Production and Bioactivity-Guided Isolation of Antioxidants with α-Glucosidase Inhibitory and Anti-NO Properties from Marine Chitinous Materials

Natural and bioactive products have been of great interest due to their benefit as health foods and drugs to prevent various diseases. The aim of this study is to efficiently reuse marine chitinous materials (CMs), abundant and low-cost fishery by-products, for the bio-synthesis, isolation, and identification of antioxidant compounds possessing some other beneficial bioactivities. Paenibacillus sp. was used to convert CMs to antioxidants. Among various tested CMs, squid pen powder (SPP) gave the best results when used as the sole carbon/nitrogen source. Fermented SPP (FSPP) had comparable antioxidant activity (IC50 = 124 µg/mL) to that of α-tocopherol (IC50 = 30 µg/mL). The antioxidant productivity increased 1.83-fold after culture optimization. The use of multiple techniques, including Diaion, silica, and preparative HPLC columns coupled with a bioassay resulted in the isolation of two major antioxidants characterized as exopolysaccharides and homogentisic acid. These isolated compounds showed great maximum activity and low IC50 values (96%, 30 µg/mL and 99%, 5.4 µg/mL, respectively) which were comparable to that of α-tocopherol (95%, 24 µg/mL). The crude sample, fractions, and isolated compounds also demonstrated α-glucosidase inhibition and anti–inflammatory activity. Notably, homogentisic acid was found as a non-sugar-based moiety α-glucosidase inhibitor which show much higher inhibition (IC50 = 215 µg/mL) than that of acarbose (IC50 = 1324 µg/mL) and also possessed acceptable anti–inflammatory activity (IC50 = 9.8 µg/mL). The results highlighted the value of using seafood processing by-products, like squid pens, for the production of several compounds possessing multi-benefit bioactivities and no cytotoxicity.


Introduction
Living organisms are negatively affected by free radicals [1]. Synthetic and natural antioxidants have been used to prevent damage, but synthetic antioxidants may result in liver damage and

Antioxidant Production by Paenibacillus and Chitinolytic and/or Proteolytic Enzyme-Producing Strains
To determine the best bacterial strain to induce active antioxidants with SPP, a total of nine bacterial strains were tested, including five strains of Paenibacillus and four stains of chitinolytic and/or proteolytic enzyme-producing bacteria. The results in Table 1 show that all tested strains of Paenibacillus had the same potency of antioxidant production. Fermented SPP showed maximum activity (83-94%) and IC50 values (124-169 µg/mL) that were comparable to α-tocopherol (95%, 30 µg/mL). Conversely, the four chitinolytic and/or proteolytic enzyme-producing bacterial strains demonstrated weak antioxidant production with low maximum activity (44-57%) and high IC50 values (2600-3500 µg/mL).
SPP, a fishery processing by-product, was reported to contain 40% chitin and 60% protein [23]. This material was used as the sole C/N source for fermentation in this experiment. Four chitinolytic and/or proteolytic enzyme-producing bacterial strains were used to ferment SPP. Unexpectedly, all tested strains showed weaker activity than Paenibacillus. Among the over 350 bacterial strains isolated from Taiwanese soil, Paenibacillus sp. TKU036 was reported to have the highest antioxidant activity at 85% [11]. However, Paenibacillus sp. TKU042 showed even higher activity (94%) than that of the TKU036 strain, and also showed the highest activity among the nine strains tested in this study. As such, this bacterium was chosen for further investigation. Squid pens, an abundant and low-cost seafood processing by-product [22], have been used for the production of numerous bioactive materials by fermentation. SPP has been extensively studied for the production of chitin and chitosan [23], chitooligomers, and enzymes [23,24], exopolysaccharides [3,25,26], and biosorbents [27,28]. However, aside from the previous studies [3,11], few reports have focused on using SPP to produce antioxidant compounds. As such, it is of great economic value to investigate reusing this low-cost material for antioxidant production.

