Preclinical Study of Antineoplastic Sinoporphyrin Sodium-PDT via In Vitro and In Vivo Models

Photodynamic therapy (PDT) investigations have seen stable increases and the development of new photosensitizers is a heated topic. Sinoporphyrin sodium is a new photosensitizer isolated from Photofrin. This article evaluated its anticancer effects by clonogenic assays, MTT assays and xenograft experiments in comparison to Photofrin. The clonogenicity inhibition rates of sinoporphyrin sodium-PDT towards four human cancer cell lines ranged from 85.5% to 94.2% at 0.5 μg/mL under 630 nm irradiation of 30 mW/cm2 for 180 s. For MTT assays, the IC50 ranges of Photofrin-PDT and sinoporphyrin sodium-PDT towards human cancer cells were 0.3 μg/mL to 5.5 μg/mL and 0.1 μg/mL to 0.8 μg/mL under the same irradiation conditions, respectively. The IC50 values of Photofrin-PDT and sinoporphyrin sodium-PDT towards human skin cells, HaCaT, were 10 μg/mL and 1.0 μg/mL, respectively. Esophagus carcinoma and hepatoma xenograft models were established to evaluate the in vivo antineoplastic efficacy. A control group, Photofrin-PDT group (20 mg/kg) and sinoporphyrin sodium group at three doses, 0.5 mg/kg, 1 mg/kg and 2 mg/kg, were set. Mice were injected with photosensitizers 24 h before 60 J 630 nm laser irradiation. The tumor weight inhibition ratio of 2 mg/kg sinoporphyrin sodium-PDT reached approximately 90%. Besides, the tumor growths were significantly slowed down by 2 mg/kg sinoporphyrin sodium-PDT, which was equivalent to 20 mg/kg Photofrin-PDT. In sum, sinoporphyrin sodium-PDT showed great anticancer efficacy and with a smaller dose compared with Photofrin. Further investigations are warranted.


Introduction
Photodynamic therapy (PDT) has been considered as a promising antineoplastic therapy since the 1990s. This new therapy is based on the specialty of photosensitizers (PS), which can accumulate selectively in tumor tissues and generate free radicals upon laser irradiation at a specific wavelength [1]. Preference localization of the photosensitizer towards tumor tissues and irradiation with a precise laser delivery system guarantee the PDT's double selectiveness, which improves the antineoplastic efficacy while reducing the toxicity at the same time. In addition, PDT killing is based on free radicals attacking, which is non-specific, indicating that it could be utilized repeatedly without significant resistance being observed.
As early as 1988, the porphyrin dimer with an ether linkage has been synthesized from 2-(1-hydroxyethyl) deuteroporphyrin dimethyl ester and found to be an effective antineoplastic photosensitizer in the murine SMT-F tumor model [2]. Since then, more porphyrin dimers have been proved to be effective towards malignant cells in vivo and with reduced skin phototoxicity [3]. Porphyrin-based structure chemicals usually have five absorbance bands in their excitation spectrum.
Porphyrin-based structure chemicals usually have five absorbance bands in their excitation spectrum. The Soret bond is located at approximately 420 nm and four Q bonds are distributed from 500 nm to 700 nm. Upon illumination, the quantum yields of singlet oxygen are satisfactory in porphyrin derivatives. Sinoporphyrin sodium is a component of Photofrin, the first US Food and Drug Administration (FDA) approved photosensitizer in the world ( Figure 1). As a promising anticancer photosensitizer candidate, sinoporphyrin was first isolated from Photofrin and proved to be one of the most potent photoactive compounds ( Figure 2). Fang et al. designed the new synthesis route of this compound with high synthetic yield by using the protoporphyrin dimethyl ester as a raw material. The reaction product is separated and purified by a silica gel column. A satisfactory purity can be achieved at 98.5% [4,5]. Its great water solubility and reliable efficacy tested in a pilot screening study make it a good photosensitizer candidate for PDT research [6,7].  This article evaluates sinoporphyrin sodium-mediated photodynamic therapy (sinoporphyrin sodium-PDT) thoroughly via in vitro and in vivo models. Photofrin, the front-line agent in the PDT field, thereby was chosen as the positive control in our study. The clonogenic assay, cell proliferation assay (CPA) and tumor growth evaluation in athymic mice were utilized to evaluate the anticancer efficacy of sinoporphyrin sodium-PDT.

