Effects on Rotational Dynamics of Azo and Hydrazodicarboxamide-Based Rotaxanes †

The synthesis of novel hydrogen-bonded [2]rotaxanes having two pyridine rings in the macrocycle and azo- and hydrazodicarboxamide-based templates decorated with four cyclohexyl groups is described. The different affinity of the binding sites for the benzylic amide macrocycle and the formation of programmed non-covalent interactions between the interlocked components have an important effect on the dynamic behavior of these compounds. Having this in mind, the chemical interconversion between the azo and hydrazo forms of the [2]rotaxane was investigated to provide a chemically-driven interlocked system enable to switch its circumrotation rate as a function of the oxidation level of the binding site. Different structural modifications were carried out to further functionalize the nitrogen of the pyridine rings, including oxidation, alkylation or protonation reactions, affording interlocked azo-derivatives whose rotation dynamics were also analyzed.


Introduction
Mechanically interlocked compounds are chemical entities composed by, at least two or more entwined components [1][2][3]. The lacking of a covalent bond between the subunits of these compounds allows relative large amplitude motions, such as translation, rotation (pirouetting) or rocking ( Figure 1) [4,5]. During the last decades, the control of the internal dynamics of these species under different external stimuli allowed the development of smart interlocked devices for a broad range of applications [6][7][8][9][10][11][12].

Introduction
Mechanically interlocked compounds are chemical entities composed by, at least two or more entwined components [1][2][3]. The lacking of a covalent bond between the subunits of these compounds allows relative large amplitude motions, such as translation, rotation (pirouetting) or rocking ( Figure 1) [4,5]. During the last decades, the control of the internal dynamics of these species under different external stimuli allowed the development of smart interlocked devices for a broad range of applications [6][7][8][9][10][11][12]. The synthesis of hydrogen-bonded rotaxanes requires the presence of suitable binding sites able to template the formation of the corresponding tetralactam macrocycle. In this sense, the employment of 1,4-dicarboxamides [13][14][15], nitrones [16,17], squaraines [18,19] or di(acylamino)pyrididines The synthesis of hydrogen-bonded rotaxanes requires the presence of suitable binding sites able to template the formation of the corresponding tetralactam macrocycle. In this sense, the employment of 1,4-dicarboxamides [13][14][15], nitrones [16,17], squaraines [18,19] or di(acylamino)pyrididines [20][21][22][23], together with hydrogen-bonding templation with anions [24,25] can be highlighted. In this arena, some of us reported that azodicarboxamides are able to act as effective templates for driving the assembly of hydrogen-bond-assembled [2]rotaxanes [26][27][28]. Moreover, these binding sites can be efficiently interconverted with their reduced hydrazo forms through hydrogenation-dehydrogenation of its nitrogen-nitrogen bond. This type of chemical control was efficiently employed for building stimuli-responsive molecular shuttles [26]. In a different work, the rotation dynamics of these particular systems were shown to depend on the oxidation level of the nitrogen-based binding site [27].
In polyamide-based rotaxanes, the thread-macrocycle affinity can be modulated by the variation of the substituents on the macrocyclic counterpart. Indeed, the presence of electron-donor or electron-withdrawing groups can strengthen or weaken this interaction, breaking or accelerating the rotational motion of the ring [29,30]. In pyridine-containing macrocycles a chemical variation of the pyridyl nitrogen can also undergo changes on the rotational speed [31].
Herein we describe the effect of integrating dicyclohexylmethyl stoppers on an azodicarboxamide template in the preparation of a hydrogen-bond-assembled [2]rotaxane having two pyridine rings into the macrocycle and its interconversion into its corresponding hydrazo surrogate. In addition, some chemical modifications of the pyridine moiety were also achieved, including protonation, oxidation and alkylation reactions for providing a novel set of azodicarboxamide-based rotaxanes. Finally the rotational dynamics of each interlocked molecule was studied by temperature-dependent NMR experiments.

