Six Heterocyclic Metabolites from the Myxobacterium Labilithrix luteola

Two new secondary metabolites, labindole A [2-methyl-3-(2-nitroethyl)-3H-indole] (1) and labindole B [2-methyl-3-(2-nitrovinyl)-3H-indole] (2), were isolated from the myxobacterium Labilithrix luteola (DSM 27648T). Additionally, four metabolites 3, 4, 5 and 6 already known from other sources were obtained. Their structures were elucidated from high resolution electrospray ionisation mass spectrometry (HRESIMS) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy data and their relative configuration was assigned based on nuclear Overhauser effect (NOE) and vicinal 1H-NMR coupling data. The compounds where tested for biological activities; labindoles A (1) and B (2) exhibited significant activity against Hepatitis C Virus, 9H-carbazole (3), 3-chloro-9H-carbazole (4) and 4-hydroxymethyl-quinoline (5) showed antifungal activities. Moreover, compound 3 had weak to moderate antibacterial activities, while labindoles A (1) and B (2) were devoid of significant antifungal and antibacterial effects.


Introduction
The global challenge of increased drug resistance has led to strong demand to increase the chemical diversity of antibiotics, especially to obtain drugs that can overcome bacterial resistance through new modes of action [1].The emergence of old viral infections such as Chikungunya, an RNA virus that belongs to the alphavirus genus of the family Togaviridae, a mosquito-borne viral disease first described during an outbreak in southern Tanzania in 1952, as well as Ebola outbreaks [2] and HIV pandemics, call for more research into antiviral drugs.Myxobacteria have emerged to be among the main producers of bioactive molecules, with a high chemical diversity in their unique structures and unusual and often novel modes of action [3].In recent years, various novel carbon skeletons with interesting bioactivities have been isolated from hitherto under-explored taxa such as sorazolones [4] and carolacton [5] from Sorangium cellulosum, argyrins [6] and tubulysins [7] from Archangium gephyra, aetheramides from Aetherobacter spp.[8], disciformycins from Pyxidicoccus fallax [9] and cystobactamids from Cystobacter spp.[10].This indicates that isolation of compounds from uncommon strains, in particular those that belong to unexplored phylogenetic lineages, such as Labilithrix, is of great interest.During the course of our studies on the secondary metabolism of the recently described myxobacterium Labilithrix luteola (DSM 27648 T ) [11], which belongs to the family Labilitrichaceae in suborder Sorangiineae, antimicrobial activities were observed and correlated with some prominent peaks with unique high performance liquid chromatography-diode array/mass spectrometry (HPLC-DAD/MS) characteristics attracted our interest.In the following, the fermentation process was optimized and scaled up, resulting in the discovery of two new and several known compounds.Their isolation and biological and physico-chemical characterization are reported in the current paper.

Results and Discussion
In batches of 500 mL broth in 1 L shake flasks, ten liters of fermentation broth of Labilithrix luteola was incubated at 30 • C in presence of Amberlite XAD 16 (Rohm and Haas, Frankfurt, Germany) absorber resin [12] and harvested after 14 days.The resin was recovered by filtration and eluted with methanol, giving 5 g of crude extract, which was separated by Sephadex LH-20 gel permeation chromatography in methanol.Subsequent preparative reversed-phase HPLC of the LH-20 fractions gave six hetero-aromatic metabolites.
Compound 1 (Figure 1) was obtained as yellow powder and turned bright orange on spraying thin layer chromatography plates with Dragendorff reagent [13].The ultraviolet (UV) absorption maxima at 222 nm and 281 nm suggested the presence of an indole alkaloid.The elemental formula was determined by high resolution electrospray ionisation mass spectrometry (HRESIMS) combined with isotopic pattern analysis of the molecular ion cluster [M + H] + (m/z 205.0968) as C 11 H 12 N 2 O 2 , revealing seven double-bond equivalents (DBE). 13C and 13 C-DEPT (Distortionless Enhancement by Polarization Transfer) NMR spectra in CD 3 OD indicated four quaternary carbons as well as four aromatic methine, two methylene, and one methyl carbon (Table 1).The carbons were correlated to their corresponding 11 protons from an 1 H, 13 C-HSQC spectrum, which left one exchangeable proton.The 1 H, 1 H-COSY-NMR spectrum showed that the four aromatic methines belong to a ortho disubstituted aromatic ring, and the methylene groups were directly connected, while the methyl group only had a long-range correlation with CH 2 -8.These structure elements were connected with the remaining carbons due to their 1 H, 13 C-HMBC correlations to give the carbon skeleton of 1.The 1 H, 15 N-HMBC spectrum revealed a nitrogen signal at δ N = 133.1 correlated to methyl group C-2 and methine C-7 completing an indole group, while the second nitrogen was attached as a nitro group, δ N = 388.24ppm, to methylene C-9 from correlations with C-8 and C-9.Although the chemical shift δ N = 388.24ppm was in the expected range, the presence of the nitro group was further verified by a very strong band at 1556 cm −1 in the IR spectrum and by a fragment ion [M+H-HNO 2 ] + in the HR-ESIMS at m/z 158.0962.Finally, the exchanged NH proton completed the structure of Labindole A (1) as 2-methyl-3-(2-nitroethyl)-1H-indole.
Molecules 2018, 22, x 2 of 9 recently described myxobacterium Labilithrix luteola (DSM 27648 T ) [11], which belongs to the family Labilitrichaceae in suborder Sorangiineae, antimicrobial activities were observed and correlated with some prominent peaks with unique high performance liquid chromatography-diode array/mass spectrometry (HPLC-DAD/MS) characteristics attracted our interest.In the following, the fermentation process was optimized and scaled up, resulting in the discovery of two new and several known compounds.Their isolation and biological and physico-chemical characterization are reported in the current paper.

