Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China

Chaenomeles speciosa (Sweet) Nakai is a medicinal plant. Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions. In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity. C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas. Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg) had higher free amino acid contents than those from Chongqing (2286.66 mg/kg). Oleanolic acid and ursolic acid levels were highest in samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively. C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g). Among four production areas, Yunnan represented the highest antioxidant activity and α-glucosidase inhibitory activity. In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant. These results would be helpful for evaluating the quality of C. speciosa in the different production areas.


Introduction
The genus Chaenomeles, belonging to the Rosaceae family, is mainly distributed in Korea, Japan and China. There are five wild Chaenomeles species in China including C. speciosa (Sweet) Nakai, C. thibetica Yü, C. cathayensis (Hemsl.) Schneider, C. sinensis (Thouin) Koehne and C. japonica (Thunb.) Lindl. ex Spach. C. speciosa has traditionally been cultivated in Yunnan, Chongqing, Zhejiang and Anhui provinces and its dried fruits are commonly used in traditional medicine for the treatment of the common cold, asthma, hepatitis and rheumatoid arthritis [1]. The fruits of C. speciosa have

TF, TP and Polysaccharide Contents
The dried fruits of C. speciosa from Yunnan had the highest TF content (47.92 ± 3.79 mg/g), which was about 2-fold higher than the level in plants from Chongqing (22.36 ± 0.53 mg/g) ( Figure 1). TF contents of plants from Zhejiang (26.27 ± 1.34 mg/g) and Chongqing were similar and were lower than that of plants from Anhui (34.89 ± 1.49 mg/g). The dried fruits of C. speciosa from Yunnan had a higher TP content (29.15 ± 0.29 mg/g) than samples from Chongqing (18.19 ± 0.37 mg/g), Zhejiang (18.85 ± 0.24 mg/g) and Anhui (22.27 ± 0.49 mg/g). The TP contents from four production areas were significantly higher than the results (13.58 ± 0.13 mg/g) reported by Li et al. [17]. Gao et al. [13] investigated the effect of the drying methods, including freeze-drying and hot air drying, on the TP content of C. speciosa. It is found that all the fruits of C. speciosa processed by both the drying methods have lower TP content (ranging from 2.14 ± 0.10 to 3.28 ± 0.47 mg/g) than the samples from Chongqing in the work. The dried fruits of C. speciosa from Yunnan (27.60 ± 1.56 mg/g), Zhejiang (24.45 ± 3.70 mg/g) and Anhui (25.26 ± 1.31 mg/g) had similar polysaccharide contents, which were higher than that of plants from Chongqing (19.95 ± 1.88 mg/g). Thus, dried fruits of C. speciosa in Yunnan had the highest TF, TP and polysaccharide levels in four production areas.
Molecules 2018, 23, x 3 of 12 (24.45 ± 3.70 mg/g) and Anhui (25.26 ± 1.31 mg/g) had similar polysaccharide contents, which were higher than that of plants from Chongqing (19.95 ± 1.88 mg/g). Thus, dried fruits of C. speciosa in Yunnan had the highest TF, TP and polysaccharide levels in four production areas. Figure 1. TF, TP and polysaccharide contents of the dried fruits of C. speciosa from four production areas in China. Results are shown as mean ± SD (n = 3). Different letters above each bar indicate significant differences (p < 0.05). TF, total flavonoid; TP, total polyphenol.

