A Curcumin Derivative, 2,6-Bis(2,5-dimethoxybenzylidene)-cyclohexanone (BDMC33) Attenuates Prostaglandin E2 Synthesis via Selective Suppression of Cyclooxygenase-2 in IFN-g/LPS-Stimulated Macrophages
Abstract
:Abbreviations
BDMC33 | 2,6-bis(2,5-dimethoxybenzylidene)cyclohexanone |
COX | cyclooxygenase |
DMSO | dimethyl sulfoxide |
DXM | dexamethasone |
FBS | foetal bovine serum |
HRP | horseradish peroxidase |
IFN-γ | interferon-gamma |
LPS | lipopolysaccharide |
NO | nitric oxide |
PGE2 | prostaglandin E2 |
NSAID | non-steroidal anti-inflammatory drug |
RAW 264.7 | murine macrophage cells |
1. Introduction
2. Results and Discussion
2.1. Effects of BDMC33 on PGE2 Secretion and Cell Viability
2.2. Effects of BDMC33 on COX-2 Enzymatic Activity
2.3. Effects of BDMC33 on COX Expression
3. Experimental
3.1. Materials
3.2. Synthesis of 2,6-Bis(2,5-dimethoxybenzylidene)cyclohexanone (BDMC33)
3.3. Cell Culture
3.4. Cell Stimulation and Treatment
3.5. Cell Viability
3.6. Determination of PGE2
3.7. Indirect Determination of Endogenous COX-2 Activity
3.8. Western Blotting Analysis of COX Expression
3.9. Statistical Analysis
4. Conclusions
Acknowledgment
References
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Lee, K.-H.; Abas, F.; Alitheen, N.B.M.; Shaari, K.; Lajis, N.H.; Ahmad, S. A Curcumin Derivative, 2,6-Bis(2,5-dimethoxybenzylidene)-cyclohexanone (BDMC33) Attenuates Prostaglandin E2 Synthesis via Selective Suppression of Cyclooxygenase-2 in IFN-g/LPS-Stimulated Macrophages. Molecules 2011, 16, 9728-9738. https://doi.org/10.3390/molecules16119728
Lee K-H, Abas F, Alitheen NBM, Shaari K, Lajis NH, Ahmad S. A Curcumin Derivative, 2,6-Bis(2,5-dimethoxybenzylidene)-cyclohexanone (BDMC33) Attenuates Prostaglandin E2 Synthesis via Selective Suppression of Cyclooxygenase-2 in IFN-g/LPS-Stimulated Macrophages. Molecules. 2011; 16(11):9728-9738. https://doi.org/10.3390/molecules16119728
Chicago/Turabian StyleLee, Ka-Heng, Faridah Abas, Noorjahan Banu Mohamed Alitheen, Khozirah Shaari, Nordin Haji Lajis, and Syahida Ahmad. 2011. "A Curcumin Derivative, 2,6-Bis(2,5-dimethoxybenzylidene)-cyclohexanone (BDMC33) Attenuates Prostaglandin E2 Synthesis via Selective Suppression of Cyclooxygenase-2 in IFN-g/LPS-Stimulated Macrophages" Molecules 16, no. 11: 9728-9738. https://doi.org/10.3390/molecules16119728