Results and Discussion
In batches of 500 mL broth in 1 L shake flasks, ten liters of fermentation broth of Labilithrix luteola was incubated at 30 °C in presence of Amberlite XAD 16 (Rohm and Haas, Frankfurt, Germany) absorber resin [12] and harvested after 14 days.The resin was recovered by filtration and eluted with methanol, giving 5 g of crude extract, which was separated by Sephadex LH-20 gel permeation chromatography in methanol.Subsequent preparative reversed-phase HPLC of the LH-20 fractions gave six hetero-aromatic metabolites.
Compound 1 (Figure 1) was obtained as yellow powder and turned bright orange on spraying thin layer chromatography plates with Dragendorff reagent [13].The ultraviolet (UV) absorption maxima at 222 nm and 281 nm suggested the presence of an indole alkaloid.The elemental formula was determined by high resolution electrospray ionisation mass spectrometry (HRESIMS) combined with isotopic pattern analysis of the molecular ion cluster [M + H] + (m/z 205.0968) as C11H12N2O2, revealing seven double-bond equivalents (DBE). 13C and 13 C-DEPT (Distortionless Enhancement by Polarization Transfer) NMR spectra in CD3OD indicated four quaternary carbons as well as four aromatic methine, two methylene, and one methyl carbon (Table 1).The carbons were correlated to their corresponding 11 protons from an 1 H, 13 C-HSQC spectrum, which left one exchangeable proton.The 1 H, 1 H-COSY-NMR spectrum showed that the four aromatic methines belong to a ortho disubstituted aromatic ring, and the methylene groups were directly connected, while the methyl group only had a long-range correlation with CH2-8.These structure elements were connected with the remaining carbons due to their 1 H, 13 C-HMBC correlations to give the carbon skeleton of 1.The 1 H, 15 N-HMBC spectrum revealed a nitrogen signal at δN = 133.1 correlated to methyl group C-2 and methine C-7 completing an indole group, while the second nitrogen was attached as a nitro group, δN = 388.24ppm, to methylene C-9 from correlations with C-8 and C-9.Although the chemical shift δN = 388.24ppm was in the expected range, the presence of the nitro group was further verified by a very strong band at 1556 cm −1 in the IR spectrum and by a fragment ion [M+H-HNO2] + in the HR-ESIMS at m/z 158.0962.Finally, the exchanged NH proton completed the structure of Labindole A (1) as 2-methyl-3-(2-nitroethyl)-1H-indole.Compound 2 was isolated by reverse phase HPLC from another LH-20 chromatography fraction of the raw extract as yellow powder and tested positive to Dragendorff reagent.Analysis of the monoisotopic ion clusters [M + H] − and [M + Na + H] − (203.0814 and 225.0634) suggested the elemental formula C 11 H 10 N 2 O 2 , i.e., a loss of two hydrogens compared to 1.The dehydrogenation was also apparent from the NMR spectra of 2, which had lost the methylene groups, but showed two new methines at δ H/C = 8.35/132.6 ppm and 7.80/131.8ppm (Table 1).Their vicinal coupling constant of J = 13.3Hz indicated a trans configuration of the new ∆ 8,9 double bond in Labindole B (2), i.e., (E)-2-methyl-3-(2-nitrovinyl)-1H-indole.The extracts of Labilithrix luteola (DSM 27648) further contained four hetero-aromatic metabolites previously isolated from other sources, including 9H-carbazole (3) [14], which was previously isolated from root bark of Glycomis pentaphylla [15], while 3-chloro-9H-carbazole (4) was found in bovine urine [16].Interestingly, 4-hydroxymethyl-quinoline (5) was already known from the Myxobacterium Archangium gephyra [17] and from the basidiomycete fungi "Polyporus" (current generic name: Pycnoporus) sanguineus and "Polyporus" (current generic name: Coriolus) versicolor [18] with moderate activity against yeast and filamentous fungi, whereas 3,6-dibenzylpyrazin-2(1H)-one (6) was recently described as a product of the expression of gene clusters from gut bacteria [19].
Although previously reported as chemically derivative compounds, this is the first isolation of the structurally related Labindoles A (1) [20] and B (2) [21] from a natural source.Interestingly, the 2-methylindole residue had previously only been found in 2-methyltryptophan as an intermediate in the biosynthesis of the peptide antibiotic thiostrepton in trapping experiments with Streptomyces laurentii [22].However, an aromatic nitro compound, pyrrolnitrin (7) (Figure 2) has previously been described as a fungistatic antibiotic of the myxobacteria Myxococcus fulvus, Corallococcus exiguus, and Cystobacter ferrugineus [23], and it is also known to be an antifungal agent of pseudomonads [24].Labindoles A (1) and B (2) are very rare examples of primary nitro compounds from bacteria.
