Novel Triphenylantimony(V) and Triphenylbismuth(V) Complexes with Benzoic Acid Derivatives: Structural Characterization, in Vitro Antileishmanial and Antibacterial Activities and Cytotoxicity against Macrophages

Two novel organoantimony(V) and two organobismuth(V) complexes of the type ML2 were synthesized, with L = acetylsalicylic acid (HL1) or 3-acetoxybenzoic acid (HL2) and M = triphenylantimony(V) (M1) or triphenylbismuth(V) (M2). Complexes, [M1(L1)2] (1), [M1(L2)2]∙CHCl3 (2), [M2(L1)2], (3) and [M2(L2)2] (4), were characterized by elemental analysis, IR and NMR. Crystal structures of triphenylantimony(V) dicarboxylate complexes 1 and 2 were determined by single crystal X-ray diffraction. Structural analyses revealed that 1 and 2 adopt five-coordinated extremely distorted trigonal bipyramidal geometries, binding with three phenyl groups in the equatorial position and two deprotonated organic ligands (L) in the axial sites. The metal complexes, their metal salts and ligands were evaluated in vitro for their activities against Leishmania infantum and amazonensis promastigotes and Staphylococcus aureus and Pseudomonas aeruginosa bacteria. Both the metal complexes showed antileishmanial and antibacterial activities but the bismuth complexes were the most active. Intriguingly, complexation of organobismuth(V) salt reduced its activity against Leishmania, but increased it against bacteria. In vitro cytotoxic test of these complexes against murine macrophages showed that antimony(V) complexes were the least toxic. Considering the selectivity indexes, organoantimony(V) complexes emerge as the most promising antileishmanial agents and organobismuth(V) complex 3 as the best antibacterial agent.


Introduction
Bismuth and antimony compounds have been used in medicine for centuries [1,2].Bismuth is considered as the least toxic heavy stable metal and some bismuth(III) carboxylate derivatives, including bismuth subsalicylate and colloidal bismuth subcitrate, are clinically recommended drugs to treat various gastrointestinal diseases caused by the infection of Helicobacter pylori bacteria [3].
Despite of the fact that antimonials are highly noxious, they have been widely utilized in therapeutics for nearly a century as antiparasitic agents, especially in the treatment of leishmaniasis, a group of diseases caused by infection of protozoan parasites belonging to the genus Leishmania which are transmitted to humans by the bite of the infected female sandfly (genus Phlebotomus and Lutzomyia) [4].The modern era of therapeutic use of antimonials began in 1913, with the introduction of antimony(III) potassium tartrate (tartar emetic) in the treatment of leishmaniasis [5,6].About 70 years ago, trivalent antimonials were replaced by the less toxic pentavalent antimonials, such as meglumine antimoniate, in the treatment of all forms of the disease [7].Unfortunately, despite high cure rates, pentavalent antimonials are also associated with severe life-threatening side effects [8][9][10][11][12][13].Another major problem is the emergence of drug resistance that has reached epidemic proportions, particularly in parts of India [4,14].Other drugs are available, such as amphotericin B, miltefosine, paramomycin.However, all suffer some drawbacks, including side effects, high cost or drug resistance.
In the last two decades, several antimony and bismuth complexes have been synthesized and evaluated in preclinical assays, as an attempt to identify safer and more effective antileishmanial and antibacterial drugs.Interestingly, some bismuth complexes showed antileishmanial activity [15][16][17][18], whereas some antimony complexes exhibited antibacterial activity [19].
In this context, organometallic complexes of antimony and bismuth are of great interest due their chemically defined structure and the possibility of fine tuning both the activity and the toxicity of the compound through manipulation of the ligand and the organometallic moiety [17,[20][21][22].Organoantimony(III) complexes with different nitrogen donor heterocyclic ligands exhibited very high antileishmanial activity, in the nanomolar concentration range, but were also highly toxic to mammalian cells [22].Organoantimony(V) and organobismuth(V) complexes with lapachol showed moderate activity against leishmania amastigotes, the organoantimony(V) being more active and selective than organobismuth(V) complex [17].
A number of organoantimony(V) carboxylate complexes, with variation of carboxylate ligands and organometallic moieties, were screened for their activity against Leishmania major promastigotes and amastigotes, some of them (with m-or p-tolyl substituted benzoate ligands) showing good activities in the micromolar concentration range [21].Organoantimony(V) carboxylate complexes showed moderate antibacterial activity against Gibbereile zeae, Alternia solani, Rhizoctonia solani and Physolospora piricola [19], whereas tetraorganobismuth(V) aryloxyacetate complexes exhibited moderate to high antibacterial activity against P. aeruginosa and S. aureus [23].These microorganisms often express multidrug resistance and new chemotherapeutic compounds to treat and control infections by these pathogens have been extensively investigated [24].It is noteworthy that only few reports are available on the antileishmanial and antibacterial activities of organoantimony(V) carboxylate complexes [19,21] and, to the best of our knowledge, there is no report available on organobismuth(V) carboxylate complexes evaluated for their antileishmanial activity.The present paper reports the synthesis and characterization of two novel triphenylantimony(V) dicarboxylate and two triphenylbismuth(V) dicarboxylate complexes.The ligands are the benzoic acid derivatives, acetylsalicylic acid (HL1) and 3-acetoxybenzoic acid (HL2) (Figure 1).The metal precursors are triphenylantimony(V) dichloride (M1) and triphenylbismuth carbonate (M2) (Figure 1).The synthetic and analytical details for all complexes, and the crystal structure determination of the two antimony(V) complexes are described.The in vitro antileishmanial activities of the four complexes, their respective ligands (HL1-2) and metal precursors (M1-2) against L. infantum and L. amazonensis promastigotes, and their antibacterial activity against S. aureus Gram-positive and P. aeruginosa Gram-negative bacteria were evaluated.The complexes under study were also evaluated for their cytotoxicity towards murine peritoneal macrophages.

