Chemistry and Pharmacology of Citrus sinensis

Presently the search for new drugs from natural resources is of growing interest to the pharmaceutical industry. Natural products have been the source of new drugs since ancient times. Plants are a good source of secondary metabolites which have been found to have beneficial properties. The present study is a review of the chemistry and pharmacology of Citrus sinensis. This review reveals the therapeutic potential of C. sinensis as a source of natural compounds with important activities that are beneficial for human health that could be used to develop new drugs.


Introduction
Natural products have been a rich source of compounds for drug discovery and offer larger scale structural diversity than synthetic compounds. Natural products have been major sources of bioactive agents and will continue to play a protagonist role in the discovery of new drugs [1]. The genus Citrus belongs to the family Rutaceae, This genus is the most important fruit tree crop in the world, with an annual production of approximately 123 million tons in 2010 [2,3]. Various species of Citrus are useful, such as C. limon (lemon), C. medica (citron), C. aurantium (sour orange), C. paradisi (grapefruit), C. reticulata (mandarin, tangerine), C. clementina (clementine) and C. sinensis (sweet orange) [4]. This review presents a botanical description of C. sinensis, its traditional uses, chemical composition, and pharmacological studies.                                            The results of these studies clearly demonstrate that orange is a good source of compounds that could have good potential for incorporation into human food products as functional ingredients and for new drugs, as we shall see from their pharmacological activities.

Antibacterial Activity
The antibacterial activity of essential oil, crude extracts and pure compounds of C. sinensis has been demonstrated in several studies. Silver nanoparticles synthesized at 25 °C and 60 °C using C. sinensis peel aqueous extract, showed diverse zones of inhibition using the agar well-diffusion method against Escherichia coli (25 °C 12.5 mm, 60 °C 16.0 mm), Pseudomonas aeruginosa (25 °C 11.7 mm, The results of these studies clearly demonstrate that orange is a good source of compounds that could have good potential for incorporation into human food products as functional ingredients and for new drugs, as we shall see from their pharmacological activities.

