Synthesis of O-Amino Sugars and Nucleosides

Nucleic acids and carbohydrates are essential biomolecules involved in numerous biological and pathological processes. Development of multifunctional building blocks based on nucleosides and sugars is in high demand for the generation of novel oligonucleotide mimics and glycoconjugates for biomedical applications. Recently, aminooxyl-functionalized compounds have attracted increasing research interest because of their easy derivatization through oxime ligation or N-oxyamide formation reactions. Various biological applications have been reported for O-amino carbohydrate- and nucleoside-derived compounds. Here, we report our efforts in the design and synthesis of glyco-, glycosyl, nucleoside- and nucleo-aminooxy acid derivatives from readily available sugars and amino acids, and their use for the generation of N-oxyamide-linked oligosaccharides, glycopeptides, glycolipids, oligonucleosides and nucleopeptides as novel glycoconjugates or oligonucleotide mimics. Delicate and key points in the synthesis will be emphasized.


Introduction
The high nucleophilicity of hydroxylamine derivatives as well as the unique structural and chemical properties of the N-O, oxime and N-oxyamide bonds have triggered the O-amino functionalization of biomolecules like peptides, proteins, carbohydrates, nucleosides and nucleotides. Due to repulsion between the lone pairs of the electron of the nitrogen and oxygen atoms, the N-O bond of hydroxylamine derivatives has unusual conformational properties, which have been exploited to obtain particular N-O turn structures in aminooxy acid-derived peptides for the preparation of new foldamers, anion receptors or channels [1,2]. The N-O bond is relatively sensitive to radical conditions because of the low N-O bond dissociation energy [3]. Oximes show higher hydrolytic stability than hydrazones [4], while the N-oxyamide linkage is resistant to chemical hydrolysis [1]. Aminooxylated carbohydrates and glycoconjugates are attracting the increasing interest for the understanding of the glycobiology and for diverse biomedical applications [3,5]. N-, O-glycosyl hydroxylamines as well as N-hydroxyamino and O-amino sugars have been developed over the last twenty years. Various chemically modified oligonucleotides have also been reported, with an increasing number showing up as drug candidates for oligonucleotide therapies [6][7][8][9]. Among them, O-amino nucleosides have been prepared for the development of human UDP-GlcNAc 4-epimerase inhibitors [10], modified oligonucleotides [11] or for next generation sequencing [12]. N-O, N-oxyamide or N-alkoxycarbamate-linked oligonucleotides have also been synthesized as oligonucleotides mimics [13][14][15][16][17]. The focus of this article is to summarize our own efforts in the preparation of O-amino sugar and nucleoside derivatives, including sugar aminooxy acids, glycosyl α-aminooxy acids, O-aminooxy nucleosides and nucleo-aminooxy acids for the generation of N-oxyamide-linked oligosaccharides, glycopeptides, glycolipids, and oligonucleoside mimics.

Sugar Aminooxy Acids for Oligosaccharide Mimics
The design and synthesis of novel molecules bearing different functions are of great interest for organic, bioorganic, medicinal as well as material chemistry. The creation of cleverly functionalized molecules has a large impact in designing bioactive molecules and for drug discovery. Apart from their biological involvement, carbohydrates are readily available chiral compounds which have been widely used in organic synthesis. We have decided to introduce both aminooxyl and carboxyl functions on the sugar pyranosidic or furanosidic skeletons to generate sugar aminooxy acids or glycoaminooxy acids as multifunctional building blocks which could be used for the synthesis of novel N-oxyamide linked oligosaccharides or glycoconjugates with interesting structural properties. It has been shown that N-oxyamide-linked peptides can easily form intramolecular hydrogen bonds to facilitate turns and helical structures [1,2]. A rigid ribbon-like secondary structure has been observed on a trimer of a cis-β-furanosidic sugar aminooxy acid by the group of Chandrasekhar [18]. Nanorod formation through intermolecular H-bonding has also been observed on a symmetric cyclotetrapeptide prepared from a 2-(C-furanosyl) β-amino acid and an α-aminooxy acid [19]. Besides, the N-oxyamide-linkage is resistant to chemical and enzymatic hydrolysis [20], and N-oxyamide bonds could be readily formed using classical amide formation methods.
We have synthesized D-ribofuranosidic glycoaminooxy acid 4 from diisopropylidene D-glucose (1, Scheme 1) [21]. Since initial efforts to introduce an aminooxyl group at the 3-position of 1 failed, probably due to the steric hindrance, we converted 1 into its homologue 2 through Collins oxidation, Wittig reaction, and hydroboration-hydroxylation reactions. Unlike the secondary alcohol function in 1, the primary alcohol function in 2 reacted efficiently under Mitsunobu conditions with N-hydroxyphthalimide to give 3 after selective deprotection of the 5,6-O-isopropylidene moiety. Oxidative cleavage of the diol, followed by oxidation of the resulting aldehyde, afforded the desired phthalimidooxyl acid 4 which can be readily converted into aminooxy ester 5.

