Chemical Profile, Antioxidant and Antibacterial Activities of Achillea moschata Wulfen, an Endemic Species from the Alps

Aerial parts of Achillea moschata Wulfen (Asteraceae) growing wild in the Italian Rhaetian Alps were investigated to describe, for the first time, their phenolic content, as well as to characterize the essential oil. Inspection of the metabolic profile combining HPLC-DAD and ESI-MS/MS data showed that the methanol extract contained glycosylated flavonoids with luteolin and apigenin as the main aglycones. Among them, the major compound was 7-O-glucosyl apigenin. Caffeoyl derivates were other phenolics identified. The essential oil obtained by steam distillation and investigated by GC/FID and GC/MS showed camphor, 1,8-cineole, and bornylacetate as the main constituents. The antioxidant capacity of three different extracts with increasing polarity and of the essential oil was evaluated by employing ABTS·+ and DPPH· radical scavenging assays. The methanolic extract was the only significantly effective sample against both synthetic radicals. All samples were also tested against Gram-positive (Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus) and Gram-negative (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa) bacterial species using the disk diffusion assay. The non-polar extracts (dichloromethane and petroleum ether) and the essential oil possessed a broad spectrum of antimicrobial activity expressed according to inhibition zone diameter (8–24 mm).


Introduction
The genus Achillea (Asteraceae) includes about 130 flowering and perennial species worldwide-mostly in Europe and temperate areas of Asia-but also in North America and North Africa [1,2]. Achillea moschata Wulfen is one of the 23 species recognized in Italy, where it grows on siliceous rocks, screes and stony pastures, along the Alps from 1800 m up to 3400 m a.s.l. [3]. It is a Infusion (to drink, to make compresses); Liqueur; Ointment (to rub) [4][5][6][7][8][9][10][11] Veterinary medicine Digestion and skin inflammations (cows and calves) Infusion (to drink, to make compresses) [8][9][10][11] Food Flavouring of typical dishes, soups, desserts, grappa, liqueurs, spicing cheese [10,11] Despite its long tradition of collection and use, A. moschata has been poorly studied to date. Scientific reports on this plant are very few and essentially limited to germination, altitudinal adaptation and chemosystematics [12][13][14]. The oil volatile composition was characterized more than 20 years ago [15,16]. Therefore, the aim of the present work was the re-investigation of the essential oil composition and the characterization, for the first time, of the phenolic constituents in the aerial parts. In vitro antioxidant and antibacterial activities of both the essential oil and of plant extracts were also measured.

Characterization of the Essential Oil
The essential oil content of A. moschata obtained by steam distillation from the dried aerial parts of the plant was quantified as 0.81%, which was then analyzed by GC/FID and GC/MS. The constituents are reported in Table 2, wherein they are listed in ascending order of their retention indices (RIs) on a DB-5 column. A total of 40 components were identified, representing 94.36% of the oil.
Several compounds were detected belonging to different chemical classes, with monoterpenes and sesquiterpenes identified as the most abundant constituents. A series of unknown compounds were tentatively identified by comparing their MS spectra with those of known compounds.
Monoterpenes made up 81.80% of the total oil, with oxygenated monoterpenes and monoterpene hydrocarbons detected at 68.44% and 23.36%, respectively. Sesquiterpenes were found in lesser amounts than monoterpenes, and represented 7.25% of the sample. Oxigenated sesquiterpenes were the most abundant of this class of constituents, representing 5.62% of the total oil. Camphor was found to be the major constituent (27.16% of the oil,) followed by 1,8-cineole (10.69%) and bornylacetate (6.21%).
Other constituents detected in relatively high amounts were the monoterpenes: cisand trans-thujone (4.78 and 4.82%, respectively), borneol (4.77%), cis-sabinene hydrate (4.28%) and camphene (3.82%). Our data were in agreement with those available from the literature [15,16] although some differences in the quantitative composition of the oil were observed, likely due to the different origin of the plant material under study.  [17]. RI b : Retention Indices calculated by GC/MS using n-alkane series (from C 6 to C 32 ) under the same analytical conditions as for the samples.

