Identification and Characterization of Phenylpropanoid Biosynthetic Genes and Their Accumulation in Bitter Melon (Momordica charantia)

Phenylpropanoids and flavonoids belong to a large group of secondary metabolites, and are considered to have antioxidant activity, which protects the cells against biotic and abiotic stresses. However, the accumulation of phenylpropanoids and flavonoids in bitter melon has rarely been studied. Here, we identify ten putative phenylpropanoid and flavonoid biosynthetic genes in bitter melon. Most genes were highly expressed in leaves and/or flowers. HPLC analysis showed that rutin and epicatechin were the most abundant compounds in bitter melon. Rutin content was the highest in leaves, whereas epicatechin was highly accumulated in flowers and fruits. The accumulation patterns of trans-cinnamic acid, p-coumaric acid, ferulic acid, kaempferol, and rutin coincide with the expression patterns of McPAL, McC4H, McCOMT, McFLS, and Mc3GT, respectively, suggesting that these genes play important roles in phenylpropanoid and flavonoid biosynthesis in bitter melon. In addition, we also investigated the optimum light conditions for enhancing phenylpropanoid and flavonoid biosynthesis and found that blue light was the most effective wavelength for enhanced accumulation of phenylpropanoids and flavonoids in bitter melon.


Introduction
Momordica charantia, commonly known as "bitter melon" or "bitter gourd", is a medicinal plant belonging to family Cucurbitaceae.Traditionally, it has been used to treat diseases such as HIV, diabetes, cancer, arthritis, liver disorders, and gastric problems [1][2][3][4][5][6].Researchers have found that the important components of bitter melon with respect to its pharmaceutical application are phenolic acids, flavonoids, triterpenes, and carotenoids [7].Phenolic compounds (such as phenylpropanoids and flavonoids) belong to the largest group of secondary metabolites produced by plants, and are known to have antioxidant activity, protecting the cells against biotic and abiotic stresses including infections, wounding, UV irradiation, exposure to ozone, pollutants, and herbivores [8][9][10].Considering these biological characteristics and functions, phenylpropanoids and/or flavonoids have been studied in many plants such as Fagopyrum esculentum [11,12], Fagopyrum tartaricum [13][14][15], Fragaria ananassa Duch.[16], Morus alba L. [17], Scutellaria baicalensis [18], Scutellaria lateriflora [18], and Astragalus membranaceus [19,20].The phenolic compound content and antioxidant properties of bitter melon were described previously by Horax et al. [21].However, little is known about the genes involved in phenylpropanoid and flavonoid biosynthesis pathways in bitter melon.Phenylpropanoids and flavonoids belong to a large class of plant phenols that are produced through the shikimic acid pathway.The biosynthesis of phenylpropanoids and flavonoids and the various catalyzing enzymes involved are shown in Figure 1.
Molecules 2017, 22, 469 2 of 14 Astragalus membranaceus [19,20].The phenolic compound content and antioxidant properties of bitter melon were described previously by Horax et al [21].However, little is known about the genes involved in phenylpropanoid and flavonoid biosynthesis pathways in bitter melon.Phenylpropanoids and flavonoids belong to a large class of plant phenols that are produced through the shikimic acid pathway.The biosynthesis of phenylpropanoids and flavonoids and the various catalyzing enzymes involved are shown in Figure 1.Recently, irradiation by different wavelengths of light-emitting diodes (LEDs) is being used as source of artificial lighting in plant production systems and is attracting much attention due to its high potential for commercial applications.LEDs have many advantages such as small size, long operating lifetime, wavelength specificity, low heat emission, high energy-conversion efficiency, and adjustable light intensity [22,23].The use of LEDs has also allowed researchers to easily analyze the effects of different wavelengths and light intensities on the accumulation of secondary compounds in controlled environments.Irradiation by different wavelengths of light with light-emitting diodes (LEDs) has been reported to efficiently modulate phenolic and flavonoid biosynthesis in plants such as lettuce [24,25], buckwheat [26,27], and Chinese cabbage [28].However, it has not been reported in bitter melon.
In this study, we identified the genes involved in phenylpropanoid and flavonoid synthesis in bitter melon, using a transcriptome database reported in a previous study [29].We also analyzed the expression profiles of these genes and the accumulation of phenylpropanoids and flavonoids in different organs and plantlets to investigate the relationship between phenylpropanoid and flavonoid contents and their gene expression levels.In addition, we investigated whether white, blue, and red light conditions could enhance phenylpropanoid and flavonoid accumulation in bitter melon.To the best of our knowledge, the present study is the first to apply transcriptome sequence Recently, irradiation by different wavelengths of light-emitting diodes (LEDs) is being used as source of artificial lighting in plant production systems and is attracting much attention due to its high potential for commercial applications.LEDs have many advantages such as small size, long operating lifetime, wavelength specificity, low heat emission, high energy-conversion efficiency, and adjustable light intensity [22,23].The use of LEDs has also allowed researchers to easily analyze the effects of different wavelengths and light intensities on the accumulation of secondary compounds in controlled environments.Irradiation by different wavelengths of light with light-emitting diodes (LEDs) has been reported to efficiently modulate phenolic and flavonoid biosynthesis in plants such as lettuce [24,25], buckwheat [26,27], and Chinese cabbage [28].However, it has not been reported in bitter melon.
In this study, we identified the genes involved in phenylpropanoid and flavonoid synthesis in bitter melon, using a transcriptome database reported in a previous study [29].We also analyzed the expression profiles of these genes and the accumulation of phenylpropanoids and flavonoids in different organs and plantlets to investigate the relationship between phenylpropanoid and flavonoid contents and their gene expression levels.In addition, we investigated whether white, blue, and red light conditions could enhance phenylpropanoid and flavonoid accumulation in bitter melon.To the best of our knowledge, the present study is the first to apply transcriptome sequence analysis in the study of phenylpropanoid and flavonoid gene expression in bitter melon.It marks a first step towards possible bioengineering of bitter melon to increase the yield of these important phenylpropanoids and flavonoids.

