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Open AccessArticle

A Retrospective Study about the Impact of Switching from Nested PCR to Multiplex Real-Time PCR on the Distribution of the Human Papillomavirus (HPV) Genotypes

1
Section of Microbiology, Interdisciplinary Department of Medicine (DIM), School of Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy
2
UOC Microbiology and Virology, Azienda Ospedaliera-Universitaria Policlinico of Bari, 70124 Bari, Italy
3
Pediatric Clinical Research Center “Romeo and Erica Invernizzi”, University of Milan, 20157 Milan, Italy
4
Department of Biology and Biotechnology, University of Pavia, 27100 Pavia, Italy
5
Ionian Department, University of Bari “Aldo Moro”, 70100 Bari, Italy
*
Author to whom correspondence should be addressed.
Medicina 2019, 55(8), 418; https://doi.org/10.3390/medicina55080418
Received: 19 June 2019 / Revised: 19 July 2019 / Accepted: 26 July 2019 / Published: 30 July 2019
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Abstract

Background and objectives: Human papillomavirus (HPV) is the most prevalent etiological agent of viral sexually-transmitted infection. This study retrospectively evaluated the impact of a switch to a real-time PCR assay in the HPV prevalence and genotypes distribution by a quasi-experimental before-and-after approach. Materials and Methods: In total, 1742 samples collected from 1433 patients were analyzed at the UOC Microbiology and Virology of Policlinico of Bari, Italy. HPV DNA detection was performed using initially nested PCR and subsequently multiplex real-time PCR assay. Results: Statistically significant difference in HPV overall prevalence after the introduction of the real-time assay was not detected (48.97% vs. 50.62%). According to different extraction-DNA amplification methods, differences were observed in the prevalence rates of HPV-45, 68, 40, 42, and 43. The lowest prevalence for HPV-45 was observed in the Magna Pure-Real Time PCR group, while HPV-68, 40, 42, and 43 were less observed in the Qiagen-Real Time PCR group. After, a multivariate logistic regression, an increase in the prevalence of HPV-42 (aOR: 4.08, 95% CI: 1.71–9.73) was associated with the multiplex real-time PCR assay. Conclusions: Although this study is a not a direct comparison between two diagnostic methods because it has a sequential structure, it serves to verify the impact of a new molecular assay on HPV distribution. Moreover, the stability of HPV prevalence over time suggests that the population composition and the behavioral variables did not likely change during the observation period. Our study proposes that the introduction of a molecular test for HPV detection may be related to changes of HPV genotypes distribution. View Full-Text
Keywords: HPV; sexually transmitted diseases (STDs); laboratory methods; PCR; genotypes; surveillance; epidemiology HPV; sexually transmitted diseases (STDs); laboratory methods; PCR; genotypes; surveillance; epidemiology
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Del Prete, R.; Ronga, L.; Addati, G.; Magrone, R.; Abbasciano, A.; Di Carlo, D.; Santacroce, L. A Retrospective Study about the Impact of Switching from Nested PCR to Multiplex Real-Time PCR on the Distribution of the Human Papillomavirus (HPV) Genotypes. Medicina 2019, 55, 418.

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