Qualitative Analysis of the Methanol Extract
The phenolic compounds were characterized according to their UV, HPLC, retention times, mass spectra, and comparison with authentic standards when available. Table 3 shows the chemical composition of the methanolic extract of A. moschata. TLC analyses showed several spots of different colors that were indicative of a complex metabolic profile. A typical intense fluorescence under UV-365 nm was observed immediately after spraying with NP/PEG reagent. As reported in literature [18], the fluorescence behavior is structure-dependent. In particular, flavonols such as glycosides of kaempferol and isorhamnetin give yellow-green fluorescence; flavones such as glycosides of luteolin give an orange color, while glycosides of apigenin produce yellow-green fluorescence and phenolic carboxylic acids appear as intense light blue zones. Taking into account the above reported considerations, we concluded that flavonols and phenolic acids were present in the extract flavones. TLC analyses of the two polar extracts obtained by sonification and Soxhlet (see MM) showed a superimposable profile.
These preliminary data were confirmed by ESI-MS/MS analyses. Spectra of constituents of A. moschata extract showed different fragmentation patterns, suggesting the presence of flavonoids and phenolic acids. In particular, flavonoids had characteristic fragmentation patterns as follows: In addition, it was possible to confirm a pattern of fragmentation typical of caffeic acid derivatives: fragments ions at m/z 173 or 191 consistent with quinic acid moieties of mono-acyl or di-acyl chlorogenic acids, and fragment ions at m/z 179 characteristic of a caffeoyl substituent.
This information was supported by UV-data. The UV spectra showed typical behavior of flavonoids and caffeic acid derivatives. The first metabolites exhibited two major absorption bands: band I absorption in the 330-350 nm range due to the B-ring cynnamoyl system with weak absorption (sh) around 299 nm; band II in the range 240-280 nm due to A-ring benzoyl system for flavone derivatives while flavonols showed band II in the range 250-270 nm and band I in the range 350-390 nm.
Compounds with absorption ranges at 325.7-329.3 nm and 246.1-250.0 nm plus a diagnostic sharp shoulder at 290-300 nm were unequivocally identified as chlorogenic acids.
Two groups of major flavones were detected: a group with a band II having two absorption maxima or one maximum with a shoulder (in the range of 250-265 nm)-similar to luteolin (3 1 ,4 1 ,5, 7-tetrahydroxyflavone) derivatives-and another group with a maximum absorption corresponding to the apigenin (4 1 ,5,7-trihydroxyflavone) derivatives. Moreover, the UV spectra of the extracts showed the presence of flavonols. TLC analyses showed several spots of different colors that were indicative of a complex metabolic profile. A typical intense fluorescence under UV-365 nm was observed immediately after spraying with NP/PEG reagent. As reported in literature [18], the fluorescence behavior is structuredependent. In particular, flavonols such as glycosides of kaempferol and isorhamnetin give yellowgreen fluorescence; flavones such as glycosides of luteolin give an orange color, while glycosides of apigenin produce yellow-green fluorescence and phenolic carboxylic acids appear as intense light blue zones. Taking into account the above reported considerations, we concluded that flavonols and phenolic acids were present in the extract flavones. TLC analyses of the two polar extracts obtained by sonification and Soxhlet (see MM) showed a superimposable profile.
These preliminary data were confirmed by ESI-MS/MS analyses. Spectra of constituents of A. moschata extract showed different fragmentation patterns, suggesting the presence of flavonoids and phenolic acids. In particular, flavonoids had characteristic fragmentation patterns as follows: In addition, it was possible to confirm a pattern of fragmentation typical of caffeic acid derivatives: fragments ions at m/z 173 or 191 consistent with quinic acid moieties of mono-acyl or di-acyl chlorogenic acids, and fragment ions at m/z 179 characteristic of a caffeoyl substituent.
This information was supported by UV-data. The UV spectra showed typical behavior of flavonoids and caffeic acid derivatives. The first metabolites exhibited two major absorption bands: band I absorption in the 330-350 nm range due to the B-ring cynnamoyl system with weak absorption (sh) around 299 nm; band II in the range 240-280 nm due to A-ring benzoyl system for flavone derivatives while flavonols showed band II in the range 250-270 nm and band I in the range 350-390 nm.
Compounds with absorption ranges at 325.7-329.3 nm and 246.1-250.0 nm plus a diagnostic sharp shoulder at 290-300 nm were unequivocally identified as chlorogenic acids.
Two groups of major flavones were detected: a group with a band II having two absorption maxima or one maximum with a shoulder (in the range of 250-265 nm)-similar to luteolin (3′,4′,5, 7-tetrahydroxyflavone) derivatives-and another group with a maximum absorption corresponding to the apigenin (4′,5,7-trihydroxyflavone) derivatives. Moreover, the UV spectra of the extracts showed the presence of flavonols.  The sample showed the presence of a prevalent compound (peak 3, Figure 1) with a UV spectrum typical of the apigenin derivatives. This compound was also isolated by HPLC and analyzed by ESI-MS/MS. The MS spectra showed a pseudomolecular ion at m/z 431 and the MS 2 revealed the loss of one hexose unit and the appearance of the aglycone fragment at m/z 269 (100) [(M´H)´162]´(apigenin), due to the cleavage at the glycosidic-O-linkage. The metabolite was identified as 7-O-β-glucosyl apigenin. This result was confirmed by comparison between the retention time of the samples and the reference compound.
Peak 1 showed a UV spectra characterized by absorption at 250.0 nm, 325.7 nm and a shoulder peak at 299.0 nm. This UV spectrum is typical of phenolic compounds. The MS spectrum of this compound was characterized by a marker fragment at m/z 191 due to loss of a caffeic moiety (162 Da). Peak 1 was unambiguously identified as 5-O-caffeoylquinic acid by comparing its retention time with that of a commercial standard.
The HPLC profile of A. moschata presented another phenolic compound such as the peak 4 ([M´H]´at m/z 515) corresponding to a dicaffeoylquinic acid. This compound was identified as 4,5-O-dicaffeoylquinic acid according to the retention time of the reference standard and the MS 2 spectra. MS 2 fragmentation was identical to the one previously reported by Clifford et al. [19] and Carbonara et al. [20].
Other flavones were identified in the extract, most of them associated to apigenin and luteolin derivatives based on their UV spectra (λ max 330-350 nm) and MS 2 fragmentation pattern. Luteolin-7-O-glucoside (peak 2) was positively identified according to the retention time, mass, and UV spectra similar to that of reference compound.
In addition, the extract was characterized by the presence of free aglycones. Peak 6 ([M´H]´at m/z 285) isolated by HPLC and analyzed by ESI-MS/MS analysis was identified as luteolin; peak 7 ([M´H]´at m/z 269) was identified as apigenin based on the retention time, mass, and UV spectra compared with a reference compound.
The remaining phenolic compounds in the extract corresponded to flavonols derivatives, most of them derived from kaempferol (λ max around 354 nm and MS 2 fragment at m/z 285) and isorhamnetin (λ max 349 band I and 255 nm band II and MS 2 fragment at m/z 315). The UV absorptions ( Table 3)
The chemical composition of A. moschata, shown for the first time in this study, confirmed the trend that was also reported by previous investigations on chemical constituents of the genus Achillea identifying phenolics among the most abundant compound classes [21]. In particular, flavonoids as flavones and flavonols and their derivatives are present, in addition to hydroxycinnamic acids [22]. A. moschata, like other Achillea species, had apigenin and luteolin as free flavonoid aglycones and, in some cases, also their monoglycosides. However, many differences were present, both in relation to the type and to the amount of compounds [21][22][23][24][25]. For example, in comparison with A. millefolium, the unique species present in the European Pharmacopeia, A. moschata showed a different phenolic profile of the methanolic extract. Flavonoids were the major phenolic constituents in A. moschata with 7-O-β-glucosyl apigenin being the most abundant one, which differs from A. millefolium in which phenolic acids are the major metabolites. Cis and trans 3.5-O-dicaffeoylquinic acids predominated in A. millefolium [26,27], while 5-O-caffeoylquinic acid in A. moschata.
Therefore, from a phytochemical point of view, several Achillea species were investigated and a wide diversity was found. With regards to the phenolic compounds, the reason for such variations may be explained by the role that they play as protective agents against oxidative stress related to climatic conditions and geographical distribution. It is known that type, content and proportion of the bioactive substances synthesized by the plants may vary depending on ecological factors including the annual average precipitation, temperature, frost-free period, soil pH, and organic matter [28].