Clonogenic Assay
For the clonogenic assay, breast adenocarcinoma MCF-7, renal carcinoma Ketr 3, liver hepatoma HepG2 and large cell lung cancer H460 cell lines were explored at three dose levels of sinoporphyrin sodium, which were 0.5 μg/mL, 0.05 μg/mL, and 0.005 μg/mL, respectively, and received identical doses of light illumination ( Figure 3). Porphyrin-based structure chemicals usually have five absorbance bands in their excitation spectrum. The Soret bond is located at approximately 420 nm and four Q bonds are distributed from 500 nm to 700 nm. Upon illumination, the quantum yields of singlet oxygen are satisfactory in porphyrin derivatives. Sinoporphyrin sodium is a component of Photofrin, the first US Food and Drug Administration (FDA) approved photosensitizer in the world ( Figure 1). As a promising anticancer photosensitizer candidate, sinoporphyrin was first isolated from Photofrin and proved to be one of the most potent photoactive compounds ( Figure 2). Fang et al. designed the new synthesis route of this compound with high synthetic yield by using the protoporphyrin dimethyl ester as a raw material. The reaction product is separated and purified by a silica gel column. A satisfactory purity can be achieved at 98.5% [4,5]. Its great water solubility and reliable efficacy tested in a pilot screening study make it a good photosensitizer candidate for PDT research [6,7].  This article evaluates sinoporphyrin sodium-mediated photodynamic therapy (sinoporphyrin sodium-PDT) thoroughly via in vitro and in vivo models. Photofrin, the front-line agent in the PDT field, thereby was chosen as the positive control in our study. The clonogenic assay, cell proliferation assay (CPA) and tumor growth evaluation in athymic mice were utilized to evaluate the anticancer efficacy of sinoporphyrin sodium-PDT.

Clonogenic Assay
For the clonogenic assay, breast adenocarcinoma MCF-7, renal carcinoma Ketr 3, liver hepatoma HepG2 and large cell lung cancer H460 cell lines were explored at three dose levels of sinoporphyrin sodium, which were 0.5 μg/mL, 0.05 μg/mL, and 0.005 μg/mL, respectively, and received identical doses of light illumination (Figure 3).  This article evaluates sinoporphyrin sodium-mediated photodynamic therapy (sinoporphyrin sodium-PDT) thoroughly via in vitro and in vivo models. Photofrin, the front-line agent in the PDT field, thereby was chosen as the positive control in our study. The clonogenic assay, cell proliferation assay (CPA) and tumor growth evaluation in athymic mice were utilized to evaluate the anticancer efficacy of sinoporphyrin sodium-PDT.
The clonogenicity inhibition rates increased in a photosensitizer dose-dependent manner ( Table 1). The highest dose plus laser illumination could inhibit clone viabilities of all four tumor cell lines by 85% to 94%.

Cell Viability Assay
Eleven human cancer cell lines as well as normal human skin cells, human immortal keratinocyte cells, HaCaT, were explored in the cell viability assay. The IC50 values for both the positive control Photofrin and sinoporphyrin sodium were achieved for each cell line ( Table 2). For human cancer cell lines, the IC50 ranges of Photofrin and sinoporphyrin sodium were from 0.3 μg/mL to 5 μg/mL Control groups (10% FBS-containing complete medium); Sinoporphyrin sodium concentration: high groups (0.5 µg/mL), medium groups (0.05 µg/mL) and low groups (0.005 µg/mL). * p < 0.05, when compared with control groups.
The clonogenicity inhibition rates increased in a photosensitizer dose-dependent manner ( Table 1). The highest dose plus laser illumination could inhibit clone viabilities of all four tumor cell lines by 85% to 94%.

Cell Viability Assay
Eleven human cancer cell lines as well as normal human skin cells, human immortal keratinocyte cells, HaCaT, were explored in the cell viability assay. The IC 50 values for both the positive control Photofrin and sinoporphyrin sodium were achieved for each cell line (Table 2). For human cancer cell lines, the IC 50 ranges of Photofrin and sinoporphyrin sodium were from 0.3 µg/mL to 5 µg/mL and 0.1 µg/mL to 1 µg/mL, respectively. For human immortal keratinocyte cells, the IC 50 values of Photofrin ® and sinoporphyrin sodium were approximately 10.4 µg/mL and 1.4 µg/mL, respectively. Table 2. IC 50 values of Photofrin-PDT and sinoporphyrin sodium-PDT towards human cancer cell lines and immortal keratinocyte cells (X ± SD).

