Interaction of Arylidenechromanone/Flavanone Derivatives with Biological Macromolecules Studied as Human Serum Albumin Binding, Cytotoxic Effect, Biocompatibility Towards Red Blood Cells

The aim of this study was to determine the cytotoxic effect of 3-arylidenechromanone (1) and 3-arylideneflavanone (2) on HL-60 and NALM-6 cell lines (two human leukemia cell lines) and a WM-115 melanoma cell line. Both compounds exhibited high cytotoxic activity with higher cytotoxicity exerted by compound 2, for which IC50 values below 10 µM were found for each cell line. For compound 1, the IC50 values were higher than 10 µM for HL-60 and WM-115 cell lines, but IC50 < 10 µM was found for the NALM-6 cell line. Both compounds, at the concentrations close to IC50 (concentration range: 5–24 µM/L for compound 1 and 6–10 µM/L for compound 2), are not toxic towards red blood cells. The synthesized compounds were characterized using spectroscopic methods 1H- and 13C-NMR, IR, MS, elemental analysis, and X-ray diffraction. The lipophilicity of both synthesized compounds was determined using an RP-TLC method and the logP values found were compared with the theoretical ones taken from the Molinspiration Cheminformatics (miLogP) software package. The mode of binding of both compounds to human serum albumin was assessed using molecular docking methods.