Characterization of Complexes 1-4
The organoantimony(V) and organobismuth(V) complexes were obtained in good yield and characterized by microanalysis and their FT-IR and NMR spectra.The crystal and molecular structures of complexes 1 and 2 were determined by X-ray crystallography Microanalysis and molar conductivity data suggested the formation of complexes of the ML 2 type where L = deprotonated ligand.The low molar conductivity ( M ) values measured for the complexes 1-4 [ M = 0.19 S.cm 2 mol −1 (1), 0.36 S.cm 2 mol −1 (2), 0.64 S.cm 2 mol −1 (3) and 0.36 S.cm 2 mol −1 (4)] indicated that the compounds are neutral in DMSO solution.For all complexes, the elemental analyses of C and H were obtained and were found to be in good agreement with the calculated values.In complex 2, one molecule of chloroform is present as a crystallization solvate.

Infrared Spectra
For carboxylic acids, broad bands profiles in the regions 3,500-3,000 cm −1 and 3,300-2,500 cm −1 are associated with the absence or presence of carboxylic acid dimer in solid state, respectively [25].In the IR spectra of free ligands, two typical profiles of bands for O-H stretching vibrations (υ-OH) were observed.In HL1 and HL2, the broad bands profile in the range 3,486-3,414 cm -1 and 3,078-2,546 cm -1 , respectively, correspond to υ-OH [26][27][28].In the IR spectra of the complexes, these bands disappeared indicating the deprotonation of ligands and coordination with metal through the oxygen of the carboxyl group.
The C=O bands occur in the range of 1,750-1,600 cm -1 [25,26].In the free carboxylic acids, these absorption bands corresponding to the asymmetric stretching vibration of the carboxyl C=O group occur at 1,692 cm -1 (HL1) [26,27] and 1,678 cm -1 (HL2) [28].In complexes 1-4, weakening of C=O bond occurred upon complexation which causes the lowering of wavenumber corresponding to asymmetrical carboxylate stretching absorbances.The value of Δν (νCO 2 − (asymm) − ν CO 2 − (symm) ) is comparatively less for all the complexes than for the free ligands suggesting the involvement of COO-group of carboxylate ligands in the coordination with [29][30][31][32] antimony or bismuth.The strong γ(CH) band at 732-744 cm -1 indicates the presence of the aryl group.No significant change was observed for the absorption bands at 1,754 cm −1 (HL1) and 1,752 cm −1 (HL1) corresponding to the stretching vibration of C=O of the ester, upon formation of complexes with Ph 3 Sb(V) or Ph 3 Bi(V).This indicates the noninvolvement of the ester oxygen atom in coordination to the metal.
The formation of Sb-O bonds is supported by the appearance of characteristic weak to medium intensity absorption bands in the region 452-478 cm -1 assigned to the Sb-O stretching mode [32].In addition, the frequencies corresponding to Sb-C deformations appear between 528 and 587 cm -1 , which is consistent with the literature [29][30][31][32].The Bi-C stretching frequency corresponding to y-mode was observed in the range 448-440 cm −1 as a medium to weak band [33].