Expression of Phenylpropanoid and Flavonoid Genes in Different Organs of Bitter Melon
A previous study showed that approximately 88,703 unigenes have been identified in bitter melon seedlings [29].Among the unigenes from the bitter melon database, we could identify 10 1).These genes were highly similar to those in other species belonging to the Cucurbitaceae, such as Cucumis sativus and Cucumis melo.The expression patterns of these genes were analyzed in roots, stems, young leaves, mature leaves, male flowers, female flowers, and four fruit developmental stages using quantitative RT-PCR (Figure 2). Figure 2A shows that McPAL, McCHS, McCHI, and McDFR were mainly expressed in male flowers.The expression level of McC4H was the highest in young and mature leaves, followed by female flowers and male flowers.Although the level of Mc4CL expression was found to be similarly high in stems, male flowers, and female flowers, it was low in the roots and leaves.McF3H had a higher expression in male flowers, young leaves, and mature leaves than in other organs.McFLS showed the highest levels of expression in mature leaves, followed by young leaves and female flowers, and low expression in roots, stems, and male flowers.McCOMT was highly expressed in both male and female flowers, intermediately expressed in stems, young leaves, and mature leaves, and expressed at low levels in the roots.

Phenylpropanoid and Flavonoid Accumulation in Different Organs of Bitter Melon
Quantification of phenylpropanoid and flavonoid content is shown in Table 2 and Table 3.We found that phenylpropanoids and flavonoids were rarely biosynthesized in the roots, whereas abundant phenylpropanoids and flavonoids were detected in the leaves and flowers of bitter melon (Table 2).The observed distribution of phenylpropanoids and flavonoids was similar to that observed in other plants such as L. chinense [30] and Fagopyrum esculentum [31].The accumulation of 4hydroxybenzoic acid, caffeic acid, p-coumaric acid, and rutin was more concentrated in the leaves than in the other organs, whereas benzoic acid was more abundant in the stems than in the other organs.Rutin was the most abundant component in young and mature leaves (3970.83and 2277.93 μg/g dry weight, respectively), with concentrations 107-and 61-fold than that in the root (37.035 μg/g