Antioxidant Activity
The antioxidant activity of three extracts with increasing polarity-petroleum ether (PET), dichloromethane (DCM), methanol (MeOH)-and of the essential oil (EO) from A. moschata was assessed by employing ABTS¨+ (2,2 1 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and DPPH( 2,2-diphenyl-picryl hydrazyl) radical scavenging assays. A good correlation between the two methods was observed and the results are reported in Table 5. Among the extracts, the only sample showing values useful to calculate IC 50 was the MeOH extract. Its scavenging ability against the DPPH stable radical was higher than that of the reference compound, i.e., quercetin, in agreement with previous data from another population of A. moschata [29]. PET and DCM extracts had values of DPPH-scavenging lower than 50% (Table 5). Similarly, in the ABTS assay, the MeOH extract exhibited a high degree of activity, 6-and 10-fold stronger than DCM and PET extracts, respectively ( Table 5). The negligible radical inhibition of the latter extracts was due to the different solvent polarity, which influences the ability to dissolve the antioxidant compounds, as widely reported [30]. The EO was able to scavenge DPPH¨free radical less effectively than the MeOH extract, though with IC 50 values still significant. Instead, the EO antiradical capacity was weak when it was tested toward ABTS¨+ (Table 5). There are no previous data on the antioxidant activity of A. moschata essential oil. On the contrary, there are evidences to support the possible relationship between antioxidant activity and cytotoxicity. During the latest 20 years, the mechanism by which continued oxidative stress can lead to chronic inflammation-which could in turn mediate various diseases including cancer-was revealed [31]. Moreover, experimental studies (both in vitro and in vivo), demonstrated the role of plant natural compounds on growth inhibition of cancer cells [31][32][33][34]. Cytotoxic effects were also ascribed to the phenolics whose biological functions are, however, related to their structure. Compounds such as apigenin and luteolin, present in A. moschata, exert significant action towards specific cancer cells, while other compounds such as 7-O-β-glucosyl apigenin, the most abundant, were not investigated from this point of view [34,35]. Therefore, A. moschata could be an important cytotoxic species, also considering the strong antioxidant capacity of its methanol extract. Until now, several Achillea species with high antioxidant potential were tested in vitro against different tumor cell lines. They showed variable results depending on the metabolic profile of the crude extract or of the essential oil and their concentration, as well as the type of tumor cell line [22,[36][37][38].