Free Amino Acid Content
The total amino acid content in samples from Chongqing (2286.66 mg/kg) was lower than that of samples from Yunnan (3411.60 mg/kg), Anhui (3349.83 mg/kg) and Zhejiang (3226.30 mg/kg) ( Table 1). On the contrary, total essential amino acid content was higher in plants from Chongqing (823.60 mg/kg) than in those from Yunnan (632.91 mg/kg), Zhejiang (602.13 mg/kg) and Anhui (647.96 mg/kg). However, the abundance of specific essential amino acids did not reflect the total amino acid and total essential amino acid contents. That is, threonine (26.58 ± 0.88 mg/kg), valine (51.42 ± 1.97 mg/kg) and phenylalanine (107.75 ± 6.63 mg/kg) levels were higher in C. speciosa from Yunnan than in plants from the other three provinces, whereas the opposite trend was observed for methionine (30.29 ± 4.54 mg/kg), isoleucine (13.78 ± 0.75 mg/kg) and leucine (9.41 ± 1.49 mg/kg). The highest levels of isoleucine (30.43 ± 3.43 mg/kg) were found in samples from Chongqing, whereas leucine (34.23 ± 1.40 mg/kg) and lysine (44.07 ± 2.65 mg/kg) levels were highest in samples from Zhejiang. Methionine content (35.79 ± 0.62 mg/kg) was highest in plants from Anhui. Samples from Chongqing had the lowest threonine (5.94 ± 0.56 mg/kg) and phenylalanine (43.60 ± 2.46 mg/kg) contents, whereas valine (30.40 ± 0.62 mg/kg) and lysine (21.03 ± 1.74 mg/kg) levels were lowest in Zhejiang and Anhui samples, respectively.  Figure 1. TF, TP and polysaccharide contents of the dried fruits of C. speciosa from four production areas in China. Results are shown as mean ± SD (n = 3). Different letters above each bar indicate significant differences (p < 0.05). TF, total flavonoid; TP, total polyphenol.

Free Amino Acid Content
The total amino acid content in samples from Chongqing (2286.66 mg/kg) was lower than that of samples from Yunnan (3411.60 mg/kg), Anhui (3349.83 mg/kg) and Zhejiang (3226.30 mg/kg) ( Table 1). On the contrary, total essential amino acid content was higher in plants from Chongqing (823.60 mg/kg) than in those from Yunnan (632.91 mg/kg), Zhejiang (602.13 mg/kg) and Anhui (647.96 mg/kg). However, the abundance of specific essential amino acids did not reflect the total amino acid and total essential amino acid contents. That is, threonine (26.58 ± 0.88 mg/kg), valine (51.42 ± 1.97 mg/kg) and phenylalanine (107.75 ± 6.63 mg/kg) levels were higher in C. speciosa from Yunnan than in plants from the other three provinces, whereas the opposite trend was observed for methionine (30.29 ± 4.54 mg/kg), isoleucine (13.78 ± 0.75 mg/kg) and leucine (9.41 ± 1.49 mg/kg). The highest levels of isoleucine (30.43 ± 3.43 mg/kg) were found in samples from Chongqing, whereas leucine (34.23 ± 1.40 mg/kg) and lysine (44.07 ± 2.65 mg/kg) levels were highest in samples from Zhejiang. Methionine content (35.79 ± 0.62 mg/kg) was highest in plants from Anhui. Samples from Chongqing had the lowest threonine (5.94 ± 0.56 mg/kg) and phenylalanine (43.60 ± 2.46 mg/kg) contents, whereas valine (30.40 ± 0.62 mg/kg) and lysine (21.03 ± 1.74 mg/kg) levels were lowest in Zhejiang and Anhui samples, respectively. Results are shown as mean ± SD (n = 3). Different letters in each column indicate significant differences (p < 0.05). We identified 19 organic acids in C. speciosa samples from four production areas, which is consistent with results reported in the literature [18] (Table 2). Malic acid constituted the largest fraction of total organic acids in samples from Yunnan (66.77%), Zhejiang (40.58%) and Anhui (63.06%).
Oleic acid content represented about 41.77% of total organic acids in samples from Chongqing. Malic, citric, palmitic, linoleic, oleic and 10-hydroxy-hexadecanoic acids together accounted for over 90% of total organic acids in all samples from four production areas and were therefore the focus of quantitative analysis. Representative chromatograms of organic acids in C. speciosa samples are shown in Figure 2. Oxalic, malic, citric, palmitic, linoleic, oleic and stearic acids were identified by matching the retention times to those of known standards. Linoleic and oleic acids could not be quantified due to their co-elution. Therefore, we compared the contents of the remaining five baseline-separated organic acids in samples from four production areas (Table 3). C. speciosa from Yunnan (3.20 ± 0.05 mg/g) and Anhui (1.33 ± 0.08 mg/g) had the highest and lowest levels of oxalic acid, respectively. Malic acid levels in samples from Yunnan (124.96 ± 7.43 mg/g) and Anhui (139.91 ± 4.24 mg/g) were three times higher than those in plants from Chongqing (34.81 ± 0.35 mg/g) and Zhejiang (32.86 ± 0.05 mg/g). Citric acid content in plants from four production areas varied from 10.26-30.91 mg/g. C. speciosa from Chongqing had a higher palmitic acid content (12.04 ± 0.02 mg/g) than samples from Zhejiang (7.41 ± 0.07 mg/g), Anhui (7.45 ± 0.13 mg/g) and Yunnan (5.78 ± 0.28 mg/g). Stearic acid concentration was about two times higher in plants from Chongqing (2.23 ± 0.08 mg/g) than in those from Yunnan (1.05 ± 0.04 mg/g), Zhejiang (1.16 ± 0.10 mg/g) and Anhui (1.09 ± 0.01 mg/g). Results are shown as mean ± SD (n = 3). Different letters in each column indicate significant differences (p < 0.05).

