Antibacterial and EGFR-Tyrosine Kinase Inhibitory Activities of Polyhydroxylated Xanthones from Garcinia succifolia

Chemical investigation of the methanol extract of the wood of Garcinia succifolia Kurz (Clusiaceae) led to the isolation of 1,5-dihydroxyxanthone (1), 1,7-dihydroxyxanthone (2), 1,3,7-trihydroxyxanthone (3), 1,5,6-trihydroxyxanthone (4), 1,6,7-trihydroxyxanthone (5), and 1,3,6,7-tetrahydroxyxanthone (6). All of the isolated xanthones were evaluated for their antibacterial activity against bacterial reference strains, two Gram-positive (Staphylococcus aureus ATTC 25923, Bacillus subtillis ATCC 6633) and two Gram-negative (Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853), and environmental drug-resistant isolates (S. aureus B1, Enteroccoccus faecalis W1, and E. coli G1), as well as for their epidermal growth factor receptor (EGFR) of tyrosine kinase inhibitory activity. Only 1,5,6-trihydroxy-(4), 1,6,7-trihydroxy-(5), and 1,3,6,7-tetrahydroxyxanthones (6) exhibited antibacterial activity against Gram-positive bacteria, however none was active against vancomycin-resistant E. faecalis. Additionally, 1,7-dihydroxyxanthone (2) showed synergism with oxacillin, but not with ampicillin. On the other hand, only 1,5-dihydroxyxanthone (1) and 1,7-dihydroxyxanthone (2) were found to exhibit the EGFR-tyrosine kinase inhibitory activity, with IC50 values of 90.34 and 223 nM, respectively.


Introduction
Many plants of the genus Garcinia (Family Clusiaceae) have been used in traditional medicine in several parts of the world for treatment of the most different illnesses [1]. In Thailand, where twenty-nine species have been observed [2], G. mangostana, G. speciosa and G. cowa have been widely used in Thai folk medicine. For example, the fruit hull of G. mangostana is used for healing of skin infections and wounds [3], whereas the bark and heartwood of G. speciosa are used for treatment of blood disorders, and its pericarp is used for the treatment of diarrhea [4]. Additionally, the bark, latex and root of G. cowa are used as an antifever agent while its fruits and leaves are used for indigestion, improvement of blood circulation and also as an expectorant [5,6]. The plants of this genus are also a rich source of xanthones [7], many of which exhibit interesting biological and pharmacological activities such as antibacterial [8,9] and anticancer properties [10]. As part of our ongoing research on the search for anticancer agents and antibiotics from natural sources, and since there are no previous reports on the chemical components and biological activity of G. succifolia, we have investigated its chemical constituents to evaluate their antibacterial activity against bacterial reference strains and multidrug-resistant isolates, as well as their inhibitory activity against the tyrosine kinase of epidermal growth factor receptor (EGFR). Examination of the methanol crude extract of the wood of G. succifolia Kurz, collected from Northern Thailand, led to the isolation of six polyhydroxylated xanthones: 1,5-dihydroxyxanthone (1), 1,7-dihydroxyxanthone (2), 1,3,7-trihydroxyxanthone (3), 1,5,6-trihydroxyxanthone (4), 1,6,7-trihydroxyxanthone (5), and 1,3,6,7-tetra-hydroxyxanthone (6) ( Figure 1). All the xanthones were tested against bacterial reference strains: Gram-positive (Staphylococcus aureus ATCC 25923 and Bacillus subtillis ATCC 6633) and Gram-negative (Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853), Candida albicans ATCC 10231, as well as against multidrug-resistant bacteria isolated from the environment. Furthermore, the potential synergism between these xanthones and antibiotics was also evaluated against multidrug-resistant bacteria: methicillin-resistant S. aureus (MRSA) and vancomycin-resistant enterococci (VRE). Xanthones 1-6 were also tested for their inhibitory activity against the EGFR protein tyrosine kinase, an important protein target for antitumor drug discovery.