Introduction
Flavonoids constitute a large group of natural and synthetic polyphenolic compounds with a wide range of antioxidant, anti-allergic, anti-inflammatory, anti-microbial, anti-coagulant, anti-cholesterol, or anti-cancer activities.These properties often depend on unknown structure-related interactions with nucleic acids and proteins [1,2].Due to their antioxidant properties, flavonoids interfere with the initiation, promotion, and progression of cancer.Flavonoids modulate the activities of various enzymes and receptors involved in several signal transduction pathways, thus affecting cell proliferation, apoptosis, as well as the processes of inflammation, angiogenesis, and metastasis [3].Due to their multidirectional mechanisms of action, flavonoids are promising candidates for novel anti-cancer agents and are being extensively investigated for the treatment of neoplastic diseases [3][4][5].For instance, it was found that, in vivo, quercetin exerts a chemo-preventive effect on prostate cancer (reduces the cell viability) by downregulating the activity of pro-proliferative and anti-apoptotic proteins [6].Additionally, synthetic derivatives of naturally occurring flavonoids (e.g., derivatives of chroman-4-one and tiochromane-4-one [7]) are studied for their anti-cancer properties.
3-arylidenechroman-4-ones, a group of naturally occurring homoisoflavones, can be obtained by the condensation of aryl aldehydes with chroman-4-ones (chromanones).It was first synthesized by Robinson and co-workers in the early twenties by condensation of chromanon with the corresponding aryl aldehyde using alcoholic potassium hydroxide as a catalyst [8].Other catalysts have also been proposed.In 1979, Levai and Schag synthesized E-3-arylidenechroman-4-ones using piperidine as a catalyst, while piperidine has also been successfully used as a catalyst for the synthesis of 3-benzylidenechroman-4-ones and 3-benzylideneflavanones [9,10].In recent years, Kupcewicz and co-authors [11], as well as our group [12], have investigated the cytotoxicity of E and Z isomers of 3-arylideneflavanone derivatives.Those compounds exhibit high cytotoxicity with IC 50 < 10 µM.However, no significant difference in cytotoxicity of E and Z isomers was observed.In this study the structure of (E)-3-(4-N,N-diethylaminobenzylidene)chroman-4-one (1) and (E)-3-(4-N,N-diethylaminobenzylidene)-2-phenylchroman-4-one (2) was elucidated using single crystal X-ray diffraction (XRD), MS, and NMR spectroscopy.Despite being first synthesized in the 1970s [9,10], compound 1 was presented as an American patent in 2008 [13] and compound 2 was synthesized in 1993 by Pijewska [14], but their cytotoxic activity has never been studied.The cytotoxicity of 3-arylidenechromanone and flavanone substituted with p-aminodiethyl on C3 atom in benzene ring has also been investigated.In addition, the lipophilicity (miLogP) of 1 and 2 and their activity against red blood cells were determined.The mode of binding to human serum albumin (HSA, a protein important in binding and transport of various drugs [15]) has also been elucidated by molecular docking methods.Computational docking of ligand binding to target proteins is often performed as part of Structure-Based Virtual Screening used in drug discovery and this approach enables molecular interactions with macromolecules to be predicted [16].We also assess the lipophilicity and their influence on the various biological properties.
The ν (C=O) band for compound 1 was observed at 1663 cm −1 , and bands typical for aromatic rings were noted at 1600, 1584, and 1524 cm −1 .An IR spectrum of 2 showed a band at 1654 cm −1 and typical bands for the aromatic rings at 1603, 1559, and 1524 cm −1 .The 1 H-NMR spectrum for compound 1 revealed the presence of a triplet from -CH3 protons at 1.12 ppm and a quartet around 3.42 ppm corresponding to the CH2 protons (see Figure S1).A singlet for =C-H was observed at δ 5.45 ppm.At δ 6.75 ppm, a doublet for the C2-H protons was observed.The characteristic signals δ 7.02-7.85ppm correspond to the aromatic protons.The 1 H-NMR spectrum for compound 2 revealed the presence of a triplet from -CH3 protons at 1.12 ppm (see Figure S2).A quartet corresponding to the CH2 protons was observed at δ 3.36 ppm.At δ 6.70 ppm a singlet for C2-H protons was noted.The signals at δ 6.81-7.79ppm correspond to the aromatic protons.A singlet for =C-H was observed at δ 7.94 ppm.In the 13 C-NMR spectrum for compound 1, the characteristic CH3 group signal was found at δ 12.9 ppm, while the -CH2 group was observed at 44.3 ppm (see Figure S1a).The C2-H carbon signal was observed at δ 78.1 ppm.At δ 181.0 ppm, a signal for C=O was observed.In addition the 13 C-NMR spectrum showed the presence of CHarom and Carom signals, which were expected for the postulated structure of (E)-3-(4-N,N-diethylaminobenzylidene)chroman-4-one.For compound 2, the 13 C-NMR spectrum showed the presence of signals located at δ 12.9 ppm, attributed to a CH3 group and at -CH2 group at δ 44.3 ppm (see Figure S2a).In the 13 C-NMR spectrum, a -C=O related resonance was found at δ 181.2 ppm, those for CHarom and Carom were also found.The mass spectrum revealed a band in 1 at m/z = 308.2,indicating ([M + H] + ), and in 2, at m/z = 384, indicating the presence of an [M + H] ion.
The ν (C=O) band for compound 1 was observed at 1663 cm −1 , and bands typical for aromatic rings were noted at 1600, 1584, and 1524 cm −1 .An IR spectrum of 2 showed a band at 1654 cm −1 and typical bands for the aromatic rings at 1603, 1559, and 1524 cm −1 .The 1 H-NMR spectrum for compound 1 revealed the presence of a triplet from -CH 3 protons at 1.12 ppm and a quartet around 3.42 ppm corresponding to the CH 2 protons (see Figure S1).A singlet for =C-H was observed at δ 5.45 ppm.At δ 6.75 ppm, a doublet for the C2-H protons was observed.The characteristic signals δ 7.02-7.85ppm correspond to the aromatic protons.The 1 H-NMR spectrum for compound 2 revealed the presence of a triplet from -CH 3 protons at 1.12 ppm (see Figure S2).A quartet corresponding to the CH 2 protons was observed at δ 3.36 ppm.At δ 6.70 ppm a singlet for C2-H protons was noted.The signals at δ 6.81-7.79ppm correspond to the aromatic protons.A singlet for =C-H was observed at δ 7.94 ppm.In the 13 C-NMR spectrum for compound 1, the characteristic CH 3 group signal was found at δ 12.9 ppm, while the -CH 2 group was observed at 44.3 ppm (see Figure S1a).The C2-H carbon signal was observed at δ 78.1 ppm.At δ 181.0 ppm, a signal for C=O was observed.In addition the 13 C-NMR spectrum showed the presence of CH arom and C arom signals, which were expected for the postulated structure of (E)-3-(4-N,N-diethylaminobenzylidene)chroman-4-one.For compound 2, the 13 C-NMR spectrum showed the presence of signals located at δ 12.9 ppm, attributed to a CH 3 group and at -CH 2 group at δ 44.3 ppm (see Figure S2a).In the 13 C-NMR spectrum, a -C=O related resonance was found at δ 181.2 ppm, those for CH arom and C arom were also found.The mass spectrum revealed a band in 1 at m/z = 308.2,indicating ([M + H] + ), and in 2, at m/z = 384, indicating the presence of an [M + H] ion.