Antibacterial Activity
The antibacterial activity of essential oil, crude extracts and pure compounds of C. sinensis has been demonstrated in several studies. Silver nanoparticles synthesized at 25˝C and 60˝C using C. sinensis peel aqueous extract, showed diverse zones of inhibition using the agar well-diffusion method against Escherichia coli (25˝C 12.5 mm, 60˝C 16.0 mm), Pseudomonas aeruginosa (25˝C 11.7 mm, 60˝C 13.4 mm) and Staphylococcus aureus (25˝C 7.8 mm, 60˝C 9.2) [41]. Another study showed that silver nanoparticles synthesized by mixing silver nitrate solution with C. sinesis juice for 2 h at 37˝C displayed minimum inhibitory concentration (MIC) values of 20 µg/mL for Bacillus subtilis and Shigella and 30 µg/mL for S. aureus and E. coli. Antibiofilm activity of 80% to 90% was observed at 25 µg/mL [42]. Cold-pressed terpeneless (CPT) C. sinensis oil dissolved in ethanol or dimethylsulphoxide (DMSO) displayed MICs for Listeria monocytogenes at 0.3% and 0.25% v/v, and for Salmonella typhimurium at 1% v/v. Both ethanol and DMSO oil dispersion systems exhibited an intermediate MIC of 0.75% v/v for Lactobacillus plantarum [43]. CPT at 0.5% v/v also displayed an inhibition zone effect at 10 µL against S. aureus strains: methicillin-susceptible strain (31.50˘3.02 mm); methicillin-resistant strain (65.83˘3.76 mm); methicillin-and vancomycin intermediate-resistant strains (76.67˘4.08 mm and 32.50˘2.74, respectively). Inhibition of bacterial growth in the plates containing test oil was judged by comparing with the visible growth of untreated control plates [44]. The essential oil containing 1,8-cineole and hydrocarbons showed MIC 90% ě 10% (v/v) against P. aeruginosa [45]. Another study showed that C. sinensis oil showed diverse inhibition diameters with 0.1 mL of the oil against E. coli (18˘2 mm), L. monocytogenes (27˘2 mm), B. cereus (19˘2 mm) and S. aureus (14˘3 mm) [46]. Sweet orange oil and its major compounds decanal (73.  [47]. A mixture (1:1 v/v) of C. sinensis and C. bergamia essential oils showed inhibitory activity with a MIC value of 0.25%-0.5% (v/v) and a minimum inhibitory dose (MID) of 50 mg/L against vancomycin-susceptible and vancomycin-resistant Enterococcus faecium and E. faecalis, respectively. The predominant components of the mixture were limonene (45%-73%), citral (0.7%-3%) and linalool (0.5%-15%) [48,49]. Terpene oil obtained from C. sinensis essence produced 29.2˘3.7 mm zones of inhibition with 10 µL of oil, against eleven strains/serotypes of Salmonella (S. enteritidis, S. senftenberg, S. senftenberg, S. tennessee, S. kentucky, S. heidelberg, S. montevideo, S. michigan, S. typhimurium, and S. Stanley). The most predominant compound in the oil was d-limonene at a level of about 94%. Myrcene was the second most predominant compound, accounting for about 3% of the oil [50]. C. sinensis oil had strong antibacterial activities on L. monocytogenes, E. coli, S. enteritidis, P. mirabilis and B. cereus [51]. An anti-acne formulation based on C. sinensis (3%), Ocimum basilicum L (5%) essential oils and acetic acid (12%) inhibited Propionibacterium acnes. This antibacterial activity is due mainly to the 94.0% of limonene in C. sinensis, and