Design, Synthesis and Biological Evaluation of 6-(2,6-Dichloro-3,5-dimethoxyphenyl)-4-substituted-1H-indazoles as Potent Fibroblast Growth Factor Receptor Inhibitors

Tyrosine kinase fibroblast growth factor receptor (FGFR), which is aberrant in various cancer types, is a promising target for cancer therapy. Here we reported the design, synthesis, and biological evaluation of a new series of 6-(2,6-dichloro-3,5-dimethoxyphenyl)-4-substituted-1H-indazole derivatives as potent FGFR inhibitors. The compound 6-(2,6-dichloro-3,5-dimethoxyphenyl)-N-phenyl-1H-indazole-4-carboxamide (10a) was identified as a potent FGFR1 inhibitor, with good enzymatic inhibition. Further structure-based optimization revealed that 6-(2,6-dichloro-3,5-dimethoxyphenyl)-N-(3-(4-methylpiperazin-1-yl)phenyl)-1H-indazole-4-carboxamide (13a) is the most potent FGFR1 inhibitor in this series, with an enzyme inhibitory activity IC50 value of about 30.2 nM.

In recent years, several small molecule FGFR inhibitors have been reported, and some of them are now in clinical trials [15]. The early examples of FGFR inhibitors are predominantly multi-targeting drugs, such as nintedanib, lenvatinib, dovitinib, and lucitanib [16]. Although simultaneous inhibition of multiple RTKs may reinforce the efficacy in patients by concomitant disturbance of redundant pathways, it may also increase the chance of side effects or severe toxicity [17,18]. Thus, discovery of potent and selective FGFR inhibitors is an urgent need in cancer treatment. Currently, several discovery of potent and selective FGFR inhibitors is an urgent need in cancer treatment. Currently, several FGFR-selective inhibitors have progressed into clinical trials ( Figure 1) . JNJ-42756493 (1) is a potent oral pan-FGFR inhibitor with IC50 values in the low nanomolar range for FGFR1-4 and it was effective in vitro and in vivo in CRC tumors such as cell line NCI-H716 harboring amplified FGFR2 [19]. AZD4547 (2) is a highly potent and selective FGFR1-3 inhibitor. During the phase I trial, on-target activity was observed in five of 20 patients with tumors harboring FGFR signaling aberrations. Better efficacy was shown in patients with a high level of FGFR amplification [20]. NVP-BGJ398 (3), currently in phase II clinical trials, inhibits FGFR1-4 with low nanomolar potency at the molecular level (IC50 = 0.9, 1.4, 1.0, and 60 nM, respectively) and demonstrated at least 200-fold selectivity for FGFRs over other evaluated kinases [21]. Recently, several series of 6-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-indazole compounds 4 were reported as FGFR inhibitors [22,23]. Based on this promising scaffold and excellent bioactivity, we continued to investigate this chemotype in detail. Since the reported 6-(2,6-dichloro-3,5dimethoxyphenyl)-1H-indazole compounds all focused on the optimization at C3-position of indazole that extends into the solvent part in the FGFR binding site, we were wondering whether another position could be utilized to increase the binding activity. By taking advantage of docking studies, we hypothesized that the C4-position of indazole may be a new direction for optimization, as it can extend into a new binding subpocket in the ATP site of FGFR. Therefore, we designed and synthesized a new series of FGFR inhibitors containing 6-(2,6-dichloro-3,5-dimethoxyphenyl)-1Hindazole scaffold, and the FGFR1 enzymatic evaluation indeed showed the good bioactivity of this series of inhibitors.
Since the reported 6-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-indazole compounds all focused on the optimization at C3-position of indazole that extends into the solvent part in the FGFR binding site, we were wondering whether another position could be utilized to increase the binding activity. By taking advantage of docking studies, we hypothesized that the C4-position of indazole may be a new direction for optimization, as it can extend into a new binding subpocket in the ATP site of FGFR. Therefore, we designed and synthesized a new series of FGFR inhibitors containing 6-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-indazole scaffold, and the FGFR1 enzymatic evaluation indeed showed the good bioactivity of this series of inhibitors.