Antioxidant Production by Paenibacillus and Chitinolytic and/or Proteolytic Enzyme-Producing Strains
To determine the best bacterial strain to induce active antioxidants with SPP, a total of nine bacterial strains were tested, including five strains of Paenibacillus and four stains of chitinolytic and/or proteolytic enzyme-producing bacteria. The results in Table 1 show that all tested strains of Paenibacillus had the same potency of antioxidant production. Fermented SPP showed maximum activity (83-94%) and IC 50 values (124-169 µg/mL) that were comparable to α-tocopherol (95%, 30 µg/mL). Conversely, the four chitinolytic and/or proteolytic enzyme-producing bacterial strains demonstrated weak antioxidant production with low maximum activity (44-57%) and high IC 50 values (2600-3500 µg/mL).
SPP, a fishery processing by-product, was reported to contain 40% chitin and 60% protein [23]. This material was used as the sole C/N source for fermentation in this experiment. Four chitinolytic and/or proteolytic enzyme-producing bacterial strains were used to ferment SPP. Unexpectedly, all tested strains showed weaker activity than Paenibacillus. Among the over 350 bacterial strains isolated from Taiwanese soil, Paenibacillus sp. TKU036 was reported to have the highest antioxidant activity at 85% [11]. However, Paenibacillus sp. TKU042 showed even higher activity (94%) than that of the TKU036 strain, and also showed the highest activity among the nine strains tested in this study. As such, this bacterium was chosen for further investigation.

Optimization of Cultivation Time and Effect of Supplementary Air on Antioxidant Production
To determine optimal cultivation time, 100 mL of medium containing 1% SPP was fermented in a 250 mL Erlenmeyer flask for nine days. Cultivation was performed under two different sets of conditions: with supplementary air given once a day and with no supplementary air. Culture solutions were harvested daily and tested for antioxidant activity and bacterial growth.
Activity detected in the original culture supernatants (undiluted) are expressed as percent in Figure 2A. Under both sets of conditions, activity reached the maximum (approximately 94%) on day 3 with no increase thereafter. There was no significant difference in activity between the two cultures. To clarify the results, the original culture supernatants were diluted 25 times with methanol before activity was tested and expressed as U/mL ( Figure 2B). Again, activity was equal under both sets of conditions. However, since the highest antioxidant production (≥75 U/mL) was achieved on day 4 of fermentation, four days was set as the optimal time for all subsequent experiments.

Optimization of Cultivation Time and Effect of Supplementary Air on Antioxidant Production
To determine optimal cultivation time, 100 mL of medium containing 1% SPP was fermented in a 250 mL Erlenmeyer flask for nine days. Cultivation was performed under two different sets of conditions: with supplementary air given once a day and with no supplementary air. Culture solutions were harvested daily and tested for antioxidant activity and bacterial growth.
Activity detected in the original culture supernatants (undiluted) are expressed as percent in Figure 2A. Under both sets of conditions, activity reached the maximum (approximately 94%) on day 3 with no increase thereafter. There was no significant difference in activity between the two cultures. To clarify the results, the original culture supernatants were diluted 25 times with methanol before activity was tested and expressed as U/mL ( Figure 2B). Again, activity was equal under both sets of conditions. However, since the highest antioxidant production (≥ 75 U/mL) was achieved on day 4 of fermentation, four days was set as the optimal time for all subsequent experiments.
Bacterial growth was also recorded daily ( Figure 2C). The proportional correlation between antioxidant production and bacterial growth under both sets of conditions was only clearly observed from day 1 to day 3 of cultivation. After day 3, activity increased until day 4 and was stable thereafter. Conversely, bacterial growth did not increase after day 3 when supplementary air was given once a day and even dramatically decreased after day 3; the bacterial community died completely by day 7 of cultivation when no supplementary air was given. For convenience, fermentation without supplementary air was chosen for subsequent experiments. Bacterial growth was also recorded daily ( Figure 2C). The proportional correlation between antioxidant production and bacterial growth under both sets of conditions was only clearly observed from day 1 to day 3 of cultivation. After day 3, activity increased until day 4 and was stable thereafter. Conversely, bacterial growth did not increase after day 3 when supplementary air was given once a day and even dramatically decreased after day 3; the bacterial community died completely by day 7 of cultivation when no supplementary air was given. For convenience, fermentation without supplementary air was chosen for subsequent experiments.
In the previous study [22], Paenibacillus sp. TKU042 also induced aGIs after four days of cultivation, but there were significant differences in aGI production depending on whether supplementary air was given. Fermentation with no supplementary air resulted in much greater activity (three-fold higher on day 4) than when supplementary air was provided.

