New Tripentone Analogs with Antiproliferative Activity

Tripentones represent an interesting class of compounds due to their significant cytotoxicity against different human tumor cells in the submicro-nanomolar range. New tripentone analogs, in which a pyridine moiety replaces the thiophene ring originating the fused azaindole system endowed with anticancer activity viz 8H-thieno[2,3-b]pyrrolizinones, were efficiently synthesized in four steps with fair overall yields (34–57%). All tripentone derivatives were tested in the range of 0.1–100 μM for cytotoxicity against two human tumor cell lines, HCT-116 (human colorectal carcinoma) and MCF-7 (human breast cancer). The most active derivative, with GI50 values of 4.25 µM and 20.73 µM for HCT-116 and MCF-7 cells, respectively, did not affect the viability of Caco-2 differentiated in normal intestinal-like cells, suggesting tumor cells as the main target of its cytotoxic action. The same compound was further investigated in order to study its mode of action. Results showed that it did not exert necrotic effects, while induced a clear shift of viable cells towards early apoptosis. Flow cytometric analysis demonstrated that this compound caused cell cycle alteration, inhibiting its progression in S and G2/M phases.


Introduction
Cancer is one of the most common causes of morbidity representing an important social problem supposed to maintain its primacy after heart and circulatory disorders, with many new cases in the world per year [1,2]. In most cases the prognosis is relatively poor; in fact, although in recent years several improvements in treatment and prevention have been made, the number of new cases is still increasing. For these reasons and considering the fact that chemotherapy represents the most effective strategy to fight cancer, in the recent years many efforts have been made to find new and effective anticancer molecules [3][4][5][6][7][8].
Pyrrole-fused indoles and related aza-derivatives have emerged as an interesting class of anti-cancer agents endowed with potent activity against different human tumor cell lines and with different mechanisms of action [9][10][11][12][13][14][15][16][17][18][19][20]. In particular, 8H-thieno [2,3-b]pyrrolizinones, also known as "tripentones" (Chart 1), have been shown to demonstrate significant cytotoxicity against tumor cells in the submicro-nanomolar range [21,22]. Different tripentone analogs have been synthesized; in particular, 3-aryl-8H-thieno [2,3-b]pyrrolizin-9-ones 1, tested on 60 tumor cell lines (National Cancer Institute, NCI), showed high cytotoxicity [21] and selectivity against the leukemia subpanel [22]. most active compound, MR16924, showing IC50 values in the submicromolar range, was elected as the lead for the synthesis of different analogs in which the thiophene ring was replaced by bioisosteric analogs such as furan (2), pyrrole (3), other thiophene isomers (4,5) [22,23], pyrazole (6) [24], or in which benzene (7) [25] or the indole ring (8) were added [26] (Chart 1). Biological results confirmed the importance of the thienopyrrolizinone core, although many derivatives exhibited interesting cytotoxic properties. The most active compound, MR22388, a thieno [2,3-b]pyrrolizin-9-one derivative, showed significant antitumor activity in different human tumor cell lines with IC50 values in the submicromolar or nanomolar range. Biological studies performed on this compound demonstrated that it acts as a pro-apoptotic agent, causing cell cycle arrest at the G2/M phase and increasing caspase 3 activity. Further studies, performed in order to clarify its mechanism of action, showed that it causes the inhibition of tubulin polymerization with an IC50 value of 2.9 μM, as well as the activation of several kinases including FLT3-ITD [27]. Compound MR22388 was also investigated in vivo, but the results were less exciting than those obtained in the in vitro studies, probably due to the poor bioavailability of the tested drug [22]. Continuing on our ongoing studies on nitrogen heterocyclic systems endowed with antitumor activity [28][29][30][31][32][33][34][35], and considering that hydrazide chains could improve the potency and pharmacokinetic properties of compounds bearing them [36], herein we report the synthesis of new 9H-pyrido[2,3-b]pyrrolizin-9-one tripentone analogs 9, in which a pyridine replaces the thiophene ring of 8H-thieno [2,3-b]pyrrolizinones and in which different carbohydrazine chains were inserted with the aim of endowing them with the proper pharmacokinetic properties responsible for biological activity.