Cytotoxic Activity
The cytotoxicity of the synthesized compounds was evaluated in vitro by the MTT assay using three human cancer cell lines HL-60, NALM-6, and WM-115.The results, expressed as inhibitory concentration (IC50), are shown in Table 3. Compound 2 exhibited high cytotoxic activity with IC50 = 6.45 ± 0.69 µM, which is nine times lower than for 3-benzylideneflavanone and 154 times lower than for 4-chromanone, the reference compounds used in the WM-115 cell line.For the other cell lines, IC50 values were also satisfactory.In the NALM-6 cell culture, compound 1 showed an IC50 value almost 78 times lower than for 4-chromanone and more than three-fold lower than for 3benzylideneflavanone.Perjesi and coworkers [23] also reported cytotoxicity of a series of 3benzylidene-4-chromanones towards several cell lines.The authors found that most of the compounds inhibited the growth of 50% of HL-60 cells at the concentrations between 3-28 µM with 3-(3-bromobenzylidene)-2,3-dihydro-1-benzopyran-4-one as the most active.In the current study we reveal that both synthesized compounds (1 and 2) exhibit good anti-proliferative properties at low micromolar range which make them promising candidates for further in vitro and in vivo studies.

Cytotoxic Activity
The cytotoxicity of the synthesized compounds was evaluated in vitro by the MTT assay using three human cancer cell lines HL-60, NALM-6, and WM-115.The results, expressed as inhibitory concentration (IC 50 ), are shown in Table 3. Compound 2 exhibited high cytotoxic activity with IC 50 = 6.45 ± 0.69 µM, which is nine times lower than for 3-benzylideneflavanone and 154 times lower than for 4-chromanone, the reference compounds used in the WM-115 cell line.For the other cell lines, IC 50 values were also satisfactory.In the NALM-6 cell culture, compound 1 showed an IC 50 value almost 78 times lower than for 4-chromanone and more than three-fold lower than for 3-benzylideneflavanone.Perjesi and coworkers [23] also reported cytotoxicity of a series of 3-benzylidene-4-chromanones towards several cell lines.The authors found that most of the compounds inhibited the growth of 50% of HL-60 cells at the concentrations between 3-28 µM with 3-(3-bromobenzylidene)-2,3-dihydro-1-benzopyran-4-one as the most active.In the current study we reveal that both synthesized compounds (1 and 2) exhibit good anti-proliferative properties at low micromolar range which make them promising candidates for further in vitro and in vivo studies.[11] 33.3 ± 3.0 29.5 ± 4.7 59.4 ± 0.9

Rbcs Lysis Assay
In studies on the integrity of the erythrocyte membrane, statistically significant results in comparison with saline control were obtained for compound 2 at the highest concentrations tested (9 and 10 µmol/L) (Figure 4), which implies that compounds at these concentrations contributed to red blood cell breakdown.However, the values of hemolysis did not exceed 10%, which is considered to be a clinically important threshold of cytotoxicity [24].
In contrast, compound 1 did not lead to any significant increase in the hemolysis rate, therefore, it might be concluded that both examined compounds do not show adverse effects on the integrity of the red blood cells (RBCs) membrane over the entire concentration range.In studies on the integrity of the erythrocyte membrane, statistically significant results in comparison with saline control were obtained for compound 2 at the highest concentrations tested (9 and 10 µmol/L) (Figure 4), which implies that compounds at these concentrations contributed to red blood cell breakdown.However, the values of hemolysis did not exceed 10%, which is considered to be a clinically important threshold of cytotoxicity [24].
In contrast, compound 1 did not lead to any significant increase in the hemolysis rate, therefore, it might be concluded that both examined compounds do not show adverse effects on the integrity of the red blood cells (RBCs) membrane over the entire concentration range.