Optimization of Parameters to Achieve Greater Antioxidant Productivity
Other cultivation parameters, including temperature, medium volume, SPP concentration and volume of seed culture, were investigated to obtain greater antioxidant productivity. Paenibacillus sp. TKU042 induced highest antioxidant productivity (≥83 U/mL) at 34-37 • C ( Figure 3A). In order to save energy, 34 • C was chosen while investigating the optimal cultivation medium volume. The results in Figure 3B show that cultivation of Paenibacillus sp. TKU042 in 50-70 mL of medium resulted in much higher antioxidant productivity (≥137 U/mL) than in 100-200 mL (≥60 U/mL). Taking the recovered culture supernatant volume into consideration, a medium volume of 70 mL was chosen for subsequent experiments ( Figure 3C). Antioxidant production was highest with SPP concentrations in the range of 1-2%. SPP at 1% was suggested as the most suitable concentration for Paenibacillus sp. TKU042 to effectively induce antioxidants, and also saved on materials. Therefore, 1% SPP was chosen for subsequent experiments to investigate the effects of bacterial volume on antioxidant production. As shown in Figure 3D, this factor had no effect. No matter the amount of bacteria, antioxidant production remained the same (approximately 130 U/mL).
Overall, the optimal conditions for efficiently inducing antioxidants by Paenibacillus sp. TKU042 were 70 mL culture medium with an initial pH of 6.85, containing 1% SPP, 0.1% K 2 HPO 4 , and 0.05% MgSO 4 ·7H 2 O in a 250 mL Erlenmeyer flask. Cultivation was performed at 34 • C and 150 rpm (shaking speed), with no supplementary air for four days. Activity increased 1.83-fold after optimization (75-137 U/mL).
In the previous study, Paenibacillus sp. TKU036 was also reported to produce antioxidants using the same C/N of SPP. However, SPP fermented by Paenibacillus sp. TKU036 gave the weaker activity (85%) than that of Paenibacillus sp. TKU042 (94%) after three days of fermentation. In addition, antioxidants produced from SPP via Paenibacillus sp. TKU042 could be obtained with greater volume scale (70 mL) in a lower cultivation temperature (34 • C) than those (50 mL, 37 • C) of Paenibacillus sp. TKU036 [11].

Isolation and Identification of Major Active Compounds from Fermented Product
Various techniques, including Diaion, silica opened columns, preparative HPLC column coupled with bioassay, and total sugars detection assays, were applied for partial purification and identification of the major active compounds from FSPP.

Isolation and Identification of Major Active Compounds from Fermented Product
Various techniques, including Diaion, silica opened columns, preparative HPLC column coupled with bioassay, and total sugars detection assays, were applied for partial purification and identification of the major active compounds from FSPP.
The non-EPS antioxidant (FSPP-2.8) was further purified by using preparative HPLC column. Three components of FSPP-2.8.1, FSPP-2.8.2, and FSPP-2.8.3 were obtained and tested for their activity. As presented in Figure 4D, FSPP-2.8.1 and FSPP-2.8.2 showed weak or no activity. In contrast, FSPP-2.8.3 demonstrated strong activity with maximum inhibition and IC 50 values of 99%, and 8.1 µg/mL, respectively. To obtain greater purity of this potent component (FSPP-2.8.3), it was further separated by the same pre-HPLC column and recycled five times. Activity was enhanced 1.5-fold after this final purification step. The analysis of NMR data (1H NMR, 13C NMR) coupled with the comparison to that of the reported compound led to the identification of purified FSPP-2.8.3 as homogentisic acid (HGA) [11]. compounds are EPS antioxidants. This potential fraction was further sub-fractionated by loading it onto a silica opened column and eluting continuously with methanol in dichloromethane (MeOH in DM) a gradient elution from 0/100-100/0. Twenty-one sub-fractions (FSPP-1 to FSPP-21) were collected and tested for antioxidant activity and total sugar content. The results illustrated in Figure  4B showed that two components (FSPP-2.8 and FSPP-2.13) possessed much stronger antioxidant activity than the FSPP-2 fraction, with values of 95%, 92%, and 25%, respectively. FSPP-2.8 contained very few sugars (total sugar content was ≤ 4%) while the total sugar content of FSPP-2.13 was 83%. Therefore, FSPP-2.13 and FSPP-2.8 were characterized as EPS and non-EPS antioxidants, respectively.   Figure 4E.

