Identification of Novel Human Breast Carcinoma (MDA-MB-231) Cell Growth Modulators from a Carbohydrate-Based Diversity Oriented Synthesis Library

The application of a cell-based growth inhibition on a library of skeletally different glycomimetics allowed for the selection of a hexahydro-2H-furo[3,2-b][1,4]oxazine compound as candidate inhibitors of MDA-MB-231 cell growth. Subsequent synthesis of analogue compounds and preliminary biological studies validated the selection of a valuable hit compound with a novel polyhydroxylated structure for the modulation of the breast carcinoma cell cycle mechanism.


Introduction
In the last 25 years, target-based drug discovery has become a paradigm in both the pharmaceutical industry and in academia.However, considering that it has proved hard to increase the number of truly innovative new drugs, the interest towards phenotypic screening of large small molecule libraries is growing fast [1,2].In this context, Diversity-Oriented Synthesis (DOS) [3][4][5] has proved to be very useful for the creation of high quality chemical libraries for early-stage drug discovery programmes.In fact, it operates to generate the maximum skeletal and stereochemical diversity by using forward synthetic analysis and complexity-generating reactions [6,7].Recently, natural product structures have proved to be optimal starting materials for DOS strategies [8][9][10], thanks to their structural feasibility to generate compounds in the medicinally relevant chemical space and following Lipinski's rules [11].In particular, carbohydrates can be exploited for the achievement of skeletal diversity, taking advantage of their conformational constraints and the high density of polar groups.Although the application of carbohydrates in traditional combinatorial chemistry has been reported since the nineties [12][13][14], they remain rather underexplored in DOS strategies, mainly because of their need for transitional protection/deprotection stages [15][16][17][18][19][20][21].In this context, we have reported the use of D-mannose in combination with glycine-derived aminoacetaldehyde for the synthesis of an array of novel skeletally different polyhydroxylated nitrogen-containing scaffolds [22].The relevance of these compounds was attested by the widespread distribution of polyhydroxylated nitrogen-containing natural products in plants and microorganisms (Figure 1).In particular iminosugars [23][24][25], pyrrolidine [26,27], and pyrrolizidine alkaloids [28][29][30][31][32] occupy relevant positions in biomedical issues, for their potential in the development of new treatments against cancer, infective diseases, diabetes, and metabolic disorders [33][34][35].DOS small molecule libraries are often applied in phenotypic screening or chemical genetics studies in search of hit compounds capable of inducing a desired phenotype, such as toxicity to bacteria or the ability to selectively kill cancer cells [36][37][38][39].This 'forward pharmacology' process is particularly successful for lead discovery in those complex disorders, such as cancer and neurological and infective pathologies, where multiple targets are involved and/or physiopathological pathways have yet to be discovered [40][41][42].Just to give some examples, nifedipine, nimodipine, and other calcium channel antagonists were actually discovered by the application of phenotypic screens in search for compounds able to induce vasodilatation and blood pressure reduction [43,44].In recent years, different phenotypic screening methods have been developed, especially in the pharmaceutical industries, were robotic and miniaturized technologies allow for rapidly screening large chemical collections.Different phenotypes can be taken into account, such as the capability of killing pathogens or cancer cells, or the modulation of autophagy and apoptosis, and cell cycle mechanisms are often the most studied ones [45].In particular, we reasoned to apply a cell-based phenotypic screening in search of compounds inducing a significant cell growth arrest on MDA-MB-231 cell lines.This cell line is a simple model system for the study of the triple-negative breast cancer (TNBC).This type of cancer shows a major tendency toward early metastasis [46,47], and does not respond to hormonal chemotherapy, as it lacks the three main molecular targets, the estrogen receptor (ER), the progesterone receptor (PR), and the human epidermal growth factor receptor (HER-2/Neu) [48][49][50].Therefore, the development of new treatments against such type of cancer, which accounts for 15% of all type of breast carcinomas, is highly needed.Although further research is still necessary, some preliminary evidence about the ability of iminosugars to inhibit breast cancer cell growth has recently appeared in the literature.In particular, different types of pyrrolidinic compounds have shown significant cell growth inhibition in breast tumoral cell lines, such as T-470 [51] and MCF-7 line [52].In addition, iminosugar-ferrocene conjugates proved to inhibit MDA-MB-231 breast cancer cells proliferation [53].
In this work we present the application of a MDA-MB-231 cell-based growth inhibition assay on a library of skeletally different glycomimetics, and the follow-up synthesis of compounds containing the same scaffold as of selected molecules for preliminary biological studies aiming to identify a valuable hit compound for the modulation of breast carcinoma cell cycle mechanism.

Results and Discussion
The diversity-oriented synthesis of six polyhydroxylated nitrogen-containing scaffolds was achieved by the combination of two building blocks, easily obtained from D-mannose, with glycinederived aminoacetaldehyde (Figure 2).Specifically, the synthesis of these compounds, as previously DOS small molecule libraries are often applied in phenotypic screening or chemical genetics studies in search of hit compounds capable of inducing a desired phenotype, such as toxicity to bacteria or the ability to selectively kill cancer cells [36][37][38][39].This 'forward pharmacology' process is particularly successful for lead discovery in those complex disorders, such as cancer and neurological and infective pathologies, where multiple targets are involved and/or physiopathological pathways have yet to be discovered [40][41][42].Just to give some examples, nifedipine, nimodipine, and other calcium channel antagonists were actually discovered by the application of phenotypic screens in search for compounds able to induce vasodilatation and blood pressure reduction [43,44].In recent years, different phenotypic screening methods have been developed, especially in the pharmaceutical industries, were robotic and miniaturized technologies allow for rapidly screening large chemical collections.Different phenotypes can be taken into account, such as the capability of killing pathogens or cancer cells, or the modulation of autophagy and apoptosis, and cell cycle mechanisms are often the most studied ones [45].In particular, we reasoned to apply a cell-based phenotypic screening in search of compounds inducing a significant cell growth arrest on MDA-MB-231 cell lines.This cell line is a simple model system for the study of the triple-negative breast cancer (TNBC).This type of cancer shows a major tendency toward early metastasis [46,47], and does not respond to hormonal chemotherapy, as it lacks the three main molecular targets, the estrogen receptor (ER), the progesterone receptor (PR), and the human epidermal growth factor receptor (HER-2/Neu) [48][49][50].Therefore, the development of new treatments against such type of cancer, which accounts for 15% of all type of breast carcinomas, is highly needed.Although further research is still necessary, some preliminary evidence about the ability of iminosugars to inhibit breast cancer cell growth has recently appeared in the literature.In particular, different types of pyrrolidinic compounds have shown significant cell growth inhibition in breast tumoral cell lines, such as T-470 [51] and MCF-7 line [52].In addition, iminosugar-ferrocene conjugates proved to inhibit MDA-MB-231 breast cancer cells proliferation [53].
In this work we present the application of a MDA-MB-231 cell-based growth inhibition assay on a library of skeletally different glycomimetics, and the follow-up synthesis of compounds containing the same scaffold as of selected molecules for preliminary biological studies aiming to identify a valuable hit compound for the modulation of breast carcinoma cell cycle mechanism.