RBCs Morphology
When a drug molecule is administered intravenously a series of interactions with blood cells might be initiated.The effects of 1 and 2 on the morphology of erythrocytes were visualized by microscope studies (Figure 5).
Both tested compounds 1 and 2, contributed to the formation of echinocytes.Echinocytes accounted for the major part of erythrocytes when 24 µmol/L compound 1 was applied.As presented in Figure 5, compound 2 led to the formation of echinocytes over the entire concentration range, The results are presented as the percentage of hemolysis obtained from the interaction of studied compounds with 2% red blood cells (RBCs) suspension, compared to the positive control Triton X-100 at 0.2% (100% hemolysis) (mean ± SD; n = 4), * p < 0.05 vs. control.

RBCs Morphology
When a drug molecule is administered intravenously a series of interactions with blood cells might be initiated.The effects of 1 and 2 on the morphology of erythrocytes were visualized by microscope studies (Figure 5).
Both tested compounds 1 and 2, contributed to the formation of echinocytes.Echinocytes accounted for the major part of erythrocytes when 24 µmol/L compound 1 was applied.As presented in Figure 5, compound 2 led to the formation of echinocytes over the entire concentration range, however, the percentage of this erythrocyte form differed according to the concentration of 2. The transformation of biconcave erythrocytes to echinocytes occurs naturally in blood vessels, and could be also seen in control samples.It has been proved that echinocytosis is a reversible transformation caused by various chemical and physical factors including increased ion strength, alkaline pH, and decreased in adenosine triphosphate (ATP) level [25].
Molecules 2018, 23, x 8 of 16 however, the percentage of this erythrocyte form differed according to the concentration of 2. The transformation of biconcave erythrocytes to echinocytes occurs naturally in blood vessels, and could be also seen in control samples.It has been proved that echinocytosis is a reversible transformation caused by various chemical and physical factors including increased ion strength, alkaline pH, and decreased in adenosine triphosphate (ATP) level [25].

Computational Studies
When drugs circulate in blood, they interact with biological macromolecules such as plasma proteins; these albumins constitute the majority and are important in the pharmacokinetics of medicines.For derivatives 1 and 2, tenfold blind experiments of docking to HSA were performed to establish locations of their binding sites and the strength of the hypothetical interactions.The compounds were found to bind through hydrophobic interactions, 1 in four different places (near the cleft site, at the IB and FA1 sites, above the IIA-drug site and some additional atypical place) with average ΔG° −7.5 kcal/mol, whereas 2, only in the IB FA1 site with average ΔG° −9.5 kcal/mol (Figure 6 A,B).This single binding most likely resulted from the presence of an additional phenyl ring at the C2 position.The π…π stacking was an important interaction between phenyl rings of the docked compounds and the aromatic side chains of tyrosine and phenylalanine.In the IB FA1 site, the aromatic rings of Tyr138 and 4-diethyloaminobenzylidene substituent were parallel and were only slightly offset from each other with a distance between 4.4 and 4.5 Å.The phenyl ring in position 2 was located between the phenyl rings of Tyr138 and Phe125.The pyran ring was located between the phenyl ring of Phe125 and the aliphatic chain of Lys137 (Figure 6 C and D).In contrast, compound 1 was located in the IB FA1 site inversely and showed less potent hydrophobic interactions.The rings of the 4-N,N-diethyloaminobenzylidene substituent and Tyr138 were arranged in a parallel orientation, but were much more offset.The pyran ring was perpendicular to the phenyl ring of Tyr140 with a distance greater than 5 Å.His247 and Tyr30 were isolated with the distance greater than 20 Å.For compound 1, mainly hydrophobic interactions with the aliphatic amino acid residues were observed in the other binding sites, and in the case of the cleft site, an oblique interaction