Inhibitor Design
To elucidate the interaction of the 6-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-indazole scaffold 4 with FGFR, we performed a docking study on compound 4 in the ATP site of FGFR1 using a reported crystal structure of the FGFR1 kinase domain (PDB ID: 3TT0), as shown in Figure 2A. According to this model, the NH at the N1-position of 1H-indazole is involved in a critical H-bond with the GLU562 carbonyl, and the N2 atom of the indazole ring forms another hydrogen bond with residue ALA564 located at the hinge region of the ATP-binding pocket. In addition, the two chlorine atoms, which formed favorable hydrophobic contacts with Val561 and Ala640, respectively, force the tetrasubstituted phenyl ring to adopt an almost perpendicular orientation with respect to the plane of the indazole ring. Overall the tetrasubstituted phenyl ring superimposes well with the NVP-BGJ398 in the 3TT0 co-crystal structure. Through this detailed analysis of the predicted bond conformation of compound 4, it was clear that the indazole C3 is an obvious direction for optimization as indicated by NVP-BGJ398. We also noticed that the methyl group at the amide moiety of NVP-BGJ398 is pointing to a subpocket, which could also be utilized for optimization. Therefore, we designed compound 10a and docked it into the FGFR1 binding site. As shown in Figure 2B, the 6-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-indazole scaffold still remains at the same position by forming the essential interactions described before, while the substituted benzyl amide group extends upward into a subpocket. Considering this interesting binding mode, we synthesized this compound and evaluated its activity in the FGFR1 enzymatic assay. The result confirmed that this compound 10a has good activity. Next, we selected compound 10a as the starting point for further modification. Compounds 13a-e were prepared according to the procedure shown in Scheme 2. Commercially available 15a-e were subjected to a reduction reaction with iron powder to afford 3-(4-substitutedpiperazin-1-yl)anilines 9a-e. Compounds 13a-e were prepared by condensation of compound 8 with compounds 9a-e. Compounds 13a-e were prepared according to the procedure shown in Scheme 2. Commercially available 15a-e were subjected to a reduction reaction with iron powder to afford 3-(4-substitutedpiperazin-1-yl)anilines 9a-e. Compounds 13a-e were prepared by condensation of compound 8 with compounds 9a-e.

Inhibitor Design
To elucidate the interaction of the 6-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-indazole scaffold 4 with FGFR, we performed a docking study on compound 4 in the ATP site of FGFR1 using a reported crystal structure of the FGFR1 kinase domain (PDB ID: 3TT0), as shown in Figure 2A. According to this model, the NH at the N1-position of 1H-indazole is involved in a critical H-bond with the GLU562 carbonyl, and the N2 atom of the indazole ring forms another hydrogen bond with residue ALA564 located at the hinge region of the ATP-binding pocket. In addition, the two chlorine atoms, which formed favorable hydrophobic contacts with Val561 and Ala640, respectively, force the tetrasubstituted phenyl ring to adopt an almost perpendicular orientation with respect to the plane of the indazole ring. Overall the tetrasubstituted phenyl ring superimposes well with the NVP-BGJ398 in the 3TT0 co-crystal structure. Through this detailed analysis of the predicted bond conformation of compound 4, it was clear that the indazole C3 is an obvious direction for optimization as indicated by NVP-BGJ398. We also noticed that the methyl group at the amide moiety of NVP-BGJ398 is pointing to a subpocket, which could also be utilized for optimization. Therefore, we designed compound 10a and docked it into the FGFR1 binding site. As shown in Figure 2B, the 6-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-indazole scaffold still remains at the same position by forming the essential interactions described before, while the substituted benzyl amide group extends upward into a subpocket. Considering this interesting binding mode, we synthesized this compound and evaluated its activity in the FGFR1 enzymatic assay. The result confirmed that this compound 10a has good activity. Next, we selected compound 10a as the starting point for further modification.