NMR Spectra
The 1 H-NMR spectra of the complexes revealed the absence of the signal attributed to the carboxylic acid hydrogen (at 10.77 ppm and 11.14 ppm for HL1 and HL2, respectively) [26,34,35], indicating the deprotonation upon coordination to the metal.In addition, the resonances for the aromatic hydrogens were observed in the expected range 6.80-8.30ppm [21] with slight changes in the chemical shifts in comparison to the positions of these signals in the free ligands.In the spectra of all complexes, additional signals attributed to the hydrogens of the organometallic phenyl group were observed.On coordination no significant changes were observed for the resonances corresponding to the hydrogens of the methyl group, indicating that the ester oxygen is not attached to the metal center.
The 13 C-NMR spectra of both ligands showed the signal of the carboxylic acid carbon (C7) (see Figure 1) at 170.1 ppm and the aromatic carbons in the range of 122.2-151.3ppm.The signals attributed to the methyl carbon atom were observed at ~21.00 ppm.The 13 C-NMR spectra of the complexes showed small variances in the chemical shifts of the aromatic carbons (in the range of 122.9-160.3ppm) and carboxylic acid carbons (C7) resonances (0.68-1.93 ppm up field or downfield shift) compared with those observed for free ligands, which confirmed the coordination of metal to the oxygen atom of carboxylate group of the free ligands.No significant change was observed for the chemical shift corresponding to the methyl carbon in the complexes, which confirmed the noninvolvement of the methyl carbon in coordination. 13C-NMR signals due to carbons of the organometallic phenyl group appeared in the range of 129.5-137.7 ppm (1 and 3) and 130.5-159.8ppm (2 and 4).The attributions of H and C resonances in the 1 H-and 13 C-NMR spectra of complexes 1-4 were confirmed by 2D NMR experiments ( 1 H 1 H-COSY, 1 H 13 C-HMQC and 1 H 13 C-HMBC).To exemplify, the 2D NMR HMQC spectrum obtained for complex 3 is shown in Figure 2 [see Supplementary Figures S1-S4 for the 1 H and 13 C spectra of HL1 and complex 3].Colorless crystals of 1 and 2 were obtained from slow evaporation in a chloroform-diethyl ether mixture, while brown crystals of 3 were obtained by the slow evaporation of its dichloromethane solution (see Experimental Section).The solid-state structures of the crystals were determined by single crystal X-ray diffraction (Mo Kα radiation, λ = 0.71073 Å, graphite monochromater).The crystal originated from 1 crystallized in monoclinic system with half molecule of complex per asymmetric unit.The crystal originated from 2 crystallized in triclinic system with one molecule of the complex and one molecule of CHCl 3 solvent per asymmetric unit.The crystal data obtained for 3 (not described here) was similar to that described by Sharutin et al. [36], and showed that the ligands are bound to the metal center, hence, confirmed the infrared analysis.Crystallographic data and experimental details of the structure determinations for 1 and 2 are listed in Table 1.The molecular plots for 1 and 2 are shown in Figure 3. Selected bond lengths (Å) and angles (°) for 1 and 2 are listed in Table 2.As illustrated in Figure 3, the molecular structure of both complexes 1 and 2 are similar in relation to the coordination sphere around the central antimony atom.The antimony atom in these complexes is five-coordinated with extremely distorted trigonal bipyramidal geometry.The formally two unidentate carboxylate ligands sit in the axial positions with some slight variance in the internal bond lengths (Table 2) while three carbon atoms of the three phenyl groups occupy the equatorial plane.For complex 1, the antimony, located in the crystallographic two-fold symmetry axis, and the equatorial carbon atoms are in the same plane.For complex 2, the antimony and the equatorial carbon atoms are close to the plane 1, through Sb(1), C (21), C (31), and (C41).Sb is the most distant atom from this plane, with d[Sb − plane] = 0.053(1) Å.The carbon atoms are in the opposite side of the plane, if compared to antimony.
Considering that these complexes present a coordination number of 5, the tau parameter was calculated for these complexes to measure the grade of distortion.The tau (τ) parameter is defined as (beta-alpha)/60) [where beta = the first largest angle and alpha = the second largest angle in the coordination sphere] and its value varies from 0 (in regular square-based pyramidal geometry) to 1 (in regular trigonal bipyramidal geometry) [40].In complexes 1 and 2 the calculated tau (τ) values were 0.38 and 0.47, respectively.The tau (τ) value less than 0.5 demonstrate that in both complexes, the geometry around the antimony atom is extremely distorted trigonal bipyramidal, suggesting a distortion towards the square-based pyramidal arrangement.
The reason behind this distortion could be the presence of the C=O of carboxylate group oriented in the direction of axial axis causing repulsion of phenyl groups in the equatorial position.Furthermore, it is important to highlight that the antimony atom has Lewis-acid character which favors the interaction with electron donor species as the oxygen atom of C=O of carboxylate group.In fact, the Sb⋯O(=C) distances observed in 1 [Sb1⋯O2 = Sb1⋯O2 i = 2.668 Å] and in 2 [Sb1⋯O2 = 2.728 Å, and Sb1⋯O12 = 2.712 Å], are approximately 0.5-0.6Å longer than for the primary carboxylate coordination site but they are significantly smaller than the sum of the van der Waals radius of Sb and O atoms (ca.3.5 Å) [21].
These data indicate that Sb atom may have weak interaction with the carboxylate C=O oxygen atom.In this case, it would be more accurate to consider these two new interactions in calculating the coordination number of antimony(V) atom in the complexes.So, the probable geometry around Sb(V) would be distorted pentagonal bipyramidal, as known for various other triphenylbismuth(V) complexes [36].