Human Esophagus Tumor CaEs-17 Xenograft Models
There were two aspects of the xenograft model experiments: the tumor weights and the tumor volumes. The tumor weights in all groups are shown in the bar graphs in Figure 4, which possess a clear sinoporphyrin sodium dose-dependent manner in the three treatment groups. The tumor weight inhibition ratios are plotted as the red full line in Figure 4. The 2 mg/kg sinoporphyrin sodium-PDT had a similar anticancer efficacy as 20 mg/kg Photofrin-PDT.

Human Esophagus Tumor CaEs-17 Xenograft Models
There were two aspects of the xenograft model experiments: the tumor weights and the tumor volumes. The tumor weights in all groups are shown in the bar graphs in Figure 4, which possess a clear sinoporphyrin sodium dose-dependent manner in the three treatment groups. The tumor weight inhibition ratios are plotted as the red full line in Figure 4. The 2 mg/kg sinoporphyrin sodium-PDT had a similar anticancer efficacy as 20 mg/kg Photofrin-PDT.  Table 3. The relative tumor volume (RTV) and ratio of RTVtreatment and RTVcontrol (T/C) were also calculated.  Table 3. The relative tumor volume (RTV) and ratio of RTV treatment and RTV control (T/C) were also calculated.

Human Hepatoma HepG2
The tumor weights in all groups as well as the inhibition rates are plotted as bar graphs and red full lines in Figure 5. The similar inhibition trends shown in CaEs-17 xenografts were also seen in Hepatoma HepG2 xenograft experiments.

Human Hepatoma HepG2
The tumor weights in all groups as well as the inhibition rates are plotted as bar graphs and red full lines in Figure 5. The similar inhibition trends shown in CaEs-17 xenografts were also seen in Hepatoma HepG2 xenograft experiments.  Table 4.   Table 4.

