1,4-Naphthoquinone Triggers Nematode Lethality by Inducing Oxidative Stress and Activating Insulin/IGF Signaling Pathway in Caenorhabditis elegans

Plant-parasitic nematodes are destructive pathogens causing enormous economic losses worldwide. With the withdrawal of fumigants, organophosphates and carbamates, pathogenic nematode control is more difficult. Phytochemicals are the plant secondary metabolites and are friendly for men and the environment. For developing new nematocidal candidates, we screened 790 phytochemicals using the model organism Caenorhabditis elegans and found 10 active compounds, 3 of which were further evaluated for their inhibitory activities against egg hatching of C. elegans and J2 Meloidogyne incognita. Among them, 1,4-naphthoquinone (1,4-NQ) was the only compound that could kill more than 50% of targets at 50 μg/mL, prompting us to investigate how 1,4-NQ triggers nematode lethality. In C. elegans, we observed that 1,4-NQ could influence reactive oxygen production, superoxide dismutase activity, and the heat-shock transcription factor (HSF)-1 pathway, which indicated that 1,4-NQ stimulated significant oxidative stress. Furthermore, using quantitative RT-PCR and transgenetic nematodes, we revealed that 1,4-NQ lethality was related to the Insulin/IGF signaling (IIS) pathway, and the effect of 1,4-NQ on IIS pathway related genes indicated that 1,4-NQ could activate this pathway and suppress the expression of DAF-16 target genes. The triggering of oxidative stress and activation of the IIS pathway indicated that 1,4-NQ operates through the generation of oxygen radicals, which can be lethal to C. elegans, thus making it an interesting lead compound for the development of future nematocides.


Introduction
Plant-parasitic nematode infections cause huge economic losses in global agriculture, which are estimated nearly at $157 billion annually [1,2]. Although, synthetic nematocides, such as fumigants, organophosphates, and carbamates, are effective for nematode control, their use is becoming increasingly restricted due to their side effects to the environment and human health, thus increasing the demand for more sustainable nematocides [3]. To develop reliable and effective nematocides, many studies focused on plant-parasitic nematode control, possessing lower influences on non-target

1,4-NQ Stimulates ROS Production and Suppresses Elimination of ROS in C. elegans
Xenobiotic exposure may cause excess ROS production in cells and tissues, and induce intracellular redox homeostasis, such as irreversible oxidative modifications of lipid, protein, or DNA [20]. Previous studies revealed that quinones are redox cyclers and have the ability to stimulate ROS production in organisms. For example, 5-hydroxy-1,4-naphthoquinone (juglone) and 2-methyl-1,4-naphthoquinone (menadione, MQ) are used as ROS generators to induce oxidative stress in animals [21,22]. Thus, we used an indicator dye carboxy-H2DCFDA to measure the ROS levels of C. elegans exposed to various concentrations of 1,4-NQ or MQ, a positive control. Compared with the control, 1,4-NQ could excessively stimulate ROS generation in worms ( Figure  2A,B). SOD is an anti-oxidant enzyme that converts superoxide to hydrogen peroxide and protects animals from ROS damage [23], so the influence of 1,4-NQ on SOD activity was investigated. The one-way ANOVA indicated that 1,4-NQ significantly altered SOD activity [F(2,6) = 5.612, p < 0.05]. As shown in Figure 2C, SOD activity was significantly decreased after treating N2 L1 nematodes with 25 μg/mL 1,4-NQ. Taken together, these results indicated that 1,4-NQ not only stimulates ROS production but suppresses the elimination of ROS.

1,4-NQ Stimulates ROS Production and Suppresses Elimination of ROS in C. elegans
Xenobiotic exposure may cause excess ROS production in cells and tissues, and induce intracellular redox homeostasis, such as irreversible oxidative modifications of lipid, protein, or DNA [20]. Previous studies revealed that quinones are redox cyclers and have the ability to stimulate ROS production in organisms. For example, 5-hydroxy-1,4-naphthoquinone (juglone) and 2-methyl-1,4-naphthoquinone (menadione, MQ) are used as ROS generators to induce oxidative stress in animals [21,22]. Thus, we used an indicator dye carboxy-H 2 DCFDA to measure the ROS levels of C. elegans exposed to various concentrations of 1,4-NQ or MQ, a positive control. Compared with the control, 1,4-NQ could excessively stimulate ROS generation in worms (Figure 2A,B). SOD is an anti-oxidant enzyme that converts superoxide to hydrogen peroxide and protects animals from ROS damage [23], so the influence of 1,4-NQ on SOD activity was investigated. The one-way ANOVA indicated that 1,4-NQ significantly altered SOD activity [F (2,6) = 5.612, p < 0.05]. As shown in Figure 2C, SOD activity was significantly decreased after treating N2 L1 nematodes with 25 µg/mL 1,4-NQ. Taken together, these results indicated that 1,4-NQ not only stimulates ROS production but suppresses the elimination of ROS.

