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Molecules 2018, 23(7), 1637; https://doi.org/10.3390/molecules23071637

Genistein Improves Skin Flap Viability in Rats: A Preliminary In Vivo and In Vitro Investigation

1
Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Košice, Slovakia
2
Department of Biomedical Research, East-Slovak Institute of Cardiovascular Diseases, 040 11 Košice, Slovakia
3
2nd Clinic of Surgery, Louis Pasteur University Hospital and Pavol Jozef Šafárik University, 041 90 Košice, Slovakia
4
Department of Pharmacognosy and Botany, Comenius University, Faculty of Pharmacy, 832 32 Bratislava, Slovakia
5
Clinic of Infectology and Travel Medicine, Louis Pasteur University Hospital and Pavol Jozef Šafárik University, 041 90 Košice, Slovakia
6
Clinic of Surgery, 1st Private Hospital Košice-Šaca and Pavol Jozef Šafárik University, 040 15 Košice, Slovakia
7
Clinic of Heart Surgery, East-Slovak Institute of Cardiovascular Diseases and Pavol Jozef Šafárik University, 040 11 Košice, Slovakia
8
Clinic of Obstetrics and Gynecology, 1st Private Hospital Košice-Šaca and Pavol Jozef Šafárik University, 040 15 Košice, Slovakia
9
Department of Medical Informatics, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Košice, Slovakia
10
Clinic of Angiology, East-Slovak Institute of Cardiovascular Diseases and Pavol Jozef Šafárik University, 04011 Košice, Slovakia
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 8 June 2018 / Revised: 28 June 2018 / Accepted: 30 June 2018 / Published: 4 July 2018
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Abstract

Selective estrogen receptor modulators (SERMs) have been developed to achieve beneficial effects of estrogens while minimizing their side effects. In this context, we decided to evaluate the protective effect of genistein, a natural SERM, on skin flap viability in rats and in a series of in vitro experiments on endothelial cells (migration, proliferation, antioxidant properties, and gene expression profiling following genistein treatment). Our results showed that administration of genistein increased skin flap viability, but importantly, the difference is only significant when treatment is started 3 days prior the flap surgery. Based on our in vitro experiments, it may be hypothesized that the underlying mechanism may rather by mediated by increasing SOD activity and Bcl-2 expression. The gene expression profiling further revealed 9 up-regulated genes (angiogenesis/inflammation promoting: CTGF, CXCL5, IL-6, ITGB3, MMP-14, and VEGF-A; angiogenesis inhibiting: COL18A1, TIMP-2, and TIMP-3). In conclusion, we observed a protective effect of genistein on skin flap viability which could be potentially applied in plastic surgery to women undergoing a reconstructive and/or plastic intervention. Nevertheless, further research is needed to explain the exact underlying mechanism and to find the optimal treatment protocol. View Full-Text
Keywords: wound healing; skin repair; ischemia; estrogen; flap surgery; endothelial cell; SOD; CAT; Bcl-2 wound healing; skin repair; ischemia; estrogen; flap surgery; endothelial cell; SOD; CAT; Bcl-2
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Fáber, L.; Kováč, I.; Mitrengová, P.; Novotný, M.; Varinská, L.; Vasilenko, T.; Kello, M.; Čoma, M.; Kuruc, T.; Petrová, K.; Miláčková, I.; Kuczmannová, A.; Peržeľová, V.; Mižáková, Š.; Dosedla, E.; Sabol, F.; Luczy, J.; Nagy, M.; Majerník, J.; Koščo, M.; Mučaji, P.; Gál, P. Genistein Improves Skin Flap Viability in Rats: A Preliminary In Vivo and In Vitro Investigation. Molecules 2018, 23, 1637.

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