Discussion
Photodynamic therapy in oncology has been a heated topic in the PDT field, since the mainstream regimens could not fully live up to the high expectations of the patients. The dual selectivity achieved by the preferential photosensitizer uptake of malignant cells and the targeted laser illumination enable PDT to be utilized for both curative and alleviative purposes. The comparatively transient and tolerable adverse effects profiles make this treatment a good alternative regimen that can be considered when standard treatment fails or cannot be tolerated.
Preclinical investigation is crucially important in the oncology field given the high failure rates and huge investments into subsequent clinical trials. The traditional clonogenic assay, the MTT assay as well as xenograft models are still the most used screening tests in evaluating anticancer candidates [8].
The clonogenic assays examined the effect of sinoporphyrin sodium-PDT towards the clonogenicity ability of MCF-7, H460, HepG2 and ketr 3 cell lines at different dose levels and with identical laser illumination. Decreased clone survivals were observed in all treatment groups. At the 0.5 µg/mL dose level, few clones were observed in all cell lines. At the 0.05 µg/mL dose level, the clonogenic ability varied within the four cell lines, ranging from 50% to 80%.
In the cell viability assay, we tested 11 human cells lines, which included hepatoma, colorectal cancer, breast cancer, lung cancer, melanoma, ovarian cancer, neuronal glioblastoma, renal carcinoma as well as gastric cancer. These cancer types are among the most fatal diseases throughout the world, with high incidence and high mortality at the same time. According to the results, the cancer cells showed different levels of responsiveness to the two PDT regimens. Generally, colorectal cancer and breast cancer were more responsive to both Photofrin-PDT and sinoporphyrin sodium-PDT. The results indicated that Photofrin-PDT exhibited great antineoplastic efficacy towards multiple cancer cell lines with IC 50 values ranging from 0.3 µg/mL to 5.5 µg/mL. Meanwhile, the IC 50 values of sinoporphyrin sodium-PDT were 0.1 µg/mL to 0.8 µg/mL. For normal cells, the killing efficacy of PDT mediated by both photosensitizers was decreased given that the IC 50 values of Photofrin-PDT and sinoporphyrin sodium-PDT were 10 times higher towards the human immortal keratinocyte HaCaT cell line.
In the esophagus tumor and hepatoma xenograft experiments, the sinoporphyrin sodium's dose-dependent killing manner was clearly observed. In terms of tumor volume, the PDT treatment obviously slowed down the tumor growth in both models. Interestingly, in the 2 mg/kg sinoporphyrin sodium-PDT and 20 mg/kg Photofrin-PDT groups, the tumor shrinkages were also observed, which indicated that PDT treatment not only prevents tumor proliferation, but also exerts effects on the tumor mass that had been inoculated before the PDT process. It was highly possible that other mechanisms, such as anti-vasculature as well as autophagy pathways, may exist in the experiments [9].
Further, the phototoxicities observed in the in vivo experiments were also of importance. The skin toxicity had not been quantitatively evaluated in this article. In our experiments, only acute skin toxicity was observed, which appeared the next day after PDT irradiation at the illumination site and neighboring areas of mice with the presentation of swelling and edema. The whitened color indicated impaired blood vasculature which lasted for the following three to four days. The incrustation took place approximately at days 4-5 after irradiation. The color of the scab in the Photofrin group was yellow and that of the sinoporphyrin sodium group was white. The duration of swelling varied with different treatment groups. Swelling of the illumination spots continued for seven to 10 days for animals in the Photofrin and high sinoporphyrin sodium dose group. After the swelling retreated, the tumor sites in the treatment groups tended to be flat and began to shrink. The local injuries after PDT irradiation were described in one study which showed that severe hypoxia was the main physiological change in the tissues [10]. This observation indicated that PDT at the high dose level may cause acute and severe phototoxicity. No persistent skin toxicity has been observed under indoor light illumination in our experiments.
Many chemical structures have been developed as photosensitizer candidates, such as porphyrins, chlorins and phthalocyanines. The phthalocyanine and chlorin compounds usually have longer excitation wavelengths and stronger absorbance ensuring deeper penetration into the tissue, which may further expand the cancer profiles that PDT could treat. However, for porphyrin derivatives, the comparatively shorter excitation wavelength and thereby the superficial illumination area are now difficult to improve. However, given their good safety profile, porphyrins are still the most used photosensitizers in clinical settings.
Researchers have been investigating ways to improve porphyrins-PDT, such as with the development of optical fibers coupled with PDT as well as structure modification of the photosensitizers to increase their targeting efficacy [11]. Besides, designing reasonable PDT regimens based on pharmacokinetic parameters of photosensitizers could also achieve this goal. Xiong W. et al. [12] conducted a comparative study of two kinds of repeated sinoporphyrin sodium-PDT strategies using the breast cancer xenograft model. In regimen 1, sinoporphyrin sodium was only injected one time and then the mice were exposed to the 50 J/cm 2 laser 24 h, 30 h and 36 h after photosensitizer administration. In regimen 2, sinoporphyrin sodium was injected three times and mice received the 50 J/cm 2 laser 24 h after each injection. The tumor volume inhibition ratios were 85.8% ± 7.6% and 65.7% ± 8.6% for regimen 1 and 2, respectively. Also, Kessel D. [13] explored the possibility of a two-sensitizer sequential PDT protocol to enhance the efficacy of photo-killing. The possible mechanism was that the photo-damage caused by a low-level lysosome-targeted photosensitizer could promote the apoptotic responses caused by a subsequent mitochondria-targeted photosensitizer. These two methods were new but reasonable ways to optimize PDT treatment strategies based on photosensitizers' pharmacodynamic and pharmacokinetic profiles.
Wang H. et al. [14] explored the cellular uptake of sinoporphyrin sodium in ECA-109 cells and found it was mainly located in the mitochondria. Given the short existence time and thereby the short diffusion distance of reactive oxygen species (ROS), the cytotoxicity mechanism of sinoporphyrin sodium-mediated PDT may be induced by injury to the mitochondria. Injury to the mitochondria may initiate apoptotic responses, including cytochrome c release into the cytoplasm and caspase protein activation [15]. Lv W. et al. [16] found that the efficacy of a mitochondria-targeted photosensitizer-mediated PDT may be improved under a hypoxic environment, which may be another way to improve sinoporphyrin sodium-PDT.
There were some limitations of this research. First, the traditional subcutaneous implanted xenograft models we used in the sinoporphyrin sodium-PDT evaluation may be different from the natural pathogenesis of esophagus and hepatocellular carcinoma compared with orthotopic transplantation models and transgenic or knock-out animals [17]. We finally chose subcutaneously inoculated models (s.c. models), which usually do not have lymphatic and distal metastasis, given that PDT has been mostly utilized in premalignant and early stages of cancer therapy. So, it should be noted that s.c. inoculated models were used in this study when the results are to be interpreted and compared. Second, the point-to-point laser illumination mode avoided many possible adverse effects that could take place in the clinic, such as bleeding, esophageal fistula, etc., introduced by an endoscopic fiber-coupled PDT process [18]. Third, we did not explore the possible different laser parameters that could further improve the efficacy.