1,4-NQ Activates HSF-1 Signaling Pathway in C. elegans
Heat shock proteins (HSPs) are chaperones that refold or recycle damaged proteins and inhibit protein aggregation [24,25]. As previously reported, redundant ROS could cause protein damage and aggregation, and activate heat-shock transcription factor (HSF)-1 pathway that regulates the expression of HSPs [20,24]. Therefore, the effect of 1,4-NQ on the expressions of HSF-1 pathway related genes was evaluated. Gene expressions were significantly altered among all experimental groups after 1,4-  respectively. (C) 1,4-NQ suppresses SOD activity. Wild type N2 L1s were treated with 0, 12.5 and 25 µg/mL 1,4-NQ for 2 h. The worm lysates were prepared and subjected to analysis of SOD using assay kits. Data presented as mean ± SEM and each experiment was repeated at least three times. Significant differences between the control group and 1,4-NQ treated group, * p < 0.05 (one-way ANOVA followed by an LSD test). Wild type N2 L1s were treated with 0, 12.5 and 25 μg/mL 1,4-NQ for 2 h. The worm lysates were prepared and subjected to analysis of SOD using assay kits. Data presented as mean ± SEM and each experiment was repeated at least three times. Significant differences between the control group and 1,4-NQ treated group, * p < 0.05 (one-way ANOVA followed by an LSD test).

1,4-NQ Activates HSF-1 Signaling Pathway in C. elegans
Heat shock proteins (HSPs) are chaperones that refold or recycle damaged proteins and inhibit protein aggregation [24,25]. As previously reported, redundant ROS could cause protein damage and aggregation, and activate heat-shock transcription factor (HSF)-1 pathway that regulates the expression of HSPs [20,24]. Therefore, the effect of 1,4-NQ on the expressions of HSF-1 pathway related genes was evaluated. Gene expressions were significantly altered among all experimental groups after 1,4-  Wild type N2 L1s were incubated with various concentrations of 1,4-NQ for 12 h, the expression of the HSF-1 pathway related genes, hsf-1, hsp-16.1, hsp-16.49, sip-1, and daf-21 was measured by quantitative RT-PCR and normalized to act-1 expression. Data presented as mean ± SEM and each experiment was repeated at least three times. Significant differences between the control group and 1,4-NQ treated group, *** p < 0.001, ** p < 0.01, * p < 0.05 (one-way ANOVA followed by an LSD test).
expression. Data presented as mean ± SEM and each experiment was repeated at least three times. Significant differences between the control group and 1,4-NQ treated group, *** p < 0.001, ** p < 0.01, * p < 0.05 (one-way ANOVA followed by an LSD test).

Effect of 1,4-NQ on IIS Pathway Related Gene Expressions in C. elegans
The IIS pathway plays a key role in innate immune system in C. elegans. Therefore we investigated the effect of 1,4-NQ on the IIS pathway. age-1, involved in the IIS pathway, could suppress the nuclear localization of DAF-16, which belongs to the forkhead transcription factors of FoxO family and regulates multiple stress-related gene expression [26,27]. We evaluated the effect of 1,4-NQ on the mRNA expression levels of IIS related genes: age-1 and DAF-16 target genes (sod-3, mtl-1, ctl-2, and daf-12) [28,29]. One-way ANOVA analysis revealed that gene expressions were significantly altered among all experimental groups after 1,4-NQ treatment [F (3,8) -12]. The expression levels were all significantly increased in N2 L1 larva at 12.5 and 25 μg/mL 1,4-NQ, but were not significantly influenced at 6.25 μg/mL except that age-1 was upregulated and mtl-1 was downregulated ( Figure 4). So it can be implied that 1,4-NQ influences the expression of the IIS pathway related genes in C. elegans. were treated with various concentration of 1,4-NQ for 12 h at 20 °C, the expression of the IIS pathway related genes (age-1, sod-3, mtl-1, ctl-2, and daf-12) was measured by quantitative RT-PCR and normalized to act-1 expression. Data presented as mean ± SEM and each experiment was repeated at least three times. Significant differences between the control group and 1,4-NQ treated group, *** p < 0.001, ** p < 0.01, * p < 0.05 (one-way ANOVA followed by an LSD or a Dunnett's T3 test).