D Concentration (µg/mL)
HGA is reported to possess valuable medicinal properties, such as antioxidant and anti-inflammatory activities [11], and plays a vital role in phenylalanine and tyrosine metabolism [36]. Recently, HGA was found to be an effective non-sugar-based α-glucosidase inhibitor [22]. Furthermore, this active compound was reported to be produced by only a few bacterial strains of Aspergillus [37], Vibrio cholerae [38], Yarrowia lipolytica [39], and Paenibacillus sp. TKU036 [11].
The EPS antioxidant FSPP-2.13 was further characterized their chemical bonds using enzymatic assays [3]. A total of 0.5 U/mL cellulase, pectinase, and α-amylase were used to hydrolyze FSPP-2.13. After 24 h of hydrolysis, the products (reducing sugars) were detected by measuring OD 540nm after mixing with dinitrosalicylic acid and heating at 100 • C for 10 min. Reducing sugars were only present in the FSPP-2.13 solution hydrolyzed by α-amylase. This result indicated that this EPS contains mostly α-1,4 glycosidic bonds rather than β-1,4 glycosidic bonds [3]. However, there was not enough proof to determine the sugar compositions and molecular weight of this EPS in this study. EPSs are also reported to possess other valuable bioactivities with promising applications in health food, pharmaceuticals, and other industries [25].

Cytotoxicity, Anti-Imflammation and α-Glucosidase Inhibition of Crude Sample, Fraction, and Isolated Compounds
To confirm this fermented product and its isolated compounds as the good source of functional food with multi valuable and no toxicity, the cytotoxicity, α-glucosidase inhibition, and anti-inflammation were tested. As shown in Table 3, all the test samples, including crude sample, fraction, and isolated compounds, showed no cytotoxicity due to their great cell viability value of 91.42-103.05% at high concentration (80 µg/mL) of the tested samples.
α-Glucosidase inhibitory activity was commonly used for evaluation of in vitro anti-diabetic activity [21,22]. The samples were tested at various concentration then the activity was expresses as IC 50 value. Acarbose, a commercial anti-diabetic drug was tested for the comparison. The isolated EPS show no activity (Table 3). However, the crude sample (FSPP), fraction (FSPP-2), and isolated compound (HGA) demonstrated stronger activity that that of positive control with the IC 50 values of 275, 457, 215, and 1324 µg/mL, respectively.
It was reported that nitric oxide (NO) is considered as a mediator of pro-inflammatory activity involved in certain inflammatory disorders, such as pulmonary fibrosis, chronic hepatitis, and rheumatoid arthritis [40]. Anti-inflammation was determined by using LPS-stimulated RAW 264.7 cells as cell line models and presented in Figure 5. All tested samples showed activity with the value in the range of 16.7-92.1% at their concentration of 80 µg/mL. Of these, HGA demonstrated the strongest activity (92.1%). Quercetin, a positive anti-NO was tested for the comparison and showing higher activity (IC 50 = 1.24 µg/mL) than HGA (IC 50 = 9.8 µg/mL). These results suggest that FSPP could be developed as a potent functional food as it contains some compounds of HGA and EPSs possessing multi-benefit bioactivities and no toxicity. Table 3. Cytotoxicity and α-glucosidase inhibition of FSPP, FSSP-2, and its isolated compounds.

Components
Cell

Materials
Squid pens, crab shells, and shrimp shells were acquired from Shin-Ma Frozen Food Co. (I-Lan, Taiwan). Shrimp head powder (SHP) was purchased from Fwu-Sow Industry (Taichun, Taiwan). Fresh shrimp shells were dried with lyophilization then ground to powder (FSSP). Demineralized crab shell powder (deCSP) and demineralized shrimp shell powder (deSSP) were prepared from crab shell and shrimp shell powders as per the methods described by Wang  In the previous study [24], chitooligomers with degrees of polymerization, varying from 3-9, possessing antioxidant and anti-inflammatory activities were reported, and production and isolation from chitosan hydrolyzed by a Bacillus mycoides chitosanase showed lower anti-inflammatory activity (with the IC 50 values in the range of 76.27-82.65 µg/mL) than HGA isolated from FSPP in this study (IC 50 = 9.8 µg/mL). HGA was also reported to be isolated and identified from SPP fermented by Paenibacillus sp. TKU036 in a recent report [11], showing the same anti-inflammatory activity (IC 50 = 10.14 µg/mL) to that of isolated HGA in the current report.

Materials
Squid pens, crab shells, and shrimp shells were acquired from Shin-Ma Frozen Food Co. (I-Lan, Taiwan). Shrimp head powder (SHP) was purchased from Fwu-Sow Industry (Taichun, Taiwan). Fresh shrimp shells were dried with lyophilization then ground to powder (FSSP). Demineralized crab shell powder (deCSP) and demineralized shrimp shell powder (deSSP) were prepared from crab shell and shrimp shell powders as per the methods described by Wang et al., 2006 [41]. Nutrient broth and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) were purchased from Creative Life Science Co. (Taipei, Taiwan) and Sigma Chemical Co. (St. Louis City, MO, USA), respectively. The highest grade of solvents and common chemicals available were used.