Clonogenic Assay
The sinoporphyrin sodium (bis[1-[6,7-bis[2-(sodium carbonate) ethyl] 1,3,5,8-tetra-methyl-2-vinylporphin-4-yl]-ethyl] ether, Qinglong High-Tech Co., Ltd., Yichun, China, Figure 1) stock solution was prepared in 0.9% NaCl, which at a concentration of 2 mg/mL. Further dilution was performed in 10% FCS (Zhejiang Tianhang Biotechnology Co., Ltd., Hangzhou, China) RPMI1640 media (Gibco ® , Grand Island, NY, USA). Three working solution was achieved at the final concentration of 0.5 µg/mL, 0.05 µg/mL, 0.005 µg/mL, respectively. Four groups were set in clonogenic assay (1) control group; (2) Low dose group with 0.005 µg/mL sinoporphyrin sodium; (3) Medium dose group with 0.05 µg/mL sinoporphyrin sodium; and (4) High dose group with 0.5 µg/mL sinoporphyrin sodium. All groups were done in triplicate. MCF-7, ketr 3, HepG2 and H460 cells were seeded in six-well plates (Corning, NY, USA) at density of 200 cells per well and incubated under condition of 37 • C and 5% CO 2 (Thermo, Waltham, MA, USA) for 24 h. The cells were rinsed with plain medium and then dosed with sinoporphyrin sodium at 0.005 µg/mL, 0.05 µg/mL and 0.5 µg/mL. The cells in control well were incubated with complete medium. Four hours after dosing, the medium in all plates were replaced with plain media and were about to undergo PDT process. The 630 nm laser (Xingda Photoelectric Medical Instrument Co., Guilin, China) was applied to incubation well at fluence rate of 30 mW/cm 2 and lasted for 180 s well by well. The total illumination power was 5.4 Joules per well. Plain medium in all wells were replaced with 10% FBS-containing complete medium after PDT process. And, all plates were returned to incubator for the next seven days. After the incubation period, the medium were discarded and cells were rinse twice with 2 mL PBS (Hyclone, Logan, UT, USA). The cells were fixed with 2 mL methanol (Chemical Reagent Beijing Co., Ltd., Beijing, China) for 5 min. This process was repeated twice. Then, the cells were stained with 2 mL 0.1% violet solution (Chemical Reagent Beijing Co., Ltd., Beijing, China) for 5 min. The staining solution was washed away by pure water. After fully drying, the clones in each plate were counted by naked eyes. The clone numbers in triplicate were averaged and compared with control group. The Clonogenicity efficiency (CE%, Equation (1)) and clonogenicity inhibition rate (CI%, Equation (2)) were calculated. where: (1) CE% stands for clonogenicity efficiency percentage (2) CI% stands for clonogenicity inhibition percentage (3) N was clone number of each group. N 0 was cells seeded in well at day 1, which was 200 in this case. (4) N treatment was clone number of each treatment group. N control was clone number of control group.