Computational Studies
When drugs circulate in blood, they interact with biological macromolecules such as plasma proteins; these albumins constitute the majority and are important in the pharmacokinetics of medicines.For derivatives 1 and 2, tenfold blind experiments of docking to HSA were performed to establish locations of their binding sites and the strength of the hypothetical interactions.The compounds were found to bind through hydrophobic interactions, 1 in four different places (near the cleft site, at the IB and FA1 sites, above the IIA-drug site and some additional atypical place) with average ∆G • −7.5 kcal/mol, whereas 2, only in the IB FA1 site with average ∆G • −9.5 kcal/mol (Figure 6A,B).This single binding most likely resulted from the presence of an additional phenyl ring at the C2 position.The π . . .π stacking was an important interaction between phenyl rings of the docked compounds and the aromatic side chains of tyrosine and phenylalanine.In the IB FA1 site, the aromatic rings of Tyr138 and 4-diethyloaminobenzylidene substituent were parallel and were only slightly offset from each other with a distance between 4.4 and 4.5 Å.The phenyl ring in position 2 was located between the phenyl rings of Tyr138 and Phe125.The pyran ring was located between the phenyl ring of Phe125 and the aliphatic chain of Lys137 (Figure 6C,D).In contrast, compound 1 was located in the IB FA1 site inversely and showed less potent hydrophobic interactions.
The rings of the 4-N,N-diethyloaminobenzylidene substituent and Tyr138 were arranged in a parallel orientation, but were much more offset.The pyran ring was perpendicular to the phenyl ring of Tyr140 with a distance greater than 5 Å.His247 and Tyr30 were isolated with the distance greater than 20 Å.For compound 1, mainly hydrophobic interactions with the aliphatic amino acid residues were observed in the other binding sites, and in the case of the cleft site, an oblique interaction between the pyran ring with the Tyr452 phenyl ring could be possible within a distance of 3.6 Å.In one atypical place, a similar interaction with the imidazole ring of His146 could take place within a distance of 3.3 Å.No specific hydrogen bonds were detected for either compounds.
To predict other properties of compounds 1 and 2, the Molinspiration web tool was used and the drug descriptors were counted.For the Molinspiration GPCR ligand, Ion channel modulator, Kinase inhibitor, Nuclear receptor ligand, enzyme inhibitor and protease inhibitor bioactivity the scores were < zero or near zero, indicating that the compounds were not typical inhibitors or agonists of the listed types of proteins.Ligand efficiencies (LE) for derivatives calculated from computationally estimated ∆G • and known number of heavy atoms, theoretical logP and logP/LE (LELP), divided by the predicted number of binding sites were different for two compounds, but were still within the range of drug-likeliness (Table 4).
The experimental lipophilicity of the synthesized compounds was determined using RP-TLC: logP values 3.43 was obtained for compound 1 and 5.69 for compound 2. Theoretical values of lipophilicity were calculated using Molinspiration Cheminformatics (miLogP):milogP 4.47 was obtained for 1 and 6.05 for 2, i.e., similar to those obtained experimentally.Greater values of experimental and theoretical lipophilicity were noted for 2 because of the presence of the phenyl ring in the C2 position.Thus, molecular docking and Molinspiration predictions suggest that 1 and 2 have greater hydrophobicity than 1, but also indicate potential specificity influence of an additional phenyl ring.

Materials and Methods
The second compound 2 was synthesized according to Pijewska [14].Compounds 1 and 2 were purified by crystallization from methanol.All solvents (methanol, ethanol, and toluene) used in this work were purchased from Sigma-Aldrich (Sant Louis, MO, USA) and POCH (Gliwice, Poland) chemical companies and were used without further purification.
Melting points were determined on a Büchi Melting Point B-540 apparatus in the capillary mode and they were uncorrected.The infrared transmission spectra of the crystalline products were recorded using a Nexus Thermo Nicolet FT-IR spectrophotometer (Wien, Austria; Faculty of Chemistry, University of Lodz).The MS-ESI were measured on Varian 500, MS LC Ion Trap mass spectrometer (Santa Clara, CA, United States; Faculty of Chemistry, University of Lodz).Elemental analyses were performed in the Faculty of Chemistry (University of Lodz) using a Vario Micro Cube (Langenselbold, Germany) by Elemental analyzer. 1H-and 13 C-NMR spectra were recorded on a Bruker Avance III 600 MHz (Karlsruhe, Germany).Both samples were dissolved in DMSO deuterated.Chemical shifts are given in ppm, coupling constants in Hz.Computational studies were used to predict the properties and future use of potential drugs.Molinspiration Cheminformatics