Results and Discussion
The diversity-oriented synthesis of six polyhydroxylated nitrogen-containing scaffolds was achieved by the combination of two building blocks, easily obtained from D-mannose, with glycine-derived aminoacetaldehyde (Figure 2).Specifically, the synthesis of these compounds, as previously described [22], was achieved following the build/couple/pair approach by applying different synthetic strategies consisting of no more than four/five steps.
Molecules 2016, 21, 405 3 of 17 described [22], was achieved following the build/couple/pair approach by applying different synthetic strategies consisting of no more than four/five steps.A first screening on MDA-MB-231 human breast carcinoma cells was performed for the six compounds at 10 μM concentration.After the first 24 h of treatment, no particular effects on cell growth were observed (data not shown).Interestingly, after 48 h incubation, although a slightly induction in cell proliferation was detected for compounds, 2 3 and 6, we observed a significant reduction in cell proliferation following the treatment with compound 1, containing the hexahydro-2H-furo[3,2-b] [1,4]oxazine scaffold (p < 0.05), with a 40% inhibition of cell growth (Figure 3).A similar range of inhibition was found by others [54].Examination of cell morphology reveals essential information regarding the healthy status of a cell population.This inspection indicated that, after 48 h incubation, MDA-MB-231 cells were reduced in number, and part of the cell population showed a regular appearance, while part exhibited a round A first screening on MDA-MB-231 human breast carcinoma cells was performed for the six compounds at 10 µM concentration.After the first 24 h of treatment, no particular effects on cell growth were observed (data not shown).Interestingly, after 48 h incubation, although a slightly induction in cell proliferation was detected for compounds, 2, 3 and 6, we observed a significant reduction in cell proliferation following the treatment with compound 1, containing the hexahydro-2H-furo[3,2-b] [1,4]oxazine scaffold (p < 0.05), with a 40% inhibition of cell growth (Figure 3).A similar range of inhibition was found by others [54].
Molecules 2016, 21, 405 3 of 17 described [22], was achieved following the build/couple/pair approach by applying different synthetic strategies consisting of no more than four/five steps.A first screening on MDA-MB-231 human breast carcinoma cells was performed for the six compounds at 10 μM concentration.After the first 24 h of treatment, no particular effects on cell growth were observed (data not shown).Interestingly, after 48 h incubation, although a slightly induction in cell proliferation was detected for compounds, 2 3 and 6, we observed a significant reduction in cell proliferation following the treatment with compound 1, containing the hexahydro-2H-furo[3,2-b] [1,4]oxazine scaffold (p < 0.05), with a 40% inhibition of cell growth (Figure 3).A similar range of inhibition was found by others [54].Examination of cell morphology reveals essential information regarding the healthy status of a cell population.This inspection indicated that, after 48 h incubation, MDA-MB-231 cells were reduced in number, and part of the cell population showed a regular appearance, while part exhibited a round shape, though still adhering to the substrate.This observation suggests that the effect of compound Examination of cell morphology reveals essential information regarding the healthy status of a cell population.This inspection indicated that, after 48 h incubation, MDA-MB-231 cells were reduced in number, and part of the cell population showed a regular appearance, while part exhibited a round shape, though still adhering to the substrate.This observation suggests that the effect of compound 1 might induce a cell cycle slowdown and might not be related to any perturbation of the adhesive properties of the cell (Figure 4).In agreement with this, we found a dose-dependent cell growth inhibition, and we observed a significant reduction in cell proliferation at a concentration higher than 3 µM (Figure 5), with an IC 50 of 0.6 µM.In order to study and improve the activity of this compound, a pool of hexahydro-2H-furo[3,2-b] [1,4]oxazine compounds with different polyhydroxylated chains, stereochemistry, and amine functionalities, were synthetized following the two-step process as reported in Scheme 1 and Table 1.In order to study and improve the activity of this compound, a pool of hexahydro-2H-furo[3,2-b] [1,4]oxazine compounds with different polyhydroxylated chains, stereochemistry, and amine functionalities, were synthetized following the two-step process as reported in Scheme 1 and Table 1.In order to study and improve the activity of this compound, a pool of hexahydro-2H-furo[3,2-b] [1,4]oxazine compounds with different polyhydroxylated chains, stereochemistry, and amine functionalities, were synthetized following the two-step process as reported in Scheme 1 and Table 1.In order to study and improve the activity of this compound, a pool of hexahydro-2H-furo[3,2-b] [1,4]oxazine compounds with different polyhydroxylated chains, stereochemistry, and amine functionalities, were synthetized following the two-step process as reported in Scheme 1 and Table 1.The reaction of the sugar derivatives 7-10 with glycine-derived amino acetaldehyde by formation of the glycosyl amine and the direct N-acylation of the crude hemiaminal coupling intermediates resulted in the achievement of compounds 11-16.Then, under acid-catalyzed trans-acetalization conditions, the reaction of the C-3a hydroxyl group of the intermediate with the dimethylacetal carbon atom, led to the synthesis of the corresponding bicyclic compounds 17-20.In this way, as reported in Table 1, the compounds with different polyhydroxylated chains and different stereochemistry were obtained starting from the appropriate furanosidic monosaccharide.Specifically, from 2,3-O-isopropylidene-D-ribofuranose (7) and 2,3-O-isopropylidene-D-lyxofuranose (8) the corresponding Fmoc-protected intermediates 11α/11β and 12α/12β were obtained with a clean conversion and a successful separation of the two anomers, similarly to what obtained from 2,3:5,6-O-di-isopropylidene-D-mannose 10, as reported [22].The assignment of the configuration of the anomers was established by analysing the coupling constants between the protons of the CH2N-moiety of the corresponding deprotected mannosederived coupling intermediates, which are more stable and achievable by column chromatography, according to data reported in the literature for similar amino-α-D-mannofuranoses [55].
The preferred anomer of compounds 11-16 were found to be the α-anomer, even though starting from lyxose the ratio between the two anomeric compounds 12 became approximately 1:1 (Table 1, entry 2).The instability of the erythrose-derived coupling intermediate was evinced by the low yield of the reaction and in the achievement of only the most stable α-anomer product 13α (Table 1, entry 3).
Upon treating both the anomers of the coupling intermediates under neat TFA conditions, the corresponding bicyclic cis-fused scaffolds 17-20 were obtained, even if in less yield as compared to The reaction of the sugar derivatives 7-10 with glycine-derived amino acetaldehyde by formation of the glycosyl amine and the direct N-acylation of the crude hemiaminal coupling intermediates resulted in the achievement of compounds 11-16.Then, under acid-catalyzed trans-acetalization conditions, the reaction of the C-3a hydroxyl group of the intermediate with the dimethylacetal carbon atom, led to the synthesis of the corresponding bicyclic compounds 17-20.In this way, as reported in Table 1, the compounds with different polyhydroxylated chains and different stereochemistry were obtained starting from the appropriate furanosidic monosaccharide.Specifically, from 2,3-O-isopropylidene-D-ribofuranose (7) and 2,3-O-isopropylidene-D-lyxofuranose (8) the corresponding Fmoc-protected intermediates 11α/11β and 12α/12β were obtained with a clean conversion and a successful separation of the two anomers, similarly to what obtained from 2,3:5,6-O-di-isopropylidene-D-mannose 10, as reported [22].The assignment of the configuration of the anomers was established by analysing the coupling constants between the protons of the CH2N-moiety of the corresponding deprotected mannosederived coupling intermediates, which are more stable and achievable by column chromatography, according to data reported in the literature for similar amino-α-D-mannofuranoses [55].
The preferred anomer of compounds 11-16 were found to be the α-anomer, even though starting from lyxose the ratio between the two anomeric compounds 12 became approximately 1:1 (Table 1, entry 2).The instability of the erythrose-derived coupling intermediate was evinced by the low yield of the reaction and in the achievement of only the most stable α-anomer product 13α (Table 1, entry 3).
Upon treating both the anomers of the coupling intermediates under neat TFA conditions, the corresponding bicyclic cis-fused scaffolds 17-20 were obtained, even if in less yield as compared to The reaction of the sugar derivatives 7-10 with glycine-derived amino acetaldehyde by formation of the glycosyl amine and the direct N-acylation of the crude hemiaminal coupling intermediates resulted in the achievement of compounds 11-16.Then, under acid-catalyzed trans-acetalization conditions, the reaction of the C-3a hydroxyl group of the intermediate with the dimethylacetal carbon atom, led to the synthesis of the corresponding bicyclic compounds 17-20.In this way, as reported in Table 1, the compounds with different polyhydroxylated chains and different stereochemistry were obtained starting from the appropriate furanosidic monosaccharide.Specifically, from 2,3-O-isopropylidene-D-ribofuranose (7) and 2,3-O-isopropylidene-D-lyxofuranose (8) the corresponding Fmoc-protected intermediates 11α/11β and 12α/12β were obtained with a clean conversion and a successful separation of the two anomers, similarly to what obtained from 2,3:5,6-O-di-isopropylidene-D-mannose 10, as reported [22].The assignment of the configuration of the anomers was established by analysing the coupling constants between the protons of the CH2N-moiety of the corresponding deprotected mannosederived coupling intermediates, which are more stable and achievable by column chromatography, according to data reported in the literature for similar amino-α-D-mannofuranoses [55].