Sugar Aminooxy Acids for Oligosaccharide Mimics
The design and synthesis of novel molecules bearing different functions are of great interest for organic, bioorganic, medicinal as well as material chemistry. The creation of cleverly functionalized molecules has a large impact in designing bioactive molecules and for drug discovery. Apart from their biological involvement, carbohydrates are readily available chiral compounds which have been widely used in organic synthesis. We have decided to introduce both aminooxyl and carboxyl functions on the sugar pyranosidic or furanosidic skeletons to generate sugar aminooxy acids or glycoaminooxy acids as multifunctional building blocks which could be used for the synthesis of novel N-oxyamide linked oligosaccharides or glycoconjugates with interesting structural properties. It has been shown that N-oxyamide-linked peptides can easily form intramolecular hydrogen bonds to facilitate turns and helical structures [1,2]. A rigid ribbon-like secondary structure has been observed on a trimer of a cis-β-furanosidic sugar aminooxy acid by the group of Chandrasekhar [18]. Nanorod formation through intermolecular H-bonding has also been observed on a symmetric cyclotetrapeptide prepared from a 2-(C-furanosyl) β-amino acid and an α-aminooxy acid [19]. Besides, the N-oxyamide-linkage is resistant to chemical and enzymatic hydrolysis [20], and N-oxyamide bonds could be readily formed using classical amide formation methods.
We have synthesized D-ribofuranosidic glycoaminooxy acid 4 from diisopropylidene D-glucose (1, Scheme 1) [21]. Since initial efforts to introduce an aminooxyl group at the 3-position of 1 failed, probably due to the steric hindrance, we converted 1 into its homologue 2 through Collins oxidation, Wittig reaction, and hydroboration-hydroxylation reactions. Unlike the secondary alcohol function in 1, the primary alcohol function in 2 reacted efficiently under Mitsunobu conditions with Nhydroxyphthalimide to give 3 after selective deprotection of the 5,6-O-isopropylidene moiety. Oxidative cleavage of the diol, followed by oxidation of the resulting aldehyde, afforded the desired phthalimidooxyl acid 4 which can be readily converted into aminooxy ester 5. Scheme 1. Synthesis of glycofuranosidic aminooxy acids.
Concerning pyranosidic glycoaminooxy acids, we have firstly prepared the compound 14 bearing the carboxylic acid on the C-glycosidic anomeric chain (Scheme 3) [22]. This compound could be easily prepared from the readily available C-allyl glucoside 13 containing a free hydroxyl group at the C-6 position [23]. Hence, compound 13 was converted to the phthaloyl-protected glycoaminooxy acid 14 in two steps involving Mitsunobu reaction with PhthNOH, followed by oxidation of the alkene moiety to a carboxylic acid. The compound 14 was further transformed into the building block The building blocks 4 and 5 were coupled to form oligomers from di-to hexamers (Scheme 2). For the synthesis of oligomers with more than three sugar units, the tBu group was selectively removed using 13.7% trifluoroacetic acid (TFA) in CH 2 Cl 2 without affecting the acetonide protecting groups.
Concerning pyranosidic glycoaminooxy acids, we have firstly prepared the compound 14 bearing the carboxylic acid on the C-glycosidic anomeric chain (Scheme 3) [22]. This compound could be easily prepared from the readily available C-allyl glucoside 13 containing a free hydroxyl group at the C-6 Molecules 2018, 23, 641 3 of 15 position [23]. Hence, compound 13 was converted to the phthaloyl-protected glycoaminooxy acid 14 in two steps involving Mitsunobu reaction with PhthNOH, followed by oxidation of the alkene moiety to a carboxylic acid. The compound 14 was further transformed into the building block 15 via protection of the carboxylic acid as a tBu-ester followed by the removal of the phthaloyl group to afford the free oxyamine. To demonstrate the synthetic utility of the building blocks 14 and 15 in the preparation of oligosaccharide and glycopeptide mimetics, compound 15 was coupled with 14 or Boc-Gly-OH to give the disaccharide 16 or glycosyl amino acid 17 in good yield.  The pyranosidic glycoaminooxy acid building blocks bearing the aminooxyl and carboxylic groups on the 2,6-positions were prepared from the D-glucopyranoside 18 (Scheme 4) [24]. Alkylation of the hydroxyl group with BrCH2CO2tBu followed by acidic hydrolysis of the tert-butyldimethylsilyl (TBS) group and conversion of the resultant hydroxyl to phthalimidooxyl group afforded the fully protected glycoaminooxy acid 19. Selective removal of the tBu group using TFA in CH2Cl2 formed the building block 20 bearing a free carboxylic acid. On the other hand, the phthaloyl group was removed through hydrazinolysis to the building block 21. The two orthogonally protected glycoaminooxy acid units were employed for the preparation of oligomers from dimer 22 to tetramer 24. The pyranosidic glycoaminooxy acid building blocks bearing the aminooxyl and carboxylic groups on the 2,6-positions were prepared from the D-glucopyranoside 18 (Scheme 4) [24]. Alkylation of the hydroxyl group with BrCH2CO2tBu followed by acidic hydrolysis of the tert-butyldimethylsilyl (TBS) group and conversion of the resultant hydroxyl to phthalimidooxyl group afforded the fully protected glycoaminooxy acid 19. Selective removal of the tBu group using TFA in CH2Cl2 formed the building block 20 bearing a free carboxylic acid. On the other hand, the phthaloyl group was removed through hydrazinolysis to the building block 21. The two orthogonally protected glycoaminooxy acid units were employed for the preparation of oligomers from dimer 22 to tetramer 24. The pyranosidic glycoaminooxy acid building blocks bearing the aminooxyl and carboxylic groups on the 2,6-positions were prepared from the D-glucopyranoside 18 (Scheme 4) [24]. Alkylation of the hydroxyl group with BrCH 2 CO 2 tBu followed by acidic hydrolysis of the tert-butyldimethylsilyl (TBS) group and conversion of the resultant hydroxyl to phthalimidooxyl group afforded the fully protected glycoaminooxy acid 19. Selective removal of the tBu group using TFA in CH 2 Cl 2 formed the building block 20 bearing a free carboxylic acid. On the other hand, the phthaloyl group was removed through hydrazinolysis to the building block 21. The two orthogonally protected glycoaminooxy acid units were employed for the preparation of oligomers from dimer 22 to tetramer 24. From our results and those reported in the literature [3,5], we can conclude that oligomers derived from sugar aminooxy acids can be obtained as efficiently as those formed from the previously developed sugar amino acid building blocks [25][26][27]. However, benzyl protecting groups are to be avoided due to the sensitivity of the N-O bond towards the benzyl-deprotecting hydrogenolysis conditions [3].
We then decided to use acid-sensitive para-methoxybenzyl groups (Scheme 5) [28]. First, methyl α-D-glucopyranoside was selectively protected with TBS and para-methoxybenzyl (PMB) groups to 25 and 26. Contrary to compound 18, the 2-O-alkylation of 26 has necessitated the optimization of the reaction conditions due to the very low conversion. The best conversion (64%) was obtained by slow addition of BrCH2CO2Et after formation of the alcoolate. The phthalimidooxyl group has been introduced under Mitsunobu conditions after desilylation, leading to the PMB-protected glycoaminooxy ester 28. The efficient deprotection of the PMB has been demonstrated in the corresponding glycolipids mimics (vide infra). Scheme 5. Synthesis of pyranosidic glycoaminooxy ester 28.