limonene (2.54%), linalool (21.0%) and eugenol (7.17%) in O. basilicum L [52]. Peel hexane extract of C. sinensis displayed antimycobacterial activity against drug-sensitive (MIC 200 µL/mL), isoniazid-resistant (MIC 25 µL/mL), and ethambutol-resistant (MIC 50 µL/mL) variants of Mycobacterium tuberculosis H37Rv. Streptomycin showed a MIC value of 0.50 µL/mL for the sensitive strain whereas a MIC >8 µL/mL for the resistant strain. Isoniazid displayed a MIC value of 0.60 µL/mL for the sensitive strain whereas for the resistant strain was >1 µL/mL. Ethambutol gave a MIC value of 2 µL/mL for the sensitive strain and for the resistant strain was >32 µL/mL. Finally, rifampicin showed a MIC value of 0.60 µL/mL for the sensitive strain and a MIC >2 µL/mL for the resistant strain. In this study the standard drugs displayed better activity than the tested extracts. However, from the most active extract it is possible to obtain compounds with better activity than standard drugs [53]. Acetone and hexane extracts of C. sinensis leaf showed inhibition zones of 27 mm towards Helicobacter pylori. Clarithromycin (0.05 µg/mL) was added as positive control, and this antibiotic displayed better activity than the tested extracts. It is important to point out that active extracts could contain compounds with better activity [54]. The above results clearly indicate the wide antibacterial spectrum of C. sinensis thus justifying its use as antibacterial agent.

Antiparasitic Activity
Parasitic diseases, are serious worldwide public health problems, and C. sinensis is an alternative in the treatment and control of these diseases. The hexane (IC 50 42.13 µg/mL), chloroform (IC 50 88.03 µg/mL), ethyl acetate (IC 50 26.67 µg/mL), acetone (IC 50 > 100 µg/mL), and methanol (IC 50 > 100 µg/mL) extracts of C. sinensis peel, displayed moderate antimalarial activity against chloroquine (CQ)-sensitive (3D7) strain of Plasmodium falciparum. In this study, various standard drugs were used: artemisinin (3D7 strain IC 50 0.0045 µg/mL), chloroquine (3D7 strain IC 50 0.021 µg/mL), CQ diphosphate (D6 strain IC 50 0.00311 µg/mL), mefloquine (D6 strain IC 50 0.01608 µg/mL) and quinine (3D7 strain IC 50 0.02 µg/mL) [60]. The petroleum ether and methanol extracts of C. sinensis showed moderate antimalarial activity against P. falciparum FCK 2 strain having IC 50 values of 51.06 and 53.61 µg/mL, respectively. Untreated controls, consisting of parasitized red blood cells and 10 µL Ci of [ 35 S]-methionine were used [61]. Essential oil of C. sinensis peel showed that a dose of 0.4 g/mL caused death of Trypanosoma evansi in 3 min, and induced death of Trypanosoma brucei brucei in 5 min. A set of positive control (25 mg/mL of diminavetor), negative control (infected blood suspended in heparin and Phosphate Buffer Saline Glucose pH 7.2) and diluent control (pure vegetable oil 100%) were set up. Diminavetor induced total lysis after 2 min for both parasites [62]. Although extracts showed weak antiparasitic activity, there is the need for the separation, purification and structural elucidation of pure compounds from extracts and essential oil of C. sinensis in order to find potential antiparasitic drugs.