Antimicrobial Activity
The MeOH, DCM, and PET extracts from the aerial parts and the distilled EO from A. moschata were assayed for their in vitro antimicrobial properties using disk diffusion method [39]. All samples were tested against strains having a particular significance for pathogenesis, drug-resistance, and nosocomial outbreaks [40,41]. Three Gram-positive (Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus) and three Gram-negative (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa) that grow aerobically have been chosen. Moreover, we have also considered that the herb A. moschata is used in several remedies such as gastrointestinal disorders, skin inflammations, urinary tract inflammations, infectious diseases (see Table 1) that can be caused by these bacteria. For instance, the food-borne pathogen B. cereus or bacteria that are often isolated from patients with urinary tract infections-E. faecalis, E. coli, P. mirabilis, P. aeruginosa-and the multidrug-resistant S. aureus (MRSA) which causes invasive infections. The growth inhibition diameters of the tested samples are displayed in Table 6. In contrast of the results of the antiradical capacity, the antimicrobial activity of the extracts was inversely proportional to their increasing polarity of the solvent used for their extraction. The MeOH extract showed a low inhibitory activity and only against Bacillus cereus (8 mm) and Staphylococcus aureus (8 mm). DCM extract was found to be active against five out of six tested bacteria (inactive against Escherichia coli), with inhibition diameters ranging from 8 to 15 mm. PET extract proved to be effective against all tested organisms, with inhibition diameters ranging from 8 to 15 mm.
EO demonstrated a broad spectrum of antimicrobial activity against the tested strains, in some cases greater or similar to the conventional antibiotics (erythromycin and ceftazidime). B. cereus was particularly susceptible, with an inhibition zone of 24 mm, followed by S. aureus (18 mm). The susceptibility of the other test bacteria was less pronounced, but still significant (10-13 mm). Lastly, EO showed no activity against Enterococcus faecalis which turned out to the most resistant among the tested microorganisms.
Disks only impregnated with extraction solvents were used as negative control and showed no inhibition of bacterial growth. The ceftazidime reference disks showed inhibition diameters ranging from 17 to 23 mm (E. coli, Proteus mirabilis, and Pseudomonas aeruginosa). The erythromycin reference disks showed inhibition diameters ranging from 16 to 20 mm (B. cereus and S. aureus), while no inhibitory effect was observed against E. faecalis.
No previous data about the antimicrobial activity of A. moschata have been reported until now. However, some studies were carried out on other Achillea species. Their polar extracts, in agreement with our results, mostly showed weak or no inhibitory effect against the test microrganisms [42][43][44]. These findings were also in accordance with those of Sökmen and co-authors [45] who observed no antimicrobial activity in the water soluble part of methanol extract from A. biebersteinii, while water-insoluble (CHCl 3 ) part characterized by less polar compounds was found to have moderate activity against some tested bacteria. The only sample which was strongly active derived from a plant extract obtained by mixing ether, hexane and MeOH (1:1:1) [46]. The antibacterial properties of EO were probably due to their high content of camphor and 1,8-cineol, whose noticeable antimicrobial potential is known [21] as well as that of borneol [47].
A more detailed comparison of the results is difficult due to the fact that the same type of extract and essential oil may vary in efficacy if obtained from the same species collected in different locations. In fact, soil, altitude, and other environmental factors can influence the type and the levels of antimicrobials synthesized by the plant [48].
Diethyl ether and a series of authentic reference compounds used for identification of the essential oil components were also purchased from Sigma-Aldrich (Milan, Italy).