Synthesis of Threads [2H]-2 and 2
The reaction of diphenyl hydrazodicarboxylate (1) with bis(cyclohexylmethyl) amine in the presence of two equivalents of triethylamine provided the hydrazodicarboxamide [2H]-2 in a 51% yield. Next, the oxidation of the hydrazo compound [2H]-2 was carried out using N-bromosuccinimide in the presence of pyridine. This reaction was monitored by IR spectroscopy observing the vanishing of the band ascribed to the NH at 3397 cm −1 of the hydrazo compound and the shifting of the absorption frequency of the CO band from 1653 cm −1 to 1699 cm −1 . After the work-up, the azodicarboxamide 2 was isolated in a 97% yield (Scheme 1).  [20][21][22][23], together with hydrogen-bonding templation with anions [24,25] can be highlighted. In this arena, some of us reported that azodicarboxamides are able to act as effective templates for driving the assembly of hydrogen-bond-assembled [2]rotaxanes [26][27][28]. Moreover, these binding sites can be efficiently interconverted with their reduced hydrazo forms through hydrogenationdehydrogenation of its nitrogen-nitrogen bond. This type of chemical control was efficiently employed for building stimuli-responsive molecular shuttles [26]. In a different work, the rotation dynamics of these particular systems were shown to depend on the oxidation level of the nitrogenbased binding site [27].
In polyamide-based rotaxanes, the thread-macrocycle affinity can be modulated by the variation of the substituents on the macrocyclic counterpart. Indeed, the presence of electron-donor or electronwithdrawing groups can strengthen or weaken this interaction, breaking or accelerating the rotational motion of the ring [29,30]. In pyridine-containing macrocycles a chemical variation of the pyridyl nitrogen can also undergo changes on the rotational speed [31].
Herein we describe the effect of integrating dicyclohexylmethyl stoppers on an azodicarboxamide template in the preparation of a hydrogen-bond-assembled [2]rotaxane having two pyridine rings into the macrocycle and its interconversion into its corresponding hydrazo surrogate. In addition, some chemical modifications of the pyridine moiety were also achieved, including protonation, oxidation and alkylation reactions for providing a novel set of azodicarboxamide-based rotaxanes. Finally the rotational dynamics of each interlocked molecule was studied by temperature-dependent NMR experiments.

Synthesis of Threads [2H]-2 and 2
The reaction of diphenyl hydrazodicarboxylate (1) with bis(cyclohexylmethyl) amine in the presence of two equivalents of triethylamine provided the hydrazodicarboxamide [2H]-2 in a 51% yield. Next, the oxidation of the hydrazo compound [2H]-2 was carried out using Nbromosuccinimide in the presence of pyridine. This reaction was monitored by IR spectroscopy observing the vanishing of the band ascribed to the NH at 3397 cm −1 of the hydrazo compound and the shifting of the absorption frequency of the CO band from 1653 cm −1 to 1699 cm −1 . After the workup, the azodicarboxamide 2 was isolated in a 97% yield (Scheme 1).

Synthesis of Rotaxanes [2H]-3 and 3
The five-component clipping reaction between p-xylylenediamine, 3,5-pyridinedicarbonyl dichloride, the hydrazo template [2H]-2 and triethylamine provided the hydrazo [2]rotaxane [2H]-3 in only 4% yield (Scheme 2). The rotaxane formation reaction using the template 2 leads to the interlocked azo compound 3 in a more reasonable 10% yield thus probing the better templating capability of the azo compound for driving the construction of the tetralactam ring around the thread (Scheme 2).