The preferred anomer of compounds 11-16 were found to be the α-anomer, even though starting from lyxose the ratio between the two anomeric compounds 12 became approximately 1:1 (Table 1, entry 2).The instability of the erythrose-derived coupling intermediate was evinced by the low yield of the reaction and in the achievement of only the most stable α-anomer product 13α (Table 1, entry 3).
Upon treating both the anomers of the coupling intermediates under neat TFA conditions, the corresponding bicyclic cis-fused scaffolds 17-20 were obtained, even if in less yield as compared to The reaction of the sugar derivatives 7-10 with glycine-derived amino acetaldehyde by formation of the glycosyl amine and the direct N-acylation of the crude hemiaminal coupling intermediates resulted in the achievement of compounds 11-16.Then, under acid-catalyzed trans-acetalization conditions, the reaction of the C-3a hydroxyl group of the intermediate with the dimethylacetal carbon atom, led to the synthesis of the corresponding bicyclic compounds 17-20.In this way, as reported in Table 1, the compounds with different polyhydroxylated chains and different stereochemistry were obtained starting from the appropriate furanosidic monosaccharide.Specifically, from 2,3-O-isopropylidene-D-ribofuranose (7) and 2,3-O-isopropylidene-D-lyxofuranose (8) the corresponding Fmoc-protected intermediates 11α/11β and 12α/12β were obtained with a clean conversion and a successful separation of the two anomers, similarly to what obtained from 2,3:5,6-O-di-isopropylidene-D-mannose 10, as reported [22].The assignment of the configuration of the anomers was established by analysing the coupling constants between the protons of the CH2N-moiety of the corresponding deprotected mannosederived coupling intermediates, which are more stable and achievable by column chromatography, according to data reported in the literature for similar amino-α-D-mannofuranoses [55].
The preferred anomer of compounds 11-16 were found to be the α-anomer, even though starting from lyxose the ratio between the two anomeric compounds 12 became approximately 1:1 (Table 1, entry 2).The instability of the erythrose-derived coupling intermediate was evinced by the low yield of the reaction and in the achievement of only the most stable α-anomer product 13α (Table 1, entry 3).
Upon treating both the anomers of the coupling intermediates under neat TFA conditions, the corresponding bicyclic cis-fused scaffolds 17-20 were obtained, even if in less yield as compared to The reaction of the sugar derivatives 7-10 with glycine-derived amino acetaldehyde by formation of the glycosyl amine and the direct N-acylation of the crude hemiaminal coupling intermediates resulted in the achievement of compounds 11-16.Then, under acid-catalyzed trans-acetalization conditions, the reaction of the C-3a hydroxyl group of the intermediate with the dimethylacetal carbon atom, led to the synthesis of the corresponding bicyclic compounds 17-20.In this way, as reported in Table 1, the compounds with different polyhydroxylated chains and different stereochemistry were obtained starting from the appropriate furanosidic monosaccharide.Specifically, from 2,3-O-isopropylidene-D-ribofuranose (7) and 2,3-O-isopropylidene-D-lyxofuranose (8) the corresponding Fmoc-protected intermediates 11α/11β and 12α/12β were obtained with a clean conversion and a successful separation of the two anomers, similarly to what obtained from 2,3:5,6-O-di-isopropylidene-D-mannose 10, as reported [22].The assignment of the configuration of the anomers was established by analysing the coupling constants between the protons of the CH2N-moiety of the corresponding deprotected mannosederived coupling intermediates, which are more stable and achievable by column chromatography, according to data reported in the literature for similar amino-α-D-mannofuranoses [55].
The preferred anomer of compounds 11-16 were found to be the α-anomer, even though starting from lyxose the ratio between the two anomeric compounds 12 became approximately 1:1 (Table 1, entry 2).The instability of the erythrose-derived coupling intermediate was evinced by the low yield of the reaction and in the achievement of only the most stable α-anomer product 13α (Table 1, entry 3).
Upon treating both the anomers of the coupling intermediates under neat TFA conditions, the The reaction of the sugar derivatives 7-10 with glycine-derived amino acetaldehyde by formation of the glycosyl amine and the direct N-acylation of the crude hemiaminal coupling intermediates resulted in the achievement of compounds 11-16.Then, under acid-catalyzed trans-acetalization conditions, the reaction of the C-3a hydroxyl group of the intermediate with the dimethylacetal carbon atom, led to the synthesis of the corresponding bicyclic compounds 17-20.In this way, as reported in Table 1, the compounds with different polyhydroxylated chains and different stereochemistry were obtained starting from the appropriate furanosidic monosaccharide.Specifically, from 2,3-O-isopropylidene-D-ribofuranose (7) and 2,3-O-isopropylidene-D-lyxofuranose (8) the corresponding Fmoc-protected intermediates 11α/11β and 12α/12β were obtained with a clean conversion and a successful separation of the two anomers, similarly to what obtained from 2,3:5,6-O-di-isopropylidene-D-mannose 10, as reported [22].The assignment of the configuration of the anomers was established by analysing the coupling constants between the protons of the CH2N-moiety of the corresponding deprotected mannosederived coupling intermediates, which are more stable and achievable by column chromatography, according to data reported in the literature for similar amino-α-D-mannofuranoses [55].
The preferred anomer of compounds 11-16 were found to be the α-anomer, even though starting from lyxose the ratio between the two anomeric compounds 12 became approximately 1:1 (Table 1, entry 2).The instability of the erythrose-derived coupling intermediate was evinced by the low yield of the reaction and in the achievement of only the most stable α-anomer product 13α (Table 1, entry 3).
Upon treating both the anomers of the coupling intermediates under neat TFA conditions, the The reaction of the sugar derivatives 7-10 with glycine-derived amino acetaldehyde by formation of the glycosyl amine and the direct N-acylation of the crude hemiaminal coupling intermediates resulted in the achievement of compounds 11-16.Then, under acid-catalyzed trans-acetalization conditions, the reaction of the C-3a hydroxyl group of the intermediate with the dimethylacetal carbon atom, led to the synthesis of the corresponding bicyclic compounds 17-20.In this way, as reported in Table 1, the compounds with different polyhydroxylated chains and different stereochemistry were obtained starting from the appropriate furanosidic monosaccharide.Specifically, from 2,3-O-isopropylidene-D-ribofuranose (7) and 2,3-O-isopropylidene-D-lyxofuranose (8) the corresponding Fmoc-protected intermediates 11α/11β and 12α/12β were obtained with a clean conversion and a successful separation of the two anomers, similarly to what obtained from 2,3:5,6-O-di-isopropylidene-D-mannose 10, as reported [22].The assignment of the configuration of the anomers was established by analysing the coupling constants between the protons of the CH2N-moiety of the corresponding deprotected mannosederived coupling intermediates, which are more stable and achievable by column chromatography, according to data reported in the literature for similar amino-α-D-mannofuranoses [55].
The preferred anomer of compounds 11-16 were found to be the α-anomer, even though starting from lyxose the ratio between the two anomeric compounds 12 became approximately 1:1 (Table 1, entry 2).The instability of the erythrose-derived coupling intermediate was evinced by the low yield of the reaction and in the achievement of only the most stable α-anomer product 13α (Table 1, entry 3).
Upon treating both the anomers of the coupling intermediates under neat TFA conditions, the The reaction of the sugar derivatives 7-10 with glycine-derived amino acetaldehyde by formation of the glycosyl amine and the direct N-acylation of the crude hemiaminal coupling intermediates resulted in the achievement of compounds 11-16.Then, under acid-catalyzed trans-acetalization conditions, the reaction of the C-3a hydroxyl group of the intermediate with the dimethylacetal carbon atom, led to the synthesis of the corresponding bicyclic compounds 17-20.In this way, as reported in Table 1, the compounds with different polyhydroxylated chains and different stereochemistry were obtained starting from the appropriate furanosidic monosaccharide.Specifically, from 2,3-O-isopropylidene-D-ribofuranose (7) and 2,3-O-isopropylidene-D-lyxofuranose (8) the corresponding Fmoc-protected intermediates 11α/11β and 12α/12β were obtained with a clean conversion and a successful separation of the two anomers, similarly to what obtained from 2,3:5,6-O-di-isopropylidene-D-mannose 10, as reported [22].The assignment of the configuration of the anomers was established by analysing the coupling constants between the protons of the CH2N-moiety of the corresponding deprotected mannosederived coupling intermediates, which are more stable and achievable by column chromatography, according to data reported in the literature for similar amino-α-D-mannofuranoses [55].
The preferred anomer of compounds 11-16 were found to be the α-anomer, even though starting from lyxose the ratio between the two anomeric compounds 12 became approximately 1:1 (Table 1, entry 2).The instability of the erythrose-derived coupling intermediate was evinced by the low yield of the reaction and in the achievement of only the most stable α-anomer product 13α (Table 1, entry 3).