Plant Material
The aerial parts of A. moschata were collected at the blossom period during the summer 2013, at 2400 m above sea level in Valle dei Forni (Sondrio province, Italy), on the Rhaetian Alps. A voucher specimen (No. AMSHT 103) was deposited at the Department of Agricultural and Environmental Sciences of the Milan State University (Milan, Italy), after its identification according to Flora d'Italia [3].

Isolation of Essential Oil
The air-dried aerial parts (24 g) of the sample were subjected to steam-distillation for 1 h in a Clevenger-type apparatus. The distillate was saturated with NaCl, extracted with freshly distilled Et 2 O (3ˆ100 mL), dried over anhydrous Na 2 SO 4 and concentrated with a rotary evaporator at 30˝C to give a pale-blue yellow oil with a yield of 0.81%. The oil was diluted with Et 2 O and then used for gaschromatographic analyses.

Gas Chromatography-Flame Ionization Detector (GC/FID)
GC/FID analyses were carried out using a Perkin Elmer model 8500 GC equipped with a 30 mˆ0.32 mm i.d. Elite-5MS capillary column (0.32 µm film thickness). Samples (0.5 µL) were injected in the "split" mode (1:30) with a column temperature programme of 40˝C for 5 min, then increased to 260˝C at 4˝C/min and finally held at this temperature for 10 min. The injector and the detector were set at 250 and 300˝C, respectively; the carrier gas was He with a head pressure of 12.0 psi.

Gas Chromatography-Mass Spectrometry (GC/MS)
GC/MS analyses were carried out using a Perkin Elmer Clarus 500 GC equipped with a Clarus 500 mass spectrometer using the same capillary column and chromatographic conditions as was used for GC/FID analysis. The mass spectra were acquired over 40-500 amu range at 1 scan/s with ionizing electron energy of 70 eV and the ion source set at 200˝C. The transfer line was set at 280˝C, while the carrier gas was He at 1.0 mL/min.

Identification and Quantitation of the Essential Oil Components
The identification of the volatile oil components was performed by their Retention Indices (marked as RI in Table 2) and their mass spectra, by comparison with a NIST database mass spectral library [49], as well as with literature data [17,50].
Authentic reference compounds purchased from Sigma-Aldrich were also used. Retention indices were calculated using a n-alkane series (C 6 -C 32 ) under the same GC conditions as for the samples. The relative amounts of individual components of the essential oil were expressed as percent peak area relative to total peak area from the GC/FID analyses of the whole extracts.

Extraction and Isolation
Hydroalcholic extract was obtained from dry aerial parts of A. moschata plants. The sample (4 g) was extracted twice by sonicator with methanol 80% (150 mLˆ2) for 30 min, and subsequently filtered through a filter paper. The combined hydroalcholic extracts were evaporated at 30˝C (rotary evaporator) to dryness. The dry extract was re-dissolved in methanol (final concentration 30 mg/mL) for phenolic compound identification and quantification. In addition, the sample was extracted with Soxhlet in methanol (9 gˆ3 h). To obtain non-polar extracts, aerial parts were also defatted with petroleum ether and successively extracted with dichloromethane (Soxhlet apparatus), then concentrated under reduced pressure (rotary evaporator) to dryness and stored at 4˝C until analyses.