Discussion
Plants have been widely used in plant-parasitic control, but only a handful of plant-derived nematocides are used in the world. In order to develop new plant nematocidal candidates, we took advantage of the natural compound library in our lab by screening 790 phytochemicals using C. elegans or. J2 M. incognita, which led to 1,4-NQ, isolated from the roots and rhizomes of Rubia wallichiana, as the candidate for nematocidal mechanism research.
Most quinones are redox cyclers and could stimulate oxidative stress in organisms. In our study, 1,4-NQ could excessively stimulate ROS production in worms and suppress SOD activity ( Figure 2). Therefore, the outburst of oxidative stress induced by 1,4-NQ in worms may be a comprehensive consequence of ROS production and the disruption of the ROS defense system. Previous studies revealed that high level of oxidative stress could trigger cell death by inducing oxidative damage to protein, lipids, and DNA [20]. And we speculated that excessive ROS and enginery dysfunction in ROS elimination might be the main reason for the death of nematodes induced by 1,4-NQ. HSPs can bind to damaged or unfolded proteins and mediate aggregation proteins to be refolded or recycled [24,25]. And they are regulated by the HSF-1 signaling pathway which could be activated by protein damage [24]. Thus, we tested the expression levels of several HSP genes (hsf-1, hsp-16.1, hsp-16.49, sip-1, and daf-21) and all of the tested genes were upregulated after 1,4-NQ treatment (Figure 3). The requirement of HSF-1 and HSPs suggests protein damage in nematodes. Therefore, we postulate that protein damage occurs during the exposure to 1,4-NQ, which is a significant factor contributing to the death of C. elegans.