Chemisty
Tripentone compounds were synthesized as described in Scheme 1. The synthetic pathway starts from compound 10, prepared from the corresponding 3-aminopicolinic acid as described in the literature [37]. The latter was reacted under Clauson-Kaas conditions in the presence of 2,5dimethoxytetrahydrofuran, 4-chloropyridine hydrochloride in anhydrous 1,4-dioxane at reflux to give, in excellent yields (92%), the pyrrolidine derivative 11, which was then converted to its corresponding amide 12. The first attempt was made in refluxing pyrrolidine in a ratio up to 1:42 for 36 h, but unfortunately the desired amide was obtained in low yield (38%). We decided to try a different method via carboxylic acid, using the activating agent of the carboxylic function N-(3-Chart 1. Tripentone analogs.
Continuing on our ongoing studies on nitrogen heterocyclic systems endowed with antitumor activity [28][29][30][31][32][33][34][35], and considering that hydrazide chains could improve the potency and pharmacokinetic properties of compounds bearing them [36], herein we report the synthesis of new 9H-pyrido[2,3-b]pyrrolizin-9-one tripentone analogs 9, in which a pyridine replaces the thiophene ring of 8H-thieno [2,3-b]pyrrolizinones and in which different carbohydrazine chains were inserted with the aim of endowing them with the proper pharmacokinetic properties responsible for biological activity.

Chemisty
Tripentone compounds were synthesized as described in Scheme 1. The synthetic pathway starts from compound 10, prepared from the corresponding 3-aminopicolinic acid as described in the literature [37]. The latter was reacted under Clauson-Kaas conditions in the presence of 2,5-dimethoxytetrahydrofuran, 4-chloropyridine hydrochloride in anhydrous 1,4-dioxane at reflux to give, in excellent yields (92%), the pyrrolidine derivative 11, which was then converted to its corresponding amide 12. The first attempt was made in refluxing pyrrolidine in a ratio up to 1:42 for 36 h, but unfortunately the desired amide was obtained in low yield (38%). We decided to try a different method via carboxylic acid, using the activating agent of the carboxylic function N-(3-dimethylaminopropyl)-N -ethylcarbodiimide hydrochloride (EDC) and hydroxy-benzotriazole (HOBt). The corresponding carboxylic acid was obtained by hydrolysis of the ester group under basic conditions, using lithium hydroxide (LiOH) in ethanol at reflux for 4 h. Subsequent amidation with pyrrolidine, in the presence of EDC, HOBt, and N,N-diisopropylethylamine (DIPEA), in tetrahydrofuran at room temperature for 12 h afforded the desired amide 12, isolated in high yield (88%) and in a shorter time. Cyclization of the latter was performed by acylation under Vilsmeier-Haack conditions followed by alkaline treatment. Reaction of the amide 12 with phosphorous oxychloride (POCl3) afforded an intermediate iminium salt that was subsequently hydrolyzed (NaOH 10%) to give tripentone 9a isolated in good yield (70%, Table 1). The latter was used for the successive reactions of substitution with suitable heteroaryl carbohydrazide side chains, performed in toluene or ethanol under reflux, to give derivatives 9b-h (60-93%, Table 1). Pyridin-4-yl 69 9c Pyridin-3-yl 93 9d Furan-2-yl 72 9e Thiophen-2-yl 70 9f 4-NH2-phenyl 60 9g 4-OH-phenyl 90 9h Phenyl 76

Cytotoxic Activity
All synthesized tripentone derivatives 9a-h were tested in the concentration range of 0.1-100 μM for the evaluation of cytotoxicity against two human tumor cell lines, HCT-116 (human colorectal carcinoma) and MCF-7 (human breast cancer), by MTT assay. Compounds 9a-g did not substantially affect the tumor cells viability, whereas 9h effectively inhibited the growth of both cell lines (Figure Cyclization of the latter was performed by acylation under Vilsmeier-Haack conditions followed by alkaline treatment. Reaction of the amide 12 with phosphorous oxychloride (POCl 3 ) afforded an intermediate iminium salt that was subsequently hydrolyzed (NaOH 10%) to give tripentone 9a isolated in good yield (70%, Table 1). The latter was used for the successive reactions of substitution with suitable heteroaryl carbohydrazide side chains, performed in toluene or ethanol under reflux, to give derivatives 9b-h (60-93%, Table 1).