Although previously reported as chemically derivative compounds, this is the first isolation of the structurally related Labindoles A (1) [20] and B (2) [21] from a natural source.Interestingly, the 2-methylindole residue had previously only been found in 2-methyltryptophan as an intermediate in the biosynthesis of the peptide antibiotic thiostrepton in trapping experiments with Streptomyces laurentii [22].However, an aromatic nitro compound, pyrrolnitrin (7) (Figure 2) has previously been described as a fungistatic antibiotic of the myxobacteria Myxococcus fulvus, Corallococcus exiguus, and Cystobacter ferrugineus [23], and it is also known to be an antifungal agent of pseudomonads [24].Labindoles A (1) and B (2) are very rare examples of primary nitro compounds from bacteria.The compounds isolated were tested in our screening panel against bacteria, fungi, cell cultures and for antiviral activity against HCV (Hepatitis-C-Virus) in human liver cells.Compound 4 inhibited HCV infectivity very strongly.Compound 3, Labindoles A (1) and B (2) had a statistically significant HCV infectivity inhibition, 5 had a weak activity, while 6 was not active, all showed no cytotoxicity on the liver cells, which was simultaneously determined (Figure 3).
The green tea molecule Epigallocatechin gallate (EGCG) was used as positive control [25].Although 4 has been previously isolated 16 , this is the first time the strong inhibition of HCV infectivity activity has been reported.
The compounds isolated were tested in our screening panel against bacteria, fungi, cell cultures and for antiviral activity against HCV (Hepatitis-C-Virus) in human liver cells.Compound 4 inhibited HCV infectivity very strongly.Compound 3, Labindoles A (1) and B (2) had a statistically significant HCV infectivity inhibition, 5 had a weak activity, while 6 was not active, all showed no cytotoxicity on the liver cells, which was simultaneously determined (Figure 3).
The green tea molecule Epigallocatechin gallate (EGCG) was used as positive control [25].Although 4 has been previously isolated 16 , this is the first time the strong inhibition of HCV infectivity activity has been reported.The assay was performed in quadruplicate (L1-L2) and triplicate (L3-L6) and is presented as the mean ± standard deviation.*** P ≤ 0.05.Huh-7.5.Cells were inoculated with RLuc Jc1 reporter viruses in the presence of different compounds.The inoculum was removed 4 h later and monolayers were washed three times with phosphate buffered saline (PBS) and overlaid with fresh medium containing no inhibitors.Infected cells were lysed 3 days later, and reporter virus infection was determined by renilla luciferase activity.The cell viability was measured by determination of firefly luciferase.Viability assay results are given in supplementary material (Figure S19).
When 1-6 were tested for their cytotoxicity against growing primary and cancer cells lines, they did not show any significant activity (Table 2).All compounds were analyzed for their antimicrobial activity against bacteria and fungi.Labindole A (1), Labindole B (2) and 6 showed no activity, whereas 3, 4, and 5 showed weak antifungal activity in addition 3 had weak activity against Bacillus subtilis and Chromobacter violaceum (Table 3).When 1-6 were tested for their cytotoxicity against growing primary and cancer cells lines, they did not show any significant activity (Table 2).All compounds were analyzed for their antimicrobial activity against bacteria and fungi.Labindole A (1), Labindole B (2) and 6 showed no activity, whereas 3, 4, and 5 showed weak antifungal activity in addition 3 had weak activity against Bacillus subtilis and Chromobacter violaceum (Table 3).were evaporated in a rotatory evaporator, giving 5 g of crude extract.The crude extract divided into five portions and separated using Sephadex LH 20 in methanol, flow rate 8 mL/min.;detection UV at 280 nm (Pharmacia Biotec, Piscataway, NJ, USA).The fractions were combined peak-wise, giving 18 fractions, which were evaporated to dryness.Fraction 14 (15 mg) was further purified by preparative RP-HPLC (Nucleodur, C18, 5 µm column, 250 × 21 mm, water and MeCN solvent system at gradient of 30-100% MeCN in 30 min.; flow rate 20 mL/min, UV detection at 300 nm.Labindole A (1) (10 mg) eluted with a retention time of t R = 29.9min.