OA and UA Contents
OA and UA were the important ingredients in C. speciosa. The dried fruits of C. speciosa from Zhejiang had the highest concentration of total triterpenic acids (720.89 μg/g), which was over twice that in samples from Chongqing (331.51 μg/g) ( Table 4). Plants from Zhejiang and Yunnan had the highest OA (555.98 ± 20.88 μg/g) and UA (339.97 ± 11.86 μg/g) levels, respectively. In general, the concentration of UA was higher than that of OA in four production areas [1]. Gao et al. [13] also investigated the effect of the drying methods on OA and UA contents of C. speciosa. The OA and UA contents for all the dried fruits of C. speciosa ranged from 2.37 ± 0.01 to 17.38 ± 0.03 mg/g and 15.16 ± 0.10 to 2.52±0.03 mg/g, respectively. These results were at least four times more than the findings of the present study. Table 4. OA and UA contents in the dried fruits of C. speciosa from four production areas. Results are shown as mean ± SD (n = 3). Different letters in each column indicate significant differences (p < 0.05). OA, oleanolic acid; UA, ursolic acid. Anhui. The following organic acids were detected: 1, oxalic acid; 2, malic acid; 3, citric acid; 4, palmitic acid; 5, linoleic acid; 6, oleic acid; 7, octadecanoic acid.

OA and UA Contents
OA and UA were the important ingredients in C. speciosa. The dried fruits of C. speciosa from Zhejiang had the highest concentration of total triterpenic acids (720.89 µg/g), which was over twice that in samples from Chongqing (331.51 µg/g) ( Table 4). Plants from Zhejiang and Yunnan had the highest OA (555.98 ± 20.88 µg/g) and UA (339.97 ± 11.86 µg/g) levels, respectively. In general, the concentration of UA was higher than that of OA in four production areas [1]. Gao et al. [13] also investigated the effect of the drying methods on OA and UA contents of C. speciosa. The OA and UA contents for all the dried fruits of C. speciosa ranged from 2.37 ± 0.01 to 17.38 ± 0.03 mg/g and 15.16 ± 0.10 to 2.52±0.03 mg/g, respectively. These results were at least four times more than the findings of the present study. Results are shown as mean ± SD (n = 3). Different letters in each column indicate significant differences (p < 0.05). OA, oleanolic acid; UA, ursolic acid.