Cytotoxic Activity
All synthesized tripentone derivatives 9a-h were tested in the concentration range of 0.1-100 µM for the evaluation of cytotoxicity against two human tumor cell lines, HCT-116 (human colorectal carcinoma) and MCF-7 (human breast cancer), by MTT assay. Compounds 9a-g did not substantially affect the tumor cells viability, whereas 9h effectively inhibited the growth of both cell lines ( Figure 1A,B). Calculated GI 50 values, i.e., the molar concentration of the compound that inhibits 50% cell growth, were 4.25 ± 0.31 µM and 20.73 ± 1.22 µM for HCT-116 and MCF-7 cells, respectively.
Interestingly, derivative 9h had no effect on the vitality of Caco-2 differentiated in normal intestinal-like cells, suggesting tumor cells as the main target of its cytotoxic action ( Figure 1C). 1A,B). Calculated GI50 values, i.e., the molar concentration of the compound that inhibits 50% cell growth, were 4.25 ± 0.31 μM and 20.73 ± 1.22 μM for HCT-116 and MCF-7 cells, respectively. Interestingly, derivative 9h had no effect on the vitality of Caco-2 differentiated in normal intestinal-like cells, suggesting tumor cells as the main target of its cytotoxic action ( Figure 1C)

Cell Death
To investigate the effects of the inhibition on cell growth (necrosis or apoptosis) of the active synthesized tripentone 9h, flow cytometry analysis of annexin V-FITC and propidium iodide (PI)stained HCT-116 and MCF-7 cells was carried out to evaluate the externalization of plasma membrane phosphatidylserine, a reliable marker of cell apoptosis. The concentration chosen for this study was selected taking into account the value of GI50 measured in both cell lines. As shown in Figure 2, the tripentone derivative 9h did not exert necrotic effects, but induced a clear shift of viable cells towards early apoptosis.

Cell Death
To investigate the effects of the inhibition on cell growth (necrosis or apoptosis) of the active synthesized tripentone 9h, flow cytometry analysis of annexin V-FITC and propidium iodide (PI)-stained HCT-116 and MCF-7 cells was carried out to evaluate the externalization of plasma membrane phosphatidylserine, a reliable marker of cell apoptosis. The concentration chosen for this study was selected taking into account the value of GI 50 measured in both cell lines. As shown in Figure 2, the tripentone derivative 9h did not exert necrotic effects, but induced a clear shift of viable cells towards early apoptosis.

Cell Cycle Analysis
Apoptosis of the tumor cells induced by the synthesized 9h derivative may be due to abnormal regulation of the cell cycle. To test this possibility, we performed a flow cytometric analysis of PIstained HCT-116 and MCF-7 cells after 24 h of treatment with the compound at the relevant GI50 value. As shown in Figure 3, 9h caused cell cycle alteration in both tumor cell lines, inducing a block of the viable cells in phase G0/G1 compared to the control and inhibiting the progression of the cell cycle in S and G2/M phases. The appearance of a sub-G1-cell population, which is representative of cells with fragmented DNA, was also evident and consistent with the apoptotic activity of the compound ( Figure  3).
These data suggest that tripentone derivative 9h induces a failure of DNA synthesis, hindering G1/S transition of the cell cycle. Then, different from other tripentones [22], the new synthesized 9h compound does not appear to be endowed with antitubulin activity, which is responsible for the mitotic failure and arrest in the G2/M phase of the cell cycle.

Cell Cycle Analysis
Apoptosis of the tumor cells induced by the synthesized 9h derivative may be due to abnormal regulation of the cell cycle. To test this possibility, we performed a flow cytometric analysis of PI-stained HCT-116 and MCF-7 cells after 24 h of treatment with the compound at the relevant GI 50 value. As shown in Figure 3, 9h caused cell cycle alteration in both tumor cell lines, inducing a block of the viable cells in phase G0/G1 compared to the control and inhibiting the progression of the cell cycle in S and G2/M phases. The appearance of a sub-G1-cell population, which is representative of cells with fragmented DNA, was also evident and consistent with the apoptotic activity of the compound (Figure 3).
These data suggest that tripentone derivative 9h induces a failure of DNA synthesis, hindering G1/S transition of the cell cycle. Then, different from other tripentones [22], the new synthesized 9h compound does not appear to be endowed with antitubulin activity, which is responsible for the mitotic failure and arrest in the G2/M phase of the cell cycle.

Chemistry
All melting points were taken on a Büchi-Tottoly capillary (Büchi, Cornaredo, Italy) apparatus and are uncorrected. IR spectra were determined in bromoform with a Shimadzu FT/IR 8400S spectrophotometer (Shimadzu Corporation, Milan, Italy). 1 H-and 13 C-NMR spectra were measured at 200 and 50.0 MHz, respectively, in DMSO-d6 or CDCl3 solution, using a Bruker Avance II series 200 MHz spectrometer (Bruker, Milan, Italy). Multiplicity of the 13 C signals were determined through DEPT spectra. Column chromatography (Sigma Aldrich, Milan, Italy) was performed with Merk silica gel 230-400 mesh ASTM or with Büchi Sepacor chromatography module (prepacked cartridge system). Elemental analyses (C, H, N) were within ±0.4% of theoretical values and were performed with a VARIO EL III elemental analyzer (Elementar, Langenselbold, Germany). Purity of all the tested compounds was greater than 95%, determined by HPLC (Agilent 1100 Series) (Agilent Technologies, Milan, Italy).