Synthesis of Rotaxanes [2H]-3 and 3
The five-component clipping reaction between p-xylylenediamine, 3,5-pyridinedicarbonyl dichloride, the hydrazo template [2H]-2 and triethylamine provided the hydrazo [2]rotaxane [2H]-3 in only 4% yield (Scheme 2). The rotaxane formation reaction using the template 2 leads to the interlocked azo compound 3 in a more reasonable 10% yield thus probing the better templating capability of the azo compound for driving the construction of the tetralactam ring around the thread (Scheme 2). These results are in line with those obtained for the assembly of N,N,N′,N′-tetrabenzylhydrazodicarboxamide and its corresponding azo derivative [26]. The hydrazodicarboxamide threads likely adopt a ground state conformation in which the carbonyl hydrogen bond acceptors do not have the ideal orientation of an efficient template [32]. However, the incorporation of two pyridine rings into the tetralactam ring also provokes a noticeable decrease of the yield probably due to a non-ideal aggregation between the thread and the intermediate polyamides, precursors of the interlocked tetralactam [29]. This patent difference is connected with the rotational motion of the ring of single binding site amidebased [2]rotaxanes [16,17,[29][30][31][33][34][35][36][37] which occurs at a higher spinning rate in [2H]-3 than in 3. As usually occurred in polyamide-based rotaxanes, peaks corresponding to the hydrogens of the thread are shifted to lower ppm values due to the presence of the macrocycle around it. Interestingly, the set of signals referred to the hydrogens Hb and Hb' in thread 2, which indicates a slow rotation motion of the amide CO-N bond, emerges in only one signal in 3 after rotaxane formation. Moreover, the substantial upfield shift (Δδ ≈ 0.5 ppm) corresponding to the signal of the methylenic Hd' protons of one of the cyclohexane rings of 3 ( Figure 2d) with respect to the naked thread ( Figure 2c) reveals the establishment of stabilizing CH···π interactions [38,39] which further decelerated the rotational motion [29]. In contrast a variation of the chemical shift in about 0.25 ppm was observed when  These results are in line with those obtained for the assembly of N,N,N ,N -tetrabenzylhydrazodicarboxamide and its corresponding azo derivative [26]. The hydrazodicarboxamide threads likely adopt a ground state conformation in which the carbonyl hydrogen bond acceptors do not have the ideal orientation of an efficient template [32]. However, the incorporation of two pyridine rings into the tetralactam ring also provokes a noticeable decrease of the yield probably due to a non-ideal aggregation between the thread and the intermediate polyamides, precursors of the interlocked tetralactam [29]. 3 Hz) at 4.96 ppm and the peak for the axial one appears as a doublet (J (H1,H2) = 14.1 Hz) at 4.06 ppm. This patent difference is connected with the rotational motion of the ring of single binding site amide-based [2]rotaxanes [16,17,[29][30][31][33][34][35][36][37] which occurs at a higher spinning rate in [2H]-3 than in 3. As usually occurred in polyamide-based rotaxanes, peaks corresponding to the hydrogens of the thread are shifted to lower ppm values due to the presence of the macrocycle around it. Interestingly, the set of signals referred to the hydrogens H b and H b ' in thread 2, which indicates a slow rotation motion of the amide CO-N bond, emerges in only one signal in 3 after rotaxane formation. Moreover, the substantial upfield shift (∆δ ≈ 0.5 ppm) corresponding to the signal of the methylenic H d ' protons of one of the cyclohexane rings of 3 ( Figure 2d) with respect to the naked thread ( Figure 2c) reveals the establishment of stabilizing CH···π interactions [38,39] which further decelerated the rotational motion [29]. In contrast a variation of the chemical shift in about 0.25 ppm was observed when

Chemical Interconversion between Rotaxanes 3
Taking into account the above considerations on the spinning rate of the macrocycle of the interlocked systems, most probably we could switch between two different dynamic states just by exchanging the oxidation state of the dinitrogenated binding site of these rotaxanes (for related compounds, see reference [27]). This is why we assayed the chemical interconversion between the two accessible oxidation states of [2]rotaxanes 3. A reduction of the azo rotaxane 3 with hydrazine quantitatively afforded the [2]rotaxane [2H]-3 (Scheme 3). In the reverse sense, [2H]-3 was completely transformed by oxidation with N-bromosuccinimide in the presence of pyridine. In this way, we switched from a fast ring rotation state of a rotaxane to a slow ring rotation one, and viceversa, by simple and efficient chemical modifications.