Antioxidant Activity and α-Glucosidase
Inhibitory Activity of C. speciosa DPPH, ABTS and SOA radical-scavenging assays were adopted for evaluating the antioxidant capacities of the dried fruits of C. speciosa ( Figure 3A) from four production areas. The results showed that C. speciosa from Yunnan had the highest DPPH (273.30 ± 15.59 mg/g), ABTS (114.37 ± 0.31 mg/g) and SOA (52.36 ± 1.51 mg/g) radical-scavenging activities. The DPPH activity for Yunnan samples was 8-to 11-fold higher than that of plants from other production areas. In addition, Zhejiang had the lowest level of DPPH (24.32 ± 2.55 mg/g) radical-scavenging activity, while Chongqing samples represented the lowest levels of ABTS (41.68 ± 4.73 mg/g) and SOA (8.11 ± 0.06 mg/g) radical-scavenging capacities. Samples from Zhejiang (43.57 ± 0.83 mg/g) and Anhui (44.79 ± 1.05 mg/g) had similar SOA activity. All these results demonstrate that the dried fruits of C. speciosa from Yunnan had higher DPPH, ABTS and SOA radical-scavenging capacities than plants from other production areas. In addition, α-glucosidase inhibitory activities of C. speciosa from four production areas were also investigated and their results varied vigorously from each other. As shown in Figure 3B, the value of α-glucosidase inhibitory activity was in the order of Chongqing (56.59 ± 6.50 mg/g) < Zhejiang (86.41 ± 4.57 mg/g) < Anhui (172.62 ± 3.69 mg/g) < Yunnan (207.23 ± 0.26 mg/g). It could be deduced that some environment factors [19], such as sunshine, soil and rainfall in different production areas affect the chemical profiles of C. speciosa, which could further affect the antioxidant and α-glucosidase inhibitory activities of C. speciosa.
DPPH, ABTS and SOA radical-scavenging assays were adopted for evaluating the antioxidant capacities of the dried fruits of C. speciosa ( Figure 3A) from four production areas. The results showed that C. speciosa from Yunnan had the highest DPPH (273.30 ± 15.59 mg/g), ABTS (114.37 ± 0.31 mg/g) and SOA (52.36 ± 1.51 mg/g) radical-scavenging activities. The DPPH activity for Yunnan samples was 8-to 11-fold higher than that of plants from other production areas. In addition, Zhejiang had the lowest level of DPPH (24.32 ± 2.55 mg/g) radical-scavenging activity, while Chongqing samples represented the lowest levels of ABTS (41.68 ± 4.73 mg/g) and SOA (8.11 ± 0.06 mg/g) radical-scavenging capacities. Samples from Zhejiang (43.57 ± 0.83 mg/g) and Anhui (44.79 ± 1.05 mg/g) had similar SOA activity. All these results demonstrate that the dried fruits of C. speciosa from Yunnan had higher DPPH, ABTS and SOA radical-scavenging capacities than plants from other production areas. In addition, α-glucosidase inhibitory activities of C. speciosa from four production areas were also investigated and their results varied vigorously from each other. As shown in Figure 3B, the value of α-glucosidase inhibitory activity was in the order of Chongqing (56.59 ± 6.50 mg/g) < Zhejiang (86.41 ± 4.57 mg/g) < Anhui (172.62 ± 3.69 mg/g) < Yunnan (207.23 ± 0.26 mg/g). It could be deduced that some environment factors [19], such as sunshine, soil and rainfall in different production areas affect the chemical profiles of C. speciosa, which could further affect the antioxidant and α-glucosidase inhibitory activities of C. speciosa.