Glycosyl α-Aminooxy Acid Derivatives
Glycosyl amino acids are constituents of glycopeptides and glycoproteins playing key roles in biological processes. We have synthesized C-and O-glycosyl aminooxy acid derivatives as novel glycosyl amino acid building blocks for the generation of glycopeptide mimics. The α-C-glycosyl aminooxy acid derivatives can be readily prepared from α-C-allyl glucosides 29 and 30, through dihydroxylation, selective tritylation, Mitsunobu reaction with PhthNOH, detritylation and oxidation [29] (Scheme 6). Both α-C-glucosyl L-and D-α-aminooxy esters 35-38 have been successfully synthesized. From our results and those reported in the literature [3,5], we can conclude that oligomers derived from sugar aminooxy acids can be obtained as efficiently as those formed from the previously developed sugar amino acid building blocks [25][26][27]. However, benzyl protecting groups are to be avoided due to the sensitivity of the N-O bond towards the benzyl-deprotecting hydrogenolysis conditions [3].
We then decided to use acid-sensitive para-methoxybenzyl groups (Scheme 5) [28]. First, methyl α-D-glucopyranoside was selectively protected with TBS and para-methoxybenzyl (PMB) groups to 25 and 26. Contrary to compound 18, the 2-O-alkylation of 26 has necessitated the optimization of the reaction conditions due to the very low conversion. The best conversion (64%) was obtained by slow addition of BrCH 2 CO 2 Et after formation of the alcoolate. The phthalimidooxyl group has been introduced under Mitsunobu conditions after desilylation, leading to the PMB-protected glycoaminooxy ester 28. The efficient deprotection of the PMB has been demonstrated in the corresponding glycolipids mimics (vide infra). From our results and those reported in the literature [3,5], we can conclude that oligomers derived from sugar aminooxy acids can be obtained as efficiently as those formed from the previously developed sugar amino acid building blocks [25][26][27]. However, benzyl protecting groups are to be avoided due to the sensitivity of the N-O bond towards the benzyl-deprotecting hydrogenolysis conditions [3].
We then decided to use acid-sensitive para-methoxybenzyl groups (Scheme 5) [28]. First, methyl α-D-glucopyranoside was selectively protected with TBS and para-methoxybenzyl (PMB) groups to 25 and 26. Contrary to compound 18, the 2-O-alkylation of 26 has necessitated the optimization of the reaction conditions due to the very low conversion. The best conversion (64%) was obtained by slow addition of BrCH2CO2Et after formation of the alcoolate. The phthalimidooxyl group has been introduced under Mitsunobu conditions after desilylation, leading to the PMB-protected glycoaminooxy ester 28. The efficient deprotection of the PMB has been demonstrated in the corresponding glycolipids mimics (vide infra). Scheme 5. Synthesis of pyranosidic glycoaminooxy ester 28.

