted by a group of 20 consumer panelists (10 men and 10 women in the age group of 20-65). Coded samples were provided to the panelists for the evaluation at 20 • C in uniform 50-mL plastic containers.

Statistical Analysis
Statistical analysis was conducted using Statistica version 12.5 (StatSoft, Krakow, Poland). Significant differences (p < 0.05) between means were evaluated by one-way ANOVA, PCA (principal components analysis), and Duncan's multiple range test. All analyses were done in triplicate.

Conclusions
This work provides information on the impact of different yeast strains, addition of BCD, and storage time on the physicochemical properties, antioxidative potential, and final quality of red apple ciders.
Seven variants of red apple ciders were obtained, depending on the yeast strain (S. cerevisiae and S. bayanus), BCD addition, and aging time. Generally, storage at 4 • C had an effect on the quality of the tested components of red apple ciders. Yeast strain and addition of BCD influenced their variety. S. cerevisiae, especially SIHAFERM Finesse Red with BCD and SIHAFERM Finesse Red strain, allowed producing a red apple cider of better physicochemical properties and antioxidative potential than that produced with S. bayanus. SIHAFERM Finesse Red yeast of the new generation influenced the stability and ensured the protection of color and polyphenols, especially anthocyanins, therefore they are recommended for the fermentation of red apple ciders. The addition of BCD affected the protection of polyphenolic compounds, which exhibit the health-promoting properties, and also influenced basic chemical parameters, organic acids, and color of the ciders, nevertheless the taste of these ciders was unacceptable.