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Open AccessArticle

Bone Marrow Mesenchymal Stromal Cells (BMMSCs) Augment Osteointegration of Dental Implants in Type 1 Diabetic Rabbits: An X-Ray Micro-Computed Tomographic Evaluation

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Department of Periodontics and Community Dentistry, College of Dentistry, King Khalid University, Abha 62529, Saudi Arabia
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Centre for Stem Cell Research and Dept. of Microbiology and Clinical Parasitology, College of Medicine, King Khalid University, Abha 62529, Saudi Arabia
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Department of Prosthetic Dentistry, College of Dentistry, King Khalid University, Abha 62529, Saudi Arabia
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Department of Oral Basic and Clinical Sciences, Faculty of Dentistry, King Abdul Aziz University, Jeddah 21589, Saudi Arabia
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Department of Prosthodontics, Raja Rajeshwari Dental College and Hospital, Bengaluru 560074, India
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Rajah Muttiah Dental College and Hospital, Annamalai University, Chidambaram 608002, India
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Author to whom correspondence should be addressed.
Medicina 2020, 56(4), 148; https://doi.org/10.3390/medicina56040148
Received: 18 February 2020 / Revised: 19 March 2020 / Accepted: 19 March 2020 / Published: 25 March 2020
(This article belongs to the Special Issue Dental Implants and Biomaterials Innovations)
Background and objectives: The study aimed to investigate the effect of bone marrow mesenchymal stromal cells (BMMSCs) on implant-bone osseointegration in type I diabetic New Zealand rabbits. Materials and methods: BMMSCs harvested from healthy rabbits were processed and validated for purity and osteocyte differentiability. Mandibular incisors of diabetic and control rabbits were carefully extracted, and the sockets were plugged with collagen sponges. Platelet-rich plasma (PRP) containing osteoinductive BMMSCs, and plain PRP were injected into the collagen sponge of the right and left sockets respectively. Dental implants of 2.6 mm diameter and 10 mm length were inserted into the collagen sponge of both sockets. All the animals were sacrificed six weeks post surgery to evaluate an early stage of osseointegration; the mandibles scanned by X-ray microcomputed tomography (μCT) and subjected to 3D analysis. The μCT parameters of the right implant were paired against that of the left side of each animal and analyzed by paired T-test. Results: The preclinical evaluation of the viability and osteocyte differentiation of the BMMSCs were consistent between both the donor samples. The osseointegration of dental implants with stem cell therapy (BMMSCs + PRP + collagen) in normal and diabetic rabbits was significantly higher than that of implants with adjunctive PRP + collagen only (p < 0.05). Conclusion: Stem Cell therapy with osteoinductive BMMSCs and PRP can offer a novel approach to enhance the osseointegration of dental implants in uncontrolled diabetic patients. View Full-Text
Keywords: osseointegration; stem cells; diabetes mellitus; x-ray micro-computed tomography (micro-ct); dental implants osseointegration; stem cells; diabetes mellitus; x-ray micro-computed tomography (micro-ct); dental implants
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Abdullah Alqahtani, N.; C. Chandramoorthy, H.; Shaik, S.; Syed, J.; Chowdhary, R.; Antony, L. Bone Marrow Mesenchymal Stromal Cells (BMMSCs) Augment Osteointegration of Dental Implants in Type 1 Diabetic Rabbits: An X-Ray Micro-Computed Tomographic Evaluation. Medicina 2020, 56, 148.

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