Glycosyl α-Aminooxy Acid Derivatives
Glycosyl amino acids are constituents of glycopeptides and glycoproteins playing key roles in biological processes. We have synthesized C-and O-glycosyl aminooxy acid derivatives as novel glycosyl amino acid building blocks for the generation of glycopeptide mimics. The α-C-glycosyl aminooxy acid derivatives can be readily prepared from α-C-allyl glucosides 29 and 30, through dihydroxylation, selective tritylation, Mitsunobu reaction with PhthNOH, detritylation and oxidation [29] (Scheme 6). Both α-C-glucosyl L-and D-α-aminooxy esters 35-38 have been successfully synthesized.

Glycosyl α-Aminooxy Acid Derivatives
Glycosyl amino acids are constituents of glycopeptides and glycoproteins playing key roles in biological processes. We have synthesized Cand O-glycosyl aminooxy acid derivatives as novel glycosyl amino acid building blocks for the generation of glycopeptide mimics. The α-C-glycosyl aminooxy acid derivatives can be readily prepared from α-C-allyl glucosides 29 and 30, through dihydroxylation, selective tritylation, Mitsunobu reaction with PhthNOH, detritylation and oxidation [29] (Scheme 6). Both α-C-glucosyl L-and D-α-aminooxy esters 35-38 have been successfully synthesized.  With a reliable method to both stereoisomers of the β-O-glucosylserine analogues in hand, the synthesis of N-oxyamide-linked glycopeptides was investigated (Scheme 8). The glucosyl tripeptide mimetic 46 was prepared from 42 after coupling with FmocGlyOH, deprotection of the tBu group, and coupling with GlyOtBu. The O-amino function can also be protected with Fmoc group and the corresponding glycopeptide 47 could be further used in the solid-phase peptide synthesis. Interestingly, NMR and IR studies suggested that compound 46 formed an α N-O turn structure via an eight-membered-ring intramolecular H bond between the amide NH and the carbonyl group of the N-oxyamide.  With a reliable method to both stereoisomers of the β-O-glucosylserine analogues in hand, the synthesis of N-oxyamide-linked glycopeptides was investigated (Scheme 8). The glucosyl tripeptide mimetic 46 was prepared from 42 after coupling with FmocGlyOH, deprotection of the tBu group, and coupling with GlyOtBu. The O-amino function can also be protected with Fmoc group and the corresponding glycopeptide 47 could be further used in the solid-phase peptide synthesis. Interestingly, NMR and IR studies suggested that compound 46 formed an α N-O turn structure via an eight-membered-ring intramolecular H bond between the amide NH and the carbonyl group of the N-oxyamide.

