Antidiabetic and Antioxidant Properties of Alkaloids from Catharanthus roseus (L.) G. Don
Soon Huat Tiong 1, Chung Yeng Looi 2, Hazrina Hazni 1, Aditya Arya 2,3, Mohammadjavad Paydar 2, Won Fen Wong 4
, Shiau-Chuen Cheah 5, Mohd Rais Mustafa 2 and Khalijah Awang 1,*
, Shiau-Chuen Cheah 5, Mohd Rais Mustafa 2 and Khalijah Awang 1,*
1
Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
2
Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
3
Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
4
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
5
Faculty of Medicine and Health Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, Cheras 56000 Kuala Lumpur, Malaysia
*
Author to whom correspondence should be addressed.
Molecules 2013, 18(8), 9770-9784; https://doi.org/10.3390/molecules18089770
Received: 17 June 2013 / Revised: 2 August 2013 / Accepted: 9 August 2013 / Published: 15 August 2013
(This article belongs to the Section Natural Products Chemistry)
Abstract
Catharanthus roseus (L.) G. Don is a herbal plant traditionally used by local populations in India, South Africa, China and Malaysia to treat diabetes. The present study reports the in vitro antioxidant and antidiabetic activities of the major alkaloids isolated from Catharanthus roseus (L.) G. Don leaves extract. Four alkaloids—vindoline I, vindolidine II, vindolicine III and vindolinine IV—were isolated and identified from the dichloromethane extract (DE) of this plant’s leaves. DE and compounds I–III were not cytotoxic towards pancreatic β-TC6 cells at the highest dosage tested (25.0 µg/mL). All four alkaloids induced relatively high glucose uptake in pancreatic β-TC6 or myoblast C2C12 cells, with III showing the highest activity. In addition, compounds II–IV demonstrated good protein tyrosine phosphatase-1B (PTP-1B) inhibition activity, implying their therapeutic potential against type 2 diabetes. III showed the highest antioxidant potential in ORAC and DPPH assays and it also alleviated H2O2-induced oxidative damage in β-TC6 cells at 12.5 µg/mL and 25.0 µg/mL. View Full-TextKeywords:
Catharanthus roseus; alkaloids; β-TC6; C2C12; cytotoxicity; antioxidant; glucose uptake; PTP-1B
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Tiong, S.H.; Looi, C.Y.; Hazni, H.; Arya, A.; Paydar, M.; Wong, W.F.; Cheah, S.-C.; Mustafa, M.R.; Awang, K. Antidiabetic and Antioxidant Properties of Alkaloids from Catharanthus roseus (L.) G. Don. Molecules 2013, 18, 9770-9784.
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