Inhibitor Design
To elucidate the interaction of the 6-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-indazole scaffold 4 with FGFR, we performed a docking study on compound 4 in the ATP site of FGFR1 using a reported crystal structure of the FGFR1 kinase domain (PDB ID: 3TT0), as shown in Figure 2A. According to this model, the NH at the N1-position of 1H-indazole is involved in a critical H-bond with the GLU562 carbonyl, and the N2 atom of the indazole ring forms another hydrogen bond with residue ALA564 located at the hinge region of the ATP-binding pocket. In addition, the two chlorine atoms, which formed favorable hydrophobic contacts with Val561 and Ala640, respectively, force the tetrasubstituted phenyl ring to adopt an almost perpendicular orientation with respect to the plane of the indazole ring. Overall the tetrasubstituted phenyl ring superimposes well with the NVP-BGJ398 in the 3TT0 co-crystal structure. Through this detailed analysis of the predicted bond conformation of compound 4, it was clear that the indazole C3 is an obvious direction for optimization as indicated by NVP-BGJ398. We also noticed that the methyl group at the amide moiety of NVP-BGJ398 is pointing to a subpocket, which could also be utilized for optimization. Therefore, we designed compound 10a and docked it into the FGFR1 binding site. As shown in Figure 2B, the 6-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-indazole scaffold still remains at the same position by forming the essential interactions described before, while the substituted benzyl amide group extends upward into a subpocket. Considering this interesting binding mode, we synthesized this compound and evaluated its activity in the FGFR1 enzymatic assay. The result confirmed that this compound 10a has good activity. Next, we selected compound 10a as the starting point for further modification.

The Structure-Activity Relationship of Indazole Derivatives
We first examined the impact of substitutions at the meta-or para-positions of the phenyl ring of compound 10a on the biological activity against FGFR1 (Table 1). Compared with compound 10a, introduction of an acetyl group at the meta-position of phenyl ring (compound 10b) caused a slight increase in inhibitory activity, while introducing an acetyl at the para-position (compound 10c) decreased the inhibitory activity. This trend is also reflected from the pair of compounds with methoxyl at the meta-position (10d) and para-position (10e). This might imply that substitution of the meta-position of phenyl ring could form favorable interactions with the binding site. The designed and synthesized compounds 11a-e with modifications on the phenyl moiety of compound 10d were assessed for their FGFR1 inhibitory activity ( Table 2). The results indicated that replacement of the phenyl ring (compound 10d) with pyridines (compounds 11a-c) or pyrimidines (compounds 11d,e) slightly decreased the activity. According to the obtained structure-activity relationship, we then prepared various compounds 12a-i with substitutions at the C3-position of the phenyl ring and evaluated them in the enzymatic assay ( Table 3). Incorporation of methoxycarbonyl (12a), ethoxycarbonyl (12b), nitro (12d), and 3-methyl-2-oxoimidazolidin-1-yl (12e) groups all reduced the enzymatic potency. Fortunately, compound containing a methylcarbamoyl at the phenyl 3-position (12b, IC50 = 38.6 ± 0.2 nM) showed better inhibitory activity than 10a (IC50 = 69.1 ± 19.8 nM).
Meanwhile, it was also found that incorporation of morpholine (12f) and piperazine (12g) could retain the enzymatic potency. However, compounds containing aromatic structures at the metaposition of phenyl displayed substantially reduced inhibitory activities.

The Structure-Activity Relationship of Indazole Derivatives
We first examined the impact of substitutions at the metaor para-positions of the phenyl ring of compound 10a on the biological activity against FGFR1 (Table 1). Compared with compound 10a, introduction of an acetyl group at the meta-position of phenyl ring (compound 10b) caused a slight increase in inhibitory activity, while introducing an acetyl at the para-position (compound 10c) decreased the inhibitory activity. This trend is also reflected from the pair of compounds with methoxyl at the meta-position (10d) and para-position (10e). This might imply that substitution of the meta-position of phenyl ring could form favorable interactions with the binding site.