Phenylpropanoid and Flavonoid Accumulation in Different Organs of Bitter Melon
Quantification of phenylpropanoid and flavonoid content is shown in Tables 2 and 3. We found that phenylpropanoids and flavonoids were rarely biosynthesized in the roots, whereas abundant phenylpropanoids and flavonoids were detected in the leaves and flowers of bitter melon (Table 2).The observed distribution of phenylpropanoids and flavonoids was similar to that observed in other plants such as L. chinense [30] and Fagopyrum esculentum [31].The accumulation of 4-hydroxybenzoic acid, caffeic acid, p-coumaric acid, and rutin was more concentrated in the leaves than in the other organs, whereas benzoic acid was more abundant in the stems than in the other organs.Rutin was the most abundant component in young and mature leaves (3970.83and 2277.93 µg/g dry weight, respectively), with concentrations 107-and 61-fold than that in the root (37.035 µg/g dry weight), respectively (Table 2).Rutin content has also been reported to be the highest in leaves of Morus alba L. [17] and Lycium chinense [30].The accumulation of gallic acid, catechin hydrate, epicatechin, and trans-cinnamic acid was only found in the flowers.Among phenolic compounds, epicatechin was the most abundant component in female and male flowers (568.67 and 660.35 µg/g dry weight, respectively).The content of chlorogenic acid was considerably higher in male flowers (58.7 µg/g dry weight) and female flowers (49.39 µg/g dry weight) that in the other organs.Ferulic acid content was the highest in female flowers (9.49µg/g dry weight) and male flowers (10.27 µg/g dry weight), whereas only small amounts were found in stems, young leaves, and mature leaves.
Phenylpropanoid and flavonoid content on the developed fruits was similar to that in the four developmental stages (Table 3).The amount of the phenolic compounds varied highly from stage 1 (green color) to stage 4 (orange color).For example, epicatechin and p-coumaric acid were more concentrated in stage 2 than in the other fruit stages.Caffeic acid accumulated only at the first stage of fruit maturation (being 13.91 µg/g dry weight), while kaempferol accumulated only at stages 1 and 4. The accumulation of rutin increased from stage 1 (12.49µg/g dry weight) to stage 3 (20.7 µg/g dry weight), and then decreased again at stage 4 (11.15µg/g dry weight).The chlorogenic acid content increased from stage 1 (37.89µg/g dry weight) to stage 4 (40.47 µg/g dry weight).Similar contents of ferulic acid were found in the four fruit stages (Table 3).

Expression of Phenylpropanoid and Flavonoid Genes in Different Developmental Stages of Bitter Melon Plantlets
Expression analysis for the phenylpropanoid and flavonoid genes was carried out at different developmental stages (0 days after sowing (DAS), 5 DAS, 10 DAS, and 15 DAS) using real-time PCR (Figure 3  The roots, stems, Y-leaves (young leave), M-leaves (mature leaves), F-flowers (female flowers), M-flowers (male flowers), and four fruit stages (stage 1-4) of 3-month-old plants were collected and used for HPLC analysis.Values are expressed as means ± SD (standard deviation) from three independent measurements.Letters a-e indicate significant differences (p < 0.05).Values are expressed as means ± standard deviation from three independent measurements.Letters a-c indicate significant differences (P < 0.05).

Expression of Phenylpropanoid and Flavonoid Genes in Different Developmental Stages of Bitter Melon Plantlets
Expression analysis for the phenylpropanoid and flavonoid genes was carried out at different developmental stages (0 days after sowing (DAS), 5 DAS, 10 DAS, and 15 DAS) using real-time PCR (Figure 3).

