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Roles of JNK/Nrf2 Pathway on Hemin-Induced Heme Oxygenase-1 Activation in MCF-7 Human Breast Cancer Cells

1
Department of Biochemistry and Institute of Cardiovascular Research, Chonbuk National University Medical School, Jeonju 54896, Korea
2
Department of Anesthesiology and Pain Medicine, Bucheon St. Mary’s Hospital, Catholic University of Korea, Bucheon 14647, Korea
3
Department of Emergency Medical Rescue, Nambu University, Gwangju 62271, Korea
4
Department of Emergency Medicine, Graduate School of Chonnam National University, Gwangju 61469, Korea
*
Authors to whom correspondence should be addressed.
Medicina 2020, 56(6), 268; https://doi.org/10.3390/medicina56060268
Received: 14 April 2020 / Revised: 23 May 2020 / Accepted: 27 May 2020 / Published: 29 May 2020
Heme oxygenase-1 (HO-1) is highly induced in various human disease states, including cancer, indicating that HO-1 is an emerging target of cancer therapy. In this study, we investigated that the mechanisms of hemin-induced HO-1 expression and its signaling pathways in human breast cancer cell. We used MCF-7 cells, a human breast cancer cell line. Hemin increased HO-1 expression in MCF-7 cells in a dose- and time-dependent manner. Hemin enhanced HO-1 expression through the activation of c-Jun N-terminal kinases (JNK) signaling pathway. Hemin also induced activation of Nrf2, a major transcription factor of HO-1 expression. These responses in MCF-7 cells were completely blocked by pretreatment with brazilin, a HO-1 regulator. These results indicated that brazilin inhibits hemin-induced HO-1 expressions through inactivation of JNK/Nrf2 in MCF-7 cells. Thus, our findings suggest that HO-1 is an important anticancer-target of brazilin in human breast cancer. View Full-Text
Keywords: heme oxygenase-1; JNK; Nrf2; breast cancer; MCF-7; hemin; brazilin heme oxygenase-1; JNK; Nrf2; breast cancer; MCF-7; hemin; brazilin
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Jang, H.-Y.; Hong, O.-Y.; Chung, E.-Y.; Park, K.-H.; Kim, J.-S. Roles of JNK/Nrf2 Pathway on Hemin-Induced Heme Oxygenase-1 Activation in MCF-7 Human Breast Cancer Cells. Medicina 2020, 56, 268.

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