Inhibitory Effects on HCV Infectivity
Huh7.5 cells stably expressing Firefly luciferase (Huh7.5 Fluc) were cultured in Dulbecco's modified minimum essential medium (DMEM, Life Technologies, Carlsbad, CA, USA) containing 2 mM glutamine, 1× minimum essential medium nonessential amino acids (MEM NEAA, Life Technologies), 100 µg/mL streptomycin, 100 IU/mL penicillin (Life Technologies), 5 µg/mL blasticidin and 10% fetal bovine serum.Cells were maintained in a 37 • C environment with 5% CO 2 supply.Cells were infected with Jc1-derived Renilla reporter viruses in the presence or absence of compounds as described previously [25].Infected cells were lysed and then frozen at −80 • C for 1 h following measurements of Renilla and Firefly luciferase activities on a Centro XS3 Microplate luminometer (Berthold Technologies, Bad Wildbad, Germany) as indicators of viral genome replication and cell viability, respectively.

Cytotoxic Activity
The cytotoxicity of the test compounds was evaluated by measuring the effect produced on cell morphology, including the nuclei and cell growth in vitro.Cell monolayers were prepared in 24-well tissue culture plates and exposed to various concentrations of the compounds.Plates were checked by light microscopy after 24, 48 and 72 h.Cytotoxicity was scored as morphological alterations (e.g.rounding up, shrinking, detachment and disintegration of nuclei).The viability of the cells was determined by a tetrazolium-based colorimetric method using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), as previously described [26,27].The 50% cytotoxic dose, CD 50 , is the concentration of the compound that reduced the absorbance of the control sample by 50%

Figure 3 .
Figure 3. Hepatitis C Virus (HCV) assay: NC-Negative control, EGCG-Positive control, L1-Labindole A (1), L2-Labindole B (2), L3-L6 compound 3-6.The assay was performed in quadruplicate (L1-L2) and triplicate (L3-L6) and is presented as the mean ± standard deviation.*** P ≤ 0.05.Huh-7.5.Cells were inoculated with RLuc Jc1 reporter viruses in the presence of different compounds.The inoculum was removed 4 h later and monolayers were washed three times with phosphate buffered saline (PBS) and overlaid with fresh medium containing no inhibitors.Infected cells were lysed 3 days later, and reporter virus infection was determined by renilla luciferase activity.The cell viability was measured by determination of firefly luciferase.Viability assay results are given in supplementary material (Figure S19).

Figure 3 .
Figure 3. Hepatitis C Virus (HCV) assay: NC-Negative control, EGCG-Positive control, L1-Labindole A (1), L2-Labindole B (2), L3-L6 compound 3-6.The assay was performed in quadruplicate (L1-L2) and triplicate (L3-L6) and is presented as the mean ± standard deviation.*** P ≤ 0.05.Huh-7.5.Cells were inoculated with RLuc Jc1 reporter viruses in the presence of different compounds.The inoculum was removed 4 h later and monolayers were washed three times with phosphate buffered saline (PBS) and overlaid with fresh medium containing no inhibitors.Infected cells were lysed 3 days later, and reporter virus infection was determined by renilla luciferase activity.The cell viability was measured by determination of firefly luciferase.Viability assay results are given in supplementary material (Figure S19).