Phenylpropanoid and Flavonoid Accumulation in Different Developmental Stages of Bitter Melon Plantlets
HPLC analysis of bitter melon plantlets in different developmental stage identified nine phenylpropanoid and flavonoid components, including caffeic acid, trans-cinnamic acid, ferulic acid, benzoic acid, 4-hydroxybenzoic acid, and chlorogenic acid, p-coumaric acid, catechin hydrate, and rutin, as shown in Table 4.The accumulated amounts ranged from undetectable to 6.79, 4.91 to 5.29, 0.58 to 5.14, 0.66 to 4.61, and 4.14 to 7.92 µg/g dry weight for caffeic acid, ferulic acid, p-coumaric acid, trans-cinnamic acid, and benzoic acid, respectively.The accumulation levels of chlorogenic acid at 0, 10, and 15 DAS were similar, but it was not detectable at 5 DAS.The level of 4-hydroxybenzoic acid was the highest at 10 DAS (40.98 µg/g dry weight), followed by the levels at 15 DAS (18.82 µg/g dry weight) and 0 DAS (0.375 µg/g dry weight), but it was undetectable at 5 DAS.High amounts of rutin were found at 10 and 15 DAS (157.935 and 208.97 µg/g dry weight, respectively), whereas lower amounts were found at 0 DAS (12.27 µg/g dry weight).Finally, high amounts of catechin hydrate were found at 0 and 5 DAS (26.52 and 28.875 µg/g dry weight, respectively), and low amounts were found at 15 DAS (13.12 µg/g dry weight).

Effects of White, Blue, and Red Lights on Phenylpropanoid and Flavonoid Content
The contents of phenolic acids, including caffeic acid, rutin, and kaempferol, were measured in seedlings grown under white, blue, or red light for 7, 14, and 21 days (Table 5).Under all light conditions, the accumulation of rutin was the highest (up to 493.96, 433.9, and 87.47 µg/g dry weight under blue, white, and red light irradiation, respectively), followed by that of caffeic acid and kaempferol.However, the effects of white, blue, or red light illumination on caffeic acid, rutin, and kaempferol content varied.Under white light illumination, caffeic acid content gradually increased from 7 to 21 days.In contrast, the rutin content decreased from 433.9 µg/g dry weight at seven days to 195.62 µg/g dry weight at 21 days, whereas kaempferol content reached a peak level at 14 days, and then declined.Under red light illumination, rutin and kaempferol content decreased by about 3.8-fold and 1.8-fold, respectively, whereas caffeic acid content increased by about 2-fold under red light irradiation.This result was congruent with the high expression of , suggesting that blue light may induce the phelynpropanoid and flavoloid biosynthetic pathways, leading to increased rutin content in bitter melon.Many previous studies have shown that blue light illumination can affect the accumulation of phenolic compounds in plants.For example, blue light irradiation was effective in increasing the concentration of most phenolic compounds, including p-hydroxybenzoic acid, ferulic acid, quercetin, and kaempferol in Chinese cabbage [28].Blue light caused a high accumulation of polyphenol content in lettuce [24].A study by Ki-Ho Son and Myung-Min Oh [25] indicated that the ratio of blue to red LEDs is important for the morphology, growth, and the concentration of phenolic compounds with antioxidant properties in green and red leaf lettuce cultivars.In this study, our analysis established that blue light had the optimum wavelength for phenylpropanoid and flavonoid biosynthesis in bitter melon.However, additional research is required to obtain a more comprehensive assessment.In summary, we identify ten putative biosynthetic enzymes in Momordica charantia that have high homology to enzymes involved in the biosynthetic pathway leading to phenylpropanoid and flavonoid synthesis in other plants.Most genes were highly expressed in leaves and/or flowers.An HPLC analysis showed that rutin and epicatechin were most abundant in bitter melon.The accumulation of rutin was highest in leaves, while epicatechin was high accumulated in flowers and fruits.The accumulation patterns of rutin and epicatechin coincide with the expression pattern of indicating that these genes play important roles in phenylpropanoid and flavonoid biosynthesis in bitter melon.In contrast, the accumulation patterns of benzoic acid did not coincide with the expression pattern of phenylpropanoid and flavonoid biosynthetic genes in Momordica charantia.Other phenylpropanoid and flavonoid biosynthetic genes may be linked to the phenylpropanoid and flavonoid synthetic pathway.In addition, we also investigated optimum white, blue, and red light conditions for enhancing phenylpropanoid and flavonoid biosynthesis and found that blue light was the most effective wavelength to enhance accumulation of phenylpropanoid and flavonoid in bitter melon.These results will be of value for developing strategies to increase the yield of medicinal compounds in bitter melon.