Biological Activity
All complexes 1-4 along their metal salts M1-2 and the free ligands HL1-2 were tested in vitro for their antileishmanial activity against L. amazonensis and L. infantum promastigotes and also for their antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa bacterial strains.
L. infantum is the causative agent for visceral leishmaniasis [41] while L. amazonensis is related to the cutaneous form of the disease in the New World [42].Since these two species of Leishmania are the etiological agents of different clinical manifestations, they might also differ metabolically which reflect in their distinct drug sensitivity.This observation highlights the importance to use different Leishmania species for drug prospecting screening [43].
In bacterial pathogens, antimicrobial drug resistance is of health concern worldwide [44,45].The major driving force behind the spread of resistance is the extensive use of antimicrobial agents, but the nature of this relationship is complex [46].P. aeruginosa and S. aureus are two problematic nosocomial pathogens; both often express multidrug resistance.Therefore, new chemotherapeutic compounds to treat and control infections by these microorganisms have been broadly studied and developed [24].In this study, P. aeruginosa and S. aureus were selected as the representative strains for Gram-negative and Gram-positive bacteria, respectively.
All compounds were also tested for their cytotoxic activity against mouse peritoneal macrophages.Macrophages are used here as a model of mammalian cells towards which drugs may exert toxicity.It also represents the host cell in which leishmania parasite develops.

Anti-Leishmanial Activity against Promastigotes
The four complexes, their metal salts and their respective free ligands were primarily tested for their activity to investigate whether they inhibit the growth of L. amazonensis and L. infantum promastigotes.The growth rate of the promastigotes incubated for 48 h in the presence of test compounds was compared to those of nontreated negative control and to those treated with the standard antileishmanial antimony(III) drug tartar emetic.
As shown in Table 3, both the organoantimony(V) and organotbismuth(V) complexes were found to be active against Leishmania strains.However, the antimony(V) complexes (IC 50 in the range of 8.9-30.7 μM) were less effective than bismuth(V) complexes (IC 50 range, 2.9-8.7 μM).The complexes synthesized from acetylsalicylic acid also tended to be less active than those synthesized from 3-acetoxybenzoic acid.Interestingly, the ligands showed no intrinsic antileishmanial activity, contrary to the organometallic salts that were at least as effective as their respective complexes.This data strongly suggest that the organometallic salts are responsible for the antileishmanial activity of the complexes and that the complexes must dissociate in order to exert their activity.It also suggests that the activity of these complexes is mediated by the complexation of organoantimony(V) or organobismuth(V) with biomolecule(s), leading to cell death.While these biomolecules still remain to be identified, intracellular thiols and nucleosides are potential molecular targets, considering their involvement in the mechanism of action of conventional antimonial drugs [47].
The fact that the organobismuth(V) complexes were less effective than the organobismuth salt, in contrast to the organoantimony(V) complexes that were mostly as effective as the organoantimony salt, suggests a difference in the stability of the organobismuth(V) complexes, compared to the organoantimony(V) complexes.The lower activity of acetylsalicylic acid complexes in comparison to the respective 3-acetoxybenzoic acid ones also suggests a greater stability of the former.
Intriguingly, when compared to the trivalent antimonial drug tartar emetic, the pentavalent organoantimony complexes were found to be more active against Leishmania promastigotes.It is also noteworthy that pentavalent antimonial drugs such a meglumine antimoniate [47] are inactive in the promastigotes model.The differential activity of these organoantimonial compounds, when compared to the conventional pentavalent antimonial drugs may be explained in part by the more lipophilic character of the former and their higher permeability across the parasitic plasma membrane.Another explanation may be a different metabolism and a distinct mechanism of action.