Materials and Methods
The second compound 2 was synthesized according to Pijewska [14].Compounds 1 and 2 were purified by crystallization from methanol.All solvents (methanol, ethanol, and toluene) used in this work were purchased from Sigma-Aldrich (St. Louis, MO, USA) and POCH (Gliwice, Poland) chemical companies and were used without further purification.
Melting points were determined on a Büchi Melting Point B-540 apparatus in the capillary mode and they were uncorrected.The infrared transmission spectra of the crystalline products were recorded using a Nexus Thermo Nicolet FT-IR spectrophotometer (Wien, Austria; Faculty of Chemistry, University of Lodz).The MS-ESI were measured on Varian 500, MS LC Ion Trap mass spectrometer (Santa Clara, CA, United States; Faculty of Chemistry, University of Lodz).Elemental analyses were performed in the Faculty of Chemistry (University of Lodz) using a Vario Micro Cube (Langenselbold, Germany) by Elemental analyzer. 1H-and 13 C-NMR spectra were recorded on a Bruker Avance III 600 MHz (Karlsruhe, Germany).Both samples were dissolved in DMSO deuterated.Chemical shifts are given in ppm, coupling constants in Hz.Computational studies were used to predict the properties and future use of potential drugs.Molinspiration Cheminformatics (http://www.molinspiration.com/products.html)was used for the calculation of important molecular properties, molecular processing, bioactivity, and the high-quality depiction of new molecules.Molecular docking of the compounds to the HSA crystal structure 1E7I [21] was performed in AutodockVina 1.1.2(http://vina.scripps.edu)[22], as previously described [12].
Lipophilicity was determined using an RP-TLC method with small amounts of organic solvents.Assessment of erythrotoxicity of compounds 1 and 2 was made in Laboratory of Bioanalysis in the Department of Pharmaceutical Chemistry, Drug Analysis, and Radiopharmacy in the Medical University of Lodz.The erythrocyte morphology was evaluated using a phase-contrast microscope Opta-Tech (Warsaw, Poland) using the OptaView 7 programme.Biological material (blood) for research came from the Regional Blood Donation Center in Lodz.

Synthesis of (E)-3-(4-N,N-diethylaminobenzylidene)chroman-4-one (1)
A mechanically stirred mixture of 10 mmol of chroman-4-one, 10 mmol 4-N,N-diethylaminobenzaldehyde and five drops of piperidine was heated at 140 • C in an oil bath for 4 h.After cooling the reaction mixture was left for 24 h at room temperature.Next, methanol was added and the resulting was filtered and crystallized from methanol.Compound 1 was thus obtained as a light orange powder.