N-Oxyamide-Linked Glycolipids
As part of the glycoconjugate family, glycolipids are implicated in a variety of important biological phenomena such as cell-cell interactions, viral and bacterial infections, immune responses, signal transduction, cell proliferation, etc. Natural glycolipids like glycoglycerolipids (GGLs) and glycosphingolipids (GSLs), as well as their synthetic mimics, have also shown very interesting biological activities [31,32]. The design of glycolipid mimics has become a useful strategy in drug discovery. We have prepared N-oxyamide-linked glycolipids as mimics of glycoglycerolipids and glycosphingolipids by replacing the ester group in GGL with a N-oxyamide functionality [33,34]. The βglucolipids bearing two lipid chains 53-57 were synthesized from the O-phthaloylamino β-glucoglycerol 41, by esterification with palmitic acid, removal of the phthaloyl group and coupling with different carboxylic acids so as to introduce a lipid chain onto the nitrogen atom, and final full deprotection with hydrazine (Scheme 9). Three β-glucolipids bearing one lipid chains 61-63 have also been obtained from 40. Coupling of octanoic acid with the glucosyl aminooxy ester 42 led to glucolipid 64 [30].

N-Oxyamide-Linked Glycolipids
As part of the glycoconjugate family, glycolipids are implicated in a variety of important biological phenomena such as cell-cell interactions, viral and bacterial infections, immune responses, signal transduction, cell proliferation, etc. Natural glycolipids like glycoglycerolipids (GGLs) and glycosphingolipids (GSLs), as well as their synthetic mimics, have also shown very interesting biological activities [31,32]. The design of glycolipid mimics has become a useful strategy in drug discovery. We have prepared N-oxyamide-linked glycolipids as mimics of glycoglycerolipids and glycosphingolipids by replacing the ester group in GGL with a N-oxyamide functionality [33,34]. The β-glucolipids bearing two lipid chains 53-57 were synthesized from the O-phthaloylamino β-glucoglycerol 41, by esterification with palmitic acid, removal of the phthaloyl group and coupling with different carboxylic acids so as to introduce a lipid chain onto the nitrogen atom, and final full deprotection with hydrazine (Scheme 9). Three β-glucolipids bearing one lipid chains 61-63 have also been obtained from 40. Coupling of octanoic acid with the glucosyl aminooxy ester 42 led to glucolipid 64 [30].

N-Oxyamide-Linked Glycolipids
As part of the glycoconjugate family, glycolipids are implicated in a variety of important biological phenomena such as cell-cell interactions, viral and bacterial infections, immune responses, signal transduction, cell proliferation, etc. Natural glycolipids like glycoglycerolipids (GGLs) and glycosphingolipids (GSLs), as well as their synthetic mimics, have also shown very interesting biological activities [31,32]. The design of glycolipid mimics has become a useful strategy in drug discovery. We have prepared N-oxyamide-linked glycolipids as mimics of glycoglycerolipids and glycosphingolipids by replacing the ester group in GGL with a N-oxyamide functionality [33,34]. The βglucolipids bearing two lipid chains 53-57 were synthesized from the O-phthaloylamino β-glucoglycerol 41, by esterification with palmitic acid, removal of the phthaloyl group and coupling with different carboxylic acids so as to introduce a lipid chain onto the nitrogen atom, and final full deprotection with hydrazine (Scheme 9). Three β-glucolipids bearing one lipid chains 61-63 have also been obtained from 40. Coupling of octanoic acid with the glucosyl aminooxy ester 42 led to glucolipid 64 [30]. Synthesis of galactoglycerolipids 73-77 has been realized from D-galactose pentaacetate in a similar way as the gluco-derivatives (Scheme 10) [34]. Interestingly, deacetylation of 68 and 69 under Zemplén conditions gave the intramolecular transacylation products 78 and 79, while the compound 71 led to the galactolipid 80. The synthesized glycolipids bearing one and two palmitic acyl chains 56, 62, 76 and 80 are able to assemble with polyethyleneglycol (PEG) thiol-coated gold nanoparticles to form a new type of glyco-Au nanoparticle (AuNP) capable of receptor-targeting cell imaging and drug delivery. The galactolipids derived galacto-AuNPs are selective to galactose-selective peanut agglutinin (PNA), while the gluco-AuNPs selective for concanavalin A (Con A). The efficiency of 76-AuNP for receptor-targeting hepatocellular imaging of human liver cancer Hep-G2 and the anticancer drug hydrocamptothecin delivery has been demonstrated.