Discussion
Plants have been widely used in plant-parasitic control, but only a handful of plant-derived nematocides are used in the world. In order to develop new plant nematocidal candidates, we took advantage of the natural compound library in our lab by screening 790 phytochemicals using C. elegans or. J2 M. incognita, which led to 1,4-NQ, isolated from the roots and rhizomes of Rubia wallichiana, as the candidate for nematocidal mechanism research.
Most quinones are redox cyclers and could stimulate oxidative stress in organisms. In our study, 1,4-NQ could excessively stimulate ROS production in worms and suppress SOD activity ( Figure 2). Therefore, the outburst of oxidative stress induced by 1,4-NQ in worms may be a comprehensive consequence of ROS production and the disruption of the ROS defense system. Previous studies revealed that high level of oxidative stress could trigger cell death by inducing oxidative damage to protein, lipids, and DNA [20]. And we speculated that excessive ROS and enginery dysfunction in ROS elimination might be the main reason for the death of nematodes induced by 1,4-NQ. HSPs can bind to damaged or unfolded proteins and mediate aggregation proteins to be refolded or recycled [24,25]. And they are regulated by the HSF-1 signaling pathway which could be activated by protein damage [24]. Thus, we tested the expression levels of several HSP genes (hsf-1, hsp-16.1, hsp-16.49, sip-1, and daf-21) and all of the tested genes were upregulated after 1,4-NQ treatment (Figure 3). The requirement of HSF-1 and HSPs suggests protein damage in nematodes. Therefore, we postulate that protein damage occurs during the exposure to 1,4-NQ, which is a significant factor contributing to the death of C. elegans.
The IIS pathway, a key component of an innate immune system, is involved in stress responses, so we investigated whether 1,4-NQ influences the expression of IIS pathway related genes. We found that the mRNA levels of DAF-16 targeted genes sod-3, mtl-1, ctl-2, and daf-12 were significantly increased in N2 treated with 1,4-NQ at 12.5 and 25 µg/mL, which suggested the nuclear localization of DAF-16. However, we also discovered that age-1 was also upregulated, furthermore, after treatment with 6.25 µg/mL 1,4-NQ, age-1 gene was still upregulated and mtl-1 gene was down regulated, with other genes not significantly influenced (Figure 4). Mutants in age-1 are more resistant to ROS generator paraquat, and overexpression of age-1 might suppress nuclear localization of DAF-16 and the expression of DAF-16 target genes [26,32]. So these data suggested that the IIS pathway might be activated and the expression of DAF-16 target genes was suppressed.
To investigate whether IIS was activated by 1,4-NQ treatment, experiments were carried out to test if DAF-16 is required for 1,4-NQ-induced response. We found that compared with N2 L1s, daf-2 mutants are more resistance to 1,4-NQ, but daf-16 mutants are more sensitive ( Figure 5). We also observed a significant DAF-16 localization in nuclear after treatment of 100 µg/mL 1,4-NQ, which implied that 1,4-NQ could induce DAF-16 nuclear localization ( Figure 6). All of these indicated that DAF-16 is required for nematode resistance against 1,4-NQ.
Previous studies showed that DAF-16/FoxO is not only regulated by the IIS pathway, but it also can be activated by other activators, such as oxidative stress and HSF-1 [28,33]. In other words, damage not only occurs through the direct detrimental effects of xenobiotics, but also through the side effect elicited by the defense responses toward xenobiotic. Thus, we could speculate that DAF-16 localization was modulated by oxidative stress and the HSF-1 signaling pathway that was induced by high concentration of 1,4-NQ treatment (Figure 2), which resulted in resistance of daf-2 mutants against 1,4-NQ and nuclear localization of DAF-16. Thus, the suppressive activity of age-1 on DAF-16 nuclear localization was overlapped by other DAF-16 activators, which resulted in the upregulation of age-1 and DAF-16 target genes at 12.5 and 25 µg/mL. However, at a low concentration, modulation of other activators on DAF-16 nuclear localization is not obvious and the effect of 1,4-NQ on the IIS pathway's activating is emerging, which results in the upregulation of age-1 and down regulation of the DAF-16 target gene mtl-1. Taken together, 1,4-NQ probably activated the IIS pathway and resulted in suppression of DAF-16 nuclear localization, thereby contributing to nematode death.
In conclusion, we screened 790 phytochemicals using C. elegans or J2 M. incognita and revealed the nematocidal mechanism of candidate 1,4-NQ (Figure 7). In this work, we found that 1,4-NQ exerts nematocidal activity by inducing oxidative stress and activating the IIS pathway to suppress resistant gene expression, which may be developed into a more environmentally friendly nematocides. The IIS pathway, a key component of an innate immune system, is involved in stress responses, so we investigated whether 1,4-NQ influences the expression of IIS pathway related genes. We found that the mRNA levels of DAF-16 targeted genes sod-3, mtl-1, ctl-2, and daf-12 were significantly increased in N2 treated with 1,4-NQ at 12.5 and 25 μg/mL, which suggested the nuclear localization of DAF-16. However, we also discovered that age-1 was also upregulated, furthermore, after treatment with 6.25 μg/mL 1,4-NQ, age-1 gene was still upregulated and mtl-1 gene was down regulated, with other genes not significantly influenced (Figure 4). Mutants in age-1 are more resistant to ROS generator paraquat, and overexpression of age-1 might suppress nuclear localization of DAF-16 and the expression of DAF-16 target genes [26,32]. So these data suggested that the IIS pathway might be activated and the expression of DAF-16 target genes was suppressed.
To investigate whether IIS was activated by 1,4-NQ treatment, experiments were carried out to test if DAF-16 is required for 1,4-NQ-induced response. We found that compared with N2 L1s, daf-2 mutants are more resistance to 1,4-NQ, but daf-16 mutants are more sensitive ( Figure 5). We also observed a significant DAF-16 localization in nuclear after treatment of 100 μg/mL 1,4-NQ, which implied that 1,4-NQ could induce DAF-16 nuclear localization ( Figure 6). All of these indicated that DAF-16 is required for nematode resistance against 1,4-NQ.
Previous studies showed that DAF-16/FoxO is not only regulated by the IIS pathway, but it also can be activated by other activators, such as oxidative stress and HSF-1 [28,33]. In other words, damage not only occurs through the direct detrimental effects of xenobiotics, but also through the side effect elicited by the defense responses toward xenobiotic. Thus, we could speculate that DAF-16 localization was modulated by oxidative stress and the HSF-1 signaling pathway that was induced by high concentration of 1,4-NQ treatment (Figure 2), which resulted in resistance of daf-2 mutants against 1,4-NQ and nuclear localization of DAF-16. Thus, the suppressive activity of age-1 on DAF-16 nuclear localization was overlapped by other DAF-16 activators, which resulted in the upregulation of age-1 and DAF-16 target genes at 12.5 and 25 μg/mL. However, at a low concentration, modulation of other activators on DAF-16 nuclear localization is not obvious and the effect of 1,4-NQ on the IIS pathway's activating is emerging, which results in the upregulation of age-1 and down regulation of the DAF-16 target gene mtl-1. Taken together, 1,4-NQ probably activated the IIS pathway and resulted in suppression of DAF-16 nuclear localization, thereby contributing to nematode death.
In conclusion, we screened 790 phytochemicals using C. elegans or J2 M. incognita and revealed the nematocidal mechanism of candidate 1,4-NQ (Figure 7). In this work, we found that 1,4-NQ exerts nematocidal activity by inducing oxidative stress and activating the IIS pathway to suppress resistant gene expression, which may be developed into a more environmentally friendly nematocides.