Determination of Lipophilicity of Flavone Derivatives Using RP-TLC Method
The RP-TLC experiments were performed on TLC plates (5 × 10 cm) RP-18 F254S (Merck, Darmstadt, Germany).The synthesized compounds were dissolved in N,N-dimethylformamide DMF (2 mg/mL).High purity DMF 99.8% was obtained from Chempur (Piekary Slaskie, Poland).The solutions of each compound in DMF were spotted on plates.The spots were observed under UV light at λ = 254 nm.DMF-water solvent system was used as mobile phase.The composition of the solvent system changed from 50%:50% to 95%:5%.All experiments were performed at room temperature.The LogP parameter was calculated using the equation from the calibration curve.gentamicin (25 µg/mL; KRKA, Slovenia).For melanoma WM-115 cells, Dulbecco's minimal essential medium (DMEM; Invitrogen, Grand Island, NY, USA) 1640 was used.Cells were grown at 37 • C in a humidified atmosphere of 5% CO 2 in air.
For all experiments, the studied compounds were dissolved in DMSO (Sigma-Aldrich, St. Louis, MO, USA) and were further diluted in a culture medium to obtain <0.1 % DMSO concentration.In each experiment control without and with 0.1% DMSO was performed.
The cytotoxicity of both compounds, and the reference compounds (4-chromanone and 3-benzylidene-flavanone) was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma, St. Louis, MO, USA) assay, which measures the activity of cellular dehydrogenases [29].Exponentially growing cells were seeded on 96-well plates (Nunc, Roskilde, Denmark).Subsequently, various concentrations of the studied compounds (freshly prepared in DMSO and diluted with complete culture medium) were added.All compounds were tested for their cytotoxicity at a final concentration of 10 −7 -10 −3 M.After 46 h of incubation with the studied compounds, the cells were treated with the MTT reagent and incubation was continued for another two hours.The MTT formazan crystals were dissolved in 20% SDS (Sodium dodecyl sulphate, Sigma-Aldrich, St. Louis, USA) and 50% DMF (Sigma-Aldrich, St. Louis, MO, USA) at pH 4.7, and the absorbance was read at 570 nm on a multifunctional Victor ELISA-plate reader (Perkin Elmer, Turku, Finland).The IC 50 values, the concentration of the test compound, was required to reduce the cell survival fraction to 50% of the controls, and were calculated from concentration response curves and was used as a measure of the sensitivity of the cells to a given treatment.As a control, cultured cells were grown in the absence of drugs.The data points represent the means of at least five to ten repeats ± S.D (standard deviation).

Red Blood Cells Lysis Assay
The studies on biological material were approved by the Bioethics Committee of the Medical University of Lodz, Poland (RNN/27/18/KE).The blood from healthy donors (Blood Donation Centre in Lodz) was collected in tubes containing a solution of potassium EDTA.RBCs were isolated by centrifugation (3000× g, 10 min) at 20 • C and washed three times with 0.9% saline.The studies were performed on four biological samples obtained from different blood donors.
The effects of (E)-3-(4-N,N-diethylaminobenzylidene)-2-phenylchroman-4-one 1 and (E)-3-(4-N,N-diethylaminobenzylidene)-2-phenylchroman-4-one 2 on RBCs was performed using the method described earlier [30,31].Briefly, 2% RBCs suspension prepared in 0.9% saline was incubated at 37 • C with tested compounds added in a 10 µL volume for one hour.The percentage of solvent (methanol) did not exceed 1% of the final sample volume (1 mL).Afterwards, the samples were centrifuged at 1000× g for 10 min (20 • C), and the absorbance of the supernatant was measured at 550 nm wavelength (spectrophotometer (Cecil CE2021, London, England)).Samples containing saline solution were used to measure spontaneous hemolysis of RBCs, while samples with Triton X-100 ((2.0%v/v); Polish Chemical Reagents) constituted 100% of hemoglobin release.The results are presented as a percentage of released hemoglobin.The examined concentrations of tested compounds were chosen on the basis of obtained IC 50 values determined for three human cancer cell lines: HL-60, NALM-6, and WM-115.

RBCs Morphology
A 2% erythrocyte suspension was incubated at 37 • C for 1 h with various concentrations of tested compounds.The morphology of the RBCs was evaluated using a phase contrast Opta-Tech inverted microscope, at 400 times magnification, equipped with image analysis software (OptaView 7).

Statistical Analysis
Statistical analysis was conducted with a commercially available package (Statistica 12.0, StatSoft).All results are presented as mean ± SD.The normality of the distribution of continuous variables was confirmed with the Shapiro-Wilk test.Paired t-tests were used for intergroup comparisons of normally distributed variables.The results were considered significant at p-values lower than 0.05.