Antibacterial Activity
Table 4 shows the minimum inhibitory concentrations against the growth of Gram-positive bacteria S. aureus (ATCC 6538) and Gram-negative bacteria P. aeruginosa (ATCC 27853) for the four complexes, their metal salts and the free ligands together with the values obtained for tetracycline as a positive control.All complexes were active against both bacterial strains but the organobismuth(V) complexes were at least ~8-times more active than the antimony(V) complexes.As expected the free ligands were not active against bacteria.On the other hand the organometallic salt precursors showed antibacterial activities against both strains, the bismuth being more effective than the antimony salt.It is noteworthy that complexes were more effective than their respective organometallic salt, indicating that complexation of the organometal resulted in improved antibacterial activities.Interestingly, an opposite tendency was observed in the antileishmanial assay, where the organometal precursors were more active than the respective complexes.
As a possible explanation for the greater effectiveness of the complexes, the ligand may enhance the uptake of the drug by the bacterial cells.Strikingly, organobismuth(V) complexes 3 and 4 showed nearly the same activity as the standard tetracycline drug against the Gram-positive strain.These complexes were even more effective than tetracycline against the Gram-negative strain.

Cytotoxicity against Mammalian Cells
The in vitro cytotoxicity of the complexes, along their metal salts and ligands, was evaluated by means of the MTT assays towards mouse peritoneal macrophages.The results of in vitro cytotoxic activity are expressed as CC 50 , which is the concentration of compound that inhibits the viability of macrophages by 50% as compared to untreated macrophages control.
Table 5 shows that organoantimony(V) complexes were about 10-fold less cytotoxic than the organobismuth(V) complexes.This makes the antimony(V) complexes more selective than the bismuth(V) analogues against the Leishmania strains (SI in the range of 2.5-4.5 for antimony(V) complexes).The ligands showed very low cytotoxicity, with 10-to 100-fold greater CC 50 values than those of the complexes.Interestingly, the antimony(V) complexes were slightly less cytotoxic than the corresponding metal salt in contrast to the bismuth(V) complexes which showed higher cytotoxicities.The complexes synthesized from acetylsalicylic acid were also less cytotoxic than those obtained from 3-acetoxybenzoic acid.

Table 5.
In vitro cytotoxicity of complexes 1-4, metal salts Ph 3 SbCl 2 and Ph 3 BiCO 3 , and the free ligands acetylsalicyclic acid (HL1) and 3-acetoxybenzoic acid (HL2) against mouse peritoneal macrophages.* CC 50 : concentration that is cytotoxic against 50% of macrophages; SI: selective index; a calculated as the ratio between CC 50 in murine macrophages and IC 50 in L. infantum; b calculated as the ratio between CC 50 in murine macrophages and IC 50 in L. amazonensis (WT); c calculated as the ratio between CC 50 in murine macrophages and MIC in Pseudomonas aureginosa; and d calculated as the ratio between MIC in murine macrophages and MIC in Staphylococcus aereus.
Regarding the potential of these complexes as antibacterial agents, the organobismuth(V) complex with acetylsalicylic acid (complex 3) was found to be the most selective compound especially against the Gram-positive strain S. aureus.