HPLC
The HPLC analysis was performed on a Waters HPLC 600 Liquid Chromatograph (Milan, Italy) instrument equipped with a photo diode-array detector, DAD 2828 Waters. Data were processed with Empower™ 2 Waters Software. The analyses were running on a Gemini C18 (Phenomenex) column (250ˆ4.6 mm i.d.; 5 µm particle size). The mobile phase was water containing 0.1% (v/v) formic acid (A) and acetonitrile with 0.1% (v/v) of formic acid (B). The linear gradient started from 10% B and reached to 60% B in 60 min. The flow rate was 1 mL/min. UV spectra of the extract were conventionally recorded at 210, 270, 310 and 350 nm. All analyses were run in triplicate. An aliquot of 20 µL of each polar extract was injected for each run. For quantitative analysis, 5-level calibration curve was obtained by injection of known concentrations (10-250 µg/mL) of different standards compounds: luteolin-6-C-glucoside (y = 44625x + 119464; R 2 = 0.9992) and 5-O-caffeoylquinic acid (y = 32345x -58693; R 2 = 0.9999).  The 2,2-diphenyl-picryl hydrazyl (DPPH . ) radical-scavenging capacity was performed following Iriti et al. [51], with some modifications. In brief, aliquots of each sample, at five different concentrations (from 1-100 µM), were added to 0.07 mM MeOH solution of DPPH¨free radical reaching a final volume of 2 mL. After a reaction time of 30 min in the dark at room temperature, the decrease in absorbance was measured at 517 nm. The IC 50 was calculated with Prism ® 4 (GraphPad Software Inc., La Jolla, CA, USA).

ABTS¨+ Radical-Scavenging Assay
The 2,2 1 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS¨+) radical cation-scavenging capacity was determined according to Iriti et al. [51]. The ABTS¨+ radical cation was produced by reacting 7 mM ABTS with 2.45 mM potassium persulfate (final concentration) and maintaining the mixture in the dark at room temperature for at least 6 h before use. The ABTS¨+ solution was diluted with ethanol to an absorbance of 0.7 (˘0.02) at 734 nm and equilibrated at 30˝C. Ten µL of each sample, ethanol (negative control) and standard solution of the synthetic antioxidant 6-hydroxy-2,5,7,8-tetramethychroman-2-carboxylic acid (Trolox, positive control) were mixed for 30 s with one mL of diluted ABTS¨+ solution. Their absorbance was read at 734 nm, at room temperature, 50 s after the initial mixing. The results are expressed as Trolox equivalent antioxidant capacity (TEAC, µmol¨eq¨Trolox mL).

Antimicrobial Activity
The disk diffusion method was used according to the standard protocol of Bauer et al. [39] to assess antibacterial activities both of the investigated plant extracts and the essential oil. The following reference strains were included in the study: three Gram-positive (Bacillus cereus ATCC 999091, Enterococcus faecalis ATCC 29212, and Staphylococcus aureus ATCC 25923) and three Gram-negative (Escherichia coli ATCC 35218, Proteus mirabilis ATCC 12453, and Pseudomonas aeruginosa ATCC 27853). All organisms were maintained in brain-heart infusion (BHI medium Oxoid, Milan, Italy) containing 30% (v/v) glycerol at´80˝C. Before testing, the suspensions were transferred in BHI and overnight bacterial cultures were adjusted to 0.5 McFarland standards to achieve an inoculum of approximately 106 CFU/mL, and individually uniformly spread on Muller Hinton agar (MH, Oxoid, Milano) plates using a sterile swab. Each plate was dried for 15 min and then used for the sensitivity test. Sterile paper disks, 6 mm in diameter (Oxoid, Milano), which had previously been impregnated with 10 µL of each plant extract (MeOH, DCM, PET) and of the essential oil were dried, and then aseptically placed onto the surface of the inoculated media. Disks impregnated with extraction solvents were used as negative controls and disks with ceftazidime (30 µg) and erythromycin (5 µg) as references for Gram-negative and Gram-positive bacteria, respectively. After incubation at 37˝C for 18 h, plates were examined and the inhibition zones around the disks were measured (diameters in mm). Tests were repeated three times for each extract/microorganism combination.

Conclusions
This study reported, for the first time to the best of our knowledge, the characterization of the phenolic content and antibacterial activity of A. moschata, in addition to showing the high antiradical ability of the methanolic extract. Moreover, we have integrated the data available about the essential oil composition and we have presented the oil's early results related to its biological activity, which was never investigated before.
Our results, although preliminary, provide evidence to support some of the documented ethnobotanical uses of A. moschata that suggest its aerial parts can be considered a good source of bioactive compounds, potentially exploitable in the pharmaceutical field. To this end, future studies will focus on the identification of the antimicrobials in the most effective extracts, in addition to investigating other biological activities.