Correlation Analysis
The correlations of TF, TP, polysaccharide, OA and UA contents with antioxidant activity and α-glucosidase inhibitory activity of C. speciosa were examined (Table 5). TF content (p < 0.01) was positively correlated with DPPH (r = 0.88), ABTS (r = 0.94), SOA (r = 0.74) and α-glucosidase inhibitory (r = 0.97) activities. The same was true for TP with DPPH (r = 0.93, p < 0.01), ABTS (r = 0.97, p < 0.01), SOA (r = 0.66, p < 0.05) radical-scavenging activities but not for α-glucosidase inhibitory activity. These results demonstrated that TF and TP possess strong DPPH and ABTS radical-scavenging capacities. The correlation coefficient of TF (or TP) with SOA was lower than that with DPPH or ABTS. The phenolic group in TF and TP would react more easily with DPPH or ABTS radicals than with SOA radicals. Polysaccharide content was correlated with ABTS (r = 0.59, p < 0.05), SOA (r = 0.81, p < 0.01) radical-scavenging activities and α-glucosidase inhibitory activity (r = 0.68, r < 0.05). The lower correlation coefficients of polysaccharide with ABTS than TF or TP might account for the different reaction rates of alcohol and phenolic groups with ABTS radical. A comparison of the correlation coefficients of polysaccharide and TP (or TF) with SOA suggested that polysaccharide could better inhibit the formation of superoxide radical and exhibit stronger SOA

Correlation Analysis
The correlations of TF, TP, polysaccharide, OA and UA contents with antioxidant activity and α-glucosidase inhibitory activity of C. speciosa were examined (Table 5). TF content (p < 0.01) was positively correlated with DPPH (r = 0.88), ABTS (r = 0.94), SOA (r = 0.74) and α-glucosidase inhibitory (r = 0.97) activities. The same was true for TP with DPPH (r = 0.93, p < 0.01), ABTS (r = 0.97, p < 0.01), SOA (r = 0.66, p < 0.05) radical-scavenging activities but not for α-glucosidase inhibitory activity. These results demonstrated that TF and TP possess strong DPPH and ABTS radical-scavenging capacities. The correlation coefficient of TF (or TP) with SOA was lower than that with DPPH or ABTS. The phenolic group in TF and TP would react more easily with DPPH or ABTS radicals than with SOA radicals. Polysaccharide content was correlated with ABTS (r = 0.59, p < 0.05), SOA (r = 0.81, p < 0.01) radical-scavenging activities and α-glucosidase inhibitory activity (r = 0.68, r < 0.05). The lower correlation coefficients of polysaccharide with ABTS than TF or TP might account for the different reaction rates of alcohol and phenolic groups with ABTS radical. A comparison of the correlation coefficients of polysaccharide and TP (or TF) with SOA suggested that polysaccharide could better inhibit the formation of superoxide radical and exhibit stronger SOA radical-scavenging activity than TP (or TF). Furthermore, in the present study, there was no correlation of OA level with DPPH, ABTS, SOA and α-glucosidase inhibitory activities, whereas UA content was correlated with DPPH (p < 0.05) and ABTS (p < 0.05) activities but not with SOA radical-scavenging and α-glucosidase inhibitory activities [13]. All above results indicated that TF, TP, polysaccharide and UA could be the major active constituents for the antioxidant activity of C. speciosa, while TF and polysaccharide were the main components for α-glucosidase inhibitory activity of the plant.

Plant Materials and Reagents
The fruits of C. speciosa were collected during the mature period from Yunnan, Chongqing, Zhejiang and Anhui provinces. The samples were cut in half vertically and dried under sunlight for 10 days. They were then powdered and stored at 4 • C until use.

Analysis of TF and TP
C. speciosa samples (0.1 g) were extracted twice with 80% ethanol solution (v/v, 10 mL) in an ultrasonic bath at 60 • C for 40 min. The extracts were combined and stored at 4 • C until use.
TF content was measured as previously described [20], with some modifications and with quercetin as the standard. The extraction solution was diluted for 2 times. Then the obtained sample solution (50 µL) and 0.066 mol/L sulfite solution (100 µL) were dispensed into a 96-well plate. After 5 min, 10% AlCl 3 solution (w/v, 15 µL) was added and the mixture was incubated at room temperature for 6 min. A 0.5 mol/L NaOH solution (100 µL) was added to terminate the reaction and the absorbance of the mixture at 510 nm was measured with a microplate reader. TF content is expressed as milligrams of quercetin per gram of dry weight of the plant material.
TP content was determined according to the Folin-Ciocalteu method [21], with some modifications. and 20% Na 2 CO 3 solution (w/v, 25 µL) were added. The plate was maintained in the dark for 30 min and the absorbance was measured at 760 nm with a microplate reader. The results are expressed as milligrams of gallic acid equivalent (mg GAE) per gram (g) of the dry weight of the plant material.