O-Aminooxy Nucleosides
We have designed nucleoside aminooxy acids as novel building blocks for the development of stable oligonucleotide mimetics [35]. Replacement of the ionic phosphodiester by the neutral N-oxyamide linkage would not only increase its cellular permeability but also enhance its resistance to extra-and intracellular nucleases degradation. Synthesis of modified RNA oligomers has attracted renewed interest since the discovery of small interfering RNA (siRNA) for gene regulation [6]. We are particularly interested in the development of ribonucleoside aminooxy acid which could be further used for the generation of N-oxyamide-linked oligoribonucleosides. From the previously prepared furanosidic glycoaminooxy acid

O-Aminooxy Nucleosides
We have designed nucleoside aminooxy acids as novel building blocks for the development of stable oligonucleotide mimetics [35]. Replacement of the ionic phosphodiester by the neutral N-oxyamide linkage would not only increase its cellular permeability but also enhance its resistance to extra-and intracellular nucleases degradation. Synthesis of modified RNA oligomers has attracted renewed interest since the discovery of small interfering RNA (siRNA) for gene regulation [6]. We are particularly interested in the development of ribonucleoside aminooxy acid which could be further used for the generation of N-oxyamide-linked oligoribonucleosides. We then decided to investigate the stability of uridine derivative 86 in the presence of MeOH (Scheme 13). Treatment of 86 with K 2 CO 3 in MeOH at room temperature led to the ring-opening From the diacetone D-glucose 1, nucleoside aminooxy esters 106-108 bearing phthalimidooxyl group on 5'-position have been prepared according to the Scheme 14 [36]. Compound 1 was first converted to allylic ester 104. Mitsunobu reaction of 104 with PhthNOH led to impure 105 which was then prepared through the mesylate intermediate followed by nucleophilic substitution reaction. N-glycosylation in the same conditions as for 4 led to the target nucleosides 106-108. Scheme 14. Synthesis of nucleoside aminooxy esters 106-108.
From the uridine, we have prepared the uridine aminooxy esters through selective 2',5'-di-Osilylation, oxidation of 2'-OH followed by Wittig reaction and hydrogenation under acidic condition to give stereoselectively the ester 109 (Scheme 15) [36]. However, all temptations to introduce the phthalimidooxyl function on 5'-position through the Mitsunobu reaction failed. Hopefully, the 5'-hydroxyl function can be converted to Boc-or phthaloyl protected 110 and 111 through iodide intermediate, then deprotected to oxyamine nucleoside 112. Unfortunately, deprotection of the ester groups in 110 and 111 led to degradation, making further formation of N-oxyamide-linked nucleoside aminooxy acid dimers impossible. We have also tried to protect the oxyamine in 112 with a 4-pentenoyl (Pen) group. Once again, decomposition was observed during the saponification or removal of the Pen-protecting group with I2 [37]. From the uridine, we have prepared the uridine aminooxy esters through selective 2',5'-di-Osilylation, oxidation of 2'-OH followed by Wittig reaction and hydrogenation under acidic condition to give stereoselectively the ester 109 (Scheme 15) [36]. However, all temptations to introduce the phthalimidooxyl function on 5'-position through the Mitsunobu reaction failed. Hopefully, the 5'-hydroxyl function can be converted to Boc-or phthaloyl protected 110 and 111 through iodide intermediate, then deprotected to oxyamine nucleoside 112. Unfortunately, deprotection of the ester groups in 110 and 111 led to degradation, making further formation of N-oxyamide-linked nucleoside aminooxy acid dimers impossible. We have also tried to protect the oxyamine in 112 with a 4-pentenoyl (Pen) group. Once again, decomposition was observed during the saponification or removal of the Pen-protecting group with I2 [37]. From the uridine, we have prepared the uridine aminooxy esters through selective 2',5'-di-O-silylation, oxidation of 2'-OH followed by Wittig reaction and hydrogenation under acidic condition to give stereoselectively the ester 109 (Scheme 15) [36]. However, all temptations to introduce the phthalimidooxyl function on 5'-position through the Mitsunobu reaction failed. Hopefully, the 5'-hydroxyl function can be converted to Boc-or phthaloyl protected 110 and 111 through iodide intermediate, then deprotected to oxyamine nucleoside 112. Unfortunately, deprotection of the ester groups in 110 and 111 led to degradation, making further formation of N-oxyamide-linked nucleoside aminooxy acid dimers impossible. We have also tried to protect the oxyamine in 112 with a 4-pentenoyl (Pen) group. Once again, decomposition was observed during the saponification or removal of the Pen-protecting group with I 2 [37].