Chemical Functionalization of the Pyridine Rings of the Macrocycle of Rotaxane 3
We next evaluated the changes on the pirouetting motion of the macrocycle following some modifications at the pyridinic nitrogen atom. We envisaged that this could modify the affinity of the macrocycle for the azo-based binding site and, consequently, its rotational dynamics. Following this idea we synthesized the azo-derived rotaxane 4 from compound 3, in which the two nitrogen atoms of the pyridine rings were chemically converted into N-oxides by reaction with m-chloroperbenzoic acid (m-CPBA) (Scheme 4). The reaction proceeded smoothly at room temperature obtaining a high yield of the corresponding N-oxide derivative 4.

Chemical Interconversion between Rotaxanes 3
Taking into account the above considerations on the spinning rate of the macrocycle of the interlocked systems, most probably we could switch between two different dynamic states just by exchanging the oxidation state of the dinitrogenated binding site of these rotaxanes (for related compounds, see reference [27]). This is why we assayed the chemical interconversion between the two accessible oxidation states of [2]

Chemical Interconversion between Rotaxanes 3
Taking into account the above considerations on the spinning rate of the macrocycle of the interlocked systems, most probably we could switch between two different dynamic states just by exchanging the oxidation state of the dinitrogenated binding site of these rotaxanes (for related compounds, see reference [27]). This is why we assayed the chemical interconversion between the two accessible oxidation states of [2]rotaxanes 3. A reduction of the azo rotaxane 3 with hydrazine quantitatively afforded the [2]rotaxane [2H]-3 (Scheme 3). In the reverse sense, [2H]-3 was completely transformed by oxidation with N-bromosuccinimide in the presence of pyridine. In this way, we switched from a fast ring rotation state of a rotaxane to a slow ring rotation one, and viceversa, by simple and efficient chemical modifications.

Chemical Functionalization of the Pyridine Rings of the Macrocycle of Rotaxane 3
We next evaluated the changes on the pirouetting motion of the macrocycle following some modifications at the pyridinic nitrogen atom. We envisaged that this could modify the affinity of the macrocycle for the azo-based binding site and, consequently, its rotational dynamics. Following this idea we synthesized the azo-derived rotaxane 4 from compound 3, in which the two nitrogen atoms of the pyridine rings were chemically converted into N-oxides by reaction with m-chloroperbenzoic acid (m-CPBA) (Scheme 4). The reaction proceeded smoothly at room temperature obtaining a high yield of the corresponding N-oxide derivative 4.

