Spiculisporic Acids B–D, Three New γ-Butenolide Derivatives from a Sea Urchin-Derived Fungus Aspergillus sp. HDf2

Three new γ-butenolide derivatives 1–3, named spiculisporic acids B–D, were isolated from the culture of Aspergillus sp. HDf2, a marine-derived fungus that resides in the sea urchin, Anthocidaris crassispina. The structures of 1–3 were elucidated on the basis of spectroscopic methods, including MS and 2D NMR techniques. Their in vitro cytotoxic activities against two cell lines (SGC-7901, human gastric adenocarcinoma and SPC-A-1, human lung adenocarcinoma) and inhibitory activities against Staphylococcus aureus ATCC 51650 were investigated.


Introduction
Microorganisms of marine origin have proven to be a rich source of novel and/or biologically active natural products with promising pharmacological properties [1][2][3][4].In related studies, the Tsukamoto laboratory reported a new antimicrobial anthraquinone (monodictyquinone A) from a sea urchin-derived fungus Monodictys sp.[5].In our search for new natural products from marine-derived microorganisms, the fungus Aspergillus sp.HDf2 was isolated from the sea urchin Anthocidaris crassispina, collected from the seashore of Qionghai, Hainan, China.The genus Aspergillus (Trichocomaceae) is one of the most prolific fungi that produce a variety of secondary metabolites with novel structures and interesting bioactivities [6].Subsequent chemical study on the fermentation broth of the fungus Aspergillus sp.HDf2 led to the isolation of three new γ-butenolide derivatives, the structures of which were similar to that of spiculisporic acid [7,8], and these compounds were thus named as spiculisporic acids B-D (compounds 1-3, Figure 1).Their structures were elucidated by comprehensive spectroscopic analyses.Herein we report the isolation, structural determination, bioactivities of these new natural products.

Results and Discussion
Spiculisporic acid B (1) was isolated as a white solid, with the molecular formula C 17 H 26 O 6 (five degrees of unsaturation) as derived from ESI high-resolution mass spectrometry ([M−H] − at m/z 325.1664, calculated 325.1657) and 1 H-and 13 C-NMR spectral data (Tables 1 and 2).Together, these data indicate that compound 1 has one double bond and three carbonyls, which account for 4 out of the 5 degrees of unsaturation required by the molecular formula, so spiculisporic acid B must contain a ring.The structural information for 1 was determined from a series of 2D NMR analyses, including HSQC, 1 H-1 H COSY, and HMBC spectra (Figure 2).The 1 H-1 H COSY experiment revealed a correlation between H-2 (δ H 2.60) and H-3 (δ H 2.49), and a seperate spin system, H 2 C=CH-CH 2 -(CH 2 ) 6 -CH 2 -CH-.The methine proton H-14 was coupled with the methylene protons H-13 (δ H 2.04) and H-15, and the methylene proton H-13 was coupled with the methylene proton at H-12 (δ H 1.37).The correlations between the methylene proton H-6a (δ H 1.85) and H-5 [δ H 3.01 (br d, J = 9.2 Hz)] and H-7 (δ H 1.25-1.37)were observed in the 1 H-1 H COSY spectrum.HMBC correlations from H-5 to C-4 (δ C 88.2) and two carbonyl carbons (δ C 175.5 and 174.2), from H-3 to C-1 (δ C 178.5), C-4, C-5 (δ C 52.5), and one of the carbonyl carbons (δ C 174.2), and from H-2 to C-1 and C-4, were observed.These observations allowed the structure of 1 to be determined as shown in Figure 1.This formula differed by the addition of CH 2 to the molecular formula of spiculisporic acid [7,8], suggesting an additional methylene or methyl group had been added to the structure.The 1 H and 13 C-NMR data for 2 were almost identical to those of spiculisporic acid, except for the presence of a new oxygenated methyl group (δ C 53.6, δ H 3.80).The HMBC spectrum showed a strong correlation between the oxygenated methyl protons and carbonyl carbon at δ C 172.8, which was correlated with H-3 (δ H 2.48), thus indicating the position of the methoxyl group.Based on the HSQC, 1 H-1 H COSY, and HMBC analyses of 2 (Figure 2), and the good comparison of NMR data of C-4 (δ C 88.2) and C-5 (δ C 52.9) in 2 to those from (−)-spiculisporic acid, we proposed the structure of 2 to be (4S,5S)-4-(5-carboxylundecyl)-1-oxo-tetrahydrofuran-4-carboxyl acid methyl ester, named spiculisporic acid C. It was possible that the methoxyl group in 2 was a result of a reaction with methanol in the procedure of isolation.
Spiculisporic acid D (3), isolated as a pale white solid, gave a [M+Na] + ion peak at m/z 365.1940 in its positive-mode HR-ESI-MS, indicating its molecular formula to be C 18 H 30 O 6 , which was the same as that of 2. Through detailed analyses of the 1 H-and 13 C-NMR spectra of 3, the major difference between 3 and spiculisporic acid was the presence of a new methylene group in the aliphatic chain.Unambiguous assignments of 1 H-and 13 C-NMR data were obtained by interpretation of HSQC, 1 H-1 H COSY, and HMBC data (Figure 2), confirming the structure for 3 as shown.The absolute configuration of 3, determined by comparison of NMR data of C-4 (δ C 88.7) and C-5 (δ C 52.7) in 3 with those of (−)-spiculisporic acid, was elucidated as (4S,5S)-4-(5-carboxyl-dodecyl)-1-oxotetrahydrofuran-4-carboxyl acid, named spiculisporic acid D.
Compounds 1-3 were subjected to cytotoxic activity tests against two cell lines, SGC-7901 and SPC-A-1 by MTT methods [9].However, none of these compounds were active with IC 50 > 50 μg/mL.Compounds 1-3 showed antibacterial activities against Staphylococcus aureus ATCC 51650 with inhibition zone of 9.6, 11.6, and 11.5 mm at 20 mg/mL, while the diameter of inhibition zone of the positive control was 23.6 mm.Spiculisporic acid, a fermentation adduct from the culture broth of Penicillium spiculisporum has found potential use as new controlled release carriers of active chemicals [7], and commercial application as a biosurfactant for metal decontamination processes to remove hard, large metal cations from water [10].These interesting properties of spiculisporic acids B-D are currently under investigation.