E. coli G1
Xanthones 1-6 were also tested for their capacity to inhibit the EGFR protein tyrosine kinase activity since EGFR has been found to involve several types of cancer such as ovary, prostrate, breast, lung, colon, head and neck cancer [22], and it is also the target of an expanding class of anticancer therapies such as gefitinib (Iressa TM , AstraZeneca, London, UK) [23] and erlotinib (OSI-774, Tarceva TM , OSI Pharmaceuticals, Northbrook, IL, USA) for lung cancer [24], and trastuzumab (Herceptin TM , Genentech, South San Francisco, CA, USA) for breast cancer [25]. The results (Table 4) show that, in the presence of 0.01 µM of 1,5-dihydroxyxanthone (1) and 1,7-dihydroxyxanthone (2), the relative activity of EGFR-tyrosine kinase was found to decrease to 19.01% and 51.31%, respectively, when compared to the control. In the presence of 1 µM of gefitinib, a standard EGFR-tyrosine kinase inhibitor, the activity of this enzyme was reduced to 32.65%. Thus, in comparison to gefitinib (IC50 = 33 nM) [26], 1,5-dihydroxyxanthone (1) (IC50 = 90.34 nM) and 1,7-dihydroxyxanthone (2) (IC50 = 223 nM) ( Table 4) can be considered promising EGFR-tyrosine kinase inhibitors. Since xanthones 3-6 did not show significant inhibitory activity against the EGFR-tyrosine kinase, their IC50 were not determined. Examination of the structural features of xanthones 1-6 led to a suggestion that the EGFR prefers to bind the dihydroxyxanthones to the tri-and tetrahydroxy counterparts. Moreover, the positions of the hydroxyl groups also seem to play an important role in the activity of the polyhdroxylated xanthones since the hydroxyl groups on positions 3 and 6 were found to reduce their inhibitory activity, while the hydroxyl groups on positions 2 and 5 were found to increase it. To our knowledge, this is the first report on inhibitory activity of xanthones against the EGFR protein tyrosine kinase and consequently, dihydroxyxanthones can represent a new class of EGFR protein tyrosine kinase inhibitors.

General Experimentation Procedures
Melting points were determined on a Bock monoscope (Bibby Sterilin, Stratfordshire, UK) and are uncorrected. Infrared spectra were recorded on an ATI Mattson Genesis Series FTIR TM (Madison, WI, USA) using WinFIRST Software. 1 H and 13 C-NMR spectra were recorded at ambient temperature on a Bruker AMC instrument operating at 300. 13

Bacterial Strains
For the antimicrobial assays, xanthones 1-6 were tested against bacterial reference strains, two Gram-positive (Staphylococcus aureus ATCC 25923 and Bacillus subtilis ATCC 6633) and two Gram-negative (Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853) bacteria; Candida albicans ATCC 10231; multidrug-resistant bacteria isolated from the environment, S. aureus B1 (isolated from a public bus), Enterococcus faecalis W1 (isolated from river water) and E. coli G1 (isolated from seagull feces). Bacteria were grown in Mueller-Hinton agar (MH-BioKar diagnostics, Allonne, France) from stock cultures, while C. albicans was grown in Sabouraud dextrose agar (SAB-BioKar Diagnostics, Allonne, France). MH and SAB plates were incubated at 37 °C prior to obtain fresh cultures for each in vitro bioassay.

Determination of Minimum Inhibitory and Bactericidal/Fungicidal Concentrations
The minimum inhibitory concentrations (MIC) of xanthones 1-6 were determined using a broth microdilution technique, following the recommendations of the Clinical and Laboratory Standards Institute [27]. Stock solutions of 10 mg/mL (prepared by dissolving each xanthone in dimethylsulfoxide (DMSO-Applichem GmbH, Darmstadt, Germany) were serially diluted in Mueller-Hinton broth (MHB-BioKar Diagnostics, Allonne, France) to achieve in-test concentrations ranging from 2 to 256 µg/mL. Ciprofloxacin in the concentration range from 0.03125 to 16 μg/mL was used as control drug in the experiment. Each bacterial inoculum was prepared in MHB, while C. albicans inoculum was prepared in RPMI-1640 with L-glutamine, with MOPS and without NaHCO3 (Lonza, Walkersville, MD, USA). All inocula were standardized in order to obtain a concentration of 5 × 10 5 CFU/mL in each inoculated well of the microtiter plate. The concentration of DMSO in the highest in-test concentration did not affect the microbial growth. The MIC was defined as the lowest concentration of xanthone that inhibited the visible growth. The minimum bactericidal/fungicidal concentration (MBC/MFC) was determined by spreading 10 µL on MH/SAB plates from the sample showing no visible growth, with further incubation for 24 h at 37 °C; the lowest concentration at which no growth occurred on MB/SAB plates was defined as the MBC/MFC, respectively.