The Structure-Activity Relationship of Indazole Derivatives
We first examined the impact of substitutions at the meta-or para-positions of the phenyl ring of compound 10a on the biological activity against FGFR1 (Table 1). Compared with compound 10a, introduction of an acetyl group at the meta-position of phenyl ring (compound 10b) caused a slight increase in inhibitory activity, while introducing an acetyl at the para-position (compound 10c) decreased the inhibitory activity. This trend is also reflected from the pair of compounds with methoxyl at the meta-position (10d) and para-position (10e). This might imply that substitution of the meta-position of phenyl ring could form favorable interactions with the binding site. The designed and synthesized compounds 11a-e with modifications on the phenyl moiety of compound 10d were assessed for their FGFR1 inhibitory activity ( Table 2). The results indicated that replacement of the phenyl ring (compound 10d) with pyridines (compounds 11a-c) or pyrimidines (compounds 11d,e) slightly decreased the activity. According to the obtained structure-activity relationship, we then prepared various compounds 12a-i with substitutions at the C3-position of the phenyl ring and evaluated them in the enzymatic assay ( Table 3). Incorporation of methoxycarbonyl (12a), ethoxycarbonyl (12b), nitro (12d), and 3-methyl-2-oxoimidazolidin-1-yl (12e) groups all reduced the enzymatic potency. Fortunately, compound containing a methylcarbamoyl at the phenyl 3-position (12b, IC50 = 38.6 ± 0.2 nM) showed better inhibitory activity than 10a (IC50 = 69.1 ± 19.8 nM).
Meanwhile, it was also found that incorporation of morpholine (12f) and piperazine (12g) could retain the enzymatic potency. However, compounds containing aromatic structures at the metaposition of phenyl displayed substantially reduced inhibitory activities. The IC 50 value of AZD4547 is 1.8 ± 0.1 nM (mean ± SD), which was significantly higher than that of any of the new compounds tested.
The designed and synthesized compounds 11a-e with modifications on the phenyl moiety of compound 10d were assessed for their FGFR1 inhibitory activity ( Table 2). The results indicated that replacement of the phenyl ring (compound 10d) with pyridines (compounds 11a-c) or pyrimidines (compounds 11d,e) slightly decreased the activity. According to the obtained structure-activity relationship, we then prepared various compounds 12a-i with substitutions at the C3-position of the phenyl ring and evaluated them in the enzymatic assay ( Table 3). Incorporation of methoxycarbonyl (12a), ethoxycarbonyl (12b), nitro (12d), and 3-methyl-2-oxoimidazolidin-1-yl (12e) groups all reduced the enzymatic potency. Fortunately, compound containing a methylcarbamoyl at the phenyl 3-position (12b, IC 50 = 38.6 ± 0.2 nM) showed better inhibitory activity than 10a (IC 50 = 69.1 ± 19.8 nM).
Meanwhile, it was also found that incorporation of morpholine (12f) and piperazine (12g) could retain the enzymatic potency. However, compounds containing aromatic structures at the meta-position of phenyl displayed substantially reduced inhibitory activities.                   In order to further improve the enzymatic potency of this series of compounds against FGFR1, various groups were incorporated at the 4'-position of the piperazine of compound 12g (Table 4). Compared to 12g, most of the resulting analogues 13b-e showed diminished enzymatic activities. Nevertheless, the 4-methylpiperazine analogue 13a demonstrated excellent inhibitory activity in the enzymatic assay (IC 50 = 30.2 ± 1.9 nM). In order to further improve the enzymatic potency of this series of compounds against FGFR1, various groups were incorporated at the 4'-position of the piperazine of compound 12g (Table 4). Compared to 12g, most of the resulting analogues 13b-e showed diminished enzymatic activities. Nevertheless, the 4-methylpiperazine analogue 13a demonstrated excellent inhibitory activity in the enzymatic assay (IC50 = 30.2 ± 1.9 nM).