General Experimental Procedures
Optical rotations were taken on a Rudolph Autopol III.UV spectra were measured on a Hitachi U-3000 spectrophotometer, and IR spectra (KBr) were obtained on a Nicolet 380 FT-IR spectrometer.NMR spectra were recorded on a Bruker AVIII-500 spectrometer at 500 MHz for 1 H-NMR and at 125 MHz for 13 C-NMR.Chemical shifts are given in δ (ppm) and referenced to the solvent signal (methanol-d 4 , δ H 3.31, δ C 49.1) as the internal standard, and coupling constants (J) are reported in Hz.HR-ESI-MS spectra were recorded on a Agilent 6210 TOF LC/MS mass spectrometer.Silica gel (200-300 mesh) for column chromatography (CC) and silica GF 254 (10-20 mm) for TLC were obtained from Qingdao Marine Chemical Factory (Qingdao, China).YMC ODS gel (50 μm) was purchased from Shanghai HANKING Instrument & Equipment Co., Ltd.(Shanghai, China) Sephadex LH-20 for chromatography was purchased from Merck (Darmstadt, Germany).Semipreparative HPLC was performed on a Hitachi L-7110 pump, and UV detector L-7400 equipped with a Waters ODS column (5 μm, 250 × 4.6 mm).

Fungal Material and Cultivation
The fungus Aspergillus sp.HDf2 was isolated and identified by one of the authors (R.W.) from the gut of a healthy sea urchin Anthocidaris crassispina collected from the seashore of Qionghai, Hainan, China, in October 2009.A voucher specimen with the code HNF-HD02 is deposited in the Hainan Provincial Fisheries Research Institute.The fungus was cultivated on MEA solid medium composed of 20 g/L malt extract, 20 g/L sucrose, 1 g/L peptone, 20 g/L agar and deionized water for 5 days at 28 °C.Agar plugs were used to inoculate in 1000-mL Erlenmeyer flasks, each containing 300 mL of ME liquid media.Fermentation was carried out on a rotary shaker (140 rpm) at 26 °C for 12 days in 40 × 1,000 mL Erlenmeyer flasks.

In Vitro Cytotoxicity Test
The cytotoxic activities for compounds 1-3 were tested in vitro against two cell lines, SGC-7901 (human gastric adenocarcinoma) and SPC-A-1 (human lung adenocarcinoma), which were purchased from the Jiangsu Provincial Center for Disease Prevention and Control.The purity of the tested compounds and doxorubicin•HCl was determined to be over 95% by using the HPLC-DAD method.The cytotoxic in vitro effects on these tested cell were assessed by the IC 50 values, and determined by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] colometric method [8].Each set of test was conducted three times to confirm reproducibility of the results.The compounds were dissolved in DMSO (dimethyl sulfoxide).Doxorubicin•HCl was used as a positive control, and the medium without test compound as a negative control in the bioassay.

Antibacterial Test
Compounds 1-3 were tested for in vitro antimicrobial activity against Staphylococcus aureus ATCC51650 by the filter paper disc agar diffusion method.The NA medium was mixed with 2 mL of suspension containing 1 × 10 5 ~ 1 × 10 7 cfu/mL of Staphylococcus aureus, and then poured into petri-plates.2 μL 20 mg/mL of the isolated compounds dissolved in DMSO were impregnated on sterile filter paper discs (6 mm diameter) and then were applied on the surface of the solidified agar plates.Every sample was tested in triplicate.Streptomycin sulfate (2 μL, 20 mg/mL) was used as positive control.The test plates were incubated at 37 °C for 24 h.Then the diameters of the inhibition zones including the 6 mm disc diameter were measured.

Conclusions
In our screening for new secondary metabolites from marine-derived fungi associated with marine animals from the coast of Hainan Island, three new secondary metabolites 1-3, named spiculisporic
a−e Overlapping signals.f−h Interchangeable signals.