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Arabinoxylan-Based Particles: In Vitro Antioxidant Capacity and Cytotoxicity on a Human Colon Cell Line

1
Biopolymers, Research Center for Food and Development (CIAD), Hermosillo, Sonora 83304, Mexico
2
Biotechnology, Research Center for Food and Development (CIAD), Hermosillo, Sonora 83304, Mexico
3
Nutrition, Research Center for Food and Development (CIAD), Hermosillo, Sonora 83304, Mexico
4
Department of Chemical Biological and Agropecuary Sciences, University of Sonora, Caborca, Sonora 83621, Mexico
5
Department of Polymers and Materials, University of Sonora, Hermosillo, Sonora 83000, Mexico
6
Department of Chemistry-Biology, University of Sonora, Hermosillo, Sonora 83000, Mexico
*
Author to whom correspondence should be addressed.
Medicina 2019, 55(7), 349; https://doi.org/10.3390/medicina55070349
Received: 5 June 2019 / Revised: 29 June 2019 / Accepted: 4 July 2019 / Published: 7 July 2019
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Abstract

Background and objectives: Arabinoxylans (AX) can gel and exhibit antioxidant capacity. Previous studies have demonstrated the potential application of AX microspheres as colon-targeted drug carriers. However, the cytotoxicity of AX gels has not been investigated so far. Therefore, the aim of the present study was to prepare AX-based particles (AXM) by coaxial electrospraying method and to investigate their antioxidant potential and cytotoxicity on human colon cells. Materials and Methods: The gelation of AX was studied by monitoring the storage (G′) and loss (G′′) moduli. The morphology of AXM was evaluated using optical and scanning electron microscopy (SEM). The in vitro antioxidant activity of AX before and after gelation was measured using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. In addition, the effect of AX and AXM on the proliferation of human colon cells (CCD 841 CoN) was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The final G′ and G′′ values for AX gels were 293 and 0.31 Pa, respectively. AXM presented spherical shape and rough surface with a three-dimensional and porous network. The swelling ratio and mesh size of AXM were 35 g water/g AX and 27 nm, respectively. Gelation decreased the antioxidant activity of AX by 61–64 %. AX and AXM did not affect proliferation or show any toxic effect on the normal human colon cell line CCD 841 CoN. Conclusion: The results indicate that AXM could be promising biocompatible materials with antioxidant activity. View Full-Text
Keywords: arabinoxylan gel; microspheres; ferulic acid; antioxidant activity; cytotoxicity; microstructure; coaxial electrospraying arabinoxylan gel; microspheres; ferulic acid; antioxidant activity; cytotoxicity; microstructure; coaxial electrospraying
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Mendez-Encinas, M.A.; Carvajal-Millan, E.; Rascón-Chu, A.; Astiazarán-García, H.; Valencia-Rivera, D.E.; Brown-Bojorquez, F.; Alday, E.; Velazquez, C. Arabinoxylan-Based Particles: In Vitro Antioxidant Capacity and Cytotoxicity on a Human Colon Cell Line. Medicina 2019, 55, 349.

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