General Information
1 H-NMR (400 MHz) and 13 C-NMR (125 MHz) spectra were recorded by using a Mercury-400 and a Mercury-500 High Performance Digital FT-NMR spectrometer, respectively (Varian, Palo Alto, CA, USA) with tetramethylsilane (TMS) as an internal standard. Abbreviations for peak patterns in NMR spectra are as follows: s = singlet, d = doublet, and m = multiplet. Low-resolution mass spectra were obtained with a LCQ Deca XP mass spectrometer (Finnigan, Palo Alto, CA, USA) using a CAPCELL PAK C18 (50 mm × 2.0 mm, 5 ZM) or an Agilent ZORBAX Eclipse XDB C18 (50 mm × 2.1 m, 5 ZM) in positive or negative electrospray mode. Low-resolution mass spectra and high-resolution mass spectra were recorded at an ionizing voltage of 70 eV on a Finnigan/MAT95 spectrometer. Compound purity was determined by analytical HPLC (Gilson, Middleton, WI, USA) using an YMC ODS3 column (50 mm × 4.6 mm, 5 ZM). Conditions were as follows: CH3CN/H2O eluent at 2.5 mL·min −1 flow [containing 0.1% trifluoroacetic acid (TFA)] at 35 °C, 8 min, gradient 5% CH3CN to 95% CH3CN, monitored by UV absorption at 214 nm and 254 nm. TLC analysis was carried out with glass precoated silica gel GF254 plates. TLC spots were visualized under UV light. Flash column chromatography was performed with a CombiFlash Rf system (Teledyne ISCO, Lincoln, NE, USA). All solvents and reagents were used directly as obtained commercially unless otherwise noted. Anhydrous dimethylformamide was purchased from Acros (Morris Plains, NJ, USA) and was used without further drying. All air and moisture sensitive reactions were carried out under an atmosphere of dry argon with heat-dried glassware and standard syringe techniques.  In order to further improve the enzymatic potency of this series of compounds against FGFR1, various groups were incorporated at the 4'-position of the piperazine of compound 12g (Table 4). Compared to 12g, most of the resulting analogues 13b-e showed diminished enzymatic activities. Nevertheless, the 4-methylpiperazine analogue 13a demonstrated excellent inhibitory activity in the enzymatic assay (IC50 = 30.2 ± 1.9 nM).

General Information
1 H-NMR (400 MHz) and 13 C-NMR (125 MHz) spectra were recorded by using a Mercury-400 and a Mercury-500 High Performance Digital FT-NMR spectrometer, respectively (Varian, Palo Alto, CA, USA) with tetramethylsilane (TMS) as an internal standard. Abbreviations for peak patterns in NMR spectra are as follows: s = singlet, d = doublet, and m = multiplet. Low-resolution mass spectra were obtained with a LCQ Deca XP mass spectrometer (Finnigan, Palo Alto, CA, USA) using a CAPCELL PAK C18 (50 mm × 2.0 mm, 5 ZM) or an Agilent ZORBAX Eclipse XDB C18 (50 mm × 2.1 m, 5 ZM) in positive or negative electrospray mode. Low-resolution mass spectra and high-resolution mass spectra were recorded at an ionizing voltage of 70 eV on a Finnigan/MAT95 spectrometer. Compound purity was determined by analytical HPLC (Gilson, Middleton, WI, USA) using an YMC ODS3 column (50 mm × 4.6 mm, 5 ZM). Conditions were as follows: CH3CN/H2O eluent at 2.5 mL·min −1 flow [containing 0.1% trifluoroacetic acid (TFA)] at 35 °C, 8 min, gradient 5% CH3CN to 95% CH3CN, monitored by UV absorption at 214 nm and 254 nm. TLC analysis was carried out with glass precoated silica gel GF254 plates. TLC spots were visualized under UV light. Flash column chromatography was performed with a CombiFlash Rf system (Teledyne ISCO, Lincoln, NE, USA). All solvents and reagents were used directly as obtained commercially unless otherwise noted. Anhydrous dimethylformamide was purchased from Acros (Morris Plains, NJ, USA) and was used without further drying. All air and moisture sensitive reactions were carried out under an atmosphere of dry argon with heat-dried glassware and standard syringe techniques. In order to further improve the enzymatic potency of this series of compounds against FGFR1, various groups were incorporated at the 4'-position of the piperazine of compound 12g (Table 4). Compared to 12g, most of the resulting analogues 13b-e showed diminished enzymatic activities. Nevertheless, the 4-methylpiperazine analogue 13a demonstrated excellent inhibitory activity in the enzymatic assay (IC50 = 30.2 ± 1.9 nM).