Bioactivity Assays
Antioxidant activity was determined using DPPH radical scavenging activity assay. One-hundred microliters (100 µL) of the samples (diluted 25 times with methanol) was mixed with 25 µL 0.75 mM DPPH in methanol in a 96-well plate. The optical density of the mixture solution was measured at 517 nm after being kept in the dark for 30 min [24]. Antioxidant activity was determined using the following formula: where A stands for the optical density of the blank sample at initialization and B the tested sample solution at 30 min. The positive control was α-tocopherol dissolved in MeOH. IC 50 value was defined as the concentration of an antioxidant that could reduce 50% of the DPPH purple color under assay conditions. The activity was also expressed as U/mL, where one U was defined as the volume of the sample required to inhibit 50% DPPH radicals [24]. Cytotoxicity and anti-inflammation assays were also carried out according to the methods described by   [24] with modifications. The cytotoxicity of the samples was tested against murine RAW 264.7 monocyte/macrophage cell lines utilizing the MTT colorimetric protocol. Cells were cultivated in MEM medium. The samples with volume of 20 µL was placed in each well and incubated at 37 • C for 72 h after seeding cells in a 96-well microplate for 4 h, thereafter, 20 µL of MTT was added for 4 h. After the medium was washed off and DMSO (200 µL/well) was added to the microplate with mechanical shaking for 30 min. The formazan crystals were then re-dissolved and measured their absorbance at a wavelength of 550 nm. Quercetin was used as the positive control. α-Glucosidase inhibition was performed as the methods described by Nguyen et al., (2018) [9].

Optimization of Cultivation Time and Effect of Supplementary Air on Antioxidant Production
One gram of SPP was mixed with 100 mL of base mineral medium containing 0.1% K 2 HPO 4 and 0.05% MgSO 4 ·7H 2 O (initial pH 6.85) in a 250 mL Erlenmeyer flask. The prepared medium was fermented by Paenibacillus sp. TKU042. Fermentation was performed at 30 • C with a 150 rpm shaking speed for one to nine days. At the same time, cultivation was carried out under two sets of conditions: with supplementary air once per day or without any supplementary air. Air was supplemented by the methods described in detail by Nguyen et al., 2017 [22]. The culture supernatants were harvested daily and centrifuged at 4000 rpm for 20 min (used to detect activity) and at 500 rpm for 10 min (used to detect bacterial growth by measuring the optical density at 660 nm).

Enzymatic Assays, Total Sugars, and Reducing Sugars Detection
Cellulase, pectinase and α-amylase assays and total sugars detection were performed, as described in the previous study [3]. Reducing sugar was determined following the methods described in the previous report [42].

Statistical Analysis
The differences between the mean values of antioxidant activity, enzyme inhibition, cell viability, and anti-NO (p < 0.01) were analyzed by using Statistical Analysis Software (SAS-9.4, provided by SAS Institute Taiwan Ltd., Minsheng East Road, Section 2, Taipei, Taiwan 149-8). All the tests were repeated in triplicate.

Conclusions
SPP was investigated as the best potential C/N source for antioxidant production by Paenibacillus sp. TKU042 among various chitinous materials. All tested Paenibacillus species showed the same manner of efficient conversion of SPP to antioxidants. Antioxidant production increased 1.83-fold after optimization of cultivation conditions. Antioxidant activity was also dramatically increased by purification. HGA (purified FSPP-2.8.3) and EPS antioxidants (FSPP-2.13) isolated from FSPP showed comparable, or even much higher, activity than that of α-tocopherol with IC 50 values of 5.4, 30, and 24.1 µg/mL, respectively. The crude sample, fraction, and isolated compounds showed stronger α-glucosidase inhibition than that of acarbose. Anti-inflammatory activity was also detected. Of the tested samples, purified HGA demonstrated the most potent activity (92.1%, IC 50 = 9.8 µg/mL). These results suggest that squid pens are valuable for bioactive materials production via bacterial conversion and this fermented product (FSPP) could be developed as healthy foods as it contains major compounds (HGA and EPSs) possessing multi-benefit bioactivities and no toxicity in in vitro testing.