Material and Methods
All solid reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA), Merck (Darmstadt, Germany) or Synth (São Paulo, Brasil) and used without further purification.Melting points were determined on Mettler FT80, with a heating rate 4 °C/min and are uncorrected.Elemental analyses were performed on a Perkin-Elmer PE-2400 CHNS analyzer.Infrared spectra were recorded in the 4000-400 cm -1 range with samples in KBr pellets using a Perkin-Elmer RX-83303 equipment.Conductivity data were obtained on Digimid DM31 Conductivimeter, equipped with conductivity cell with a 3 mL capacity and constant equal to 1.185 cm −1 .Measurements were taken at 25 °C.The concentration of each complex in DMSO solution was approximately 10 -3 mol L -1 .Standard solutions of NaCl, KCl, Ba(NO) 3 and (NH) 4 Cr 2 O 7 were prepared in the same concentrations.The electronic spectra were registered on a Varian ® Cary 100 UV-Visible Spectrophotometer, in the 200-800 nm range, in 10% DMSO/Water solution at room temperature.The spectra of the complexes were different from those of free ligands and did not show significant changes as a function of time.For all complexes, 1D ( 1 H, 13 C, DEPT 135) and 2D ( 1 H 1 H-COSY, 1 H 13 C-HMBC, and 1 H 13 C-HMQC) NMR spectra in deuterated chloroform (CDCl 3 ) were obtained on a Bruker Advance DPX-400 (400 MHz) spectrometer. 1 H-and 13 C-NMR chemical shifts were measured relative to tetramethylsilane (TMS) and CDCl 3 as internal reference.Single crystal X-ray diffraction data for complexes 1 and 2 were obtained on an Xcalibur Atlas Gemini Ultra diffractometer (LabCri-UFMG), using graphite monochromated Mo-Kα radiation (λ = 0.71073 Å).Final unit cell parameters and the integration of the collected reflections were performed using the CrysAlisPro software [49] (Version 1.171.33.55 release 05-01-2010 CrysAlis171.NET).The structure solutions and full-matrix least squares refinements based on F 2 were performed with the SHELXS-97 [50] and SHELXL-97 [51] program package.All atoms except hydrogen were refined anisotropically.Although many hydrogen atoms could be identified in a Fourier difference map, all of them were geometrically added to the structure and then refined by the riding model in the final stages.The crystallographic data were deposited at Cambridge Crystallographic Data Center under CCDC 981904 (1) and CCDC 981905 (2).For the syntheses of complexes 1 and 2, the free carboxylic acid ligand acetylsalicylic acid (HL1) or 3-acetoxybenzoic acid (HL2) (0.360 g, 2.0 mmol) was dissolved in dry methanol (30 mL).Sodium salts of the ligands were obtained by adding sodium methoxide (0.108 g, 2.0 mmol) directly into the ligand solution and stirring for 1-2 h in a Schlenk flask, under nitrogen environment at room temperature.The organometallic precursor Ph 3 SbCl 2 (0.424 g, 1.0 mmol) was added to the solution and stirred for 24 h under nitrogen environment at room temperature.All the suspended particles in the solution were filtered-off and from the transparent colorless solution obtained, solvent was removed in vacuum to dryness.The dry solid obtained was dissolved in chloroform (40 mL) and insoluble NaCl was filtered-off.The clear transparent filtrate was concentrated in vacuum to ca 5-10 mL.Bis- (3-acetoxybenzoato)  , H9), (COOH of free ligand) = disappeared in complex. 13C-NMR (100 MHz, CDCl 3 , 27 °C): δ: 171.5 (O=C-CH 3 , C8), 169.4 (C=OO-, C7), 160.3 (Ar-C, C3), 134.9 (Ar-C, C1), 134.1 (Ar-C of Ph 3 Sb, C2'), 131.4 (Ar-C of Ph 3 Sb, C3'), 130.9 (Ar-C of Ph 3 Sb, C4'), 128.9 (Ar-C, C5), 127.6 (Ar-C, C6), 124.9 (Ar-C, C4), 123.1 (Ar-C, C2), 21.1 (O=C-CH 3 , C9).