Chemical Functionalization of the Pyridine Rings of the Macrocycle of Rotaxane 3
We next evaluated the changes on the pirouetting motion of the macrocycle following some modifications at the pyridinic nitrogen atom. We envisaged that this could modify the affinity of the macrocycle for the azo-based binding site and, consequently, its rotational dynamics. Following this idea we synthesized the azo-derived rotaxane 4 from compound 3, in which the two nitrogen atoms of the pyridine rings were chemically converted into N-oxides by reaction with m-chloroperbenzoic acid (m-CPBA) (Scheme 4). The reaction proceeded smoothly at room temperature obtaining a high yield of the corresponding N-oxide derivative 4. Due to the inherent basicity of the pyridine moiety, the corresponding pyridinium trifluoacetate salt 5 can be easily prepared by treatment of rotaxane 3 with an excess of trifluoacetic acid (Scheme 5). We expected that the double protonation of the tetralactam macrocycle can induce an acceleration of the pirouetting motion around the thread as happened in other fumaramide-based rotaxanes [31]. The corresponding salt 5 was obtained in quantitative yield after complete evaporation of the excess of trifluoroacetic acid under high vacuum. Additionally, the initial compound 3 was easily recovered by deprotonation with the weakly basic resin Amberlyst ® A21. Another modification that can be achieved over the pyridine moiety is the N-alkylation with different aliphatic electrophiles, such as alkyl halides. Indeed, the reaction between rotaxane 3 and allyl iodide provided the corresponding rotaxane 6 in high yield, which was insoluble in chlorinated solvents (Scheme 6). A counteranion exchanged (SbF6 − and PF6 − ) was tested to improve the solubility of the system, but these attempts were fruitless. Due to the inherent basicity of the pyridine moiety, the corresponding pyridinium trifluoacetate salt 5 can be easily prepared by treatment of rotaxane 3 with an excess of trifluoacetic acid (Scheme 5). We expected that the double protonation of the tetralactam macrocycle can induce an acceleration of the pirouetting motion around the thread as happened in other fumaramide-based rotaxanes [31]. The corresponding salt 5 was obtained in quantitative yield after complete evaporation of the excess of trifluoroacetic acid under high vacuum. Additionally, the initial compound 3 was easily recovered by deprotonation with the weakly basic resin Amberlyst ® A21. Due to the inherent basicity of the pyridine moiety, the corresponding pyridinium trifluoacetate salt 5 can be easily prepared by treatment of rotaxane 3 with an excess of trifluoacetic acid (Scheme 5). We expected that the double protonation of the tetralactam macrocycle can induce an acceleration of the pirouetting motion around the thread as happened in other fumaramide-based rotaxanes [31]. The corresponding salt 5 was obtained in quantitative yield after complete evaporation of the excess of trifluoroacetic acid under high vacuum. Additionally, the initial compound 3 was easily recovered by deprotonation with the weakly basic resin Amberlyst ® A21. Another modification that can be achieved over the pyridine moiety is the N-alkylation with different aliphatic electrophiles, such as alkyl halides. Indeed, the reaction between rotaxane 3 and allyl iodide provided the corresponding rotaxane 6 in high yield, which was insoluble in chlorinated solvents (Scheme 6). A counteranion exchanged (SbF6 − and PF6 − ) was tested to improve the solubility of the system, but these attempts were fruitless. Another modification that can be achieved over the pyridine moiety is the N-alkylation with different aliphatic electrophiles, such as alkyl halides. Indeed, the reaction between rotaxane 3 and allyl iodide provided the corresponding rotaxane 6 in high yield, which was insoluble in chlorinated solvents (Scheme 6). A counteranion exchanged (SbF 6 − and PF 6 − ) was tested to improve the solubility of the system, but these attempts were fruitless.

Variable-Temperature 1 H-NMR Experiments: Calculation of the Rotational Barrier
The estimation of the ring affinity towards the binding sites in interlocked molecular shuttles is crucial for a correct control of their internal translational motion. In benzylic amide [2]rotaxanes, the energy barrier for the rotation of the macrocycle around the template is directly correlated to their affinity [16,[29][30][31][33][34][35]. Having this in mind, we decided to calculate the energy barrier of rotation of the pyridine-based tetralactam ring in the synthesized rotaxanes 3 and [2H]-3, and in their corresponding derivatives 4-5 by variable-temperature (VT) NMR experiments (see Supporting Information). Note that if the interactions between the macrocycle and thread are weak enough a fast exchange between the methylene protons (HE) of the macrocycle should be observed. The rate of this process would decrease at lower temperatures, resulting in the splitting of the singlet corresponding to the hydrogens located in axial (HEax) and equatorial (HE'eq) positions in the 1 H-NMR spectrum.
The rate of rotation of [2H]-3 is higher than the one of the azo-derivative 3, indicating a lower level of interaction between the reduced thread and the macrocycle, and thus a faster ring rotation ( Table 1, entries 1-2). This is fully consistent with the reported data in related azo-and hydrazo-based rotaxanes [26,28]. Thus, the energy barrier in [2H]-3 (10.7 kcal·mol −1 ) is very similar to the calculated one in the previously reported rotaxane [2H]-7 (10.4 kcal·mol −1 ), bearing four tetrabenzyl groups as stoppers and the classic macrocyclic component (Figure 3) ( Table 1, entries 1 and 5). On the other hand, when comparing the energy barrier values of rotaxanes 3 (19.1 kcal·mol −1 ) and 7 (14.8 kcal·mol −1 ) a huge effect on the dynamics is observed due mainly to the presence of different stoppers ( Table 1, entries 2 and 6). The higher energy barrier in rotaxane 3 can be explained by the establishment of cooperative CH···π interactions between the macrocycle and the cyclohexyl stoppers [29], in combination with the increase of the steric hindrance of the non-planar cyclohexyl groups compared with the planar phenyl rings, which slow down the spinning rate of the macrocycle. It should be note that dissimilar ring affinity of the azo and hydrazo binding sites in the rotaxane 3 and [2H]-3 (8.4 kcal·mol −1 ) is one of the highest energy gaps reported for switchable binding sites. The oxidation of the pyridine rings to the corresponding N-oxide makes the macrocycle in compound 4 to rotate slightly faster when compared with the ring of the starting azo-based rotaxane 3 (Table 1, entries 2-3). Whereas the macrocycle in the trifluoroacetate salt 5 rotates at nearly the same rate than in the neutral compound, in contrast with the reported data in related hydrogen-bonded rotaxanes [31]. Unfortunately, in the case of the allyl-dipyridinium derivative 6 we were not able to perform VT NMR experiments due to its poor solubility in chlorinated solvents (CDCl3 or C2D2Cl4).