Upon treating both the anomers of the coupling intermediates under neat TFA conditions, the The reaction of the sugar derivatives 7-10 with glycine-derived amino acetaldehyde by formation of the glycosyl amine and the direct N-acylation of the crude hemiaminal coupling intermediates resulted in the achievement of compounds 11-16.Then, under acid-catalyzed trans-acetalization conditions, the reaction of the C-3a hydroxyl group of the intermediate with the dimethylacetal carbon atom, led to the synthesis of the corresponding bicyclic compounds 17-20.In this way, as reported in Table 1, the compounds with different polyhydroxylated chains and different stereochemistry were obtained starting from the appropriate furanosidic monosaccharide.Specifically, from 2,3-O-isopropylidene-D-ribofuranose (7) and 2,3-O-isopropylidene-D-lyxofuranose (8) the corresponding Fmoc-protected intermediates 11α/11β and 12α/12β were obtained with a clean conversion and a successful separation of the two anomers, similarly to what obtained from 2,3:5,6-O-di-isopropylidene-D-mannose 10, as reported [22].The assignment of the configuration of the anomers was established by analysing the coupling constants between the protons of the CH2N-moiety of the corresponding deprotected mannosederived coupling intermediates, which are more stable and achievable by column chromatography, according to data reported in the literature for similar amino-α-D-mannofuranoses [55].
The preferred anomer of compounds 11-16 were found to be the α-anomer, even though starting from lyxose the ratio between the two anomeric compounds 12 became approximately 1:1 (Table 1, entry 2).The instability of the erythrose-derived coupling intermediate was evinced by the low yield of the reaction and in the achievement of only the most stable α-anomer product 13α (Table 1, entry 3).
Upon treating both the anomers of the coupling intermediates under neat TFA conditions, the The reaction of the sugar derivatives 7-10 with glycine-derived amino acetaldehyde by formation of the glycosyl amine and the direct N-acylation of the crude hemiaminal coupling intermediates resulted in the achievement of compounds 11-16.Then, under acid-catalyzed trans-acetalization conditions, the reaction of the C-3a hydroxyl group of the intermediate with the dimethylacetal carbon atom, led to the synthesis of the corresponding bicyclic compounds 17-20.In this way, as reported in Table 1, the compounds with different polyhydroxylated chains and different stereochemistry were obtained starting from the appropriate furanosidic monosaccharide.Specifically, from 2,3-O-isopropylidene-D-ribofuranose (7) and 2,3-O-isopropylidene-D-lyxofuranose (8) the corresponding Fmoc-protected intermediates 11α/11β and 12α/12β were obtained with a clean conversion and a successful separation of the two anomers, similarly to what obtained from 2,3:5,6-O-di-isopropylidene-D-mannose 10, as reported [22].The assignment of the configuration of the anomers was established by analysing the coupling constants between the protons of the CH2N-moiety of the corresponding deprotected mannosederived coupling intermediates, which are more stable and achievable by column chromatography, according to data reported in the literature for similar amino-α-D-mannofuranoses [55].
The preferred anomer of compounds 11-16 were found to be the α-anomer, even though starting from lyxose the ratio between the two anomeric compounds 12 became approximately 1:1 (Table 1, entry 2).The instability of the erythrose-derived coupling intermediate was evinced by the low yield of the reaction and in the achievement of only the most stable α-anomer product 13α (Table 1, entry 3).
Upon treating both the anomers of the coupling intermediates under neat TFA conditions, the The reaction of the sugar derivatives 7-10 with glycine-derived amino acetaldehyde by formation of the glycosyl amine and the direct N-acylation of the crude hemiaminal coupling intermediates resulted in the achievement of compounds 11-16.Then, under acid-catalyzed trans-acetalization conditions, the reaction of the C-3a hydroxyl group of the intermediate with the dimethylacetal carbon atom, led to the synthesis of the corresponding bicyclic compounds 17-20.In this way, as reported in Table 1, the compounds with different polyhydroxylated chains and different stereochemistry were obtained starting from the appropriate furanosidic monosaccharide.Specifically, from 2,3-O-isopropylidene-D-ribofuranose (7) and 2,3-O-isopropylidene-D-lyxofuranose (8) the corresponding Fmoc-protected intermediates 11α/11β and 12α/12β were obtained with a clean conversion and a successful separation of the two anomers, similarly to what obtained from 2,3:5,6-O-di-isopropylidene-D-mannose 10, as reported [22].The assignment of the configuration of the anomers was established by analysing the coupling constants between the protons of the CH2N-moiety of the corresponding deprotected mannosederived coupling intermediates, which are more stable and achievable by column chromatography, according to data reported in the literature for similar amino-α-D-mannofuranoses [55].
The preferred anomer of compounds 11-16 were found to be the α-anomer, even though starting from lyxose the ratio between the two anomeric compounds 12 became approximately 1:1 (Table 1, entry 2).The instability of the erythrose-derived coupling intermediate was evinced by the low yield of the reaction and in the achievement of only the most stable α-anomer product 13α (Table 1, entry 3).
Upon treating both the anomers of the coupling intermediates under neat TFA conditions, the 20, 65% The reaction of the sugar derivatives 7-10 with glycine-derived amino acetaldehyde by formation of the glycosyl amine and the direct N-acylation of the crude hemiaminal coupling intermediates resulted in the achievement of compounds 11-16.Then, under acid-catalyzed trans-acetalization conditions, the reaction of the C-3a hydroxyl group of the intermediate with the dimethylacetal carbon atom, led to the synthesis of the corresponding bicyclic compounds 17-20.In this way, as reported in Table 1, the compounds with different polyhydroxylated chains and different stereochemistry were obtained starting from the appropriate furanosidic monosaccharide.Specifically, from 2,3-O-isopropylidene-D-ribofuranose (7) and 2,3-O-isopropylidene-D-lyxofuranose (8) the corresponding Fmoc-protected intermediates 11α/11β and 12α/12β were obtained with a clean conversion and a successful separation of the two anomers, similarly to what obtained from 2,3:5,6-O-di-isopropylidene-D-mannose 10, as reported [22].The assignment of the configuration of the anomers was established by analysing the coupling constants between the protons of the CH 2 N-moiety of the corresponding deprotected mannose-derived coupling intermediates, which are more stable and achievable by column chromatography, according to data reported in the literature for similar amino-α-D-mannofuranoses [55].
The preferred anomer of compounds 11-16 were found to be the α-anomer, even though starting from lyxose the ratio between the two anomeric compounds 12 became approximately 1:1 (Table 1, entry 2).The instability of the erythrose-derived coupling intermediate was evinced by the low yield of the reaction and in the achievement of only the most stable α-anomer product 13α (Table 1, entry 3).
Upon treating both the anomers of the coupling intermediates under neat TFA conditions, the corresponding bicyclic cis-fused scaffolds 17-20 were obtained, even if in less yield as compared to the mannose-derived bicycle 1 (entry 4).In all cases, the same scaffold was achieved from both the anomers in similar yields as a consequence of a thermodynamic equilibration of N-Fmoc hemiaminal intermediate species under the acidic treatment [56].
The cis-fusion was evinced by NOESY1D experiments of the fully acetylated compound 21 (Scheme 2 and Figure 6).The same cis-fusion was evinced for the other scaffolds thanks to the diagnostic signal of the bridgehead protons which appeared as singlets in a diagnostic and unambiguous region of 1 H-NMR spectrum between 5.70 and 4.70 ppm.In all cases only the endo anomer was recovered, which is the most stable for stereoelectronic effects.The derivatization of the unstable hemiaminal coupling intermediate was possible only using benzoyl chloride (entry 5) and chloroformates (entries 4 and 6).However, even if the use of chloroformate resulted in a clean cyclization to the final product, benzoyl analogues (15α/15β) failed to give the corresponding bicyclic compound, as a consequence of the reduced stability of the N-acyl moiety.
The reaction of the hemiaminal intermediate with sulfonyl chloride was not possible at all.However, in order to explore the possibility of introducing a different moiety linked to the amine and studying their biological effect, the tosyl derivative 23 was obtained from the corresponding per-Oacetylated N-deprotected 22 (Scheme 2) subsequent to Fmoc deprotection of 21.
In order to assess the stability of this class of bicyclic compounds based on the hexahydro-2Hfuro[3,2-b] [1,4]oxazine scaffold concerning the hemiaminal moiety under acidic conditions, a study by HPLC analysis was performed on 18 as a representative compound in MeOH and acetonitrile as solvents and in the presence of 1 M HCl, after 1 h and 24 h.In all cases, neither the formation of byproducts nor the reduction of peak intensity was evinced, as compared to benzophenone as an internal standard (see Supplementary Materials for HPLC data).
These new compounds were tested for their inhibition activity at 10 μM concentration after 48 h of incubation on the same MDA-MB-231 cell line.As reported in Table 2, none of these compounds proved to be as interesting as 1, revealing a specific structural requirement for an optimal inhibition  The derivatization of the unstable hemiaminal coupling intermediate was possible only using benzoyl chloride (entry 5) and chloroformates (entries 4 and 6).However, even if the use of chloroformate resulted in a clean cyclization to the final product, benzoyl analogues (15α/15β) failed to give the corresponding bicyclic compound, as a consequence of the reduced stability of the N-acyl moiety.
The reaction of the hemiaminal intermediate with sulfonyl chloride was not possible at all.However, in order to explore the possibility of introducing a different moiety linked to the amine and studying their biological effect, the tosyl derivative 23 was obtained from the corresponding per-Oacetylated N-deprotected 22 (Scheme 2) subsequent to Fmoc deprotection of 21.