Syntheses of Triphenylbimsuth(V) Complexes 3 and 4
In the syntheses of organobismuth(V) complexes 3 and 4, the ligand HL1 or HL2 (0.360 g, 2.0 mmol) was dissolved in dichloromethane (30 mL) and the organometallic precursor Ph 3 BiCO 3 (0.5 g, 1.0 mmol) was added directly into the ligand solution.The reaction mixture was stirred for 24 h in Schlenk flask under nitrogen environment at room temperature.The solution was then filtered to remove the suspended particles and the clear transparent filtrate was concentrated in vacuum to ca 15-20 mL. Brown crystals were obtained for complex 3 -[bis-(acetylsalicylato)triphenylbismuth(V)] while complex 4 -[bis-(3-acetoxybenzoato)triphenyl-bismuth(V)] was obtained as a dry brown powder by slow evaporation of a dichloromethane solution.The synthetic procedures are shown in Scheme 1.

Sthaphyloccocus aureus [ATCC6538] and Pseudomonas aeruginosa [ATCC27853] stored in
Müller-Hilton broth were sub-cultured for testing in the same medium and incubated for 24 h, respectively, at 37 °C.The bacterial cells of the two strains were suspended in Müller-Hilton broth to produce inocula of 10 8 CFU mL -1 , determined by a spectrophotometric method.Serial dilutions of the compounds, previously dissolved in DMSO, were prepared in 10 test tubes with Müller-Hilton broth to final concentrations from 100 μg mL -1 to 0.20 μg mL -1 for P. aeruginosa and S. aureus.An old inoculum of each strain was added to the tubes to obtain final concentrations of 10 5 CFU.MIC, the lowest concentration to give 100% inhibition of growth, was determined visually after incubation for 24 h at 37 °C.Tests using tetracycline as positive control and DMSO as negative control were carried out in parallel.All tests were performed in triplicate with full agreement between the results.

Cytotoxicity Assay against Mouse Peritoneal Macrophages
The concentration of studied compounds that is cytotoxic to 50% of the macrophages (CC 50 ) was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) method according to Mosmann [57].Briefly, peritoneal macrophages were obtained from Balb/c mice after intraperitoneal injection of 2 mL of 3% sodium thioglycolate medium.Mice were euthanized after 72 h, just before removal of peritoneal macrophages by washing with cold RPMI 1640 medium.The procedure was approved by the Internal Ethics Committee in Animal Experimentation (CEUA) of the Federal University of Uberlândia (Protocol 069/2013).After washing, the cell suspension (4.0 × 10 6 /mL) was seeded (0.1 mL) in 96-well flat bottom plates.Macrophages were allowed to adhere for 2 h and non-adherent cells were removed by washing with RPMI.Then, the compounds were added to the wells at different concentrations and the cells were further cultured in RPMI-1640 supplemented with 10% FBS for 24 h at 37 °C in a humidified 5% CO 2 atmosphere.Thereafter, the medium was replaced with fresh RPMI containing 0.5 mg/mL of MTT and the plates were incubated for additional 4 h.Supernatants were aspirated and the formazan crystals formed were dissolved in 100 μL of DMSO.After 15 min of incubation at room temperature, absorbance of solubilized MTT formazan product was spectrophotometrically measured at 570 nm.

Statistical Analysis
The IC 50 and CC 50 values were calculated by nonlinear regression using the software GraphPad Prism 5.0.The acceptable level of significance was 95% (p < 0.05).

Conclusions
In the present study, two new organoantimony(V) and two organobismuth(V) derivatives containing acetylsalicylic or 3-acetoxybenzoic acid were synthesized, characterized and evaluated for their in vitro antileishmanial, antibacterial and cytotoxic activities.Single X-ray diffraction analyses of the Sb(V) complexes showed that the central antimony atom is five-coordinated and adopted extremely distorted trigonal bipyramidal geometries.Both the organometallic complexes showed antileishmanial and antibacterial activities but the bismuth complexes were the most active.Intriguingly, complexation of the organobismuth(V) salt reduced its activity against Leishmania, whereas it increased its antibacterial activity.Evaluation of the in vitro cytotoxic activity of these complexes against murine macrophages showed that the antimony(V) complexes were less cytotoxic than the bismuth(V) derivatives.Considering the selectivity indexes, the organoantimony(V) complexes emerge as the most promising antileishmanial agents and organobismuth(V) complex 3 as the best antibacterial agent mainly against Gram-positive S. aureus bacterial strain.

Table 1 .
Crystallographic data and structural refinement parameters for complexes 1 and 2.