Variable-Temperature 1 H-NMR Experiments: Calculation of the Rotational Barrier
The estimation of the ring affinity towards the binding sites in interlocked molecular shuttles is crucial for a correct control of their internal translational motion. In benzylic amide [2]rotaxanes, the energy barrier for the rotation of the macrocycle around the template is directly correlated to their affinity [16,[29][30][31][33][34][35]. Having this in mind, we decided to calculate the energy barrier of rotation of the pyridine-based tetralactam ring in the synthesized rotaxanes 3 and [2H]-3, and in their corresponding derivatives 4-5 by variable-temperature (VT) NMR experiments (see Supporting Information). Note that if the interactions between the macrocycle and thread are weak enough a fast exchange between the methylene protons (H E ) of the macrocycle should be observed. The rate of this process would decrease at lower temperatures, resulting in the splitting of the singlet corresponding to the hydrogens located in axial (H Eax ) and equatorial (H E'eq ) positions in the 1 H-NMR spectrum.
The rate of rotation of [2H]-3 is higher than the one of the azo-derivative 3, indicating a lower level of interaction between the reduced thread and the macrocycle, and thus a faster ring rotation ( Table 1, entries 1-2). This is fully consistent with the reported data in related azo-and hydrazo-based rotaxanes [26,28]. Thus, the energy barrier in [2H]-3 (10.7 kcal·mol −1 ) is very similar to the calculated one in the previously reported rotaxane [2H]-7 (10.4 kcal·mol −1 ), bearing four tetrabenzyl groups as stoppers and the classic macrocyclic component (Figure 3) ( Table 1, entries 1 and 5). On the other hand, when comparing the energy barrier values of rotaxanes 3 (19.1 kcal·mol −1 ) and 7 (14.8 kcal·mol −1 ) a huge effect on the dynamics is observed due mainly to the presence of different stoppers (Table 1, entries 2 and 6). The higher energy barrier in rotaxane 3 can be explained by the establishment of cooperative CH···π interactions between the macrocycle and the cyclohexyl stoppers [29], in combination with the increase of the steric hindrance of the non-planar cyclohexyl groups compared with the planar phenyl rings, which slow down the spinning rate of the macrocycle. It should be note that dissimilar ring affinity of the azo and hydrazo binding sites in the rotaxane 3 and [2H]-3 (8.4 kcal·mol −1 ) is one of the highest energy gaps reported for switchable binding sites. The oxidation of the pyridine rings to the corresponding N-oxide makes the macrocycle in compound 4 to rotate slightly faster when compared with the ring of the starting azo-based rotaxane 3 (Table 1, entries 2-3). Whereas the macrocycle in the trifluoroacetate salt 5 rotates at nearly the same rate than in the neutral compound, in contrast with the reported data in related hydrogen-bonded rotaxanes [31]. Unfortunately, in the case of the allyl-dipyridinium derivative 6 we were not able to perform VT NMR experiments due to its poor solubility in chlorinated solvents (CDCl 3 or C 2 D 2 Cl 4 ). Table 1. Kinetic and thermodynamic parameters for macrocycle pirouetting obtained from variable-temperature 1 H NMR spectra of the [2]rotaxanes in deuterated solvents. ∆ν is the separation, in Hz, between the resonance peaks of axial (H Eax ) and equatorial (H E'eq ) benzylic hydrogens at low temperature, k c is the rate exchange in s −1 and T c is the coalescence temperature in K. ∆G = is the energy barrier (free energy of activation) of the ring rotation process (see Supplementary Materials).  Table 1. Kinetic and thermodynamic parameters for macrocycle pirouetting obtained from variabletemperature 1 H NMR spectra of the [2]rotaxanes in deuterated solvents. Δν is the separation, in Hz, between the resonance peaks of axial (HEax) and equatorial (HE'eq) benzylic hydrogens at low temperature, kc is the rate exchange in s −1 and Tc is the coalescence temperature in K. ΔG ≠ is the energy barrier (free energy of activation) of the ring rotation process (see Supplementary Materials).