General Information
1 H-NMR (400 MHz) and 13 C-NMR (125 MHz) spectra were recorded by using a Mercury-400 and a Mercury-500 High Performance Digital FT-NMR spectrometer, respectively (Varian, Palo Alto, CA, USA) with tetramethylsilane (TMS) as an internal standard. Abbreviations for peak patterns in NMR spectra are as follows: s = singlet, d = doublet, and m = multiplet. Low-resolution mass spectra were obtained with a LCQ Deca XP mass spectrometer (Finnigan, Palo Alto, CA, USA) using a CAPCELL PAK C18 (50 mm × 2.0 mm, 5 ZM) or an Agilent ZORBAX Eclipse XDB C18 (50 mm × 2.1 m, 5 ZM) in positive or negative electrospray mode. Low-resolution mass spectra and high-resolution mass spectra were recorded at an ionizing voltage of 70 eV on a Finnigan/MAT95 spectrometer. Compound purity was determined by analytical HPLC (Gilson, Middleton, WI, USA) using an YMC ODS3 column (50 mm × 4.6 mm, 5 ZM). Conditions were as follows: CH3CN/H2O eluent at 2.5 mL·min −1 flow [containing 0.1% trifluoroacetic acid (TFA)] at 35 °C, 8 min, gradient 5% CH3CN to 95% CH3CN, monitored by UV absorption at 214 nm and 254 nm. TLC analysis was carried out with glass precoated silica gel GF254 plates. TLC spots were visualized under UV light. Flash column chromatography was performed with a CombiFlash Rf system (Teledyne ISCO, Lincoln, NE, USA). All solvents and reagents were used directly as obtained commercially unless otherwise noted. Anhydrous dimethylformamide was purchased from Acros (Morris Plains, NJ, USA) and was used without further drying. All air and moisture sensitive reactions were carried out under an atmosphere of dry argon with heat-dried glassware and standard syringe techniques. In order to further improve the enzymatic potency of this series of compounds against FGFR1, various groups were incorporated at the 4'-position of the piperazine of compound 12g (Table 4). Compared to 12g, most of the resulting analogues 13b-e showed diminished enzymatic activities. Nevertheless, the 4-methylpiperazine analogue 13a demonstrated excellent inhibitory activity in the enzymatic assay (IC50 = 30.2 ± 1.9 nM).