Polysaccharide Analysis
The polysaccharide content was determined according to the Entry-Exit Inspection and Quarantine industry standards of China (SN/T 4260-2015) [22]. Powdered C. speciosa sample (0.2 g) was extracted twice with 80% ethanol/H 2 O solution (v/v, 12.5 mL) in an ultrasonic bath at 80 • C for 30 min. The extracts were combined and the mixture was centrifuged at 4000 rpm for 10 min; the supernatant was discarded. The insoluble material was extracted three times with H 2 O (10 mL) by bath-sonication at 120 W and 80 • C for 30 min. The extracts were combined and the mixture was diluted to 100 mL with H 2 O.
Polysaccharide content was analyzed according to the phenol-sulfuric acid method. Sample solution (1 mL), 5% phenol/H 2 O (w/v, 1 mL) and sulfuric acid (5 mL) were mixed in centrifuge tubes. After 10 min, the centrifuge tubes were incubated in a water bath at 30 • C for 20 min, the reaction solution was removed to 96-well plates and the absorbance was measured at 490 nm with a microplate reader. D-(+)-glucose was used to generate the calibration curve.

Free Amino Acid Analysis
Powdered samples of C. speciosa (3 g) were extracted three times with 0.02 mol/L HCl solution (1.5 mL) in an ultrasonic bath at 30 • C for 5 min. The pooled extracts were allowed to stand in the dark for 2 h before centrifugation at 4000 rpm for 2 min. The supernatant (1 mL) was mixed with 7% sulfosalicylic acid (w/v, 1 mL) solution with shaking for 1 min. The mixture was maintained in the dark for 1 h and centrifuged at 15,000 rpm for 15 min. The supernatant (500 µL) was mixed with 1 mol/L triethylamine/ACN solution (250 µL) and 0.1 mol/L phenylisothiocyanate/ACN solution (250 µL). After 1 h, hexane (2 mL) was added and the mixture was shaken vigorously. The bottom layer was passed through a 0.22-µm filter and stored at 4 • C until use.

Organic Acid Analysis
Organic acid content was estimated based on Liu's method [18] with some modifications. Powdered C. speciosa (0.5 g) sample was extracted twice with 7% sulfuric/methanol (v/v, 10 mL) solution at 0 • C for 30 min. The extracts were combined and the mixture was immersed in a water bath at 60 • C for 24 h and then centrifuged at 3000 rpm. The supernatant was sequentially combined with H 2 O (20 mL) and chloroform (20 mL). After 2 h, sodium sulfate (3 g) was added to the sample solution and the bottom layer was used for analysis.
GC-MS analysis of organic acids was performed with a 5977A mass spectrometer system (Agilent Technologies, Santa Clara, CA, USA) and an HP-5ms ultra-inert column (30 m × 250 µm × 0.25 µm). Helium was used as the carrier gas at a flow rate of 1 mL/min. The temperature program was as follows: the column was held at 220 • C for 1 min before increasing the temperature to 228.5 • C at a rate of 5 • C/min and holding for 5 min. The column temperature was increased to 240 • C at a rate of 5 • C/min and held for 1 min, then increased to 290 • C at a rate of 10 • C/min and held for 3 min. The injection volume was 1 µL.

Analysis of OA and UA Contents
OA and UA were extracted using the same method as TP and TF. OA and UA contents were determined with a UPLC system (Waters, Shanghai, China) equipped with an ultraviolet detector. The analysis was performed according to the Pharmacopoeia of the People's Republic of China [24], with some modifications. A reversed-phase C18 column (2.1 × 100 mm, 1.7-µm C18 silica particles) was used for separation at a temperature of 35 • C. The mobile phase consisted of methanol, H 2 O, acetic acid and triethylamine at a volume ratio of 265:35:0.1:0.05. The flow rate and injection volume were 0.3 mL/min and 5 µL, respectively. Absorbance was measured at 210 nm with a microplate reader.