Plant Materials
Bitter melon (Momordica charantia) seeds were purchased from Beijing Namo Tech.-TradeCo. Ltd. (Beijing, China).After peeling, the seeds were surface-sterilized with 70% (v/v) ethanol for 30 s and 1% (v/v) sodium hypochlorite solution for 10 min.The sterilized seeds were germinated using 1/2 MS medium as we described previously [32].After sowing for 0, 5, 10, and 15 days (Figure 5A and Table S1), the plantlets were harvested.For an LED irradiation experiment, after seeding in a growth chamber at 25 • C for 10 days, bitter melon plantlets were transplanted under white (380 nm), blue (470 nm), or red (660 nm) LED irradiation conditions at the Precision Agriculture Lab, Chungnam National University (Daejeon, Korea).They were grown in a plant factory with the same growth conditions of temperature (25 ± 1 • C), humidity (85-90%), electrical conductivity (1200 ± 90 µS/cm), CO 2 (1000 ± 100 ppm), light intensity (80-90 µmol•m −2 •s −1 ), and photoperiod (16-h light/8-h dark) under different LED irradiation treatments over a 21-day period.Whole plantlets were harvested at 7, 14, and 21 days after irradiation (DAI).In addition, bitter melon was grown at an experimental farm at Chungnam National University (Daejeon, Korea) for three months, for harvesting the plant parts (roots, stems, mature leaves, young leaves, male flowers, and female flowers) and fruits at four different stages (Figure 5B and Table S2).Whole cultivations and irradiations were repeated three times.All samples after harvest were immediately frozen in liquid nitrogen and used for RNA isolation and/or high-performance liquid chromatography-ultraviolet (HPLC-UV) analysis.
phenylpropanoid and flavonoid in bitter melon.These results will be of value for developing strategies to increase the yield of medicinal compounds in bitter melon.

Plant Materials
Bitter melon (Momordica charantia) seeds were purchased from Beijing Namo Tech.-TradeCo. Ltd. (Beijing, China).After peeling, the seeds were surface-sterilized with 70% (v/v) ethanol for 30 seconds and 1% (v/v) sodium hypochlorite solution for 10 min.The sterilized seeds were germinated using 1/2 MS medium as we described previously [32].After sowing for 0, 5, 10, and 15 days (Figure 5A and Table S1), the plantlets were harvested.For an LED irradiation experiment, after seeding in a growth chamber at 25 °C for 10 days, bitter melon plantlets were transplanted under white (380 nm), blue (470 nm), or red (660 nm) LED irradiation conditions at the Precision Agriculture Lab, Chungnam National University (Daejeon, Korea).They were grown in a plant factory with the same growth conditions of temperature (25 ± 1 °C), humidity (85%-90%), electrical conductivity (1,200 ± 90 μS/cm), CO2 (1000 ± 100 ppm), light intensity (80-90 μmolm −2 s −1 ), and photoperiod (16-h light/8-h dark) under different LED irradiation treatments over a 21-day period.Whole plantlets were harvested at 7, 14, and 21 days after irradiation (DAI).In addition, bitter melon was grown at an experimental farm at Chungnam National University (Daejeon, Korea) for three months, for harvesting the plant parts (roots, stems, mature leaves, young leaves, male flowers, and female flowers) and fruits at four different stages (Figure 5B and Table S2).Whole cultivations and irradiations were repeated three times.All samples after harvest were immediately frozen in liquid nitrogen and used for RNA isolation and/or high-performance liquid chromatography-ultraviolet (HPLC-UV) analysis.

