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Hepatoprotective Effect of Melatonin in Toxic Liver Injury in Rats

1
Department of Pharmacology and Clinical Pharmacology, I. Horbachevsky Ternopil State Medical University, Maidan Voli sq. 1, 46001 Ternopil, Ukraine
2
Department of orthopedagogy and physical therapy, Ternopil V. Hnatiuk National Pedagogical University, Maxym Kryvonis str. 2, 46027 Ternopil, Ukraine
3
Department of Medical Biochemistry, I. Horbachevsky Ternopil State Medical University, Maidan Voli sq 1., 46001 Ternopil, Ukraine
4
Central Scientific Research Laboratory, I. Horbachevsky Ternopil State Medical University, Maidan Voli sq. 1, 46001 Ternopil, Ukraine
*
Author to whom correspondence should be addressed.
Medicina 2019, 55(6), 304; https://doi.org/10.3390/medicina55060304
Received: 19 April 2019 / Revised: 17 June 2019 / Accepted: 18 June 2019 / Published: 24 June 2019
(This article belongs to the Section Hepatology)
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Abstract

Background and objectives: toxic liver injury results in nitrooxidative stress. Melatonin is a potent free radical scavenger, an inducible nitric oxide synthase (iNOS) inhibitor and an activator of antioxidant enzymes. The aim of this study was to investigate the hepatoprotective effect of exogenous melatonin on animals with acute toxic hepatitis. Material and methods: 36 healthy Sprague-Dawley male rats were split into three equal groups and given carbon tetrachloride (CCl4), 2 g/kg (CCl4 group) or the same dose of CCl4 and melatonin, 10 mg/kg (CCl4/melatonin group) or saline (control group). The effect of melatonin on prooxidant and antioxidant system indexes, NO and NOS levels in serum and liver, data of mitochondrial chain functions and cytolysis in liver were evaluated in all three groups. Results: melatonin significantly decreased activities of AST, ALT, ceruloplasmine and thiobarbituric acid reactive substance (TBARS) in serum. Catalase activity was lowered in serum but not in the liver. Hepatic TBARS, lipid hydroperoxides and glutathione concentrations were decreased, while superoxide dismutase, mitochondrial cytochrome oxidase and succinate dehydrogenase activities increased. Melatonin inhibited synthesis of stable NO metabolites in serum: NO2-by 37.9%; NO3-by 29.2%. There was no significant difference in content NO2-in the liver, but concentration of NO3-increased by 32.6%. Melatonin significantly reduced iNOS concentrations both in serum (59.7%) and liver (57.8%) but did not affect endothelial isoform enzyme activities neither in serum, nor in liver. The histopathological liver lesions observed in the CCl4/melatonin group were less severe than those seen in the CCl4 group. Conclusions: we demonstrated an ameliorating effect of melatonin on prooxidants and antioxidants, NO-NOS systems balance, mitochondrial function and histopathological lesions in the liver in rats with CCl4-induced hepatitis. View Full-Text
Keywords: rat; melatonin; liver; lipoperoxidation; antioxidant; nitric oxide rat; melatonin; liver; lipoperoxidation; antioxidant; nitric oxide
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Oleshchuk, O.; Ivankiv, Y.; Falfushynska, H.; Mudra, A.; Lisnychuk, N. Hepatoprotective Effect of Melatonin in Toxic Liver Injury in Rats. Medicina 2019, 55, 304.

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