Antiproliferative Activity
A standardized extract of red orange juice obtained from three pigmented varieties of C. sinensis (Moro, Tarocco, Sanguinello) inhibited proliferation of normal human prostatic epithelial cell line PZ-HPV-7 at 10´3 g/mL and lung fibroblast cell line of Chinese hamsters V79-4 at 10´4 g/mL. Untreated controls just with 5ˆ104 cells/well were employed [63]. The juice of fruits of C. sinensis (L) Osb. (cv. Washington Navel and cv. Sanguinello) at concentrations of 82.6% and 73% showed 100% antiproliferative activity against the cell lines: K562 (human chronic myelogenous leukemia) and HL-60 (human leukemia). In the same way concentration of 10% showed 90.5% antiproliferative activity against MCF-7 cells (human breast adenocarcinoma) [64].

Antioxidant Activity
In recent years there has been increasing interest in plant antioxidants because of their potential health-promoting properties. The antioxidant activity of juices of C. sinensis acquired in a local supermarket based on kinetics of hydrogen peroxide scavenging displayed k values of 1.2˘0.3ˆ10 3 s´1 and 0.4˘0.1ˆ10 3 s´1. The significant variation in kinetic constants of H 2 O 2 elimination in food samples points to its potential use as a relative indicator and control of antioxidant activity [74]. The total antioxidant activity of Moro C. sinensis crude juice was evaluated on the basis of its ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH ‚ ), OH ‚ and 2,2 1 -azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS ‚`) radicals and to reduce iron. It was found that Moro juice efficiently scavenge ABTS radical cations reaching up to 64% of quenching corresponding to 14.30 µM using Trolox equivalents (TE) as the reference antioxidant, it also was able to scavenge DPPH radicals with an antioxidant power corresponding to 14.39˘0. 19 µM TE and eliminated about the 87% of hydroxyl radical generated at 16.40 µM TE. The above antioxidant activities are attributed to the presence of five C-glycosyl flavones: lucenin-2, vicenin-2, stellarin-2, lucenin-2-4 1 -methyl ether and scoparin; one 3-hydroxy-3-methylglutaryl glycosyl flavonol: 3-hydroxy-3-methylglutaryl glycosyl quercetin; and one flavone O-glycosides: chrysoeriol 7-O-neoesperidoside [75]. An amperometric biosensor for the quantification of the scavenging capacity of orange juices displayed values for natural orange juice at 1.01 mM of IC 50 50 30.3˘0.9 µM), caffeic acid (no significant results), gallic acid (no significant results), ferulic acid (no significant results), curcumin (no significant results), catechol (no significant results), quercetin (no significant results) were used for control and also for comparing. The orange juice showed the best activity [76]. Seed extract of C. sinensis exhibited antioxidant activity using reducing power and DPPH radical-scavenging assays, gallic acid (IC 50 = 29.5 µM) was used as control standard [77]. The flavonoid content of several methanolic extract fractions of Navel orange peel (flavedo and albedo of C. sinensis) was first analysed phytochemically and then assessed for its antioxidant activity in vitro. The chemical structures of the fractionated constituents were originally determined by comparing their retention times and the obtained UV spectral data with the available bibliographic data and further verified by detailed LC-DAD-MS (ESI+) analysis. The main flavonoid groups found within the fractions examined were polymethoxylated flavones, O-glycosylated flavones, C-glycosylated flavones, O-glycosylated flavonols, O-glycosylated flavanones and phenolic acids along with their ester derivatives. In addition, the quantitative HPLC analysis confirmed that hesperidin is the major flavonoid glycoside found in the orange peel. The antioxidant activity of the orange peel methanolic extract fractions was evaluated by DPPH assay and the Co(II)/EDTA-induced luminol chemiluminescence assay. Results showed that orange peel methanolic extracts possess moderate antioxidant activity as compared with the activity observed for the aglycones, diosmetin (EC 50 71.79˘13.58 mg and hesperetin (EC 50 29.18˘2.80 7 mg). Quercetin (EC 50 = 0.06 mg quercetin/mg DPPH) was used as positive control and the tested aglycones exhibited a significant higher hydroxyl scavenging activity than quercetin [78]. C. sinensis juice showed 84.81% DPPH antiradical effect at 100 µg/mL. In this study the ascorbic acid (96.36%) was used as positive control showing 96.36% DPPH antiradical effect [79]. The bound phenolic content of citrus peel showed DPPH scavenging at 1 mg/mL, OH scavenging at 4 mg/mL, Fe 2+ chelating ability at 0.48 mg/mL and the inhibition of Fe 2+ induced lipid peroxidation in pancreas at 142.8 µg/mL [80]. Acetone-water extract obtained from fresh edible part of red oranges fruits (C. sinensis, Torocco) displayed an intracellular antioxidant activity of 85% in Caco-2 cells at 50 mg/mL. Positive standard drugs-gallic acid and vitamin C-were used. The extract obtained from red oranges exhibited significant higher antioxidant activity than positive controls [81]. The antioxidant activity of methanol and ethanol extracts of C. sinensis peel showed a significant free radical scavenging activity generated by ABTS of 55.8% and 60.7%, respectively and DPPH scavenging activity based on its capability as hydrogen donator of 70% and 80%, respectively. Water (20-150 µL) and ascorbic acid were used as a control [82,83]. Dichloromethane extract and diethyl ether, ethyl acetate and n-butanol fractions obtained from methanolic extract showed scavenging activity expressed as EC 50 ranging from 3 to 1.1 mg dry extract/mg DPPH. The ethyl acetate fraction showed the best antioxidant activity, which is due to the presence of C-glycosylated flavones, O-glycosylated flavones, polymethoxylated flavones, O-glycosylated flavanones and esters of phenolic acids. The positive controls, ascorbic acid, trolox and quercetin were used. Trolox was found to be 7.2 times more active, ascorbic acid was 9.1 times more active and quercetin 10.9 times more active than ethyl acetate fraction [84,85].The current state of knowledge shows the benefits of C. sinensis as antioxidant therapeutic agent. Thus we encourage the use of C. sinensis as a nutraceutical agent.