Synthesis of Aminopyridine-Carboxylates (10)
Experimental procedure and spectroscopic data were in accordance with those reported [37].

Chemistry
All melting points were taken on a Büchi-Tottoly capillary (Büchi, Cornaredo, Italy) apparatus and are uncorrected. IR spectra were determined in bromoform with a Shimadzu FT/IR 8400S spectrophotometer (Shimadzu Corporation, Milan, Italy). 1 H-and 13 C-NMR spectra were measured at 200 and 50.0 MHz, respectively, in DMSO-d 6 or CDCl 3 solution, using a Bruker Avance II series 200 MHz spectrometer (Bruker, Milan, Italy). Multiplicity of the 13 C signals were determined through DEPT spectra. Column chromatography (Sigma Aldrich, Milan, Italy) was performed with Merk silica gel 230-400 mesh ASTM or with Büchi Sepacor chromatography module (prepacked cartridge system). Elemental analyses (C, H, N) were within ±0.4% of theoretical values and were performed with a VARIO EL III elemental analyzer (Elementar, Langenselbold, Germany). Purity of all the tested compounds was greater than 95%, determined by HPLC (Agilent 1100 Series) (Agilent Technologies, Milan, Italy).

Synthesis of Aminopyridine-Carboxylates (10)
Experimental procedure and spectroscopic data were in accordance with those reported [37].

Synthesis of 9H-Pyrido[2,3-b]pyrrolizin-9-one (9a)
A solution of pyrrolidinecarboxamide 12 (0.229 g, 0.95 mmol) in phosphorus oxychloride (2.4 mL, 25.65 mmol) was stirred at 70 • C for 6 h. After cooling, the reaction mixture was concentrated to give the iminium salt as a black solid. A 10% aqueous NaOH solution (6.65 mL) was slowly added to the residue and the reaction mixture was heated to 65 • C for 30 min. Upon cooling, the dark crude was extracted with ethyl acetate (3 × 15 mL), dried over anhydrous Na 2 SO 4 , and evaporated in vacuo. It was purified by silica gel column chromatography eluting by DCM:ethyl acetate, 95:5 to give the desired tripentone. To a solution of 9H-pyrido[2,3-b]pyrrolizin-9-one 9a (0.05 g, 0.29 mmol) in toluene (4 mL), the opportune heteroaryl carbohydrazide (0.29 mmol) was added. The resulting suspension was refluxed, using Dean-Stark apparatus, for 24-32 h and then chilled overnight. The product was collected by filtration, washed with toluene, and dried under vacuum to afford compound 9e. In the case of derivatives 9b,d,h, the reaction mixture was quenched with a small amount of water, then extracted with DCM (3 × 10 mL), dried over anhydrous Na 2 SO 4 , and evaporated in vacuo. It was purified by silica gel column chromatography eluting by DCM:MeOH, 97:3 (9b), DCM (9d), and DCM:ethyl acetate, 90:10 (9h) to give the desired compound. To a solution of 9H-pyrido [2,3-b]pyrrolizin-9-one 9a (0.065 g, 0.38 mmol) in anhydrous ethanol (5 mL), the opportune heteroaryl carbohydrazide (0.38 mmol) was added. The resulting solution was heated to reflux for 24 h and then chilled overnight. The product was collected by filtration, washed with cold ethanol, and dried under vacuum to afford the desired compound 9f,g, or purified by silica gel column chromatography using DCM:ethyl acetate, 75:25, as an eluent to give product 9c.

Biology
Tripentone derivatives, prepared as described above, were dissolved in dimethyl sulfoxide (DMSO) and then diluted in culture medium to obtain a DMSO concentration not exceeding 0.1%. HCT-116 (human colorectal carcinoma), MCF-7 (human breast cancer), and Caco-2 (human colorectal carcinoma) cell lines were purchased from American Type Culture Collection, Rockville, MD, USA and grown in Dulbecco's Modified Eagle's Medium(DMEM) supplemented with 10% fetal, 10% fetal bovine serum (FBS), penicillin (100 U/mL), streptomycin (100 µg/mL), and gentamicin (5 µg/mL). Cells were maintained in log phase by seeding twice a week at a density of 3 × 10 8 cells/L in a humidified 5% CO 2 atmosphere at 37 • C. In all experiments, HCT-116 and MCF-7 cells were left to incubate overnight to allow adhesion before treatment with the compounds or vehicle alone (control cells), while Caco-2 cells were treated 15 days after confluence, at which time the cells were differentiated in normal intestinal-like cells [38].
No differences were found between cells treated with DMSO 0.1% and untreated cells in terms of cell number and viability.