Antioxidant Activity
The extraction method for assessing the antioxidant activity of C. speciosa was the same as that used for TF and TP extractions. The DPPH radical-scavenging activity was evaluated based on a previous report [25]. The extraction solution was diluted for 10 times. Then the obtained sample solution (50 µL) was mixed with 0.12 mg/mL DPPH/ethanol solution (150 µL) and the reaction was incubated at 37 • C for 30 min in the dark. The absorbance was measured at 517 nm and methanol was used as the positive control. The DPPH radical-scavenging rate was calculated according to the formula A = (A 0 − A i )/A 0 × 100, where A, A 0 and A i represent the scavenging rate and absorbance of blank and sample, respectively.
ABTS free radical-scavenging capacity was determined as previously described [26]. Equal volumes of 1.1 mg/mL ABTS/ethanol (50 µL) and 0.68 mg/mL potassium persulfate aqueous (50 µL) solutions were mixed and allowed to stand overnight in the dark. The resultant ABTS free radical working solution (150 µL) and sample solution (50 µL) were added to a 96-well plate and the reaction was allowed to proceed at room temperature for 30 min. The absorbance of the mixture was measured at 734 nm.
The SOA radical-scavenging effect was determined according to a published method [27]. Briefly, 0.3 mmol/L NBT (50 µL) and 0.936 mmol/L NADH (50 µL) were mixed with the sample solution (50 µL) by shaking for 2 min. The mixture was combined with 0.12 mmol/L PMS (50 µL) solution and then maintained at 26 • C for 5 min. The absorbance was determined at 560 nm.
DPPH, ABTS and SOA radical-scavenging activities are all evaluated with a microplate reader and expressed as milligrams of quercetin equivalents per gram dry weight of the plant material.

α-Glucosidase Inhibitory Activity
The extraction method for evaluating α-glucosidase inhibitory activity of C. speciosa was the same as that used for TF and TP extractions. The α-glucosidase inhibitory activity was determined according to Hattori's method [28] with some modifications. The extraction solution was diluted for 20 times. Then the obtained sample solution (20 µL) and 2.0 mmol/L PNPG (20 µL) were added into the phosphate buffer (50 µL, pH 6.8) in sequence and the mixture was put at 37 • C for 10 min. After addition of 0.9 U/mL enzyme (10 µL), the mixture was further put at 37 • C for 20 min. Finally, 1.0 mol/L Na 2 CO 3 (150 µL) was added to end the reaction. The absorbance was determined at 405 nm with a microplate reader. α-Glucosidase inhibitory activity is expressed as milligrams of quercetin equivalents per gram dry weight of the plant material.

Statistical Analysis
Data are expressed as mean ± standard error of mean of triplicate measurements. Statistical analysis was performed using SAS v.9.2 software (SAS Institute, Cary, NC, USA). Significant differences were determined at p < 0.05 by analysis of variance analysis (Duncan's multiple range test) and the least significant difference method.

Conclusions
In this study, we compared the chemical composition, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from four production areas (Yunnan, Chongqing, Zhejiang and Anhui provinces) and examined the relationship of the chemical composition of the plant with antioxidant activity and α-glucosidase inhibitory activity. The results show that C. speciosa has rich chemical composition, good antioxidant activity and α-glucosidase inhibitory activity and C. speciosa from different production areas exhibit variations in chemical composition, possibly due to factors such as sunlight, soil, rainfall and agricultural operations. The abundant light and heat resources in Yunnan might contribute to the high contents of TF, TP and polysaccharides. A correlation analysis indicated that TF, TP, polysaccharides and UA were the major components responsible for the antioxidant activity of C. speciosa, while TF and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant. The results reveal that C. speciosa could be a potential source of natural antioxidants and hypoglycemics in the food industry. The study would be helpful for evaluating the quality of C. speciosa in the different production areas.