Nucleo-Aminooxy Acids
As promising alternatives to peptide nucleic acids, nucleobase-functionalized peptides have attracted increasing interest because of their well-ordered secondary structures and stability towards enzymatic degradations, as well as their cellular nucleus penetration ability without cytotoxic effects [39][40][41]. We have designed and synthesized α-amino-β-aminooxy acid derivatives bearing nucleobases covalently attached to its α-position through an amide or triazole linkage as novel building blocks of nucleopeptides (Scheme 17) [42]. The key aminooxy acid 119 was prepared from L-Ser-OMe. Coupling reaction with carboxybenzyl (Cbz)-protected cytosin-1-ylacetic acid formed successfully the nucleoaminooxy ester 120. However, the analogous reaction with thymin-1-ylacetic acid was found to be problematic because of the difficult purification. The phthaloyl group in 119 was then replaced by a Boc group to give 121 via Cbz-protection of the amino group. The Cbz group in 121 should be

Nucleo-Aminooxy Acids
As promising alternatives to peptide nucleic acids, nucleobase-functionalized peptides have attracted increasing interest because of their well-ordered secondary structures and stability towards enzymatic degradations, as well as their cellular nucleus penetration ability without cytotoxic effects [39][40][41]. We have designed and synthesized α-amino-β-aminooxy acid derivatives bearing nucleobases covalently attached to its α-position through an amide or triazole linkage as novel building blocks of nucleopeptides (Scheme 17) [42]. The key aminooxy acid 119 was prepared from L-Ser-OMe. Coupling reaction with carboxybenzyl (Cbz)-protected cytosin-1-ylacetic acid formed successfully the nucleoaminooxy ester 120. However, the analogous reaction with thymin-1-ylacetic acid was found to be problematic because of the difficult purification. The phthaloyl group in 119 was then replaced by a Boc group to give 121 via Cbz-protection of the amino group. The Cbz group in 121 should be removed using Pd-catalyzed hydrogenolysis in less than 10 min to avoid reductive cleavage of the