Monolayer PDT Efficacy via MTT Assay
Monolayer response to Sinoporphyrin sodium-PDT and Photofrin-PDT were measured using the MTT colorimetric assay. Human cancer cell lines of U251, bel7402, MCF-7, BGC-823, ketr 3, A2780, HCT-8, HCT-116, HepG2, A375 as well as normal human cell HaCaT were included in MTT assays. The figuration of 96-well plate was divided into positive control part and sinoporphyrin sodium part ( Figure 6).   Single cell solution were made and seeded in 96-well plates (Corning, NY, USA) at density of 1000 cells per well 24 h prior to experiment. Triplicates were done for each cell line. The 96-well plates were incubated under condition of 37 • C and 5% CO 2 (Thermo, Waltham, MA, USA). The cells were dosed with different concentration of Photofrin ® (AXCAN PHARMA INC, Lot OK 102, Mont Saint Hilaire, QC, Canada) or Sinoporphyrin sodium after PBS rinse. The concentration set in Photofrin ® part were 100 µg/mL, 10 µg/mL, 1 µg/mL, 0.1 µg/mL and 0.01 µg/mL. The concentration in sinoporphyrin sodium part was 10 µg/mL, 1 µg/mL, 0.1 µg/mL, 0.01 µg/mL and 0.001 µg/mL. The cells were co-incubated with two different photosensitizers for subsequent 4 h. Before PDT process, the photosensitizer-containing media were replaced by plain media in all wells. The 630 nm laser (Xingda Photoelectric Medical Instrument Co., Guilin, China) was applied to each plate at fluence rate of 30 mw/cm 2 with illumination diameter of 80 mm. The illumination lasted for 180 s for each plate. Thus, the total laser power was 1.6 Joules per well. The photosensitizer containing medium was replaced with plain media and the plates were incubated for following 72 h. After three-day incubation, cells were incubated with 150 µL 0. The inhibition rates of different dose level of two photosensitizers were calculated (Equation (3)). The IC 50 values were achieved by plotting the values against the concentration.

Establishing Xenograft Models
The xenograft-bearing nude mice were established to evaluate the efficacy of sinoporphyrin sodium-PDT. Xenografts of human esophagus carcinoma CaEs-17 and hepatoma HepG2 were kindly donated by Professor Zhaodi Fu (Institute of Materia Medica, CAMS & PUMC). The xenografts of esophagus carcinoma CaEs-17 and hepatoma HepG2 were thawed and passaged in nude mice for two passages. Tumor mass with volume of approximately 1 mm 3 were inoculated in the right forelimbs of the SPF level Balb/C-nu/nu female mice (Vital River Laboratories, Beijing, China), weighing 18 to 20 g. After inoculation, mice were bred in SPF level animal hood under controlled temperature and humidity. Food and water were provided ad libitum. The major and minor diameters of implants were measured by caliper every two days until the tumor volume were about 100 mm 3 (Equation (4)).
Tumor Volume (TV) = D length × D width

Antineoplastic Efficacy Evaluation via Xenograft Models
Five groups, including control group (solvent control), positive control (Photofrin ® 20 mg·kg −1 ) and sinoporphyrin sodium low dose group (0.5 mg·kg −1 ), medium dose group (1 mg·kg −1 ) and high dose group (2 mg·kg −1 ) were set in the experiments. The photosensitizers were administered through tail vein when the tumor volumes of xenografts in all groups reached approximately 100 mm 3 . Except control group, all treatment groups received identical laser illumination at 127.7 mW/cm 2 24 h after photosensitizer administration. The laser beam was aimed at tumor inoculation area within a diameter of 10 mm and lasted for 600 s. The total laser power was 60 Joules per animal. Then all mice were kept under SPF level animal facility for next 21 days, during which time, the tumor sizes were monitored by caliper measurement. At the end of recovery period, animal body and tumor weight were measured and animals were sacrificed. The tumor were then dissected out and weighed. The relative tumor volume (RTV, Equation (5)) values and T/C values (Equation (6)) were calculated and compared among groups. Both xenografts experiments were conducted twice to ensure the accuracy.
Relative tumor volume (RTV) = V t V 0 , T/C (%) = RTV treatment RTV control × 100% where: (1) V t stands for tumor volume at different days in the treatment periods.
(2) V 0 stands for the tumor volume at the day 1 of the experiment.
(3) RTV treatment stands for RTV values of different treatment groups.
(4) RTV control stands for RTV value of control group.
The experiment design and procedures involving laboratory animals were approved by Institutional Animal Care and Use Committee (IACUC) in Institute of Materia Medica, CAMS & PUMC. All animal manipulation was complied with the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) guidelines for animal welfare.

Data Process and Statistical Study
All numerical data were expressed as mean ± SD and were statistically compared by One-Way ANOVA using software SPSS 16.0 (Chicago, IL, USA). The p values < 0.5 was accepted as significance.

Conclusions
The present study showed that sinoporphyrin sodium-PDT possessed a great antineoplastic effect towards both monolayer human cancer cells and xenograft models compared with Photofrin. Given the lesser dose used and the safety profile observed in our studies, sinoporphyrin sodium was proved to be a good photosensitizer candidate for use in the oncology field. Further studies are warranted.