Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells
1
Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China
2
Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China
3
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
4
School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
*
Author to whom correspondence should be addressed.
Molecules 2012, 17(3), 3155-3164; https://doi.org/10.3390/molecules17033155
Received: 6 February 2012
/
Revised: 28 February 2012
/
Accepted: 2 March 2012
/
Published: 12 March 2012
Astragalus polysaccharides (APS), one of main bioactive components in Astragalus membranaceus Bunge, has been reported to possess anti-inflammatory activities, but the molecular mechanisms behind this activity are largely unknown. This study aimed to investigate expression of inflammatory cytokines and the MAPK/NF-κB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS). The results showed that the concentrations of TNF-a and IL-1β released from LPS stimulated THP-1 cells increased significantly compared to control (p < 0.01). After treatment with APS, the TNF-a and IL-1β levels were significantly lower than those in the LPS group (p < 0.05). The mRNA expression of TNF-a and IL-1β were also inhibited. Mechanistic studies indicated that APS strongly suppressed NF-κB activation and down-regulated the phosphorylation of ERK and JNK, which are important signaling pathways involved in the production of TNF-a and IL-1β, demonstrating that APS could suppress the production of TNF-a and IL-1β by LPS stimulated macrophages by inhibiting NF-κB activation and ERK and JNK phosphorylation.
View Full-Text
Keywords:
Astragalus polysaccharides; anti-inflammatory; macrophage; MAPK; NF-κB
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License
MDPI and ACS Style
He, X.; Shu, J.; Xu, L.; Lu, C.; Lu, A. Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells. Molecules 2012, 17, 3155-3164. https://doi.org/10.3390/molecules17033155
AMA Style
He X, Shu J, Xu L, Lu C, Lu A. Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells. Molecules. 2012; 17(3):3155-3164. https://doi.org/10.3390/molecules17033155
Chicago/Turabian StyleHe, Xiaojuan, Jun Shu, Li Xu, Cheng Lu, and Aiping Lu. 2012. "Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells" Molecules 17, no. 3: 3155-3164. https://doi.org/10.3390/molecules17033155
Find Other Styles
