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Molecules 2017, 22(12), 2234; https://doi.org/10.3390/molecules22122234

rLj-RGD3 Suppresses the Growth of HeyA8 Cells in Nude Mice

1,†
,
2,†
,
1
,
1
,
1,* and 1,*
1
School of Life Sciences, Liaoning Normal University, Dalian 116081, China
2
Department of Pharmacology, Dalian Medical University, Dalian 116044, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 1 November 2017 / Revised: 1 December 2017 / Accepted: 12 December 2017 / Published: 15 December 2017
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Abstract

In the previous study, rLj-RGD3, a recombinant toxin protein which contains three RGD motifs, was reported to not only inhibit the proliferation of an ovarian cancer cell line, HeyA8 cells, by inducing apoptosis, but also block their adhesion, migration and invasion processes. However, whether rLj-RGD3 could also suppress the tumor growth in HeyA8 xenografted mice has not been reported yet. In the present study, rLj-RGD3 was intraperitoneally injected in the nude mice bearing HeyA8 tumors. Compared with the control group (normal saline), rLj-RGD3 inhibited the tumor growth significantly in the HeyA8 xenografted mice in a dose-dependent manner without affecting their body weights. Based on the H&E, Hoechst 33258 and TUNEL staining assays, as well as western blot analysis, rLj-RGD3 reduced the weight and volume of the solid tumors, probably by disturbing the tissue structure, inducing apoptosis and suppressing the FAK/PI3K/AKT pathway. Most importantly, rLj-RGD3 was found to prolong the survival days of the ovarian tumor xenografted mice, which suggested rLj-RGD3 might act as an effective and safe drug to treat ovarian cancer patients. View Full-Text
Keywords: rLj-RGD3; HeyA8 cells; nude mice; PI3K; AKT rLj-RGD3; HeyA8 cells; nude mice; PI3K; AKT
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Zheng, Y.; Lv, L.; Yi, L.; Wu, R.; Xiao, R.; Wang, J. rLj-RGD3 Suppresses the Growth of HeyA8 Cells in Nude Mice. Molecules 2017, 22, 2234.

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