RNA Isolation and cDNA Synthesis
Total RNA was isolated from the different plant parts using Easy BLUE Total RNA Kit (iNtRON, Seongnam, Korea), and the quality was confirmed by running a 1.2% agarose gel at 100 W power. ReverTra Ace-kit (Toyobo Co. Ltd, Osaka, Japan) procured from Japan was used for the synthesis of cDNA from 1 μg of total RNA.The prepared cDNA was then diluted 20 times prior to performing quantitative real-time PCR.

RNA Isolation and cDNA Synthesis
Total RNA was isolated from the different plant parts using Easy BLUE Total RNA Kit (iNtRON, Seongnam, Korea), and the quality was confirmed by running a 1.2% agarose gel at 100 W power. ReverTra Ace-kit (Toyobo Co. Ltd, Osaka, Japan) procured from Japan was used for the synthesis of cDNA from 1 µg of total RNA.The prepared cDNA was then diluted 20 times prior to performing quantitative real-time PCR.

Quantitative Real-Time PCR Analysis
The primers of 10 genes involved in the phenylpropanoid and flavonoid pathways were designed using the Primer3 website [33] (details provided in Table S3).For qRT-PCR, the reaction mixture (20 µL) contained primers (0.5 µM), diluted cDNA (10-fold), PCR mixture of 2X SYBR Green (10 µL), and water (5 µL).The PCR machine was run for 3 min (95 • C), 15 s (95 • C, 41 cycles), 15 s (56 • C), and for a final extension of 20 s (72 • C).The cyclophilin gene (Accession number: HQ171897.1)was used as the positive gene reference for quantifying the expression of the selected genes.The PCR products were analyzed using the Bio-Rad CFX Manager 2.0 software (Bio-Rad Laboratories, Hercules, CA, USA).The analysis was repeated three times.

High Performance Liquid Chromatography (HPLC) Analysis
Phenylpropanoid and flavonoid compounds were quantified using an HPLC connected to a C18 column (250 mm × 4.6 mm, 5 µm).The samples were mixed with 80% (v/v) ethanol and incubated at 25 • C for 60 min to complete the extraction of compounds.The samples were eluted in the HPLC system with an elution buffer consisting of methanol and water: acetic acid (98.5:1.5 v/v) at 1 mL/min −1 .The levels of phenylpropanoid and flavonoid compounds were calculated based on the peak area of the standard compounds and the calibration curve.Quantification and analysis were performed in triplicate.

Statistical Analysis
Statistical analysis was performed with Statistical Analysis System (SAS version 9.2, SAS Institute Inc., Cary, NC, USA, 2009) using an analysis of variance (ANOVA) with Duncan's honestly significant difference test.All data are the mean values and standard deviation of triplicate experiments.

Conclusions
This study provides the first description of the correlation between the putative phenylpropanoid and flavonoid biosynthetic genes and the altered phenylpropanoid and flavonoid contents found in different organs and plantlets of bitter melon.We identified and isolated the cDNAs for 10  indicating that these genes play important roles in phenylpropanoid and flavonoid biosynthesis in bitter melon.In addition, we also investigated white, blue, and red light conditions with respect to their effect on the accumulation of phenylpropanoids and flavonoids in bitter melon.The results of this study demonstrate that blue light is an effective light source for the enhancement of phenylpropanoid and flavonoid biosynthesis.These results will be of value for developing strategies to increase the yield of medicinal compounds in bitter melon.

Supplementary Materials:
The following are available online, Table S1: The development indicators of plantlet of bitter melon.Data represents mean values ± SE (standard error) of three replicates.DAS-days after sowing, Table S2: The size of four developmental fruit stages of bitter melon.Data represents mean values ± SE of three replicates, Table S3: Sequences of specific primers used for quantitative real-time PCR.