Screening of Combined Effect between Xanthones and Antibiotics
A screening susceptibility test to assess the combined effect between the xanthones 1-6 and antibiotics was conducted using the disc diffusion method on MH, according to the procedure already described by Gomes et al. [13]. Briefly, multidrug-resistant isolates were picked from overnight cultures in MH, and suspensions were prepared in buffered peptone water (Oxoid, Basingstoke, England) by adjusting the turbidity to equal a 0.5 McFarland standard. A set of antibiotic discs (Oxoid) was selected based on the resistance of the isolates towards these antibiotics. Antibiotic discs alone (controls) and impregnated with 15 µL of a 1 mg/mL solution (in DMSO) of each xanthone were placed on the agar plate seeded with the respective bacteria. Fifteen microliter of DMSO impregnated in a sterile filter paper disc (6 mm in diameter) (Oxoid) was used as a negative control. Inoculated MH plates were incubated overnight at 37 °C. Each xanthone was tested in duplicate. Potential synergism was recorded when the halo of antibiotic discs impregnated with metabolites was greater than the halo of antibiotic discs or xanthone-impregnated disc alone.

Synergy Test: Checkerboard Method
Based on the results of the previous assay, potential synergy between 1,7-dihydroxyxanthone (2) and oxacillin or ampicillin (Sigma-Aldrich, St. Louis, MO, USA) against MRSA (S. aureus B1), and vancomycin against VRE (E. faecalis W1) was checked using a broth microdilution checkerboard method, as already described [13]. Briefly, the stock solutions and serial two-fold dilutions of the tested xanthone and antibiotic to at least double the MIC were prepared according to the recommendations of CLSI [27]. The xanthone to be tested was serially diluted along the ordinate, while the antibiotic was diluted along the abscissa. A bacterial inoculum equal to a 0.5 McFarland turbidity standard was prepared in MHB. Each microtiter plate well was inoculated with 100 µL of a bacterial inoculum of 5 × 10 5 CFU/mL, and the plates were incubated overnight at 37 °C. Two independent experiments in duplicate were performed. The fractional inhibitory concentration (FIC) was calculated as follows: FIC of drug A (FIC A) = MIC of drug A in combination/MIC of drug A alone, and FIC of drug B (FIC B) = MIC of drug B in combination/MIC of drug B alone. The FIC index (ΣFIC), calculated as the sum of each FIC, was interpreted as follows: ΣFIC ≤ 0.5, synergy; 0.5 < ΣFIC ≤ 4, no interaction; 4 < ΣFIC, antagonism [28].

Tyrosine Kinase Inhibition Assay
Xanthones 1-6 were dissolved in 100% dimethyl sulfoxide (DMSO) to a concentration of 35 µM, and used as stock solution. The working solution was prepared by diluting with 1× kinase buffer containing 2 mM DTT to concentration of 60 nM. Samples were kept at 4 °C prior to use. Tyrosine kinase inhibition assays by fluorescence were performed according to the manufacturer's instruction. Briefly, 12.5 µL of kinase sample was incubated with 1.25 µL of the compound at room temperature for 5 min. Next, add 20 µL of Antibody Beacon detection complex plus substrate, and incubated at room temperature for 10 min in the dark. Subsequently, start the reaction by adding ATP with a final concentration of 0.5 mM/reaction, and the activities were determined using fluorescence microplate reader with excitation/emission at 485/535 nm.

Determination of IC50
Xanthones 1-6 were diluted in two-fold dilution (final concentration ranging from 0.975 nM to 500 nM), and 12.5 µL of the solution was added to 12.5 µL of the kinase sample, incubated at room temperature for 5 min. Next add 20 µL of Antibody Beacon detection complex plus substrate, and incubated at room temperature for 10 min. Subsequently, start the reaction by adding ATP with a final concentration of 0.5 mM/reaction. The activities were determined using fluorescence microplate reader with with excitation/emission at 485/535 nm. Non-linear regression dose response curves were plotted against activity and log inhibitor concentration. The fifty percent inhibitions (IC50) were calculated using the GraphPad Prism program (GraphPad Software Inc., San Diego, CA, USA).

Conclusions
The results obtained from this study revealed that polyhydroxylated xanthones are potential antibacterial agents, especially against the multidrug-resistant Gram-positive bacteria. Although, this study did not allow us to draw any conclusions about the structure activity relationship of the polyhydroxylated xanthones, it seems that the number and position of the hydroxyl groups on the xanthone nucleus can influence their antibacterial activity as well as their synergistic effect with antibiotics. With the emergence of multidrug-resistant organisms, combining natural products, such as xanthones, with antibiotics can be considered a useful approach to combat resistant bacteria. On the other hand, the capacity of the dihydroxyxanthones, namely 1,5-dihydroxyxanthone (1) and 1,7-dihydroxyxanthone (2) to inhibit the activity of EGFR protein tyrosine kinase, an important target of anticancer chemotherapies, can be considered as a support for polyhydroxylated xanthones as potential chemopreventive agents. However, further investigation of these xanthones on human cancer cell lines is necessary to support this hypothesis.