Chemical Compounds
All of these chemicals were obtained from the library of phytochemicals in our lab. 1,4-Naphthoquinone was isolated from the roots and rhizomes of Rubia wallichiana. Menadione was purchased from Aladdin Industrial Cooperation, Shanghai, China.

Nematode Strains and Culture Methods
Strains N2 (Bristol, wild type), CB1370 daf-2(e1370)III., CF1038 daf-16(mu86)I. and TJ356 daf-16(zls356)IV. were purchased from Caenorhabditis Genetics Center (CGC). All strains were maintained at 20 • C and grew on nematode growth medium (NGM) plates seeded with Escherichia coli OP50 [34]. Embryos were collected after gravid nematodes were treated with hypochlorite bleaching. After hatching for 12 h, L1 nematodes were moved to NGM plates with Escherichia coli OP50, L2-L3 larva were then collected after being cultured for about 24 h and L4 worms were collected after being cultured for about 48 h [35].
Infected soil was obtained from Yuxi, Yunnan Province, China and used to establish a population of M. incognita on tomato. Egg sacks were picked from heavily infected roots using a needle. These egg sacks were sterilized by 1% NaClO, and then washed three times in sterilized water. The sterilized egg sacks were placed on a 9 cm diameter sterile Petri dish and incubated at 28 • C in sterilized water. After hatching for 48 h, second juveniles J2 were removed and collected for liquid-based chemical screening.

Chemical Screening
Screening and rescreening: wild type N2 L4 worms were collected in M9 buffer and arrayed at 70 nematodes per well in 96-well plates, 100 µL per well. Chemicals were added to each well at a final concentration of 50 µg/mL (0.5% v/v DMSO), with DMSO as controls. Evaluation screening: wild type N2 L4 or J2 M. incognita were collected in M9 buffer or water and arrayed at 300 nematodes per well in 24-well plates, 400 µL per well. Chemicals were added to each well at a final concentration of 0, 3.125, 6.25, 12.5, 25 and 50 µg/mL (0.5% v/v DMSO), with DMSO as controls. Egg hatching assay: wild type N2 embryos were collected immediately after bleaching and dispensed into 24-well plates with 300 eggs, 400 µL per well, with a final concentration of 0, 3.125, 6.25, 12.5, 25 and 50 µg/mL (0.5% v/v DMSO), with DMSO as controls. All the results were conducted after 48 h exposure of phytochemicals.

ROS Measurement
Synchronized wild type N2 L1 nematodes were treated 0, 25, 50 µg/mL 1,4-NQ or 50 µg/mL MQ (positive control) for 2, 4, 8, and 12 h, respectively, and then washed three times in M9 buffer. The washed test worms were transferred to 1 mL of M9 buffer containing 1µM carboxy-H 2 DCFDA. After incubation for 30 min, nematodes were washed three times in M9 buffer, and then mounted on a glass slide for examination with a laser scanning confocal microscope (Leica, Leica DM5500B, Bensheim, Germany) at 488 nm of excitation wavelength and 510 nm of emission filter. The fluorescent intensities were semi-quantified as relative fluorescent units (RFU). More than 50 worms were examined per replicate.

Measurement of SOD Activity
About 50,000 synchronized wild type N2 L1 nematodes were treated with 0, 12.5, and 25 µg/mL 1,4-NQ, respectively and cultured at 20 • C for 2 h. Then, the worms were washed three times in M9 buffer. The worms were collected and lysed for analysis of SOD. SOD activity was measured at 450 nm following the manufacturer's protocols of commercially available kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).

Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analyses
About 100,000 synchronized wild type N2 L1 nematodes were treated with 0, 6.25, 12.5, and 25 µg/mL 1,4-NQ and cultured at 20 • C. After treatment for 12 h, worms were washed three times in M9 buffer and collected for RNA isolation. Total RNA was isolated using Trizol reagent (Invitrogen Corp., Carlsbad, CA, USA) and converted to cDNA using PrimeScript™ RT reagent Kit (TaKaRa, Tokyo, Japan). The RT-PCR was performed using iTaq™ Universal SYBR ® Green Supermix (Bio-rad) and Bio-Rad CFX Manager 3.1. Primer sequences are listed in Table S1.

Statistical Analysis
All experiment data was repeated at least three times and expressed as mean ± SEM. Data was analyzed using SPSS 16.0 (SPSS Inc., Chicago, IL, USA). Statistical significance was estimated with a one-way ANOVA followed by an LSD (equal variances assumed) or a Dunnett's T3 (equal variances not assumed) post-hoc test to determine significant differences between groups. p < 0.05 was considered as significant value.