In order to assess the stability of this class of bicyclic compounds based on the hexahydro-2Hfuro[3,2-b] [1,4]oxazine scaffold concerning the hemiaminal moiety under acidic conditions, a study by HPLC analysis was performed on 18 as a representative compound in MeOH and acetonitrile as solvents and in the presence of 1 M HCl, after 1 h and 24 h.In all cases, neither the formation of byproducts nor the reduction of peak intensity was evinced, as compared to benzophenone as an internal standard (see Supplementary Materials for HPLC data).
These new compounds were tested for their inhibition activity at 10 μM concentration after 48 h of incubation on the same MDA-MB-231 cell line.As reported in Table 2, none of these compounds proved to be as interesting as 1, revealing a specific structural requirement for an optimal inhibition The derivatization of the unstable hemiaminal coupling intermediate was possible only using benzoyl chloride (entry 5) and chloroformates (entries 4 and 6).However, even if the use of chloroformate resulted in a clean cyclization to the final product, benzoyl analogues (15α/15β) failed to give the corresponding bicyclic compound, as a consequence of the reduced stability of the N-acyl moiety.
The reaction of the hemiaminal intermediate with sulfonyl chloride was not possible at all.However, in order to explore the possibility of introducing a different moiety linked to the amine and studying their biological effect, the tosyl derivative 23 was obtained from the corresponding per-O-acetylated N-deprotected 22 (Scheme 2) subsequent to Fmoc deprotection of 21.
In order to assess the stability of this class of bicyclic compounds based on the hexahydro-2Hfuro[3,2-b] [1,4]oxazine scaffold concerning the hemiaminal moiety under acidic conditions, a study by HPLC analysis was performed on 18 as a representative compound in MeOH and acetonitrile as solvents and in the presence of 1 M HCl, after 1 h and 24 h.In all cases, neither the formation of by-products nor the reduction of peak intensity was evinced, as compared to benzophenone as an internal standard (see Supplementary Materials for HPLC data).These new compounds were tested for their inhibition activity at 10 µM concentration after 48 h of incubation on the same MDA-MB-231 cell line.As reported in Table 2, none of these compounds proved to be as interesting as 1, revealing a specific structural requirement for an optimal inhibition profile.The importance of the number hydroxyl groups and their stereochemistry for the activity was evinced by comparing the inhibition activities of Fmoc-derivatives 1 and 17-19, and those of 1 and the corresponding fully acetylated derivative 21.Finally, an indication of the importance of the functionalization of the hemiaminal nitrogen atom was shown by comparing the inhibition activities of 1, 20, and 23, all possessing similar scaffold and polyhydroxylated tail, and differing by the Fmoc, Cbz and tosyl group at the nitrogen atom, respectively.All in all, the biological data suggested a specific effect of the bicyclic compounds towards cell growth inhibition of MDA-MB-231 cells, as any small change in the structure or appendage of the lead compound 1 caused a drop in the biological activity.effect on cell adhesion was found, however, after the 48 h treatment, the very few cells present in the supernatant and the adherent cells were grouped together and no differences were observed between the diverse cell treatments in terms of apoptosis (Figure 8, lower panel).Overall, it is possible to assume that the growth inhibition induced by compound 1 on MDA-MB-231 cells, might be correlated to a cytostatic effect, rather than to a cytotoxic one.Thus, the exploration of the fine mechanisms that regulate the cell cycle will be performed in future to identify the protein target of compound 1, and future experiments on non-transformed epithelial cells will be performed to ensure the selectivity and specificity of the compounds [57].effect on cell adhesion was found, however, after the 48 h treatment, the very few cells present in the supernatant and the adherent cells were grouped together and no differences were observed between the diverse cell treatments in terms of apoptosis (Figure 8, lower panel).Overall, it is possible to assume that the growth inhibition induced by compound 1 on MDA-MB-231 cells, might be correlated to a cytostatic effect, rather than to a cytotoxic one.Thus, the exploration of the fine mechanisms that regulate the cell cycle will be performed in future to identify the protein target of compound 1, and future experiments on non-transformed epithelial cells will be performed to ensure the selectivity and specificity of the compounds [57].effect on cell adhesion was found, however, after the 48 h treatment, the very few cells present in the supernatant and the adherent cells were grouped together and no differences were observed between the diverse cell treatments in terms of apoptosis (Figure 8, lower panel).Overall, it is possible to assume that the growth inhibition induced by compound 1 on MDA-MB-231 cells, might be correlated to a cytostatic effect, rather than to a cytotoxic one.Thus, the exploration of the fine mechanisms that regulate the cell cycle will be performed in future to identify the protein target of compound 1, and future experiments on non-transformed epithelial cells will be performed to ensure the selectivity and specificity of the compounds [57].effect on cell adhesion was found, however, after the 48 h treatment, the very few cells present in the supernatant and the adherent cells were grouped together and no differences were observed between the diverse cell treatments in terms of apoptosis (Figure 8, lower panel).Overall, it is possible to assume that the growth inhibition induced by compound 1 on MDA-MB-231 cells, might be correlated to a cytostatic effect, rather than to a cytotoxic one.Thus, the exploration of the fine mechanisms that regulate the cell cycle will be performed in future to identify the protein target of compound 1, and future experiments on non-transformed epithelial cells will be performed to ensure the selectivity and specificity of the compounds [57].effect on cell adhesion was found, however, after the 48 h treatment, the very few cells present in the supernatant and the adherent cells were grouped together and no differences were observed between the diverse cell treatments in terms of apoptosis (Figure 8, lower panel).Overall, it is possible to assume that the growth inhibition induced by compound 1 on MDA-MB-231 cells, might be correlated to a cytostatic effect, rather than to a cytotoxic one.Thus, the exploration of the fine mechanisms that regulate the cell cycle will be performed in future to identify the protein target of compound 1, and future experiments on non-transformed epithelial cells will be performed to ensure the selectivity and specificity of the compounds [57].effect on cell adhesion was found, however, after the 48 h treatment, the very few cells present in the supernatant and the adherent cells were grouped together and no differences were observed between the diverse cell treatments in terms of apoptosis (Figure 8, lower panel).Overall, it is possible to assume that the growth inhibition induced by compound 1 on MDA-MB-231 cells, might be correlated to a cytostatic effect, rather than to a cytotoxic one.Thus, the exploration of the fine mechanisms that regulate the cell cycle will be performed in future to identify the protein target of compound 1, and future experiments on non-transformed epithelial cells will be performed to ensure the selectivity and specificity of the compounds [57].effect on cell adhesion was found, however, after the 48 h treatment, the very few cells present in the supernatant and the adherent cells were grouped together and no differences were observed between the diverse cell treatments in terms of apoptosis (Figure 8, lower panel).Overall, it is possible to assume that the growth inhibition induced by compound 1 on MDA-MB-231 cells, might be correlated to a cytostatic effect, rather than to a cytotoxic one.Thus, the exploration of the fine mechanisms that regulate the cell cycle will be performed in future to identify the protein target of compound 1, and future experiments on non-transformed epithelial cells will be performed to ensure the selectivity and specificity of the compounds [57].We investigated the effect of compound 1 in cell viability using the WST-1 tetrazolium salt assay.The stable tetrazolium salt WST-1 is cleaved to a soluble formazan by a mechanism that is largely dependent on the glycolytic production of NAD(P)H in viable cells, thus, the amount of formazan dye produced directly correlates with the number of metabolically active cells.We tested different concentrations of compound 1, and we found that the number of dead cell was not significantly different as compared to the control (Figure 7).Moreover, we investigated the effect of compound 1 on MDA-MB-231 cell cycle.We found that, starting from 10 µM concentration, this compound clearly induced a significant arrest of MDA-MB-231 cell cycle (Figure 8, upper panel).The effect on cell apoptosis was evaluated in the entire exposed cell population.As mentioned before, no significative effect on cell adhesion was found, however, after the 48 h treatment, the very few cells present in the supernatant and the adherent cells were grouped together and no differences were observed between the diverse cell treatments in terms of apoptosis (Figure 8, lower panel).Overall, it is possible to assume that the growth inhibition induced by compound 1 on MDA-MB-231 cells, might be correlated to a cytostatic effect, rather than to a cytotoxic one.Thus, the exploration of the fine mechanisms that regulate the cell cycle will be performed in future to identify the protein target of compound 1, and future experiments on non-transformed epithelial cells will be performed to ensure the selectivity and specificity of the compounds [57].The identification of the target responsible for the observed phenotype, above the many different growth factor signals and cyclin-dependent kinases will shed light in the understanding of tumor cell growth and progression [58,59].Moreover, further investigations will be attempted in view to characterize the signaling pathways underlying the biological effect [60][61][62], particularly investigating the role of caspases and the modulation of their phosphorylation [60,63,64].