General Information
Unless stated otherwise, all reagents were purchased from Aldrich Chemicals (St. Louis, MO, USA) and used without further purification. HPLC grade solvents (Scharlab, Barcelona, Spain) were nitrogen saturated and were dried and deoxygenated using a Pure-Solv 400 Solvent Purification System (Innovative Technology Inc., Amesbury, MA, USA). Column chromatography was carried out using silica gel (60 Å, 70-200 μm, SDS) as stationary phase, and TLC was performed on precoated silica gel on aluminun cards (0.25 mm thick, with fluorescent indicator 254 nm, Fluka Chemie AG, Buchs, Switzerland) and observed under UV light. All melting points were determined on a Kofler hot-plate melting point apparatus and are uncorrected. 1 H-, 19 F-and 13 C-NMR spectra were recorded at 298 K on Avance 300 and 400 MHz instruments (Bruker, Karlsruhe, Germany). 1 H-NMR chemical shifts are reported relative to Me4Si and were referenced via residual proton resonances of the corresponding deuterated solvent whereas 13 C-NMR spectra are reported relative to Me4Si using the carbon signals of the deuterated solvent. Signals in the 1 H and 13 C NMR spectra of the synthesized compounds were assigned with the aid of DEPT, APT, or two-dimensional NMR experiments (COSY, HMQC and HMBC). Abbreviations of coupling patterns are as follows: br, broad; s, singlet; d, doublet; t, triplet; q, quadruplet; m, multiplet. Coupling constants (J) are expressed in Hz. Highresolution mass spectra (HRMS) were obtained using a time-of-flight (TOF) instrument (Agilent 6220, Agilent Technologies Inc., Santa Clara, CA, USA) equipped with electrospray ionization (ESI). IR spectra were registered on a Nicolet Avatar 360 FTIR spectrophotometer (Thermo Electron Corporation, Madison, WI, USA).