Nucleo-Aminooxy Acids
As promising alternatives to peptide nucleic acids, nucleobase-functionalized peptides have attracted increasing interest because of their well-ordered secondary structures and stability towards enzymatic degradations, as well as their cellular nucleus penetration ability without cytotoxic effects [39][40][41]. We have designed and synthesized α-amino-β-aminooxy acid derivatives bearing nucleobases covalently attached to its α-position through an amide or triazole linkage as novel building blocks of nucleopeptides (Scheme 17) [42]. The key aminooxy acid 119 was prepared from L-Ser-OMe. Coupling reaction with carboxybenzyl (Cbz)-protected cytosin-1-ylacetic acid formed successfully the nucleo-aminooxy ester 120. However, the analogous reaction with thymin-1-ylacetic acid was found to be problematic because of the difficult purification. The phthaloyl group in 119 was then replaced by a Boc group to give 121 via Cbz-protection of the amino group. The Cbz group in 121 should be removed using Pd-catalyzed hydrogenolysis in less than 10 min to avoid reductive cleavage of the N-O bond. The thymidine aminooxy ester 122 was obtained in moderate yield because of the over acylation on the NH-O nitrogen. Improved yields were obtained from the bis-Boc-protected 123. Subsequently, full deprotection led to the target nucleo-aminooxy acids 126 and 127. Triazole-linked nucleo aminooxy esters 129 and 130 have been prepared from the azido derivative 128, where the use of ascorbic acid instead of sodium ascorbate is essential to avoid the elimination of the phthalimidooxy moiety during the triazole formation. However, synthesis of the corresponding nucleopeptides was hampered by the rapid elimination reaction of the phthalimidooxyl group to give an acylamidoacrylate derivative during the deprotection of methyl esters in 124, 125, 129 and 130. To avoid this side reaction, we have then decided to prepare nucleo-aminooxy acids based on α-amino-γ-aminooxy ester 132, which can be obtained from N-Cbz-L-Met through S-alkylation with iodoacetamide followed by lactonisation in hot citric acid (Scheme 18) [43]. To remove the Cbz protecting group without the cleavage of the N-O bond, 40% Pd/C (w/w) was necessary to quickly deprotect the Cbz group. Subsequent coupling with functionalized nucleobases afforded the corresponding nucleo γ-aminooxy esters 133-136. However, synthesis of the corresponding nucleopeptides was hampered by the rapid elimination reaction of the phthalimidooxyl group to give an acylamidoacrylate derivative during the deprotection of methyl esters in 124, 125, 129 and 130. To avoid this side reaction, we have then decided to prepare nucleo-aminooxy acids based on α-amino-γ-aminooxy ester 132, which can be obtained from N-Cbz-L-Met through S-alkylation with iodoacetamide followed by lactonisation in hot citric acid (Scheme 18) [43]. To remove the Cbz protecting group without the cleavage of the N-O bond, 40% Pd/C (w/w) was necessary to quickly deprotect the Cbz group. Subsequent coupling with functionalized nucleobases afforded the corresponding nucleo γ-aminooxy esters 133-136. of methyl esters in 124, 125, 129 and 130. To avoid this side reaction, we have then decided to prepare nucleo-aminooxy acids based on α-amino-γ-aminooxy ester 132, which can be obtained from N-Cbz-L-Met through S-alkylation with iodoacetamide followed by lactonisation in hot citric acid (Scheme 18) [43]. To remove the Cbz protecting group without the cleavage of the N-O bond, 40% Pd/C (w/w) was necessary to quickly deprotect the Cbz group. Subsequent coupling with functionalized nucleobases afforded the corresponding nucleo γ-aminooxy esters 133-136. Scheme 18. Synthesis of nucleo γ-aminooxy acid derivatives. Scheme 18. Synthesis of nucleo γ-aminooxy acid derivatives.
As expected, the obtained nucleo γ-aminooxy esters can be saponified to the corresponding carboxylic esters without elimination of the phthalimidooxyl group (Scheme 19). Coupling reaction with the oxyamine derivatives 139-141 furnished the N-oxyamide-linked nucleopeptides 142-144. Improved coupling yield could be obtained by using DMF as solvent to solubilize the starting materials. As expected, the obtained nucleo γ-aminooxy esters can be saponified to the corresponding carboxylic esters without elimination of the phthalimidooxyl group (Scheme 19). Coupling reaction with the oxyamine derivatives 139-141 furnished the N-oxyamide-linked nucleopeptides 142-144. Improved coupling yield could be obtained by using DMF as solvent to solubilize the starting materials.

Conclusions
From readily available sugars like glucose and galactose, as well as the amino acids serine and methionine, we have developed several synthetic approaches to glyco-, glycosyl, nucleoside and nucleo-aminooxy acid derivatives. Thanks to the high reactivity of the oxyamine function, these compounds have been successfully employed as multifunctional scaffolds for the generation of Noxyamide-linked oligosaccharides, glycopeptides, glycolipids, oligonucleosides and nucleopeptides as novel glycoconjugates or oligonucleotide mimics. Furthermore, the synthesized N-oxyamidelinked glycolipids were found to be able to form a new type of glyco-AuNP capable of sugar receptortargeting cell imaging and drug delivery. Synthesis and derivatization of different carbohydrate aminooxy acids works well. For the synthesis of deprotected derivatives, the benzyl protecting group needs to be avoided due to the instability of N-O bonds under radical conditions. Synthesis of nucleoside aminooxy acids from the nucleosides remains challenging because of the difficulty to introduce an aminooxyl function on the sugar moiety and the decomposition problems observed during the deprotection steps. To the best of our knowledge, the N-glycosylation of the furanosidic aminooxy esters remains the only current method to access nucleoside aminooxy esters bearing five different nucleobases. We are convinced that these results should be useful to further development of O-amino sugars and nucleosides with interesting biological applications.
We are convinced that these results should be useful to further development of O-amino sugars and nucleosides with interesting biological applications.