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Open AccessArticle

Combined Effect of HPV and Several Gene SNPs in Laryngeal Cancer

1
Biobank, National Cancer Institute, P. Baublio 3b, LT-08406 Vilnius, Lithuania
2
Laboratory of Clinical Oncology, National Cancer Institute, P. Baublio 3b, LT-08406 Vilnius, Lithuania
3
Department of Head and Neck Surgery and Oncology, National Cancer Institute, Santariškių 1, LT-08660 Vilnius, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2020, 56(2), 81; https://doi.org/10.3390/medicina56020081
Received: 26 December 2019 / Revised: 11 February 2020 / Accepted: 12 February 2020 / Published: 17 February 2020
Background and objectives: Laryngeal squamous cell carcinoma (LSCC) is one of the most common head and neck tumors. The molecular mechanism of LSCC remains unclear. The aim of this study was to evaluate the prevalence of Human papillomavirus (HPV) and single nucleotide polymorphisms (SNPs) of TP53, MDM2, MDM4, MTHFR, CASP8, and CCR5 genes in LSCC, and to assess their correlations with patient survival. Materials and Methods: 49 LSCC patients were enrolled in this study. PCR and qRT-PCR were used to detect, identify, and quantify HPV. SNPs were genotyped using PCR and PCR-RFLP. Results: By analyzing the interactions of the SNPs of the genes with clinical parameters, the majority of patients with lymph node status (N1,2) were identified as carriers of MDM2 T/G, CASP8 ins/del, CCR5 wt/wt SNP. Cluster analysis showed that patients with MDM2 T/T SNP survive longer than patients identified as CASP8 ins/ins, MTHFR C/C, and MDM4 A/A variant carriers; meanwhile, LSCC patients with MDM2 T/T polymorphic variant had the best survival. Multivariate analysis showed that HPV-positive patients without metastasis in regional lymph nodes (N0) and harboring CASP8 ins/del variant had the best survival. Meanwhile, HPV-negative patients with identified metastasis in lymph nodes (N1 and N2) and CASP8 ins/del variant had poor survival. Conclusions: This finding suggests patients survival prognosis and tumor behavior are different according HPV status, SNP variants, and clinical characteristics of the LSCC. View Full-Text
Keywords: laryngeal squamous cell carcinoma; single nucleotide polymorphisms; patient survival; human papillomavirus; multivariate analysis laryngeal squamous cell carcinoma; single nucleotide polymorphisms; patient survival; human papillomavirus; multivariate analysis
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Stumbrytė-Kaminskienė, A.; Gudlevičienė, Ž.; Dabkevičienė, D.; Mackevičienė, I. Combined Effect of HPV and Several Gene SNPs in Laryngeal Cancer. Medicina 2020, 56, 81.

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