General Methods
Analytical grade solvents and commercially available reagents were used without further The identification of the target responsible for the observed phenotype, above the many different growth factor signals and cyclin-dependent kinases will shed light in the understanding of tumor cell growth and progression [58,59].Moreover, further investigations will be attempted in view to characterize the signaling pathways underlying the biological effect [60][61][62], particularly investigating the role of caspases and the modulation of their phosphorylation [60,63,64].

General Methods
Analytical grade solvents and commercially available reagents were used without further purification.Flash column chromatography (FCC) purifications were performed manually using glass columns with Merck silica gel (0.040-0.063 mm, Merck, Darmstad, Germany), or using the Isolera system (Biotage, Uppsala, Sweden) and SNAP silica cartridges.TLC analyses were performed on Merck silica gel 60 F254 plates. 1 H-NMR and 13 C-NMR spectra were recorded on a Mercury 400 ( 1 H: 400 MHz, 13 C: 100 MHz), or a Gemini 200 ( 1 H: 200 MHz, 13 C: 50 MHz) instrument (Varian, Palo Alto, CA, USA).HSQC experiments were carried out to define the multiplicity of 13 C signals.
All chemical shifts are reported in parts per million (δ) referenced to residual nondeuterated solvent.Data are reported as follows: chemical shifts, multiplicity (br = broad, s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet; coupling constant(s) in Hz; integration).ESI mass spectra were carried out on a ion-trap double quadrupole mass spectrometer using electrospray (ES+) ionization techniques, and a normalized collision energy within the range of 25−32 eV for MSMS experiments.IR spectra were recorded with a FTIR-1600 spectrophotometer (Perkin-Elmer, Waltham, MA, USA).Elemental analyses were performed on a Perkin Elmer 240 C, H, N analyzer.Optical rotation measurements were performed on a DIP-370 polarimeter (JASCO Easton, MD, USA) and are given in 10 −1 deg•cm 2 •g −1 .