General Information
1 H-NMR (400 MHz) and 13 C-NMR (125 MHz) spectra were recorded by using a Mercury-400 and a Mercury-500 High Performance Digital FT-NMR spectrometer, respectively (Varian, Palo Alto, CA, USA) with tetramethylsilane (TMS) as an internal standard. Abbreviations for peak patterns in NMR spectra are as follows: s = singlet, d = doublet, and m = multiplet. Low-resolution mass spectra were obtained with a LCQ Deca XP mass spectrometer (Finnigan, Palo Alto, CA, USA) using a CAPCELL PAK C18 (50 mm × 2.0 mm, 5 ZM) or an Agilent ZORBAX Eclipse XDB C18 (50 mm × 2.1 m, 5 ZM) in positive or negative electrospray mode. Low-resolution mass spectra and high-resolution mass spectra were recorded at an ionizing voltage of 70 eV on a Finnigan/MAT95 spectrometer. Compound purity was determined by analytical HPLC (Gilson, Middleton, WI, USA) using an YMC ODS3 column (50 mm × 4.6 mm, 5 ZM). Conditions were as follows: CH3CN/H2O eluent at 2.5 mL·min −1 flow [containing 0.1% trifluoroacetic acid (TFA)] at 35 °C, 8 min, gradient 5% CH3CN to 95% CH3CN, monitored by UV absorption at 214 nm and 254 nm. TLC analysis was carried out with glass precoated silica gel GF254 plates. TLC spots were visualized under UV light. Flash column chromatography was performed with a CombiFlash Rf system (Teledyne ISCO, Lincoln, NE, USA). All solvents and reagents were used directly as obtained commercially unless otherwise noted. Anhydrous dimethylformamide was purchased from Acros (Morris Plains, NJ, USA) and was used without further drying. All air and moisture sensitive reactions were carried out under an atmosphere of dry argon with heat-dried glassware and standard syringe techniques.   To a solution of 2-(3,5-dimethoxyphenyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (6, 15 g, 56.82 mmol) in DMF (300 mL), NCS (16.7 g, 125.0 mmol) was added and the mixture was stirred at 90 • C for 2 h. The reaction was cooled to 25 • C and quenched with distilled water. The solid product was filtered off, washed with water, and dried (92%). 1 (8) To a solution of 6-(2,6-dichloro-3,5-dimethoxyphenyl)-N-phenyl-1H-indazole-4-carboxamide (7, 8.9 g, 23.35 mmol) in THF (120 mL) lithium hydroxide (3.92 g, 93.44 mmol) dissolved in distilled water (30 mL) was added. Then, the mixture was stirred at 50 • C for 6 h. The reaction was cooled to 25 • C. The reaction mixture was acidified with 1N HCl and the solid product was filtered off, washed with water, and dried (yield: 76.2%). 1  To a solution of 6-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-indazole-4-carboxylic acid (8, 30 mg, 0.082 mmol) in pyridine (3 mL) at 0 • C. POCl 3 (12 µL, 0.090 mmol) was added, and the mixture was stirred at 0 • C for 1 h. Aniline was added into the reaction mixture and stirred at 0 • C for 10 min, then, the mixture was stirred at 25 • C for 1.5 h. The reaction was quenched with distilled water, and the organic phase was washed with water, 1N HCl and brine, dried over Na 2 SO 4 , filtered and concentrated in vacuo. The resultant residue was purified by column chromatography to get the final product as a white solid.    To a solution of 6-(2,6-dichloro-3,5-dimethoxyphenyl)-1H-indazole-4-carboxylic acid (8) in dry DCM, DIPEA and HATU were added and the mixture was stirred at 25 • C for 4 h. The appropriate 3-(4-ethylpiperazin-1-yl)aniline 9a-e was then added and the mixture was stirred at 25 • C for 0.5 h. The reaction was quenched with distilled water, and the organic phase was washed with water and brine, dried over Na 2 SO 4 , filtered and concentrated in vacuo.

Conclusions
In summary, we have reported the design, synthesis, and biological evaluation of a novel series of 6-(2,6-dichloro-3,5-dimethoxyphenyl)-4-Substituted-1H-indazole derivatives as potent FGFR kinase inhibitors. Through four rounds of optimization, compound 13a stood out as the most potent FGFR1 inhibitor with an inhibition potency IC 50 = 30.2 ± 1.9 nM). We think current study not only suggests new potent FGFR inhibitors for the scientific community, but also points out a new direction for optimizing this promising scaffold.