General Information
Unless stated otherwise, all reagents were purchased from Aldrich Chemicals (St. Louis, MO, USA) and used without further purification. HPLC grade solvents (Scharlab, Barcelona, Spain) were nitrogen saturated and were dried and deoxygenated using a Pure-Solv 400 Solvent Purification System (Innovative Technology Inc., Amesbury, MA, USA). Column chromatography was carried out using silica gel (60 Å, 70-200 µm, SDS) as stationary phase, and TLC was performed on precoated silica gel on aluminun cards (0.25 mm thick, with fluorescent indicator 254 nm, Fluka Chemie AG, Buchs, Switzerland) and observed under UV light. All melting points were determined on a Kofler hot-plate melting point apparatus and are uncorrected. 1 H-, 19 F-and 13 C-NMR spectra were recorded at 298 K on Avance 300 and 400 MHz instruments (Bruker, Karlsruhe, Germany). 1 H-NMR chemical shifts are reported relative to Me 4 Si and were referenced via residual proton resonances of the corresponding deuterated solvent whereas 13 C-NMR spectra are reported relative to Me 4 Si using the carbon signals of the deuterated solvent. Signals in the 1 H and 13 C NMR spectra of the synthesized compounds were assigned with the aid of DEPT, APT, or two-dimensional NMR experiments (COSY, HMQC and HMBC). Abbreviations of coupling patterns are as follows: br, broad; s, singlet; d, doublet; t, triplet; q, quadruplet; m, multiplet. Coupling constants (J) are expressed in Hz. High-resolution mass spectra (HRMS) were obtained using a time-of-flight (TOF) instrument (Agilent 6220, Agilent Technologies Inc., Santa Clara, CA, USA) equipped with electrospray ionization (ESI). IR spectra were registered on a Nicolet Avatar 360 FTIR spectrophotometer (Thermo Electron Corporation, Madison, WI, USA). To a solution of diphenyl hydrazodicarboxylate (1) (1.65 g, 6.06 mmol) in chloroform (50 mL) was added bis(cyclohexylmethyl)amine (2.80 g, 13.37 mmol) followed by triethylamine (1.69 mL, 12.16 mmol). The reaction mixture was stirred for 15 hours at reflux temperature after which time the reaction was concentrated under reduced pressure and purified by column chromatography on silica gel using a CHCl 3 /MeOH (99/1) mixture as eluent to give the title product as a white solid ([2H] To a solution of the 1,2-hydrazodicarboxamide [2H]-2 (2.87 g, 5.71 mmol) in dichloromethane (50 mL) were added pyridine (0.6 mL, 6.85 mmol) and N-bromosuccinimide (1.12 g, 6.28 mmol). The resulting orange solution was stirred at room temperature for 1 h. Then the reaction mixture was diluted with dichloromethane (100 mL) and sequentially washed with water (100 mL), 5% aqueous solution of Na 2 S 2 O 3 (75 mL) and saturated solution of NaHCO 3 (2 × 75 mL). The organic phase was dried with anhydrous MgSO 4 , and concentrated under reduced pressure to afford a crude material which was purified by column chromatography on silica gel eluting with a CHCl 3 /MeOH (98/2) mixture as eluent to give the title product as an orange solid (2, 2.

Synthesis of Rotaxane 4
To a solution of the [2]rotaxane 3 (15 mg, 0.014 mmol) in chloroform (0.5 mL) was added m-CPBA (7.5 mg, 3 equiv.). The resulting solution was stirred at 25 • C for 2 h. Then the reaction mixture was diluted with chloroform (5 mL) and sequentially washed with a saturated solution of NaHCO 3 (2 × 10 mL) and brine (10 mL). The organic phase was dried with MgSO 4 , and concentrated under reduced pressure to afford the [2]

Synthesis of Rotaxane 5
To a solution of the [2]rotaxane 3 (10 mg, 0.01 mmol) in chloroform (0.5 mL) was added trifluoroacetic acid (2.2 µL, 3 equiv.). The resulting solution was stirred at room temperature for 1 h. Then the solvent and excess of TFA were removed under vacuum, affording the [2]

Synthesis of Rotaxane 6
To a solution of the [2]rotaxane 3 (15 mg, 0.014 mmol) in acetonitrile (0.5 mL) was added allyl iodide (7.3 mg, 3 equiv.). The resulting solution was stirred at 70 • C for 48 h. Then the solvent was removed under vacuum. The resulting residue was washed with Et 2 O and CHCl 3 to afford the [2]

Conclusions
In summary, we have described the assembly of two hydrogen-bonded [2]rotaxanes having a nitrogenated binding site and two pyridine rings in the macrocycle. A close inspection of their 1 H NMR spectra reveals a deep dissimilarity in the dynamic behavior of the cyclic component as consequence of the distinguishable affinity of the binding sites for the macrocycle and the non-covalent interactions between both interlocked components. The chemical interconversion between the azo and hydrazo forms of the [2]-rotaxane facilitated the development of a chemically-switchable interlocked system enable to exchange two different dynamic states as a function of the oxidation level of the binding site of one of the entwined components. Moreover, we functionalized the pyridine-based macrocycle by oxidation, protonation and alkylation reactions, finding a new chemical means of exchange the dynamics of such interlocked systems.