Cell Culture
Human MDA-MB-231 breast cancer cell lines are a model for aggressive, hormone-independent breast cancer.Cells were obtained from the American Tissue Culture Collection (Manassas, VA, USA) and maintained in DMEM (from Life Technologies, Carlsbad, CA, USA) containing 10% heat inactivated fetal calf serum (FCS, from Life Technologies) at 37 • C in a humidified atmosphere of 5% CO 2 /95% air.Cells were harvested from subconfluent cultures by incubation with a trypsin-EDTA solution, and propagated every 3 days at a ratio between 1:4 and 1:8.

Cell Growth Assay
MDA-MB-231 cells were plated at 5 × 10 4 cells/mL in 24-well plates under standard culture conditions.After 4h adhesion, cells were treated with either vehicle alone or different compounds.After 24 h or 48 h of treatments, cells were trypsinized, collected, and counted using a hemocytometer.

Cell Viability
Cell viability assay was performed using the WST-1 assay, a tetrazolium salt analog to MTT or XTT.Briefly, MDA-MB-231 cells were plated in in triplicate in a 96-well at 10-15 × 10 3 cells/mL density.After 4 h adhesion, cells were treated with different concentration of compound 1.Following 24 h or 48 h incubation, cells were exposed to 10 µL of the reagent directly to the cell cultures (200 µL final volume).The plates were incubated for 120 minutes at 37 • C in a humidified 5% CO 2 environment.The WST-1 formazan product was measured at 460 nm (reference wavelength of 630 nm) with an ELx800 Universal Microplate Reader from Bio-Tek Instruments Inc. (Winooski, VT, USA).

Cell Cycle Analysis
MDA-MB231 cells were plated at 5 × 10 5 cells/mL in 6-well plates under standard culture conditions.After 4 h adhesion, cells were treated with different concentration of compound 1.After 48 h cells were harvested by trypsinization, and then permeabilized with 70% ice-cold ethanol on ice for 30 min.Cells were then washed and incubated in staining buffer with 50 µg/mL propidium iodide (PI), 10 µg/mL RNase A and 0.1% Triton X-100 for 30 min in the dark.Subsequently, the cell cycle was analyzed by flow cytometry (FACSCan; BD Biosciences, San Jose, CA, USA).

Data Analysis
The results are expressed as mean ± standard deviation (SD).Differences in growth rates between groups were analyzed using the two-tailed t-test, statistical significance at p-values <0.05 were presented using respective symbols in the figure legends.

Conclusions
In recent years, small molecules drug discovery and development became a highly challenging field, and in this view we have identified potential novel drug compounds starting from library compounds and investigated their effect on a malignant human tumor cell line.Specifically, the application of a cell-based growth inhibition assay on a library of skeletally different glycomimetics allowed for the selection of a novel chemotype based on the hexahydro-2H-furo [3,2-b] [1,4]oxazine scaffold as a hit inhibitor of MDA-MB-231 cell growth.Subsequent follow-up synthesis of parent compounds and preliminary biological studies validated the selection of 1 as a valuable lead compound for the modulation of breast carcinoma cell cycle mechanism.
Although follow-up compounds showed reduced activity as compared to 1, interesting structure-activity analysis showed a quite specific structural requirement, revealing that both the three hydroxyl group and the Fmoc group are crucial for inhibition.Compound 1 clearly induced a significant arrest of MDA-MB-231 cell cycle, and we assume that the growth inhibition induced by compound 1 on MDA-MB-231 cells might be correlated to a cytostatic effect, whereas no significative effect on cell adhesion and apoptosis was found.
Although we have found a cytostatic effect on tumor cells, future experiments on non-transformed epithelial cells will be performed to ensure the selectivity and specificity of the compounds [57].The identification of the target responsible for the observed phenotype, above the many different growth factor signals and cyclin-dependent kinases will shed light in the understanding of tumor cell growth and progression [58,59].Moreover, further investigations will be attempted in view to characterize the signaling pathways underlying the biological effect [60][61][62], particularly investigating the role of caspases and the modulation of their phosphorylation [60,63,64].
Further biological tests will be carried out starting from flow cytometry profiling and the use of reverse chemical genetics approaches to have insight into more detailed fine mechanisms that regulate the cell cycle [39,68,69].

