Next Article in Journal
Atom-Economic Synthesis of 4-Pyrones from Diynones and Water
Previous Article in Journal
Stepwise, Protecting Group Free Synthesis of [4]Rotaxanes
 
Search for Articles:
Title / Keyword
Author / Affiliation
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
Journals
Molecules
Volume 22
Issue 1
10.3390/molecules22010096
molecules-logo
Submit to this Journal Review for this Journal Edit a Special Issue
► Article Menu

Article Menu

share announcement question_answer thumb_up
...
textsms
...
Article

Tamaractam, a New Bioactive Lactam from Tamarix ramosissima, Induces Apoptosis in Rheumatoid Arthritis Fibroblast-Like Synoviocytes

by
Yao Yao
1,†,
Cheng-Shuai Jiang
2,†,
Na Sun
2,
Wei-Qi Li
3,
Yang Niu
4,5,
Huai-Qin Han
1,
Zhen-Hua Miao
1,
Xun-Xia Zhao
1,
Jing Zhao
6 and
Juan Li
2,7,*
1
School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, China
2
School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China
3
China National Center for Biotechnology Development, Beijing 100039, China
4
School of Chinese Medicine, Ningxia Medical University, Yinchuan 750004, China
5
Key Laboratory of Hui Medicine Modernization, Ministry of Education, Yinchuan 750004, China
6
Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China
7
Ningxia Engineering and Technology Research Center for Modernization of Hui Medicine, Yinchuan 750004, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editor: Derek J. McPhee
Molecules 2017, 22(1), 96; https://doi.org/10.3390/molecules22010096
Received: 8 November 2016 / Revised: 1 January 2017 / Accepted: 4 January 2017 / Published: 10 January 2017
(This article belongs to the Section Natural Products Chemistry)
View Full-Text Download PDF
Browse Figures

Abstract

Chemical investigation of Tamarix ramosissima Ledeb, a traditional herbal medicine used for rheumatoid arthritis (RA) treatment in northwest China, led to the discovery of a new phenolic aromatic rings substituted lactam, tamaractam (1), together with the previously reported compounds cis-N-feruloyl-3-O-methyldopamine (2) and trans-N-feruloyl-3-O-methyldopamine (3). The structures of the compounds were determined by high resolution electrospray ionization mass spectroscopy (HRESIMS) and 1D and 2D-NMR experiments, as well as comparison with the literature data. The effects of the three compounds on the viability of RA fibroblast-like synoviocytes (RA-FLS) were assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Pro-apoptosis effect of compound 1 in RA-FLS was further investigated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay, activated caspase-3/7 level assessment using luminescence assay, and sub-G1 fraction measurement using flow cytometry. It was found that these three compounds displayed variable proliferation inhibitory activity in RA-FLS, and compound 1 exhibited the strongest effect. Compound 1 could remarkably induce cellular apoptosis of RA-FLS, increase activated caspase-3/7 levels, and significantly increase sub-G1 fraction in the cell cycle. The results suggested that compound 1 may inhibit the proliferation of RA-FLS through apoptosis-inducing effect, and these compounds may contribute to the anti-RA effect of T. ramosissima. View Full-Text
Keywords: Tamarix ramosissima; tamaractam; rheumatoid arthritis; fibroblast-like synoviocytes; growth inhibitory activity; apoptosis Tamarix ramosissima; tamaractam; rheumatoid arthritis; fibroblast-like synoviocytes; growth inhibitory activity; apoptosis
►▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Supplementary Material

SciFeed

Share and Cite

MDPI and ACS Style

Yao, Y.; Jiang, C.-S.; Sun, N.; Li, W.-Q.; Niu, Y.; Han, H.-Q.; Miao, Z.-H.; Zhao, X.-X.; Zhao, J.; Li, J. Tamaractam, a New Bioactive Lactam from Tamarix ramosissima, Induces Apoptosis in Rheumatoid Arthritis Fibroblast-Like Synoviocytes. Molecules 2017, 22, 96. https://doi.org/10.3390/molecules22010096

AMA Style

Yao Y, Jiang C-S, Sun N, Li W-Q, Niu Y, Han H-Q, Miao Z-H, Zhao X-X, Zhao J, Li J. Tamaractam, a New Bioactive Lactam from Tamarix ramosissima, Induces Apoptosis in Rheumatoid Arthritis Fibroblast-Like Synoviocytes. Molecules. 2017; 22(1):96. https://doi.org/10.3390/molecules22010096

Chicago/Turabian Style

Yao, Yao, Cheng-Shuai Jiang, Na Sun, Wei-Qi Li, Yang Niu, Huai-Qin Han, Zhen-Hua Miao, Xun-Xia Zhao, Jing Zhao, and Juan Li. 2017. "Tamaractam, a New Bioactive Lactam from Tamarix ramosissima, Induces Apoptosis in Rheumatoid Arthritis Fibroblast-Like Synoviocytes" Molecules 22, no. 1: 96. https://doi.org/10.3390/molecules22010096

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Map by Country/Region

1
Back to TopTop