Conclusions
This study presents the synthesis, chemical characterization, and biological activity of 3-benzylidenederivatives of chromanone and flavanone.The structure of synthesized compounds was confirmed using NMR, MS, IR, and single crystal X-ray diffraction (XRD) techniques.The anti-cancer properties of synthesized compounds 1 and 2 were evaluated using HL-60, NALM-6, and WM-115 cancer cell lines.Both compounds exerted a greater cytotoxic effect towards all cell lines than the reference 4-chromanone and 3-benzylideneflavanone.Compound 2 exerted the most profound cytotoxic effect towards WM-115 cell line with IC 50 = 6.45 ± 0.69 µmol/L.Lipophilicity of both compounds was determined using an RP-TLC method.Due to the presence of a phenyl substituent in the C2 position of chromanone, the value of logP for compound 2 was higher than that for 1.Both compounds did not affect the integrity of erythrocyte membrane over the examined concentration range and did not contribute to hemolysis.In addition, tested compounds did not induce the pathological changes in the morphology of erythrocytes since only dyscocytes and echinocytes were recognized.Thus, both compounds at the concentrations close to the determined IC 50 values might be regarded as biocompatible towards red blood cells.Computational studies suggest remarkably different ability of the synthetized compounds to bind with HSA, and different specificity as far as the future use as drugs is considered.Taking into consideration the promising cytotoxicity properties, i.e., IC 50 values in a few micromolar range and being harmless to the red blood cells, the arylidenechromanone/flavanone derivatives can be further evaluated as promising anticancer compounds.Supplementary Materials: Supplementary materials are available online.

Figure 1 .
Figure 1.Molecular structures of 1 and 2, with atom-numbering schemes.The anisotropic displacement parameters are shown at the 30% probability level.Hydrogen atoms are drawn as spheres of arbitrary radius.

Figure 1 .
Figure 1.Molecular structures of 1 and 2, with atom-numbering schemes.The anisotropic displacement parameters are shown at the 30% probability level.Hydrogen atoms are drawn as spheres of arbitrary radius.

Figure 2 .
Figure 2. Chains and layer of (1) supported by C-H…O hydrogen bonds (A) that build a 3-D network of hydrogen bonds (B) and view of equivalent molecules linked by hydrogen bonds (top right).

Figure 3 .
Figure 3. Partial molecular packing of 2 showing hydrogen-bonded dimers generated by C-H…O interaction.

Figure 4 .
Figure 4.The effects of compound 1 and 2 on the integrity of the erythrocyte membrane.The results are presented as the percentage of hemolysis obtained from the interaction of studied compounds with 2% red blood cells (RBCs) suspension, compared to the positive control Triton X-100 at 0.2% (100% hemolysis) (mean ± SD; n = 4), * p < 0.05 vs. control.

Figure 4 .
Figure 4.The effects of compound 1 and 2 on the integrity of the erythrocyte membrane.The results are presented as the percentage of hemolysis obtained from the interaction of studied compounds with 2% red blood cells (RBCs) suspension, compared to the positive control Triton X-100 at 0.2% (100% hemolysis) (mean ± SD; n = 4), * p < 0.05 vs. control.

Figure 5 .
Figure 5.The microscope images of erythrocytes treated with compounds 1 and 2. 2% erythrocyte suspension was treated at 37 °C for 60 min with indicated concentrations of compound 1 and 2. Representative phase-contrast images are shown (magnification of 400 times).Black arrows indicate echinocytes.

Figure 5 .
Figure 5.The microscope images of erythrocytes treated with compounds 1 and 2. 2% erythrocyte suspension was treated at 37 • C for 60 min with indicated concentrations of compound 1 and 2. Representative phase-contrast images are shown (magnification of 400 times).Black arrows indicate echinocytes.

Figure 6 .
Figure 6.Tenfold blind docking of derivative 1 and 2 to HSA.Ribbon-stick models present the docking of derivatives (sticks) I-A, C and II-B, D to 1E7I human albumin structure (ribbon) with binding affinity energy (ΔG°) −7.5 ± 0.2 and −9.1 ± 0.1 (kcal/mol ± SD), respectively.C, D are the zoom of IB FA1 binding site area, distances marked by yellow dashed lines.

Figure 6 .
Figure 6.Tenfold blind docking of derivative 1 and 2 to HSA.Ribbon-stick models present the docking of derivatives (sticks) I-A, C and II-B, D to 1E7I human albumin structure (ribbon) with binding affinity energy (∆G • ) −7.5 ± 0.2 and −9.1 ± 0.1 (kcal/mol ± SD), respectively.C, D are the zoom of IB FA1 binding site area, distances marked by yellow dashed lines.