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Phytochemical Analysis, Ephedra Procera C. A. Mey. Mediated Green Synthesis of Silver Nanoparticles, Their Cytotoxic and Antimicrobial Potentials

1
Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan
2
Department of Eastern Medicine and Surgery, Qarshi University, Lahore 54000, Pakistan
3
State Key Laboratory of Chemo/Biosensing and Chemometrics, Collage of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
4
Departant of Pharmacy, University of Malakand, Khyber Pakhtunkhwa 18800, Pakistan
5
Lahore campus, Qarshi University, Lahore 54000, Pakistan
6
Pakistan Academy of Sciences, Islamabad 44000, Pakistan
*
Author to whom correspondence should be addressed.
Medicina 2019, 55(7), 369; https://doi.org/10.3390/medicina55070369
Received: 29 April 2019 / Revised: 4 July 2019 / Accepted: 10 July 2019 / Published: 12 July 2019
(This article belongs to the Special Issue Nano-Biosciences in the Field of Health-Care)
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Abstract

Background and Objectives: The current study focuses on an eco-friendly and cost-effective method of Ephedra procera C. A. Mey. mediated green synthesis of silver nanoparticles as potential cytotoxic, antimicrobial and anti-oxidant agents. Materials and Methods: Plant aqueous extracts were screened for Total Phenolic (TPC), Total Flavonoid contents (TFC), Total Antioxidant Capacity (TAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging potentials. Total reducing power estimated by potassium ferricyanide colorimetric assay. The biosynthesized E. procera nanoparticles (EpNPs) were characterized by UV-spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction and Scanning electron microscopy. EpNPs were evaluated for their antimicrobial, bio-compatibility and cytotoxic potentials. Results: Initial phytocheimcal analysis of plant aqueous extract revealed TFC of 20.7 ± 0.21 µg/mg extract and TPC of 117.01 ± 0.78 µg/mg extract. TAC, DPPH free radical scavenging and reducing power were 73.8 ± 0.32 µg/mg extract, 71.8 ± 0.73% and 105.4 ± 0.65 µg/mg extract respectively. The synthesized EpNPs were observed to possess high cytotoxicity against HepG2 cancer cell lines with IC50 (61.3 µg/mL) as compared aqueous extract with IC50 of (247 µg/mL). EpNPs were found to be biocompatible and have less effect on human erythrocytes. EpNPs exhibited significant antioxidant potentials and exhibited considerable activity against Escherichia coli and Bacillus subtilis with Minimum Inhibitory Concentration (MICs) of 11.12 μg/mL and 11.33 μg/mL respectively. Fungal species Aspergillus niger and Aspergillus flavus were found susceptible to EpNPs. Conclusions: Results of the current study revealed that EpNPs exhibited considerable antibacterial, antifungal and cytotoxic potentials. Aqueous extract possesses significant anti-radical properties and thus can be useful in free radicals induced degenerative disorders. View Full-Text
Keywords: Ephedra procera; green chemistry; microbial resistance; HepG2 cells; hemolysis; nanotechnology Ephedra procera; green chemistry; microbial resistance; HepG2 cells; hemolysis; nanotechnology
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Nasar, M.Q.; Khalil, A.T.; Ali, M.; Shah, M.; Ayaz, M.; Shinwari, Z.K. Phytochemical Analysis, Ephedra Procera C. A. Mey. Mediated Green Synthesis of Silver Nanoparticles, Their Cytotoxic and Antimicrobial Potentials. Medicina 2019, 55, 369.

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