14 BFigure 2 .
Figure 2. Expression levels of genes involved in phenylpropanoid and flavonoid biosynthesis pathway in different organs (A) and four fruit stages (stage 1-4) (B) of bitter melon plants.The vertical axes show the expression relative to cyclophilin and the height of each bar and the error bars indicate the mean and standard error, respectively, from three independent measurements.Y-leaves: young leave; M-leaves: mature leaves; F-flowers: female flowers; M-flowers: male flowers.Letters a−e indicate significant differences (p < 0.05).

Figure 2 .
Figure 2. Expression levels of genes involved in phenylpropanoid and flavonoid biosynthesis pathway in different organs (A) and four fruit stages (stage 1-4) (B) of bitter melon plants.The vertical axes show the expression relative to cyclophilin and the height of each bar and the error bars indicate the mean and standard error, respectively, from three independent measurements.Y-leaves: young leave; M-leaves: mature leaves; F-flowers: female flowers; M-flowers: male flowers.Letters a-e indicate significant differences (p < 0.05).
).The expression of phenylalanine ammonia-lyase (McPAL) and dihydroflavonol 4-reductase (McDFR) was the highest at 0 DAS, intermediate at 5 and 15 DAS, and the lowest at 10 DAS.The expression patterns of McC4H, Mc4CL, McCHS, and McF3H were similar, with slightly more expression observable at 5 DAS than at other developmental stages.McCHI showed higher expression levels at 5 and 10 DAS, and lower levels at 0 and 15 DAS.McFLS expression was the highest at 0, 5, and 15 DAS, and the lowest at 10 DAS.The expression level of Mc3GT increased from 0 to 5 to 10 DAS, reaching its highest level at 15 DAS.
The expression of phenylalanine ammonia-lyase (McPAL) and dihydroflavonol 4reductase (McDFR) was the highest at 0 DAS, intermediate at 5 and 15 DAS, and the lowest at 10 DAS.The expression patterns of McC4H, Mc4CL, McCHS, and McF3H were similar, with slightly more expression observable at 5 DAS than at other developmental stages.McCHI showed higher expression levels at 5 and 10 DAS, and lower levels at 0 and 15 DAS.McFLS expression was the highest at 0, 5, and 15 DAS, and the lowest at 10 DAS.The expression level of Mc3GT increased from 0 to 5 to 10 DAS, reaching its highest level at 15 DAS.