Figure 3 .
Figure 3. MDA-MB-231 cell number after 48 h of incubation with the selected molecules at 10 μM concentration.Values correspond to the mean of three independent experiments.Error bars indicate the corresponding standard deviations values.Student's t test was used to evaluate the data significance, * p < 0.05.

Figure 3 .
Figure 3. MDA-MB-231 cell number after 48 h of incubation with the selected molecules at 10 μM concentration.Values correspond to the mean of three independent experiments.Error bars indicate the corresponding standard deviations values.Student's t test was used to evaluate the data significance, * p < 0.05.

Figure 3 .
Figure 3. MDA-MB-231 cell number after 48 h of incubation with the selected molecules at 10 µM concentration.Values correspond to the mean of three independent experiments.Error bars indicate the corresponding standard deviations values.Student's t test was used to evaluate the data significance, * p < 0.05.

Figure 4 .
Figure 4. Control cells after 48 h (left), and cells after 48 h of incubation with compound 1 at 10 μM concentration (right).

Figure 5 .
Figure 5. Left: Cell growth inhibition in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration; Right: dose-response curve of log[1] vs. % cell growth normalized to control.Values indicate the mean of three independent experiments.Error bars show the corresponding standard deviation values.

Figure 4 .
Figure 4. Control cells after 48 h (left), and cells after 48 h of incubation with compound 1 at 10 µM concentration (right).

Figure 4 .
Figure 4. Control cells after 48 h (left), and cells after 48 h of incubation with compound 1 at 10 μM concentration (right).

Figure 5 .
Figure 5. Left: Cell growth inhibition in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration; Right: dose-response curve of log[1] vs. % cell growth normalized to control.Values indicate the mean of three independent experiments.Error bars show the corresponding standard deviation values.

Figure 5 .
Figure 5. Left: Cell growth inhibition in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration; Right: dose-response curve of log[1] vs. % cell growth normalized to control.Values indicate the mean of three independent experiments.Error bars show the corresponding standard deviation values.

Figure 4 .
Figure 4. Control cells after 48 h (left), and cells after 48 h of incubation with compound 1 at 10 μM concentration (right).

Figure 5 .
Figure 5. Left: Cell growth inhibition in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration; Right: dose-response curve of log[1] vs. % cell growth normalized to control.Values indicate the mean of three independent experiments.Error bars show the corresponding standard deviation values.

Table 2 .a
Cell growth inhibition of MDA-MB-231 cell line at 10 μM concentration after 48 h of incubation.Cell growth inhibition (% vs control) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values ± SD indicate the mean of three independent experiments.

Figure 7 .
Figure 7. Cell viability assay (WST-1) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values indicate the mean of three independent experiments and are expressed as percentage compared to control cells, error bars indicate the corresponding standard deviations values.

a
Cell growth inhibition (% vs control) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values ± SD indicate the mean of three independent experiments.

Figure 7 .
Figure 7. Cell viability assay (WST-1) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values indicate the mean of three independent experiments and are expressed as percentage compared to control cells, error bars indicate the corresponding standard deviations values.

a
Cell growth inhibition (% vs control) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values ± SD indicate the mean of three independent experiments.

Figure 7 .
Figure 7. Cell viability assay (WST-1) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values indicate the mean of three independent experiments and are expressed as percentage compared to control cells, error bars indicate the corresponding standard deviations values.

a
Cell growth inhibition (% vs control) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values ± SD indicate the mean of three independent experiments.

Figure 7 . 2 18
Figure 7. Cell viability assay (WST-1) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values indicate the mean of three independent experiments and are expressed as percentage compared to control cells, error bars indicate the corresponding standard

a
Cell growth inhibition (% vs control) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values ± SD indicate the mean of three independent experiments.

Figure 7 . 11 19
Figure 7. Cell viability assay (WST-1) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values indicate the mean of three independent experiments and are expressed as percentage compared to control cells, error bars indicate the corresponding standard

a
Cell growth inhibition (% vs control) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values ± SD indicate the mean of three independent experiments.

Figure 7 . 6 20
Figure 7. Cell viability assay (WST-1) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values indicate the mean of three independent experiments and are

Table 2 .a
Cell growth inhibition of MDA-MB-231 cell line at 10 μM concentration after 48 h of incubation.Cell growth inhibition (% vs control) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values ± SD indicate the mean of three independent experiments.

Figure 7 . 4 a
Figure 7. Cell viability assay (WST-1) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values indicate the mean of three independent experiments and are

4 a
Cell growth inhibition (% vs control) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values ± SD indicate the mean of three independent experiments.

Figure 7 .
Figure 7. Cell viability assay (WST-1) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values indicate the mean of three independent experiments and are expressed as percentage compared to control cells, error bars indicate the corresponding standard deviations values.

Figure 7 .
Figure 7. Cell viability assay (WST-1) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values indicate the mean of three independent experiments and are expressed as percentage compared to control cells, error bars indicate the corresponding standard deviations values.Molecules 2016, 21, 405 8 of 17

Figure 8 .
Figure 8. Cell cycle assay (upper panel) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values indicate the range of cell population percentage in different cell cycle phases of three independent experiments.Apoptosis assay (lower panel) in MDA-MB-231 cells after 48h of incubation with compound 1 at the reported concentration.Representative cell plot analysis of apoptosis in MDA-MB-231.

Figure 8 .
Figure 8. Cell cycle assay (upper panel) in MDA-MB-231 cells after 48 h of incubation with compound 1 at the reported concentration.Values indicate the range of cell population percentage in different cell cycle phases of three independent experiments.Apoptosis assay (lower panel) in MDA-MB-231 cells after 48h of incubation with compound 1 at the reported concentration.Representative cell plot analysis of apoptosis in MDA-MB-231.

Table 2 .
Cell growth inhibition of MDA-MB-231 cell line at 10 µM concentration after 48 h of incubation.

Table 2 .
Cell growth inhibition of MDA-MB-231 cell line at 10 μM concentration after 48 h of incubation.

Table 2 .
Cell growth inhibition of MDA-MB-231 cell line at 10 μM concentration after 48 h of incubation.

Table 2 .
Cell growth inhibition of MDA-MB-231 cell line at 10 μM concentration after 48 h of incubation.

Table 2 .
Cell growth inhibition of MDA-MB-231 cell line at 10 μM concentration after 48 h of incubation.

Table 2 .
Cell growth inhibition of MDA-MB-231 cell line at 10 μM concentration after 48 h of incubation.