Hypocholesterolemic Activity
Literature data shows that C. sinensis possesses beneficial properties related with cholesterol which is a serious health problem. The administration of lyophilized C. sinensis juice at a dose of 5 g/kg in aqueous vehicle in a volume of 0.5 mL/100 g body weight for 15 days on adult male Wistar rats (200-250 g), decreased plasma levels of cholesterol (31%), LDL (44%) and triglycerides (33%) [86]. Microsized insoluble fibers from C. sinensis fruits lowered the concentrations of serum triglycerides (15.6%-17.8%) and serum total cholesterol (15.7%-17.0%) by means of enhancing the excretion of cholesterol (123%-126%) and bile acids (129%-133%) in feces [87]. Studies of hypocholesterolemia activity of C. sinensis are few, however they are relevant and interesting, suggesting further research on this topic, which will be of great benefit, is warranted.

Anti-Obesity Activity
In the last years, several studies have recently evaluated the beneficial effects of C. sinensis and the active components in weight management and obesity. Moro juice extract (Morosil ® , 400 mg/die) was able to induce a significant reduction in body mass index (BMI) after 4 weeks of treatment. Moreover, in subjects treated with Moro extract, body weight, BMI, waist and hip circumference were significantly different from the placebo group. It was suggested that the active compounds anthocyanins, hydroxycinnamic acids, flavone glycosides and ascorbic acid contained in Moro juice have a synergistic effect on fat reduction in humans [88]. The effects of citrange (C. sinensisˆPoncirus trifoliata) fruit extracts in high-fat (HF) diet-induced obesity mice were studied. Female C57BL/6 mice were fed with a chow diet (control), an HF diet, HF diet supplemented with 1% w/w citrange peel extract (CPE) or 1% w/w citrange flesh and seed extract (CFSE) for 8 weeks. Results showed that both CPE and CFSE regulated the glucose metabolic disorders in obese mice. In CPE and CFSE-treated groups, the body weight, blood glucose, serum total cholesterol (TC) and low density lipoprotein cholesterol (LDL-c) levels were significantly reduced relative to those in the HF group. To explore the mechanisms of action of CPE and CFSE on the metabolism of glucose and lipid, related genes expressions in liver were assayed. In liver tissue, the expression level of peroxisome proliferator-activated receptor γ (PPARγ) and its target genes were down-regulated by CPE and CFSE as revealed by qPCR tests. In addition, both CPE and CFSE decreased the expression level of liver X receptor (LXR) α and β, both involved in lipid and glucose metabolism. Results suggested that CPE and CFSE administration could ameliorate obesity and metabolic disorders in HF diet-induced obesity mice probably through the inhibition of PPARγ and LXRs gene expressions. Among the main flavonoids of citrange extracts were naringin and poncirin both could be the bioactive compounds in this process [89]. Aqueous-methanol extracts of flavedo, albedo, and pulp of pooled samples of two varieties of Citrus fruits (C. reticulate and C. sinensis) efficiently prevented oxidative stress in human adipocytes with no cytotoxic effects [90]. Many medications have been used to manage obesity over the years. However, most of the anti-obesity drugs that were approved and marketed have now been withdrawn due to serious adverse effects, in this sense the results mentioned above reveal that the beneficial effects of citrus and the active components could be used in weight management and obesity.

Activity in Cardiovascular System
Varieties of Citrus are a rich source of dietary flavonoids which reduce the risk of adverse cardiovascular events. The drinking of commercial C. sinensis juice (CSJ) decreased diastolic and systolic blood pressure in 5.13% (p = 0.03) and´5.91% (p = 0.003) respectively in healthy volunteers using 500 mL/day of orange juice twice a day during four-weeks. However, the administration of natural CSJ during four weeks did not have significant effects on either diastolic or systolic blood pressure [91]. The water-ethanol and acetone leaf extracts of C. sinensis showed inotropic depression on the atria of guinea pigs of both sexes (300-500 g) having values of EC 50 of 300 µg/mL and 140 µg/mL, respectively. Drugs control naloxone (10 µM), propranolol (1.5 µM atropine sulfate), atropine sulfate (1 µM propranolol), did not change the effect of the crude extract [92]. In fact, cardiovascular medications are indicated for and/or used to treat many different disorders, conditions and diseases affecting the cardiovascular system, but in some cases could produce intensified effects, toxic levels, side effects and/or adverse effects. The results of these investigations show that oranges can be used therapeutically to treat this disease and could be safe.

Antiosteoporotic Activity
Problems related with osteoporosis, characterized by a loss of bone mass, is a major health problem that affect persons of advanced age. Citrus showed a potential protective activity against osteoporosis. Administration of ethanol extract of leaves and peel of C. sinensis (5 mg/kg) on ovariectomized rats, increased trabecular bone mineral content and bone mineral density of tibia as well as improved the levels of phosphorus and calcium reducing the bone loss [93]. The effects of feeding orange pulp on bone quality in a male rat with osteoporosis model improved some characteristics of bone structure [94]. Thus, alternative approaches and investigations for managing osteoporosis are needed and in this regard some evaluations of the anti-osteoporotic activity of C. sinensis, showed that it could be beneficial, safe and effective in management of osteoporosis.

Protective of UV Activity
Excessive UV radiation produces genetic mutations that could develop skin cancer. Standardized extract prepared from red orange (Bionap ® , 15 and 30 µg/mL) showed protective effects on ultraviolet B (shortwave) which induced damage in human keratinocytes. This activity could occur to the block of cellular oxidative stress-related events such as inflammation and apoptosis [95]. A supplement of red orange complex ® (C. sinensis varieties Moro, Tarocco and Sanguinello) produced by Bionap Company reduced UV-induced skin erythema on human healthy volunteers using a dose of 100 mg/daily for 15 days. Moreover, skin age spots pigmentation (melanin content) decreased from 27% to 7% when subjects were exposed to red orange extract [96]. The presented investigations demonstrate that C. sinensis protect cells from genotoxic effects of an UV radiation. Thus, orange can be a good candidate for sun protection products. However more research in this field is required.