Figure 3 .Figure 3 .
Figure 3. Expression levels of phenylpropanoid and flavonoid biosynthetic genes in different developmental stages of bitter melon plantlets.The height of each bar and the error bars indicate the mean and standard error, respectively, from three independent measurements.The vertical axes show Figure 3. Expression levels of phenylpropanoid and flavonoid biosynthetic genes in different developmental stages of bitter melon plantlets.The height of each bar and the error bars indicate the mean and standard error, respectively, from three independent measurements.The vertical axes show the expression relative to cyclophilin.Units on the horizontal axes indicate the days after sowing.Letters a-d indicate significant differences (p < 0.05).
The expression levels of Mc3GT increased from 0 to 15 DAS, corresponding to the rutin accumulation levels.McDFR expression was high at 0 and 5 DAS, but low at 10 and 15 DAS, corresponding to the catechinhydrate levels.Similarly, the expression of McCOMT was found to correspond with ferulic acid content.We found that the expression levels of Mc4CL, McCHS, McCHI, McF3H, and McFLS increased from 0 to 5 DAS, but then decreased at 10 DAS before increasing again at 15 DAS.However, the content of phenylpropanoid and flavonoid did not change correspondingly.These results suggest McPAL, McC4H, Mc3GT, McDFR, and McCOMT might be intimately related to the phenylpropanoid and flavonoid content in bitter melon plantlets.2.5.Effects of White, Blue, and Red Lights on the Expression of Phenylpropanoid and Flavonoid Genes Expression patterns of phenylpropanoid and flavonoid biosynthetic genes in bitter melon were investigated at 7, 14, and 21 days under white, blue, or red LED illumination, using real time-PCR (Figure 4).Under white light, the expression of McC4H, McCOMT, McFLS, McDFR, and Mc3GT increased gradually from 7 to 21 days, whereas that of McPAL and McCHI transcripts reached a peak level at 14 days, and then declined.The expression of Mc4CL and McCHS was recovered at 21 days after a decrease at 14 days.Expression of McF3H did not change significantly under white light illumination.Under red light conditions, the expression of many phenylpropanoid and flavonoid genes, including McPAL, Mc4CL, McCOMT, McCHS, McCHI, McF3H, McFLS, and Mc3GT, gradually decreased from 7 to 21 days, except for McC4H.Similarly, buckwheat seedlings exposed to red light showed a reduced expression of most orthologous genes after two days of irradiation [27].The expression of McDFR recovered at 21 days after a decrease at 14 days under red light illumination.Compared with the effects of white and red light illumination, bitter melon seedlings exposed to blue light showed greater changes in the expression of phenylpropanoid and flavonoid biosynthetic genes.Expression of McPAL, McC4H, Mc4CL, McCHS, McCHI, McF3H, McFLS, McDFR, and Mc3GT was considerably upregulated compared with seedlings grown under white or red light.Under blue light conditions, the expression of McC4H, Mc4CL, McCOMT, McCHS, McF3H, McFLS, McDFR, and Mc3GT was high at seven days, decreased at 14 days, and then increased again at 21 days.Expression of McCHI increased dramatically at 21 days under blue light illumination.However, blue light irradiation had no effect on the expression of McPAL.Expression of McPAL, McC4H, Mc4CL, McCHS, McCHI, McF3H, McFLS, McDFR, and Mc3GT was considerably upregulated compared with seedlings grown under white or red light.Under blue light conditions, the expression of McC4H, Mc4CL, McCOMT, McCHS, McF3H, McFLS, McDFR, and Mc3GT was high at seven days, decreased at 14 days, and then increased again at 21 days.Expression of McCHI increased dramatically at 21 days under blue light illumination.However, blue light irradiation had no effect on the expression of McPAL.

Figure 4 .
Figure 4. Expression levels of phenylpropanoid and flavonoid biosynthetic genes in bitter melon exposed to white, blue, and red LED.The height of each bar and the error bars indicate the mean and standard error, respectively, from three independent measurements.The vertical axes show the expression relative to cyclophilin.Units on the horizontal axes indicate the days after irradiation.Letters a−g indicate significant differences (p < 0.05).

Figure 4 .
Figure 4. Expression levels of phenylpropanoid and flavonoid biosynthetic genes in bitter melon exposed to white, blue, and red LED.The height of each bar and the error bars indicate the mean and standard error, respectively, from three independent measurements.The vertical axes show the expression relative to cyclophilin.Units on the horizontal axes indicate the days after irradiation.Letters a-g indicate significant differences (p < 0.05).

Table 1 .
Comparison of phenylpropanoid and flavonoid biosynthetic genes of bitter melon with the most orthologous genes.

Table 2 .
Accumulation of phenolic compounds in different organs of bitter melon plants (µg/g dry weight).

Table 3 .
Accumulation of phenolic compounds in the four fruit stages of bitter melon (µg/g dry weight).Values are expressed as means ± standard deviation from three independent measurements.Letters a-c indicate significant differences (p < 0.05).

Table 4 .
Accumulation of phenylpropanoid and flavonoid compounds in different developmental stages of bitter melon plantlets (µg/g dry weight).Whole bitter melon plantlets were sampled at 0, 5, 10, and 15 DAS and used for HPLC analysis.Values are expressed as means ± standard deviation from three independent measurements.Letters a-d indicate significant differences (p < 0.05).DAS: days after sowing.

Table 5 .
Caffeic acid, rutin, and kaempferol content in bitter melon with white, blue, and red LED (µg/g dry weight).