Viability Assay In Vitro
Cytotoxic activity of the tripentone derivatives was determined by the colorimetric assay based on the reduction of 3-(4,5-dimethyl-2-thiazolyl)bromide-2,5-diphenyl-2H-tetrazolium (MTT) to purple formazan by mitochondrial dehydrogenases [39]. Briefly, HCT-116, MCF-7, and Caco-2 lines cells were seeded at 2 × 10 4 cells/well in 96-well plates containing 200 µL DMEM. When appropriated, monolayer cultures were treated for 72 h with various concentrations (0.1-100 µM) of the tested compounds. Then cells were washed with fresh medium and 50 µL FBS-free medium containing 5 mg/mL MTT. Cells were incubated for 2 h at 37 • C, then the medium was discarded by centrifugation, formazan blue formed in the cells dissolved in DMSO, and absorbance was measured at 570 nm in a microplate reader (Bio-RAD, Hercules, CA, USA). Formazan of control cells was taken as 100% viability. The growth inhibition activity of compounds was defined as the GI 50 value, which represents the log of the molar concentration of the compound that inhibits 50% cell growth. Each experiment was repeated three times in triplicate.

Measurement of Phosphatidylserine (PS) Exposure
The apoptosis-induced PS externalization to the cell surface was measured using flow cytometry by double staining with Annexin V-Fluorescein isothiocyanate (Annexin V-FITC)/propidium iodide (PI). Annexin V binding to phosphatidylserine was used to identify the earliest stage of apoptosis. PI, which does not enter cells with intact membranes, was used to distinguish between early apoptotic cells (annexin V-FITC positive and PI negative), late apoptotic cells (annexin V-FITC/PI-double positive), and necrotic cells (annexin V-FITC negative and PI positive). After 24 h treatment, HCT-116 and MCF-7 cells were harvested by trypsinization and adjusted at 1.0 × 10 6 cells/ mL with combining buffer according to the manufacturer' instructions (eBioscience, San Diego, CA, USA). One hundred microliters of cell suspensions were added to a new tube, and incubated with Annexin V-FITC and PI solution at room temperature in the dark for 15 min. Then samples of at least 1.0 × 10 4 cells were subjected to fluorescence-activated cell sorting (FACS) analysis by an Epics XL™ flow cytometer (Beckman Coulter, Fullerton, CA, USA) using Expo 32 ACD software (Beckman Coulter, Fullerton, CA, USA), using the appropriate bi-dimensional gating method.

Cell Cycle Analysis
The cell cycle stage was analyzed by flow cytometry. HCT-116 and MCF-7 cells (5.0 × 10 4 cells/cm 2 ) were seeded in triplicate in 24-wells culture plates. After an overnight incubation, the cells were washed with fresh medium and incubated with compound 9h in DMEM for 24 h. Then cells were harvested by trypsinization. Aliquots of 1.0 × 10 6 cells were washed with PBS and incubated in the dark in a PBS solution containing 20 µg/mL PI and 200 µg/mL RNase, for 30 min at room temperature. Then samples of at least 1.0 × 10 4 cells were subjected to FACS analysis.

Conclusions
In conclusion, eight new tripentones analogs were efficiently synthesized using a four-step sequence with fair overall yields (34-57%). All synthesized derivatives were tested for cytotoxicity against two human tumor cell lines, HCT-116 (human colorectal carcinoma) and MCF-7 (human breast cancer), by MTT assay. On the basis of the obtained results, the most active derivative was further investigated to study its mode of action. Flow cytometric analysis showed that it did not exert necrotic effects, but induced a clear shift of viable cells towards early apoptosis, causing the inhibition of the cell cycle progression in S and G2/M phases.
Author Contributions: Barbara Parrino, Salviana Ullo, Virginia Spanò, Stella Cascioferro, Alessandra Montalbano, and Paola Barraja performed chemical research and analyzed the data. Alessandro Attanzio and Luisa Tesoriere performed biological research and analyzed the data. Girolamo Cirrincione, Patrizia Diana, Luisa Tesoriere, and Barbara Parrino participated in the design of the research and the writing of the manuscript. All authors read and approved the final manuscript.

Conflicts of Interest:
The authors declare no conflict of interest.