Relaxant, Sedative and Anxiolytic Activities
Aromatherapy is the use of essential oils and extracts as an alternative treatment for medical purposes. Exposure to ambient odor of natural essential oil of C. sinensis showed a relaxant and sedative effect on dental patients [97]. The methanol and dichloromethane extracts obtained from the flowers of C. sinensis (L.) Osbeck showed a dose-dependent sedative effect in the exploratory cylinder model mice with an ED 50 (ip) values of 47.04˘12.03 mg/kg and 129.15˘21.25 mg/kg, respectively. Hesperidin (ED 50 = 11.34˘2.48 mg/kg) was identified in the methanol extract as the sedative active principle of this plant [98]. Anxiolytic activity of sweet orange aroma was demonstrated in forty male volunteers who were allocated to five different groups for the inhalation of sweet orange essential oil (test aroma: 2.5, 5, or 10 drops). Psychologic parameters (state-anxiety, subjective tension, tranquilization, and sedation) and physiologic parameters (heart rate and gastrocnemius electromyogram) were evaluated. Results gave scientific support to use as a tranquilizer by aromatherapists [99]. Sweet orange aroma (C. sinensis oil) in Wistar rats demonstrated anxiolytic-like activity. The animals were exposed to the orange aroma (100,200 or 400 µL) for 5 min within a plexiglass chamber and were then immediately submitted to the behavior tests. All doses of C. sinensis oil showed anxiolytic effect. Diazepam (2 mg/kg) was used as positive control. C. sinensis almost showed the same effect as diazepam [100]. Because of the well-recognized therapeutic potentials of extract and compounds of C. sinensis to present relaxant and sedative-hypnotic activities, orange represents an excellent natural alternative.
C. sinensis var. pear peel essential oil showed insecticidal activity against Bemisia tabaci at 8.5 µL/L which caused 97% mortality and LC 50 3.80 µL/L of air. Eugenol (LC 50 0.20 µ/L/L of air) was used as a positive control. Eugenol showed better fumigant action than essential oil [114]. C. sinensis essential oil showed strong toxic effect on eggs of Hyalomma dromedarii, especially in earlier embryonic development at concentration of 1:20 (oil: ethanol 95% v/v) [115]. The hexane extract of C. sinensis leaf possessed moderate larvicidal efficiency against dengue vector. The bioassays resulted in an LC 50 and LC 90 values of 446.84 and 1370.96 ppm, respectively after 24 h of exposure [116]. Coumarins xanthyletin and seselin, and the limonoid limonin isolated from hexane extract of C. sinensis leaves displayed in vitro activity on the growth of Xylella fastidiosa at concentrations ranging from 1.00 to 2.00 mg/mL. A blank experiment was performed with DMSO/H 2 O (1:1) alone (100 µL of bacterial culture, 33.5 µL of DMSO and 33.5 µL of H 2 O) in the well and was used as a positive control [117]. Extract of C. sinensis displayed strong bioactivity against Semia phis heraclei, Aphis craccivora, Tetranychus viennensis and T. trancatus [118]. The results of the experiments showed that natural products of C. sinensis clearly affect the growth of diverse plagues, so orange has the potential for use as alternative crop protectants against most likely pest species.
It is important to point out that literature includes three reviews of C. sinensis, one concerning the utilization of its nutrients to make multiple products of added value such as essential oil, pectin, yellow pigments, feed and enzymes [119]. The second review reported the chemical constituents of C. sinensis varieties [120], and the third is about the chemical composition of the essential oil of sweet orange [121]. Our review reported the chemical composition and pharmacological activities of C. sinensis varieties and the potential use of this plant as a source of bioactive compounds.

Conclusions
Natural products have been and will be important sources of new pharmaceutical compounds. Recently, there has been a renewed interest in natural product research due to the failure of alternative drug discovery methods to deliver many lead compounds in key therapeutic areas. In this sense, considering the health benefits of C. sinensis it presents excellent options for treating or helping in a disease due to its bioactive compounds (drug candidates) that show important activities or for developing new products, there is the need for public enlightenment on the importance of C. sinensis and finding and discovering new and effective drug compounds, so this review represents an excellent source of information about this natural product.